Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献48篇:

客户使用该产品的5个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells NIC2TZVHfW6ldHnvckBCe3OjeR?= M1fIWFExKM7:TR?= MnvVNkBp NVvpeG1OTE2VTx?= M3iyXWlvcGmkaYTzJJRp\SCjc4PlcYJtgSCxZjDtbYNzd3S3YoXs[ZMh[W6mIHnueIVzdWWmaXH0[UBncWyjbXXueJMhfG9iZn;ycUBmgHSnbnTl[EBxem:lZYPz[ZM> MlPRPVY1PzZ3NB?=
N1E-115 NWm3bYtJTnWwY4Tpc44hSXO|YYm= MlPFNVAh|ryP M{fvXVIhcA>? MUjEUXNQ MY\Jcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz NX3LNFBDQTZ2N{[1OC=>
HeLa MWLGeY5kfGmxbjDBd5NigQ>? NVTVPYVXOTBizszN Mnj5N|AhdWmw NFzYbXdKdmirYnn0d{B1cGViZn;ycYF1cW:wIH;mJJN1emW|czDmbYJmenNiYX7kJJRp\SCjc4PlcYJtgSCxZjD2bY5kfWyrbj3jc451[WmwaX7nJIZw[2GuIHHkbIV{cW:wcx?= MY[5OlY5ODd{
CCL39 MnHCSpVv[3Srb36gRZN{[Xl? MUSzNEDPxE1? NF\DfYo{OCCvaX6= M3nJR2NwdXCuZYTlcJkh[WKxbHnzbIV{KGGldHn2ZZRqd25ib3[gUoEuUCCneHPoZY5o\XJiTljFNUBjgSCrboTl[5JqdnN? M{HlSlk3QTN|OEK=
Mesothelial cells from rat mesentery NIHtZ2JKdn[jc3n2[UBCe3OjeR?= MmDYN|Ah|ryP NEfTZ|kzOCCq NVnPfXc1SmyxY3vzJIlvfmG|aY\lJIFkfGm4aYT5 MYC5PVMxQDd{
NIH3T3 Mke4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUCxNEDPxE1? MkXiNVgh\A>? M1m0bGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NU\6ellwOTByMkGzPFY>
Dbl-d NWL3bINvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2qxb|ExKM7:TR?= MV[xPEBl NInWNJNUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? Mni1NVAxOjF|OE[=
Dbl-e M1K4cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjRdpdDOTBizszN NYHUS5E2OThiZB?= NVfwc25LVW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= MW[xNFAzOTN6Nh?=
mNET1-d NGTiSpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGW0UYcyOCEQvF2= NVrzT2c{OThiZB?= NVSxOYpDW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? MkWyNVAxOjF|OE[=
mNET1-e MlPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\6fHJ2OTBizszN NH;z[IsyQCCm NVKxPVg2W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? M4fzd|ExODJzM{i2
Ras-2 NYXaN5hNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXqxNEDPxE1? M{izSVE5KGR? NYC2OnBxW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? M4T4TVExODJzM{i2
Ras-4 M2qyfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\kOVY2OTBizszN NGewfHgyQCCm MXzTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> M3\KZlExODJzM{i2
Src-1 MlLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\UOFRmOTBizszN MV2xPEBl M1zUWGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MVuxNFAzOTN6Nh?=
Src-4 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7ofooyOCEQvF2= MWWxPEBl M{nIeGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M3\UTFExODJzM{i2
NIH3T3 NV;lWmN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHSVWNJOTBizszN MVyxPEBl NHPsO|RFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MlKwNVAxOjF|OE[=
Src-1 M33tdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4riNlExKM7:TR?= M1fWUVE5KGR? NGTTeVdFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp M2fJdlExODJzM{i2
Src-2 MoC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVuxNEDPxE1? M16zO|E5KGR? M1nQ[WRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NIjqNZAyODB{MUO4Oi=>
SW620 MmiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTUe4wyOCEQvF2= M2HlVVE5KGR? NVnXUoJqTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M1zJPFExODJzM{i2
HCT15 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TrbVExKM7:TR?= MmjXNVgh\A>? M2f3UmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MkTZNVAxOjF|OE[=
HCT116 NE\CTVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvjPVJuOTBizszN MUixPEBl MoThV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NYXTbFR3OTByMkGzPFY>
LS174T MnTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXGxNEDPxE1? M13yV|E5KGR? NFz3cnVOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NHq2TFcyODB{MUO4Oi=>
Neonatal rat ventricular myocytes M{fSU2Z2dmO2aX;uJGF{e2G7 NFPySHkyOCEQvF2= MlflOFghcA>? MlPpTY5pcWKrdIOgSXQuOS2rbnT1Z4VlKGmwY4LlZZNmeyCrbjDwdo91\WmwIIP5cpRp\XOrczygZ4VtdCC|aYrlJIFv\CCveX;mbYJzcWyuYYKgc5Jo[W6renH0bY9v MYqxNFM5PjZzMx?=
Stellate Cell MUHGeY5kfGmxbjDBd5NigQ>? NYXhRng{OjVizszN Mn;SNVUhdWmw M1LSO2lvcGmkaYTzJIZwem2jdHnvckBw\iCILXHjeIlvKHO2cnXzd{BncWKncoOgZY5lKHCqb4PwbI9zgWyjdHnvckBw\iCveX;zbY4hdGmpaISgZ4hicW5? NEjofGkyODZyMES5Oi=>
Rat Vascular Smooth Muscle Cells MkfiSpVv[3Srb36gRZN{[Xl? NInGcFkyOCEQvF2= MXeyJIg> MV7Jcohq[mm2czDhcodqd3SnboPpckBKUS2rbnT1Z4VlKGi7cHXyeJJweGi7 NGHpNIIyODZ2MkOxOy=>
PC3 MYjGeY5kfGmxbjDBd5NigQ>? Moq1NlUh|ryP NGLXXJYyKGh? M2Paemlv\HWlZYOgcY9zeGixbH;nbYNidCClaHHu[4V{ M2HlTlExPzJyNEex
PC3 NWDnV5d3VWmpcnH0bY9vKEG|c3H5 M3XWNVI2KM7:TR?= NWjIdJN{OSCq MYjJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= M3zHTVExPzJyNEex
PC3 NXfuOXpbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGSySm0zPSEQvF2= NVHtOIl5OTdiaB?= MnHSSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> M4jRS|ExPzJyNEex
LNCaP NXP5dpdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX72Opl5OjVizszN M{nTPVE4KGh? M1j3OWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NHnPTlIyODd{MES3NS=>
Rat hepatic stellate cells NIfzU45HfW6ldHnvckBCe3OjeR?= M3X0VFMxKM7:TR?= MVm0PEBp NYLDTpdVTGmvaX7pd4hmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzczJuIHHu[EBl\WO{ZXHz[ZMhdmW5IFTORUB{gW62aHXzbZM> Ml3qNVA5PDV4NkO=
Pancreatic acinar cells Ml\LSpVv[3Srb36gRZN{[Xl? NX7QPIdEOTEEoN88US=> NE\vSW44OCCvaX6= NV[1NoY3WG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v MmrDNVI4PDVyOEC=
C2C12 NETVd21HfW6ldHnvckBCe3OjeR?= NHjoeoIyOMLizszN MonOOkBp MWDQdoV3\W62czD0bIUhe2W{aX7lJJBpd3OyaH;yfYxifGmxbjDv[kBKWlNvMTDpcoR2[2WmIHL5JIlve3WuaX6gZY5lN2:{IGTOSk3PuQ>? MXSxOlI3PzF{NB?=
PC 12 Mo\ISpVv[3Srb36gRZN{[Xl? M2DV[lExyqEQvF2= NEjJbnMzPCCq MWPBeJRmdnWjdHXzJINifGWlaH;sZY1qdmViYnnvd5lvfGinc3nz NGnXNngyPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells NXPITYd5S3m2b4TvfIlkKEG|c3H5 M{j4OlIxKML3TR?= NUS1e41lOjRiaB?= NFjpOFRRem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> MWKxPFk1ODh3NR?=
TSGH 8301 NUH6SZNHVWmpcnH0bY9vKEG|c3H5 M3LDdVIxKML3TR?= MWGxJIg> NVzBT5F{UW6lcnXhd4V{KGOnbHygcYloemG2aX;u MYGxPVg6PjR5NR?=
Swiss3T3 M2TGbGNwdG:weT3mc5JucW6pIFHzd4F6 MXuxNEDDvU1? NVrwfHZ1OTNiZB?= MojsTY5kemWjc3XzJJBzd3O2YYTlJINmdGxiY3;sc456NW[xcn3pcoch[WO2aY\peJk> M{\kZVIyPDZ2OUCy
HT22 Mn3lR5l1d3SxeHnjJGF{e2G7 NF7TW5MyOCEEtV2= Ml;2NVMhcA>? NIfqV4FRem:2ZXP0d{Bi\2GrboP0JIdtfXSjbXH0[U1qdmS3Y3XkJI5mfXKxbnHsJIRm[XSq MWmyNlgyODh|NR?=
Salivary gland stem cells M{X5XGZ2dmO2aX;uJGF{e2G7 NY\GUpg3OTBiwsXN M{n2SFch\A>? MWXS[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? M3Lre|I2QDB2NU[w

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用:
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID