Liproxstatin-1

For research use only. Not for use in humans.

目录号：S7699

Liproxstatin-1 Chemical Structure

CAS No. 950455-15-9

Liproxstatin-1是一种有效的ferroptosis抑制剂，IC50为22 nM。

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10mM (1mL in DMSO) RMB 1203.93 现货
RMB 1199.01 现货
RMB 3903.06 现货
RMB 8170.23 现货
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客户使用Selleck生产的Liproxstatin-1发表文献101篇：

产品安全说明书

Ferroptosis抑制剂选择性比较

生物活性

产品描述 Liproxstatin-1是一种有效的ferroptosis抑制剂，IC50为22 nM。
靶点
ferroptosis [1]
(Cell-free assay)
22 nM
体外研究

Liproxstatin-1能够在低纳摩尔范围内抑制铁死亡和Gpx4−/−细胞的增殖（IC50，22 nM）。在Gpx4−/−细胞中，liproxstatin-1（50 nM）可完全阻断脂质过氧化作用 。liproxstatin-1（200 nM）可以浓度依赖性克服FINs,如BSO （10 µM）、erastin（1 µM）和RSL3（0.5 µM）诱发的细胞死亡，但不能克服staurosporine（0.2 µM）和 H2O2（200 µM）诱发的细胞死亡。[1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC-5 lung fibroblast cells MU\EUmRKQiCFeYTveI95cWOrdImgZYdicW6|dDDoeY1idiCPUlOtOUBtfW6pIH\pZpJw[myjc4SgZ4VtdHNuIFnDOVAhRSB7LkG0JO69VQ>? NFjZWFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzODl2N{WwM{c,S2iHTVLMQE9iRg>?

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
GPX4 / β-actin; 

PubMed: 33602915     


IEC6 cells were co-cultured with 100 μM compound 5 or 50 nM liproxstatin-1 for 4 h (ROS and GSH experiments) or 24 h (survival experiment). C) WB images showed expression of GPX4 protein in IEC6 cells.

GRP78 / CHOP / Actin; 

PubMed: 29383150     


The combinatorial treatment of ferroptotic agent and TRAIL promotes apoptosis via ER stress, but not lipid peroxidation in HCT116 cells. Cells were treated with 50 μM ART for 24 h in the absence/presence of DFO (100 μM), ferrostatin-1 (Fer-1, 10 μM), or liproxstatin-1 (Lip-1, 2 μM).

GRP78 / CHOP / Actin; 

PubMed: 29383150     


The combinatorial treatment of ferroptotic agent and TRAIL promotes apoptosis via ER stress, but not lipid peroxidation in HCT116 cells. Cells were treated with 50 μM Erastin for 24 h in the absence/presence of DFO (100 μM), ferrostatin-1 (Fer-1, 10 μM), or liproxstatin-1 (Lip-1, 2 μM).

NeuN / Syn / SNAP / GFAP / Actin / Tubulin; 

PubMed: 28212525     


Western blots showing attenuated neurodegeneration (NeuN, Synaptophysin, SNAP25) and neuroinflammation (GFAP) in HC of Gpx4BIKO-VED mice treated with the ferroptosis inhibitor liproxstatin-1 compared to Gpx4BIKO-VED mice treated with vehicle (DMSO) at 1 week post TAM treatment (n=5 for all groups).

β-actin / COX2 / GPx4; 

PubMed: 30737476     


Liproxstatin-1 inhibits lipid peroxidation and cell death in vitro. Caco-2 cells were pretreated with liproxstatin-1 (200 nM) for 12 h before being subjected to H/R (H, 12 h of hypoxia; R, 2 h of reoxygenation). All samples were collected after H/R. a GPx4 and COX2 expression in cells were detected by western blotting (n = 3).

β-actin / COX2 / GPx4; 

PubMed: 30737476     


Liproxstatin-1 ameliorates ferroptosis and intestinal injury in vivo. Mice were treated with liproxstatin-1 (10 mg/kg) by intraperitoneal injection 1 h before ischemia and then subjected to ischemia/reperfusion (I, 45 min of ischemia; R, 30 min of reperfusion) or sham surgery (sham). All samples were collected after I/R. a GPx4 and COX2 levels were assessed by western blotting (n = 3).

COX2 / GPx4 / GAPDH; 

PubMed: 33889074     


Liproxstatin-1 inhibits ferroptosis after stroke. (A) The protein levels of COX2 and GPx4 in peri-infarct region of sham group, MCAO group, sham + Lip group and MCAO + Lip group at 24 h after cerebral ischemic reperfusion, respectively.

33602915 29383150 28212525 30737476 33889074
Growth inhibition assay
Cell viability; 

PubMed: 31641008     


(B-C) Two ferroptosis inhibitors rescued cystine deprivation-induced cell death. TOV-21G cells were seeded in 10% (20 μM of cystine) or 20% (40 μM of cystine) of full DMEM media supplied with or without either 200 nM liproxstatin-1 (B) or 2 μM ferrostatin-1.

31641008
Immunofluorescence
mitochondrial Fe2+; 

PubMed: 33343803     


Fluorescence microscopic analysis for detecting mitochondrial Fe2+ and lipid peroxidation in neomycin-damaged HEI-OC1 cells. Representative images of Mito-FerroGreen staining in the (a) control, (b) Lip-1, (c) neomycin (Neo), and (d) Lip-1+neomycin cotreatment (Lip-1+Neo); scale bars indicate 10 μm.

NeuN / ACSL4; 

PubMed: 33314758     


(F) Immunofluorescence analysis performed using antibodies specific for ACSL4 (red), a neuronal marker (green), and DAPI nuclear stain (blue). Scale bar = 100 μm. (G) Quantification analysis of the relative fluorescence intensity of ACSL4. **p < 0.0001 vs. Sham group; ## p = 0.5136, @@ p = 0.8177 vs. SAH + Vehicle group; results analyzed using unpaired t‐test, n = 6. All data are shown as mean ± SEM. Mean values of the Sham group were normalized to 1.0.

β-actin / COX2 / GPx4; 

PubMed: 30737476     


Cell lipid peroxidation were detected by BODIPY 581/591 C11 staining using fluorescence microscopy (scale bar = 100 μm) and 12-HETE, 15-HETE, and LPO assay kits (n = 6).

33343803 33314758 30737476
体内研究 与溶剂对照组相比，Liproxstatin-1可显著地延长生存期，抑制管状细胞中铁死亡，减轻缺血/再灌注诱发的肝损伤中的组织损伤。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验：

[1]
- 合并
  • Cell lines: Gpx4−/−细胞
  • Concentrations: ~200 nM
  • Incubation Time: ~72 h
  • Method:

    经样品处理后，根据制造商的指示使用AquaBluer检测不同时间点的细胞存活率。另外，使用细胞毒性活性检测试剂盒（LDH）测定乳酸脱氢酶释放量来检测细胞死亡。


    (Only for Reference)
动物实验：

[1]
- 合并
  • Animal Models: GreERT2; Gpx4fI/fI小鼠
  • Dosages: 10 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 68 mg/mL (199.5 mM)
Water Insoluble
Ethanol '21 mg/mL warmed
体内 从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)：
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
 3mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 340.85
化学式

C19H21ClN4
CAS号 950455-15-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量 只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO % % Tween 80 % ddH2O
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

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    体积
    分子量

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稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

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    V1
    C2
    V2

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

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    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量：

总分子量：g/mol

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID