Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献48篇:

客户使用该产品的5个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells MW\GeY5kfGmxbjDBd5NigQ>? M4frOVExKM7:TR?= MV6yJIg> MkG2SG1UVw>? NFqyRnJKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ M2rCV|k3PDd4NUS=
N1E-115 NXvZOHduTnWwY4Tpc44hSXO|YYm= M1OwWVExKM7:TR?= M3zYNVIhcA>? M332TWROW09? MYfJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz MkLjPVY1PzZ3NB?=
HeLa M36xXWZ2dmO2aX;uJGF{e2G7 NGHYNnQyOCEQvF2= M2PsNVMxKG2rbh?= NXXQfVB[UW6qaXLpeJMhfGinIH\vdo1ifGmxbjDv[kB{fHKnc4Og[olj\XK|IHHu[EB1cGViYYPz[Y1jdHlib3[geolv[3WuaX6tZ49vfGGrbnnu[{Bnd2OjbDDh[Ihme2mxboO= NYXSfGNnQTZ4OEC3Ni=>
CCL39 MVTGeY5kfGmxbjDBd5NigQ>? MUOzNEDPxE1? MYKzNEBucW5? NFfFdWREd22ybHX0[Yx6KGGkb3zpd4hmeyCjY4TpeoF1cW:wIH;mJG5iNUhiZYjjbIFv\2W{IF7ISVEh[nliaX70[YdzcW6| NFy1fZY6Pjl|M{iy
Mesothelial cells from rat mesentery MXHJcpZie2m4ZTDBd5NigQ>? NFLrPFA{OCEQvF2= NGnvbJIzOCCq NUHRc3VqSmyxY3vzJIlvfmG|aY\lJIFkfGm4aYT5 NYfQUGh1QTl|MEi3Ni=>
NIH3T3 NWfqe2YxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEX6VIgyOCEQvF2= NYrQdWN7OThiZB?= MUXEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NFiwUooyODB{MUO4Oi=>
Dbl-d MojBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\Id3oyOCEQvF2= MYCxPEBl M1THZ3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NIn4W3IyODB{MUO4Oi=>
Dbl-e NWrHfm81T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYKxNEDPxE1? NUnQOXc5OThiZB?= NUG1UmtRVW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= M33NUVExODJzM{i2
mNET1-d NYDPV3RnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXhS4syOCEQvF2= MWWxPEBl NEnjeIpUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1PuXVExODJzM{i2
mNET1-e NUnoW3JUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVexNEDPxE1? MXixPEBl NFT5VWhUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NG\vb4oyODB{MUO4Oi=>
Ras-2 M3iwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV2xNEDPxE1? NG\vTIMyQCCm NYTiRpVIW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NFHrNncyODB{MUO4Oi=>
Ras-4 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHhbFk2OTBizszN NFXjXWIyQCCm NFK2UmRUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NXTVOmNJOTByMkGzPFY>
Src-1 MnfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PB[FExKM7:TR?= NYDuPXhtOThiZB?= NFTybohFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MnyxNVAxOjF|OE[=
Src-4 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHQNVAh|ryP Mlj4NVgh\A>? M2DUeWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NV\VW|VlOTByMkGzPFY>
NIH3T3 MlLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVqxNEDPxE1? NITNN2UyQCCm NGnBbW5Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp M1G5SlExODJzM{i2
Src-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV2xNEDPxE1? MlHNNVgh\A>? NYrSVI1pTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MX[xNFAzOTN6Nh?=
Src-2 M4\kOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYCxNEDPxE1? MoXvNVgh\A>? NFrCU29Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NV;PPG01OTByMkGzPFY>
SW620 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3v4UlExKM7:TR?= MWKxPEBl NIrRUI5Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NYe1cZgzOTByMkGzPFY>
HCT15 NYK1NndHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEL3XWcyOCEQvF2= MUOxPEBl MkTXSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> M17UOlExODJzM{i2
HCT116 M3nX[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWqxNEDPxE1? MVWxPEBl MnjBV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> MX6xNFAzOTN6Nh?=
LS174T NFTtb5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXqxNEDPxE1? MVGxPEBl M3nDXG1w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? NF\yOpIyODB{MUO4Oi=>
Neonatal rat ventricular myocytes MYPGeY5kfGmxbjDBd5NigQ>? MUWxNEDPxE1? MXG0PEBp MVHJcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> MmLtNVA{QDZ4MUO=
Stellate Cell M3PsemZ2dmO2aX;uJGF{e2G7 M2fMXFI2KM7:TR?= NF23S|UyPSCvaX6= MXnJcohq[mm2czDmc5Ju[XSrb36gc4YhTi2jY4TpckB{fHKnc4Og[olj\XK|IHHu[EBxcG:|cHjvdplt[XSrb36gc4YhdXmxc3nuJIxq\2i2IHPoZYlv M1jZcFExPjByNEm2
Rat Vascular Smooth Muscle Cells NIrjUm9HfW6ldHnvckBCe3OjeR?= NUfmRnV7OTBizszN NVWxRplKOiCq M{DSRWlvcGmkaYTzJIFv\2mxdHXud4lvKEmLLXnu[JVk\WRiaInw[ZJ1em:yaIm= NUG1UoZ1OTB4NEKzNVc>
PC3 NWP0Om44TnWwY4Tpc44hSXO|YYm= NUHhPYdxOjVizszN NFfsO5MyKGh? NHHDTFlKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? MYWxNFczODR5MR?=
PC3 M2rIPG1q\3KjdHnvckBCe3OjeR?= MUSyOUDPxE1? MVqxJIg> MVvJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= Mk\GNVA4OjB2N{G=
PC3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUi4Z|dLOjVizszN MnjCNVchcA>? M{LJVGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M2PwelExPzJyNEex
LNCaP Mn\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33HV|I2KM7:TR?= M{HZdVE4KGh? NEfYcGhFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MYqxNFczODR5MR?=
Rat hepatic stellate cells NGPUeIFHfW6ldHnvckBCe3OjeR?= Mo\ZN|Ah|ryP M1jYflQ5KGh? NXzyO25kTGmvaX7pd4hmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzczJuIHHu[EBl\WO{ZXHz[ZMhdmW5IFTORUB{gW62aHXzbZM> MmLYNVA5PDV4NkO=
Pancreatic acinar cells Ml3aSpVv[3Srb36gRZN{[Xl? NFO5c2gyOMLizszN MknzO|AhdWmw M2HkdHBwfGWwdHnheIV{KEOFSz3zeIlufWyjdHXkJJBidmO{ZXH0bYMh\W68eX3lJJNm[3KndHnvci=> MlrFNVI4PDVyOEC=
C2C12 NF;ncYVHfW6ldHnvckBCe3OjeR?= MlnqNVDDqM7:TR?= M1\T[FYhcA>? Mk\RVJJmfmWwdIOgeIhmKHOncnnu[UBxcG:|cHjvdplt[XSrb36gc4YhUVKVLUGgbY5lfWOnZDDifUBqdnO3bHnuJIFv\C:xcjDUUmYu|rF? MXWxOlI3PzF{NB?=
PC 12 NWPBNWJGTnWwY4Tpc44hSXO|YYm= NF\ZVVEyOMLizszN MXqyOEBp Ml3IRZR1\W63YYTld{Bk[XSnY3jvcIFucW6nIHLpc5N6dnSqZYPpdy=> NFzQUIIyPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells MlKxR5l1d3SxeHnjJGF{e2G7 NFvjVWwzOCEEtV2= NVPTXW8yOjRiaB?= M4f6VXBzd22xdHXzJIN6TVNiY3XscEB{fXK4aY\hcC=> NVrXPYV3OTh7NEC4OVU>
TSGH 8301 M3zyUm1q\3KjdHnvckBCe3OjeR?= MXOyNEDDvU1? NVzQeoltOSCq NEKzOIVKdmO{ZXHz[ZMh[2WubDDtbYdz[XSrb36= NX7IdFV4OTl6OU[0O|U>
Swiss3T3 MnzOR49td267LX\vdo1qdmdiQYPzZZk> MmfoNVAhyrWP MXqxN{Bl MnezTY5kemWjc3XzJJBzd3O2YYTlJINmdGxiY3;sc456NW[xcn3pcoch[WO2aY\peJk> MYmyNVQ3PDlyMh?=
HT22 NIPDd3pEgXSxdH;4bYMhSXO|YYm= Mme5NVAhyrWP MlvsNVMhcA>? Mnf0VJJwfGWldIOgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDu[ZVzd26jbDDk[YF1cA>? MVmyNlgyODh|NR?=
Salivary gland stem cells NYXhXGNOTnWwY4Tpc44hSXO|YYm= MX2xNEDDvU1? MXy3JIQ> MnjXVoVlfWOnczDTS3NEKHOnbnXzZ4Vv[2V? M{fiTVI2QDB2NU[w

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用:
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID