Luteolin

For research use only. Not for use in humans.

目录号:S2320 别名: Luteoline, Luteolol, Digitoflavone 中文名称:木犀草素,四羟黄酮

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin (Luteoline, Luteolol, Digitoflavone)是一种非选择性的PDE抑制剂,作用于PDE1-5Ki分别为15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM和9.5 μM。Phase 2.

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 582.73 现货
RMB 630.63 现货
RMB 1417.78 现货
RMB 4686.02 现货
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客户使用Selleck生产的Luteolin发表文献14篇:

客户使用该产品的1个实验数据:

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

产品安全说明书

ADC Cytotoxin抑制剂选择性比较

生物活性

产品描述 Luteolin (Luteoline, Luteolol, Digitoflavone)是一种非选择性的PDE抑制剂,作用于PDE1-5Ki分别为15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM和9.5 μM。Phase 2.
靶点
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
体外研究

Luteolin是在诃子中发现的黄酮类化合物,是非选择性的磷酸二酯酶PDE抑制剂,对PDE1-5的Ki值分别为15.0 μM,6.4 μM,13.9 μM,11.1 μM以及9.5 μM。[1] Luteolin抑制LPS刺激的TNF-α的产生,IC50值低于1 μM。Luteolin抑制LPS诱导的Akt和IkappaBalpha的磷酸化。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NXXnRXN4TnWwY4Tpc44h[XO|YYm= NVroZ5F3OiCq MoTzTY5pcWKrdHnvckBw\iCQTzDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW|I3PC55IHPlcIx{KHC{ZT3pcoN2[mG2ZXSg[o9zKDJiaILzJIJm\m:{ZTDMVHMhe3SrbYXsZZRqd25iZn;yJFI1KGi{czDifUBIemmnc4OgZZN{[XlibXX0bI9lNCCLQ{WwQVAvOjFizszN MVyyOVE4PjF6Nx?=
HEK293 FS cells M3rjSGZ2dmO2aX;uJIF{e2G7 NH7IZnFKdmirYnn0bY9vKG:oIF7PXFQh\XiycnXzd4VlKGmwIFjFT|I6OyCIUzDj[YxteyCjc4Pld5Nm\CCjczDINm8zKHC{b3T1Z5Rqd25iYomgTFJQOi:WeYKvUHBQKGG|c3H5MEBKSzVyPUCuPFUh|ryP MYKyNFc{OTN3Nx?=
CHO cells NIrjS4pHfW6ldHnvckBie3OjeR?= MVXB[49vcXO2IHHjeIl3cXS7IHH0JJJifCCGQWSg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiRVO1NF0yNjR3IN88US=> MXmyNFk4OTZ3MB?=
human MV4-11 cells NGP6SHdEgXSxdH;4bYNqfHliYYPzZZk> NXnKUoMzPzJiaB?= M{n1c2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1XPC1zMTDj[YxteyCqYYLic5JqdmdiRlzUN{BufXSjdHnvckBi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9NU44PiEQvF2= MmDlNlM1OTFyN{O=
HEK293 cells NH2x[2FHfW6ldHnvckBie3OjeR?= NHLw[ZQzPCCq NHnrXlJC\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5Nqdmdid3n0bEBI[Wx2IILldI9zfGW{II\lZ5RweiCjZoTldkAzPCCqcoOgZpkh\HWjbD3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR|UxRTJwMzFOwG0> MWqyOFk2PTh6OR?=
human U2OS cells MlexSpVv[3Srb36gZZN{[Xl? NXXK[FIzPSCq MlLMRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCJYXy0MXZROTZvVFXWJIF{e2W|c3XkJIF{KGKndHGgZZJz\XO2aX6geJJidnOub3PheIlwdiCjZoTldkA2KGi{czDifUBj\XSjIHzhZ5RidWG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUOuNkDPxE1? NF60SoMzPDlyMES0Oy=>
RBL-2H3 cells MYjGeY5kfGmxbjDhd5NigQ>? NIfqOJhKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUUxvNDDwdo9lfWO2aX;uJIlvKFKETD2yTFMh[2WubIOge4F{KGSndHXycYlv\WRuIFnDOVA:Oy55IN88US=> NVW4UldnOTJ7NUGwPVI>
human mast cells MWjGeY5kfGmxbjDhd5NigQ>? MmXGTY5pcWKrdHnvckBw\iCVWVugbY4hcHWvYX6gcYF{fCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gcYF{fCClZXzsJIRm\3KjboXsZZRqd25uIFXDOVA:PC53IN88US=> NYrzZ5BJOjJ{NUeyNVM>
human LNCAP cells NWTVNJRwTnWwY4Tpc44h[XO|YYm= NFfZSnRFd3ewcnXneYxifGmxbjDv[kBxem:|dHH0[UB{eGWlaX\pZ{BidnSrZ3XuJJNm[3KndHnvckBqdiCqdX3hckBNVkODUDDj[YxteyxiSVO1NF02KM7:TR?= NWCyNI5mOjVyMUm0O|g>
rat H9c2 cells M{f2OmZ2dmO2aX;uJIF{e2G7 MmW3NlQhcA>? NHTybpVEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGmwIILheEBJQWN{IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEWFNUC9OU42OyEQvF2= MnjDNlA6OzJ5NkK=
human HT-29 cells Mon6SpVv[3Srb36gZZN{[Xl? NU\odHN3OTBibXnudy=> NXnOb5MySWexbnnzeEBi[3Srdnn0fUBifCCJUGKzOUBz\WOncITvdkBqdiCqdX3hckBJXC1{OTDj[YxteyCjZoTldkAyOCCvaX7zJIJ6KGS7bnHtbYMhdWG|czDy[YRqe3S{aXL1eIlwdiCjc4PhfUwhTUN3ME23MlI1KM7:TR?= NUXTNYpqOjR7MEC0OFc>
human RS4:11 cells M3nKU2N6fG:2b4jpZ4l1gSCjc4PhfS=> NXzEW2hWPzJiaB?= MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSV|Q7OTFiY3XscJMhcGG{Yn;ybY5oKHerbHSgeJlx\SCITGSzJIFnfGW{IEeyJIhzeyCkeTD0[ZRz[XqxbHn1cUBj[XOnZDDFfkBEgVSxeDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhT0l3ME23MlI2KM7:TR?= MWOyN|QyOTB5Mx?=
NCI-H460 cells M17xNWZ2dmO2aX;uJIF{e2G7 NVz6Om9uOi1{MDDo NFnSU4FKdmirYnn0bY9vKG:oIFHCR2czKGW6cILld5Nm\CCrbjDoeY1idiCQQ1mtTFQ3OCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHBpSSCjY3P1cZVt[XSrb36gZYZ1\XJiMjD0c{AzOCCqcoOgdoVt[XSrdnWgeI8h\nWvaYTy[Y1wemerbjDDMEBKSzVyPUiuPUDPxE1? M2XUZlIyOjd3M{i2
human H9 cells NYi4enlUTnWwY4Tpc44h[XO|YYm= M17xTVMh\GG7cx?= NHTqNYhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:OTBizszN M13wclgyPThzNkS=
MDCK cells MoLvR5l1d3SxeHnjbZR6KGG|c3H5 MoKwR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSFSzDj[YxteyCkeTDNWHQh[XO|YYmsJGNEPTB;MUKuOFQh|ryP NHnaNFgyQDZ2MEC0Ni=>
mouse B16-4A5 cells M4n6dWZ2dmO2aX;uJIF{e2G7 NEHMXJc4OiCq NXvOW2hCUW6qaXLpeIlwdiCxZjD0bIVweGi7bHzpcoUue3SrbYXsZZRm\CCvZXzhco9o\W6nc3nzJIlvKG2xdYPlJGIyPi12QUWgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUG0JO69VQ>? MmHxNlAyQDl|OUm=
K562 cells MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGXtbIE2KGSjeYO= NV7BdFhDT3Kxd4ToJIlvcGmkaYTpc44hd2ZiS{W2NkBk\WyuczDifUBZXFRiYYPzZZkh[W[2ZYKgOUBl[Xm|LDDJR|UxRTF2Lk[1JO69VQ>? NIj3[IUyPzRzMUC5Ni=>
human H9 cells MmWxR5l1d3SxeHnjbZR6KGG|c3H5 NWn6O3N3OyCmYYnz M2Xo[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGg6KGOnbHzzJIFnfGW{IEOg[IF6eyxiSVO1NF0yPiEQvF2= NH;JZ4o5OTV6MU[0
human HT-29 cells M3TWZWZ2dmO2aX;uJIF{e2G7 M3TKb|ExKG2rboO= MYLE[ZNmdnOrdHn6ZZRqd25ib3[gS3BTOzVicnXj[ZB1d3JiaX6gbJVu[W5iSGStNlkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD6ZZBzcW6jc4StbY5lfWOnZDDkfY5idWmlIH3hd5MhemWmaYP0doljfXSrb36gZYZ1\XJiMUCgcYlveyxiSVO1NF0yQC54IN88US=> NYHBOZQ4OjR7MEC0OFc>
human U937 cells MUPQdo9tcW[ncnH0bY9vKGG|c3H5 NI[5UWQ4OiCq MmnKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXOUO3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCZU2StPEBie3OjeTygTWM2OD1{MDFOwG0> NXLUUnhOOTdzNUiwOVQ>
mouse HT22 cells NYDQe4VSTnWwY4Tpc44h[XO|YYm= M3f0NVMhcA>? NEXubmNP\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgbY4hdW:3c3WgTHQzOiClZXzsd{Bie3Onc4Pl[EBieyC2LVLPU2gucW6mdXPl[EB1d3irY3n0fUBifCB2MDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK|IH\vcIxwf2WmIHL5JJQuSk:RSDDpcoR2[3Srb36gcYVie3W{ZXSgZYZ1\XJiOTDodpMh[nliTWTUJIF{e2G7 MoHRNlQzPDV7M{m=
human THP1 cells M{PKbWZ2dmO2aX;uJIF{e2G7 MlrvNlAh|ryP MU[xJIg> MXfEc5dvemWpdXzheIlwdiCxZjDUVGEucW6mdXPl[EBPV1h{IH3SUmEh\XiycnXzd4lwdiCrbjDoeY1idiCWSGCxJINmdGy|IHH0JFIxKHWPIHnuZ5Vj[XSnZDDmc5IhOSCqcjDwdolweiC2bzDUVGEh[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFI1KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= NWPpSoZCOjN5OE[1NlA>
MDA-MB-231 cells MnvkSpVv[3Srb36gZZN{[Xl? MYe1JO69VQ>? MXixOkBp Mn:xTY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MorzNlUyQTB2Nk[=
HL60 cells MnvmVJJwdGmoZYLheIlwdiCjc4PhfS=> NU\z[YlkOzBizszN MlzIOFghcA>? MkjkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHH0JFMxKHWPIHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[Xl? MXSxPFI2QDR2MB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
体内研究 LD50:小鼠>2500毫克/千克(i.g.) [3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 286.24
化学式

C15H10O6

CAS号 491-70-3
储存条件 粉状
溶于溶剂
别名 Luteoline, Luteolol, Digitoflavone

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • 回答:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

免费分装抑制剂
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID