MK-2206 2HCl

目录号:S1078

MK-2206 2HCl Chemical Structure

Molecular Weight(MW): 480.39

MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂,在无细胞试验中IC50分别为8 nM/12 nM/65 nM;对250种其他蛋白激酶没有抑制活性。Phase 2。

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RMB 1789.01 现货
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客户购买Selleck的此次产品后发表的文献180篇:

客户使用该产品的19个实验数据:

  • Inhibition of AKT signaling abolishes MKK4 phosphorylation on Ser78 in injured axons. Cultures of sensory neurons were treated with 5 µM MK-2206 or 5 µM GDC-0068 for 1 hr prior to axotomy. Axonal proteins harvested at indicated time points after axotomy were subjected to immunoblot analysis.

    Cell, 2015, 160(1-2): 161-76 . MK-2206 2HCl purchased from Selleck.

    Cancer Cell 2013 24, 766-76. MK-2206 2HCl purchased from Selleck.

  • The PI3K or AKT inhibition does not restore sensitivity to WZ4002 in PC9 WZR. PC9GR4 or WZR10 cells were treated with WZ4002 alone at the indicated concentrations or in combination with the AKT inhibitor MK-2206 (1 uM). MK-2206 effectively inhibited AKT in both cells.

    Cancer Discov 2012 2, 934-47. MK-2206 2HCl purchased from Selleck.

    Rap1b negatively regulates neutrophil transcellular migration by limiting PI3K-Akt signaling. (A-D) Effect of Akt inhibitor MK2206 (2 uM), Src inhibitor PP2 (10 uM), or vehicle control (DMSO) on WT or Rap1b-/- neutrophil functions. (A) Percentage of neutrophil transendothelial migration in 3D migration model. (B) ECM degradation assessed on Oregon green-labeled gelatin matrix; (left) representative images on (bar, 10 um) and (right) bar graph is percentage of matrix degradation. (C) Percentage of cells forming multiple protrusions. (D) Percentage of neutrophils present at junction of activated bEND.3 in 3D migration assay. Mean ?SD; n = 3 independent experiments. **, P < 0.01; ***, P < 0.001; NS, not significant using unpaired Student's t test).

    J Exp Med 2014 211(9), 1741-58. MK-2206 2HCl purchased from Selleck.

  • VE-cadherin-induced Akt activation mediates YAP phosphorylation and translocation in ECs. HUVECs were starved for 1h and treated with thrombin (1U) for 1h. Total cell lysates were probed with anti-pAkt, Akt or b-actin antibody. The representative blots of three independent experiments are depicted, and the normalized values for p-Akt are shown. HUVECs were cultured and starved as described as in d and incubated for 8h in complete medium with the Akt inhibitor, MK-2206 (1 uM). pAkt, Akt, pYAP and YAP were detected by western blotting using specific antibodies.

    Nat Commun 2015 6:6943. MK-2206 2HCl purchased from Selleck.

    Inhibitors of AKT or ERK overcome SDF-1a-mediated resistance to ibrutinib-triggered PARP and caspase 3 cleavage in CXCR4S338X-expressing BCWM.1 cells. CXCR4S338X-expressing WM cells were treated with ibrutinib (0.5 uM) alone or in the presence of SDF-1a (20 nM) and/or the AKT inhibitors MK-2206 (0.5 uM) and AZD-5363 (0.5 uM); or the MEK inhibitors AS-703026 (0.25 uM), AZD-6244 (0.5 uM) and UO126 (5.0 uM). (a) Immunoblotting results for phosphoAKT (S473) and phospho-ERK (T202/Y204) in CXCR4S338X-expressing BCWM.1 cells pretreated with ibrutinib with and without AKT or ERK inhibitors, then subjected to SDF-1a stimulation for 2 min. The inhibitory effect of AZD-5363 on AKT, which is known to paradoxically hyper-phosphorylate pAKT(S473) was confirmed by inhibition of the phospho-activity for the downstream AKT targets glycogen synthase kinase 3b and pS6. (b) Immunoblotting results for cleaved PARP and cleaved caspase 3 in CXCR4S338X-expressing BCWM.1 cells treated with ibrutinib and/or AKT or ERK inhibitors for 6 h at IC50 doses. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Leukemia 2015 29(1), 169-76. MK-2206 2HCl purchased from Selleck.

  • Induction of apoptosis positively correlates with FOXO3a protein levels and phosphorylation. (a) Treatment of 11 cell lines seeded in 6-well plates with 8 μM MK-2206 or DMSO for 48 hours. Nuclei were harvested for PI staining and FACS analysis and the differences between drug and no drug are displayed as in Figure b. Both conditions were performed in triplo for each cell line. Below the FACS data is a we stern blot of cell lysates from this panel treated with 8 μM MK-2206 or DMSO and harvested at 24 hours. Each cell line name corresponds to the two western lanes below it and the four cell cycle bars above it.

    Cancer Res 2013 73, 2189-98. MK-2206 2HCl purchased from Selleck.

    IC50 values for an Akt inhibitor (MK-2206) and a MEK inhibitor (U0126) in a panel of mouse and human anaplastic (ATC) and follicular (FTC) carcinoma cell lines. On the bottom, a Western blotting showing the effect on the activation of Akt and ERK1/2 of one hour exposure to these inhibitors (MK-2206: 500 nM, U0126: 10 μM).

    Oncotarget 2011 2, 1109-26. MK-2206 2HCl purchased from Selleck.

  • Mean IL-8 concentrations determined by ELISA of the supernatants of HeLa cells infected with wild-type Salmonella. Kinase inhibitors are indicated on the x axis, and the target families of the inhibitors are indicated above each column. CEC, chelerythrine; Pim Inh, Pim-1 inhibitor 2. Inhibitors that significantly affected IL-8 production relative to the control (P < 0.05, Bonferroni post hoc test from one-way ANOVA) are indicated with an asterisk. Relative cell viability is also shown, as determined by reduction of XTT by viable cells. A450, absorbance at 450 nm.

    Sci Signal 2011 4, rs9. MK-2206 2HCl purchased from Selleck.

    Reducing cellular levels of PtdIns5P by overexpression of PIP4Kα inhibits clonogenic growth of U2OS but enhances cell survival in response to H2O2 stimulation. A) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), PI-103 (0.5 μM), U0126 (5 μM), or SB203580 (5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown. B) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), MK-2206 (1 μM), triciribine (1 μM), KU0063794 (1 μM), or PI-103 (0.5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown.

    FASEB J 2013 27, 1644-56. MK-2206 2HCl purchased from Selleck.

  • A. Western blot analysis of pAKT activation in ovarian cancer cell lines treated with exogenous IGF-1. B. Activation of pAKT by IGF-1 in low-grade ovarian cancer cell lines was blocked by the AKT inhibitor MK-2206 in a dose-dependent manner.

    Gynecol Oncol 2011 123, 13-8. MK-2206 2HCl purchased from Selleck.

    Confocal microscopy images of NO formation. DAF 2 DA-loaded washed platelets (1.0109 platelets/mL) were preincubated at 378C with saline (A), and then stimulated for 1min with 0.1 (B), 1.0 (C) or 10 (D) μM AEA. In Panel (E-F-G) washed platelets were preincubated with 1 μM SR1 (E), 1 Mm MK2206 (F) or 20 μM LY294002 (G), and then stimulated for 1min with 1.0 μM AEA. In panel (H) is reported the effect of 5 mg/mL collagen, used as a positive control. All the experiments were carried out in the presence of 100 μM L-arginine. NO formation was visualized by confocal microscropy as detailed in Methods.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

  • The AEA effect on eNOS phosphorylation. Washed platelets (1.0109 platelets/mL), preincubated at 378C with saline, 1 μM SR1, 1 μM SR2, 20 μM LY294002, 1 μM MK2206, 1.0 μM EGTA, or 30 μM BAPTA/AM, were stimulated for 1 min with 1.0 μM AEA. At the end of incubation suitable aliquots were immunoblotted with anti-p-eNOSser1177 as detailed in Methods.Blots are representative of five independent experiments.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

    Effect of selected agents on NOx and cGMP formation induced by AEA. Washed platelets (1.0109 platelets/mL), prewarmed at 378C with saline or 1mM SR1, 1 μM SR2, 20 μM URB597 (URB), 1 μM MK2206 (MK) or 20 μM LY294002 (LY), were incubated for 1min with 100 μM L-arginine in the presence of 1.0 μM AEA. NOx (panel A) and cGMP (panel B) content were determined as detailed in Methods. Each bar represents the meanSD of five independent experiments carried out in triplicate. Student,s t-test: P <0.0001 versus none;P <0.0005; P <0.005 versus AEA

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

  • C.U87MG cells were treated with vehicle or 1 μM MK-2206 for 1 hr, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control.

    D. Cells were treated with vehicle (control) or 1 μM MK-2206 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.
     

     

    Radiat Oncol 2009 4, 43. MK-2206 2HCl purchased from Selleck.

    MK-2206 2HCL inhibited the sorafenib-induced PI3K/mTOR pathway activation and enhanced the cytotoxic effects of sorafenib in resistant cell lines. (A) HUH-7 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved PARP were revealed by Western blotting. (B) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved RARP were revealed by Western blotting. (C) HUH-7 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (D) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (*P < 0.05, HUH-7, SK-HEP-1 are control groups, R-HUH-7, R-SK-HEP-1 are resistant groups).

    J Surg Res, 2016, 206(2):371-379. MK-2206 2HCl purchased from Selleck.

  • After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of MK-2206 for 3h,followed by 15-minute stimolation of 100ng/ml EGF.

     
     

     

    Dr. Zhang of Tianjin Medical University. MK-2206 2HCl purchased from Selleck.

    Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823, 987-96. MK-2206 2HCl purchased from Selleck.

  •  

    PI3K pathway signaling in GDC-0941 and MK-2206 treated MCF-7 derivatives with PIK3CA or  AKT1 mutations. Cells were grown in medium containing 5% FBS and treated with vehicle or increasing concentrations of GDC-0941 (0 nM, 50 nM, 100 nM, and 400 nM) or MK-2206 (0 nM, 100 nM, 250 nM, 1000 nM). After 24 hours of drug treatment, lysates were prepared and equal amounts of protein were load ed onto SDS-PAGE gels and blotted with the indicated antibodies.

    MK-2206 2HCl purchased from Selleck.

产品安全说明书

Akt抑制剂选择性比较

生物活性

产品描述 MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂,在无细胞试验中IC50分别为8 nM/12 nM/65 nM;对250种其他蛋白激酶没有抑制活性。Phase 2。
特性 MK-2206是第一个进入临床研究阶段的Akt小分子变构抑制剂。
靶点
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
体外研究

MK-2206是变构抑制剂,由pleckstrin同源结构域激活。MK-2206抑制Akt的苏氨酸308位点和丝氨酸473位点的自身磷酸化作用。另外,MK-2206阻止Akt调节的下游信号分子(包括TSC2, PRAS40,及核糖体S6蛋白)的磷酸化作用。与抑制Ras 突变型细胞系(如NCI-H358, NCI-H23, NCI-H1299, 和Calu-6)相比,MK-2206 更有效地抑制Ras野生型细胞系(如A431, HCC827, 和NCI-H292)。MK-2206和细胞毒素药剂如erlotinib 和lapatinib联用作用于肺部NCI-H460肿瘤细胞或者卵巢A2780肿瘤细胞,MK-2206也显示出协同效应。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H292 NYjkTFcxS3m2b4TvfIlkKEG|c3H5 MkL2N{DPxE1? MXe3NkBp MVzEUXNQ MoG3TY5pcWKrdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25? NIDRSW4zODV5MUC2PS=>
A431 NIPkNmRMcW6jc3WgRZN{[Xl? MXO1JO69VQ>? M{Lye|UhcA>? MnPvSG1UVw>? M{XWWXN2eHC{ZYPz[ZMhfGinIIPp[45idGmwZzDv[kBCc3RiYX7kJGVzcw>? NVTkW4tjOjB3N{GwOlk>
HepG2 Ml\pR5l1d3SxeHnjJGF{e2G7 Ml\BNVAh|ryP NYnWNJZxOjRiaB?= NHTFO|ZFVVOR M4fIe3NmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> MoC3NlEzODV7MkW=
Sk-Hep1 NInGSlNEgXSxdH;4bYMhSXO|YYm= NWTTN2tmOTBizszN MUCyOEBp MmHZSG1UVw>? MoPuV4Vve2m2aYrld{Bz\XOrc4ThcpQh[2WubIOgeI8hfGinIHP5eI91d3irYzDl[oZm[3Rib3[gd49z[W[nbnni NHfPb2ozOTJyNUmyOS=>
OCUT1 cells harbored PIK3CA (H1047R+/+) NYi4OIo1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{W0UlMh|ryP NIntdmg2KGR? MlTRSG1UVw>? NXuyOHdUUUN3ME2wMlE1KM7:TR?= NH;2ZmQzOTJ6OUK2Oy=>
K1 cells harbored PIK3CA (E542K+/+) NIXvb45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fTOFMh|ryP NFLtU4g2KGR? MWjEUXNQ NUnt[HdEUUN3ME2wMlUzKM7:TR?= MXiyNVI5QTJ4Nx?=
FTC133 cells harbored PTEN (allele deletion and R130+) NWjwUnRlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrLV|Q{KM7:TR?= MXe1JIQ> NGLXdYZFVVOR MWjJR|UxRTBwMUig{txO NYfOPWZMOjF{OEmyOlc>
C643 cells harbored HRAS (G13R+/−) NYTiSlJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWmzJO69VQ>? Mm\POUBl NFr5SGtFVVOR NGKzXHFKSzVyPUCuNlch|ryP NYHHOmxWOjF{OEmyOlc>
Hth7 cells harbored NRAS (Q61R+/−) M{W5Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXyzJO69VQ>? MVi1JIQ> MnjSSG1UVw>? NWi5UZgyUUN3ME20MlUh|ryP MWeyNVI5QTJ4Nx?=
TPC1 cells harbored RET/PTC1 rearrangement MkjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWOzJO69VQ>? M{\aW|Uh\A>? MWTEUXNQ M4TrXWlEPTB;MD61PUDPxE1? M4HuUlIyOjh7Mk[3
Hth74 NF70eGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\XN{DPxE1? MWq1JIQ> Ml\ESG1UVw>? NFnxeHJKSzVyPUKuNVkh|ryP NVz1VFdYOjF{OEmyOlc>
KAT18 NVjWOVFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml2wN{DPxE1? NIPU[pE2KGR? MoS0SG1UVw>? MXzJR|UxRTRwNkKg{txO M{TZNFIyOjh7Mk[3
SW1736 NGDpPXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1H2O|ExOCEQvF2= Mo\1OUBl NFjiemhFVVOR NGfDbm9KSzVyPUS3MlU3KM7:TR?= NXvtd5dxOjF{OEmyOlc>
WRO NWPlO2R6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfwT2dbOTByMDFOwG0> NXm4[Hd5PSCm MYDEUXNQ NGXIR2pKSzVyPkGwNFAh|ryP MVKyNVI5QTJ4Nx?=
TAD2 M1nXdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHq2NoUyODByIN88US=> MnjqOUBl MVPEUXNQ M1i4[mlEPTB-MUCwNEDPxE1? MWWyNVI5QTJ4Nx?=
LN229 M4jjXWFxd3C2b4Ppd{BCe3OjeR?= Mk\aNE42KM7:TR?= NVHZ[5lWPjBiaB?= M4HZZmROW09? MXrBeYdu\W62czDhdI9xfG:pZX7pZ{Bm\m[nY4TzJI9nKGenZnn0bY5q[g>? MmjnNlIxPTd7MUS=
T98G M2nq[mFxd3C2b4Ppd{BCe3OjeR?= MV:wMlUh|ryP MVm2NEBp M4PEe2ROW09? NGrGbWlCfWevZX70d{BieG:ydH;n[Y5q[yCnZn\lZ5R{KG:oIHfl[ol1cW6rYh?= NGC1SWUzOjB3N{mxOC=>
HC11 MYfGeY5kfGmxbjDBd5NigQ>? MUexNEDPxE1? NHTWfGQzPCCq NHfvSG5FVVOR NWSxOJB5UW6qaXLpeJMh|rJvY3Hz[YlvKGGwZDDBSHJRKHO7boTo[ZNqew>? NX3nWnNWOjJ2Mk[2NlE>
MOLT-4 MX\DfZRwfG:6aXOgRZN{[Xl? NFj2U5kyOCEQvF2= MUi0PEBp NI\5TJRFVVOR NE\UOlFKSzVyPUGuO-KBkc7:TR?= MWWyNlYyPDJ2Mx?=
CEM-R MonYR5l1d3SxeHnjJGF{e2G7 NHvxRW4yOCEQvF2= MVe0PEBp MWXEUXNQ M{HxOmlEPTB;Mz6z5qCK|ryP NGOwV2ozOjZzNEK0Ny=>
CEM-S M1rBXGN6fG:2b4jpZ{BCe3OjeR?= MWGxNEDPxE1? MV20PEBp NHPBWXJFVVOR M{T2OWlEPTB;NT6x5qCK|ryP MYOyNlYyPDJ2Mx?=
MOLT-4 MVzGeY5kfGmxbjDBd5NigQ>? MVGxNEDPxE1? MVeyOEBp M3HTVWROW09? MWTCcI9kc3NiY3XscJMhcW5idHjlJGcxN0dzIIDoZZNmKG:oIITo[UBk\WyuIHP5Z4xm M1fqfFIzPjF2MkSz
MOLT-4 MkXXSpVv[3Srb36gRZN{[Xl? M2[0bVTjiIoQvF2= M1HYblQhcA>? M4TtZWROW09? MV3JcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFM1GvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> NGT0VG8zOjZzNEK0Ny=>
CEM-R MX\GeY5kfGmxbjDBd5NigQ>? M2q1T|TjiIoQvF2= M1nLTlQhcA>? MYXEUXNQ Ml73TY5kemWjc3XzJJRp\SCjbX;1cpQhd2ZiY3zlZZZm\CCOQ{TBM2ItKGFid3XscE1me3SjYnzpd4hm\CCjdYTvdIhi\3libXHyb4Vz MmfkNlI3OTR{NEO=
CEM-S M3zrSmZ2dmO2aX;uJGF{e2G7 NXz3VHBwPOLCid88US=> NE\uPY81KGh? NYHPSJRQTE2VTx?= NWDDWox3UW6lcnXhd4V{KHSqZTDhcY92dnRib3[gZ4xm[X[nZDDMR|VCN0JuIHGge4VtdC2nc4ThZoxqe2inZDDheZRweGijZ4mgcYFzc2W{ M1y1T|IzPjF2MkSz
HepG2 cell NUi3fItvU2mwYYPlJGF{e2G7 M{HHeVIxKM7:TR?= MXGyOEBp M2nwT2ROW09? M2mxO2Rwf26{ZXf1cIF1\XNidHjlJJBpd3OyaH;yfYxifGmxbjDv[kBCU1R? MnewNlM4QTd|MUm=
HepG2 cell M1uxeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEj3d4k{OCEQvF2= MlrVNlQhcA>? M3jGNWROW09? NF24b2xKdmirYnn0d{Bk\WyuIHfyc5d1cA>? NHXrT2czOzd7N{OxPS=>
HepG2 cell NGjQVYFCeG:ydH;zbZMhSXO|YYm= NHzsTpczOCEQvF2= NFHYTHczPCCq NV76U5ZvTE2VTx?= MUDJcoR2[2W|IHPlcIwh[XCxcITvd4l{ MlfhNlM4QTd|MUm=
GEO M1f0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoG0OVAxKG6P NVrLOWJ5PzJiaB?= NULXNmw4TE2VTx?= NGnld5RKdmirYnn0d{Bk\WyuIHfyc5d1cA>? M{n5NlI1PThzMkOx
CNE-1 NHjrOpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rNXVExKM7:TR?= Mn3oPVYhcA>? NEHjfoJFVVOR NXjFNJQ3UUN3ME2yMlk3KM7:TR?= NVjM[W1{OjV|M{[5NlU>
CNE-2 NXPuUGF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVKxNEDPxE1? M2L2blk3KGh? NIHMV4VFVVOR M2L1d2lEPTB;ND61N{DPxE1? MX:yOVM{Pjl{NR?=
HONE-1 M2[3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFy4fZgyOCEQvF2= MX:5OkBp NHjXNnRFVVOR NInFPWdKSzVyPUOuN|ch|ryP NYC0b29HOjV|M{[5NlU>
SUNE-1 NUnSU5JOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XWc|ExKM7:TR?= NHnxb3I6PiCq NXK0WZhuTE2VTx?= NHm5OmtKSzVyPUCuOVIh|ryP M{H3WFI2OzN4OUK1
CNE-2 MkLzSpVv[3Srb36gRZN{[Xl? MYqxNEDPxE1? M3jpRlQ5KGh? M{PRR2ROW09? NIqxdpBKdmS3Y3XzJINmdGxiY4njcIUh[XK{ZYP0JIF1KEdz NYPnVVlXOjV|M{[5NlU>
HONE-1 NU\0SG5ETnWwY4Tpc44hSXO|YYm= NUHJT49yOTBizszN MkS3OFghcA>? NHjIfpJFVVOR NWXlXJh3UW6mdXPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBifCCJMR?= M2HuTFI2OzN4OUK1
NEC8 NXX6WYdRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4[0XmlEPTB;MD6wPVY2OSEQvF2= MkHuV2FPT0WU
P12-ICHIKAWA MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\IcHZ4UUN3ME2wMlEyPjJizszN NYPIbXJEW0GQR1XS
MDA-MB-175-VII NY\BUWh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLtWWhlUUN3ME2wMlE{PzN6IN88US=> NW\l[m1DW0GQR1XS
AsPC-1 M4D2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwMkKxNlIh|ryP MlLBV2FPT0WU
T47D MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDsUmZKSzVyPUCuNlgzPSEQvF2= MmTJV2FPT0WU
HH NYrNPHd4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTBwM{CyPFMh|ryP NYjjVHJCW0GQR1XS
MOLT-16 M13VOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPSTWM2OD1yLkOwN|Ih|ryP NV3IO|F[W0GQR1XS
ES5 MkjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjmOmNIUUN3ME2wMlM1PDV3IN88US=> MUfTRW5ITVJ?
RS4-11 Mn7qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Moq4TWM2OD1yLkO0OlEh|ryP NHG5VHpUSU6JRWK=
KARPAS-45 MlLTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwM{ezNlEh|ryP NVy5TG5[W0GQR1XS
NCI-H720 M4HLTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7kc5NDUUN3ME2wMlM4Pjd7IN88US=> M2LoVHNCVkeHUh?=
H9 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwM{i4PFMh|ryP MY\TRW5ITVJ?
EFM-19 M3HOU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTBwNESwNUDPxE1? M1H6cnNCVkeHUh?=
SBC-1 M2nGV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTqb2ZuUUN3ME2wMlQ1ODN3IN88US=> M2iydXNCVkeHUh?=
A4-Fuk NEj2OXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlziTWM2OD1yLkS2PFY5KM7:TR?= MoTnV2FPT0WU
NCI-H1563 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLxfoN2UUN3ME2wMlQ5OTh7IN88US=> MYjTRW5ITVJ?
HCC1419 Ml[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHYbplKSzVyPUCuOFg5QTJizszN NUThVJJwW0GQR1XS
H-EMC-SS MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4S0T2lEPTB;MD60PVk{QSEQvF2= Mm\GV2FPT0WU
BHT-101 NUT0XpNYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXMNJpKSzVyPUCuOVI6PjFizszN NH7RTZJUSU6JRWK=
IGROV-1 NETkO4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HibGlEPTB;MD61OVI1QSEQvF2= Mor6V2FPT0WU
HGC-27 NXP6bHozT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrFRYxnUUN3ME2wMlU3Pzh|IN88US=> NFq1NXFUSU6JRWK=
MDA-MB-361 M4OxNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjhRXFKSzVyPUCuOVc4PjFizszN MV;TRW5ITVJ?
KE-37 NH\NXnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HNN2lEPTB;MD61PFI3KM7:TR?= MUHTRW5ITVJ?
HCC70 NUTHbWtrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFziWpBKSzVyPUCuOVk5OjdizszN Ml2zV2FPT0WU
LNCaP-Clone-FGC MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTBwNkGwOFgh|ryP MkfOV2FPT0WU
HAL-01 NV3DXphCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwNkKxN{DPxE1? NFHTUIlUSU6JRWK=
HT MnPYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFX5RWtKSzVyPUCuOlMzOzlizszN MVnTRW5ITVJ?
MDA-MB-415 NHH3UYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLCbohKSzVyPUCuOlM3OjZizszN M4Tje3NCVkeHUh?=
NOS-1 MkjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTVWYdWUUN3ME2wMlY{PzJ|IN88US=> NWPofHQ6W0GQR1XS
DU-145 NXrxOY9PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7iTWM2OD1yLk[0O|Q2KM7:TR?= NHzYeHNUSU6JRWK=
OCUB-M NFfFPYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELDN4RKSzVyPUCuO|A6PjZizszN MX7TRW5ITVJ?
VA-ES-BJ M1u1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTFTWM2OD1yLkezNFI2KM7:TR?= MYrTRW5ITVJ?
J-RT3-T3-5 M2rYU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfZOJdKSzVyPUCuO|Q1ODNizszN MlnTV2FPT0WU
MOLT-4 Ml;ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwOEC1PFIh|ryP MWHTRW5ITVJ?
NB7 Mn;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnWTWM2OD1yLkiyOFEyKM7:TR?= NIjNZoJUSU6JRWK=
L-363 NYnoRms4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTSbZZJUUN3ME2wMlg{PDR{IN88US=> NF3jW4pUSU6JRWK=
NKM-1 NF3FdYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvhRplKSzVyPUCuPFYzPTNizszN MYXTRW5ITVJ?
HOP-92 M4LDcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTBwOEeyNlMh|ryP MVHTRW5ITVJ?
OAW-42 NGfhc|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLWTWM2OD1yLki4O|Ih|ryP NWizdI1qW0GQR1XS
HuO9 NF;B[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwOUK3OVEh|ryP NYS0dYdKW0GQR1XS
MFE-280 NH3QTpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUn1XmN{UUN3ME2wMlk3PDZ3IN88US=> MmHOV2FPT0WU
EM-2 M33rZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jUR2lEPTB;MD65O|k{QSEQvF2= MXzTRW5ITVJ?
NCI-H520 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3VTWM2OD1yLkm4OVkzKM7:TR?= NEXYW3lUSU6JRWK=
LB2241-RCC M{PXbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIr0fpFKSzVyPUCuPVk4OzRizszN NF3FOIhUSU6JRWK=
SK-NEP-1 NX[3OnI5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTFwMUS0PFUh|ryP Mn;0V2FPT0WU
LXF-289 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XTbGlEPTB;MT6xO|E2PiEQvF2= MlryV2FPT0WU
EPLC-272H NEDqR5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHBTWM2OD1zLkG3NlU3KM7:TR?= NHT1WJVUSU6JRWK=
COLO-684 NGq1NVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTFwMkO3NlUh|ryP Mnn6V2FPT0WU
ES1 MlfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvOSpdxUUN3ME2xMlI1ODZ3IN88US=> Mmj6V2FPT0WU
DOHH-2 Ml[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnETWM2OD1zLkK4NlA{KM7:TR?= M1jFZ3NCVkeHUh?=
CTB-1 NXrnXYlrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmD1TWM2OD1zLkK4PVkh|ryP NVnqeo9CW0GQR1XS
G-401 M3;VSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHT6fWRKSzVyPUGuNlk4QTVizszN NYThXZVCW0GQR1XS
LoVo NYSyVIR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPXXYpJUUN3ME2xMlMzPTN2IN88US=> MoDmV2FPT0WU
Ramos-2G6-4C10 M36yfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofoTWM2OD1zLkOzO|AyKM7:TR?= MYTTRW5ITVJ?
MFM-223 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTFwM{S0OlEh|ryP NEXY[3pUSU6JRWK=
PA-1 NX3DTllDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTFwM{WyOlUh|ryP M1TWenNCVkeHUh?=
697 MnLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFy3XHdKSzVyPUGuN|c3OTZizszN M4LRWHNCVkeHUh?=
QIMR-WIL NX7UNWRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zxTWlEPTB;MT60PVEyPiEQvF2= M3rhNXNCVkeHUh?=
HOS NXLoOZdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTFwNEm1OVgh|ryP M3nkUnNCVkeHUh?=
DMS-273 MlHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DtVGlEPTB;MT61NVk2QSEQvF2= NV3wempwW0GQR1XS
ME-180 MmPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3Z[XlKSzVyPUGuOVY5QTFizszN M1PGN3NCVkeHUh?=
HCC2218 M13UcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITDeoVKSzVyPUGuOlgzOjVizszN M2j4SHNCVkeHUh?=
CAL-54 MojQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPlTWM2OD1zLkexNlQzKM7:TR?= MmrYV2FPT0WU
OMC-1 M{nhSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPRbGhmUUN3ME2xMlc1Pjd5IN88US=> MlThV2FPT0WU
COR-L105 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjZTWM2OD1zLke5O|M4KM7:TR?= NVTidGZnW0GQR1XS
BV-173 NXTieIhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHZOWRuUUN3ME2xMlgyODd2IN88US=> M1HQR3NCVkeHUh?=
RKO NXm4c|RYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHuxT|VKSzVyPUGuPFcyODFizszN MmC1V2FPT0WU
SNU-387 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDCTWM2OD1zLki4OFA3KM7:TR?= M1vDZ3NCVkeHUh?=
SW1088 NHv3WIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTFwOUS2NFYh|ryP MnTsV2FPT0WU
Hs-578-T MnnIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHBNYJKSzVyPUKuNVE1OzNizszN MWPTRW5ITVJ?
OC-314 MmHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUn3RlJ3UUN3ME2yMlE2ODh4IN88US=> NVXVN2xwW0GQR1XS
RMG-I NF[5SZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrYR2dKSzVyPUKuNVY{QThizszN MmLQV2FPT0WU
NCI-H1395 NVfpZ3k1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{m1VWlEPTB;Mj6xPFA6OSEQvF2= MlTGV2FPT0WU
GAMG NFP2bVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\GVWlEPTB;Mj6yN|g1PSEQvF2= MVPTRW5ITVJ?
LB1047-RCC NHzlfG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnxfWR4UUN3ME2yMlI1OzF5IN88US=> NX3ZTFdDW0GQR1XS
MN-60 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD0PWNQUUN3ME2yMlI6QTJ|IN88US=> M{\ZXXNCVkeHUh?=
OAW-28 MmixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzWd4UyUUN3ME2yMlI6QTVzIN88US=> Ml\oV2FPT0WU
NCI-H2228 NXj6bVhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPydJd1UUN3ME2yMlMyPTV{IN88US=> MXzTRW5ITVJ?
ABC-1 NE\C[25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoD0TWM2OD1{LkOzNlU{KM7:TR?= NEXCOHFUSU6JRWK=
LS-513 M{fxXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;RcoZKSzVyPUKuN|M1QDRizszN MlztV2FPT0WU
KS-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTJwM{ixPVEh|ryP NYPj[pFLW0GQR1XS
NB69 M1vzdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTJwM{i5PFMh|ryP MWnTRW5ITVJ?
VM-CUB-1 M3XrUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJwM{mwPFMh|ryP MmjnV2FPT0WU
D-423MG Ml:0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTJwNEGwOFQh|ryP NWPJUY5iW0GQR1XS
EW-18 M3W0W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTJwNEG5N|kh|ryP NELFSVVUSU6JRWK=
YH-13 NYDsT3RLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkO0TWM2OD1{LkS2NVU{KM7:TR?= NV\LbGdxW0GQR1XS
T-24 NIm4dHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrIN|hKSzVyPUKuOFc5QDFizszN MVrTRW5ITVJ?
ES8 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTJwNEmyPFch|ryP MXzTRW5ITVJ?
ES3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTJwNEm3OVkh|ryP MWjTRW5ITVJ?
RXF393 MoLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LYfGlEPTB;Mj62NFQ5PyEQvF2= MljHV2FPT0WU
RPMI-8226 NU\WV286T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTJwNkK5OVMh|ryP NHjMXZJUSU6JRWK=
AGS M{Pnemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DZdGlEPTB;Mj63NlE{PyEQvF2= NXjjU|RtW0GQR1XS
HCC1395 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moi0TWM2OD1{Lke1NVg4KM7:TR?= NWXUUoMyW0GQR1XS
MV-4-11 M13jb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfNTWM2OD1{Lke1NlY3KM7:TR?= M{ToZXNCVkeHUh?=
A204 M{D2NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXG2OZFwUUN3ME2yMlg{QDd{IN88US=> NF;zZVlUSU6JRWK=
MCF7 M1HXV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXn2SGJNUUN3ME2yMlg3OTF5IN88US=> NWH3T2M4W0GQR1XS
SNU-423 MnP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3qzU2lEPTB;Mj64PVI1OiEQvF2= MWjTRW5ITVJ?
NCI-H1048 M4nlSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjJXld5UUN3ME2yMlk3QDZ3IN88US=> MnnaV2FPT0WU
GR-ST M{HPXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTBTWM2OD1|LkC0OlEyKM7:TR?= MXrTRW5ITVJ?
EoL-1- NEPVNJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jEZ2lEPTB;Mz6wO|A2QCEQvF2= NWTBcVBbW0GQR1XS
HuH-7 NGHESmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\GemZFUUN3ME2zMlA6PDZ2IN88US=> MmjPV2FPT0WU
OS-RC-2 M1Hsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHKTWM2OD1|LkGxNVkh|ryP NWHpepl[W0GQR1XS
EW-3 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTNwMUm1Nlkh|ryP MWDTRW5ITVJ?
NCI-H747 NH3nT|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TqOmlEPTB;Mz6yNFY6PCEQvF2= MWTTRW5ITVJ?
EW-16 M{m0V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fvRWlEPTB;Mz6yNVg4QSEQvF2= M{DnZnNCVkeHUh?=
DOK MoXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF60O5BKSzVyPUOuNlI5PTlizszN NIn1WIFUSU6JRWK=
HCC2157 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF[yTXZKSzVyPUOuN|gyPzlizszN NVnrfG5kW0GQR1XS
OVCAR-3 M33YXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TwVGlEPTB;Mz60NFc5PiEQvF2= NH\zVHdUSU6JRWK=
NCI-H1623 NVP0cmN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTNwNEGyNlQh|ryP M2L2WHNCVkeHUh?=
H4 MlO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWr2dYxvUUN3ME2zMlQ2PjJ4IN88US=> MnnhV2FPT0WU
SW1710 M2rrTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\uOVlKSzVyPUOuOFY3PzhizszN MWDTRW5ITVJ?
RT-112 Mk\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{X1UWlEPTB;Mz61NlM5QCEQvF2= NGPBNZFUSU6JRWK=
DMS-114 NVmxT4JnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTNwNkKyO|gh|ryP M4\PTHNCVkeHUh?=
AN3-CA MmPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF21blZKSzVyPUOuOlI1PTZizszN NEn6OFZUSU6JRWK=
KNS-62 NVy3WI5bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3yTm02UUN3ME2zMlY{OzN6IN88US=> NVHZUYFJW0GQR1XS
SJRH30 NWfvVpBvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17UOGlEPTB;Mz62PVEzOiEQvF2= MmDUV2FPT0WU
G-402 NF3sZYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTNwN{C3NVEh|ryP NHjVcnlUSU6JRWK=
MHH-PREB-1 MnHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHewOoJKSzVyPUOuO|IxOzhizszN NInpSmxUSU6JRWK=
P30-OHK NEPRdIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DLbmlEPTB;Mz64NFk4PiEQvF2= M4LYeXNCVkeHUh?=
RVH-421 M1Kze2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XEPGlEPTB;Mz64NVc5QCEQvF2= Mlq3V2FPT0WU
LU-134-A NEPpcYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTNwOEi0Nlgh|ryP NYjvZnBGW0GQR1XS
ECC10 NUL2No9WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWiwZ5ZxUUN3ME2zMlk{PjJ{IN88US=> M1rUdnNCVkeHUh?=
TGW M{fhc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfISmhiUUN3ME20MlAzOzB3IN88US=> MVPTRW5ITVJ?
MLMA M3z0VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTRwMEK5OlYh|ryP MkXQV2FPT0WU
SCC-25 NIOzOJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3tTWM2OD12LkC2OVY3KM7:TR?= NIW3eJJUSU6JRWK=
TYK-nu M4HlVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j4VmlEPTB;ND6wPVU{PCEQvF2= MmrVV2FPT0WU
LAMA-84 M1rSUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITaZVRKSzVyPUSuNVQyQTFizszN MVnTRW5ITVJ?
Calu-3 NV3VNHdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfnU2hRUUN3ME20MlI1PDF4IN88US=> NGTLT4JUSU6JRWK=
NCI-H460 NV3wWoFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHZN3JKSzVyPUSuNlY1PDNizszN MWjTRW5ITVJ?
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NCI-H292 NUW2[INZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTRwM{ixOFYh|ryP M33vO3NCVkeHUh?=
HCE-T M3:3RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTPeZliUUN3ME20MlQyPTd7IN88US=> M2nMNnNCVkeHUh?=
EW-11 M4LqeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTRwNEG4N|gh|ryP MVXTRW5ITVJ?
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KLE NY[0dVZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvS[HhKSzVyPUSuO|AyQThizszN NE\aeVdUSU6JRWK=
CAL-39 M{DKOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYG2[YlCUUN3ME20MlczOTR4IN88US=> NXzYXVJzW0GQR1XS
TI-73 NYnOUYloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTHTWM2OD12LkiwOlA6KM7:TR?= MUfTRW5ITVJ?
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NCI-H446 NGTQVoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjaTnZXUUN3ME21MlIxODB7IN88US=> NHrBbHpUSU6JRWK=
ETK-1 NFy0T5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPtdpRZUUN3ME21MlIyOTZ3IN88US=> NXnnUm1[W0GQR1XS
BT-20 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTVwMkGzOVMh|ryP NY\PZ|ZiW0GQR1XS
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CAL-27 NETZSItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTVwNE[zN|kh|ryP M2[xXHNCVkeHUh?=
SW872 MnnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorrTWM2OD13LkW5OFI5KM7:TR?= MUDTRW5ITVJ?
RPMI-2650 Ml7qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorWTWM2OD13Lk[2NVk6KM7:TR?= NVPWOnVzW0GQR1XS
PFSK-1 NFnYcZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrqTWM2OD13LkeyO|MzKM7:TR?= NETINnhUSU6JRWK=
SF295 NXjVZnh7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;obFZNUUN3ME21MlgxPjN|IN88US=> NXzQUnFSW0GQR1XS
Becker M1T0cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIm0fZlKSzVyPUWuPFY1PzJizszN NFPBe|BUSU6JRWK=
Saos-2 MmXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEe5WmxKSzVyPUWuPFY2OyEQvF2= NVPPcIJKW0GQR1XS
SK-OV-3 M3XEPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnre4poUUN3ME21Mlk6QDF4IN88US=> NIXjcoNUSU6JRWK=
VMRC-RCZ NWT1WIUyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn:yTWM2OD14LkC4O|c{KM7:TR?= M3zQUXNCVkeHUh?=
EW-22 NVTRUYdDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTZwMUm2OFkh|ryP MoThV2FPT0WU
BT-474 NVXLSHo2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfsSZNKSzVyPU[uNlM{KM7:TR?= MnH5V2FPT0WU
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NB12 M4Wxdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4D6WmlEPTB;Nj6zPVA4OSEQvF2= M1jRbXNCVkeHUh?=
D-263MG M{XWS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3e2SmlEPTB;Nj60OVE3QSEQvF2= M1fzR3NCVkeHUh?=
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A704 MmjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTZwNkOwOkDPxE1? M1Kw[3NCVkeHUh?=
NCI-H1693 NI\wUFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXvTWM2OD14Lk[zOlA1KM7:TR?= NFu0dYlUSU6JRWK=
LN-405 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTZwN{m2O|Ih|ryP MX\TRW5ITVJ?
CHL-1 NEjVVIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jSS2lEPTB;Nj64NFA4QSEQvF2= MkjJV2FPT0WU
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TE-6 NV3MeYtqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUX5WmlUUUN3ME22Mlk{ODN6IN88US=> MVrTRW5ITVJ?
AU565 NV7USI5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrXZZhxUUN3ME22Mlk3QTV5IN88US=> MVjTRW5ITVJ?
RD NILsbZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX6yPXB1UUN3ME22Mlk5Ojh2IN88US=> MljuV2FPT0WU
SW1463 NEDtT3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTdwMUGxOlgh|ryP NYWycpVNW0GQR1XS
LU-99A MmXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPWXGs2UUN3ME23MlE1OzJ{IN88US=> Mm\BV2FPT0WU
NCI-H28 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mni0TWM2OD15LkK5NlQh|ryP MoXYV2FPT0WU
MC-IXC M{nLZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTdwNEi1O|Yh|ryP MVXTRW5ITVJ?
GP5d MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Lsd2lEPTB;Nz60PFc3PCEQvF2= NGSxeohUSU6JRWK=
GB-1 M3rXfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVr5b5R5UUN3ME23MlU1QDB2IN88US=> MnzMV2FPT0WU
CAL-33 NWLSV4trT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETBPFBKSzVyPUeuOlYzOzNizszN MVrTRW5ITVJ?
MSTO-211H NIDNR|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2K2T2lEPTB;Nz62O|M{PiEQvF2= M36yfXNCVkeHUh?=
TE-5 NXHqU3RDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTdwN{mzN|Qh|ryP MknkV2FPT0WU
D-566MG M2exb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DyTGlEPTB;OD6wOFQzQSEQvF2= M3jxfHNCVkeHUh?=
JVM-3 Ml62S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3nOIdCUUN3ME24MlE2OjZ6IN88US=> MlPLV2FPT0WU
T98G NWqxO21YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLtTWM2OD16LkG4NFY4KM7:TR?= MYnTRW5ITVJ?
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LB996-RCC MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LSRWlEPTB;OD61N|I2PyEQvF2= MVjTRW5ITVJ?
SKG-IIIa MmTKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\STWM2OD16Lk[zNFY6KM7:TR?= MVjTRW5ITVJ?
NCI-SNU-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHsTWM2OD16Lk[0OlQ{KM7:TR?= NGjicIxUSU6JRWK=
LB771-HNC M{Xpfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{GzWmlEPTB;OD62OFY6PiEQvF2= MVLTRW5ITVJ?
SCC-4 M{SyNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnSxTWM2OD16Lk[4NlE6KM7:TR?= Mmf6V2FPT0WU
CAMA-1 NHjuN21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRThwN{exOFYh|ryP NYrNTGxjW0GQR1XS
D-502MG M3;GVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUOwbGJYUUN3ME24Mlc5PjJ7IN88US=> NIr3W|BUSU6JRWK=
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HEC-1 NVHROmNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVizUVhrUUN3ME24Mlg6QDZ4IN88US=> MVzTRW5ITVJ?
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HN M3HmSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojWTWM2OD17LkG2OlI5KM7:TR?= MnjsV2FPT0WU
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SHP-77 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1T2eWlEPTB;OT6zPVk{PSEQvF2= MVzTRW5ITVJ?
ACN MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnnTWM2OD17LkWzNlc4KM7:TR?= NGLnU4lUSU6JRWK=
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MDA-MB-157 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLRcmwyUUN3ME25MlkzQDd6IN88US=> M1X5WHNCVkeHUh?=
A101D NI\hZY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{O1dmlEPTB;OT65PVk4PCEQvF2= NV32[FNZW0GQR1XS
YKG-1 M{DSPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTFyLkKwNFYh|ryP M16xcHNCVkeHUh?=
LAN-6 M3jZRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rDT2lEPTB;MUCuNlE3PCEQvF2= M3jDZnNCVkeHUh?=
OVCAR-5 NVjOVYtWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjo[mNKSzVyPUGwMlI1OzNizszN MX;TRW5ITVJ?
A549 NFTFb|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPhXHk1UUN3ME2xNE4{QTd|IN88US=> NU\wd45UW0GQR1XS
no-11 NV7OPZVbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LE[2lEPTB;MUCuOFM2OyEQvF2= NITveoRUSU6JRWK=
SF539 NUO5dnB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrnR5l6UUN3ME2xNE46ODRzIN88US=> NEHkNnlUSU6JRWK=
A388 Ml7hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWW4Z5pyUUN3ME2xNU4{QDl5IN88US=> MWHTRW5ITVJ?
DEL MkPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jmfmlEPTB;MUGuOFI1KM7:TR?= Mn3rV2FPT0WU
SW954 MlTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DaZmlEPTB;MUGuOFY3QCEQvF2= NGTXPXJUSU6JRWK=
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SW756 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnifotkUUN3ME2xNU42Ojl2IN88US=> NYjORoZQW0GQR1XS
PC-3 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTFzLkW3OlQh|ryP MX3TRW5ITVJ?
ONS-76 Ml7yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfPPHlKSzVyPUGxMlY{PiEQvF2= MYXTRW5ITVJ?
A427 NFjS[2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPVZ|NKSzVyPUGxMlcxQTNizszN M4H1dnNCVkeHUh?=
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NCI-H1299 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzmNFdKSzVyPUGxMlgxQTNizszN NWC4S2NFW0GQR1XS
GCT M4rSUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTFzLkiyNlgh|ryP M2XPSHNCVkeHUh?=
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CAL-51 NGHJepFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXX2SlIyUUN3ME2xNk4xPzF4IN88US=> NXPs[lBtW0GQR1XS
BB49-HNC NFjlcXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrjRWdnUUN3ME2xNk4yOTd5IN88US=> NUDlcIoxW0GQR1XS
RPMI-7951 NIPYW3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrOUI9KSzVyPUGyMlE5PTRizszN MXLTRW5ITVJ?
RH-1 M1fYUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTF{LkKxPFQh|ryP MnvwV2FPT0WU
BCPAP NIPNR5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PhcmlEPTB;MUKuOFc1QSEQvF2= M1PCe3NCVkeHUh?=
GCIY MnH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTF{LkWyNFkh|ryP NGX2PGNUSU6JRWK=
KNS-81-FD MmDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrpXHNzUUN3ME2xNk42QDZ7IN88US=> MmPNV2FPT0WU
KYSE-140 Mn7NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v6VGlEPTB;MUKuPFU6PSEQvF2= M4HZ[XNCVkeHUh?=
Ca-Ski Moe2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXe4Z5VIUUN3ME2xNk46ODRzIN88US=> NHe1[3dUSU6JRWK=
TGBC1TKB MnnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjES5F3UUN3ME2xNk46OTF3IN88US=> NVPOVohFW0GQR1XS
HCC1187 M3Wzd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFr1U5JKSzVyPUGzMlE6OTJizszN MnTyV2FPT0WU
SJSA-1 NVLqUXVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU[0ZnRbUUN3ME2xN{4zOzJ5IN88US=> NF30dYlUSU6JRWK=
CTV-1 NFHUfW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXETWM2OD1zMz6zOFUh|ryP MYfTRW5ITVJ?
WM-115 M4DSUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHH3boVKSzVyPUGzMlY1QDNizszN MlXiV2FPT0WU
CHP-212 M2Xjbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3rRW5UUUN3ME2xN{46PzN7IN88US=> M1HpdHNCVkeHUh?=
SCC-15 NVXUSXd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTuNndqUUN3ME2xN{46Pzd3IN88US=> NF;xUJBUSU6JRWK=
BPH-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPlN5duUUN3ME2xOE4yPjZ2IN88US=> NXTlPWZTW0GQR1XS
SW780 NYHlXVVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF60OndKSzVyPUG0MlUxOjVizszN M3PaUnNCVkeHUh?=
NCI-H2291 NXLyXXp4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTF2LkW4O|gh|ryP NVHkN49vW0GQR1XS
JEG-3 NF\pSHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37teWlEPTB;MUSuOlMzPiEQvF2= MmXmV2FPT0WU
CAL-120 MkP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTF2LkewNlch|ryP MlvRV2FPT0WU
NCI-H23 NWmwUlFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\iOZZHUUN3ME2xOE44QTl5IN88US=> MmLYV2FPT0WU
MS-1 M1HRXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPmTWM2OD1zND65OlEyKM7:TR?= MYLTRW5ITVJ?
PC-14 NEDnU|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;HXFhMUUN3ME2xOE46PjV2IN88US=> Ml7xV2FPT0WU
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CCRF-CEM Mni1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITqOGdKSzVyPUK0N{44QTVizszN NIWyT5RUSU6JRWK=
SH-4 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3r1fGlEPTB;MkS2MlA6KM7:TR?= MlTHV2FPT0WU
LS-1034 NYPmemxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DHZmlEPTB;MkS2MlI3PiEQvF2= NYf4OnFlW0GQR1XS
NCI-H2347 M17Bfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3pOFFKSzVyPUK0O{44OTNizszN M2HhNXNCVkeHUh?=
RPMI-8866 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTJ2OT6yO{DPxE1? M1yxcXNCVkeHUh?=
GAK MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTJ3Mz6wNFIh|ryP NWLoR4ttW0GQR1XS
NB6 NVnUcFNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTJ5MD6xJO69VQ>? M2j6U3NCVkeHUh?=
COLO-680N NGHaOItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofsTWM2OD1{N{KuOVI4KM7:TR?= M4f3VnNCVkeHUh?=
RERF-LC-MS MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHmxUIJKSzVyPUK3Ok4xODdizszN MUPTRW5ITVJ?
TGBC11TKB Mk\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnENJVKSzVyPUK3PE4yPzhizszN M3LidnNCVkeHUh?=
C8166 M2HLb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mom1TWM2OD1{N{iuOVA3KM7:TR?= MmnJV2FPT0WU
HDLM-2 NHPMfZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTJ7ND60NFkh|ryP MWnTRW5ITVJ?
IGR-1 NHXJe5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfUTWM2OD1{OUWuOlU6KM7:TR?= NW[wWpdOW0GQR1XS
FADU M2q4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TvO2lEPTB;Mkm3MlUyKM7:TR?= Mm\OV2FPT0WU
L-428 NV3Kbo5oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJ7Nz62NVYh|ryP NVPOfFhEW0GQR1XS
LU-65 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzMR3ZKSzVyPUOwOE4{OiEQvF2= NUjJdW1CW0GQR1XS
HEL NVGxdW5TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\5WmZKSzVyPUOwPU46QDNizszN NUDBdpJSW0GQR1XS
NCI-H810 M2fmSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULWZ4QxUUN3ME2zNVAvPTdizszN MnfzV2FPT0WU
C3A MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\OR4g{UUN3ME2zNVEvQDB{IN88US=> NUDxdHhZW0GQR1XS
NCI-H630 NYq4d|FYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTN|Mj6yPVQh|ryP MUjTRW5ITVJ?
KP-N-YN MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrCSGlKSzVyPUO0NU4yOjNizszN MVTTRW5ITVJ?
MOLT-13 MlL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHyTWM2OD1|NEKuN|I3KM7:TR?= NIHLWmpUSU6JRWK=
NCI-H1993 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXDTWM2OD1|NEKuN|Y2KM7:TR?= NF;SPGRUSU6JRWK=
BE-13 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY[0XFdyUUN3ME2zOFQvOTZ5IN88US=> NVXIZoNKW0GQR1XS
IST-SL1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTN2Nz60NFEh|ryP NIX5RW1USU6JRWK=
TE-9 NYr4fYVqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvTemRyUUN3ME2zOlMvPTh7IN88US=> MVTTRW5ITVJ?
LU-135 M1j0WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLYXpFYUUN3ME2zOlcvODN3IN88US=> MnG5V2FPT0WU
T84 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mom1TWM2OD1|N{SuO|EzKM7:TR?= Mlu3V2FPT0WU
K-562 Mnf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXsTWM2OD1|OEOuN|Yh|ryP NFXJZWJUSU6JRWK=
SBC-5 MmWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTN6Nj65PFUh|ryP M{HpbHNCVkeHUh?=
NB17 NIjXPIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTN7Mj61PVYh|ryP MoXjV2FPT0WU
NCI-H2052 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnIdXFqUUN3ME2zPVgvPDd{IN88US=> MnHBV2FPT0WU
HCC38 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUK5d4xUUUN3ME20NFEvPTl|IN88US=> MXrTRW5ITVJ?
NCI-H69 NH63bJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTR2MT6wPFMh|ryP NYnZcYg5W0GQR1XS

... Click to View More Cell Line Experimental Data

体内研究 MK-2206和这些细胞毒素药剂联用作用于NCI-H292移植瘤,MK-2206显示出非常有效的抗癌活性。卵巢癌A2780移植瘤中,按动物体重,每千克处理240mg MK-2206,则可抑制70%以上的Akt1/2在苏氨酸308位点和丝氨酸473位点的磷酸化,导致肿瘤生长抑制率达到60%。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[3]
+ 展开

Akt激酶实验:

通过得到的GSK 生物素肽段基质测定Akt激酶。使用含镧系金属螯合物偶联单抗的均相时间分辨荧光(HTRF)测定肽段磷酸化程度,这种单抗专门作用于与链霉亲和素——藻蓝素(SA-APC)载体相结合的磷酸化作用,SA-APC载体可以结合到肽段的部分生物素上。当镧系金属螯合物与藻蓝素相接触时,无辐射的能量从镧系金属螯合物传递给藻蓝素,然后藻蓝素发射波值为655纳米的光线。工作液:100X 蛋白酶抑制剂混合物(PIC):1mg/ml苯甲脒,0.5 mg/ml 抑肽素, 0.5 mg/ml亮抑肽酶, 0.5 mg/ml 抑肽酶。10X实验 buffer: 500 mM HEPES(pH 为7.5), 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-甘油磷酸。抑制 buffer:50 mM HEPES(pH为7.3), 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM 标记的单抗, 0.0067mg/ml SA-APC。ATP/MgCl2工作液: 1X 实验buffer, 1 mM DTT, 1X PIC, 5% 甘油, 激活的Akt。肽段工作液:1X实验buffer, 1 mM DTT, 1X PIC, 5% 甘油 2 TM GSK生物素肽段。工作液加到合适的孔中,然后加入总负荷为16的 ATP/MgCl2,反应开始。含MK-2206的实验组和对照组加入总负荷为10的肽段工作液。然后加入总负荷为13的酶液,混匀。反应进行50分钟,然后加入总负荷为60的均相时间分辨荧光抑制buffer停止反应。阻断的反应在室温下温育30分钟以上,然后使用仪器读数。
细胞实验:[2]
+ 展开
  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6,和NCI-H460细胞
  • Concentrations: 0, 0.3, 1,和3 μM
  • Incubation Time: 72或96小时
  • Method: MK-2206溶解在DMSO中,在实验使用前用培养基稀释。细胞按2~3×103密度接种在96孔板上,温育24小时。MK-2206 (分为0, 0.3, 1,和3 μmol四组)处理细胞,72或者96小时后,测定细胞增殖情况。
    (Only for Reference)
动物实验:[2]
+ 展开
  • Animal Models: 携带SK-OV-3, NCI-H292, HCC70, PC-3,和NCI-H460模型的雄性CD1裸鼠
  • Formulation: 30% Captisol
  • Dosages: 120 mg/kg
  • Administration: 口服处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 14 mg/mL (29.14 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次加入纯溶剂:
15% Captisol
17 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 480.39
化学式

C25H21N5O.2HCl

CAS号 1032350-13-2
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01306045 Recruiting Carcinoma, Non-Small-Cell Lung|Carcinoma, Small Cell Lung|Carcinoma, Thymic National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 21, 2011 Phase 2
NCT01802320 Active, not recruiting Colon Mucinous Adenocarcinoma|Colon Signet Ring Cell Adenocarcinoma|Rectal Mucinous Adenocarcinoma|Rectal Signet Ring Cell Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IIIA Colon Cancer|Stage IIIA Rectal Cancer|Stage IIIB Colon Cancer|Stage IIIB Rectal Cancer|Stage IIIC Colon Cancer|Stage IIIC Rectal Cancer|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer National Cancer Institute (NCI) March 2013 Phase 2
NCT01783171 Active, not recruiting Pancreatic Adenocarcinoma|Recurrent Pancreatic Carcinoma|Stage III Pancreatic Cancer|Stage IV Pancreatic Cancer|Unresectable Pancreatic Carcinoma National Cancer Institute (NCI) January 2013 Phase 1
NCT01776008 Active, not recruiting Estrogen Receptor Positive|HER2/Neu Negative|Recurrent Breast Carcinoma|Stage IIA Breast Cancer|Stage IIB Breast Cancer|Stage IIIA Breast Cancer|Stage IIIB Breast Cancer|Stage IIIC Breast Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01859182 Withdrawn Adenocarcinoma of the Gallbladder|Adenocarcinoma With Squamous Metaplasia of the Gallbladder|Adult Primary Cholangiocellular Carcinoma|Advanced Adult Primary Liver Cancer|Cholangiocarcinoma of the Extrahepatic Bile Duct|Localized Unresectable Adult Primary Liver Cancer|Metastatic Extrahepatic Bile Duct Cancer|Recurrent Adult Primary Liver Cancer|Recurrent Extrahepatic Bile Duct Cancer|Stage II Gallbladder Cancer|Stage IIIA Gallbladder Cancer|Stage IIIB Gallbladder Cancer|Stage IVA Gallbladder Cancer|Stage IVB Gallbladder Cancer|Unresectable Extrahepatic Bile Duct Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01705340 Terminated Adenocarcinoma of the Gastroesophageal Junction|HER2-positive Breast Cancer|Male Breast Cancer|Recurrent Breast Cancer|Recurrent Esophageal Cancer|Recurrent Gastric Cancer|Stage IIIC Breast Cancer|Stage IIIC Esophageal Cancer|Stage IIIC Gastric Cancer|Stage IV Breast Cancer|Stage IV Esophageal Cancer|Stage IV Gastric Cancer National Cancer Institute (NCI) September 2012 Phase 1

技术支持

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操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    What is your recommendation for preparing the solution and how would you deliver it to the mice (i.p. or gavage)?

  • 回答:

    You can resuspend MK-2206 in 15% Captisol at up to 17mg/ml. It's a suspension and is for oral gavage use only.

  • 问题 2:

    I would want to know if MK-2206 could cross the blood brain barrier?

  • 回答:

    MK-2206 can cross BBB based on the following reference: https://clinicaltrials.gov/ct2/show/NCT01249105.

Related Antibodies

Akt Signaling Pathway Map

Akt Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID