Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

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RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献50篇:

客户使用该产品的6个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells M2DxRWZ2dmO2aX;uJGF{e2G7 M1;0ZVExKM7:TR?= M1LVWlIhcA>? MmXpSG1UVw>? NVvyeVlCUW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? NHm4[Hg6PjR5NkW0
N1E-115 M1y3eWZ2dmO2aX;uJGF{e2G7 NIjZWHIyOCEQvF2= M{PYS|IhcA>? NVTT[VZ6TE2VTx?= NYD4[4Z7UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? M2HJVlk3PDd4NUS=
HeLa MmjlSpVv[3Srb36gRZN{[Xl? M1G1elExKM7:TR?= MYKzNEBucW5? NEK5WXJKdmirYnn0d{B1cGViZn;ycYF1cW:wIH;mJJN1emW|czDmbYJmenNiYX7kJJRp\SCjc4PlcYJtgSCxZjD2bY5kfWyrbj3jc451[WmwaX7nJIZw[2GuIHHkbIV{cW:wcx?= NXT1[ppUQTZ4OEC3Ni=>
CCL39 NGfSdFBHfW6ldHnvckBCe3OjeR?= MlnVN|Ah|ryP MojCN|AhdWmw MVjDc41xdGW2ZXz5JIFjd2yrc3jld{Bi[3SrdnH0bY9vKG:oIF7hMWgh\XilaHHu[4VzKE6KRUGgZpkhcW62ZXfybY5{ NHvoXW46Pjl|M{iy
Mesothelial cells from rat mesentery MXfJcpZie2m4ZTDBd5NigQ>? MUezNEDPxE1? MmWxNlAhcA>? NEDucohDdG:la4OgbY53[XOrdnWgZYN1cX[rdIm= M2LJU|k6OzB6N{K=
NIH3T3 NXPC[mt4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWO4NpJCOTBizszN MnL5NVgh\A>? Mlf3SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NVrlbGJuOTByMkGzPFY>
Dbl-d MkPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUCxNEDPxE1? M3Pie|E5KGR? M{i5UHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M2j6UVExODJzM{i2
Dbl-e MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUixNEDPxE1? MYSxPEBl MX\Nc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NV\XRVMzOTByMkGzPFY>
mNET1-d MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\LNVAh|ryP NULUfplSOThiZB?= NGfi[|FUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NFfnNHoyODB{MUO4Oi=>
mNET1-e MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTWcVEyOCEQvF2= NIrndHYyQCCm M4TsVHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NHr4dXYyODB{MUO4Oi=>
Ras-2 NX\XVG93T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrjeJBJOTBizszN M3f1dlE5KGR? M{P1W3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NWjZ[|FuOTByMkGzPFY>
Ras-4 NHHSfJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NET0OlYyOCEQvF2= NETLRmoyQCCm MYrTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> M4P2XVExODJzM{i2
Src-1 NULhZ3AxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{T6XVExKM7:TR?= Mof1NVgh\A>? M1T3XGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NXL5[4RwOTByMkGzPFY>
Src-4 NGj6PIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfVT5oyOCEQvF2= NEXSN40yQCCm M4\yO2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NGrqPHYyODB{MUO4Oi=>
NIH3T3 Mn3HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NED2[YEyOCEQvF2= M1LQOlE5KGR? NVzGTmdPTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MXixNFAzOTN6Nh?=
Src-1 NVfn[IhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWWxVIh3OTBizszN NVrXV5Q1OThiZB?= NUXUdmNMTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MY[xNFAzOTN6Nh?=
Src-2 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fYVVExKM7:TR?= MX6xPEBl MnmxSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MWCxNFAzOTN6Nh?=
SW620 NVfBfWVZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnQUWt2OTBizszN Mk[4NVgh\A>? NYS2SWJSTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MUmxNFAzOTN6Nh?=
HCT15 NV[ySm1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvUXIU3OTBizszN M332VFE5KGR? NXjPV5FHTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M2XNflExODJzM{i2
HCT116 NX7QR2drT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXyxNEDPxE1? MUexPEBl NHfOWIFUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MmTGNVAxOjF|OE[=
LS174T NV[zV4kxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DI[|ExKM7:TR?= Mn;JNVgh\A>? MWDNc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NYrZVm1EOTByMkGzPFY>
Neonatal rat ventricular myocytes NGXFbGxHfW6ldHnvckBCe3OjeR?= MVWxNEDPxE1? Mnz3OFghcA>? M4DLPGlvcGmkaYTzJGVVNTFvaX7keYNm\CCrbnPy[YF{\XNiaX6gdJJwfGWrbjDzfY51cGW|aYOsJINmdGxic3n6[UBidmRibYnv[oljemmubHHyJI9z\2GwaYrheIlwdg>? Ml;qNVA{QDZ4MUO=
Stellate Cell MnzKSpVv[3Srb36gRZN{[Xl? NVLxTZR2OjVizszN M2fKRVE2KG2rbh?= NGfLSlRKdmirYnn0d{Bnd3KvYYTpc44hd2ZiRj3hZ5RqdiC|dILld5Mh\mmkZYLzJIFv\CCyaH;zdIhwenmuYYTpc44hd2ZibYnvd4lvKGyrZ3j0JINp[Wmw NEDTc|YyODZyMES5Oi=>
Rat Vascular Smooth Muscle Cells NIL2SItHfW6ldHnvckBCe3OjeR?= MojmNVAh|ryP MXyyJIg> MnzRTY5pcWKrdIOgZY5ocW:2ZX7zbY4hUUlvaX7keYNm\CCqeYDldpRzd3CqeR?= M174dVExPjR{M{G3
PC3 M3;rOWZ2dmO2aX;uJGF{e2G7 MkDMNlUh|ryP MXmxJIg> NVr1PVQ4UW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? M1LmO|ExPzJyNEex
PC3 MWTNbYdz[XSrb36gRZN{[Xl? NVHjUJFkOjVizszN NHP2RY8yKGh? MWLJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= NFnnXXEyODd{MES3NS=>
PC3 M2W4PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLWNlUh|ryP M3TWd|E4KGh? MnPhSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NEDvVmsyODd{MES3NS=>
LNCaP NFrNe5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWmzbXB3OjVizszN M3vvfVE4KGh? NWfXOZVPTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M2O2NFExPzJyNEex
Rat hepatic stellate cells MoLFSpVv[3Srb36gRZN{[Xl? MmHrN|Ah|ryP NWOxcGNjPDhiaB?= NHLzVpVFcW2rbnnzbIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSZJsOixiYX7kJIRm[3KnYYPld{Bv\XdiRF7BJJN6dnSqZYPpdy=> MkHINVA5PDV4NkO=
Pancreatic acinar cells NEjKeXBHfW6ldHnvckBCe3OjeR?= NV;wb|ZSOTEEoN88US=> NVv2d2lSPzBibXnu NWjsO|RvWG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v M1nZRlEzPzR3MEiw
C2C12 M4\mXmZ2dmO2aX;uJGF{e2G7 NX\oNYFLOTEEoN88US=> MYO2JIg> M1OyUnBz\X[nboTzJJRp\SC|ZYLpcoUheGixc4Doc5J6dGG2aX;uJI9nKEmUUz2xJIlv\HWlZXSgZpkhcW6|dXzpckBidmRxb4KgWG5HNc7z M{nj[FE3OjZ5MUK0
PC 12 Mn6wSpVv[3Srb36gRZN{[Xl? NWHUTm05OTEEoN88US=> NX7QTZVXOjRiaB?= NYTRRYlWSXS2ZX71ZZRmeyClYYTlZ4hwdGGvaX7lJIJqd3O7boTo[ZNqew>? NGLyPZQyPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells MlHyR5l1d3SxeHnjJGF{e2G7 M2DjdlIxKML3TR?= MmTJNlQhcA>? NH;NTo9Rem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> NHP4dVEyQDl2MEi1OS=>
TSGH 8301 NH3BZlVOcWe{YYTpc44hSXO|YYm= NIS5fnMzOCEEtV2= MkDLNUBp NEfPVpJKdmO{ZXHz[ZMh[2WubDDtbYdz[XSrb36= MXyxPVg6PjR5NR?=
Swiss3T3 MkTmR49td267LX\vdo1qdmdiQYPzZZk> MYmxNEDDvU1? NYnue2t4OTNiZB?= NHnLWXFKdmO{ZXHz[ZMheHKxc4TheIUh[2WubDDjc4xwdnlvZn;ycYlv\yCjY4Tpeol1gQ>? MVuyNVQ3PDlyMh?=
HT22 MVPDfZRwfG:6aXOgRZN{[Xl? NGfpV3IyOCEEtV2= M4X0PFE{KGh? NFTRU5BRem:2ZXP0d{Bi\2GrboP0JIdtfXSjbXH0[U1qdmS3Y3XkJI5mfXKxbnHsJIRm[XSq MkPZNlI5OTB6M{W=
Salivary gland stem cells MlfYSpVv[3Srb36gRZN{[Xl? NX74VnhQOTBiwsXN Mly2O{Bl NU\2WWhpWmWmdXPld{BUT1OFIIPlcoV{[2WwY3W= NGjFO2YzPThyNEW2NC=>

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID