Ipilimumab (anti-CTLA-4)

目录号:A2001 别名: MDX-010, BMS-734016, Yervoy 中文名称:伊匹单抗

For research use only.
Not for use in humans.

Ipilimumab (anti-CTLA-4)是一种免疫调节单克隆抗体,靶向细胞表面抗原CTLA-4;也是一种免疫检查点抑制剂;MW:148 KD。

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客户使用Selleck该产品发表文献6篇:

质量管理及产品安全说明书

Immunology & Inflammation related抑制剂选择性比较

生物活性

产品描述 Ipilimumab (anti-CTLA-4)是一种免疫调节单克隆抗体,靶向细胞表面抗原CTLA-4;也是一种免疫检查点抑制剂;MW:148 KD。
靶点
CTLA-4 [1]
(Cell-free assay)
体外研究

Ipilimumab (anti-CTLA-4)是人源IgG1k单克隆抗体,与人源CTLA-4的胞外区域结合并具有高亲和力,是该复合体功能的抑制剂。Ipilimumab能引起T细胞的激活和增殖,淋巴细胞的浸润,从而导致肿瘤细胞的死亡。效应T细胞功能的增强、CD4+ Treg细胞和CD8+抑制细胞受到抑制,是ipilimumab发挥其治疗效果的关键[2]。Ipilimumab可增强ATC(活化T细胞)的增殖,BiAb-介导的肿瘤特异性细胞毒性和细胞因子的合成,而减弱Treg活性,IL-10释放减少[3]。Ipilimumab和nivolumab共同给药可增强效应T细胞和记忆T细胞反应,而不会不恰当地激活原始T细胞[4]

体内研究 Ipilimumab (anti-CTLA-4)在临床Ⅲ期试验中被发现对转移性黑素瘤可提高生存率,对10%-20%的患者的效果持久。ipilimumab的主要副作用是自身免疫[1]。Ipilimumab可通过抑制调节性T细胞(Treg)的免疫抑制活性,增强抗肿瘤免疫反应[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: Ipilimumab augments antitumor activity of bispecific antibody-armed T cells
    Cells: PBMC from healthy individuals; COLO356/FG (Pancreatic Cancer cell line) and Burkitt's lymphoma cell line
    Concentrations: 0, 0.5, 5.0, and 50 μg/mL
    Incubation Time: 10-14 days
    Method: hPBMC were stimulated with anti-CD3 monoclonal antibody with or without ipilimumab and expanded for 10-14 days. ATC were harvested and armed with anti-CD3 x anti-EGFR BiAb (EGFRBi) or anti-CD3 x anti-CD20 BiAb (CD20Bi) to test for redirected cytotoxicity against COLO356/FG pancreatic cancer cell line or Burkitt’s lymphoma cell line (Daudi).
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/25008236

    Objective: Ipilimumab potentiate IL-2 release in SEB-Stimulated Human PBMC
    Cells: hPBMC
    Concentrations: 10 μg/mL
    Incubation Time: 1 h
    Method: PBMC from normal healthy leukophoresis donors were seeded at 1×105 cells/well and stimulated with superantigen staphylococcal enterotoxin B (SEB) serially diluted 30-fold from 2.5 μg/mL. Ipilimumab, Nivolumab or huIgG4 isotype control was present at a spike concentration of 10 μg/mL. IL-2 secretion was measured by ELISA on day 3.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27610613

    Ipilimumab can apply to humanized mice (eg:NSG mice), peripheral blood and other related assays (Only for Reference)

动物实验:
- 合并
  • Objective: To investigate whether Ipilimumab and PD-1 antibody enhance the ability of T cells to control the outgrowth of EBV-induced lymphomas
    Animal Models: NSG mice (humanized mice) were injected (i.p.) with EBV infected human cord blood mononuclear cells (Commercially purchased from AllCells)
    Formulation: PBS
    Dosages: 100 μg, three times weekly
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27186886

    Objective: In vivo characterization of Ipilimumab T cell modulation and antitumor activity in a tumor bearing humanized NSG mouse model
    Animal Models: NSG mice (humanized mice) were injected human peripheral blood mononuclear cells (PBMCs).Then implanted subcutaneous with human A2058 melanoma and 786-O renal adenocarcinoma cell lines
    Formulation: --
    Dosages: twice weekly
    Administration: i.p.
    Reference: http://cancerimmunolres.aacrjournals.org/content/4/1_Supplement/A054.short

    Ipilimumab can apply to humanized mice (eg:NSG mice), peripheral blood and other related assays (Only for Reference)

抗体信息

CAS No. 477202-00-9
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID