Cisplatin induces Notch signaling in mouse kidneys. A‐D, Western blot images and bar graphs showing Dll1 and Jag1 protein expression levels in the kidneys of mice 5 days after treatment with Captisol (vehicle control; IP) and cisplatin (15 mg/kg; IP). E and F, Western blot images and bar graphs illustrating N1ICD protein expression levels in the kidneys of Captisol‐, cisplatin‐, and DAPT (15 mg/kg; IP) + cisplatin‐treated mice.
Cisplatin increases Dll1 and N1ICD but decreases Jag1 protein expression in HK‐2 cells. A‐F: Western blot images and bar graphs showing A and B, Dll1; C and D, N1ICD; and E and F, Jag1 protein expression levels in HK‐2 cells treated (12 h) with Captisol (vehicle control) and cisplatin (30 μmol L−1).
Captisol (vehicle) or 120 mg/kg MK-2206 were administered to CALRdel52 mice by oral gavage for four weeks, five days a week, beginning 20 weeks post-transplant. Hematopoiesis was analyzed at 24 weeks post-transplant. h) Western blot evaluating the levels of AKT, p-AKT and cyclinD3 in treated mice. Grb2 is included as a loading control.
Antagomir-9-3p restores AZD8055-suppressed growth and survival of tumor xenografts. (C) MDA-MB-231 xenografts treated as described in A were either analyzed for the indicated proteins by Western blotting.
DAPT protects against renal morphological changes induced by cisplatin. A, kidney‐to‐terminal body weight ratio (n = 11 each; data were obtained from all groups, except mice used for multiphoton microscopy); B, images (PAS staining) and C, average tubular injury score (n = 4 each) in Captisol (vehicle control; IP)‐, cisplatin (15 mg/kg; IP)‐, DAPT (15 mg/kg; IP)‐, and DAPT + cisplatin‐treated mice. Average tubular injury scores in Captisol‐ and DAPT‐treated mice were zero. Arrows identify tubular injury in the form of vacuolar degeneration and necrosis.
Quantitative confocal imaging of immunostained CC sections for MOG, GSTπ, and pCXCR4. Data are expressed as the mean positive area (MOG and pCXCR4) or mean number of cells (GSTπ) for mice treated with captisol, CCX771 alone, CCX771 and saline, and CCX771 and AMD3100.