96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode

PI3K/Akt Inhibitor Library

A unique collection of 118 PI3K signaling pathway inhibitors

规格 价格  
预溶于DMSO
100uL/well (10mM solution) RMB 29422.19
2x100uL/well (10mM solution) RMB 44317.17
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产品详情

产品描述及优势

    • A unique collection of 118 small molecule inhibitors used for PI3K/Akt/mTOR pathway research.
    • A valuable tool for researching the survival, proliferation, and apoptosis of cells.
    • Targets PI3K, mTOR, Akt, GSK, ATM/ATR, etc.
    • Structurally diverse, medicinally active, and cell permeable.
    • Rich documentation with structure, IC50, and customer reviews.
    NMR and HPLC validated to ensure high purity.

产品详细信息

配制: A unique collection of 118 PI3K signaling pathway inhibitors supplied as pre-dissolved DMSO solutions
96-孔板: 96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode
稳定性:
1年 -20°C 溶于DMSO
2年 -80°C 溶于DMSO
发货: 蓝冰物流
包装: 惰性气体

化合物库组成成份

Chemical Library Composition

客户使用Selleck产品的实验数据 (10)

AS-605240 Review
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数据来源 Nat Genet,2014, 46(4), 364-70. Rapamycin (Sirolimus) purchased from Selleck
方法 Western blot
细胞系 Persister cells
浓度 10 nM
处理时间 3 days
结果 While added with a specific mTOR inhibitor, rapamycin(Rapa), it inhibitied endogenous mTOR activity, showed that markedly reduced MYC protein levels in persister cells but not in naive T-ALL cells.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Cell 2013, 24, 766-76. MK-2206 2HCl purchased from Selleck
方法 analysis of luciferase activity
细胞系 immunodeficient NOG mice
浓度 10 mg kg-1
处理时间 3 days
结果 In this experiment, animalstreated with dexamethasone or MK2206 showed progressivetumor growth similar to that observed in vehicle-treated controls,while mice treated with MK2206 plus dexamethasone had significant antitumor responses.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Cell 2012, 21(2), 155-67. Everolimus (RAD001) purchased from Selleck
方法 Western blotting
细胞系 MP tumor cells
浓度 1, 5 uM
处理时间 3 h
结果 To confirm that these compounds were acting on the PI3K/mTOR pathway, it performed western blotting to analyze phosphorylation of critical proteins in the pathway. As shown in figure, MP tumor cells showed substantial amounts of phospho-AKT and phospho-S6 in the absence of inhibitors (DMSO lanes). RAD-001 inhibited S6 phosphorylation but did not affect phospho-AKT. In contrast with RAD-001, treatment with BEZ-235 or BKM-120 inhibited phosphorylation of both AKT and S6.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Cell 2011, 19(6), 792-804. Rapamycin (Sirolimus) purchased from Selleck
方法 Cell viability Analysis
细胞系 Ar+murine (CaP8) and human (LNCaP) prostate cancer cells, Pb-Cre+;PtenL/L mice, Pb-Cre+;PtenL/L;ArL/Y mice
浓度 1 nM, 4 mg/kg
处理时间 0-4 weeks
结果 These data suggest that CaPs with AR loss have greater reliance upon the PI3K/AKT/mTOR-signaling pathways and that combined AR/androgen blockage in conjunction with PI3K/AKT/mTOR inhibition (by Rapamycin) is more effective for CaPs initiated by PTEN loss or PI3K/AKT activation.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Discov 2012, 2, 934-47. MK-2206 2HCl purchased from Selleck
方法 Western blot
细胞系 PC9GR4/WZR10 cells
浓度 1 uM
处理时间 72 h
结果 The addition of a dual PI3K and MTOR inhibitor, PI103, or the AKT inhibitor MK-2206, did reduce the expression of pAKT neither in GR4 cells nor WZR10 cells.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Discov 2012, 2, 1048-1063. KU-55933 (ATM Kinase Inhibitor) purchased from Selleck
方法 Western Blot
细胞系 HCC1937 cells
浓度 10 uM
处理时间 24 h
结果 As expected, KU-55933 led to a decrease in autophosphorylation of ATM ( Fig. 5A , third and fourth lane of each panel), and prevented the increase in H2AX phosphorylation seen in response to ionizing radiation. However, KU-55933 did not prevent the NVP-BKM120–induced induction of γ-H2AX, which was robust both at baseline and in response to ionizing radiation ( Fig. 5A , last lane of each panel), suggesting that an alternative kinase, such as DNA-PK, phosphorylates H2AX in response to PI3K inhibition.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Autophagy 2012, 8(6), 903-14. Torkinib (PP242) purchased from Selleck
方法 Immunofluorescence
细胞系 ARPE-19 cells
浓度 1 uM
处理时间 2 h
结果 It hypothesized that MTORC1 might regulate the distribution and activation of TFEB. To test this possibility we infected ARPE-19 cells with adenovirus expressing TFEB-Flag and treated the cells with PP242. As predicted, PP242 rapidly induced nuclear translocation of TFEB.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Autophagy 2012, 8(6), 903-14. Torkinib (PP242) purchased from Selleck
方法 Immunoblotting
细胞系 HeLa cells
浓度 1 uM
处理时间 2 h
结果 Treatment of cells with the MTOR catalytic inhibitors LY294002, wortmannin or PP242 induced a significant accumulation of TFEB in the nucleus in almost 100% of the cells (97.08% SD ?0.87, n = 308 for LY294002; 95.46% SD ?0.5, n = 309 for wortmannin; 92.2% SD ?1.31, n = 312 for PP242).
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Hepatology 2014, 59(4), 1262-72. LY294002 purchased from Selleck
方法 Immunoblot analysis
细胞系 Huh7 cells
浓度
处理时间 6 h
结果 Ly294002, SB203580, rottlerin, PD98059, and SP600125 were applied to Huh7 cells to specifically inhibit the activities of p38 MAPK, PKC-d, PI3K, MEK, and JNK, respectively, and the cells were subsequently stimulated with either IFN-a or IFN-b for the next 6 hours. Overall, PKC-d was found to be primarily responsible for S727 phosphorylation in the signaling pathways of both type I IFNs, with the kinase inhibitor rottlerin blocking a majority of the serine phosphorylation after 6 hours of stimulation.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Circ Res 2010, 107, 1265-1274. KU-0063794 purchased from Selleck
方法 Western blot
细胞系 SMCs
浓度 10 µmol/L
处理时间 30 min
结果 We found that mTORC2 inhibitor Ku-0063794 blocked stretch- or IGF-1-induced SGK-1 phosphorylation. However, mTORC1 inhibitor rapamycin inhibited stretch-induced phosphorylation of p70S6K, but failed to inhibit SGK-1 phosphorylation, indicating that mTORC2 is a downstream signal of IGF-1R to activate SGK-1.

Selleck产品在文献中的引用 (32)

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