96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode

Autophagy Compound Library

A unique collection of 154 autophagy signaling pathway ihibitors

规格 价格  
预溶于DMSO
100uL/well (10mM solution) RMB 38907.82
2x100uL/well (10mM solution) RMB 60587.39
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产品详情

产品描述及优势

    • A unique collection of 154 small molecules with autophagy-inducing or autophagy-inhibiting activity.
    • A useful tool for studying the roles of molecules in autophagy.
    • Targets Proteasome, HIF, HDAC, Aurora Kinase, Sirtuin, E3 Ligase, Calcium Channel, mTOR, etc.
    • Structurally diverse, medicinally active, and cell permeable.
    • Rich documentation with structure, IC50, and customer reviews.
    • NMR and HPLC validated to ensure high purity.

产品详细信息

配制: A collection of 154 autophagy signaling pathway ihibitors supplied as pre-dissolved DMSO solutions
96-孔板: 96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode
稳定性:
1年 -20°C 溶于DMSO
2年 -80°C 溶于DMSO
发货: 蓝冰物流
包装: 惰性气体

化合物库组成成份

Chemical Library Composition

客户使用Selleck产品的实验数据 (10)

AS-605240 Review
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数据来源 Nat Biotechnol ,2011, 29, 255-265. Vorinostat (SAHA, MK0683) purchased from Selleck
方法 Immunofluorescence analysis
细胞系 K562 cells
浓度 5 µM
处理时间 6 h
结果 SAHA and other nonselective HDAC inhibitors increased steady-state acetylation of tubulin and histones manifested by the staining of acetylated microtubules and punctuate nuclear staining of acetylated histone.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Nature 2014, 508(7494), 118-22. Barasertib (AZD1152-HQPA) purchased from Selleck
方法 Long-term cell proliferation assays
细胞系 A375-SOX10KD cells
浓度 0.5 uM
处理时间 4 weeks
结果 Compared with controls, treatment of A375-SOX10KD cells with a combination of both vemurafenib and GDC0941 lead to proliferation arrest.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Nature 2011, 471, 235-9. Vorinostat (SAHA, MK0683) purchased from Selleck
方法 MTT cell viability assay
细胞系 T-ALL cell lines
浓度 0.01-100 uM
处理时间 72 h
结果 It examined responsiveness to dexamethasone and the class I/II HDAC inhibitor vorinostat in a panel of T-ALL cell lines with wild type or mutant CREBBP alleles. This demonstrated sensitivity to vorinostat at clinically useful concentrations (IC50 below 1礛) in the majority of cell lines tested.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cell 2010, 142, 444–455. VX-680 (Tozasertib, MK-0457) purchased from Selleck
方法 Immunofluorescence Microscopy
细胞系 HeLa cells
浓度
处理时间
结果 Inhibition of Aurora kinases with VX-680 sharply reduced kinetochore-localized pT422 signal (Figure G). When normalized to the total level of CENP-E at the kinetochore (which is also reduced in VX-680 treated cells (Ditchfield et al. 2003)), a > 90% reduction in T422 phosphorylation was seen following VX-680 treatment ( Figure H).
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Nat Genet 2014, 46(4), 364-70. Rapamycin (Sirolimus) purchased from Selleck
方法 Western blot
细胞系 Persister cells
浓度 10 nM
处理时间 3 days
结果 While added with a specific mTOR inhibitor, rapamycin(Rapa), it inhibitied endogenous mTOR activity, showed that markedly reduced MYC protein levels in persister cells but not in naive T-ALL cells.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Nat Genet 2012, 44(8):852-60. Erlotinib HCl (OSI-744) purchased from Selleck
方法 Western blot
细胞系 HHCC827 ER1 and ER2 cells
浓度 0.1 uM
处理时间 48 h
结果 It sought to validate genetic findings using pharmacologic inhibitors of AXL. As expected, erlotinib decreased pEGFR, pERK, pAKT, pRelA and increased the levels of cleaved Parp in parental HCC827 cells irrespective of concurrent treatment with MP-470. In contrast, these effects of erlotinib treatment were observed only upon concurrent treatment with MP-470 in the HCC827 ER1 and ER2 cells.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Nat Med 2013, 19(11), 1478-88. ABT-737 purchased from Selleck
方法 Western blot
细胞系 MCF-7 cells
浓度 0/0.1/1/10 uM
处理时间 12 h
结果 Mst1 inhibition of autophagy was dose-dependently reversed by treatment with ABT-737.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Cell 2012, 21(2), 155-67. Everolimus (RAD001) purchased from Selleck
方法 Western blotting
细胞系 MP tumor cells
浓度 1, 5 uM
处理时间 3 h
结果 To confirm that these compounds were acting on the PI3K/mTOR pathway, it performed western blotting to analyze phosphorylation of critical proteins in the pathway. As shown in figure, MP tumor cells showed substantial amounts of phospho-AKT and phospho-S6 in the absence of inhibitors (DMSO lanes). RAD-001 inhibited S6 phosphorylation but did not affect phospho-AKT. In contrast with RAD-001, treatment with BEZ-235 or BKM-120 inhibited phosphorylation of both AKT and S6.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cancer Cell 2011, 19(6), 792-804. Rapamycin (Sirolimus) purchased from Selleck
方法 Cell viability Analysis
细胞系 Ar+murine (CaP8) and human (LNCaP) prostate cancer cells, Pb-Cre+;PtenL/L mice, Pb-Cre+;PtenL/L;ArL/Y mice
浓度 1 nM, 4 mg/kg
处理时间 0-4 weeks
结果 These data suggest that CaPs with AR loss have greater reliance upon the PI3K/AKT/mTOR-signaling pathways and that combined AR/androgen blockage in conjunction with PI3K/AKT/mTOR inhibition (by Rapamycin) is more effective for CaPs initiated by PTEN loss or PI3K/AKT activation.
Axitinib and PF2341066(Crizotinib) Reviews
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数据来源 Cell Stem Cell 2012, 11, 179-94. Alisertib (MLN8237) purchased from Selleck
方法 qPCR
细胞系 CCE cells
浓度 1 uM
处理时间 48 h
结果 Treatment with the Aurka-specific chemical inhibitor MLN8237 suppressed pluripotency TF expression and decreased fluorescence in the NG4 Nanog-GFP reporter line.

Selleck产品在文献中的引用 (32)

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