Histone H3 (mono methyl K36) Rabbit Recombinant mAb

目录号：A5223

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20ul	RMB 447.01	现货
100ul	RMB 1500.43	现货
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使用信息

抗体应用

IF,ELISA

稀释比例

1:50

反应性

Human Mouse

MW (kDa)

15kDa

抗体类型

Rabbit

浓度

1mg/ml

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

Datasheet & SDS

生物描述

特异性	Histone H3 (mono methyl K36) Rabbit Recombinant mAb detects endogenous level of total Histone H3 (mono methyl K36).
背景	Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4. In the case of lysine, this includes the formation of mono-, di-, or trimethyl groups, each of which is presumed to represent a distinct functional state at the cellular level. Numerous HMTases have recently been characterized and are thought to catalyze the addition of methyl groups onto the amino terminal tails of histones H3 (K4, K9, K27, K36) and H4 (K20). Histone H3 K36 methylation is found in a broad range of distinct eukaryotes and is highly conserved. K36 methylation may have a broad range of activities in chromatin in addition to a conserved role with the transcribing polymerase.

特异性

Histone H3 (mono methyl K36) Rabbit Recombinant mAb detects endogenous level of total Histone H3 (mono methyl K36).

背景

Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4. In the case of lysine, this includes the formation of mono-, di-, or trimethyl groups, each of which is presumed to represent a distinct functional state at the cellular level. Numerous HMTases have recently been characterized and are thought to catalyze the addition of methyl groups onto the amino terminal tails of histones H3 (K4, K9, K27, K36) and H4 (K20). Histone H3 K36 methylation is found in a broad range of distinct eukaryotes and is highly conserved. K36 methylation may have a broad range of activities in chromatin in addition to a conserved role with the transcribing polymerase.

实验方法

Immunofluorescence (Immunocytochemistry)

Specimen Preparation (forcultured cell lines, IF-IC)

1. Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
2. Fix cells for 15 min at room temperature.
3. Aspirate fixative, rinse three times in 1X PBS for 5 min each.
4. Proceed with Immunostaining.

Immunostaining

1. Add theblocking buffer and incubate for 60 min at RT.
2. Prepare primary antibody diluent in antibody dilution buffer as recommended .
3. Aspirate blocking solution, apply diluted primary antibody.
4. Incubate overnight at 4°C.
5. Rinse three times in 1X PBS for 5 min each.
6. Incubate specimens in fluorochrome-conjugated secondary antibody diluted in antibody dilution buffer for 1–2 hr at room temperature in the dark.
7. Rinse three times in 1X PBS for 5 min each.
8. Mount slides usingmounting medium with DAPI and cover with coverslips.
9. For best results, allow mountant to cure overnight at room temperature. For long-term storage, store slides flat at 4°C protected from light.

参考文献	[1] Vincent L.Biron, et al. Developmental Biology. 2004, 276(2)：337-351. [2] Stephanie A. Morris, et al. Eukaryot Cell. 2005, 4(8): 1446–1454.

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