Hydrocortisone

别名: NSC 10483 中文名称:氢化可的松(皮质醇)

Hydrocortisone是一种由肾上腺产生的类固醇激素或糖皮质激素。

Hydrocortisone Chemical Structure

Hydrocortisone Chemical Structure

CAS: 50-23-7

规格 价格 库存 购买数量
10mM (1mL in DMSO) 1071.75 现货
50mg 811.21 现货
200mg 2211.54 现货
1g 5487.3 现货
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细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
RAW264.7 Antiinflammatory assay 0.5 to 50 ug/ml 4 hrs Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-10 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control 21384845
RAW264.7 Antiinflammatory assay 0.5 to 50 ug/ml 4 hrs Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-6 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control 21384845
RAW264.7 Antiinflammatory assay 0.5 to 50 ug/ml 4 hrs Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL1-beta production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control 21384845
RAW264.7 Antiinflammatory assay 0.5 to 50 ug/ml 4 hrs Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of TNFalpha production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control 21384845
RAW264.7 Antiinflammatory assay 0.5 to 50 ug/ml 4 hrs Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by Griess reaction method relative to control 21384845
RAW264.7 Antiinflammatory assay 10 uM 2 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in TNF-alpha level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control 25515561
RAW264.7 Antiinflammatory assay 10 uM 2 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-6 level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control 25515561
RAW264.7 Antiinflammatory assay 10 uM 2 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-1beta level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control 25515561
RAW264.7 Antiinflammatory assay 10 uM 2 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs relative to vehicle-treated control 25515561
B16F10 Apoptosis assay 20 uM 36 hrs Induction of apoptosis in mouse B16F10 cells assessed as increase in cleaved caspase 3 level at 20 uM after 36 hrs by western blot analysis 26695732
B16F10 Apoptosis assay 20 uM 36 hrs Induction of apoptosis in mouse B16F10 cells assessed as increase in p53 expression at 20 uM after 36 hrs by propidium iodide staining based FACS analysis 26695732
B16F10 Apoptosis assay 20 uM 36 hrs Induction of apoptosis in mouse B16F10 cells assessed as increased Bax expression at 20 uM after 36 hrs by western blot analysis 26695732
B16F10 Apoptosis assay 20 uM 36 hrs Induction of apoptosis in mouse B16F10 cells assessed as increase in cytochrome C level at 20 uM after 36 hrs by western blot analysis 26695732
B16F10 Antitumor activity assay 6.11 mg/kg Antitumor activity against mouse B16F10 cells xenografted in C57BL/J mouse assessed as inhibition of tumor growth at 6.11 mg/kg, ip 26695732
mouse L929 cells Growth inhibition assay 6 days Growth inhibition of mouse L929 cells after 6 days 926113
mouse RAW264.7 cells Function assay 24 h Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction, IC50=0.061 μM 21361338
RAW264.7 Function assay 24 hrs Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry, IC50 = 40.64 μM. 21807513
RAW264.7 Function assay 24 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction, IC50 = 40.64 μM. 23067550
RAW264.7 Antiinflammatory assay 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50 = 43.8 μM. 23755850
L929 Function assay 20 hrs Displacement of 1 x 10'-8 M of [1,2,3-3H]-triamcinolone acetonide from glucocorticoid receptor in soluble fraction of mouse L929 cells after 20 hrs, Kd = 0.043 μM. 926113
RAW264.7 Antiinflammatory assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay, IC50 = 40.64 μM. 20879743
HEK293 Function assay Inhibition of MR-mediated transactivation of galactosidase reporter gene in HEK293 cells expressing 11betaHSD1 16759088
neural precursor cells Function assay Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay 17417631
点击查看更多细胞系数据

生物活性

产品描述 Hydrocortisone是一种由肾上腺产生的类固醇激素或糖皮质激素。
靶点
Glucocorticoid receptor [1]
体外研究(In Vitro)
体外研究活性

在人脑微血管内皮细胞系hCMEC/ D3中,Hydrocortisone防止促炎刺激(TNFα的给药)引起的内皮屏障破坏,这可以证明是部分地维护occludin的水平。[1]

Hydrocortisone处理的树突细胞(DC)表现出降低的表达MHC II类分子,共刺激分子CD86和直流特异性标记CD83,以及一个大大减少的IL-12的分泌。Hydrocortisone处理的DC抑制产生IFN-γ而诱导IL-4的增加的释放,IL-5的没有变化。Hydrocortisone减少在树突状细胞的T细胞增殖。[2]

Hydrocortisone阻止TNF-α诱导糖萼的严重退化,增加冠脉阻力,提高血管渗漏和通透性,羟乙基淀粉并引起离体豚鼠心脏肥大细胞脱颗粒。[3]

在离体豚鼠心脏,Hydrocortisone降低缺血后的氧化应激,灌注压和漏出液形成。Hydrocortisone抑制多配体聚糖-1,硫酸乙酰肝素和乙酰透明质酸的缺血后脱落,以及从驻留肥大细胞组胺释放。[4]

Hydrocortisone增加IL-4诱导的胚系C epsilon转录水平增加两倍,并提供成熟Ç小量的mRNA转录所需的信号。在IL-4处理的B细胞中,Hydrocortisone诱导S mu-S epsilon缺失切换重组中,并支持序贯同型从IgM经由IgG4转换至IgE的模型。[5]

实验图片 检测方法 检测指标 实验图片 PMID
Western blot parkin / AIMP2 CREB 28366931
Immunofluorescence Glut2 29717618
Growth inhibition assay Cell viability 22829975
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05741762 Active not recruiting
Critical Illness|Adrenal Insufficiency|Septic Shock
Dr Adnan Agha|United Arab Emirates University
January 31 2023 --
NCT05607901 Recruiting
Dermatologic Disease
Tanta University
October 28 2022 Phase 2
NCT05324618 Completed
Atopic|Dermatitis
Ain Shams University|National Hepatology & Tropical Medicine Research Institute
May 15 2022 Phase 4

化学信息&溶解度

分子量 362.46 分子式

C21H30O5

CAS号 50-23-7 SDF Download Hydrocortisone SDF
Smiles CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 72 mg/mL ( (198.64 mM) ;DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Ethanol : 15 mg/mL (41.38 mM)

Water : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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