Estradiol

For research use only. Not for use in humans.

目录号:S1709 别名: 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol 中文名称:雌二醇

Estradiol Chemical Structure

CAS No. 50-28-2

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol)是一种人类性激素和类固醇,也是主要的女性性激素。Estradiol 可通过 estrogen receptor β (ERβ) 通路来上调 IL-6 的表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2202.75 现货
RMB 780.47 现货
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客户使用Selleck生产的Estradiol发表文献11篇:

产品安全说明书

Estrogen/progestogen Receptor抑制剂选择性比较

生物活性

产品描述 Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol)是一种人类性激素和类固醇,也是主要的女性性激素。Estradiol 可通过 estrogen receptor β (ERβ) 通路来上调 IL-6 的表达。
靶点
Estrogen receptor [1]
体外研究

Estradiol诱导海马CA1锥体细胞产生新的树突棘和突触。Estradiol治疗导致NMDA受体增加46%结合力。Estradiol治疗增加NMDA受体结合以及相应的树突棘和突触密度。Estradiol处理导致CA1区锥体细胞对NMDA受体介导的突触输入的敏感性增加,而这种增加与这些细胞的顶树突树中Estradiol诱导树突棘密度的增加有很好的相关性。[1] 在急性分离和培养的纹状体神经元中,17 beta-estradiol可逆地通过钡离子通道减少钙离子进入。 17 alpha-estradiol也减少了钡离子电流,但是显著比17 beta-estradiol对大鼠纹状体神经元的效果较差。[2] 17 beta-estradiol剂量依赖性抑制IL-1,TNF-α和IL-1和TNF-α诱导的IL-6的产生。Estradiol抑制新生小鼠颅盖骨来源的原骨细胞培养物中TNF-α诱导的IL-6的产生和破骨细胞的生成。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 MlPUSpVv[3Srb36gZZN{[Xl? NWrTXnJ6TGm|cHzhZ4Vu\W62IH;mJHs{UF2nc4TyZYRqd2xiZoLvcUBGe3S{b3flckBz\WOncITvdkBqdiCPQ1[tO{Bk\WyuczygTWM2OD1yLkCwN:69VQ>? M4rJS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEi2PFMoRjF3NEi2PFM9N2F-
MCF-7 M{myb2Z2dmO2aX;uJIF{e2G7 NVXaZXZ4TW[oZXP0bZZmKGSxc3Wg[o9zKFt|SG2tJIV{fHKjZHnvcEBi\2GrboP0JJBzd2yrZnXyZZRqd25ib3[gUWNHNTdiY3XscJMtKEWFNUC9NE4xODByMt88US=> MkTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OU[4NUc,Ojd4OU[4NVww[T5?
Ishikawa cells MlWxSpVv[3Srb36gZZN{[Xl? MV\Dc45k\W62cnH0bY9vKG:oIHPvcZBwfW6mIILldZVqemWmIITvJIlv\HWlZTC1NEAmKG:oIITo[UBu[XirbYXtJJN1cW23bHH0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIlvKEm|aHnrZZdiKGOnbHzzMEBGSzVyPUCuNFAxODIQvF2= M4\1ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh{MEG1PFcoRjh{MEG1PFc9N2F-
MCF-7 M1\RTGZ2dmO2aX;uJIF{e2G7 M1W1Rmlv\HWldHnvckBw\iCyU{KgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIF3DSk04KG2jbX3hdpkh[2G{Y3nuc41iKGOnbHzzMEBGSzVyPUCuNFAxODl7Nt88US=> MnfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDZ{N{[xOUc,QDZ{N{[xOVww[T5?
MCF-7 M1jWfWZ2dmO2aX;uJIF{e2G7 MWfJcoR2[3Srb36gc4YheFN{IH3SUmEh\XiycnXzd4lwdiCrbjDoeY1idiCPQ1[tO{Bu[W2vYYL5JINiemOrbn;tZUBk\WyuczygSWM2OD1yLkCwNFI6Os7:TR?= M{nlflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh4Mke2NVUoRjh4Mke2NVU9N2F-
mammary cancer cells NWDwVIY{TnWwY4Tpc44h[XO|YYm= MkfSRYZncW6rdImg[ZN1em:pZX6gdoVk\XC2b4Kgc4YhVUOILUegbJVu[W5ibXHtcYFzgSClYX7j[ZIh[2WubIOsJGVEPTB;MD6wNFM5|ryP NFj2VFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NkK3OlE2Lz56NkK3OlE2RC:jPh?=
HeLa NF7tfnBHfW6ldHnvckBie3OjeR?= MYjUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBGOjF5YnX0ZWchfXC2YXvlJIlvKE:jdICxMYV5eHKnc4PpcochUGWOYTDj[YxteyxiS3m9OE44|ryP MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85Pzd7OEm0K|45Pzd7OEm0QE9iRg>?
MCF-7 MljkSpVv[3Srb36gZZN{[Xl? NI\mbGdIem:5dHigdoV{eG:wc3WgbY4h[3WudIXy[ZMhd2ZiTVPGMVchMGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscEBtcW6nKTDj[YxteyxiRVO1NF0xNjByMECwNVXPxE1? NX\WSodwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUO1O|U{Oyd-OUO1O|U{OzxxYU6=
MCF-7 MnLqSpVv[3Srb36gZZN{[Xl? NXPuTYxNUW6mdXP0bY9vKG:oIIDTNkBI\W6nIHX4dJJme3Orb36gbY4hcHWvYX6gUWNHNTdiY3XscJMtKEWFNUC9NE4xODByM{Cx{txO MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86OzdzMkSxK|46OzdzMkSxQE9iRg>?
MCF-7 MV\GeY5kfGmxbjDhd5NigQ>? NIDsOlVC\m[rbnn0fUBnd3JiZYP0do9o\W5icnXj[ZB1d3Jib3[gbJVu[W5iTVPGMVch[2WubIOsJGVEPTB;MD6wNFAyQM7:TR?= M3G0cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl|N{GyOFEoRjl|N{GyOFE9N2F-
HRPE NXXseHY2TnWwY4Tpc44h[XO|YYm= MlfhWHBgXFKDTmPQU3JVTVJ8IHnubIljcXSrb36gc4YhXEWDIIXweIFs\SBqVFXBPkAzOCC3TTmgbY4hV0OWMz3lfJBz\XO|aX7nJGhTWEViY3XscJMtKEmFNUC9NU4y|ryP M2DM[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6M{CwNlIoRjl6M{CwNlI9N2F-
MCF-7 M{TXWmZ2dmO2aX;uJIF{e2G7 M1;FSGFod26rc4Sg[YZn\WO2IH;uJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJIlvKE2FRj23JINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZYxxcGFuIFXDOVA:OC5yMECwNFfPxE1? M{nhcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkezNFk6Lz5zME[3N|A6QTxxYU6=
HeLa M1jrS2Z2dmO2aX;uJIF{e2G7 MkK4RYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGuMEBGSzVyPUCuNFAxODMQvF2= M37CRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOUC2NlgxLz5zMUmwOlI5ODxxYU6=
HeLa MnTOSpVv[3Srb36gZZN{[Xl? NWrrNWJzSWO2aY\heIlwdiCxZjDld5Rzd2enbjDy[ZNxd26|ZTDlcIVu\W62IHnuJGhmVGFiY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iRYP0do9o\W5icnXj[ZB1d3JiYnX0ZU4tKEWFNUC9NE4xODByN988US=> M3;nPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOUC2NlgxLz5zMUmwOlI5ODxxYU6=
SH-SY5Y MnP0SpVv[3Srb36gZZN{[Xl? NUjoVpJmSWexbnnzeEBi[3Srdnn0fUBqdiC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjc4PhfUBqdiCVSD3TXVV[KG6ndYLvZoxie3SxbXGgZ4VtdHNiZYjwdoV{e2mwZzDFd5Rzd2enbjDy[YNmeHSxcjDi[ZRiNCCHQ{WwQVAvODByMd88US=> NV7w[pZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
HEK293 MVzGeY5kfGmxbjDhd5NigQ>? MYjUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBOWFBtIIXweIFs\SBqTWDQL|ohOC5{NTD1UUkhcW5iT1PUN{1mgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczygTWM2OD1{Lki4{txO MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjFzME[wO{c,OTJzMUC2NFc9N2F-
HEK293 NX;s[lBJTnWwY4Tpc44h[XO|YYm= MV3UVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBOWFBtIIXweIFs\SBqTWDQL|ohOC5{NTD1UUkhcW5iT1PUNU1mgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczygTWM2OD13Lkez{txO NF3i[2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGxNFYxPyd-MUKxNVA3ODd:L3G+
MCF-7-2a MXXGeY5kfGmxbjDhd5NigQ>? NGXYdZlC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG|c3H5JIlvKE2FRj23MVJiKGOnbHzzJINwdXCjcnXkJJRwKGW|dILh[IlwdCCHMjygSWM2OD1yLkCwNFQ6|ryP NXnVPYV1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxN|k1PDdpPkGyNVM6PDR5PD;hQi=>
T47D NW\1RlJFTnWwY4Tpc44h[XO|YYm= NVTMco9tSWexbnnzeEBm\m[nY4Sgc44hfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hcW5iVES3SEBk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijLDDFR|UxRTBwMECwNFfPxE1? Mk\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2NUmwNVcoRjF{NEW5NFE4RC:jPh?=
T47D NHrTPJVHfW6ldHnvckBie3OjeR?= NGfReI9C\2:waYP0JIVn\mWldDDvckB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCWNEfEJINmdGy|IHX4dJJme3OrbnegbJVu[W5iZYP0do9o\W5icnXj[ZB1d3JiYnX0ZUwhTUN3ME2wMlAxODkQvF2= Mlu4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2NUmwNVcoRjF{NEW5NFE4RC:jPh?=
MCF-7 NFzHV|dHfW6ldHnvckBie3OjeR?= NW\1T2U6WGW{Y3XueEBi\2:waYP0bYMh[WO2aY\peJkh\m:{IHXzeJJw\2WwLXnu[JVk\WRicGOyJIV5eHKnc4Ppc44hcW5iTVPGMVch[2WubIOsJGVEPTB;MD6wNFAxOc7:TR?= NY\6dHpPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3OFk5QThpPkGyO|Q6QDl6PD;hQi=>
MCF-7 NF7td3BHfW6ldHnvckBie3OjeR?= MmnHSpVv[3Srb37hcEBGWmGucHjhJJJm[2WydH;yJIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG|c3H5JIlvKE2FRj23JINmdGy|LDDFSFUxRTBwMECwNFA1Ps7:TR?= NFLpbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkiyOFA1Oyd-MUK4NlQxPDN:L3G+
SAOS-2 M1n3fmZ2dmO2aX;uJIF{e2G7 MoDhSpVv[3Srb37hcEBGe3S{b3flckBz\WOncITvdkBj\XSjIHHjeIl3cXS7IHL5JI1mfGGubH;0bIlwdmWrbjDpcoR2[3Srb36gbY4hW0GRUz2yJINmdGy|LDDFSFUxRTBwMEC0O:69VQ>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NEC0N{c,OTJ6MkSwOFM9N2F-
MCF-7 breast tumor MVfGeY5kfGmxbjDhd5NigQ>? NFHCdGlG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IF3DSk04KGK{ZXHzeEB1fW2xcjDj[YxteyC3c3nu[{BOS0ZvNzDhd5NigS5uIFXDOVA:OC5yMECwNe69VQ>? NF3hb2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkiyOVk{PSd-MUK4NlU6OzV:L3G+
MCF-7-2a MUfGeY5kfGmxbjDhd5NigQ>? M17ZZWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZYN1cX[jdHWgcJVkcW[ncnHz[UBmgHC{ZYPzbY9vKGmwIF3DSk04NTKjIHPlcIx{NCCHQ{WwQVAvODByMElOwG0> M1faXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2N{[xNVk{Lz5zNEe2NVE6OzxxYU6=
Ishikawa Var-1 cells M3f3NGZ2dmO2aX;uJIF{e2G7 NH3ud4k4OiCqcoO= MnjXSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SCYYYKtNUBk\WyuczDhd5Nme3OnZDDhd{B{fGmvdXzheIlwdiCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBu\WG|dYLl[EBjgSCvZYThZo9tcXOvIH;mJJAudmm2cn;wbIVvd2xicHjvd5Bp[XSjc3WgZYZ1\XJiN{KgbJJ{NCCHQ{WwQVAvODByMES3{txO NVe1dZVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1Olg4PzBpPkG1OVY5PzdyPD;hQi=>
HeLa MY\GeY5kfGmxbjDhd5NigQ>? NGTRNIhKdmirYnn0bY9vKG:oIGuzTH1me3S{YXTpc4wh[mmwZHnu[{B1dyCqdX3hckBme3S{b3flckBz\WOncITvdkBj\XSjIHX4dJJme3OnZDDpckBJ\UyjIHPlcIx{NCCLQ{WwQVAvODJ2zszN NXq0ZnUxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW2OVg5PTFpPkG1OlU5QDVzPD;hQi=>
HeLa MUfGeY5kfGmxbjDhd5NigQ>? M4PFTWlvcGmkaYTpc44hd2ZiW{PIYYV{fHKjZHnvcEBjcW6maX7nJJRwKGi3bXHuJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijIHX4dJJme3OnZDDpckBJ\UyjIHPlcIx{NCCLQ{WwQVAvODJ6zszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi1NUc,OTV4NUi4OVE9N2F-
COS-1 NHrIT4dHfW6ldHnvckBie3OjeR?= NWfie3Y2UW5idnn0do8h[WexbnnzeEBi[3Srdnn0fUBnd3JiZYP0do9o\W5icnXj[ZB1d3JiYXzwbIEh\XiycnXzd4VlKGmwIFPPV{0yKGOnbHzzMEBGSzVyPUCuNFAzPM7:TR?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzM{SwO{c,OTV5MUO0NFc9N2F-
COS-1 NVXrSXY5TnWwY4Tpc44h[XO|YYm= NFjqfFFKdiC4aYTyc{Bi\2:waYP0JIFkfGm4aYT5JIZweiCnc4Tyc4dmdiC{ZXPldJRweiCkZYThJIV5eHKnc4Pl[EBqdiCFT2OtNUBk\WyuczygSWM2OD1yLkCwPVfPxE1? NHvJUnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexN|QxPyd-MUW3NVM1ODd:L3G+
293T NH7B[I9HfW6ldHnvckBie3OjeR?= M{nnfWRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xODB5Nd88US=> MlPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{MUm0OlMoRjF4MkG5OFY{RC:jPh?=
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HEK MYrGeY5kfGmxbjDhd5NigQ>? MXexPEBpenN? MUjB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGg[ZhxemW|c3XkJIlvKEiHSzDj[YxteyClbz3lfJBz\XO|aX7nJIJmfGFvbHHjeIFu[XOnIHHmeIVzKDF6IHjyd{BjgSCkZYThMYxi[3SjbXHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMES2{txO MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh6NUK3PUc,OjF6OEWyO|k9N2F-
UAS M{i4WGZ2dmO2aX;uJIF{e2G7 NX7hVWRJOThiaILz MVHB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDlfJBz\XO|ZXSgbY4hXUGVIHPlcIx{KGOxLXX4dJJme3OrbnegZoV1[S2uYXP0ZY1ie2ViYX\0[ZIhOThiaILzJIJ6KGKndHGtcIFkfGGvYYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODB{Nd88US=> NE\kNGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi4OVI4QSd-MkG4PFUzPzl:L3G+
HEC-1 NFPo[IJHfW6ldHnvckBie3OjeR?= NGPwTZozPCCqcoO= M17ZUmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDlfJBz\XO|ZXSgbY4hUEWFLUGgZ4VtdHNiY3;lfJBz\XO|aX7nJIJmfGFvZ3HsJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECxNlPPxE1? NUDobXBmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ|PD;hQi=>
U2OS M{TXemZ2dmO2aX;uJIF{e2G7 MXmyOEBpenN? M13xRmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDlfJBz\XO|ZXSgbY4hXTKRUzDj[YxteyClb3X4dJJme3OrbnegdGNOYC2qU2LDN{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHHmeIVzKDJ2IHjyd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNVXPxE1? NUHCXZFlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ|PD;hQi=>
HEC-1 NX[wbFIxTnWwY4Tpc44h[XO|YYm= M1TyVlI1KGi{cx?= NYTFOVdJSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGg[ZhxemW|c3XkJIlvKEiHQz2xJINmdGy|IHPv[ZhxemW|c3nu[{Bj\XSjLXfhcEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHHmeIVzKDJ2IHjyd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNu69VQ>? MmrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
U2OS MX\GeY5kfGmxbjDhd5NigQ>? Mn\xNlQhcHK| MmrJRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJHUzV1NiY3XscJMh[2:neIDy[ZN{cW6pIIDDUXgucFOUQ{OgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMEVOwG0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
BL21 MlvpSpVv[3Srb36gZZN{[Xl? M2TlNVEhcHJ? NIHpbVhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJG4uUGm|Nj30ZYdo\WRidHXyZol2dS2uYXLlcIxm\CCQUlnEMXNTSzNib3[gSXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBk\WyuczDh[pRmeiBzIHjyJIJ6KFSULV\SSXQh[XO|YYmsJGVEPTB;MD6wNFA2Ps7:TR?= NVrOZohzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ|PD;hQi=>
BL21 MXfGeY5kfGmxbjDhd5NigQ>? NELYe4MyKGi{ NF3DVmxC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJG4uUGm|Nj30ZYdo\WRidHXyZol2dS2uYXLlcIxm\CCQUlnEMXNTSzNib3[gSXJj\XSjIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy|IHHmeIVzKDFiaIKgZpkhXFJvRmLFWEBie3OjeTygSWM2OD1yLkCwNFnPxE1? M{TMOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
BL21 M4T3dmZ2dmO2aX;uJIF{e2G7 NX76cpY3OSCqch?= MlfpRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDOMWhqezZvdHHn[4VlKHSncnLpeY0udGGkZXzs[YQhTVKkZYThJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKGOnbHzzJIF{e2W|c3XkJIF{KHKnY4L1bZRu\W62IH;mJIZtfW:{ZYPj[YlvNWyjYnXscIVlKFOUQ{OgZYZ1\XJiMTDodkBjgSCWUj3GVmVVKGG|c3H5MEBGSzVyPUCuNFAyPc7:TR?= NHXqT4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGyNlU3Oyd-MkKxNlI2PjN:L3G+
BL21 NWiwW|A{TnWwY4Tpc44h[XO|YYm= NXXReoZFOSCqch?= M1jEeGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKEWUYXzwbIEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFiY3XscJMh[XO|ZYPz[YQh[XNicnXjdpVqfG2nboSgc4Yh\my3b4Lld4NmcW5vbHHi[Yxt\WRiU2LDN{Bi\nSncjCxJIhzKGK7IGTSMWZTTVRiYYPzZZktKEWFNUC9NE4xODQQvF2= MmDJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
Ishikawa cells NVzveINoTnWwY4Tpc44h[XO|YYm= NXO5bG5QOyCmYYnz NYi1dW1HSWexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBt\X[nbDDh[pRmeiB|IHThfZMhfXOrbnegVE1vcXS{b4Do[Y56dCCyaH;zdIhifGViYYOgd5Vje3S{YYTlMEBGSzVyPUCuNFAxQc7:TR?= M{G4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUe4OVUzLz5{MkG3PFU2OjxxYU6=
HepG2 M2fxcWZ2dmO2aX;uJIF{e2G7 MXKyOEBpenN? NVTBcXFTSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCKZYDHNkBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFAzOs7:TR?= M1LRb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN|I5Lz5{MkK4N|MzQDxxYU6=
HepG2 NEnoOWRHfW6ldHnvckBie3OjeR?= MWWyOEBpenN? NXXleJpkSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGg[ZhxemW|c3XkJIlvKEincFeyJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNVHPxE1? NH\TU5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK4N|MzQCd-MkKyPFM{Ojh:L3G+
MCF7 MmH6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NIPUNHY4OiCqcoO= NE\HUGdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuMYRm\mmlaXXueEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6wNFAxODZ2zszN MoDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkS2PFEoRjJ{NE[0OlgyRC:jPh?=
MCF7:D5L NEX6SnhHfW6ldHnvckBie3OjeR?= NIDUO3oyPiCqcoO= MYDFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KGW|dILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKE2FRke6SFVNKGOnbHzzJINwNWW6cILld5NqdmdiRWLFJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDseYNq\mW{YYPlJIdmdmViZYjwdoV{e2mxbjDh[pRmeiBzNjDodpMh[nlibXnjdo9xdGG2ZTDy[YFl\XJuIFXDOVA:OC5yMECwNVk3|ryP NISzOXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2OFY5OSd-MkK0OlQ3QDF:L3G+
Ishikawa cells NV3CZpo4TnWwY4Tpc44h[XO|YYm= NGe2VlA4OiCqcoO= M{HQcWV{fHKxZ3XubYMh[WO2aY\peJkh[XRiZYP0do9o\W5icnXj[ZB1d3JiaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGW6cILld5Nqd25iYX\0[ZIhPzJiaILzJIJ6KG2rY4LvdIxifGVicnXh[IVzNCCHQ{WwQVAvODByMEK0Ou69VQ>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4NE[4NUc,OjJ2NkS2PFE9N2F-
Ishikawa cells NXj5bVl7TnWwY4Tpc44h[XO|YYm= MVmwMlEhdk1? NEXNd|k4OiCqcoO= MkPJSYZn\WO2IH;uJINmdGxicILvcIln\XKjdHnvckBw\iCqdX3hckBKe2ira3H3ZUBk\WyuczDheEAxNjFibl2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?= NEL4NYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2OFY5OSd-MkK0OlQ3QDF:L3G+
CHO-K1 M{DVPWZ2dmO2aX;uJIF{e2G7 NILHVWYzPCCqcoO= M3H0XnRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKEWUYnX0ZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPMWsyKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODByM{dOwG0> MlnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
CHO-K1 MkS5SpVv[3Srb36gZZN{[Xl? NIXKRYYzPCCqcoO= NWLTfZRzXHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hd2ZiRWLhcJBp[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOe69VQ>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109 MlmxSpVv[3Srb36gZZN{[Xl? NWTjVmxbOjRiaILz NVrBbmJMSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKHKnY3XweI9zKEyERDDlfJBz\XO|ZXSgbY4hgWWjc4SgRWgyODliY3XscJMh[XO|ZYPz[YQh[XNiY3;tdI92dmRiY3;uZ4VvfHKjdHnvckBifCC5aHnjbEAyOCViYXfvcol{fCCjY4Tpeol1gSCxZjD0bIUhdWG6aX31cUBi[3Srdnn0fUBw\iCHMjDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiQXP0bZZqfHl;MD6wNFAy|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109 NVz4NlJWTnWwY4Tpc44h[XO|YYm= NEfPVIEzPCCqcoO= MYTB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDy[YNmeHSxcjDMRmQh\XiycnXzd4VlKGmwIInlZZN1KEGKMUC5JINmdGy|IHHzd4V{e2WmIHHzJINwdXCxdX7kJINwdmOnboTyZZRqd25iYYSge4hq[2hiMUClJIFod26rc4SgZYN1cX[rdImgc4YhfGinIH3hfIlufW1iYXP0bZZqfHlib3[gSVIh[W[2ZYKgNlQhcHK|IHL5JIFteGijLXfhcIFkfG:|aXThd4Uh[XO|YYmsJGFkfGm4aYT5QVAvODByMUlOwG0> M4\3UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O|IyPzxxYU6=
yeast AH109 MXrGeY5kfGmxbjDhd5NigQ>? NX3GSZc2OjRiaILz MoPCRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCHQ{WwQVAvODBzMEZOwG0> M3rBU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O|IyPzxxYU6=
yeast AH109 NHTxUHRHfW6ldHnvckBie3OjeR?= MVyyOEBpenN? NGHRV3pC\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W|c3XkJIF{KGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiRVO1NF0xNjByMUC4{txO MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
U2-OS MUfGeY5kfGmxbjDhd5NigQ>? NVuzV|U3SWO2aY\heIlwdiCxZjDoeY1idiCHUnHsdIhiKGW6cILld5Nm\CCrbjDoeY1idiCXMj3PV{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGVEPTB;MD6wNFAxODUQvF2= NIjZbFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C0N|I1Oid-MkOwOFMzPDJ:L3G+
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CV-1 NX\5fld6VHWlaX\ldoF{\SCDc4PhfS=> M1HWPGx2[2moZYLhd4UhSXO|YYm6JGV{fHKxZ3XuJJJm[2WydH;yMY5m\2G2aY\lJGNXNTFia3nkcoV6KGOnbHzzJIFz\SCvYXnueIFqdmWmIHnuJGR2dGKnY3PvK5MhdW:maX\p[YQhTWGpbHWnd{Bu\WSrdX2ge4l1cCB2LkWg[{9NKGeudXPvd4Uhe3WycHzlcYVvfGWmIIfpeIghOTBnIH\leIFtKGKxdnnu[UB{\XK3bTDhcoQhOTByIIXubZR{N22uIIDlcolkcWyuaX6td5Rz\XC2b335Z4lvKGG2IEO3xtAhSy5iaX6gZUBpfW2rZHnmbYVlKDVnIFPPNkBifG1uIFnDOVA:OC5yMEG5{txO NGDYVoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
COS7 NIe5TGZHfW6ldHnvckBie3OjeR?= MWHDc41x\XSrdHn2[UBjcW6maX7nJIFn\mmwaYT5JJRwKEdvcILveIVqdiClb4XwcIVlKGW|dILv[4VvKHKnY3XweI9zKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZpkhTTJiQXzlfIEhPjN|IIP0ZYlvcW6pIHLhd4VlKG[udX;y[ZNk\W6lZTDhcoFtgXOrczygT4k:OC5yMEK3{txO MnuwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
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UMR106 NVnhWnNjTnWwY4Tpc44h[XO|YYm= M3LUcVExKG6P Ml;sNlQhcHK| NHj0R3pVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gSXIu[WyyaHGgeJJidnOoZXP0[YQhcW5icnH0JHVOWjFyNjDj[YxteyCjdDCxNEcuQCCPIHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? M4DGblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{PS9pPlPoSW1DVDxxYU6=
UMR106 M3PubWZ2dmO2aX;uJIF{e2G7 NYLUWW9DOTBibl2= NXPUNm83OjRiaILz MofDWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIFXSMYJmfGFidILhcpNn\WO2ZXSgbY4hemG2IGXNVlExPiClZXzsd{BifCBzMDetPEBOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> Mn2wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
U2OS NHLvV4RHfW6ldHnvckBie3OjeR?= M4rIN|AvODFidV2= NEXRU5ozPCCqcoO= NHroU3FC[3SrdnH0bY9vKG:oIFXSZoV1[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCXMl;TJINmdGy|IHH0JFAvODFidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 MmLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
U2OS MnvrSpVv[3Srb36gZZN{[Xl? MYGwMlAyKHWP MYSyOEBpenN? M4jqR2FkfGm4YYTpc44hd2ZiRWLhcJBp[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCXMl;TJINmdGy|IHH0JFAvODFidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 Mn7aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
体内研究 Estradiol调节成年大鼠发情周期内海马突触密度的波动。[4] Estradiol可以独自扭转卵巢切除引发的脊椎密度的减少。Estradiol结合Progesterone先增加脊椎密度,为期2至6小时,但随后产生比单独Estradiol处理更大的降低。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 54 mg/mL (198.25 mM)
Water Insoluble
Ethanol '5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 272.38
化学式

C18H24O2

CAS号 50-28-2
储存条件 粉状
溶于溶剂
别名 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05175105 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05144256 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05096169 Not yet recruiting Drug: Clomiphene Citrate 25mg|Drug: Clomiphene Citrate 50mg Testosterone Deficiency Weill Medical College of Cornell University December 2021 Phase 2

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在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID