Staurosporine

目录号:S1421 别名: CGP 41251

Staurosporine Chemical Structure

Molecular Weight(MW): 466.53

Staurosporine是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。

规格 价格 库存 购买数量  
RMB 2186.89 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用该产品的3个实验数据:

  • Intracellular concentration of HSF1-phosphoserine 326, total HSF1, S6 kinase-phosphothreonine-389, total S6 kinase and β-actin, without or with heat shock in HeLa cells pretreated with mTOR inhibitors rapamycin (30 nM) and KU0063794 (2 uM) and kinase inhibitor staurosporine (100 nM) for 2 hr. Relative levels of HSF1-phosphoserine 326 in cells after the various treatments were determined by densitometric analysis of X-ray films, normalized to untreated cells (lane 1), and are indicated below the representation of the immunoblots.

    PLoS One 2012 7(6), e39679. Staurosporine purchased from Selleck.

    J Biomol Screen 2013 18(4), 388-99. Staurosporine purchased from Selleck.

  • J Biomol Screen 2013 18(4), 388-99. Staurosporine purchased from Selleck.

产品安全说明书

PKC抑制剂选择性比较

生物活性

产品描述 Staurosporine是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。
靶点
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外研究

Staurosporine 也强抑制HeLa S3细胞,IC50为4 nM。[1]Staurosporine 也抑制多种其他蛋白激酶,包括PKA, PKG, 磷酸激酶, S6 激酶, 肌球蛋白轻链激酶(MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, 和Syk , IC50分别为 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, 和 16 nM。[3] Staurosporine (1 μM) 诱导 PC12细胞90%以上凋亡,这种作用可被过量表达的Bcl-2抑制,或者zVAD-fmk, cycloheximide (10 μM) 及actinomycin D (5 μM)处理也可抑制。相应地, Staurosporine处理,诱导细胞内游离钙水平 [Ca2+]i快速且持久的提升,线粒体活性氧(ROS)的积累,及 随后的线粒体功能障碍。[4]通过caspase-8激活和Bid 分裂,功能性caspase-3的表达可增强Staurosporine诱导MCF7细胞死亡。[5] 1 μM Staurosporine处理,只部分抑制IL-3刺激的Bcl2 磷酸化,而完全阻断PKC调节的 Bcl2磷酸化。[6] Staurosporine 诱导人类包皮成纤维细胞AG-1518凋亡,根据溶酶体组织蛋白酶D调节的 细胞色素 c释放和 caspase 激活。[7] 除了激活传统线粒体凋亡通路, Staurosporine触发一种新型内在凋亡通路, 依赖Apaf-1存在时对caspase-9的激活。[8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells NFHPRndEgXSxdH;4bYPDqGG|c3H5 NYDPZ|BiPDhiaB?= MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NHWtNFYh|ryPLh?= NGnENWozOTN6OEG5NS=>
human colon cancer cell line (LoVo cells) MkPLVJJwdGmoZYLheIlwdiCjc4PhfS=> NYXtfpRrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubDDsbY5mKCiOb2\vJINmdGy|KTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:OC5yMEGg{txONg>? MXixNVU6OTVyNR?=
human LoVo cells M3PtdXBzd2yrZnXyZZRqd25iYYPzZZk> MW[0PEB1dyB5MjDo MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyxVn:gZ4VtdHNiYX\0[ZIhPDhidH:gO|IhcHK|IHL5JG1VXCCjc4PhfS=> M1XMVlIzOTh{OUK5
P19 cells MkjlSpVv[3Srb36gZZN{[Xl? M3TzWWlvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NF0xNjByMjFOwG0v MY[xOVc4OTRzOR?=
human BJ cells NHHFTYhEgXSxdH;4bYPDqGG|c3H5 NXT5S|IyPzJiaB?= MlP3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmoh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPhcINmcW5iQV2gZZN{[XluIFnDOVA:OC5yMEKg{txONg>? MnHwNlI6OjFyOEG=
human HT-29 cells MYnGeY5kfGmxbjDhd5NigQ>? NHfPT2ozKGh? M17EZWVn\mWldDDvckBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIIDveIVvfGmjbDDpckBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCyJIhzeyC3c3nu[{BLSy1zIIP0ZYlvcW6pIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfS=> NXS3cnczOjF2MkizO|U>
human A549 cells M1LIc2N6fG:2b4jpZ:Kh[XO|YYm= NIrXepI4OiCq NV;EXox[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBu\XSqb3S= NWP1T5E4OTh2OES3O|U>
human HT-29 cells Mm\ZSpVv[3Srb36gZZN{[Xl? NIXsco9KdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGmwIHj1cYFvKEiWLUK5JINmdGy|IIXzbY5oKEqFMTDkfYUhe3SjaX7pcoch[nliZnz1c5Jme2OnbnPlJJBt[XSnIILlZYRmeiCjc4PhfUwhUUN3ME2yMlUhdk1? MYiyNVUyOzJ7Mx?=
human HT-29 cells MVPGeY5kfGmxbjDhd5NigQ>? Mlr0NkBp NVfwPJVDUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBz\WS3Y4Tpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwh[W[2ZYKgNkBpenNiYomgeZNqdmdiSlOxJJN1[WmwaX7nJIJ6KG[udX;y[ZNk\W6lZTDj[YxtNWKjc3XkJIF{e2G7LDDFR|UxRTJwNjDuUU4> NYf0dWNwOjF7N{OxNFE>
Sf9 cells NXW0[WpETnWwY4Tpc44h[XO|YYm= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHN6cyCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzMEBKSzVyPUOgcm0v MWWxPFgzOzd6NB?=
human HUVEC MkXwVJJwdGmoZYLheIlwdiCjc4PhfS=> MlTKOFghfG9iN{KgbC=> M1jzO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGXWSWMh[W[2ZYKgOFghfG9iN{KgbJJ{KGK7IF3UWEBie3OjeR?= MnXDNlIyQDJ7Mkm=
P19 cells NY[xcFdQTnWwY4Tpc44h[XO|YYm= M{DvcGlvcGmkaYTpc44hd2ZiUILveIVqdiCNaX7hd4UhSSCrbjDQNVkh[2WubIOsJGlEPTB;NDDuUU4> MWSxOVc4OTRzOR?=
Sf9 cells NV7zU5MxTnWwY4Tpc44h[XO|YYm= M4LmcGlvcGmkaYTpc44hd2ZiaIXtZY4hTnmwIHX4dJJme3OnZDDpckBU\jliY3XscJMh[W[2ZYKgNUBucW5iYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOSC3bX;sM2whSVSS MXKxO|MyPTh3Mx?=
Sf21 cells NI\HVGhHfW6ldHnvckBie3OjeR?= MmDWTY5pcWKrdHnvckBw\iCMQVuzJIV5eHKnc4Pl[EBqdiCVZkKxJINmdGy|LDDJR|UxRTZibl2u MUOxO|A5QDB3OR?=
human colon carcinoma cell line HCT116 Mn;rSpVv[3Srb36gZZN{[Xl? NHziO3lEd26lZX70doF1cW:wIILldZVqemWmIH\vdkBoem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBkd2yxbjDjZZJkcW6xbXGgZ4VtdCCuaX7lJGhEXDFzNjygTWM2OD14IH7NMi=> MWSxOVU{PzN2NR?=
human ST486 cells MXTQdo9tcW[ncnH0bY9vKGG|c3H5 M2XBWFQ5KHSxIEeyJIg> NFfhSY1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPUOFg3KGOnbHzzJIFnfGW{IES4JJRwKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PyCwTT6= MVGyNlE5Ojl{OR?=
human MDA-MB-231 cells MVzDfZRwfG:6aXRCpIF{e2G7 M4qwbFczKGh? NWHpVJJlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDt[ZRpd2RuIFfJOVA:Py5zIH7NMi=> M2nvV|E5PDh2N{e1
P19 cells Mo\USpVv[3Srb36gZZN{[Xl? NVK2[3FxUW6qaXLpeIlwdiCxZjDDfYNtcW5vZHXw[Y5l\W62IHvpcoF{\SBzIHnuJHAyQSClZXzsd{whUUN3ME24JI5ONg>? NFHRZm8yPTd5MUSxPS=>
human DLD1 cells MonQVJJwdGmoZYLheIlwdiCjc4PhfS=> M1z1elQ5NTd{IHi= MmfJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGTFSxJINmdGy|IHHmeIVzKDR6IITvJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9PUBvVS5? NWTZXWx2OjJzOEK5Nlk>
insect cells Mn\zSpVv[3Srb36gZZN{[Xl? M{TqXWlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiUHntNUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJIJ6KEiWUl[sJGlEPTB;MUCgcm0v Mnn4NVkyPzlyN{[=
V79 MZ cells NFzPdYtHfW6ldHnvckBie3OjeR?= NFHxNJVKdmirYnn0bY9vKG:oIHj1cYFvKGGuZH;zeIVzd26nIIP5cpRp[XOnIHX4dJJme3OnZDDpckBXPzliTWqgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjbHTvd5Rmem:wZTDzfY51cGW|aYOsJGlEPTB;MUGgcm0v M{PIdlI1PDJ{NUG5
P19 cells NVrtW5Q3TnWwY4Tpc44h[XO|YYm= NYTFVZE5UW6qaXLpeIlwdiCxZjDWZZNkfWyjcjDlcoRwfGinbHnhcEBoem:5dHig[oFkfG:{IILlZ4VxfG:{IHnuJHAyQSClZXzsd{whUUN3ME2xOEBvVS5? NH\CeZcyPTd5MUSxPS=>
Sf9 cells NUjsZ2hKTnWwY4Tpc44h[XO|YYm= NXXPNWE1OjBibXnudy=> MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGZ6diCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIFnfGW{IEKwJI1qdnNiYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOSC3bX;sM2whSVSSLDDJR|UxRTF3IH7NMi=> MkLFNVc{OTV6NUO=
human PBMC NYCybpB5TnWwY4Tpc44h[XO|YYm= M4rJUFI1KGh? M4nRSHN2eHC{ZYPzbY9vKG:oIFnMNkBxem:mdXP0bY9vKGmwIHj1cYFvKFCETVOgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVE3KG6PLh?= NW\tOVVKOTh3OEWwOFY>
human A549 cells NVHP[HpqS3m2b4TvfIlkyqCjc4PhfS=> NWj2emFLPDhiaB?= M{jnRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUAtKEmFNUC9NlAhdk1w MXeyOVgzPTl|NB?=
human CEM cells NUPlbJBNS3m2b4TvfIlkyqCjc4PhfS=> NFLpd3A4OiCq M4TUdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{Bi\nSncjC3NkBpenNiYomgR4Ft[2WrbjDBUUBie3OjeTygTWM2OD1{MzDuUU4> MWGyNlkzOTB6MR?=
human HeLa cells M4TYU2N6fG:2b4jpZ:Kh[XO|YYm= Ml;NOFghcA>? NXzoepJCS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI2KG6PLh?= MUeyOVgzPTl|NB?=
human PC3 cells M4q5[2N6fG:2b4jpZ:Kh[XO|YYm= NITZTlI1QCCq MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTCsJGlEPTB;M{Ggcm0v MW[yOVgzPTl|NB?=
human SF268 cells MUfDfZRwfG:6aXRCpIF{e2G7 MWi0PEBp MmnoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3ME20OEBvVS5? MlvXNlE2OTN{OUS=
human MCF7 cells MUnDfZRwfG:6aXRCpIF{e2G7 M2nXTVQ5KGh? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NUCgcm0v MnP5NlE{QDhzOUG=
HEK293 cells NWDTN4t7S3m2b4TvfIlkyqCjc4PhfS=> NILHPYo4OiCq M3S2eWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{R3zvJIF{e2G7LDDJR|UxRTV4IH7NMi=> M4CwUVI1PzZ|Mk[y
HUE cells Mn\rSpVv[3Srb36gZZN{[Xl? MWm5NEBucW6| M3rHPWlvcGmkaYTpc44hd2ZiVlXHSnIzKGmwIFjVSUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIG\FS2YucW6mdXPl[EBifXSxcHjvd5Bpd3K7bHH0bY9vKHS{ZXH0[YQh\m:{IEmwJI1qdnNiYnXmc5JmKF[HR1[gZ4hidGynbnflJIJ6KEWOSWPBMEBKSzVyPUewJI5ONg>? NEfZdm0zODF5MEG2Ny=>
human A431 cells MVnDfZRwfG:6aXRCpIF{e2G7 MmTWNlQhKGh? NFrzOGhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPDNzIHPlcIx{KGGodHXyJFI1KGi{czD1d4lv\yCDbn7lfIlvKF[nRlnUR{9xem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJ6KE2WVDDhd5NigSxiSVO1NF04OCCwTT6= NYG1PGNEOjJ3NEGwOVE>
human Jurkat cells NX\tNnVUWHKxbHnm[ZJifGmxbjDhd5NigQ>? NGT1dIhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDJUFIue3SrbYXsZZRm\CCqdX3hckBLfXKtYYSgZ4VtdHNuIFnDOVA:PzFibl2u Mmn5NVk1Ojd{MEO=
HEK293 cells MWfGeY5kfGmxbjDhd5NigQ>? NIjodJJKdmirYnn0bY9vKG:oIFnMMVghemWuZXHz[UBjgSCKRVuyPVMh[2WubIOg[ZhxemW|c3nu[{BRU0NvYnX0ZVItKEmFNUC9O|chdk1w NGfqS3AyPTd5MUSxPS=>
human KE-97 cells NHHCR5BEgXSxdH;4bYPDqGG|c3H5 MXe3NkBp MofMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2UuQTdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yOyEQvF2u NH3TS|kzPDN{OEK4Ny=>
human CHOK1 cells MXnDfZRwfG:6aXRCpIF{e2G7 MVq0PEBp MlXTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2hQUzFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4Phfg+9lCCLQ{WwQVAvOTNizszNMi=> M1vWVVIyPTF|Mkm0
mouse NIH/3T3 cells NVz5OIlVS3m2b4TvfIlkyqCjc4PhfS=> NWjxT|lbQTZiaB?= MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDOTWgwO1R|IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NE4zKM7:TT6= Mk\ENlQ{PjF3MkG=
human A2780 cells MVXDfZRwfG:6aXRCpIF{e2G7 Ml3NPVYhcA>? MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuNkDPxE1w Mon0NlQ{PjF3MkG=
human 8505C cells Mn3DR5l1d3SxeHnjxsBie3OjeR?= MYK5OkBp MnTDR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gPFUxPUNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIh|ryPLh?= MXWyOFM3OTV{MR?=
human 518A2 cells MUDDfZRwfG:6aXRCpIF{e2G7 MkjOPVYhcA>? MoLsR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gOVE5STJiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4Phfg+9lCCLQ{WwQVAvOiEQvF2u MkiwNlQ{PjF3MkG=
human HuH7 cells NV;Gc2x6S3m2b4TvfIlkyqCjc4PhfS=> NHrEO2w4OiCq M2nPXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zOyEQvF2u NXHwXFVQOjR|MkiyPFM>
FL5.12-Akt1 cells NWDRSJVjWHKxbHnm[ZJifGmxbjDhd5NigQ>? MlPQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDGUFUvOTJvQXv0NUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NE4zQSEQvF2u MWixOlQxOzZ{Nh?=
human MiaPaCa-2 cells NX7pS3RsWHKxbHnm[ZJifGmxbjDhd5NigQ>? MlrHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPaXHQZWNiNTJiY3XscJMtKEmFNUC9NE4{PyEQvF2u MVixOlQyOzd6MB?=
human BGC823 cells MmfMR5l1d3SxeHnjxsBie3OjeR?= NE\UVGc4OiCq NF\aSG9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRz\S2JbH:gcJVucW6nc3PlcpQh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|ODFOwG0v NGSxboUzPDN{OEK4Ny=>
human MCF7 cells NV3hWVVuS3m2b4TvfIlkyqCjc4PhfS=> MVq5OkBp NVf2d41{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvPCEQvF2u Mkn2NlQ{PjF3MkG=
human A549 cells NEDYdmtEgXSxdH;4bYPDqGG|c3H5 NIXIZ4k6PiCq M2naR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlYh|ryPLh?= NGOwSlAzPDN4MUWyNS=>
HEK293 cells NXfyW2g3S3m2b4TvfIlkyqCjc4PhfS=> NHn2U3REgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOsJGVEPTB;MjFOwG0v Mn;BNlU{OTZ|MUe=
human Raji cells  MYDDfZRwfG:6aXRCpIF{e2G7 Mm[3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{whTUN3ME2yJO69VS5? NXzmTotYOjV|MU[zNVc>
human HepG2 cells NIrSbGtEgXSxdH;4bYPDqGG|c3H5 M2OwWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{NCCHQ{WwQVIh|ryPLh?= NXXob3ZJOjV|MU[zNVc>
human BJ cells MUXDfZRwfG:6aXRCpIF{e2G7 NIDPW|VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDUiClZXzsd{whTUN3ME2yJO69VS5? NUXLUIp4OjV|MU[zNVc>
human U937 cells NXjmOm9wS3m2b4TvfIlkyqCjc4PhfS=> M4nVS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHU6OzdiY3XscJMtKEmFNUC9NkDPxE1w NHW1OYwyPzB6OEC2Oy=>

... Click to View More Cell Line Experimental Data

体内研究 在局部贫血前,使用Staurosporine(0.1-10 ng)预处理局部贫血沙鼠和大鼠模型,可防止神经元损伤,这种作用存在剂量依赖性,说明CAl锥体细胞死亡涉及PKC。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

+ 展开

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
细胞实验:[4]
+ 展开
  • Cell lines: PC12
  • Concentrations: 溶于DMSO,终浓度为1 μM
  • Incubation Time: ~32 小时
  • Method: 使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合,然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞,测定凋亡细胞百分数。
    (Only for Reference)
动物实验:[2]
+ 展开
  • Animal Models: 短暂局部贫血的雄性Mongolian沙鼠或雄性 Wistar大鼠
  • Formulation: 溶于DMSO,然后在盐水中稀释
  • Dosages: ~10 ng
  • Administration: 定向处理到海马双边CA1子域
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 466.53
化学式

C28H26N4O3

CAS号 62996-74-1
稳定性 powder
in solvent
别名 CGP 41251

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00082017 Terminated Lymphoma Large-Cell Ki-1|Lymphoma T-Cell National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) April 5 2004 Phase 2
NCT00301938 Completed Accelerated Phase Chronic Myelogenous Leukemia|Adult Acute Megakaryoblastic Leukemia (M7)|Adult Acute Minimally Differentiated Myeloid Leukemia (M0)|Adult Acute Monoblastic Leukemia (M5a)|Adult Acute Monocytic Leukemia (M5b)|Adult Acute Myeloblastic Leukemia With Maturation (M2)|Adult Acute Myeloblastic Leukemia Without Maturation (M1)|Adult Acute Myeloid Leukemia With 11q23 (MLL) Abnormalities|Adult Acute Myeloid Leukemia With Inv(16)(p13;q22)|Adult Acute Myeloid Leukemia With t(15;17)(q22;q12)|Adult Acute Myeloid Leukemia With t(16;16)(p13;q22)|Adult Acute Myeloid Leukemia With t(8;21)(q22;q22)|Adult Acute Myelomonocytic Leukemia (M4)|Adult Acute Promyelocytic Leukemia (M3)|Adult Erythroleukemia (M6a)|Adult Pure Erythroid Leukemia (M6b)|Blastic Phase Chronic Myelogenous Leukemia|Myelodysplastic/Myeloproliferative Neoplasms|Previously Treated Myelodysplastic Syndromes|Recurrent Adult Acute Lymphoblastic Leukemia|Recurrent Adult Acute Myeloid Leukemia|Relapsing Chronic Myelogenous Leukemia|Secondary Acute Myeloid Leukemia|T-cell Adult Acute Lymphoblastic Leukemia|Untreated Adult Acute Lymphoblastic Leukemia|Untreated Adult Acute Myeloid Leukemia National Cancer Institute (NCI) December 2005 Phase 1
NCT00098956 Completed Extensive Stage Small Cell Lung Cancer|Recurrent Small Cell Lung Cancer National Cancer Institute (NCI) January 2005 Phase 2
NCT00072267 Completed Fallopian Tube Cancer|Ovarian Cancer|Primary Peritoneal Cavity Cancer University Health Network Toronto|National Cancer Institute (NCI) January 2004 Phase 2
NCT00072189 Terminated Recurrent Melanoma|Stage IV Melanoma National Cancer Institute (NCI) November 2003 Phase 2
NCT00030888 Unknown status Kidney Cancer University of California San Francisco|National Cancer Institute (NCI) December 2002 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

PKC Signaling Pathway Map

相关PKC产品

Tags: 购买Staurosporine | Staurosporine供应商 | 采购Staurosporine | Staurosporine价格 | Staurosporine生产 | 订购Staurosporine | Staurosporine代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID