Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献46篇:

客户使用该产品的5个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells MoS4SpVv[3Srb36gRZN{[Xl? MkXmNVAh|ryP M4DWbFIhcA>? NYDZNow5TE2VTx?= MWDJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz MmjhPVY1PzZ3NB?=
N1E-115 NVnTOWFMTnWwY4Tpc44hSXO|YYm= NWjIZ5d{OTBizszN M4PLd|IhcA>? M1j3UGROW09? NGfBeHNKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ MmrEPVY1PzZ3NB?=
HeLa MnXPSpVv[3Srb36gRZN{[Xl? M{TYWVExKM7:TR?= NV\Dd|FyOzBibXnu NIfGToFKdmirYnn0d{B1cGViZn;ycYF1cW:wIH;mJJN1emW|czDmbYJmenNiYX7kJJRp\SCjc4PlcYJtgSCxZjD2bY5kfWyrbj3jc451[WmwaX7nJIZw[2GuIHHkbIV{cW:wcx?= NH\yWpE6PjZ6MEey
CCL39 NX72bY1nTnWwY4Tpc44hSXO|YYm= MVOzNEDPxE1? M33jTVMxKG2rbh?= NHK1eoFEd22ybHX0[Yx6KGGkb3zpd4hmeyCjY4TpeoF1cW:wIH;mJG5iNUhiZYjjbIFv\2W{IF7ISVEh[nliaX70[YdzcW6| NWLkfIpVQTZ7M{O4Ni=>
Mesothelial cells from rat mesentery Mlv5TY53[XOrdnWgRZN{[Xl? MnTYN|Ah|ryP NFHGdVEzOCCq MmLnRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 NILXT3g6QTNyOEey
NIH3T3 NEDadGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DNcVExKM7:TR?= MnSzNVgh\A>? Mn7DSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MnjvNVAxOjF|OE[=
Dbl-d NFrKPWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYGxNEDPxE1? NGnWPGUyQCCm MXjTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NHvkXGkyODB{MUO4Oi=>
Dbl-e MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf6fGQyOCEQvF2= MWGxPEBl NXfpcI1wVW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= M2Xj[FExODJzM{i2
mNET1-d Mm\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUmxNEDPxE1? NYW4PGVXOThiZB?= MX7TeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NGjreJkyODB{MUO4Oi=>
mNET1-e NInlSIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{H2ZVExKM7:TR?= MUWxPEBl MULTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NYrtUop6OTByMkGzPFY>
Ras-2 M2nQPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DlVlExKM7:TR?= NVXvSmY2OThiZB?= MnXnV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NGjUO2cyODB{MUO4Oi=>
Ras-4 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXKxNEDPxE1? MVexPEBl NYfGV2k{W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NYK3OFRjOTByMkGzPFY>
Src-1 NFTnelNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moj3NVAh|ryP M{\MbVE5KGR? MX\Ec4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To Mn7kNVAxOjF|OE[=
Src-4 NILOdWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHoTI0yOCEQvF2= MVuxPEBl M3jDTmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NETNTIUyODB{MUO4Oi=>
NIH3T3 MkPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3frcVExKM7:TR?= MWSxPEBl MmLtSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MYOxNFAzOTN6Nh?=
Src-1 MlzCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLZO24yOCEQvF2= MkLJNVgh\A>? MWjEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MVSxNFAzOTN6Nh?=
Src-2 MkLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUGxNEDPxE1? NXvt[mRxOThiZB?= NF7qNZZFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NX\ONHFyOTByMkGzPFY>
SW620 NH\NTWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;1NVAh|ryP MUKxPEBl M1TWS2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NEOxZY0yODB{MUO4Oi=>
HCT15 NHf3WopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVixNEDPxE1? NEjNeGkyQCCm NYWzbnFUTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MX2xNFAzOTN6Nh?=
HCT116 NXzSSpd4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVKxNEDPxE1? M3W2O|E5KGR? MV7TeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MnnNNVAxOjF|OE[=
LS174T NGXFdnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3r6WFExKM7:TR?= MVKxPEBl NFfvdYNOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq M2XpZlExODJzM{i2
Neonatal rat ventricular myocytes MWPGeY5kfGmxbjDBd5NigQ>? NEXqdooyOCEQvF2= NVzvbm1oPDhiaB?= NWfSVVQ6UW6qaXLpeJMhTVRvMT3pcoR2[2WmIHnuZ5Jm[XOnczDpckBxem:2ZXnuJJN6dnSqZYPpd{wh[2WubDDzbZpmKGGwZDDtfY9ncWK{aXzsZZIhd3KpYX7pfoF1cW:w NHnvUYcyODN6Nk[xNy=>
Stellate Cell NGfSdJpHfW6ldHnvckBCe3OjeR?= NX3P[GRUOjVizszN MlPSNVUhdWmw NYO4Um5QUW6qaXLpeJMh\m:{bXH0bY9vKG:oIF[tZYN1cW5ic4Ty[ZN{KG[rYnXyd{BidmRicHjvd5Bpd3K7bHH0bY9vKG:oIH35c5NqdiCuaXfoeEBkcGGrbh?= NEPz[HEyODZyMES5Oi=>
Rat Vascular Smooth Muscle Cells M2iybWZ2dmO2aX;uJGF{e2G7 MUGxNEDPxE1? M2DZelIhcA>? M3TmS2lvcGmkaYTzJIFv\2mxdHXud4lvKEmLLXnu[JVk\WRiaInw[ZJ1em:yaIm= NVXmTndUOTB4NEKzNVc>
PC3 NX\U[YYxTnWwY4Tpc44hSXO|YYm= MV[yOUDPxE1? MWCxJIg> NFjicZZKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? MnLmNVA4OjB2N{G=
PC3 NH7YZVdOcWe{YYTpc44hSXO|YYm= MXqyOUDPxE1? Ml7PNUBp NIDNcW5KdmirYnn0d{B1cGViQl3GRk1EVSCjbnSgeIhmKEWJRj3zeIlufWyjdHXkJI1q\3KjdHnvci=> NG\VNVcyODd{MES3NS=>
PC3 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXFNlUh|ryP M3\MUlE4KGh? NHf0WHBFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NEfNc3QyODd{MES3NS=>
LNCaP M2fvVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TTZlI2KM7:TR?= MoSxNVchcA>? NUDIR4tmTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MVKxNFczODR5MR?=
Rat hepatic stellate cells MU\GeY5kfGmxbjDBd5NigQ>? MkT4N|Ah|ryP MnPQOFghcA>? MWLEbY1qdmm|aHXzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiRYLrNkwh[W6mIHTlZ5Jm[XOnczDu[ZchTE6DIIP5cpRp\XOrcx?= NVHsWmtROTB6NEW2OlM>
Pancreatic acinar cells M{DQXGZ2dmO2aX;uJGF{e2G7 MX2xNOKh|ryP M1XhdlcxKG2rbh?= MlriVI91\W62aXH0[ZMhS0ONLYP0bY12dGG2ZXSgdIFv[3KnYYTpZ{Bmdnq7bXWgd4VkemW2aX;u NHWxNI0yOjd2NUC4NC=>
C2C12 NGiyRVhHfW6ldHnvckBCe3OjeR?= M4PsUVExyqEQvF2= M1LifFYhcA>? MnvGVJJmfmWwdIOgeIhmKHOncnnu[UBxcG:|cHjvdplt[XSrb36gc4YhUVKVLUGgbY5lfWOnZDDifUBqdnO3bHnuJIFv\C:xcjDUUmYu|rF? M3nuO|E3OjZ5MUK0
PC 12 M1XifmZ2dmO2aX;uJGF{e2G7 MVWxNOKh|ryP NF3IWWkzPCCq Mne1RZR1\W63YYTld{Bk[XSnY3jvcIFucW6nIHLpc5N6dnSqZYPpdy=> MYWxOlIyQTR{NB?=
Cynomolgus monkey embryonic stem cells MYXDfZRwfG:6aXOgRZN{[Xl? NHr3eJQzOCEEtV2= MmrhNlQhcA>? M{DOXnBzd22xdHXzJIN6TVNiY3XscEB{fXK4aY\hcC=> M{jDNVE5QTRyOEW1
TSGH 8301 MVTNbYdz[XSrb36gRZN{[Xl? M2i4cVIxKML3TR?= NGLPSYMyKGh? MUPJcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? M3rrNlE6QDl4NEe1
Swiss3T3 M3rmVmNwdG:weT3mc5JucW6pIFHzd4F6 MYexNEDDvU1? NXzaSoE6OTNiZB?= MnTRTY5kemWjc3XzJJBzd3O2YYTlJINmdGxiY3;sc456NW[xcn3pcoch[WO2aY\peJk> NHTmVHgzOTR4NEmwNi=>
HT22 MkLjR5l1d3SxeHnjJGF{e2G7 M3z2XFExKML3TR?= NHvhW3gyOyCq M3PISHBzd3SnY4TzJIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgcoV2em:wYXyg[IVifGh? Mn7UNlI5OTB6M{W=
Salivary gland stem cells MV7GeY5kfGmxbjDBd5NigQ>? M{LWSVExKML3TR?= Mn\xO{Bl MnjFVoVlfWOnczDTS3NEKHOnbnXzZ4Vv[2V? Mn\mNlU5ODR3NkC=

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次加入纯溶剂:
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

Related Antibodies

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID