Quizartinib (AC220)

For research use only. Not for use in humans.

目录号:S1526 中文名称:奎扎替尼

Quizartinib (AC220) Chemical Structure

CAS No. 950769-58-1

Quizartinib (AC220)是一种二代FLT3抑制剂,作用于Flt3(ITD/WT),在MV4-11和 RS4;11细胞中IC50分别为1.1 nM/4.2 nM,作用于Flt3比作用于KIT,PDGFRA,PDGFRB,RET,和CSF-1R选择性高10倍。Quizartinib (AC220)可诱导肿瘤细胞的凋亡。Phase 3。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2891.02 现货
RMB 1729.76 现货
RMB 7933.51 现货
RMB 20229.3 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Quizartinib (AC220)发表文献94篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 Quizartinib (AC220)是一种二代FLT3抑制剂,作用于Flt3(ITD/WT),在MV4-11和 RS4;11细胞中IC50分别为1.1 nM/4.2 nM,作用于Flt3比作用于KIT,PDGFRA,PDGFRB,RET,和CSF-1R选择性高10倍。Quizartinib (AC220)可诱导肿瘤细胞的凋亡。Phase 3。
特性 AC220是二代FLT3抑制剂,且作为临床FLT3抑制剂的第一候选药。
靶点
FLT3 (ITD) [1]
(MV4-11 cells)
FLT3 (WT) [1]
(RS4;11 cells)
1.1 nM 4.2 nM
体外研究

AC220是最有效的FLT3选择性抑制剂,可用于治疗急性骨髓性白血病(AML)。AC220在生化和细胞实验中具有低纳摩尔效果和特别的激酶选择性,抑制大部分人类蛋白激酶时具有高选择性。AC220是治疗急性骨髓性白血病(AML)的新型FLT3抑制剂。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60/VCR NFfZNJVHfW6ldHnvckBCe3OjeR?= Mn\1NE4yNTFyIN88US=> NEfLWlI{OCCvaX6= MWHlcohidmOnczD1dJRic2Vib3[gd5Vje3S{YYTld{Bw\iCDQlPHNkBidmRiQVLDRlEhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI> NFnTdmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2O|E4Pyd-MkO5OlcyPzd:L3G+
K562/ABCB1 NWfNSmM1TnWwY4Tpc44hSXO|YYm= NFWzW3gxNjFvMUCg{txO M2DIWlMxKG2rbh?= Mmmw[Y5p[W6lZYOgeZB1[WunIH;mJJN2[nO2cnH0[ZMhd2ZiQVLDS|Ih[W6mIFHCR2IyKGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz M{jselxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[3NVc4Lz5{M{m2O|E4PzxxYU6=
8226/MR20  NVm2XJB7TnWwY4Tpc44hSXO|YYm= M3uwNlAvOS1zMDFOwG0> NXv4[4lZOzBibXnu NYfjenV5\W6qYX7j[ZMhfXC2YXvlJI9nKHO3YoP0doF1\XNib3[gRWJETzJiYX7kJGFDS0JzIHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{ M37VOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[3NVc4Lz5{M{m2O|E4PzxxYU6=
K562/ABCG2 MV7GeY5kfGmxbjDBd5NigQ>? NFrCVXYxNjFvMUCg{txO NHHVOZg{OCCvaX6= NFLUWIVmdmijbnPld{B2eHSja3Wgc4Yhe3Wkc4TyZZRmeyCxZjDBRmNIOiCjbnSgRWJESjFiaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDtZY5v\XJ? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
MCF-7 FLV1000 NEn5NoRMcW6jc3WgRZN{[Xl? NYLtVHZKOOLCk{OwJOK2VQ>? Mn3TOUBucW5? MoXI[IVkemWjc3XzJHsyOjWLXT3JRWFRKHCqb4TvcIFj\Wyrbnegc4YhSUKFQkGgZZQhUUN3MDDv[kA{NjNizszN MmeyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
MCF-7 FLV1000 NEHQN5JMcW6jc3WgRZN{[Xl? MkjNNQKBmzNyINM1US=> NWrnfYloPSCvaX6= MYLk[YNz\WG|ZYOgX|EzPUmfLVnBRXAheGixdH;sZYJmdGmwZzDv[kBCSkOEMjDheEBKSzVyIH;mJFAvODdizszN Mni0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkexO|coRjJ|OU[3NVc4RC:jPh?=
K562/ABCG2 MlnCR4VtdCCYaXHibYxqfHliQYPzZZl{ NVnZ[3Z3OC5zL{CuOU8yKML3TR?= NYjDb4tiQTZiaB?= NGnOPWd{\W6|aYTpfoV{KEt3NkKvRWJETzJiY3XscJMhfG9ibXn0c5hidnS{b37lJJRweG:2ZXPhcuKh NXvvWpNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OlcyPzdpPkKzPVY4OTd5PD;hQi=>
8226/MR20 MVPD[YxtKF[rYXLpcIl1gSCDc4PhfZM> MnXKNE4yKML3TR?= MX[5OkBp Mkm5d4Vve2m2aYrld{BMPTZ{L1HCR2czKGOnbHzzJJRwKG2rdH;4ZY51em:wZTD0c5BwfGWlYX9CpC=> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4N{G3O{c,OjN7NkexO|c9N2F-
HMC1.1 NEO1ZWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7kTWM2OD1zNDDuUS=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
HMC1.2 NYrOVGxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTF5Mkegcm0> M1zpdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
p815 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTR2NTDuUS=> NXf2[YVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Kasumi-1 NUfSZ5Q6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV:2V4ZbUUN3ME2zOkBvVQ>? NIfvbJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
M-07e + SCF NIj1[|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTd5IH7N NH\YbHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
EOL-1 MnW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPaTWM2OD1zIH7N NV;sfIpnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MV4;11 M1;0UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\GW4l1UUN3MEygNUBvVQ>? M4r2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
MOLM14 Ml3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfmUnpjUUN3MEygNUBvVQ>? Mmn1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Pat.221 M3nMOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzuPWxQUUN3ME22O|Uhdk1? NV\oU4xrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Pat.279 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHPXIFKSzVyPUO0N|Qhdk1? MofLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Pat.299 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTd{NEigcm0> NFr2UZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.305 M1jvUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\E[GlEPTB;N{C3PUBvVQ>? Mn3BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Pat.375 M4jHeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTVyMzDuUS=> M4eybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
Pat.379 MoL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXzTWM2OD16ME[gcm0> NETvcoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.368 MoHaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7vd3RsUUN3ME2yO|AxKG6P NUP1ZYVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
Pat.601 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjDTWM2OD1zMUWzJI5O M1fmfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
HMC1.1 MojjRZBweHSxc3nzJGF{e2G7 M3rzOWlEPTB;M{Ggcm0> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
p815 NILTe3JCeG:ydH;zbZMhSXO|YYm= M33abGlEPTB;M{SxJI5O MlP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
Kasumi-1 NFnGXHZCeG:ydH;zbZMhSXO|YYm= Ml\ZTWM2OD14NzDuUS=> MoLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUezNVcoRjJ|NEm3N|E4RC:jPh?=
M-07e + SCF NVLkVGJFSXCxcITvd4l{KEG|c3H5 M4[5R2lEPTB;N{igcm0> NU\JT3o4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
EOL-1 M12yTmFxd3C2b4Ppd{BCe3OjeR?= MnvvTWM2ODxiMTDuUS=> M{iz[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
MV4;11 MnO0RZBweHSxc3nzJGF{e2G7 Mn[wTWM2OD1{IH7N M1vtS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3N|E4Lz5{M{S5O|MyPzxxYU6=
MOLM14 MYXBdI9xfG:|aYOgRZN{[Xl? NWWy[o5DUUN3ME2zJI5O NInxNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
GIST822 MmnnRZBweHSxc3nzJGF{e2G7 NIjL[nhKSzVyPUGwPUBvVQ>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{OxO{c,OjN2OUezNVc9N2F-
Pat.368 Mnf2RZBweHSxc3nzJGF{e2G7 M1rqcWlEPTB;Mkm5PEBvVQ>? NGjHfnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|MyPyd-MkO0PVc{OTd:L3G+
Pat.601 NG\uO4FCeG:ydH;zbZMhSXO|YYm= MXfJR|UxRTh5NjDuUS=> NVrJeph3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVc{OTdpPkKzOFk4OzF5PD;hQi=>
MV4-11 MmLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXy3NkBp MnqxTWM2OD1yLkOgcm0> Mn;FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MUK5N|EoRjJ|NEGyPVMyRC:jPh?=
MOLM-14 NVrubm5tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWC3NkBp NUjaXHNNUUN3ME2wMlEhdk1? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMkmzNUc,OjN2MUK5N|E9N2F-
SEM-K2 M{TkUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3ZUVA4OiCq MkfoTWM2OD1yLkSgcm0> NHToU5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
RS4;11 MlqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV63NkBp MlvwTWM2OD5zMDywNFAhdk1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMkmzNUc,OjN2MUK5N|E9N2F-
THP-1 NX20fnh6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHGdWtZPzJiaB?= MmH0TWM2OD5zMDywNFAhdk1? NGPFdos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
MV4-11 NVjPR|BRSXCxcITvd4l{KEG|c3H5 MmnLPE8zPCCq MYjpcoR2[2W|IIPp[45q\mmlYX70JIFv\CCmb4PlMYRmeGWwZHXueEBRSVKSIHPs[YF3[WenIHHu[EBi[2O3bYXsZZRqd25ib3[gd5VjNTKQIFTORS=> NHm0R4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNlk{OSd-MkO0NVI6OzF:L3G+
MOLM-14 NVXFS5ozSXCxcITvd4l{KEG|c3H5 NVT5WG5TQC9{NDDo NFTxVJhqdmS3Y3XzJJNq\26rZnnjZY51KGGwZDDkc5NmNWSncHXu[IVvfCCSQWLQJINt\WG4YXflJIFv\CCjY3P1cZVt[XSrb36gc4Yhe3WkLULOJGRPSQ>? M{DYSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGyPVMyLz5{M{SxNlk{OTxxYU6=
SEM-K2 MnTZRZBweHSxc3nzJGF{e2G7 NXm3ZoFxQC9{NDDo MXXpcoR2[2W|IIPp[45q\mmlYX70JIFv\CCmb4PlMYRmeGWwZHXueEBRSVKSIHPs[YF3[WenIHHu[EBi[2O3bYXsZZRqd25ib3[gd5VjNTKQIFTORS=> NVLSN29xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVI6OzFpPkKzOFEzQTNzPD;hQi=>
AML MX7DfZRwfG:6aXPpeJkh[XO|YYm= MlvCR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRW1NKGOnbHzzJIl{d2yjdHXkJIZzd21icnXsZZB{\WRiYXP1eIUhdXmnbH;p[EBt\XWtZX3pZUBx[XSrZX70JIhiemKxcnnu[{BHVFR|IFnUSEBufXSjdHnvckBjgSCPVGSgZZN{[XluIFnDOVAhRSByLkCwNFMh|ryPLh?= NIDjR5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
MV4-11 NXvDV5kzS3m2b4TvfIlkcXS7IHHzd4F6 MXG3NkBpenN? NHXud5BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGOnbHygeIl1\XJvYnz1[UBie3OjeTygTWM2OCB;IECuNFAxPTZizszNMi=> NIfxV5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
blast cells Mn71SpVv[3Srb36gZZN{[Xl? M3XtPFIhcHK| NHX3WYZKdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCCjdYTvdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIHLsZZN1KGOnbHzzJIFnfGW{IEKgbJJ{KGK7IHXs[YN1em:laHXtbYx2dWmwZYPj[Y5k\SxiSVO1NEA:KDBwMECwPEDPxE1w M17SclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NkW0OFA5Lz5zOU[1OFQxQDxxYU6=
RS4-11 M1vUNmZ2dmO2aX;uJIF{e2G7 NFTXT5kzKGi{cx?= MoiyTY5pcWKrdHnvckBw\iCITGSzJGlVTCCvdYThcpQh[XW2b4Doc5NxcG:{eXzheIlwdiCrbjDoeY1idiCUU{StNVEh[2WubIOgZYZ1\XJiMjDodpMh[nliZXzlZ5Rzd2OqZX3pcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xODFzIN88UU4> NV;VN3hKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2OVQ1ODhpPkG5OlU1PDB6PD;hQi=>
AML MkPRSpVv[3Srb36gZZN{[Xl? MULJcohq[mm2aX;uJI9nKE[OVEOgZZV1d3Cqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBCVUxiY3XscJMhcXOxbHH0[YQh\nKxbTDy[YxieHOnZDDhZ5V1\SCveXXsc4llKGyndXvlcYliKHCjdHnlcpQh[nliV3XzeIVzdiCkbH;0eIlv\yxiSVO1NEA:KDBwMECyJO69VS5? MkjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl4NUS0NFgoRjF7NkW0OFA5RC:jPh?=
RS4-11 NXHC[nBPTnWwY4Tpc44h[XO|YYm= NYPBe|RyOiCqcoO= NF[zeFRKdmirYnn0bY9vKG:oIF\MWFMh[XW2b4Doc5NxcG:{eXzheIlwdiCrbjDoeY1idiCUU{StNVEh[2WubIOgZYZ1\XJiMjDodpMh[nliZXzlZ5Rzd2OqZX3pcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xODR{IN88UU4> NVXQRlljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2OVQ1ODhpPkG5OlU1PDB6PD;hQi=>
MV4-11 NIHqSWxCdnSrdIXtc5Ih[XO|YYm= MWKxNEBu\y:tZx?= MYi2NEBl[Xm| M1jae2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{B5\W6xZ4Lh[pRm\CCvb4Xz[UBud2SnbDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIT1cY9zKHKnZ4Lve5RpKGG2IHH0JFExKG2pL3vnMEBxdyB4MDDkZZl{KHCxc4SgeJJm[XSvZX70 NXz5PWN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2OVQ1ODhpPkG5OlU1PDB6PD;hQi=>
MV4-11 NXi4VHEzSW62aYT1cY9zKGG|c3H5 M4DTcVEhdWdxa3ev[IF6 M2PB[lMxKGSjeYO= NVnNSlFTSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUXY1NTFzIHPlcIx{KHinbn;ndoFnfGWmIH3veZNmKGKxbnWgcYFzem:5IHXu[5Ji\nSvZX70JI1w\GWuIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI1wfXOnIHzp[oUhe3CjbjDheEAyKG2pL3vnM4RigSxicH:g[o9zKDNyIHThfZMhdWWjc4Xy[YQhd25iZHH5JFc4KHCxc4Sg[I9{\Q>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTZ3NESwPEc,OTl4NUS0NFg9N2F-
MV4-11 MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M1KzVVczKGi{cx?= NEezWY9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{Bi\nSncjC3NkBpenNiYomgZ4VtdCC2aYTldk1jdHWnIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gNE4xODB3NjFOwG0v Mn\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5NUSxPVkoRjF7N{W0NVk6RC:jPh?=
HEK293 MnzESpVv[3Srb36gZZN{[Xl? NYnXSmpISmmwZHnu[{Bi\m[rbnn0fUB1dyCITGSzJINifGGueYTpZ{Bld22jaX6g[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBkd22yZYTpeIl3\SCkaX7kbY5oKGG|c3H5MEBM\CB;IECuNFAyPiEQvF2u NX7oRWdXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3OVQyQTlpPkG5O|U1OTl7PD;hQi=>
MV4-11 MkjDRY51cXS3bX;yJIF{e2G7 MXKxJI1oN2up M3TESFI5KGSjeYO= NIqyc4ZCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNWlQuOTFiY3XscJMhgGWwb3fyZYZ1\WRiaX6gZZRpgW2rYzDueYRmKG2xdYPlJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geJVud3JiZ4Lve5RpKGG2IEGgcYcwc2duIIDvJHFFKG[xcjCyPEBl[Xm|IH3lZZN2emWmIHT1dolv\yCmb4PpcocheGW{aX;k NVLmXGJIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3OVQyQTlpPkG5O|U1OTl7PD;hQi=>
MV4-11 M37WWGFvfGm2dX3vdkBie3OjeR?= MkfJN{Bu\y:tZx?= Mm[zNlgh\GG7cx?= MnzXRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTW[0MVEyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIHH0bJlucWNiboXk[UBud3W|ZTDhd5Nme3OnZDDhd{B1fW2xcjDndo94fGhicnXndoV{e2mxbjDheEA{KG2pL3vnMEBxdyCTRDDmc5IhOjhiZHH5d{Bu\WG|dYLl[EBi\nSncjDkc5NqdmdicHXybY9l NXTk[IhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3OVQyQTlpPkG5O|U1OTl7PD;hQi=>
MV4-11 M13wXmFvfGm2dX3vdkBie3OjeR?= NV3IZ|FVOTBibXevb4c> MnmzNlgh\GG7cx?= NV:yWWl{SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUXY1NTFzIHPlcIx{KHinbn;ndoFnfGWmIHnuJIF1cHmvaXOgcpVl\SCvb4Xz[UBie3Onc4Pl[EBieyC2dX3vdkBoem:5dHigdoVoemW|c3nvckBifCBzMDDt[{9s\yxicH:gVWQh\m:{IEK4JIRigXNibXXhd5Vz\WRiYX\0[ZIh\G:|aX7nJJBmemmxZB?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd3NEG5PUc,OTl5NUSxPVk9N2F-
MV4-11 NYPubWliSW62aYT1cY9zKGG|c3H5 M3\MWVMhdWdxa3e= MmDJNlgh\GG7cx?= MXHBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPVkStNVEh[2WubIOgfIVvd2e{YX\0[YQhcW5iYYTofY1q[yCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIIT1cY9zKH[xbIXt[UBifCB|IH3nM4toNCCybzDRSEBnd3JiMkig[IF6eyCvZXHzeZJm\CCjZoTldkBld3OrbnegdIVzcW:m MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd3NEG5PUc,OTl5NUSxPVk9N2F-
MV4-11 M{KwRWFvfGm2dX3vdkBie3OjeR?= MWGxNEBu\y:tZx?= M4HRSVI5KGSjeYO= MlrVRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTW[0MVEyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIHH0bJlucWNiboXk[UBud3W|ZTDhd5Nme3OnZDDhd{B{fXCycnXzd4lwdiCxZjD0eY1weiC4b3z1cYUh[XRiMUCgcYcwc2duIIDvJHFFKG[xcjCyPEBl[Xm|IH3lZZN2emWmIHHmeIVzKGSxc3nu[{Bx\XKrb3S= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd3NEG5PUc,OTl5NUSxPVk9N2F-
MV4-11 M4DPRmFvfGm2dX3vdkBie3OjeR?= M3X0dlExKG2pL3vn MYmyPEBl[Xm| M3[xdmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDheIh6dWmlIH71[IUhdW:3c3WgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0eY1weiCpcn;3eIgh[XRiMUCgcYcwc2duIIDvJHFFKG[xcjCyPEBl[Xm|IH3lZZN2emWmIHHmeIVzKDN{IHThfZMheG:|dHTvd4U> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd3NEG5PUc,OTl5NUSxPVk9N2F-
MOLM13 NET4UmJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXnhe2xMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNU2xOOTNiY3XscJMh\XiycnXzd4lv\yCITGSzMWlVTCCvdYThcpQtKEeLNUCgQUAxNjByNDFOwG0v NIDEWGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyOlk{OSd-MkK3NlY6OzF:L3G+
MV411 M2ToO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUOx[XMxPDhiaILz M3HWOGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1XPDFzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEeLNUCgQUA{NjNzIN88UU4> NILhSJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGwPFA4QSd-MkWxNFgxPzl:L3G+
THP1 MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVXVUGdHPDhiaILz MV3Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDUTHAyKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGdKPTBiPTC1MlU4PCEQvF2u NWPOXohkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNFgxPzlpPkK1NVA5ODd7PD;hQi=>
HL60 NXX2SHpxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIrlWYQ1QCCqcoO= MkK1S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG|c3H5MEBIUTVyIE2gPE4xOTRizszNMi=> MkPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEiwO|koRjJ3MUC4NFc6RC:jPh?=
32D MYnGeY5kfGmxbjDhd5NigQ>? M1r0S|czKGi{cx?= NUXleZc{UW6qaXLpeIlwdiCxZjDoeY1idiCITGSzJGlVTCCvdYThcpQh\XiycnXzd4VlKGmwIH3veZNmKDN{RDDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjByMECxJO69VS5? NW\1TVF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
MV4-11 MWfGeY5kfGmxbjDhd5NigQ>? MVy3NkBpenN? MkTFTY5pcWKrdHnvckBw\iCITGSzJGlVTCCqb33vfplod3W|IH31eIFvfCCrbjDoeY1idiCPVkStNVEh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDHTVUxKD1iMD6wNFMh|ryPLh?= M4TVSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
MOLM-13 MlnXSpVv[3Srb36gZZN{[Xl? M1uyRVczKGi{cx?= NETQ[mRKdmirYnn0bY9vKG:oIF\MWFMhUVSGIHjleIVzd3q7Z3;1d{BufXSjboSgbY4hcHWvYX6gUW9NVS1zMzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjByNDFOwG0v M2XWclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
32D NXu4ZnlUTnWwY4Tpc44h[XO|YYm= NVrMfGhUPzJiaILz Mn\BTY5pcWKrdHnvckBw\iCmcoXnJJJme2m|dHHueEBpfW2jbjDGUHQ{KEmWRDDEPFM2YSCvdYThcpQh\XiycnXzd4VlKGmwIH3veZNmKDN{RDDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjBzNjFOwG0v NGDpV|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
32D Mn\PSpVv[3Srb36gZZN{[Xl? MmL2O|IhcHK| MlO1TY5pcWKrdHnvckBw\iCmcoXnJJJme2m|dHHueEBpfW2jbjDGUHQ{KEmWRDDEPFM2XiCvdYThcpQh\XiycnXzd4VlKGmwIH3veZNmKDN{RDDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjB{NzFOwG0v NX7SUIFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
Sf9 NVO4RmFWTnWwY4Tpc44h[XO|YYm= NE\hU2Y1KGi{cx?= M3H1R2lvcGmkaYTpc44hd2Zid3ns[EB1gXCnIFfTWE11[WepZXSgSmxVOyCtaX7hd4Uh\G:vYXnuJEh[PTZ5IITvJHM6QTNrIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBU\jliY3XscJMhfXOrbnegTIVzOiCyZYD0bYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiB2IHjyd{BjgSCNaX7hd4UuT2yxIHHzd4F6NCCLQ{WwJF0hOC5yNkmg{txONg>? NUHQeXBPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
32D M3rTbGZ2dmO2aX;uJIF{e2G7 M1vlXFczKGi{cx?= MX\Jcohq[mm2aX;uJI9nKGS{dXegdoV{cXO2YX70JIh2dWGwIF\MWFMhUVSGIFS4N|VHKG23dHHueEBmgHC{ZYPz[YQhcW5ibX;1d4UhOzKGIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO|IhcHK|IHL5JG1VWyCjc4PhfUwhT0l3MDC9JFAvOTVizszNMi=> NILuWZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
32D NYXmfmdtTnWwY4Tpc44h[XO|YYm= M3zQR|czKGi{cx?= NX7mTlc4UW6qaXLpeIlwdiCxZjDkdpVoKHKnc3nzeIFvfCCqdX3hckBHVFR|IFnUSEBHPjlzTDDteZRidnRiZYjwdoV{e2WmIHnuJI1wfXOnIEOySEBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFfJOVAhRSByLkKyJO69VS5? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
Sf9 NHvDU|ZHfW6ldHnvckBie3OjeR?= NFvKeI0yOjBibXnudy=> MWDJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IFfTWE11[WepZXSgWmVITlJ{IHvpcoF{\SCmb33hbY4hMFZ5OEmgeI8hXjF|NU[pJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKGmwc3XjeEBU\jliY3XscJMhfXOrbnegdI9tgUeudUS6WJlzKHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDF{MDDtbY5{KGK7IFvpcoF{\S2JbH:gZZN{[XluIFnDOVAhRSByLkSzJO69VS5? M1XzUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
RS4:11 MmLPSpVv[3Srb36gZZN{[Xl? MnG1O|IhcHK| MUnJcohq[mm2aX;uJI9nKHerbHSgeJlx\SCqb33vfplod3W|IF\MWFMhcW5iaIXtZY4hWlN2OkGxJINmdGy|IHHzd4V{e2WmIHHzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiR1m1NEA:KDJwMjFOwG0v MnnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
MV4-11 NV[0R5FsSW62aYT1cY9zKGG|c3H5 NG\5VI8yOCCvZz;r[y=> MYm0PEBl[Xm| M3y0TGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDueYRmKG2xdYPlJIF{e2W|c3XkJIF{KHS3bX;yJJJm\3Knc4Ppc44h[XRiMUCgcYcwc2duIIDvJJFlKGGmbXnubZN1\XKnZDDvckBl[XliMTD0c{A2KG2nYYP1doVlKGS3cnnu[{A1QCCmYYnzJJBwe3RibHHzeEBld3On MkToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
MV4-11 NVzLR|ZUSW62aYT1cY9zKGG|c3H5 NE\JZXkyOCCvZz;r[y=> Mn\tOFgh\GG7cx?= MlLjRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTW[0MVEyKGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71[IUhdW:3c3WgZZN{\XO|ZXSgZZMhfHWvb4KgdoVoemW|c3nvckBifCBzMDDt[{9s\yxicH:gdYQh[WSvaX7pd5RmemWmIH;uJIRigSB6IITvJFEzKG2nYYP1doVlKGS3cnnu[{A1QCCmYYnzJJBwe3RibHHzeEBld3On MojrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
MV4-11 NVPGUZF3S3m2b4TvfIlkcXS7IHHzd4F6 NUXSTYg3PzJiaILz MYjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNWlQuOTFiY3XscJMhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xODB4ODFOwG0v MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNEKzNVcoRjJ4MUSyN|E4RC:jPh?=
Sf21 MUDGeY5kfGmxbjDhd5NigQ>? Ml\lNVUhdWmwcx?= MnHrTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCITGSzJIV5eHKnc4Pl[EBqdiCrboPlZ5QhW2Z{MTDj[YxteyCycnXpcoN2[mG2ZXSg[o9zKDF3IH3pcpMh\m:ubH;3[YQh[nlicH;sfUhIdHV8VInyLUB{fWK|dILheIUh[WSmaYTpc44h\m:{IEOwJI1qdnNiaX6gdJJme2WwY3Wgc4YhY2ejbX3hMVMzWF2DVGCgZpkhXFJvRmLFWEBie3OjeTygTWM2OCB;IECuNFI3ODhizszNMi=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6N{i2NEc,OjdzOEe4OlA9N2F-
MV4-11 M1rUbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVe0PEBpenN? M{m0N2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1XPC1zMTDj[YxteyCneIDy[ZN{cW6pIF\MWFMuUVSGIHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFfJOVAhRSBzLkGwN{DPxE1w M2T4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi3PFYxLz5{N{G4O|g3ODxxYU6=
HL60 MnLXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkDQOFghcHK| Mo[0S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGw3OCClZXzsd{BmgHC{ZYPzbY5oKHerbHSgeJlx\SCITGStN{Bi\nSncjC0PEBpenNiYomgXHRVKGG|c3H5MEBIUTVyIE2gNU41QDhizszNMi=> MnTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOEe4OlAoRjJ5MUi3PFYxRC:jPh?=
THP1 NV\vTohxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV:0VHpuPDhiaILz MXzHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDUTHAyKGOnbHzzJIV5eHKnc4Ppcochf2muZDD0fZBmKE[OVD2zJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGdKPTBiPTCyMlEzPSEQvF2u MmfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOEe4OlAoRjJ5MUi3PFYxRC:jPh?=
K562 NYrVcWVET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PZVFQ5KGi{cx?= NILoZpRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBMPTZ{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEeLNUCgQUAzNjZ|ODFOwG0v NV7xdINbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFc5PjBpPkK3NVg4QDZyPD;hQi=>
Sf21 NGroO4RHfW6ldHnvckBie3OjeR?= NXHD[oM{OTBibXnudy=> M{LtU2lvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QufGGpZ3XkJJJm[2:vYnnuZY51KGi3bXHuJJdqdGRvdInw[UBHVFR|IDi1OlQhfG9iZX7kJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgd4YzOSClZXzsd{B2e2mwZzDBZox1cWSnIHHzJJN2[nO2cnH0[UBqdiCycnXz[Y5k\SCxZjDb[4FudWF|M2DdRXRRKGGodHXyJFExKG2rboOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDt[ZRpd2RuIFnDOVAhRSByLkCyOVUh|ryPLh?= Mnv6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyNEO3PVQoRjJ6MESzO|k1RC:jPh?=
Sf21 M33yU2Z2dmO2aX;uJIF{e2G7 NH;uXZUyOCCvaX7z NV\DTIUyUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hf2muZD30fZBmKE[OVEOgLFU3PCC2bzDlcoQhemW|aXT1[ZMqKER6M{XZJI12fGGwdDDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRic3[yNUBk\WyuczD1d4lv\yCDYnz0bYRmKGG|IIP1ZpN1emG2ZTDpckBxemW|ZX7j[UBw\iCdZ3HtcYE{O1CfQWTQJIFnfGW{IEGwJI1qdnNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yCvZYToc4QtKEmFNUCgQUAxNjJ|NUWg{txONg>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB2M{e5OEc,OjhyNEO3PVQ9N2F-
MV4-11 NXvhSppDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2XkVGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1XPC1zMTDj[YxteyCqYYLic5JqdmdiRlzUN{1KXERibYX0ZY51KGK7IGjUWEBie3OjeTygS2k2OCB;IEGuNVA{KM7:TT6= MlHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyNEO3PVQoRjJ6MESzO|k1RC:jPh?=
THP1 MnLiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDUTHAyKGOnbHzzJIhiemKxcnnu[{B4cWymLYT5dIUhTkyWMzDifUBZXFRiYYPzZZktKEeLNUCgQUAzNjF{NTFOwG0v MkSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyNEO3PVQoRjJ6MESzO|k1RC:jPh?=
BAF3 MkDSRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUK3NkBpenN? NH71O3hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFLBSlMh[2WubIOgbIFz[m:{aX7nJGZNXDNvSWTEMWQ5OzWJIH31eIF1cW:wIHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7LDDHTVUxKD1iMD6wNFA{KM7:TT6= MmfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MmTxO|IhcHK| NXq4d3QzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDCRWY{KGOnbHzzJIhiemKxcnnu[{BHVFR|LVnUSE1FQDN3QTDteZRifGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygS2k2OCB;IECuNFAxOyEQvF2u MmD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 MYHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MUW3NkBpenN? MlX1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEQV[zJINmdGy|IHjhdoJwemmwZzDGUHQ{NUR6M{XIJI12fGG2aX;uJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBIUTVyIE2gNE4xODB|IN88UU4> NVfNNoZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
MOLM13 MnSwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MoHtO|IhcHK| NXXweot3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNU2xOOTNiY3XscJMhcGG{Yn;ybY5oKE[OVEOtTXRFKG23dHH0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5yMECzJO69VS5? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
BAF3 Mn7QRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MYG3NkBpenN? NHv5OFVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFLBSlMh[2WubIOg[ZhxemW|c3nu[{BVTUxvZoXz[YQhTkyWMzDrbY5ie2ViYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFfJOVAhRSByLkCwNFMh|ryPLh?= NH[2fYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
BAF3 NGHPbIdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVG3NkBpenN? M3mwSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQlHGN{Bk\WyuczDoZZJjd3KrbnegSmxVOy2LVFStSFg{PU5ibYX0ZZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFfJOVAhRSByLkCwNFMh|ryPLh?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
MOLM14 NYW3WpFkSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mor4O|IhcHK| NIfUZVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3PUG0yPCClZXzsd{Bp[XKkb4LpcochTkyWMz3JWGQhdXW2YYTpc44h[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTldk1IdG9iYYPzZZktKEeLNUCgQUAxNjByMEOg{txONg>? M4PST|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
BAF3 MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MkPWO|IhcHK| M3OzW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQlHGN{Bk\WyuczDoZZJjd3KrbnegSmxVOy2LVFSgcZV1[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3HcI8h[XO|YYmsJGdKPTBiPTCwMlAxODNizszNMi=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
MV4-11 MWDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3e0S|czKGi{cx?= NH32OHZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhT0l3MDC9JFAvODByMzFOwG0v NIHEWGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
BAF3 NW[wU2pGSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NV\0To83PzJiaILz Mly5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDUSWwu\nW|ZXSgVGRITlKjbIDoZU11emGwc3\vdo1m\CCvb4Xz[UBDSUZ|IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5yMECzJO69VS5? NVnWNJNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
BAF3 MlHFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MVu3NkBpenN? NW[zNY9iSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDCRWY{KGOnbHzzJIhiemKxcnnu[{BHVFR|LVS4N|V[KG23dHH0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5yMEKg{txONg>? NH;IbmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
BAF3 M1zDOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 Ml3wO|IhcHK| NGPxVXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHRGVC2odYPl[EBRTEeIUnLleIEufHKjboPmc5Ju\WRibX;1d4UhSkGIMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUeubzDhd5NigSxiR1m1NEA:KDBwMEC0OEDPxE1w MoHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 NYPFNmFwSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mm\hO|IhcHK| M3;aUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQlHGN{Bk\WyuczDoZZJjd3KrbnegSmxVOy2GOEO1WkBufXSjdHnvckBi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhT0l3MDC9JFAvODFzIN88UU4> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
BAF3 M1PKOWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M4nNVVczKGi{cx?= M3nV[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQlHGN{Bk\WyuczDoZZJjd3KrbnegSmxVOy2LVFStSFg{PWSnbDDteZRifGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygS2k2OCB;IECuNFEzKM7:TT6= MorhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
Sf9 NWTxSVI1TnWwY4Tpc44h[XO|YYm= MVexJIhz M{D3W2lvcGmkaYTpc44hd2ZiaIXtZY4hVVZ2LUGxJINmdGy|LXTldol3\WRiSHnzMZRi\2enZDDGUHQ{KEmWRDDteZRidnRiKEW2OEB1dyB7OUOgdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDz[lkh[2WubIOgeZNqdmdicH;sfUApPDpzIFfseUwhXHm{KTDhd{B{fWK|dILheIUh[W[2ZYKgNUBpeiCkeTDBSHAuT2yxIHvpcoF{\SCjc4PhfUwhUUN3MDC9JFAvODF4ODFOwG0v M2jXZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
BAF3 NWP4UJBoSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFTq[Ik4OiCqcoO= NH;yZldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHRGVC2odYPl[EBkU0mWLYTyZY5{\m:{bXXkJI1wfXOnIFLBSlMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3HcI8h[XO|YYmsJGdKPTBiPTCwMlAyPyEQvF2u MoLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 NIixcG1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MX:3NkBpenN? MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IGTFUE1nfXOnZDDDV2YyWi22cnHud4Zwem2nZDDtc5V{\SCEQV[zJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7LDDHTVUxKD1iMD6wNlQh|ryPLh?= MoXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 M4XUWGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 Mo\PO|IhcHK| MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEKDRkOgZ4VtdHNiaHHyZo9zcW6pIF\MWFMuUVSGLVS4N|V[KG23dHH0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5yMkig{txONg>? M4rnSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
Sf9 M3PXOGZ2dmO2aX;uJIF{e2G7 MnXrNUBpeg>? M{P4TGlvcGmkaYTpc44hd2ZiaIXtZY4hUEx4MDDj[Yxtey2mZYLpeoVlKEircz30ZYdo\WRiRlzUN{ApPTZ2IITvJFk6OyC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKHOoOTDj[YxteyC3c3nu[{Bxd2y7IDi0PlEhT2y3LDDUfZIqKGG|IIP1ZpN1emG2ZTDh[pRmeiBzIHjyJIJ6KEGGUD3HcI8hc2mwYYPlJIF{e2G7LDDJR|UxKD1iMD6wN|A1KM7:TT6= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
BAF3 M1i5[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MWm3NkBpenN? M1zzWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQlHGN{Bk\WyuczDoZZJjd3KrbnegSmxVOy2LVFStSFg{PUlibYX0ZZRqd25iYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFfJOVAhRSByLkC1OkDPxE1w M3P5[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
BAF3 NH7m[G5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NIiwOVU4OiCqcoO= NGrDV|JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHRGVC2odYPl[EBFTFJ{LYTyZY5{\m:{bXXkJI1wfXOnIFLBSlMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3HcI8h[XO|YYmsJGdKPTBiPTCwMlEyKM7:TT6= NGDhXFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
BAF3 M4\hTGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHG5dnE4OiCqcoO= NVnPOHFbSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBVTUxvZoXz[YQhTESUMT30doFve2[xcn3l[EBud3W|ZTDCRWY{KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBIUTVyIE2gNE4yOiEQvF2u NWDQUohFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
CMK M2S1e2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NIC2fWs4OiCqcoO= NFXOemNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPNT{Bk\WyuczDlfJBz\XO|aX7nJGZNXDNia3nuZZNmKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5zMjFOwG0v Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
BAF3 NHnZPIxCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVy3NkBpenN? NGTaUplCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHRGVC2odYPl[EBTTVRvdILhcpNnd3KvZXSgcY92e2ViQlHGN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygS2k2OCB;IECuNVMh|ryPLh?= MlfzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
HL60 NHz5W5ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MYi3NkBpenN? MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiZYjwdoV{e2mwZzDGUHQ{KGurbnHz[UBi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhT0l3MDC9JFAvOTNizszNMi=> NEfmZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
BAF3 M3LQPGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MoHlO|IhcHK| M3fmXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgWGVNNW[3c3XkJGZNXDRvdILhcpNnd3KvZXSgcY92e2ViQlHGN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygS2k2OCB;IECuNlch|ryPLh?= NX72PVd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
BAF3 NI\lb4NCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MliwO|IhcHK| NUT2dG1jSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDCRWY{KGOnbHzzJIhiemKxcnnu[{BHVFR|LVu2OlNSKG23dHH0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCJSUWwJF0hOC5|NzFOwG0v M2rpNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
BAF3 MlPYRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmLOO|IhcHK| MnfYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDUSWwu\nW|ZXSgUGNMNXS{YX7z[o9zdWWmIH3veZNmKEKDRkOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFfJOVAhRSByLkSg{txONg>? M2i4clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
U937 MlXyRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXXld2x6PzJiaILz NULKOG5vSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIV5eHKnc4PpcochTkyWMzDrbY5ie2ViYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFfJOVAhRSBzLkKg{txONg>? NGjXOHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
SKM1 MULBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MU[3NkBpenN? MnfORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVS12xJINmdGy|IHX4dJJme3OrbnegSmxVOyCtaX7hd4Uh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTldk1IdG9iYYPzZZktKEeLNUCgQUAyNjNizszNMi=> NG\nVog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
NB4 MUnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnjLO|IhcHK| NU\aU3lnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDORlQh[2WubIOg[ZhxemW|c3nu[{BHVFR|IHvpcoF{\SCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUeubzDhd5NigSxiR1m1NEA:KDdwMzFOwG0v NX:ybW9VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
MOLM13 M3vUTmNmdGxiY4njcIUh[XO|YYm= MmPwNVAxKG6P MmT5NVIhcHK| NFfiVXhE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBOV0yPMUOgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IFewM2cyKHCqYYPlJIF1KDFyMDDuUUBi\nSncjCxNkBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\C2IQVPTJIFv[Wy7c3nz MlfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
MOLM14 NHH4[4VE\WyuIHP5Z4xmKGG|c3H5 Mn3JNVAxKG6P M1L4eVEzKGi{cx?= M{HXT2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJG1QVE1zNDDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRiR{CvS|EheGijc3WgZZQhOTByIH7NJIFnfGW{IEGyJIhzeyCkeTDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pIHLhd4VlNU[DQ2OgZY5idHm|aYO= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
MOLM13 NFWyeFZCeG:ydH;zbZMh[XO|YYm= MUKxNFAhdk1? NXe4ZVc6OTJiaILz MV\JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2RTF2xN{Bk\WyuczDhd5Nme3OnZDDhd{BkdGWjdnHn[UBw\iCSQWLQJIF1KDFyMDDuUUBi\nSncjCxNkBpenNiYomgbY1ufW6xYnzveJRqdmdibXX0bI9l NW\IOFBtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
MV4-11 NYTnUVY2SXCxcITvd4l{KGG|c3H5 M{WwUlExOCCwTR?= NYG0XVVpPDhiaILz NHyycJpKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIF3WOE0yOSClZXzsd{Bie3Onc4Pl[EBieyClbHXheoFo\SCxZjDQRXJRKGG2IEGwNEBvVSCjZoTldkA1QCCqcoOgZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m M{jKRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVI{Lz5{OEm1OlkzOzxxYU6=
MOLM13 NXLyR211SXCxcITvd4l{KGG|c3H5 M1PUflExOCCwTR?= MVGxNkBpenN? M4LYWWlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTV;MUVE{KGOnbHzzJIF{e2W|c3XkJIF{KGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDheEAyODBibl2gZYZ1\XJiMUKgbJJ{KGK7IHntcZVvd2Kub4T0bY5oKG2ndHjv[C=> NXTz[JpORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OjNpPkK4PVU3QTJ|PD;hQi=>
MOLM14 MmfPRZBweHSxc3nzJIF{e2G7 MlXjNVAxKG6P MV2yOEBpenN? MXzJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2RTF2xOEBk\WyuczDhd5Nme3OnZDDhd{BkdGWjdnHn[UBw\iCSQWLQJIF1KDFyMDDuUUBi\nSncjCyOEBpenNiYomgbY1ufW6xYnzveJRqdmdibXX0bI9l Ml36QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
MOLM14 M{C4XGFxd3C2b4Ppd{Bie3OjeR?= NXXDd|JSOTByIH7N MkfONlQhcHK| MVfJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2RTF2xOEBk\WyuczDhd5Nme3OnZDDhd{BkdGWjdnHn[UBw\iClYYPwZZNmNTNiYYSgNVAxKG6PIHHmeIVzKDJ2IHjyd{BjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3S= Mkm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NlMoRjJ6OUW2PVI{RC:jPh?=
MV4-11 NGXVU4VCeG:ydH;zbZMh[XO|YYm= NH:wfmwyODBibl2= NFPmTIw1QCCqcoO= M{\MWmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTW[0MVEyKGOnbHzzJIF{e2W|c3XkJIF{KGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDheEAyODBibl2gZYZ1\XJiNEigbJJ{KGK7IHntcZVvd2Kub4T0bY5oKG2ndHjv[C=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmyN{c,Ojh7NU[5NlM9N2F-
MV4-11 NWfLXoptS2WubDDjfYNt\SCjc4PhfS=> MUSxNFAhdk1? MVKxNkBpenN? MVzD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNWlQuOTFiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEdyL1exJJBp[XOnIHH0JFExOCCwTTDh[pRmeiBzMjDodpMh[nlicILvdIllcXWvIHnv[Ill\SC|dHHpcolv\yCkYYPl[E1HSUOVIHHuZYx6e2m| NF21[Gc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkzOyd-Mki5OVY6OjN:L3G+
Saos-2 Mn\YdWhVWyCjc4PhfS=> MnGzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= MkLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) MlnPdWhVWyCjc4PhfS=> Ml;NdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| MojzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 NIrWUW9yUFSVIHHzd4F6 M{e4UJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{ Mkf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 NUj5OnVIeUiWUzDhd5NigQ>? Ml:xdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUGFPNTViY3XscJM> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY NI\HNXlyUFSVIHHzd4F6 M3jmd5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGRCV1liY3XscJM> MnLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH NUniT5ZmeUiWUzDhd5NigQ>? MWnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNVOKIHPlcIx{ MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
A673 M2PoWZFJXFNiYYPzZZk> NEHTbXZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? M1zTU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 NWfjN2M{eUiWUzDhd5NigQ>? NG\yOJVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1|NzDj[Yxtew>? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
U-2 OS M{i2WpFJXFNiYYPzZZk> NHSxOZpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBWNTJiT2OgZ4VtdHN? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 MVnxTHRUKGG|c3H5 NVjLW5lKeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 NUnsT4tqeUiWUzDhd5NigQ>? M1\Se5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJM> NUjFV49JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 NHHKOJpyUFSVIHHzd4F6 MlrmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= NVPTT3dGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NVfxZWtPeUiWUzDhd5NigQ>? MWLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MlnIdWhVWyCjc4PhfS=> M4\wdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 NX;GeFVMeUiWUzDhd5NigQ>? M3PTNpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DOTZ2MzDj[Yxtew>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC NHXoVplyUFSVIHHzd4F6 M{KzVpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MX\xTHRUKGG|c3H5 MnH2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2ouT0KPMjDj[Yxtew>? NY\pXVVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 NVHRNYl3eUiWUzDhd5NigQ>? NVm1fYRHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? M1TFdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MOLM13 NY[5PIZLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEjWVG04OiCqcoO= Ml\4S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUW9NVTF|IHPlcIx{KGGodHXyJFczKGi{czDifUBk[WylZXnuJGFOKGS7ZT3iZZNm\CCobIXvdoV{[2WwY3WgZZN{[XluIFfJOVAhRSByLkCwNkDPxE1w Mn7YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
MV4-11 NEjzUmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1;qWVczKGi{cx?= NUHwVmE3T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVVZ2LUGxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSClYXzj[YlvKEGPIHT5[U1j[XOnZDDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGdKPTBiPTCwMlAxOyEQvF2u M4fRW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkeyNFQ6Lz5{OU[3NlA1QTxxYU6=
BA/F3 MWDGeY5kfGmxbjDhd5NigQ>? NWHOcZJDPzJiaILz NH7HcXlKdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCBqdX7rco94diCxcnnnbY4qKHS{YX7z[oVkfGWmIHnuJI1wfXOnIFLBM2Y{KGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBk[WylZXnuJGFOKGS7ZT3iZZNm\CCobIXvdoV{[2WwY3WgZZN{[XluIFfJOVAhRSByLkCwO{DPxE1w MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ5MkC0PUc,Ojl4N{KwOFk9N2F-
Sf9 MXjGeY5kfGmxbjDhd5NigQ>? Moq2TY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIFfTWE9JcXN4LX\1d4VlKHKnY3;tZolv[W62IHj1cYFvKE[OVEOgLHI2PzFidH:gV|k6OyC{ZYPp[JVmeyliSWTEJI12fGGwdDDlfJBz\XO|ZXSgbY4hW2Z7IHnud4VkfCClZXzsd{B2e2mwZzDwc4x6KCiDbHGsS4x2NEy7czzUfZIqKDZ8Mkq1PlEhcHmmcn;ido9ucWSnIHHzJJN2[nO2cnH0[UBqdiCycnXz[Y5k\SCxZjDb[4FudWFvM{PQYWFVWCxiSVO1NEA:KDBwMEGg{txONg>? M2LoRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkeyNFQ6Lz5{OU[3NlA1QTxxYU6=
Sf9 Mmf3SpVv[3Srb36gZZN{[Xl? NE\sNnBKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygS3NVN0irc{[t[pV{\WRicnXjc41jcW6jboSge4lt\CC2eYDlJIh2dWGwIF\MWFMhMFJ3N{GgeI8hWzl7MzDy[ZNq\HWnczmg[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMhfXOrbnegdI9tgSBqQXzhMGdtfSyOeYOsWJlzMSB4OkK6OVoyKGi7ZILvZpJwdWmmZTDhd{B{fWK|dILheIUhcW5icILld4Vv[2Vib3[gX4didW2jLUOzVH1CXFBuIFnDOVAhRSByLkCzOkDPxE1w NX\pNJR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2O|IxPDlpPkK5OlczODR7PD;hQi=>
Kasumi-1 NEDZVHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVe3NkBpenN? MX;Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDLZZN2dWlvMTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2GuY3XpckBCVSCmeXWtZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDHTVUxKD1iMD6wOlQh|ryPLh?= MljvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
Sf9 NVvt[mxoTnWwY4Tpc44h[XO|YYm= NV7ib4JvUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC:KaYO2MYZ2e2WmIILlZ49u[mmwYX70JIh2dWGwIF\MWFMhMFJ3N{GgeI8hWzl7MzDy[ZNq\HWnczmgSFg{PVlibYX0ZY51KGW6cILld5Nm\CCrbjDT[lkhcW6|ZXP0JINmdGy|IIXzbY5oKHCxbImgLGFt[SyJbIWsUJl{NFS7cjmgOlozQjV8MTDofYRzd2K{b33p[IUh[XNic4Xid5Rz[XSnIHnuJJBz\XOnbnPlJI9nKFupYX3tZU0{O1CfQWTQMEBKSzVyIE2gNE4yOzZizszNMi=> MnfLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
BA/F3 NGTINpVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkjuO|IhcHK| MnfNS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgRmEwTjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JINidGOnaX6gRW0h\HmnLXLhd4VlKG[udX;y[ZNk\W6lZTDhd5NigSxiR1m1NEA:KDFwNEWyJO69VS5? MoK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
MV4-11 Mn[0SpVv[3Srb36gZZN{[Xl? Mo\ONE4xOSC2bzCxNFAhdk1? M4r3TFEhcHJ? NYD0dYdYUW6qaXLpeIlwdiCxZjDGUHQ{KEmWRDDteZRidnRiaX6gbJVu[W5iTW[0MVEyKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIFHLWEBxcG:|cHjvdplt[XSrb36gZZQhWzR5MzDheEAxNjBzIITvJFExOCCwTTDh[pRmeiBzIHjyJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN? M{DiO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkeyNFQ6Lz5{OU[3NlA1QTxxYU6=
MV4-11 NVLOcXN5TnWwY4Tpc44h[XO|YYm= M1PEelAvODFidH:gNVAxKG6P MYqxJIhz MV3Jcohq[mm2aX;uJI9nKE[OVEOgTXRFKG23dHHueEBqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hVUWNMT:yJJBpd3OyaH;yfYxifGmxbjDheEBUOjF5L{KyNUBifCByLkCxJJRwKDFyMDDuUUBi\nSncjCxJIhzKGK7IHntcZVvd2Kub4SgZY5idHm|aYO= M123b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkeyNFQ6Lz5{OU[3NlA1QTxxYU6=
MV4-11 M{P3[mZ2dmO2aX;uJIF{e2G7 MXuxJI5O NXLWOoI4OSCqch?= NHvUdWdKdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCCjdYTvdIhwe3Cqb4L5cIF1cW:wIHH0JHk2QDlxNUmxJIlvKGi3bXHuJG1XPC1zMTDj[YxteyCjdDCxJI5OKGGodHXyJFEhcHJiYomgbY1ufW6xYnzveEBidmGueYPpdy=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ5MkC0PUc,Ojl4N{KwOFk9N2F-
MV4-11 MYnGeY5kfGmxbjDhd5NigQ>? M2PGbVEhdk1? MkPFNUBpeg>? MnrtTY5pcWKrdHnvckBw\iCITGSzJGlVTCCvdYThcpQh[XW2b4Doc5NxcG:{eXzheIlwdiCjdDDZPFQzKGmwIHj1cYFvKE2YND2xNUBk\WyuczDheEAyKG6PIHHmeIVzKDFiaIKgZpkhcW2vdX7vZoxwfCCjbnHsfZNqew>? M17zWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkeyNFQ6Lz5{OU[3NlA1QTxxYU6=
MV4-11 NHu5RZlHfW6ldHnvckBie3OjeR?= NITySmIxNjBzIITvJFExOCCwTR?= MnG3NUBpeg>? NIXpcHpKdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCCrbjDoeY1idiCPVkStNVEh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iU2TBWFUheGixc4Doc5J6dGG2aX;uJIF1KFl4OUSgZZQhOC5yMTD0c{AyODBibl2gZYZ1\XJiMTDodkBjgSCrbX31co9jdG:2IHHuZYx6e2m| MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ5MkC0PUc,Ojl4N{KwOFk9N2F-
MV4-11 MUfGeY5kfGmxbjDhd5NigQ>? MlfMNE4xOSC2bzCxNFAhdk1? MWWxJIhz MW\Jcohq[mm2aX;uJI9nKE[OVEOgTXRFKG23dHHueEBqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hTVKNMT:yJJBpd3OyaH;yfYxifGmxbjDheEBVOjB{L2myNFQh[XRiMD6wNUB1dyBzMECgcm0h[W[2ZYKgNUBpeiCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz MnPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
MV4-11 NYf3OWtFTnWwY4Tpc44h[XO|YYm= NXvJbY1zOTBibXevb4c> MYCyOEBpenN? NVzv[Y5LUW5idnn2c{BqdmirYnn0bY9vKG:oIF\MWFMhUVSGIH31eIFvfCCrbjDoeY1idiCPVkStNVEh[2WubIOgfIVvd2e{YX\0[YQhcW5iYYTofY1q[yCwdT;ueUBud3W|ZTDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCVVFHUOUBxcG:|cHjvdplt[XSrb36gZZQhYTZ7NDDheEAyOCCvZz;r[{whcXBiYX\0[ZIhOjRiaILzJIJ6KGmvbYXuc4Jtd3RibXX0bI9lKHKnbHH0bZZmKHSxIHPvcpRzd2x? MoD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4N{KwOFkoRjJ7NkeyNFQ6RC:jPh?=
MV4-11 M2LkO2Z2dmO2aX;uJIF{e2G7 MkPFNE4yKHWP M17SPFQhcHK| M{T5VGlvcGmkaYTpc44hd2ZiRlzUN{BqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEGNVDDwbI9{eGixconsZZRqd25iYYSgV4VzPDd|IILld4llfWViYYSgNE4yKHWPIHHmeIVzKDRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh7NEm0OEc,Ojl6OUS5OFQ9N2F-
MV4-11 Moe1SpVv[3Srb36gZZN{[Xl? M2G5eVAvOSC3TR?= NHzxOJg1KGi{cx?= MWDJcohq[mm2aX;uJI9nKE[OVEOgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBUXEGWNTDwbI9{eGixconsZZRqd25iYYSgWJlzPjl2IILld4llfWViYYSgNE4yKHWPIHHmeIVzKDRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= M4iwWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEm0PVQ1Lz5{OUi5OFk1PDxxYU6=
MV4-11 M2XNXGZ2dmO2aX;uJIF{e2G7 MnnWNE4yKHWP NXjVOnhyPCCqcoO= MUjJcohq[mm2aX;uJI9nKE[OVEOgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBGWktzL{KgdIhwe3Cqb4L5cIF1cW:wIHH0JHRpejJyMj;UfZIzODRicnXzbYR2\XNiYYSgNE4yKHWPIHHmeIVzKDRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= M3HFWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEm0PVQ1Lz5{OUi5OFk1PDxxYU6=
MV4-11 MVXGeY5kfGmxbjDhd5NigQ>? M3v2eVAvOSC3TR?= M1fq[VQhcHK| NG\vdmZKdmirYnn0bY9vKG:oIF\MWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckW4PU82QTFicnXzbYR2\XNiaX6gbJVu[W5iTW[0MVEyKGOnbHzzJIF1KDBwMTD1UUBi\nSncjC0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NG\LNHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi5OFk1PCd-Mkm4PVQ6PDR:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
phospho-FLT3 / FLT3; 

PubMed: 22875611     


(B) The same cells were also harvested for Western blot analysis after treatment for 90 min with different concentrations of AC220. The phosphorylation of the different FLT3 proteins was determined using a phospho-FLT3 antibody.

p-STAT5 / STAT5 / β-catenin / p-AKT / AKT / p-ERK / ERK / p-S6 / S6; 

PubMed: 28625976     


Western blot analysis of AC220 treated MV4-11 cells. Cells were starved for overnight and treated with AC220 at indicated concentrations. Cells were harvested after 2 hours treatment and lysed. Then western blots analysis was performed. The quantification of bands are shown below the gel.

22875611 28625976
Immunofluorescence
WGA / FLT3 ; 

PubMed: 28895560     


Immunofluorescence staining of FLT3-WT, FLT3 mutants or empty vector with or without AC220 treatment in transiently transfected U2OS cells. WGA (wheat germ agglutinin). Scale bar: 25 μm.

28895560
Growth inhibition assay
Cell viability; 

PubMed: 23967177     


K562/ABCB1 and K562/ABCG2 cells exhibit collateral sensitivity toquizartinib. K562, K562/ABCB1 and K562/ABCG2 cells were plated in the presence of quizartinib in increasing concentrations for 96 hours and viable cells were measured using the WST-1 assay.

23967177
体内研究 AC220作用于移植瘤模型,具有好的药品属性,极高的药效和耐受性。在体内,AC220作用于FLT3-ITD AML鼠模型,抑制FLT3活性,明显延长寿命;作用于依赖FLT3的移植瘤鼠模型时,根除肿瘤;作用于病人原代细胞时,有效抑制FLT3活性。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

激酶实验:

为了测定AC220抑制无靶点激酶的效果,为了比较AC220和其他FLT抑制剂的选择性,使AC220作用于KinomeScan一组402种激酶进行结合试验,其中80%为典型的人类蛋白激酶。测定所有激酶的解离常数。计算绝对选择性和作用于FLT的相对选择性。
细胞实验:[1]
- 合并
  • Cell lines: MV4-11和RS4;11细胞
  • Concentrations: 0-100 nM
  • Incubation Time: 72小时,及2小时
  • Method: 在增殖实验中,细胞培养在低血清培养基(含0.5% FBS)中过夜, 然后按每孔4×104个细胞接种在96孔板上,然后加入AC220,37oC下温育72小时。测定细胞活力。测定抑制FLT3自磷酸化的效果, 细胞培养在低血清培养基(含0.5% FBS)过夜,然后按每孔4×105个细胞接种在96孔板上。细胞和AC220一起在37oC下温育2小时,诱导 RS4;11细胞中FLT3自磷酸化, 加入100 ng/mL FLT3配位体,持续15分钟。转移细胞溶解物到用FLT3抗体包被的96孔板上。然后加入作用于FLT3的生物素化抗体,测定全部FLT3,或者作用于磷酸酪氨酸的抗体,测定FLT3自磷酸化。使用 SULFO标记的链霉亲和素二抗进行电化学发光测定。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 雌性裸鼠或严重联合免疫缺陷鼠
  • Dosages: 10 mL/kg
  • Administration: 口服饲喂处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 33 mg/mL (58.85 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
15% Captisol
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 560.67
化学式

C29H32N6O4S

CAS号 950769-58-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04459598 Completed Drug: Efavirenz|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 8 2020 Early Phase 1
NCT04473664 Completed Drug: Quizartinib Hepatic Impairment|Moderate Impaired Hepatic Function Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. September 29 2020 Phase 1
NCT04459585 Completed Drug: Dabigatran Etexilate Mesylate|Drug: Quizartinib Healthy Subjects|Drug-drug Interaction|Pharmacokinetics|Quizartinib Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. August 28 2020 Early Phase 1
NCT04209725 Terminated Drug: CPX-351|Drug: Quizartinib Leukemia Myeloid Acute SCRI Development Innovations LLC June 3 2020 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is it possible to alter the captisol concentration to make it more dissolvable i.e 20% or 25% captisol ?

  • 回答:

    In 15% Captisol, the compound forms s suspension at 30mg/ml. Increasing the percentage of Captisol will not convert the mixture into solution. You can use suspension for oral gavage feeding.

Target Protein Ligand Inhibitors with Unique Features

Tags: 购买Quizartinib (AC220) | Quizartinib (AC220)供应商 | 采购Quizartinib (AC220) | Quizartinib (AC220)价格 | Quizartinib (AC220)生产 | 订购Quizartinib (AC220) | Quizartinib (AC220)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID