RSL3
目录号：S8155
Molecular Weight(MW): 440.88
RSL3是一种ferroptosis的激活剂，不依赖于VDAC，对携带致瘤性RAS的肿瘤细胞具有选择性。它结合并使GPX4失活，介导GPX4所调节的铁死亡。
客户使用该产品的6个实验数据：
-
Fluvastatin treatment of HT-1080 cells decreases GPX4 protein levels in a time- and concentration-dependent manner and synergized with co-treatment with the direct GPX4 inhibitor RSL3.
Nature, 2017, 547(7664):453-457. RSL3 purchased from Selleck.
HK2 cells with elevated PEBP1 are more sensitive to ferroptosis. Inset: ferrostatin-1-inhibitable cell death (means ± SD, *p < 0.05 versus pCMV6, **p < 0.05 versus pCMV6/RSL3, ***p < 0.05 versus pCMV6-PEBP1/RSL3, #p < 0.05 versus pCMV6/RSL3, n = 3/group). Inset: quantitation of ferroptosis as differences in LDH in ± FER-treated cells.
Cell, 2017, 171(3):628-641. RSL3 purchased from Selleck.
-
ATG3 is required for ferroptosis. ATG3KO MEFs, or ATG3KO cells reconstituted with ectopic ATG3 expression, were treated as indicated for 12 h. Cell death was determined by PI staining coupled with flow cytometry. ATG3 expression was confirmed by immunoblotting. erastin: 1 μM; RSL3: 1 μM.
Cell Res, 2016, 26(9):1021-32. RSL3 purchased from Selleck.
Detection of lipid hydroperoxides by live cell fluorescence imaging of Liperfluo in HBE cells treated with RSL3 (200 nM) or ΔwspF supernatant for 4 hours. Top panel shows time course of changes in the fluorescence intensity from baseline. Bottom panel shows typical changes in fluorescence (of 4 performed) in one stage position (of 10) at 3 time points (0, 2, and 4 hours). Time control used as a negative control and RSL3 as a positive control for ferroptosis. For statistical analysis, each stage position was counted as one data entry.
J Clin Invest, 2018, 128(10):4639-4653. RSL3 purchased from Selleck.
-
miR-137 negatively regulates ferroptosis in melanoma. a The A375 and G-361 human melanoma cells were treated with erastin (2.5–40 µM) or RSL3 (0.1–10 µM) with or without a cell death inhibitor (ferrostatin-1, 1 µM; ZVAD-FMK, 10 µM; necrosulfonamide, 0.5 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. ****p < 0.0001. b Overexpression of miR-137 suppressed erastin- and RSL3-induced cell death in human melanoma cells. Indicated cells were treated with erastin (2.5–40 µM) or RSL3 (0.1–10 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001. c Inhibition of endogenous miR-137 enhanced erastin- and RSL3-induced cell death in human melanoma cells. Indicated cells were treated with erastin (2.5–40 µM) or RSL3 (0.1–10 µM) for 24 h. Cell death was assayed using a CCK-8 kit. Data shown represent mean ± SD from three independent experiments. *p < 0.05; **p < 0.01. d Quantitative RT-PCR showing the relative expression levels of miR-137 in b. Data are mean ± SD from three independent experiments. ***p < 0.001. e Quantitative RT-PCR showing the relative expression levels of miR-137 in d. Data are mean ± SD from three independent experiments. ***p < 0.001
Cell Death Differ, 2018, 1457-1472. RSL3 purchased from Selleck.
(D) Representative western blot and (E) densitometry showing protein levels of HO-1 in HO-1+/+ PTCs treated with RSL3 over time. Data shown represent the Mean ± SEM of three independent experiments performed each time in triplicate; * = p<0.05, ** = p<0.01 compared to lower concentration at each time point.
Am J Physiol Renal Physiol, 2017, doi: 10.1152/ajprenal.00044.2017. RSL3 purchased from Selleck.
产品安全说明书
Ferroptosis抑制剂选择性比较
生物活性
| 产品描述 | RSL3是一种ferroptosis的激活剂，不依赖于VDAC，对携带致瘤性RAS的肿瘤细胞具有选择性。它结合并使GPX4失活，介导GPX4所调节的铁死亡。 | |
|---|---|---|
| 靶点 |
|
|
| 体外研究 |
可诱导铁死亡的化合物通过直接结合或减少谷胱甘肽来使GPX4失活。结合GPX4后，RSL3使GPX4失活、诱导脂质过氧化而诱发的活性氧生成。RSL3可十分快速有效地诱导与致瘤性RAS的协同致死。低浓度（10 ng/ml）RSL3抑制BJ-TERT/LT/ST/RASV12以及DRD细胞的生长，在处理后8小时可杀死敏感细胞[1][2]。 |
|
| 体内研究 | 前列腺素内过氧化物合成酶(PTGS)是前列腺素合成中的一种关键的酶。组成型PTGS1和诱导型PTGS2是PTGS的两种同功酶。在小鼠中，处理以RSL3和erastin后可显著上调由PTGS2编码的COX-2水平[1]。 |
推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)
| 细胞实验： |
+ 展开
|
|---|---|
| 动物实验： |
+ 展开
|
溶解度 (25°C)
| 体外 | DMSO | 88 mg/mL (199.6 mM) |
|---|---|---|
| Ethanol | 22 mg/mL (49.9 mM) | |
| Water | Insoluble | |
| 体内 |
从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+2% Tween 80+ddH2O |
2mg/mL |
* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。
化学数据
| 分子量 | 440.88 |
|---|---|
| 化学式 | C23H21ClN2O5 |
| CAS号 | 1219810-16-8 |
| 稳定性 | powder |
| in solvent | |
| 别名 | N/A |
计算器
摩尔浓度计算器
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。
稀释计算器
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )
在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.
分子量计算器
通过输入化合物的化学式来计算其分子量：
注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2
摩尔浓度计算器
技术支持
在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。
如果有其他问题，请给我们留言。
