Adavosertib (MK-1775)

For research use only. Not for use in humans.

目录号：S1525 别名： AZD1775

Adavosertib (MK-1775) Chemical Structure

CAS No. 955365-80-7

Adavosertib (MK-1775, AZD1775)是一种有效的，高选择性Wee1抑制剂，无细胞试验中IC50为5.2 nM；阻碍G2期DNA损伤检验点。Phase 2。

规格

价格

库存

购买数量

10mM (1mL in DMSO)	RMB 1960.96	现货
5mg	RMB 1376.38	现货
10mg	RMB 2226.67	现货
25mg	RMB 3835.28	现货
50mg	RMB 6297.29	现货
100mg	RMB 9582.3	现货
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客户使用Selleck生产的Adavosertib (MK-1775)发表文献128篇：

Cancer Cell, 2020, 15 pii: S1535-6108(20)30164-1

PubMed: 32359397 ( click the link to review the publication )

Nat Med, 2019, 25(5):838-849

PubMed: 31011202 ( click the link to review the publication )

Cancer Cell, 2019, 35(3):519-533

PubMed: 30889383 ( click the link to review the publication )

Cancer Cell, 2019, 35(5):752-766

PubMed: 31085176 ( click the link to review the publication )

Cell, 2018, 173(1):104-116

PubMed: 29502971 ( click the link to review the publication )

Cell, 2013, 155(5):1088-103

PubMed: 24267891 ( click the link to review the publication )

Sci Adv, 2020, 6(24):eaaz7808

PubMed: 32577513 ( click the link to review the publication )

Clin Cancer Res, 2020, 27 pii: clincanres

PubMed: 32341033 ( click the link to review the publication )

Haematologica, 2020, 12;haematol.2019.233445

PubMed: 32165486 ( click the link to review the publication )

Cancer Lett, 2020, 472:119-131

PubMed: 31866466 ( click the link to review the publication )

Br J Haematol, 2020, 21

PubMed: 32314355 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(1):148-163

PubMed: 32064158 ( click the link to review the publication )

Am J Cancer Res, 2020, 1;10(3):908-924

PubMed: 32266099 ( click the link to review the publication )

Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0507

PubMed: 32229503 ( click the link to review the publication )

J Cell Mol Med, 2020, 27

PubMed: 32459381 ( click the link to review the publication )

Int J Mol Sci, 2020, 18;21(8):2825

PubMed: 32325639 ( click the link to review the publication )

Cancer Cell Int, 2020, 20:253

PubMed: 32565737 ( click the link to review the publication )

Ther Adv Hematol, 2020, 11:2040620719898373

PubMed: 32010435 ( click the link to review the publication )

Diagnostics (Basel), 2020, 10(2)

PubMed: 32098452 ( click the link to review the publication )

Cell Rep, 2020, 32(2):107901

PubMed: 32668239 ( click the link to review the publication )

JCI Insight, 2020, 143474

PubMed: 33320833 ( click the link to review the publication )

J Neurooncol, 2020, 10.1007

PubMed: 32180106 ( click the link to review the publication )

Cancers (Basel), 2020, 12(6):1503

PubMed: 32526907 ( click the link to review the publication )

FEBS J, 2020, 10.1111/febs.15280

PubMed: 32144855 ( click the link to review the publication )
Nat Commun, 2019, 10(1):2189

PubMed: 31097698 ( click the link to review the publication )

Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-19-0775

PubMed: 31806643 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(11):3430-3442

PubMed: 30755439 ( click the link to review the publication )

Leukemia, 2019, 10.1038/s41375-019-0456-2

PubMed: 30967619 ( click the link to review the publication )

Haematologica, 2019, 104(11):2225-2240

PubMed: 30819918 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(7):2290-2304

PubMed: 30538111 ( click the link to review the publication )

J Cell Biol, 2019, 218(5):1531-1552

PubMed: 30858191 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-18-3374

PubMed: 31551363 ( click the link to review the publication )

Elife, 2019, e46902

PubMed: 31264963 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):463

PubMed: 31718704 ( click the link to review the publication )

Cancers (Basel), 2019, 11(11)

PubMed: 31703356 ( click the link to review the publication )

Cancers (Basel), 2019, 11(6)

PubMed: 31200459 ( click the link to review the publication )

Cells, 2019, 8(2)

PubMed: 30754676 ( click the link to review the publication )

Cell Chem Biol, 2019, 10.1016/j.chembiol.2019.10.013

PubMed: 31735695 ( click the link to review the publication )

Oncoimmunology, 2019, 8(11):e1638207

PubMed: 31646086 ( click the link to review the publication )

Mol Cancer Ther, 2019, 18(8):1439-1450

PubMed: 31142662 ( click the link to review the publication )

Mol Cancer Ther, 2019, 10.1158/1535-7163.MCT-19-0474

PubMed: 31597711 ( click the link to review the publication )

Gastric Cancer, 2019, 10.1007/s10120-019-00977-1

PubMed: 31197522 ( click the link to review the publication )

Hum Mol Genet, 2019, 28(5):842-857

PubMed: 30445628 ( click the link to review the publication )

Eur J Med Chem, 2019, 161:479-492

PubMed: 30388464 ( click the link to review the publication )

J Virol, 2019, 93(14)

PubMed: 31043526 ( click the link to review the publication )

Sci Rep, 2019,

PubMed: 31848373 ( click the link to review the publication )

Sci Rep, 2019, 9(1):6161

PubMed: 30992462 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1942-1954

PubMed: 31523195 ( click the link to review the publication )
Front Pharmacol, 2019, 10:864

PubMed: 31427973 ( click the link to review the publication )

Exp Cell Res, 2019, 111720

PubMed: 31738907 ( click the link to review the publication )

PLoS One, 2019, 14(2):e0212210

PubMed: 30779812 ( click the link to review the publication )

PLoS One, 2019, 14(11):e0221288

PubMed: 31721781 ( click the link to review the publication )

Biochem Biophys Res Commun, 2019, 519(3):597-604

PubMed: 31540690 ( click the link to review the publication )

Biochem Biophys Res Commun, 2019, 10.1016/j.bbrc.2019.10.124

PubMed: 31640856 ( click the link to review the publication )

Blood Adv, 2019, 3(7):1185-1196

PubMed: 30967394 ( click the link to review the publication )

Leukemia, 2019, 10.1038/s41375-019-0677-4

PubMed: 31836849 ( click the link to review the publication )

Nat Commun, 2018, 9(1):3982

PubMed: 30266942 ( click the link to review the publication )

Nucleic Acids Res, 2018, 46(10):5109-5124

PubMed: 29850896 ( click the link to review the publication )

J Immunother Cancer, 2018, 6(1):59

PubMed: 29925431 ( click the link to review the publication )

Cancer Res, 2018, 78(11):3054-3066

PubMed: 29735549 ( click the link to review the publication )

Cell Rep, 2018, 22(12):3206-3216

PubMed: 29562177 ( click the link to review the publication )

Cancer Lett, 2018, 430:34-46

PubMed: 29753759 ( click the link to review the publication )

J Exp Clin Cancer Res, 2018, 37(1):129

PubMed: 29954437 ( click the link to review the publication )

Cancers (Basel), 2018, 10(5)

PubMed: 29783721 ( click the link to review the publication )

Curr Biol, 2018, 28(23):3824-3832

PubMed: 30449668 ( click the link to review the publication )

Cells, 2018, 7(9)

PubMed: 30200644 ( click the link to review the publication )

Front Oncol, 2018, 8:143

PubMed: 29774202 ( click the link to review the publication )

Sci Rep, 2018, 8(1):948

PubMed: 29343688 ( click the link to review the publication )

Int J Biol Sci, 2018, 14(10):1221-1231

PubMed: 30123071 ( click the link to review the publication )

BMC Cancer, 2018, 18(1):922

PubMed: 30253737 ( click the link to review the publication )

Bioorg Med Chem, 2018, 26(14):4014-4024

PubMed: 29941193 ( click the link to review the publication )

PLoS One, 2018, 13(2):e0193098

PubMed: 29489886 ( click the link to review the publication )
Biomed Res Int, 2018, 2018:5813292

PubMed: 29977914 ( click the link to review the publication )

Oncol Lett, 2018, 16(4):5473-5481

PubMed: 30250620 ( click the link to review the publication )

Blood, 2017, 130(24):2631-2641

PubMed: 29018079 ( click the link to review the publication )

Genes Dev, 2017, 31(3):318-332

PubMed: 28242626 ( click the link to review the publication )

Aging Cell, 2017, 16(3):575-584

PubMed: 28345297 ( click the link to review the publication )

J Hematol Oncol, 2017, 10(1):77

PubMed: 28356161 ( click the link to review the publication )

J Med Chem, 2017, 60(18):7863-7875

PubMed: 28792760 ( click the link to review the publication )

Br J Cancer, 2017, 117(1):113-123

PubMed: 28535155 ( click the link to review the publication )

Mol Cancer Ther, 2017, 16(9):2022-2034

PubMed: 28619759 ( click the link to review the publication )

J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1044-1045:70-76

PubMed: 28088043 ( click the link to review the publication )

Sci Rep, 2017, 7:43517

PubMed: 28262781 ( click the link to review the publication )

Oncotarget, 2017, 8(43):73705-73722

PubMed: 29088738 ( click the link to review the publication )

Oncotarget, 2017, 8(7):10966-10979

PubMed: 28030798 ( click the link to review the publication )

Oncotarget, 2017, 8(63):106352-106368

PubMed: 29290954 ( click the link to review the publication )

Oncotarget, 2017, 8(55):94780-94792

PubMed: 29212266 ( click the link to review the publication )

Oncotarget, 2017, 8(27):44295-44311

PubMed: 28574828 ( click the link to review the publication )

Oncotarget, 2017, 8(67):111026-111040

PubMed: 29340034 ( click the link to review the publication )

Oncotarget, 2017, 8(35):59476-59491

PubMed: 28938651 ( click the link to review the publication )

Clin Cancer Res, 2016, 22(17):4440-51

PubMed: 27103402 ( click the link to review the publication )

Stem Cells, 2016, 34(7):1765-75

PubMed: 26946228 ( click the link to review the publication )

Mol Cancer Ther, 2016, 15(7):1669-81

PubMed: 27196765 ( click the link to review the publication )

Mol Cancer Ther, 2016, 15(6):1332-43

PubMed: 27196784 ( click the link to review the publication )

J Cell Sci, 2016, 129(16):3167-77

PubMed: 27383768 ( click the link to review the publication )

Sci Rep, 2016, 6:33323

PubMed: 27616351 ( click the link to review the publication )
BMC Cancer, 2016, 16:678

PubMed: 27558154 ( click the link to review the publication )

Oncotarget, 2016, 7(27):41320-41335

PubMed: 27191498 ( click the link to review the publication )

Oncotarget, 2016, 7(31):49902-49916

PubMed: 27363019 ( click the link to review the publication )

Oncotarget, 2016, 7(23):34785-99

PubMed: 27166183 ( click the link to review the publication )

Genetics, 2016, 204(2):807-819

PubMed: 27558135 ( click the link to review the publication )

Tumour Biol, 2016, 10.1007/s13277-016-5081-3

PubMed: 27220319 ( click the link to review the publication )

Genes Dev, 2015, 29(5):555-66

PubMed: 25737283 ( click the link to review the publication )

Sci Rep, 2015, 5:16991

PubMed: 26601688 ( click the link to review the publication )

Cancer Biol Ther, 2015, 16(12):1784-93

PubMed: 26529495 ( click the link to review the publication )

Cell Cycle, 2015, 14(7):1070-81

PubMed: 25602147 ( click the link to review the publication )

BMC Cancer, 2015, 15:462

PubMed: 26054341 ( click the link to review the publication )

Pediatr Blood Cancer, 2015, 62(1):52-9

PubMed: 25308916 ( click the link to review the publication )

Radiat Res, 2015, 184(6):660-9

PubMed: 26645158 ( click the link to review the publication )

Oncotarget, 2015, 6(15):13072-87

PubMed: 25965828 ( click the link to review the publication )

Genes Cancer, 2015, 6(5-6):184-213

PubMed: 26124919 ( click the link to review the publication )

Oncotarget, 2015, 6(25):21074-84

PubMed: 26025928 ( click the link to review the publication )

BMC Cancer, 2015, 15:462

PubMed: 26054341 ( click the link to review the publication )

J Thorac Oncol, 2014, 9(7):965-73

PubMed: 24922006 ( click the link to review the publication )

Genes Dev, 2014, 28(14):1578-91

PubMed: 25030697 ( click the link to review the publication )

Clin Cancer Res, 2014, 20(16):4274-88

PubMed: 25125259 ( click the link to review the publication )

J Cell Biol, 2014, 206(7):843-53

PubMed: 25246615 ( click the link to review the publication )

J Hematol Oncol, 2014, 7(1):53

PubMed: 25084614 ( click the link to review the publication )

Cancer Lett, 2014, 356(2 Pt B):656-68

PubMed: 25458954 ( click the link to review the publication )

J Cell Mol Med, 2014, 18(1):143-55

PubMed: 24238094 ( click the link to review the publication )
Pediatr Blood Cancer, 2014, 61(10):1767-73

PubMed: 24962331 ( click the link to review the publication )

Oncotarget, 2014, 5(21):10546-57

PubMed: 25301733 ( click the link to review the publication )

Cell, 2014, 157(3):565-79

PubMed: 24766806 ( click the link to review the publication )

Expert Opin Drug Metab Toxicol, 2013, 9(2):215-24

PubMed: 23320990 ( click the link to review the publication )

PLoS One, 2013, 8(3):e57523

PubMed: 23520471 ( click the link to review the publication )

Obstet Gynecol Int, 2013, 2013:828165

PubMed: 24381593 ( click the link to review the publication )

Mol Cancer Ther, 2012, 11(1):174-82

PubMed: 22084170 ( click the link to review the publication )

Pediatr Surg Int, 2012, 28(6):579-89

PubMed: 22526548 ( click the link to review the publication )

产品安全说明书

Wee1抑制剂选择性比较

生物活性

产品描述

Adavosertib (MK-1775, AZD1775)是一种有效的，高选择性Wee1抑制剂，无细胞试验中IC50为5.2 nM；阻碍G2期DNA损伤检验点。Phase 2。

特性

首个报道的 Wee1抑制剂。

靶点

Wee1 ^[1]
(Cell-free assay)

5.2 nM

体外研究

MK-1775以ATP竞争的方式抑制Wee1激酶。与作用于Wee1相比，MK-1775对Yes的效能低2-到3-倍，IC50为14 nM，对7种其他激酶的效能低10倍，1 μM浓度下抑制率>80%，比对人Myt 1选择性高100倍以上，并抑制周期蛋白依赖性激酶1 (CDC2) 在可选择位点(Thr14) 的磷酸化作用。通过阻断负荷突变型p53的WiDr细胞中Wee1活性，废止DNA损伤检控点，MK-1775治疗抑制Tyr15 (CDC2Y15)位点的CDC2基础磷酸化作用，EC50为49 nM，并剂量依赖性抑制gemcitabine-，carboplatin- 或cisplatin-诱导的CDC2磷酸化和细胞周期阻滞，EC50分别为82 nM和81 nM，180 nM 和163 nM，以及159 nM 和 160 nM。30-100 nM 的MK-1775单独治疗在WiDr 和 H1299细胞中没有显著的抗增殖作用，而300 nM的MK-1775足以抑制>80%的Wee1，表现出温和但显著的抗增殖作用，在WiDr和H1299细胞中抑制率分别为34.1%和28.4%。^[1]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
ASPC-1	M1qxfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MVvJR\|UxRTF\|LkKgxtEhOS5zIN88US=>	M4rPWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
BxPC-3	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NX;kTXBjUUN3ME2wMlghyrFiMD6wN{DPxE1?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR3OEm1OEc,OjV2NUi5OVQ9N2F-
CFPAC-1	Ml\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Mkn1TWM2OD1\|LkOgxtEhOC5{IN88US=>	NYfBboY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OVg6PTRpPkK1OFU5QTV2PD;hQi=>
HPAC	NVX1VG95T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXXJR\|UxRTBwNTFCtUAxNjBzIN88US=>	M{LiVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
MIAPaCa-2	NGjpbmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVrJR\|UxRTBwNTFCtUAxNjB3IN88US=>	M13QV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
PANC-1	M4f0OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M120dWlEPTB;MUCuOkDDuSBzLkGg{txO	MnPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NUi5OVQoRjJ3NEW4PVU1RC:jPh?=
SK-N-BE (2)	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWHJR\|UxRTJwNPMAjeKy6oDLMD6zJO69VQ>?	NHr3R4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), PAN→MK	NUi0PGJGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MYDJR\|UxRTJ4LkdihKnDueLCiUmuOkDPxE1?	NUnoZ\|VnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N\|A5QTF4PD;hQi=>
SK-N-BE (2), MK→PAN	NHn1[\|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M4j2OGlEPTB;Mj605qCKyrIkgJmwMlMh\|ryP	M1v0O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-AS	NXvs[5FtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NV\YOHlMUUN3ME2wMlUx6oDLwsJihKkxNjB{IN88US=>	M160OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-DZ	M1jF[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MVjJR\|UxRTBwM{dihKnDueLCiUCuNFEh\|ryP	MnvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-AS	NI[yfHVCeG:ydH;zbZMhSXO\|YYm=	NFKzNYM2ODBibl2=	NYL6b4t3PDhiaB?=	NVvDPGZUcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	M1LZVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-DZ	MYfBdI9xfG:\|aYOgRZN{[Xl?	MWW1NFAhdk1?	NX\XNGV[PDhiaB?=	NGrv[5BqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	NGHLWHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
THP-1	NVTNdGdoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoPwNVI2NzJ3MD:1NFAhdk1?	NEnDNmg1QCCq	MlO3bY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	Mon5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOES2NVQoRjJ3MEi0OlE1RC:jPh?=
MV4-11	M2O0UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFz2[\|cyOjVxMkWwM\|UxOCCwTR?=	NX\Y[3JPPDhiaB?=	NIjBVIhqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>?	NFXjWFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OFYyPCd-MkWwPFQ3OTR:L3G+
U937	NXvL[nZJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHjJWJMyOjVxMkWwM\|UxOCCwTR?=	NIjISFM1QCCq	M{TsSolv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz	M3\EflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEi0OlE1Lz5{NUC4OFYyPDxxYU6=
HL-60	NW\yWZFZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFPTeY4yOjVxMkWwM\|UxOCCwTR?=	Mnz2OFghcA>?	MoHWbY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB6NE[xOEc,OjVyOES2NVQ9N2F-
OCI-AML3	Mm\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkGxNVI2NzJ3MD:1NFAhdk1?	MVS0PEBp	NIewVldqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>?	M4LEdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEi0OlE1Lz5{NUC4OFYyPDxxYU6=
MOLM-13	MkS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGi2TVQyOjVxMkWwM\|UxOCCwTR?=	Mn\hOFghcA>?	M37vfYlv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz	NYW4UldmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPFQ3OTRpPkK1NFg1PjF2PD;hQi=>
CMK	NGfzbW5E\WyuIG\pZYJqdGm2eTDBd5NigQ>?	NIS2PFIyOC1zMECwNEBvVQ>?	M4G4UFczKGh?	M1:2SZJm\HWlZYOgZ4VtdCC4aXHsbYJqfHliaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDtZY5v\XJ?	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MkOzNUc,OjR7NkKzN\|E9N2F-
CMY	Mk\tR4VtdCCYaXHibYxqfHliQYPzZZk>	MXWxNE0yODByMDDuUS=>	M{XoZVczKGh?	MWfy[YR2[2W\|IHPlcIwhfmmjbHnibZR6KGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz	Mnr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkKzN\|EoRjJ2OU[yN\|MyRC:jPh?=
Dayo	NIXMT29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NHzWS21KSzVyPUG1NEBvVQ>?	Mo\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NkG5NVAoRjJ2Nk[xPVExRC:jPh?=
UW228	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NUixVYpbUUN3ME2yN\|Ihdk1?	NXXFOJAxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2OlE6OTBpPkK0OlYyQTFyPD;hQi=>
IST-MES1	MUHD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	NYT5e3p5OTVyL{K1NEBvVQ>?	NHLVcoQ4OiCq	NX6zSWtN\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	NHzRRXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OVc5Oid-MkSzOlU4QDJ:L3G+
IST-MES2	MUDD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	Mn;4NVUxNzJ3MDDuUS=>	NWHOOJdDPzJiaB?=	NVjnVINy\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	M3zuelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[1O\|gzLz5{NEO2OVc5OjxxYU6=
REN	MUDD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	Ml3aNVUxNzJ3MDDuUS=>	NFexVHI4OiCq	MXvlcohidmOnczD0bIUh[2m\|cHzheIlvKGO7dH;0c5hq[yCnZn\lZ5QhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NIHNe3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OVc5Oid-MkSzOlU4QDJ:L3G+
NCI-H2452	MWXD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	MX2xOVAwOjVyIH7N	NHjldmg4OiCq	MYPlcohidmOnczD0bIUh[2m\|cHzheIlvKGO7dH;0c5hq[yCnZn\lZ5QhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NGC1RpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OVc5Oid-MkSzOlU4QDJ:L3G+
MSTO-211H	NU[3emsyS2WubDDWbYFjcWyrdImgRZN{[Xl?	M1;VeFE2OC9{NUCgcm0>	NEDSVlE4OiCq	NYnhVJBk\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	NGflb2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OVc5Oid-MkSzOlU4QDJ:L3G+
NCI-H2052	M1SxfWNmdGxiVnnhZoltcXS7IFHzd4F6	NFjKT5YyPTBxMkWwJI5O	NYHjPHpCPzJiaB?=	NHrwZY5mdmijbnPld{B1cGViY3nzdIxifGmwIHP5eI91d3irYzDl[oZm[3RiaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDtZY5v\XJ?	NXXMWmY1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
WEE1	NWC3OJJoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1v6[2lEPTB;NT6yJI5O	M1ixSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Nkm5OlU2Lz5{M{[5PVY2PTxxYU6=
CDC2	NHux[IVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M{mxcmlEPTExvK6xNFAxKG6P	Mmi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OUm2OVUoRjJ\|Nkm5OlU2RC:jPh?=
CDK7	NVfyOlFzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MmHmTWM2OO,:nkGwNFAhdk1?	NVnsO40yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PVk3PTVpPkKzOlk6PjV3PD;hQi=>
MYT1	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3;5NWlEPTB;NUOwJI5O	M335fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Nkm5OlU2Lz5{M{[5PVY2PTxxYU6=
T98G	MUPBdI9xfG:\|aYOgRZN{[Xl?	MV[xNFAwOjVyIH7N	M{DtXFYhcA>?	M4DvZYVvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxia3nscIlv\w>?	MlL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OUK3PVMoRjJzOUmyO\|k{RC:jPh?=
A549	Mn7FRZBweHSxc3nzJGF{e2G7	NY\hepRTOjByIH7N	Mm\hNUBp	NVzNcVhFemGmaX;z[Y5{cXSrenXzJG5US0yFIHPlcIx{KGmwIHGgdFU{NWSncHXu[IVvfCCvYX7u[ZI>	NITndVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe5PVA{Oyd-MkG3PVkxOzN:L3G+
H460	Mn7IRZBweHSxc3nzJGF{e2G7	M3nsU\|IxOCCwTR?=	NU\Nc3ExOSCq	MXXyZYRqd3OnboPpeIl7\XNiTmPDUGMh[2WubIOgbY4h[SCyNUOt[IVx\W6mZX70JI1idm6nch?=	MmXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5OUmwN\|MoRjJzN{m5NFM{RC:jPh?=
H1299	NV:ySm92SXCxcITvd4l{KEG\|c3H5	MXOyNFAhdk1?	MoLMNUBp	MoTsdoFlcW:\|ZX7zbZRqgmW\|IF7TR2xEKGOnbHzzJIlvKGFicEWzMYRmeGWwZHXueEBu[W6wZYK=	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd7OUCzN{c,OjF5OUmwN\|M9N2F-
Calu-6	MUfBdI9xfG:\|aYOgRZN{[Xl?	NX\KeYRZOjByIH7N	MWGxJIg>	NFq5VmVz[WSrb4PlcpNqfGm8ZYOgUnNEVENiY3XscJMhcW5iYTDwOVMu\GWyZX7k[Y51KG2jbn7ldi=>	NYfnfmNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3PVkxOzNpPkKxO\|k6ODN\|PD;hQi=>
WiDr	MoHKT4lv[XOnIFHzd4F6ew>?	MXuxNE0yODByMDDuUS=>	MnfKPEBp	Mmi4bY5pcWKrdIOgdIhwe3Cqb4L5cIF1cW:wIH;mJGNFSzJiYYSgWJlzOTVid3n0bEBidiCHQ{WwxsB3[Wy3ZTDv[kA5PSCwbX;sM2wheHKndILlZZRm\CC5aYToJIdmdWOrdHHibY5m	NVjiSVhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PFc2PDVpPkG5PFg4PTR3PD;hQi=>
Function assay	MVLFfJBqOjl\|Rh?=			NFXkUXpDcW6maX7nJIFn\mmwaYT5JJRwKHKnY3;tZolv[W62IHj1cYFvKG[3bHytcIVv\3SqIF6teIVzdWmwYXygTIl{QC22YXfn[YQhX2WnMTCoNUB1dyB4NE[gdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCqdX3hckBGgHCrMkmzSkBk\WyuczDhd5Nme3OnZDDhd{Bl\XO\|b3PpZZRqd25iY3;ud5RidnRiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:mLDDL[EA:KDBwMECzNkDPxE1w	M1i5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Function assay	NFKyOlNGgHCrMkmzSi=>			NHS1W4ZDcW6maX7nJIFn\mmwaYT5JJRwKHKnY3;tZolv[W62IHj1cYFvKG[3bHytcIVv\3SqIF6teIVzdWmwYXygTIl{QC22YXfn[YQhX2WnMjCoNUB1dyB3NkegdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCqdX3hckBGgHCrMkmzSkBk\WyuczDhd5Nme3OnZDDhd{Bl\XO\|b3PpZZRqd25iY3;ud5RidnRiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:mLDDL[EA:KDBwMECzPUDPxE1w	NELDdHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe5Nlc3OCd-Mki3PVI4PjB:L3G+
Antiproliferative assay	NFHzZpJOTEFvTVKtNlMy		MoCyO\|IhcHK\|	M1TtcWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyCvZXHzeZJm\CCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUKudXWgZZN{[XluIFnDOVAhRSByLkK2JO69VS5?	MkPhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OUK3OlAoRjJ6N{myO\|YxRC:jPh?=
Antiproliferative assay	MXTISWszQTOW		M3vqUFczKGi{cx?=	NYXYZ4xLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJTUt{OUPUJINmdGy\|IH3lZZN2emWmIHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvQnz1[UBie3OjeTygTWM2OCB;IECuNlkh\|ryPLh?=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd7Mke2NEc,Ojh5OUK3OlA9N2F-
Antiproliferative assay	NYXTVWhMVU1zUx?=		MWW3NkBpenN?	M2fDVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV2xV{Bk\WyuczDt[YF{fXKnZDDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVLseYUh[XO\|YYmsJGlEPTBiPTCwMlMyKM7:TT6=	NHXwWJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe5Nlc3OCd-Mki3PVI4PjB:L3G+
Function assay	Mk\3TGVMOjl\|		M4jKelEhcHJ?	MXfJcohq[mm2aX;uJI9nKGi3bXHuJIZ2dGxibHXu[5RpKFCNTWnUNUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJJV{cW6pIFXGV{ApOjR5IITvJFI2QSC{ZYPp[JVmeyliYYOgd5Vje3S{YYTlJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCrbX31co9ie2G7LDDLbUA:KDBwNEeg{txONg>?	M4X3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUSxNVk{Lz5{OUm0NVE6OzxxYU6=
Function assay	MUDISWszQTN?		MVKxJIhz	MoXVTY5pcWKrdHnvckBw\iCqdX3hckBnfWyuIHzlcod1cCCSS13ZWFEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{B2e2mwZzDFSnMhMDJ2NzD0c{AzPTlicnXzbYR2\XNrIHHzJJN2[nO2cnH0[UBi\nSncjCxJIhzKGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iaX3teY5w[XOjeTygTWM2OCB;IESuPVQh\|ryPLh?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl2MUG5N{c,Ojl7NEGxPVM9N2F-
Function assay	MVrNSGEuVUJvMkOx	MYWwMlEhfG9iMUCgeW0>	NHy5ToM3KGi{cx?=	NVHDbZJ[UW6qaXLpeIlwdiCxZjDX[YUyKGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gR2RMOSCyaH;zdIhwenmuYYTpc44h[XRiVInyJFE2KGG2IECuNUB1dyBzMDD1UUBi\nSncjC2JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	M1HkNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Function assay	MV7ISWszQTOW	M4\lfFAvOSC2bzCxNEB2VQ>?	NXftNFdEPiCqcoO=	MmTKTY5pcWKrdHnvckBw\iCZZXWxJIlvKEiHS{K5N3Qh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iQ1TLNUBxcG:\|cHjvdplt[XSrb36gZZQhXHm{MUWgZZQhOC5zIITvJFExKHWPIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	M1\oXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Function assay	NXm3c49QUEWNMkmzWC=>	M1[xfVAvOSC2bzCxNEB2VQ>?	NYrmSJZVPiCqcoO=	NX7oT2FzUW6qaXLpeIlwdiCxZjDQUGsyKGmwIFjFT\|I6O1RiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gWGNVWCCyaH;zdIhwenmuYYTpc44h[XRiMD6xJJRwKDFyIIXNJIFnfGW{IE[gbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	NXG3SmtYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3PVI4PjBpPkK4O\|kzPzZyPD;hQi=>
Function assay	MVfNSGEuVUJvMkO=	MVKwMlEhfG9iMUCgeW0>	Mk\LOkBpenN?	NF\qRmFKdmirYnn0bY9vKG:oIGDMT\|EhcW5iaIXtZY4hVUSDLV3CMVI{KGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIGTDWHAheGixc4Doc5J6dGG2aX;uJIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB4IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd7Mke2NEc,Ojh5OUK3OlA9N2F-
qHTS assay	MknKWGM{Og>?			NUTBRXVSeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGTDN\|Ih[2WubIO=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NHO2T\|lWNTJiT2O=			MYnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy\|	NIfu[Gw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	MUjBOlc{			M1;HVJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy\|	MmDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NFHveHNFSU:\			NXvpOJFpeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO=	MlrtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NUCyZ41GW2Gxcz2y			MWnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy\|	NYi3R5pMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	NVfm[XpNSlRvM{e=			MnvBdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy\|	NW\DZXFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	M2nLbXJF			M{LGfpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>?	NXLEZXVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	MnfDV2suVi2VSB?=			MlHidWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NUjzfGdFSlRvMUK=			M3:zVpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?=	MoLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	M2fH[G5DOTZ2Mx?=			M2XnOJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO=	NGDhWXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	NGjUbWNQUFNvNUC=			NG\mfldyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	MYXCWE0yOg>?			MVHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDCWE0yOiClZXzsdy=>	M{XmTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	M{PafGRCV1l?			NYnlZ2VneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>?	M4DLO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	NHTocHBUUy2QLWPI			MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNVOKIHPlcIx{	NFG4O3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	MonpVog1OQ>?			NELz[JdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?=	M2XRcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	NHjab29CPjd\|			MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=>	MnPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NWHucXVUVUdiNkOgLFYuXEdiUjm=			M2fVUJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ\|IDi2MXRIKFJrIHPlcIx{	MnTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NWHI[HFZXS1{IF;T			Ml\zdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWU0zKE:VIHPlcIx{	NIHN[G09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	NIr4XW9QUFNvNUC=			M{DMNZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG9JWy13MDDj[Yxtew>?	M1rVO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	NIm4UpdTcDRz			Ml\idWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog1OSClZXzsdy=>	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NUnJUGlwWkR?			M2PCdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJFKGOnbHzz	NX3DR3NWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	M4H3Z3NLNUeETUK=			M1m5UJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz	NUTa[JN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	NWPlXlAzW0tvTj3NRy=>			MnTldWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO=	Mm\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	M{X4fG5DNUWEY{G=			NVfZboxweUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	MkfiUGFPNTV?			MkXldWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy\|	M1y2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	M3zDWXJpOTh?			M4XrT5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	M3XMfXNLNUeETUK=			NGXs[JpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy\|	NV\xcmJKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	MojuV4Fwey1{			M4rjSJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNid3NvMjDj[Yxtew>?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-Cdk1(Y15) / Cdk1; PubMed: 25609063 Clin Cancer Res The impact of a 24 hour pre-treatment of MK-1775 on p-Cdk1 was assessed by western blotting. Because of low basal phosphorylation, GBM22 was irradiated (RT) with 10 Gy. p-KAP1(S824) / p-Chk2(T68) / p-Chk1(S345); PubMed: 25609063 Clin Cancer Res Western blot evaluation of GBM6 and GBM22 short-term explant cultures 24 hours after treatment with either MK-1775 or TMZ or the combination. PARP / CF-PARP / pH3(S10) / p-CDC25c(S216) / p-CDK2(Y15); PubMed: 25458954 Cancer Lett Pancreatic cancer cells were treated with vehicle control or 500 nM MK-1775 for 48 h. Whole cell lysates were subjected to Western blotting and probed with anti-PARP, -p-H3, -γH2AX, -p-CHK1, -CHK1, -p-CDC25C, -p-CDK1, - CDK1, -p-CDK2, -CDK2, or -β-actin antibody. WEE1; PubMed: 27616351 Sci Rep MIA PaCa2, PANC-1, Hs 766T, Capan-1 and PL11 cells were treated with MK-1775 (400 nM/L) and/or MMC (150 nM/L) for 24 hours. Whole cell lysates were prepared using RIPA and western blot was performed to assess the protein expression of WEE1 (90 kDa), pCDK1(y15) (34 kDa), CDK1 (34 kDa) and GAPDH (36 kDa) as loading control.	25609063 25458954 27616351
Immunofluorescence	tubulin / p-HH3(S10); PubMed: 30755439 Clin Cancer Res FaDu and UNC7 cells were treated with adavosertib (500 nM), alisertib (250 nM), or adavosertib + alisertib for 24 hours and followed by immunofluorescent staining with anti-tubulin (Green) and anti-pHH3 (S10; Red). Nucleus was stained with DAPI. (A) Representative images of mitotic cells were captured by confocal microscopy. Scale bar: 10 µm. γH2AX; PubMed: 25609063 Clin Cancer Res A) γH2AX foci formation in GBM6 and GBM22 were assessed 24 h after a single treatment of 300 nM MK-1775. Cleaved caspase-3 / pH3; PubMed: 27616351 Sci Rep MIA PaCa2 cells were treated and dual stained with pH3 to observe mitotic entry; and cleaved caspase 3 (CL-CSP3) to observe caspase 3 activity.	30755439 25609063 27616351
Growth inhibition assay	Cell viability; PubMed: 25458954 Cancer Lett Pancreatic cancer cell lines were cultured in 96-well plates at 37℃ for 48 h in complete medium with variable concentrations of MK-1775 and viable cell numbers were determined using MTT reagent and a microplate reader. The data are presented as means ± standard errors from at least 3 independent experiments. IC50; PubMed: 25084614 J Hematol Oncol AML cell lines were cultured for 72 h in complete medium with variable concentrations of MK-1775 (MK) and viable cell numbers were determined using MTT assays. IC50 values were calculated as drug concentration necessary to inhibit 50% growth compared to untreated control cells.	25458954 25084614

体内研究

MK-1775(~20 mg/kg)单独治疗对WiDr异种移植物表现出最小的抗肿瘤作用，在大鼠体内第3天时T/C为69%。在裸鼠HeLa-luc和TOV21G-shp53异种移植模型中，MK-1775单独的抗肿瘤效能是温和的。^[1]

激酶实验： ^[1]	- 合并体外激酶试验: 使用重组人Wee1。激酶反应使用10 μM ATP，1.0 μCi of [γ-³³P]ATP，和2.5 μg poly(Lys, Tyr)作为底物，在逐渐增加浓度的MK-1775存在下，在30°C环境中进行30分钟。整合到底物的放射性被捕获到MultiScreen-PH平板，在液体闪烁计数器上计数。
细胞实验： ^[1]	- 合并 Cell lines: WiDr，NCI-H1299，TOV21G，和 HeLa细胞 Concentrations: 在DMSO中溶解，终浓度为~10 μM Incubation Time: 24小时 Method: 细胞在gemcitabine存在或不存在下处理24小时，然后用MK-1775再处理24小时。细胞活性使用SpectraMax通过WST-8试剂盒测定。细胞内caspase-3/7活性使用Caspase-3/7 Glo试剂盒测定。 (Only for Reference)
动物实验： ^[1]	- 合并 Animal Models: 负荷WiDr，HeLa-luc，或TOV21G-shp53肿瘤的免疫缺陷裸鼠(F344/NJcl-rnu) Dosages: ~20 mg/kg/day Administration: 口服 (Only for Reference)

参考文献

[1] Hirai H, et al. Mol Cancer Ther, 2009, 8(11), 2992-3000.

溶解度 (25°C)

体外	DMSO	80 mg/mL (159.8 mM)
	Ethanol	10 mg/mL (19.97 mM)
	Water	0.0001 mg/mL (0.0 mM)
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+5% Tween 80+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Adavosertib (MK-1775) SDF

分子量	500.6
化学式	C₂₇H₃₂N₈O₂
CAS号	955365-80-7
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	AZD1775

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03668340	Recruiting	Drug: AZD1775	Uterine Cancer	Dana-Farber Cancer Institute\|AstraZeneca	October 22 2018	Phase 2
NCT03028766	Active not recruiting	Drug: AZD1775\|Drug: Cisplatin\|Radiation: Radiotherapy	Hypopharynx Squamous Cell Carcinoma\|Oral Cavity Squamous Cell Carcinoma\|Larynx Cancer	University of Birmingham\|AstraZeneca\|Cancer Research UK	June 22 2017	Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
How to prepare MK1775 methylcellulose solution? and how to prepare methylcellulose itself? Once make the MK1775 methylcellulose solution, how should i keep it?
回答：
MK1775 in 0.5% methylcellulose is a suspension or emulsion, and it is ok to treat mice orally. It is recommended to dissolve methylcellulose in saline. It will take some time to dissolve methylcellulose, and you can vortex it for a while. The MK1775 methylcellulose solution can be stored at 4°C for a week.

研究领域

产品类型

定制您的化合物库

Adavosertib (MK-1775)

客户使用Selleck生产的Adavosertib (MK-1775)发表文献128篇：

产品安全说明书

Wee1抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Adavosertib (MK-1775) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2020-01-06)

技术支持

常见问题及建议解决方法

Wee1 Signaling Pathway Map

相关Wee1产品