CHIR-99021 (CT99021) HCl

目录号:S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 (CT99021) HCl是CHIR-99021的盐酸盐, 是GSK-3α/β抑制剂,IC50为10 nM/6.7 nM。 其对GSK-3的选择性比对Cdc2和ERK2高500倍以上。

规格 价格 库存 购买数量  
RMB 2780.99 现货
RMB 733.53 现货
RMB 1373.92 现货
RMB 4650.09 现货
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客户购买Selleck的此次产品后发表的文献70篇:

客户使用该产品的9个实验数据:

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

产品安全说明书

GSK-3抑制剂选择性比较

生物活性

产品描述 CHIR-99021 (CT99021) HCl是CHIR-99021的盐酸盐, 是GSK-3α/β抑制剂,IC50为10 nM/6.7 nM。 其对GSK-3的选择性比对Cdc2和ERK2高500倍以上。
靶点
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
体外研究

CHIR-99021作用于GSK-3比作用于其最接近的同系物CDC2和ERK2,及其他蛋白激酶选择性高500倍。此外,CHIR-99021只与一组22种药理相关的受体微弱结合,且对一组23种非激酶的酶几乎没有抑制活性。CHIR-99021作用于表达胰岛素受体的CHO-IR细胞,诱导糖原合成酶(GS)激活,EC50为0.763 μM。[1]CHIR-99021(3 μM)作用于3T3-L1前脂肪细胞,除了模拟胰岛素的作用,抑制GSK-3,使胞内游离β-catenin增加1.9倍,模仿经典Wnt信号通路。在分化的前3天,CHIR-99021处理,抑制脂肪细胞分化,IC50为0.3 μM,通过抑制CCAAT/增强子结合蛋白α(C/EBPα)和过氧化物酶体增殖物激活受体γ(PPARγ)的诱导作用。[2] 不像LiCl和AR-A014418,CHIR-99021处理,即使在高浓度,不会降低INS-1E细胞活力。相反,CHIR-99021增加INS-1E细胞增殖,这种作用具有剂量依赖性,且显著抑制高糖和高棕榈酸诱导的INS-E细胞死亡,这种作用具有浓度依赖性。CHIR-99021浓度低至1 μM,处理离体大鼠胰岛,促进β细胞复制,5 μM CHIR-99021处理,使细胞复制增长2-3倍。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 Ml;ISpVv[3Srb36gRZN{[Xl? MUOzJO69VQ>? M{jQR|QhcA>? NI\pRZdFVVOR NWjBN21YUW6qaXLpeJMheHKnYXTpdI9kgXSnIHTp[oZmemWwdHnheIlwdg>? M3;6c|EzODV3MkCw
insulin receptor–expressing CHO-IR MmG4SpVv[3Srb36gRZN{[Xl? MXG1NFAhdk1? Mn7GN|AhdWmw NV\ifJF5TE2VTx?= MUnBZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>? NWr5PYRrOTJ4ME[0PVc>
H4IIE MUXGeY5kfGmxbjDBd5NigQ>? Ml35NVAh|ryP MoT3NlQhcA>? M1ftU2ROW09? MlK1VoVofWyjdHXzJO6zNWOjdHXubY4h[WO2aY\peJkh[W6mIGTJVmUh[2:wdHHpcolv\yCycn;tc5RmeiCjY4Tpeol1gQ>? MXKxOVM2ODF7NR?=
Oct4/Sox2/Klf4-infected MEFs MkjPSpVv[3Srb36gRZN{[Xl? MknENVAh|ryP MnSxNlgh\A>? M4PZN2ROW09? M4HpcXBzd22xdHXzJG1GTnNicnXwdo9oemGvbXnu[{BVemGwc3T1Z4VlKGK7IF;jeFQtKFOxeEKsJIFv\CCNbH[0 M2rvZlE6QDN7MEW1
Sca-1+ HPC Mn;1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnG2NkDPxE1? NWnscnYxPiCm NF;wOmdFVVOR M1nSbHN6dmW{Z3nzeIlk[WyueTDwdo9ud3SnczDTZ4EuOSwEoFjQR{Bxem:uaX\ldoF1cW:wIHHu[EBkd2yxbomg[o9zdWG2aX;uJJRw\2W2aHXyJJdqfGhiRVfG NUPUcYdHOjF4MkSzOFg>
HMC1.2 NI[5VYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlq2N|Ah|ryP NYDnbpplPzJiaB?= Mmf1SG1UVw>? MnjoVoVlfWOnczDj[YxtKHO3co\peoFt NV73e3RWOjJyM{mzNFE>
Endothelial cell(EC) M{XkV2Z2dmO2aX;uJGF{e2G7 M2PNc|Mh|ryP M{\k[2ROW09? NIPJVJJRem:vb4Tld{BmdmSxdHjlcIlidCClZXzsJEhGSyliZHnm[oVz\W62aXH0bY9v MnrhNlIxPTJyME[=
PANC1 MVTBdI9xfG:|aYOgRZN{[Xl? MmCxxsA2KM7:TR?= MofaO|IhcA>? MnvCSG1UVw>? NFPLU5hVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? MnHTNlIzODFzOE[=
INS-1E MkD0SpVv[3Srb36gRZN{[Xl? M2DBXFIvPSEQvF2= MVeyOEBp MlPCSG1UVw>? MULJcoR2[2W|IHGgdoVlfWO2aX;uJI9nKE6SMTDveoVz\XiycnXzd4lwdg>? M2jvTlIzPDJ5N{C0
hiPSC-NPs MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDCN-KBkc7:TR?= MWG3NkBp MmPRSG1UVw>? NXrVXJpwUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi MWOyNlY1OjZ6Nx?=
platelets MXnGeY5kfGmxbjDBd5NigQ>? NELOd|IyOCEQvF2= NEHBe4IyPSCvaX6= MnPtSG1UVw>? NUHjNY1JUW6lcnXhd4V{KHSqcn;tZolvNW2nZHnheIVlKHCuYYTlcIV1KGGpZ4Ll[4F1cW:wIHHu[EBCXFBic3XjdoV1cW:w M4HNU|I{OjN7OEe3
DN3 M{nLOGZ2dmO2aX;uJGF{e2G7 M1XvZVExKM7:TR?= NYWyRlgxQTZiaB?= NIT6UG1FVVOR NH;5SWpKdmO{ZXHz[ZMh[2WubDDk[ZZmdG:ybXXueEBidmRicILvcIln\XKjdHnvci=> NFnveXozOzV{Nkm4PS=>
embryonic stem cells (ESC) NH;hcHRHfW6ldHnvckBCe3OjeR?= M2HWWlMhyrWP NIXiUIQyOSCm NGH4XYhFVVOR NVHMe3NOWHKxbX;0[ZMhe2WuZj3y[Y5mf2GuIH;mJI5iy6:4ZT30fZBmKG2xdYPlJIVu[nK7b37pZ{B{fGWvIHPlcIx{KCiHU1Op NV3Je5pmOjN6NEOzNVI>
H460 NXjDTmVUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn65OUDDvU1? NYjROJVGOjRiaB?= M{HxVGROW09? MX7Qdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36= MnG2NlQ{Pjd4OEi=
H157 M{OwPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTmdlM2KML3TR?= Ml;rNlQhcA>? MWPEUXNQ NVvNO|B{WHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v M3fFTFI1OzZ5Nki4
H460 M2eybGFxd3C2b4Ppd{BCe3OjeR?= MVq1JOK2VQ>? NFW3RYQzPCCq MYnEUXNQ M4LsVGF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= M4\6SlI1OzZ5Nki4
H157 M3;wSWFxd3C2b4Ppd{BCe3OjeR?= MY[1JOK2VQ>? MX[yOEBp MX7EUXNQ MWjEc4V{KG6xdDDzbYdvcW[rY3HueIx6KGGoZnXjeEBieG:ydH;zbZM> NH:1emMzPDN4N{[4PC=>
A549 NHHBN2RCeG:ydH;zbZMhSXO|YYm= NUHNc3hEPSEEtV2= NHHm[WkzPCCq MkHsSG1UVw>? Mn7tTY5kemWjc3XzJINmdGxiYYDvdJRwe2m| NXnMbWx1OjR|Nke2PFg>
Neural progenitor cells MUXGeY5kfGmxbjDBd5NigQ>? M13LU|Mh|ryP NILY[o4yOCCm MYfEUXNQ NEnWWlNKdmS3Y3XzJIdmdmW{YYTpc44hd2ZibnX1doFtKHC{b3flcol1d3JiY3XscJM> NF3aWVkzPDZ|OECzOC=>
ES-D3 NYP6PFNHS3m2b4TvfIlkKEG|c3H5 NIDUcG8yOMLizszN NGn6RVE{KGR? MX;EUXNQ NEPtVJpKdmS3Y3XzJI1w\GW{YYTlJIN6fG:2b4jpZ4l1gSC5aYToJGlEPTBib3[gOE46KM7:TR?= M37xTFI1Pzd7M{[1
ES-CCE M33YOmN6fG:2b4jpZ{BCe3OjeR?= NGP5RZUyOMLizszN NGDC[oE{KGR? MVvEUXNQ M1rnXWlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6 MoTiNlQ4Pzl|NkW=
ES-D3 MUPGeY5kfGmxbjDBd5NigQ>? NWH5VmhZPcLizszN M1fCZlMh\A>? NF;nRZZFVVOR NXjNflgzW3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 NGW2RpgzPDd5OUO2OS=>
ES-CCE M4jQN2Z2dmO2aX;uJGF{e2G7 NVPjXWVXPcLizszN MXmzJIQ> MVfEUXNQ MkHMV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 MnP3NlQ4Pzl|NkW=
SW982 NUHJfYh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vub2lEPTB;MD6yNVQyPiEQvF2= NEDpWpVUSU6JRWK=
GCT MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPNemxKSzVyPUCuN|A3PzJizszN MmTQV2FPT0WU
769-P NYHBN2FOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\tUGlEPTB;MD61PFQ6QSEQvF2= MWHTRW5ITVJ?
LOXIMVI MoftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjJUol4UUN3ME2wMlU5QDN{IN88US=> M1XmTXNCVkeHUh?=
D-336MG MnLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFu5cGtKSzVyPUCuPFQ3OiEQvF2= M{nze3NCVkeHUh?=
S-117 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjaOHNzUUN3ME2wMlkzOTNzIN88US=> NI[3OHZUSU6JRWK=
D-247MG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTCTWM2OD1yLkm0PFUzKM7:TR?= M2[4dXNCVkeHUh?=
TYK-nu MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrMPJVKSzVyPUGuNFc6OTJizszN M1jscnNCVkeHUh?=
A498 Mk\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1uxVmlEPTB;MT6zNFQ3QSEQvF2= MXvTRW5ITVJ?
BCPAP M3rxSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[zTWM2OD1zLk[2PFk5KM7:TR?= MV;TRW5ITVJ?
KYSE-180 MkXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zPUGlEPTB;MT63Nlc5QCEQvF2= NIHXc29USU6JRWK=
MIA-PaCa-2 M{iweGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTFwN{W5O|ch|ryP NHvkbYRUSU6JRWK=
HCC1395 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DK[2lEPTB;MT63PFI4OyEQvF2= MVLTRW5ITVJ?
RS4-11 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTFwOUW0NVUh|ryP MonqV2FPT0WU
NCI-H2122 NVrn[ll{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnSVGJKSzVyPUKuNlc3QDRizszN M{nkNXNCVkeHUh?=
SNU-423 NEDuOpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVntTZN4UUN3ME2yMlQ6Pzh5IN88US=> MVHTRW5ITVJ?
SK-LMS-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnm4TWM2OD1{Lk[0NVI5KM7:TR?= NYHGV40xW0GQR1XS
RPMI-7951 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2qzWmlEPTB;Mj63NFE6OyEQvF2= M4flb3NCVkeHUh?=
COLO-829 NWPlWZg1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTJwN{[1OEDPxE1? M1uxZ3NCVkeHUh?=
SCC-9 M4jydGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fDUWlEPTB;Mj65OVQ5PSEQvF2= MlTNV2FPT0WU
A704 M3jzVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfuc2dIUUN3ME2zMlA5OTJizszN NIf6Z2lUSU6JRWK=
HOP-62 NGrNNGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTNwM{[wOVQh|ryP MWHTRW5ITVJ?
HCC1569 NWrJRmRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHTTVlxUUN3ME2zMlQ5OzB3IN88US=> NYPoWpZJW0GQR1XS
A172 M3S1OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\IZ4s5UUN3ME2zMlc2PDl6IN88US=> MUfTRW5ITVJ?
MOLT-16 NX7jbW51T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTNwOEi1OVYh|ryP MVzTRW5ITVJ?
TE-15 M2HjSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v2VmlEPTB;Mz65OVM1OyEQvF2= MXzTRW5ITVJ?
OE19 NHr4c3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zZT2lEPTB;Mz65OlQ2OSEQvF2= M4mzeHNCVkeHUh?=
MHH-ES-1 MmqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITPTHdKSzVyPUSuNFQ2PDVizszN M2XuVHNCVkeHUh?=
NKM-1 NXvNR2VzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzmTWM2OD12LkGzPVcyKM7:TR?= M33INXNCVkeHUh?=
RCC10RGB Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7y[odKSzVyPUSuNlk1PzlizszN NUfSRnp2W0GQR1XS
BxPC-3 NW\Gc2FLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTDZ4NKSzVyPUSuO|A{QTFizszN NWPLWGJQW0GQR1XS
ALL-PO NXrV[oViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLLTWM2OD12LkizPFU5KM7:TR?= MkfoV2FPT0WU
SK-OV-3 Ml;nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTRwOUmzOEDPxE1? NGGwb2JUSU6JRWK=
SW1710 NFLE[3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXYZ2E6UUN3ME21MlQzPDV4IN88US=> NXToeZd6W0GQR1XS
NCI-H2452 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrXeY5KSzVyPUWuOFU3OjhizszN NWXBS3lzW0GQR1XS
D-392MG NV;NZpNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUO5RW9bUUN3ME21MlQ6OTR3IN88US=> M2nMVnNCVkeHUh?=
LB2518-MEL Mlf1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonCTWM2OD13LkeyOlI4KM7:TR?= M4XRXnNCVkeHUh?=
BL-41 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHlVXM4UUN3ME21MlgyOjZ4IN88US=> NIX6V3lUSU6JRWK=
WM-115 MnvBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rEZWlEPTB;NT64NVUxQSEQvF2= MWHTRW5ITVJ?
SK-MEL-2 NEm4V4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDzTWM2OD14LkO4N|Q1KM7:TR?= MV7TRW5ITVJ?
CGTH-W-1 NHHzU2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;ETWM2OD14LkSxO{DPxE1? MUfTRW5ITVJ?
BB65-RCC Mk\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zKUWlEPTB;Nj61N|E6PSEQvF2= Mm[zV2FPT0WU
CAL-85-1 MmexS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWGyT3c6UUN3ME22MlYxQTN4IN88US=> NIH5e3BUSU6JRWK=
RT-112 NF3yfZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTdwMU[0OVYh|ryP MmLsV2FPT0WU
MC116 NY\XWIhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGO1WWJKSzVyPUeuNlg3QTFizszN MlPXV2FPT0WU
G-361 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDNfVVvUUN3ME23MlQ3PTZzIN88US=> MVXTRW5ITVJ?
SW1417 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTdwNkS1PFQh|ryP NWHYS2ZNW0GQR1XS
MV-4-11 NVvZTFVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjLcpNnUUN3ME24MlU1OzB2IN88US=> M2nQNXNCVkeHUh?=
CAL-72 M{npSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXXR5RkUUN3ME24Mlc3OTF|IN88US=> NVHVfVlyW0GQR1XS
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HAL-01 NH\UcIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXZNmVKSzVyPUGyNk41QTlizszN NVfJ[JdTW0GQR1XS
HT-29 Mn\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTF{Mj63O|gh|ryP MofwV2FPT0WU
OPM-2 NWDtdVN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfXPHZKSzVyPUGyN{4yPzZizszN M{j0cnNCVkeHUh?=
SW1463 NUHQfG91T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TKUWlEPTB;MUK0MlA4PyEQvF2= MVHTRW5ITVJ?
NCI-H2087 NEXiblRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\yTWM2OD1zMkSuNlg{KM7:TR?= Mor3V2FPT0WU
UMC-11 NYPUXpBsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fjR2lEPTB;MUK2MlA{QCEQvF2= M17SXHNCVkeHUh?=
EB-3 M1uzUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\EfWlEPTB;MUK3MlAyOyEQvF2= MlHjV2FPT0WU
HEC-1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HxTmlEPTB;MUK5MlE6QCEQvF2= MUDTRW5ITVJ?
LU-99A NIDnRXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TTTmlEPTB;MUK5Mlk{OSEQvF2= MnHvV2FPT0WU
VMRC-RCZ MnfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTF|MD6zO|Uh|ryP NWn0WlI5W0GQR1XS
MFH-ino MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;ETWM2OD1zM{GuNVE1KM7:TR?= MX\TRW5ITVJ?
HT-1080 NILxfIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\pTWM2OD1zM{KuNlA1KM7:TR?= MmnNV2FPT0WU
CTB-1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHizWWdKSzVyPUGzNk45QTdizszN M1y0eXNCVkeHUh?=
Capan-2 M3HpNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm[0TWM2OD1zM{OuPVc2KM7:TR?= NEPZbmJUSU6JRWK=
BB30-HNC Mn;SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTF|ND6xNVYh|ryP NE[5NpVUSU6JRWK=
CP50-MEL-B NHTCeoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnLTWM2OD1zM{SuNkDPxE1? NWPMcWN7W0GQR1XS
JEG-3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTF|ND6zOVkh|ryP NFrkT5NUSU6JRWK=
NCI-H1437 M1m0OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXFO3ZKSzVyPUGzOE42QSEQvF2= NYLhZnFuW0GQR1XS
NOS-1 NXrpPG96T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkW1TWM2OD1zM{SuO|Y2KM7:TR?= NFLaeplUSU6JRWK=
G-402 MlvOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPEcFVlUUN3ME2xN|UvOjl3IN88US=> MUTTRW5ITVJ?
OAW-42 NGHmUVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnrUHVKSzVyPUGzO{45PTZizszN MV3TRW5ITVJ?
Ca-Ski NGTaSnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjHTWM2OD1zM{iuPFMyKM7:TR?= M{DmeXNCVkeHUh?=
BHT-101 M1;CRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTF2MD6yNFgh|ryP MUnTRW5ITVJ?
OVCAR-4 NWK3TnJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTF2MD63OFkh|ryP NX3kc4h3W0GQR1XS
Detroit562 NEm1UXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LIZmlEPTB;MUSxMlQ3PiEQvF2= MkWwV2FPT0WU
OCI-AML2 NGTIZnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTF2MT61NFch|ryP MX;TRW5ITVJ?
OE33 M3PhSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWOycmJZUUN3ME2xOFEvPzl6IN88US=> NGDa[YdUSU6JRWK=
YKG-1 NIK1[|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1L2OmlEPTB;MUSyMlg5QSEQvF2= NVrRbYlTW0GQR1XS
CCF-STTG1 NXjxfoNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLxTWM2OD1zNEOuN|M1KM7:TR?= MlXtV2FPT0WU
NCI-H810 NICxT4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTF2Mz62PFch|ryP MlfMV2FPT0WU
SCC-25 NFrmRlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXX0dY5qUUN3ME2xOFQvODB3IN88US=> M3\ITHNCVkeHUh?=
HTC-C3 M3XJSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mkj3TWM2OD1zNEWuNFc2KM7:TR?= NYrxeJpSW0GQR1XS
G-401 Ml\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfQeGtKSzVyPUG0OU45OzVizszN Ml:0V2FPT0WU
OMC-1 MnvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fSdWlEPTB;MUS1Mlk3PyEQvF2= MVXTRW5ITVJ?
LK-2 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPqUFdLUUN3ME2xOFcvOTR|IN88US=> Mm\MV2FPT0WU
SW13 MmmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvsfnJKSzVyPUG0O{4zPTNizszN M1nlcXNCVkeHUh?=
BB49-HNC NV[1RZVUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\j[2lEPTB;MUS3MlQxPCEQvF2= NXflOWt2W0GQR1XS
DMS-114 MoTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTF2Nz60OFEh|ryP M2LMPHNCVkeHUh?=
GCIY NEHOUJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3oU|JmUUN3ME2xOFcvPzl5IN88US=> NWfKO5FkW0GQR1XS
GAMG Moe2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke0TWM2OD1zNEiuNlQ4KM7:TR?= M3HGb3NCVkeHUh?=
KYSE-520 NVXkS2pwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLwTWM2OD1zNEiuOVQh|ryP MnK3V2FPT0WU
LB647-SCLC NHT4XlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\kPGlEPTB;MUS4MlU3OiEQvF2= MljTV2FPT0WU
639-V M37R[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTF2OD63NFUh|ryP NY[xVXRmW0GQR1XS
TE-9 NHr5[pZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTF2OT60OFMh|ryP MVzTRW5ITVJ?
Caov-3 M2GxcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTF2OT63NVQh|ryP NHi5fnFUSU6JRWK=
L-540 NV\ZVJpRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTF2OT63OUDPxE1? MoDMV2FPT0WU
NCI-H748 NIDMXWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\hOYZKSzVyPUG1NE4zODlizszN M33HOnNCVkeHUh?=
GDM-1 NVrBdmd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;RflZKSzVyPUG1NU4yOzVizszN NEi0N3lUSU6JRWK=
GAK NEXIW4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjxTWM2OD1zNUGuN|c1KM7:TR?= NGKxRXRUSU6JRWK=
AsPC-1 NFOyfHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTF3Mj63OlQh|ryP NWfM[W9zW0GQR1XS
MDA-MB-361 M4\KNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;ZUXZKSzVyPUG1N{4yPjRizszN MVvTRW5ITVJ?
TT M2H1Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTF3Mz60PUDPxE1? MYnTRW5ITVJ?
Calu-1 NEDRS5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH25bIRKSzVyPUG1OE4xODNizszN NHPTZ2VUSU6JRWK=
RERF-LC-MS MmjhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHpTWM2OD1zNUSuO|czKM7:TR?= MXfTRW5ITVJ?
DK-MG M3r1c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTF3NT6xPFMh|ryP M3\MbnNCVkeHUh?=
CAL-120 NF;iWnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\jTWM2OD1zNUWuPFIyKM7:TR?= M{nLTnNCVkeHUh?=
HCC1419 MmrxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33E[WlEPTB;MUW2MlA4PyEQvF2= MV;TRW5ITVJ?
DG-75 MmTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTF3Nj62PVQh|ryP NVnEN3hNW0GQR1XS
HCC38 NEH4NmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTF3Nz6zOlkh|ryP MXjTRW5ITVJ?
HCC70 Mm\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTF3OT6yOFIh|ryP NWm0TWhVW0GQR1XS
ES4 M2PzUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTF3OT6zPVYh|ryP M1fyTHNCVkeHUh?=
NCI-H2052 M1TCVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHy4fllKSzVyPUG1PU42QDNizszN NX;wd4RyW0GQR1XS
EFO-27 NYjKWHlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPFTWM2OD1zNkCuOVg1KM7:TR?= MYPTRW5ITVJ?
CAL-62 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;mNXI2UUN3ME2xOlIvPjF|IN88US=> MlLyV2FPT0WU
CHP-212 M{jiPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LXVGlEPTB;MU[zMlYzPiEQvF2= NWXoTVh5W0GQR1XS
AU565 NEW1c2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7JXXBKSzVyPUG2N{43PCEQvF2= M2L6Z3NCVkeHUh?=
Ca9-22 NFPTd2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHSNW1KSzVyPUG2OE4{OjFizszN NHXBZ2hUSU6JRWK=
EFO-21 M{LpNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;kRnRKSzVyPUG2OU41PSEQvF2= M2ryVHNCVkeHUh?=
KINGS-1 NYLMVXRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rHTmlEPTB;MU[3MlM2PCEQvF2= NYLOUFdtW0GQR1XS
JVM-2 NUezU2g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTCfHdWUUN3ME2xOlcvPDVzIN88US=> MmXwV2FPT0WU
OVCAR-5 MnPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{O1R2lEPTB;MU[4MlMxQSEQvF2= MV3TRW5ITVJ?
Calu-3 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrSWm9KUUN3ME2xOlgvOzFzIN88US=> NFyzdWtUSU6JRWK=
CP66-MEL Mk\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfQZYFKSzVyPUG2PE41OTVizszN NWjq[4ZiW0GQR1XS
KYSE-150 NYjpeG5CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPWV2RMUUN3ME2xOlkvPjN4IN88US=> MmXZV2FPT0WU
Daoy M2f3fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3Ob4xKSzVyPUG2PU45QTFizszN MXPTRW5ITVJ?
HUTU-80 M2DRb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDQZ49KSzVyPUG3NU4xOzJizszN NGLBT4tUSU6JRWK=
SW620 M3q4PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIraWlhKSzVyPUG3NU42OzVizszN NYOxRmdYW0GQR1XS
HuO-3N1 NHu2NHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnLTYNKSzVyPUG3Nk4zPzNizszN NFKxXpVUSU6JRWK=
HH M{jIcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjuRpNjUUN3ME2xO|MvQDR6IN88US=> M2XwZXNCVkeHUh?=
ME-180 NEGze5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XYNGlEPTB;MUe0MlAyPiEQvF2= NYXzSHVTW0GQR1XS
SW756 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVK0O3NoUUN3ME2xO|QvQDZ|IN88US=> NInKU5FUSU6JRWK=
LNCaP-Clone-FGC M{fFeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf0TWM2OD1zN{WuPFYyKM7:TR?= M{S5cXNCVkeHUh?=
YT M2PNRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTF5Nj6zPVkh|ryP NYjNT4x[W0GQR1XS
EW-3 M3P6e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmK4TWM2OD1zN{[uOVU2KM7:TR?= MoixV2FPT0WU
COLO-741 MnPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIn5b|FKSzVyPUG3O{42PjFizszN NIe3dFhUSU6JRWK=
SK-NEP-1 NEjZVGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTF5Nz64NFYh|ryP NIq4cVZUSU6JRWK=
NB10 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInrW2tKSzVyPUG3O{45PDZizszN NYDiXlNJW0GQR1XS
SF126 NF\DNGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTF5OT60N|Ih|ryP MmXxV2FPT0WU
NCI-H522 Mnr0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;xbYFKSzVyPUG4NU4xPTlizszN M3r3SHNCVkeHUh?=
PSN1 NWrsNJk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTF6MT62OkDPxE1? NXK0PW1pW0GQR1XS
GI-ME-N MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTF6MT62O|Ih|ryP M16zWnNCVkeHUh?=
SBC-5 NXzieFVTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjNTWM2OD1zOEGuPVM1KM7:TR?= M1jDb3NCVkeHUh?=
ONS-76 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DBeGlEPTB;MUiyMlM5PyEQvF2= NITMXlhUSU6JRWK=
LOUCY MkTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\pcmc1UUN3ME2xPFIvPjh6IN88US=> MmLiV2FPT0WU
SN12C NFfMXZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojPTWM2OD1zOEKuPFI6KM7:TR?= M4rvVHNCVkeHUh?=
A427 NXnURo9pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIqzTW1KSzVyPUG4OE43OzFizszN NYW1R4JlW0GQR1XS
DOK M1;j[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXPTWM2OD1zOESuOlQ{KM7:TR?= MmH3V2FPT0WU
MDA-MB-415 NV3wO2h6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zCTWlEPTB;MUi0MlY2OSEQvF2= NWf5dmhJW0GQR1XS
MRK-nu-1 MnL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTF6ND65O|Ih|ryP MkfDV2FPT0WU
LB2241-RCC MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4O3XmlEPTB;MUi1MlE1PSEQvF2= M33USXNCVkeHUh?=
NCI-H1563 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnpTWM2OD1zOEWuNlA6KM7:TR?= MkLrV2FPT0WU
MDA-MB-453 M2XDTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTF6Nj65OFEh|ryP NETKdJJUSU6JRWK=
MONO-MAC-6 NXXFe21IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIH0eHNKSzVyPUG4O{4{QTJizszN M{fFZ3NCVkeHUh?=
CAPAN-1 NF\ydVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\PfoFKSzVyPUG4O{41PTNizszN MV;TRW5ITVJ?
SK-LU-1 M4HKWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV76b3ZUUUN3ME2xPFgvPDJ6IN88US=> M4jZSXNCVkeHUh?=
NCI-H1299 NVqxcJExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2H6d2lEPTB;MUi4MlcyQSEQvF2= MWXTRW5ITVJ?
KG-1 MkfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTF6OT63PVgh|ryP NUnZZZB6W0GQR1XS
ETK-1 NYftS5g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfoTlVKSzVyPUG5NE4yQDFizszN M3OyN3NCVkeHUh?=
NCI-H838 NX33dm8yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TuXWlEPTB;MUmwMlM1KM7:TR?= M2LnNHNCVkeHUh?=
HCE-4 M3[1NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\NTWM2OD1zOUCuPVkzKM7:TR?= NVjLOVVqW0GQR1XS
A2780 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTF7Mj64PVEh|ryP MkTWV2FPT0WU
COLO-668 NFXhbFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXT2NpFKUUN3ME2xPVMvOTV5IN88US=> M4LqVHNCVkeHUh?=
HGC-27 Moe3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTF7NT61N|gh|ryP MWDTRW5ITVJ?
SW1088 M2rMRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHKzRnVKSzVyPUG5OU43PzdizszN NY\Xd41IW0GQR1XS
KOSC-2 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjUeHFKSzVyPUG5OU45OThizszN NWHDU5o2W0GQR1XS
TE-1 M2HmZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTPVWJKSzVyPUG5Ok4zPiEQvF2= MXjTRW5ITVJ?
SW780 NIm0XIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTF7Nj63N|Qh|ryP MmDVV2FPT0WU
22RV1 NWjIZo9PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE[4PIhKSzVyPUG5O{41OjRizszN NILM[XVUSU6JRWK=
IA-LM MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXOVYpKSzVyPUG5O{45OjJizszN NVfuSIt1W0GQR1XS
TE-5 M2LTWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrofXFHUUN3ME2xPVgvODN|IN88US=> MnXGV2FPT0WU
JVM-3 Mk\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTF7OD60NVkh|ryP MnPZV2FPT0WU
HT55 Ml;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXiSodKSzVyPUG5PU4yQTJizszN NH3p[W1USU6JRWK=
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KARPAS-422 NXzN[IlHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHKWo97UUN3ME23PFkvOzF3IN88US=> MkL2V2FPT0WU
BC-3 Mn;zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzDTWM2OD16MEWuNlA{KM7:TR?= NWnyV|NXW0GQR1XS
NCI-H1155 MkfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfiXFRHUUN3ME24NVgvODV7IN88US=> M{jHN3NCVkeHUh?=
NCI-H1581 NHrPWFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPoTWM2OD17MECuNlMyKM7:TR?= NYDGRpdWW0GQR1XS

... Click to View More Cell Line Experimental Data

体内研究 CHIR-99021按30 mg/kg 剂量口服处理患2型糖尿病的啮齿类动物模型,增强葡萄糖代谢,处理3-4小时后,最大血浆葡萄糖减少近150 mg/dl,而血浆胰岛素保持或低于对照水平。CHIR-99021按16 或 48 mg/kg剂量口服给药ZDF大鼠,1小时后,再口服葡萄糖,显著改善糖耐量,16 mg/kg 和 48 mg/kg处理组的血浆葡萄糖分别减少14%和33%,较高剂量CHIR-99021处理,再口服葡萄糖,还可以降低高血糖。[1]CHIR-99021处理移植小鼠或人造血干细胞(HSC)的受体小鼠,显著增强造血重建功能,说明GSK-3是一种特异性的HSC活性调节剂。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

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无细胞激酶实验:

聚丙烯96孔板中每孔充满了含27 nM GSK-3α 或 29 nM GSK-3β, 和 0.5 μM 生物素-CREB 肽底物的300 μL 缓冲液 (50 mM Tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM 二硫苏糖醇, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) 。各种浓度CHIR-99021加入3.5 μL DMSO中,随后加入50 μL ATP储存液,使所有无细胞实验中ATP终浓度为1 μM。温育后,100 μL等分试样按一式三份转移至每孔含100 μL 50 μM ATP和 20 mM EDTA的Combiplate 8孔板中。1小时后,使用用PBS将孔洗涤5次,填充200 μL闪烁液,密封,放置30分钟后,然后在闪烁计数器中计数。所有步骤在室温下进行。
细胞实验:

[3]

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  • Cell lines: INS-1E
  • Concentrations: 溶于DMSO, 终浓度为20 μM
  • Incubation Time: 1, 或 4 天
  • Method:

    细胞在饥饿培养基(只含5mM葡萄糖,1%胎牛血清)中维持24小时。然后使用不同浓度的CHIR-99021处理细胞的1天或4天。通过使用CyQuant染料进行细胞DNA染色,测量细胞数,当结合DNA就会发出荧光。温育30分钟后,使用FLUOstar Optima酶标仪测量荧光。通过BrdUrd掺入法测定细胞复制。最后4小时,BrdUrd标记溶液加入到培养基中,然后使用FixDenat溶液固定细胞,与单克隆anti-BrdUrd-POD抗体温育。每孔加入底物溶液后,在微孔板发光检测仪上使用Analyst HT检测系统测量光发射。


    (Only for Reference)
动物实验:

[1]

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  • Animal Models: 雌性db/db小鼠或雄性2型糖尿病ZDF大鼠
  • Formulation: 在20mM柠檬酸盐缓冲的15%Captisol中配制成溶液,或在0.5%羧甲基纤维素中配制成悬浮液
  • Dosages: ~48 mg/kg
  • Administration: 口服处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
体内 从左到右依次加入纯溶剂:
5% DMSO+30% PEG 300+ddH2O
24mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 501.8
化学式

C22H18Cl2N8.HCl

CAS号 252917-06-9 (free base)
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • 回答:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • 问题 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • 回答:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • 问题 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • 回答:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID