Stem Cell Signaling Compound Library
Stem Cell Signaling Compound Library目录
Effects of CP-690550 on NKTCL cell lines. NK-S1, KHYG-1 cells were treated with CP-690550 for 48 hours, and the effect on STAT5 phosphorylation was evaluated by Western blotting.
Cancer Discov 2012 2(7), 591-7. Tofacitinib (CP-690550,Tasocitinib) purchased from Selleck
Hh signaling controls metabolic reprogramming during liver injury in vivo. Immunohistochemistry identifies cells expressing the M2 isozyme of PKM2, a specific marker of glycolytic activity, ( C) inhibited in aged MDR2 -/- mice by GDC-0449.
Gastroenterology 2012 143, 1319-29. Vismodegib (GDC-0449) purchased from Selleck
Tamoxifen was administered to adult Psmc1fl/wt and Psmc1fl/fl Pdgf-Cre-ER mice, followed by treatment with fasudil control (4 and 48 hours after tamoxifen). Representative images of Dylight 488-positive megakaryocytes present in crude bone marrow isolated from Psmc1fl/wt and Psmc1fl/flPdgf-Cre-ER mice immediately after euthanasia. Bone marrow for these studies was isolated from a subset of the mice in A (n=2 per treatment group). Scale bars: 100 um.
J Clin Invest 2014 124(9), 3757-66. Fasudil (HA-1077) HCl purchased from Selleck
The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.
J Clin Invest 2014 124(4), 1646-59. Fasudil (HA-1077) HCl purchased from Selleck
RU-SKI 43 blocks Shh signaling. (a) RU-SKI 43 blocks Gli activation. NIH 3T3 cells were cotransfected with vectors encoding 8× Gli-binding site (GliBS)-Firefly luciferase (unless indicated otherwise), Renilla luciferase reporter (pRL-TK) and Shh. Confluent cells were treated with DMSO, 10 μM LDE225, 10 μM RU-SKI 43 or 10 μM C-2. The firefly luciferase (FL)/Renilla luciferase (RL) ratio in cell lysates was calculated and normalized to that measured in DMSO-treated samples; error bars represent mean ± s.d. (n = 2–3).
Nat Chem Biol 2013 9, 247-9. LDE225 (NVP-LDE225,Erismodegib) purchased from Selleck
Fluorescence microscopy of pSuper or Cdo shRNA expressing P19 cells at ITS1 immunostained withβ-tubulin III antibodies. Size bar=100 um. P19/control or P19/Cdo shRNA cells were treated with DMSO or XAV939 in the differentiation medium for 72 h followed by immunostaining.
Nat Commun 2014 5, 5455. XAV-939 purchased from Selleck
STAT3 phosphorylation as determined by phospho flow, mixed lymphocyte reactions containing BALB/c spleen-derived CD4+ T cells co-cultured with or without C57BL/6 BM-derived DC preactivated with 20 ng/mL LPS.
Blood 2014 123(24), 3832-42. Ruxolitinib (INCB018424) purchased from Selleck
Colony-forming assay results showing that the Jak2 inhibitor TG101348 reduces CFU-GM colonies generated from mutant fetal liver R2 cells. Results from 4 independent control or mutant fetal livers treated with TG101348 or dimethylsulfoxide (DMSO) are shown (mean ?SD). ***P < .001.
Blood 2014 123(20), 3175-84. Fedratinib (SAR302503, TG101348) purchased from Selleck
• 干细胞抑制剂库含有与调节干细胞功能的信号通路相关的小分子，如Wnt, Notch, SMAD, 和Hedgehog 信号
|配制:||A collection of 89 small molecule inhibitors for stem cell research supplied as pre-dissolved DMSO solutions|
|容器:||96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode|