Histone H3.3 Rabbit Recombinant mAb
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使用信息
| 抗体应用 | wb,ELISA | ||
|---|---|---|---|
| 稀释比例 |
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| 反应性 | Human Mouse Rat | ||
| MW (kDa) | 15kDa | ||
| 抗体类型 | Rabbit | ||
| 浓度 | 1mg/ml | ||
| 储存液配方 | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| 储存条件 (自收到货起) |
Store at –20°C. |
生物描述
| 特异性 | Histone H3.3 Rabbit Recombinant mAb detects endogenous levels of total Histone H3.3. |
|---|---|
| 背景 | Histone H3.3 is a conserved histone variant structurally very close to the canonical histone H3 and has been associated with active transcription. H3.3 differs from the canonical replication-coupled H3 histones (H3.1/H3.2 in mammals) by only four to five amino acids, respectively, but has distinct expression patterns and chromatin incorporation mechanisms. Canonical H3 variants are expressed only during S phase, and their incorporation is mediated by the CAF-1 chromatin assembly complex. In contrast, H3.3 is expressed throughout the cell cycle and is incorporated via a number of different pathways, including the CHD1 and ATRX chromatin remodeling complexes and the HIRA chaperone complex. H3.3 is enriched in transcribed genes, enhancers, regulatory elements, and also heterochromatic repeats, including telomeres and pericentromeric regions. It has been implicated in the epigenetic regulation of diverse biological processes. When incorporated into a nucleosome, H3.3 accumulates modifications associated with gene activation or open chromatin—including methylation on K4, K36, and K79 and acetylation on K9 and K14—and is deprived of suppressive modifications, including K9 and K27 methylations. H3.3 is considered as a mark of transcriptional activity. |
实验方法
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