Casein Kinase
抑制剂选择性比较
特异性亚型抑制剂
Casein Kinase产品
| 目录号 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| S1105 |
LY294002LY294002 (SF 1101, NSC 697286) 是首个合成的已知抑制PI3Kα/δ/β的小分子，在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM；在溶液中比在Wortmannin中稳定，也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶，还能够结合一些与PI3K家族无关的新型靶点。LY294002 可以抑制CK2，对应的IC50值为98 nM。LY294002 是一种非特异的 DNA-PKcs 抑制剂，并可激活自噬和凋亡。 |
Inhibition of PI3K, ERK and mTOR prevents the activation of S6K1 and S6 induced by suppression of PKD1 activity. A549 cells were incubated in the absence (-) or presence of either 5 uM Kb or 5 uM Kb and 20 uM LY294002 or 5 uM Kb and 10 uM BKM120 (as indicated) for 1 h prior to stimulation of cells with 50 nM PMA for 30 min and 1 h.
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| S2248 |
Silmitasertib (CX-4945)Silmitasertib (CX-4945)是有效的，选择性CK2(casein kinase 2)抑制剂，无细胞试验中IC50为1 nM,对 Flt3, Pim1 和 CDK1作用效果稍弱(细胞试验中没有活性)。Silmitasertib可诱导自噬并促进细胞凋亡。Phase 1/2。 |
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| S2805 |
LY364947LY364947 (HTS 466284)是一种有效的ATP竞争性TGFβR-I抑制剂，无细胞试验中IC50为59 nM，比作用于TGFβR-II选择性高7倍。 |
The cytoplasmic and nuclear proteins were separated and the protein expression levels were determined by performing western blotting. LY364947 (1 uM), which is a potent ATP-competitive inhibitor of TGF-βRI, was used as the positive control. GAPDH and PARP were used as cytosolic and nuclear markers, respectively. |
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| S7085 |
IWP-2IWP-2是一种Wnt信号和分泌抑制剂，无细胞试验中IC50为27 nM，选择性阻断Porcn介导的Wnt棕榈酰化，一般不影响Wnt/β-catenin，且对Wnt刺激的细胞反应没有影响。IWP-2可特定低抑制 CK1δ。 |
KIF3A knockdown promotes both ligand secretion and intracellular signalling of the Wnt/β-catenin pathway in NSCLC cell lines. (c,d) SuperTOPFlash reporter assay to examine the effect of KIF3A knockdown on signalling pathway activation by autocrine or exogenous WNTs in A549 and SW900 cell lines. A549 and SW900 cells were incubated with DMSO or IWP-2 (30 μ M) for 48 h, and then treated with BSA or WNT3A (100 ng/ml) for additional 24 h. Luciferase reporter activity was calculated by dividing TOP/RL ratio by FOP/RL ratio (n = 3; error bars indicate SEM; p-value was determined by one-way ANOVA). *P < 0.05; **P < 0.01.
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| S0707 |
Silmitasertib (CX-4945) sodium saltSilmitasertib (CX-4945) sodium salt是一种有效的、口服可生物利用的、选择性的 casein kinase 2 (CK2) 的抑制剂，对CK2α和CK2α'的IC50值为1 nM。 |
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| S9850New |
(E/Z)-GO289(E/Z)-GO289 在体外激酶测定实验中以 7 nM 的 IC50 有效且选择性地抑制 casein kinase 2 (CK2)。 |
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| S6544 |
SR-3029SR-3029是有效的、选择性casein kinase 1δ/1ε (CK1δ/ε)抑制剂，对CK1δ和CK1ε的IC50值分别为44 nM和260 nM。 |
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| S5265 |
TBBTBB (4,5,6,7-tetrabromobenzotriazole) is a selective cell-permeable CK2 inhibitor with IC50 values of 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively. |
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| S6536 |
TTP 22TTP22是casein kinase 2 (CK2)抑制剂，IC50值为100 nM。它对CK2具有选择性，在浓度为10 μM时，对JNK3, ROCK1和MET没有抑制活性。 |
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| S6578 |
LongdaysinLongdaysin是Casein Kinase抑制剂，对CKIδ, CKIα, ERK2和CDK7的IC50值分别为8.8 μM, 5.6 μM, 52 μM和29 μM。 |
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| S2249 |
PF 4800567PF 4800567是casein kinase 1 epsilon (IC50=32 nM) 的新型有效抑制剂，其选择性是casein kinase 1 delta(IC50=711 nM)的20倍以上。 |
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| S0350 |
CK1-IN-1CK1-IN-1 (PUN51207, Compound 1C) 是 casein kinase 1 (CK1) 的抑制剂，对CK1δ、CK1ε和p38αMAPK的IC50值分别为15 nM、16 nM和73 nM。 |
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| S0995 |
BioE-1115BioE-1115 是一种有效的、高度选择性的 PAS kinase (PASK) 和 casein kinase 2α (CK2α) 的双重抑制剂，IC50分别为约4 nM和约10μM。 |
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| S6734 |
PF-670462PF-670462是有效的、选择性CK1ε和CK1δ抑制剂，IC50分别为90 nM和13 nM。 |
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| S7642 |
D 4476D 4476 是一种有效的，选择性的，细胞渗透性 CK1 (casein kinase 1) 抑制剂，无细胞测定中对来自粟酒裂殖酵母的 CK1 和 CK1δ，IC50 分别为 200 nM 和 300 nM 。也是一种 ALK5 抑制剂，IC50 为 500 nM。 |
Western blot of signaling effects. 48 hrs after transfection cells were serum‐starved for 12 h before stimulation with Wnt5a for 30 min or 120 min (200 ng/ml) in the presence or absence of casein kinase inhibitor D4476 (100 μM).
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| S8237 |
IC261IC261 (SU-5607)是一种新型的CK1抑制剂，IC261对CK1的IC50为16 μM，对Cdk5的IC50为4.5 mM。 |
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| S0186 |
TA-01TA-01 是 CK1δ/ε 和 p38 MAPK 的有效抑制剂，对 CK1ε、CK1δ 和 p38 MAPK 的 IC50 分别为 6.4 nM、6.8 nM 和 6.7 nM。 |
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| S5516 |
Ellagic Acid hydrateEllagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties. |
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| S3143 |
Pyruvic acidPyruvic acid (Acetylformic acid) 是一种重要的有机化学中间体，在心肌细胞的病理生理和治疗中发挥作用。Pyruvic acid 可显着提高 lactate dehydrogenase (LDH) 和 creatine kinase (CK) 的水平，并降低 Ca2+Mg2+-ATPase 和 Na+K+-ATPase 的水平。 |
| 目录号 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| S1105 |
LY294002LY294002 (SF 1101, NSC 697286) 是首个合成的已知抑制PI3Kα/δ/β的小分子，在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM；在溶液中比在Wortmannin中稳定，也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶，还能够结合一些与PI3K家族无关的新型靶点。LY294002 可以抑制CK2，对应的IC50值为98 nM。LY294002 是一种非特异的 DNA-PKcs 抑制剂，并可激活自噬和凋亡。 |
Inhibition of PI3K, ERK and mTOR prevents the activation of S6K1 and S6 induced by suppression of PKD1 activity. A549 cells were incubated in the absence (-) or presence of either 5 uM Kb or 5 uM Kb and 20 uM LY294002 or 5 uM Kb and 10 uM BKM120 (as indicated) for 1 h prior to stimulation of cells with 50 nM PMA for 30 min and 1 h.
|
|
| S2248 |
Silmitasertib (CX-4945)Silmitasertib (CX-4945)是有效的，选择性CK2(casein kinase 2)抑制剂，无细胞试验中IC50为1 nM,对 Flt3, Pim1 和 CDK1作用效果稍弱(细胞试验中没有活性)。Silmitasertib可诱导自噬并促进细胞凋亡。Phase 1/2。 |
|
|
| S2805 |
LY364947LY364947 (HTS 466284)是一种有效的ATP竞争性TGFβR-I抑制剂，无细胞试验中IC50为59 nM，比作用于TGFβR-II选择性高7倍。 |
The cytoplasmic and nuclear proteins were separated and the protein expression levels were determined by performing western blotting. LY364947 (1 uM), which is a potent ATP-competitive inhibitor of TGF-βRI, was used as the positive control. GAPDH and PARP were used as cytosolic and nuclear markers, respectively. |
|
| S7085 |
IWP-2IWP-2是一种Wnt信号和分泌抑制剂，无细胞试验中IC50为27 nM，选择性阻断Porcn介导的Wnt棕榈酰化，一般不影响Wnt/β-catenin，且对Wnt刺激的细胞反应没有影响。IWP-2可特定低抑制 CK1δ。 |
KIF3A knockdown promotes both ligand secretion and intracellular signalling of the Wnt/β-catenin pathway in NSCLC cell lines. (c,d) SuperTOPFlash reporter assay to examine the effect of KIF3A knockdown on signalling pathway activation by autocrine or exogenous WNTs in A549 and SW900 cell lines. A549 and SW900 cells were incubated with DMSO or IWP-2 (30 μ M) for 48 h, and then treated with BSA or WNT3A (100 ng/ml) for additional 24 h. Luciferase reporter activity was calculated by dividing TOP/RL ratio by FOP/RL ratio (n = 3; error bars indicate SEM; p-value was determined by one-way ANOVA). *P < 0.05; **P < 0.01.
|
|
| S0707 |
Silmitasertib (CX-4945) sodium saltSilmitasertib (CX-4945) sodium salt是一种有效的、口服可生物利用的、选择性的 casein kinase 2 (CK2) 的抑制剂，对CK2α和CK2α'的IC50值为1 nM。 |
||
| S9850New |
(E/Z)-GO289(E/Z)-GO289 在体外激酶测定实验中以 7 nM 的 IC50 有效且选择性地抑制 casein kinase 2 (CK2)。 |
||
| S6544 |
SR-3029SR-3029是有效的、选择性casein kinase 1δ/1ε (CK1δ/ε)抑制剂，对CK1δ和CK1ε的IC50值分别为44 nM和260 nM。 |
||
| S5265 |
TBBTBB (4,5,6,7-tetrabromobenzotriazole) is a selective cell-permeable CK2 inhibitor with IC50 values of 0.9 and 1.6 μM for rat liver and human recombinant CK2 respectively. |
||
| S6536 |
TTP 22TTP22是casein kinase 2 (CK2)抑制剂，IC50值为100 nM。它对CK2具有选择性，在浓度为10 μM时，对JNK3, ROCK1和MET没有抑制活性。 |
||
| S6578 |
LongdaysinLongdaysin是Casein Kinase抑制剂，对CKIδ, CKIα, ERK2和CDK7的IC50值分别为8.8 μM, 5.6 μM, 52 μM和29 μM。 |
||
| S2249 |
PF 4800567PF 4800567是casein kinase 1 epsilon (IC50=32 nM) 的新型有效抑制剂，其选择性是casein kinase 1 delta(IC50=711 nM)的20倍以上。 |
||
| S0350 |
CK1-IN-1CK1-IN-1 (PUN51207, Compound 1C) 是 casein kinase 1 (CK1) 的抑制剂，对CK1δ、CK1ε和p38αMAPK的IC50值分别为15 nM、16 nM和73 nM。 |
||
| S0995 |
BioE-1115BioE-1115 是一种有效的、高度选择性的 PAS kinase (PASK) 和 casein kinase 2α (CK2α) 的双重抑制剂，IC50分别为约4 nM和约10μM。 |
||
| S6734 |
PF-670462PF-670462是有效的、选择性CK1ε和CK1δ抑制剂，IC50分别为90 nM和13 nM。 |
||
| S7642 |
D 4476D 4476 是一种有效的，选择性的，细胞渗透性 CK1 (casein kinase 1) 抑制剂，无细胞测定中对来自粟酒裂殖酵母的 CK1 和 CK1δ，IC50 分别为 200 nM 和 300 nM 。也是一种 ALK5 抑制剂，IC50 为 500 nM。 |
Western blot of signaling effects. 48 hrs after transfection cells were serum‐starved for 12 h before stimulation with Wnt5a for 30 min or 120 min (200 ng/ml) in the presence or absence of casein kinase inhibitor D4476 (100 μM).
|
|
| S8237 |
IC261IC261 (SU-5607)是一种新型的CK1抑制剂，IC261对CK1的IC50为16 μM，对Cdk5的IC50为4.5 mM。 |
||
| S0186 |
TA-01TA-01 是 CK1δ/ε 和 p38 MAPK 的有效抑制剂，对 CK1ε、CK1δ 和 p38 MAPK 的 IC50 分别为 6.4 nM、6.8 nM 和 6.7 nM。 |
||
| S5516 |
Ellagic Acid hydrateEllagic acid is a potent inhibitor of protein kinase CK2 with IC50s of 0.04, 2.9 and 3.5 μM for CK2, Lyn and PKA respectively. It shows potent antioxidant, anti-mutagenic and antidepressant properties. |
| 目录号 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| S3143 |
Pyruvic acidPyruvic acid (Acetylformic acid) 是一种重要的有机化学中间体，在心肌细胞的病理生理和治疗中发挥作用。Pyruvic acid 可显着提高 lactate dehydrogenase (LDH) 和 creatine kinase (CK) 的水平，并降低 Ca2+Mg2+-ATPase 和 Na+K+-ATPase 的水平。 |
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