PubMed: 29200902      Korean J Physiol Pharmacol

Saos2 cells were treated with 3 mM JPH203 for 24 h. Cell lysate was prepared and analyzed by immunoblotting as described in METHODS.

PubMed: 30241549      J Exp Clin Cancer Res

P-p70 S6 kinase, p-ERK and p-AKT levels were assayed in PTC cell lines. Cells were plated in triplicates using normal growth medium, after overnight incubation cells were washed and 0.125X EAA was added. Cells were treated with 10 μM JPH203 or DMSO as control and lysed after 24 h.

PubMed: 30784173      J Cell Mol Med

Activity of the two AA sensing pathways GCN2 and mTORC1 were analysed by immunoblot in the absence or presence of either 20 or 30 µmol/L of JPH203. ERK1/2 served as a loading control (the experiment presented here is representative of at least three independent experiments)

PubMed: 29200902      Korean J Physiol Pharmacol

Saos2 and FOB cells were treated with various concentrations of JPH203 (A, Saos2 cells; B, FOB cells) for 1-4 days. The cell viabilities were determined by MTT assays. The percentage cell viability was calculated as a ratio of the optical density at 570 nm of JPH203 treated cells and untreated control cells. The control cells were measured in the absence of JPH203 treatment. Each data point represents the mean±S.E.M. of four experiments. *p<0.05 vs. control, **p<0.01 vs. control and ***p<0.001 vs. control.