Brefeldin A (BFA)

别名: Cyanein, Decumbin 中文名称：布雷菲德菌素A

目录号：S7046 Purity: 99.94%

Brefeldin A (BFA)作用于HCT 116细胞，抑制内酯抗生素和ATPase，作用于protein transport（蛋白转运），IC50为0.2 μM，诱导癌细胞分化和凋亡。它还能提高同源重组修复效率，是CRISPR-mediated HDR的增强剂。Brefeldin A 也是自噬和线粒体自噬的抑制剂。

Brefeldin A (BFA) Chemical Structure

CAS: 20350-15-6

规格	价格	库存
10mM (1mL in DMSO)	RMB 655.27	现货
5mg	RMB 571.63	现货
25mg	RMB 2235.02	现货
100mg	RMB 5490.64	现货
1g	RMB 28665	现货
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客户使用Selleck的Brefeldin A (BFA)发表文献67篇

产品质控

批次：纯度： 99.94%

99.94

Brefeldin A (BFA)相关产品

相关靶点	V-ATPase F-ATPase SERCA RecA AAA ATPase family	点击展开
相关化合物库	FDA药物库天然产物库离子通道配体库外泌体分泌相关化合物库钙通道阻滞剂库	点击展开

ATPase抑制剂选择性比较

细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息
HeLa	Function Assay	5 μg/ml	3 h	causes nuclear exclusion of the FoxO1 transcription factor and decreases transcription of FoxO1-regulated genes	24843827
3T3-L1	Function Assay	5 μg/ml	1 h	causes phosphorylation of the FoxO1 transcription factor	24843827
3T3-L1	Function Assay	5 μg/ml	1 h	causes redistribution of GLUT4 but not increase in glucose uptake	24843827
3T3-L1	Function Assay	5 μg/ml	30 min	causes reversible redistribution of GLUT4	24843827
3T3-L1	Function Assay	5 μg/ml	30 min	recapitulates insulin action with respect to regulating Akt activity and AS160 phosphorylation	24843827
3T3-L1	Function Assay	5 μg/ml	30 min	mimics the effects of insulin and causes robust phosphorylation of Akt (Ser 473) and phosphorylation of AS160 (Thr 642 and Ser 588)	24843827
H838-KDLKB1	Function Assay	30 ng/ml	12/18 h	increases the levels of phosphorylated eIF2α (phospho-eIF2α)	25011082
H838-KDLKB1	Function Assay	30 ng/ml	12/18 h	increases the protein levels of BiP	25011082
H838-LKB1	Function Assay	30 ng/ml	12/18 h	increases the protein levels of BiP	25011082
HepG2	Function Assay	1μg/mL	3–24 h	increases the level of APE1 in a time-dependent manner	25026174
Huh-7	Function Assay	1μg/mL	3–24 h	increases the level of APE1 in a time-dependent manner	25026174
RBE4	Function Assay	2 μM	3–24 h	induces an overload of Ca2+ in the mitochondria in the first 6 h of incubation (p < 0.001) but Ca2+ levels in this organelle decreased after 12 h of incubation	25128025
RBE4	Function Assay	2 μM	3–24 h	induces a delayed depletion of the ER Ca2+ content at 6 h of incubation significantly	25128025
RBE4	Function Assay	2 μM	3–24 h	increases the levels of ROS time-dependently	25128025
RBE4	Function Assay	2 μM	3–24 h	increases active caspase-12 in a time-dependent manner	25128025
RBE4	Function Assay	2 μM	3–24 h	increases the XBP1 protein levels after 3 and 6 h of treatment	25128025
RBE4	Apoptosis Assay	2 μM	3–24 h	induces apoptosis time dependently	25128025
HEK293/hERG	Function Assay	10 μM	1 h	results in a time-dependent reduction mature hERG protein	25218469
MEC	Function Assay	1 μM	1.5 h	causes a dramatic decrease in the surface VEGFR2	25228815
KMS-6	Function Assay	1 μM	24 h	exhibits half the secretion of galanin-LI as did the control	25229126
A172	Function Assay	10 μg/ml	4 h	results in the retrograde transport of fluorescent granules	25239507
MDA-MB-231	Function Assay	0–50 μg/mL	24 h	inhibits the formation of 3D and 2D colonies	25356567
MDA-MB-231	Apoptosis Assay	0.05–1 μg/mL	24 h	induces PARP (poly ADP-ribose polymerase-1) cleavage	25356567
MDA-MB-231	Growth Inhibition Assay	0.01/0.05 μg/mL	24 h	increases the fraction of sub-G1 cell debris	25356567
MDA-MB-231	Apoptosis Assay	0.1 μg/mL	4 h	induces apoptosis	25356567
MDA-MB-231	Cell Viability Assay	0–50 μg/mL	48 h	EC50 = 0.016 µg/mL	25356567
H1299	Function Assay	10 μg/ml	24 h	induces autophagy	25388970
PEXEL-Nluc	Function Assay	5 mg/mL	6 h	causes an increase in reporter activity in the parasite	25392998
SP-Nluc	Function Assay	5 mg/mL	6 h	causes an increase in reporter activity in the parasite	25392998
iPSC-CMs	Function Assay	500 ng/ml	48 h	increases the intensity of the higher mobility LAMPs at the cost of the lower mobility species	25488666
OB-6	Apoptosis Assay	2.7 μM	48 h	induces apoptosis	25532480
SMCs	Function Assay	1μg/mL	3 h	accumulates CNPY2 protein in the ER compartment and no longer co-localized with the Golgi marker	25589425
HepG2	Function Assay	1 µM	24 h	decreases the level of PXR mRNA	25616597
FRT	Function Assay	5 μg/ml	2 h	prevents the increase in cleaved α subunits when [Na+]i was reduced	25767115
FRT	Function Assay	5 μg/ml	2 h	blocks trafficking through the Golgi complex by inhibiting ER-to-Golgi transport	25767115
nHDFs	Function Assay	1 μM	2 h	prevents the assembly of cytosolic coat proteins onto Golgi membranes	25772616
DF1	Function Assay	1 μM	48 h	disperses the exogenous CSGalNAcT2 protein	25807054
COS	Function Assay	1 μg/ml	3 h	completely disperses the AP-1 signal	25915900
HeLa	Function Assay	200 ng/ml	3 h	induces the artificial break-up of the Golgi complex	25948586
NRK	Function Assay	200 ng/ml	4 h	rescues mitotic progression	25948586
Caco-2	Function Assay	2.5 μM	30 min	attenuates the TGF-β1-mediated increase in SERT function	25954931
HUVEC	Function Assay	10 μM	1 h	increases the number and intensity of fluorescent areas especially in perinuclear space	25956988
HUVEC	Function Assay	10 μM	1 h	abolishes hypoxia-induced release of ATP from apical and basolateral surfaces	25956988
HEMC-1	Function Assay	0.1 µg/ml	24 h	causes a higher inhibitory effect on exocytosis than nocodazole	25972759
SMCs	Function Assay	10 µg/ml	0-12 h	causes a transient Ca2+ release from the ER/SR	26172080
SMCs	Function Assay	10 µg/ml	0-12 h	shows a trend towards a higher concentration of the ER/SR network in the perinuclear area	26172080
MEFs VAMP7 KO	Function Assay	5 μM	20 min	causes resident enzymes such as NAGT-GFP, to diffuse back to the ER	26196023
MEFs WT	Function Assay	5 μM	20 min	causes resident enzymes such as NAGT-GFP, to diffuse back to the ER	26196023
C2C12	Function Assay	1 μg/ml	1 h	abolishes cytokine release from C2C12 myotubes	26291279
PC12	Function Assay	2 μM	1 h	inhibits the L-DOPA (20 μM)-induced transient ERK1/2 phosphorylation	26363191
HEK293	Function Assay	5 μg/ml	12 h	abolishes CMA-induced CRELD2 secretion	24687431
COS-1	Function Assay	5 µg/ml	24 h	restricts localization of NB in the perinuclear region	24671751
RAW264.7	Apoptosis Assay	4 μM	48 h	attenuates the inhibition of ox-LDL-induced apoptosis and the facilitation of cholesterol efflux by Ac-hE-18A-NH2	24639032
MDMs	Apoptosis Assay	10 μg/ml	12/15 h	induces apoptosis	24556695
PMHs	Function Assay	10–20 μg/ml	24 h	induced ER stress	24407242
PMHs	Apoptosis Assay	10–20 μg/ml	24 h	increases cell death	24407242
HEK293/tau	Function Assay	5 μM	1/2/4 h	induces Golgi fragmentation	24368089
HEK293/tau	Function Assay	5 μM	3 h	induces tau hyperphosphorylation	24368089
ADF	Function Assay	10 μM	16 h	inhibits the ZnCl2-induced translocation of CRT	24228232
U373	Function Assay	10 μM	16 h	inhibits the ZnCl2-induced translocation of CRT	24228232
RKO-HIPK2i	Function Assay	10 μM	16 h	inhibits the ZnCl2-induced translocation of CRT	24228232
ADF	Function Assay	10 μM	6 h	impairs the DC activation	24228232
Huh7	Function Assay	5 μg/ml	4 h	abolishes the secretion of intracellular ApoB	24100140
Huh7	Function Assay	5 μg/ml	1 h	causes a significant increase in ApoB-crescents	24100140
Huh7	Function Assay	5–10 ng/ml	12 h	increases ApoB-crescents without inhibiting secretion	24100140
BAECs	Function Assay	5 μg/ml	0-4 h	induces the rapid dephosphorylation of eNOS at Ser1179	24085225
Macrophages	Function Assay	71 µM	6 h	inhibits lunasin internalization	24039740
Colo 205	Growth Inhibition Assay	0-5 μg/mL	48 h	inhibits cell growth in suspension cultures with an estimated IC50 of ~15 ng/mL	23973996
Colo 205	Function Assay	0.012-0.025 μg/mL	14 d	reduces the clonogenicity of Colo 205 CSCs	23973996
Colo 205	Apoptosis Assay	0.1 μg/mL	0-24 h	induces apoptosis of Colo 205 cells in suspension cultures	23973996
Colo 205	Function Assay	0.015 μg/mL	24 h	induces the expression of ER stress-related genes	23973996
Colo 205	Function Assay	0.015 μg/mL	24 h	inhibits the activity of MMPs	23973996
IBRS2	Function Assay	5 μg/ml	0.5 h	disrupts the ERGIC and Golgi	23963534
IBRS2	Function Assay	5 μg/ml	0.5 h	enhances FMDV infection	23963534
HeLa	Function Assay	2 μM	2 h	attenuates the TNF-induced secretion of IL-15	23950892
HFS	Function Assay	0-1 μg/ml	24 h	GLTP expression reaches a plateau at concentrations as low as 0.01 µg/ml	23894633
HFS	Function Assay	0.01 µg/ml	24 h	increases the expression of glycosphingolipid synthase genes at 6 h	23894633
OVCAR-3	Growth Inhibition Assay	1–15 μM	24 h	induces a loss of cell viability dose dependently	23826964
OVCAR-3	Function Assay	1–15 μM	24 h	induces nuclear damage	23826964
OVCAR-3	Apoptosis Assay	1-10 μM	4 h	induces the activation of apoptosis-related proteins	23826964
OVCAR-3	Apoptosis Assay	10 μM	24 h	induces activation of caspases	23826964
OVCAR-3	Function Assay	1–10 μM	24 h	induces disruption of the mitochondrial transmembrane potential	23826964
OVCAR-3	Function Assay	1–10 μM	24 h	induces formation of reactive oxygen species	23826964
OVCAR-3	Function Assay	1–10 μM	24 h	inhibits cell adhesion and migration	23826964
HeLa	Function assay	100 uM	2 hrs	Dispersion of cis golgi marker betaCoP in human HeLa cells at 100 uM for 2 hrs	17563369
HeLa	Function assay	100 uM	2 hrs	Dispersion of cis golgi marker KDEL in human HeLa cells at 100 uM for 2 hrs	17563369
Vero	Function assay	10 ug/ml	5 mins	Inhibition of Arf1 in african green monkey Vero cells assessed as rapid AP-1 dispersal from golgi membranes at 10 ug/ml after 5 mins by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	5 mins	Inhibition of Arf1 in african green monkey Vero cells assessed as rapid GGA3 dispersal from trans golgi network at 10 ug/ml after 5 mins by immunofluorescence method	19182783
Vero	Function assay	10 uM	1 hr	Inhibition of GBF1 QNV deleted mutant in african green monkey Vero cells assessed as effect on change in golgi morphology at 10 uM after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 uM	1 hr	Inhibition of GBF1 QNV to AAA mutant in african green monkey Vero cells assessed as effect on change in golgi morphology at 10 uM after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	1 hr	Inhibition of Arf1 in african green monkey Vero cells assessed as decrease in Arf1-GTP levels at 10 ug/ml after 1 hr	19182783
Vero	Function assay	10 uM	1 hr	Inhibition of GBF1 in african green monkey Vero cells assessed as punctate and diffuse distribution of medial-Golgi marker giantin from TGN at 10 uM after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	1 hr	Inhibition of Arf1 in african green monkey Vero cells assessed as punctate and diffuse distribution of medial-Golgi marker giantin at 10 ug/ml after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	5 mins	Inhibition of Arf1 in african green monkey Vero cells assessed as rapid COPI redistribution from golgi at 10 ug/ml after 5 mins by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	1 hr	Inhibition of Arf1 in african green monkey Vero cells assessed as tubule formation from trans golgi network and endosomes before its dispersal at 10 ug/ml after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	1 hr	Inhibition of Arf1 in african green monkey Vero cells assessed as giantin positive punctate structures in contact with Sec31-positive ER exit site at 10 ug/ml after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 uM	1 hr	Induction of GBF1 in african green monkey Vero cells assessed as punctate and diffuse distribution of cis-Golgi marker GM130 from TGN at 10 uM after 1 hr by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml	1 hr	Inhibition of Arf1 in african green monkey Vero cells assessed as punctate and diffuse distribution of cis-Golgi marker GM130 at 10 ug/ml after 1 hr by immunofluorescence method	19182783
NRK	Function assay	7 uM	60 mins	Golgi-disturbing activity in golgi apparatus of rat NRK cells assessed as fusion of golgi membrane fusion with endoplasmic reticulum at 7 uM after 60 mins by Hoechst 3342 staining-based immunofluorescence microscopy	20189813
HeLa R19	Antiviral assay	0.5 uM	7 hrs	Antiviral activity against Coxsackievirus B3 infected in human HeLa R19 cells assessed as inhibition of viral replication at 0.5 uM after 7 hrs by luciferase reporter gene assay	23805957
HeLa	Function assay	5 uM	30 to 60 mins	Induction of golgi apparatus disassembly in human HeLa cells at 5 uM after 30 to 60 mins by confocal microscopic analysis	23805957
Arabidopsis thaliana root cells	Function assay	90 uM	30 mins	Induction of morphological changes of golgi apparatus in Arabidopsis thaliana root cells expressing ST-YFP/VHAa1-RFP at 90 uM after 30 mins by confocal laser scanning microscopic analysis	23805957
HeLa R19	Antiviral assay	5 to 50 uM	7 hrs	Antiviral activity against Coxsackievirus B3 infected in human HeLa R19 cells assessed as inhibition of viral replication at 5 to 50 uM after 7 hrs by luciferase reporter gene assay	23805957
PC3	Function assay	50 nM	72 hrs	Potentiation of 3 nM docetaxel-induced cytotoxicity against human PC3 cells assessed as decrease in cell viability at 50 nM after 72 hrs by trypan blue exclusion assay	28462831
HeLa	Function assay	18 uM	3 hrs	Inhibition of alkaline phosphatase secretion in human HeLa cells at 18 uM incubated for 3 hrs	31421965
PRP	Function Assay	10 μM		abrogates SDF-1α-mediated CXCR7 externalization	24668750
Vero	Function assay	10 uM		Inhibition of GBF1 in african green monkey Vero cells assessed as inhibition of StxB-SS retrogade transport from endosomes to TGN at 10 uM by immunofluorescence method	19182783
Vero	Function assay	10 ug/ml		Inhibition of Arf1 in african green monkey Vero cells assessed as inhibition of StxB-SS retrogade transport from endosomes to TGN at 10 ug/ml by immunofluorescence method	19182783
L02	Cytotoxicity assay		72 hrs	Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50<0.0004μM.	28494251
PC3	Antiproliferative assay		72 hrs	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay, IC50=0.068μM.	28494251
HT-29	Antiproliferative assay		72 hrs	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay, IC50=0.16μM.	28494251
HepG2	Antiproliferative assay		72 hrs	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay, IC50=0.35μM.	28494251
LO2	Antiproliferative assay		72 hrs	Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay, IC50<0.001μM.	29524728
Bel7402	Antiproliferative assay		72 hrs	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay, IC50=0.024μM.	29524728
HL60	Antiproliferative assay		72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, IC50=0.025μM.	29524728
PC3	Antiproliferative assay		72 hrs	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay, IC50=0.068μM.	29524728
Bel7402/5-FU	Antiproliferative assay		72 hrs	Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay, IC50=0.82μM.	29524728
VERO-E6	Function assay		48 hrs	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging, IC50=0.02μM.	ChEMBL
VERO-E6	Function assay		48 hrs	Toxicity CC50 against VERO-E6 cells determined at 48 hours by high content imaging (same conditions as 2_LEY without exposure to 0.01 MOI SARS CoV-2 virus), CC50=0.06μM.	ChEMBL
MKN45	Growth Inhibition Assay			IC50<0.001 μg/ml	23793342
LOVO	Growth Inhibition Assay			IC50=0.12 μg/ml	23793342
A549	Growth Inhibition Assay			IC50=0.04 μg/ml	23793342
MDA-MB-435	Growth Inhibition Assay			IC50<0.001 μg/ml	23793342
HepG2	Growth Inhibition Assay			IC50<0.001 μg/ml	23793342
HL-60	Growth Inhibition Assay			IC50<0.001 μg/ml	23793342
neural precursor cells	Function assay			Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay	17417631
NCI60	Cytostatic assay			Cytostatic activity against human NCI60 cells by SRB assay, GI50=0.0206μM.	23805957
NCI60	Cytostatic assay			Cytostatic activity against human NCI60 cells by SRB assay, TGI=3.48μM.	23805957
点击查看更多细胞系数据

生物活性

产品描述

靶点

ATPase (HCT 116) ^[1]

0.2 μM

体外研究(In Vitro)
体外研究活性	Brefeldin A是一种真菌代谢产物，抑制内质网和高尔基体之间的传输，Brefeldin A导致膜蛋白分布受损。Brefeldin A处理HCT 116人结肠癌细胞，观察到形态学的变化，表示细胞分化。Brefeldin A作用于肿瘤细胞，主要通过诱导分化和凋亡而发挥其细胞毒性作用。^[1^] 20 μg/mL Brefeldin A处理检测条6小时，在10mM Indomethacin 和 30 μM L-NOARG存在时，完全废除Bradykinin诱导的松弛。浓度为1 nM 到 1 mM之间的 20 μg/mL Brefeldin A处理，废除Bradykinin诱导的[Ca²⁺]_i降低。Brefeldin A作用于内皮细胞，对Bradykinin 或 Substance P诱导的[Ca²⁺]_i 提高没有影响。^[2] Brefeldin A 不影响GTPS与myr-rARF1的自发磷脂依赖性的结合，但完全废除视网膜等渗提取物（RIE）的催化交换，2 μM Brefeldin A的半抑制浓度为2 μM。Brefeldin A 抑制多种膜转运途径。Brefeldin A作用于高尔基体膜或脑细胞质，抑制ADP-核糖基化因子特定的鸟嘌呤核苷酸交换活性。Brefeldin A完全抑制说明视网膜提取物包含ARF特定的鸟嘌呤核苷酸交换因子。Brefeldin A只部分抑制ADP-核糖基化因子（ARFs）释放的视网膜等渗提取物（RIE）催化的GTPS，即使浓度高达300 μM时。^[3] Brefeldin A诱导高尔基体与ER融合。Brefeldin A废除 CERT 抑制剂HPA-12的抑制效果。Brefeldin A处理CHO细胞，使鞘磷脂的合成提高2到3倍。^[4] 除了B-CLL细胞，Brefeldin A还造成多发性骨髓瘤（U266，NCI-H929），JURKAT，HeLa细胞，白血病（HL60，K562，BJAB），结肠（HT-29），和前列腺，及腺样囊性瘤细胞发生凋亡。25 ng/mL Brefeldin A处理 HF4.9 和 HF28RA 细胞，完全抑制细胞生长，而要完全抑制HF1A3 细胞生长则需要高剂量的Brefeldin A (75 ng/mL) 。50-75 ng/mL Brefeldin A 处理HF1A3, HF4.9 和 HF28RA细胞，24小时内抑制细胞增殖，这种作用存在剂量依赖性，³H-胸甘的渗透几乎完全停止，50 ng/ml处理时，HF1A3, HF4.9 和 HF28RA细胞分别抑制26%, 76%, 87%，75 ng/mL处理时，HF1A3, HF4.9 和 HF28RA细胞分别抑制75%, 87%, 92%。YO-PRO 1/PI检测中，Brefeldin A诱导细胞死亡，这种作用存在剂量依赖性。^[5] Brefeldin A可提高同源重组效率，是CRISPR-mediated HDR的增强剂^[5]。
细胞实验	细胞系	人类滤泡性淋巴瘤细胞系HF1A3，HF4.9 和HF28RA
	浓度	0 ng/mL-75 ng/mL
	孵育时间	5 天
	方法	使用 YO-PRO 1/PI 和 SYTO16/PI 探针对细胞进行双染色，测定HF1A3, HF4.9细胞活力。为了测定细胞增殖使用0–100 ng/mL Brefeldin A 在完全培养基中处理细胞20小时，然后加入1 μCi/mL [methyl-³H]-胸甘在37°C下再处理4小时。使用 Microbeta 计数仪对渗透的放射性胸甘进行闪烁计数。为了测定Brefeldin A治疗的长期效果，细胞按初始浓度10⁵ cells/mL接种，然后使用0-75 ng/mL Brefeldin A处理长达5天。在指定时间，移除细胞样本，通过标准台酚蓝排除法测定存活细胞数。
实验图片	检测方法	检测指标	实验图片	PMID
	Western blot	p53 / GRP78		22859938
	Immunofluorescence	FMNL1 / GM130 ErbB3 / Calnexin MTP / GBF1		21868368
	Growth inhibition assay	Cell viability		28462831

参考文献
[1] Zhu JW, et al. Bioorg Med Chem. 2000, 8(2), 455-463. [2] Ohnishi Y, et al. Br J Pharmacol. 2001, 134(1), 168-178. [3] Franco M, et al. J Biol Chem. 1996, 271(3), 1573-1578. [4] Hullin-Matsuda F, et al. J Biol Chem. 2012. [5] Wlodkowic D, et al. Leuk Res. 2007, 31(12), 1687-1700. [6] Chen Yu, et al. Cell Stem Cell. 2015, 16(2): 142-147. [7] Persa E, et al. Int J Mol Sci. 2018 Aug 14;19(8):2391. + 展开

参考文献

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化学信息&溶解度

分子量	280.36	分子式	C₁₆H₂₄O₄
CAS号	20350-15-6	SDF	Download Brefeldin A (BFA) SDF
Smiles	CC1CCCC=CC2CC(CC2C(C=CC(=O)O1)O)O
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 56 mg/mL ( (199.74 mM)； DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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