HCV Protease

信号通路图

研究领域

抑制剂选择性比较

HCV Protease产品

所有产品 HCV Protease抑制剂(19)

产品目录	产品描述	文献引用	实验数据
S1482	Daclatasvir (BMS-790052) Daclatasvir (BMS-790052)是一种高度选择性的HCV NS5A抑制剂，EC50为9-50 pM，在细胞培养中，作用于多种HCV复制基因型和JFH-1基因型2a的感染性病毒。Phase 3。	Cell Rep, 2018, 25(4):833-840 J Steroid Biochem Mol Biol, 2018, 183:142-151 J Chromatogr B Analyt Technol Biomed Life Sci, 2018, 1073:183-190	The toxicity of BMS-790052 (BMS) and Telaprevir (TPV) was measured by seeding 96-well plates to 70% confluence and exposing the cells to up to 50 μM of compound for 72 hours. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was added to each well at a final concentration of 500 μg/ml 4 h before dissolving crystals in 100 μl of DMSO and measuring at 550 nm UV wavelengths. The 50% cytotoxic concentration (CC50) was then calculation using the OD550 value and the following formula: log CC50=log concentration of HPP-[(HPP-50)/(HPP-LPP)×log d HPP: highest protective percentage closest to 50% LPP: Lowest protective percentage closest to 50% d: dilution factor
S1538	Telaprevir (VX-950) Telaprevir (VX-950)是一种丙型肝炎病毒(HCV)NS3-4A丝氨酸蛋白酶抑制剂，IC50为0.354 μM。	Nat Methods, 2018, 15(7):519-522 Biochem Pharmacol, 2018, 155:305-315 Oncotarget, 2017, 8(15):24694-24705	HCV replicon genotype 1b cells were passaged in the presence of G418 alone (medium 694 control) or G418 with miravirsen, SPC4729 (oligonucleotide negative control) or telaprevir for 28 days in fixed concentrations at a multiple (X) of the EC₅₀ of miravirsen or telaprevir. Colony formation was assessed by staining surviving cells with crystal violet.
S1480	Lomibuvir (VX-222, VCH-222) Lomibuvir (VX-222, VCH-222)是一种新型的，有效的，选择性的HCV聚合酶抑制剂，IC50为0.94-1.2 μM，作用于突变型M423T效果低15.3倍，作用于突变型I482L效果低108倍。Phase 2。	J Steroid Biochem Mol Biol, 2018, 183:142-151 Antimicrob Agents Chemother, 2016, 60(12):7060-7066 J Biol Chem, 2016, 291(19):10078-88	Genetic barriers of JTK-853, NNI-A, PF-868554, VX-222, PSI-6130, BMS-790052, and TMC435 for Con1. VX-222 and the other DAAs were treated at 1/3xEC90 for 17 days.
S1183	Danoprevir (ITMN-191) Danoprevir (ITMN-191)是一种肽类抑制剂，作用于丙型肝炎病毒(HCV)的NS3/4A蛋白酶，IC50为0.2-3.5 nM，抑制HCV 1A/1B/4/5/6基因型比抑制2B/3A基因型效果高10倍左右。Phase 2。	Antiviral Res, 2018, 150:79-92 Gastroenterology, 2017, 10.1053/j.gastro.2017.04.021 Antimicrob Agents Chemother, 2016, 60(12):7060-7066	Western blot analysis of HCV NS3 protease cleavage of MAVS. The catalytic efficiency of four proteases (H, H-A156T, 41 and 41-A156T) from individuals H and 41 were tested in the absence or presence of an NS3 protease inhibitor (danoprevir). Expression of the HCV NS3 proteases resulted in cleavage of the lambda cI repressor with MAVS cleavage site. Expression of the protease was induced with IPTG for 3 h. The lambda cI repressor with MAVS cleavage site was not cleaved by an NS3 protease that included a substitution in catalytic residue S139A. Similarly, different catalytic efficiencies were observed with different proteases.
S4935	Asunaprevir Asunaprevir是具有口服活性的HCV NS3抑制剂。HCV NS3是病毒复制蛋白加工所必需的蛋白酶。
S5404	Paritaprevir (ABT-450) Paritaprevir (ABT-450)是NS3/4A抑制剂。
S5403	Ombitasvir (ABT-267) Ombitasvir (ABT-267)是HCV NS5A的抑制剂，具有抗病毒活性。
S5402	Dasabuvir(ABT-333) Dasabuvir(ABT-333) is a non-nucleoside inhibitor of NS5B viral RNA-dependent RNA polymerase that inhibits recombinant NS5B polymerases derived from HCV genotype 1a and 1b clinical isolates with IC50 values between 2.2 and 10.7 nM. It is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases.
S5720	Glecaprevir Glecaprevir is a hepatitis C virus (HCV) nonstructural (NS) protein 3/4A protease inhibitor that targets the the viral RNA replication. It displays IC50 values ranging from 3.5 to 11.3 nM for clinical isolates of HCV genotypes 1a, 1b, 2a, 2b, 3a, 4a, 5a, and 6a.
S5394	Tizoxanide Tizoxanide, a thiazolide anti-infective, is active against anaerobic bacteria, protozoa, and a range of viruses in cell culture models, and is currently in phase II clinical development for treating chronic hepatitis C.
S3728	Grazoprevir Grazoprevir anhydrous is a Hepatitis C Virus NS3/4A Protease inhibitor with IC50 values of 7pM, 4pM, and 62pM for HCV genotype 1a, 1B, and 4 respectively.
S5652	Elbasvir Elbasvir is an NS5A inhibitor, preventing hepatitis C viral RNA replication and virion assembly. Median EC50 values range from 0.2 to 3600 pmol/L, based on genotype.
S7579	Ledipasvir (GS5885) Ledipasvir（GS5885）是一种HCV NS5A polymerase抑制剂，用于治疗丙型肝炎病毒感染。	Sci Rep, 2017, 7(1):9223	(C) Impact of HCV clearance by DAA treatment on the Nrf2 nuclear translocation. HCV infected Huh-7.5 cells day 12 were given two rounds of antiviral treatment with IFN-α, sofosbuvir, ledipasvir or combination of sofosbuvir plus ledipasvir. After 72 hours, cells were split, and then treated again with the same antiviral agent. After two rounds of antiviral treatment, the expressions of HCV core and pNrf2 were measured by immunostaining.
S5062	Daclatasvir Dihydrochloride Daclatasvir是一种具有口服活性的抗病毒剂，抑制HCV的NS5A区域，用于治疗慢性遗传型1和3类的HCV感染。
S3800	Lycorine hydrochloride Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, has been reported to exhibit a wide range of physiological effects, including the potential effect against cancer. It is an HCV inhibitor with strong activity.
S3724	Velpatasvir Velpatasvir是二代NS5A抑制剂，抑制丙型肝炎病毒（HCV）的复制。
S3733	Boceprevir Boceprevir是一种口服的、直接作用于hepatitis C virus (HCV) protease的抑制剂，对NS3蛋白酶的Ki值为14 nM。在治疗慢性肝炎C(genotype 1)中，常与其他抗病毒共同给药。
S5307	PSI-6206 (RO-2433, GS-331007) PSI-6206 (RO-2433, GS-331007)是有效的HCV RNA依赖性RNA聚合酶抑制剂，靶向NS5B聚合酶。
S5015	Simeprevir Simeprevir一种竞争性的、可逆的、非共价结合的大环hepatitis C virus (HCV) NS3/4A protease抑制剂，直接抑制HCV病毒。它对HCV NS3/4A(genotypes 1a, 1b, 2, 4, 5, and 6)的平均IC50值小于13 nM，而对genotype 3的IC50值为37 nM。

产品目录	产品描述	文献引用	实验数据
S1482	Daclatasvir (BMS-790052) Daclatasvir (BMS-790052)是一种高度选择性的HCV NS5A抑制剂，EC50为9-50 pM，在细胞培养中，作用于多种HCV复制基因型和JFH-1基因型2a的感染性病毒。Phase 3。	Cell Rep,2018, 25(4):833-840 J Steroid Biochem Mol Biol,2018, 183:142-151 J Chromatogr B Analyt Technol Biomed Life Sci,2018, 1073:183-190	The toxicity of BMS-790052 (BMS) and Telaprevir (TPV) was measured by seeding 96-well plates to 70% confluence and exposing the cells to up to 50 μM of compound for 72 hours. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was added to each well at a final concentration of 500 μg/ml 4 h before dissolving crystals in 100 μl of DMSO and measuring at 550 nm UV wavelengths. The 50% cytotoxic concentration (CC50) was then calculation using the OD550 value and the following formula: log CC50=log concentration of HPP-[(HPP-50)/(HPP-LPP)×log d HPP: highest protective percentage closest to 50% LPP: Lowest protective percentage closest to 50% d: dilution factor
S1538	Telaprevir (VX-950) Telaprevir (VX-950)是一种丙型肝炎病毒(HCV)NS3-4A丝氨酸蛋白酶抑制剂，IC50为0.354 μM。	Nat Methods,2018, 15(7):519-522 Biochem Pharmacol,2018, 155:305-315 Oncotarget,2017, 8(15):24694-24705	HCV replicon genotype 1b cells were passaged in the presence of G418 alone (medium 694 control) or G418 with miravirsen, SPC4729 (oligonucleotide negative control) or telaprevir for 28 days in fixed concentrations at a multiple (X) of the EC₅₀ of miravirsen or telaprevir. Colony formation was assessed by staining surviving cells with crystal violet.
S1480	Lomibuvir (VX-222, VCH-222) Lomibuvir (VX-222, VCH-222)是一种新型的，有效的，选择性的HCV聚合酶抑制剂，IC50为0.94-1.2 μM，作用于突变型M423T效果低15.3倍，作用于突变型I482L效果低108倍。Phase 2。	J Steroid Biochem Mol Biol,2018, 183:142-151 Antimicrob Agents Chemother,2016, 60(12):7060-7066 J Biol Chem,2016, 291(19):10078-88	Genetic barriers of JTK-853, NNI-A, PF-868554, VX-222, PSI-6130, BMS-790052, and TMC435 for Con1. VX-222 and the other DAAs were treated at 1/3xEC90 for 17 days.
S1183	Danoprevir (ITMN-191) Danoprevir (ITMN-191)是一种肽类抑制剂，作用于丙型肝炎病毒(HCV)的NS3/4A蛋白酶，IC50为0.2-3.5 nM，抑制HCV 1A/1B/4/5/6基因型比抑制2B/3A基因型效果高10倍左右。Phase 2。	Antiviral Res,2018, 150:79-92 Gastroenterology,2017, 10.1053/j.gastro.2017.04.021 Antimicrob Agents Chemother,2016, 60(12):7060-7066	Western blot analysis of HCV NS3 protease cleavage of MAVS. The catalytic efficiency of four proteases (H, H-A156T, 41 and 41-A156T) from individuals H and 41 were tested in the absence or presence of an NS3 protease inhibitor (danoprevir). Expression of the HCV NS3 proteases resulted in cleavage of the lambda cI repressor with MAVS cleavage site. Expression of the protease was induced with IPTG for 3 h. The lambda cI repressor with MAVS cleavage site was not cleaved by an NS3 protease that included a substitution in catalytic residue S139A. Similarly, different catalytic efficiencies were observed with different proteases.
S4935	Asunaprevir Asunaprevir是具有口服活性的HCV NS3抑制剂。HCV NS3是病毒复制蛋白加工所必需的蛋白酶。
S5404	Paritaprevir (ABT-450) Paritaprevir (ABT-450)是NS3/4A抑制剂。
S5403	Ombitasvir (ABT-267) Ombitasvir (ABT-267)是HCV NS5A的抑制剂，具有抗病毒活性。
S5402	Dasabuvir(ABT-333) Dasabuvir(ABT-333) is a non-nucleoside inhibitor of NS5B viral RNA-dependent RNA polymerase that inhibits recombinant NS5B polymerases derived from HCV genotype 1a and 1b clinical isolates with IC50 values between 2.2 and 10.7 nM. It is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases.
S5720	Glecaprevir Glecaprevir is a hepatitis C virus (HCV) nonstructural (NS) protein 3/4A protease inhibitor that targets the the viral RNA replication. It displays IC50 values ranging from 3.5 to 11.3 nM for clinical isolates of HCV genotypes 1a, 1b, 2a, 2b, 3a, 4a, 5a, and 6a.
S5394	Tizoxanide Tizoxanide, a thiazolide anti-infective, is active against anaerobic bacteria, protozoa, and a range of viruses in cell culture models, and is currently in phase II clinical development for treating chronic hepatitis C.
S3728	Grazoprevir Grazoprevir anhydrous is a Hepatitis C Virus NS3/4A Protease inhibitor with IC50 values of 7pM, 4pM, and 62pM for HCV genotype 1a, 1B, and 4 respectively.
S5652	Elbasvir Elbasvir is an NS5A inhibitor, preventing hepatitis C viral RNA replication and virion assembly. Median EC50 values range from 0.2 to 3600 pmol/L, based on genotype.
S7579	Ledipasvir (GS5885) Ledipasvir（GS5885）是一种HCV NS5A polymerase抑制剂，用于治疗丙型肝炎病毒感染。	Sci Rep,2017, 7(1):9223	(C) Impact of HCV clearance by DAA treatment on the Nrf2 nuclear translocation. HCV infected Huh-7.5 cells day 12 were given two rounds of antiviral treatment with IFN-α, sofosbuvir, ledipasvir or combination of sofosbuvir plus ledipasvir. After 72 hours, cells were split, and then treated again with the same antiviral agent. After two rounds of antiviral treatment, the expressions of HCV core and pNrf2 were measured by immunostaining.
S5062	Daclatasvir Dihydrochloride Daclatasvir是一种具有口服活性的抗病毒剂，抑制HCV的NS5A区域，用于治疗慢性遗传型1和3类的HCV感染。
S3800	Lycorine hydrochloride Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, has been reported to exhibit a wide range of physiological effects, including the potential effect against cancer. It is an HCV inhibitor with strong activity.
S3724	Velpatasvir Velpatasvir是二代NS5A抑制剂，抑制丙型肝炎病毒（HCV）的复制。
S3733	Boceprevir Boceprevir是一种口服的、直接作用于hepatitis C virus (HCV) protease的抑制剂，对NS3蛋白酶的Ki值为14 nM。在治疗慢性肝炎C(genotype 1)中，常与其他抗病毒共同给药。
S5307	PSI-6206 (RO-2433, GS-331007) PSI-6206 (RO-2433, GS-331007)是有效的HCV RNA依赖性RNA聚合酶抑制剂，靶向NS5B聚合酶。
S5015	Simeprevir Simeprevir一种竞争性的、可逆的、非共价结合的大环hepatitis C virus (HCV) NS3/4A protease抑制剂，直接抑制HCV病毒。它对HCV NS3/4A(genotypes 1a, 1b, 2, 4, 5, and 6)的平均IC50值小于13 nM，而对genotype 3的IC50值为37 nM。

Tags: HCV protease treatment | HCV protease assay | HCV protease inhibitors review