Phospho-CDK1/2/3(T14) Rabbit Recombinant mAb
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使用信息
| 抗体应用 | wb,ELISA | ||
|---|---|---|---|
| 稀释比例 |
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| 反应性 | Human Mouse Rat | ||
| MW (kDa) | 34kDa | ||
| 抗体类型 | Rabbit | ||
| 浓度 | 1mg/ml | ||
| 储存液配方 | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| 储存条件 (自收到货起) |
Store at –20°C. |
生物描述
| 特异性 | Phospho-CDK1/2/3(T14) Rabbit Recombinant mAb detects endogenous levels of total Phospho-CDK1/2/3(T14). |
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| 背景 | Cyclin-dependent kinases (Cdks) are regulated by positive and negative phosphorylations. T14/Y15 phosphorylation by the Wee1 and Myt1 kinases inhibits Cdks by preventing ATP binding, and these conserved phosphorylations are highly regulated during the cell cycle and by signaling pathways. Wee1 primarily phosphorylates Y15 while Myt1 is a dual-specificity kinase that can phosphorylate both sites, with a propensity toward the T14. These Cdk1 inhibitory kinases ensure that cells complete DNA replication and repair before mitosis begins. Myt1-Mediated T14 phosphorylation prevents DNA damage and genome instability. The inhibitory phosphorylations are removed by Cdc25 phosphatases. Wee1 and Cdc25 functions in mammals are best understood in G2/M. Cdk1 regulates Wee1 and Cdc25C by phosphorylation, which results in feedback loops that produce switch-like mitotic transitions. DNA damage pathways also use Cdk1 T14/Y15 phosphorylation to prevent mitotic entry by inhibiting Cdk1. Cdk2 is activated by cyclin E and cyclin A; cyclin E-Cdk2 regulates G1 progression and S-phase entry, whereas cyclin A-Cdk2 acts later in S phase and mitosis. Cdk2 is also regulated by Wee1 and Cdc25A. Cdk2 T14/Y15 phosphorylation is not required for DNA damage-induced cell cycle arrest, but instead has roles in S-phase entry and centrosome duplication. |
实验方法
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