Dihydrotestosterone (DHT)

For research use only. Not for use in humans.

目录号:S4757 别名: androstanolone, stanolone, 5α-DHT 中文名称:双氢睾酮

Dihydrotestosterone (DHT) Chemical Structure

CAS No. 521-18-6

Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT)是一种内源性的雄激素性类固醇和激素,是雄激素受体的激动剂,其亲和力比睾酮高2-3倍,诱导雄激素受体信号的效力比睾酮高3倍。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 810.81 现货
RMB 1286.81 现货
RMB 3030.86 现货
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产品安全说明书

Androgen Receptor抑制剂选择性比较

生物活性

产品描述 Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT)是一种内源性的雄激素性类固醇和激素,是雄激素受体的激动剂,其亲和力比睾酮高2-3倍,诱导雄激素受体信号的效力比睾酮高3倍。
靶点
Androgen Receptor [1]
()
体外研究

在雄激素受体阳性的膀胱癌UMUC3和TCC-SUP细胞中,dihydrotestosterone (DHT)可在mRNA和蛋白水平上增加EGFR和ERBB2的表达。在AR阳性细胞中,DHT还可上调EGFR磷酸化水平及其下游蛋白AKT(pAKT)和ERK1/2(pERK)的磷酸化水平[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CV-1 cell Moj4SpVv[3Srb36gZZN{[Xl? Ml\ERYdwdmm|dHnjJIFkfGm4aYT5JEhGSzVyKTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDWk0yKGOnbHysJGVEPTB;MD6wNFYh|ryP M4q3[|k5PzF3M{S=
MDA-MB-453 cell MVXGeY5kfGmxbjDhd5NigQ>? M3PMV2Rqe3CuYXPlcYVvfCCxZjDbN2heTEiWIH\yc40hcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE2GQT3NRk01PTNiY3XscJMh[nlid3jvcIUh[2WubDDy[YNmeHSxcjDibY5lcW6pIHHzd4F6NCCNaU2wMlAxODJizszN NFjCclcyPzB|NEGxOy=>
HepG2 cell NUnpXYhLTnWwY4Tpc44h[XO|YYm= MYDBZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[WO2aY\heIlwdixiSVO1NF0xNjBzOEKg{txO NEPSdJMyPzF2OUi2Oi=>
MDA453 cell MYjGeY5kfGmxbjDhd5NigQ>? MWjEbZNxdGGlZX3lcpQhd2ZiW{PIYWRJXCCocn;tJIh2dWGwIFHSJIlvKE2GQUS1N{Bk\WyuczygT4k:OC5yMEOg{txO NYPXSVRrOTdzOEGxOFE>
mouse C2C12 cell M1;6fWZ2dmO2aX;uJIF{e2G7 NV7PfYJJSWexbnnzeEBi[3Srdnn0fUBifCC{YYSgRXIhfHKjboPm[YN1\WRiaX6gcY92e2ViQ{LDNVIh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTB;MD6wNlgh|ryP NXj0UWVrOTdzOEGxOFE>
monkey COS7 cell NE[3S|hHfW6ldHnvckBie3OjeR?= M4XaNGJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKG2xbnvlfUBEV1N5IHPlcIx{KGK7IIfoc4xmKGOnbHygZolv\GmwZzDhd5NigSxiS3m9NE4xODB{IN88US=> MmDrNVg1PDJ7MUK=
CHO cell NVHNfWhoTnWwY4Tpc44h[XO|YYm= MYXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGTHVlUh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDifUBtfWOrZnXyZZNmKGG|c3H5MEBGSzVyPUSuOFkh|ryP NYDDXo1EOTh|MEeyPVQ>
COS7 cell NX3LTmVHTnWwY4Tpc44h[XO|YYm= NX3FdFNvSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBidmS{b3flckBz\WOncITvdkBYPzRzQzDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[XO|ZYPz[YQh[XNibIXjbYZmemG|ZTDhZ5Rqfmm2eTDh[pRmeiB{NDDodpMh[nlicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCxNkDPxE1? M{TiTFIzODl2Mke5
COS7 cell MYrGeY5kfGmxbjDhd5NigQ>? M4LxOGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4KgWFg4P0FibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUWg{txO MkfJNlIxQTR{N{m=
COS7 cell NWjiNIxJTnWwY4Tpc44h[XO|YYm= M37xSmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEORU{egZ4VtdHNiYYPz[ZN{\WRiYYOgcJVkcW[ncnHz[UBi[3Srdnn0fUBi\nSncjCyOEBpenNiYomgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxPjJizszN M3rkN|IzODl2Mke5
LNCaP-hr cell NF\KfHFHfW6ldHnvckBie3OjeR?= M33kd|Mh\GG7cx?= NVHKbo5nSWexbnnzeEBi[3Srdnn0fUBifCCDbnTyc4dmdiC{ZXPldJRweiCWOEe3RUBufXSjboSgbY4hcHWvYX6gUG5E[VBvaIKgZ4VtdHNiYYPz[ZN{\WRiYYOgdJJwe3SjdHWgd5Bm[2moaXOgZY51cWenbjDz[YNz\XSrb36gcYVie3W{ZXSgZYZ1\XJiMzDkZZl{KGK7IHXufplu\S2rbX31co9ie3OjeTygSWM2OD1yLkCxOUDPxE1? M2q0N|I{OTl7NEe3
LNCaP-hr cell NGP3Z5FHfW6ldHnvckBie3OjeR?= M3zRflMh\GG7cx?= MnLvRYdwdmm|dDDhZ5Rqfmm2eTDheEB4cWymIIT5dIUhSW6mcn;n[Y4hemWlZYD0c5IhcW5iaIXtZY4hVE6FYWCtbJIh[2WubIOgZZN{\XO|ZXSgZZMheHKxc4TheIUhe3CnY3nmbYMh[W62aXflckB{\WO{ZYTpc44hdWWjc4Xy[YQh[W[2ZYKgN{Bl[Xm|IHL5JIVvgnmvZT3pcY12dm:jc4PhfUwhTUN3ME2wMlAxPjJizszN M2HTTVI{OTl7NEe3
HEK293 cell M3jQdGZ2dmO2aX;uJIF{e2G7 M1PiN2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5zODDuUS=> NXTRNZdHOjV|MEW2PFg>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / Cyclin D2 / Cyclin D3 / Cyclin E2 / Cyclin A1 / AR; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h), and immunoblot analysis of cell lysates reveals that cyclin D1 and D2 protein expression decreased substantially with DHT treatment compared to vehicle.

p-CDK2 / CDK2 / CDK4 / CDK6 / CDKN1A / p-RB / RB ; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h). Immunoblot analysis of cell lysates reveals that CDK4 and CDK6 protein expression decreased and CDKN1A expression increased with DHT treatment compared to vehicle. CDK2 phosphorylation at threonine 160 (T160) decreased nearly 50% with androgen treatment at 24 and 48 hours, albeit not significantly, and total CDK2 protein expression remained unchanged. Relative to total RB expression, the levels of various phosphorylated forms of RB (S780 and S807/811) decreased over time with androgen treatment. 

p27 / p-p27 ; 

PubMed: 22139837     


CWR22Rv1 or CWR22R3 cells were stimulated with 10 nm DHT for 6 h, with the addition of MG132 during the last 2 h before harvesting. Proteins were then immunoblotted.

AR / PP1 ; 

PubMed: 26636645     


HeLa cells were transfected with Flag-PP1α and HA-AR and then incubated for overnight in androgen-depleted medium without or with androgen (10 nM of DHT) as indicated for blotting. 

26372468 22139837 26636645
Immunofluorescence
FOXO1 / p27 ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with DHT (10 nm) for 4 h. The cells were immunostained for FOXO1 and p27 and examined with a confocal microscope. 

AKT(S473) / PKCα(S657) ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with 10 nm DHT (−/+ DHT) for 4 h. The cells were immunostained for AKT or PKCα phosphorylation and examined with a confocal microscope. Bars: 20 μm

22139837
体内研究 DHT可减轻SOD1-G93A小鼠的肌萎缩症状并增加其体重。DHT的处理增加了肌肉中胰岛素样生长因子1的表达,通过逆向轴浆运输发挥其肌肉营养性和神经营养性效果。其处理还减缓了神经肌肉接头处的去神经支配作用、以及减少轴突和运动神经元的丢失。DHT处理的SOD1-G93A小鼠改善了运动行为,生命期限也有所延长[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: 膀胱癌细胞
  • Concentrations: 1 nM
  • Incubation Time: 24 h
  • Method:

    对铺于24孔板的、达到50%-60%融合率的细胞进行MMTV-luc报告质粒和PRL-TK-luc质粒共转染实验。转染完6小时后,将培养基替换为含活性炭处理胎牛血清的培养基,加入或不加入配体(DHT、HF或同时加入),处理24小时。收集细胞、溶解、进行荧光素酶活性检测。


    (Only for Reference)
动物实验:

[2]

- 合并
  • Animal Models: SOD1-G93A mutant mice with the congenic C57BL/6J background
  • Dosages: 5 mg
  • Administration: silastic implant
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 58 mg/mL (199.69 mM)
Ethanol 58 mg/mL (199.69 mM)
Water Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 290.44
化学式

C19H30O2

CAS号 521-18-6
储存条件 粉状
溶于溶剂
别名 androstanolone, stanolone, 5α-DHT

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04032067 Not yet recruiting Drug: GV1001 placebo|Drug: Proscar placebo Benign Prostatic Hyperplasia (BPH) GemVax & Kael August 28 2019 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID