FCCP

别名: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone 中文名称:碳酰氰-4-三氟甲氧基苯腙

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在线粒体中是一种有效的氧化磷酸化的解偶联剂,通过转运质子破坏ATP的合成。

FCCP Chemical Structure

FCCP Chemical Structure

CAS: 370-86-5

规格 价格 库存 购买数量
10mg RMB 794.76 现货
50mg RMB 2841.98 现货
200mg RMB 6527.44 现货
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客户使用Selleck的FCCP发表文献52

产品质控

批次: 纯度: 99.99%
99.84

FCCP相关产品

OXPHOS抑制剂选择性比较

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
T47D Function assay 0.3 uM 15 mins Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay 20929261
T47D Function assay 3 uM 15 to 20 mins Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay 22938093
SH-SY5Y Function assay 10 uM 5 mins Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay 25265024
SH-SY5Y Function assay 10 uM 10 mins Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay 25265024
T47D Function assay 0.3 uM 3 to 12 mins Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay 26637046
T47D Function assay 0.3 uM 30 mins Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy 26637046
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method 28233680
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method 28233680
KOPN8 Function assay 10 uM 0.3 hrs Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis 31084028
T47D Function assay 1 to 10 uM Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA 20929261
MDA-MB-231 Cytotoxicity assay 1 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM 23245650
MDA-MB-231 Cytotoxicity assay 0.1 to 3 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor 23245650
MDA-MB-231 Function assay 0.3 uM Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM 23245650
T47D Function assay 0.1 to 3 uM Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM 22938093
T47D Function assay 10 to 30 uM Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin 22938093
T47D Function assay 0.3 to 1 uM Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin 22938093
Hep3B Function assay 10 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin 22938093
Hep3B Function assay 1 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin 22938093
TA3/Ha Function assay 6 uM Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis 24568614
T47D Function assay 0.3 uM Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate 26637046
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
HEK293 Function assay 1 to 3 uM Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay 31774672
T47D Function assay Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM 20929261
T47D Function assay Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM 20929261
HepG2 Function assay Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM ChEMBL
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生物活性

产品描述 FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在线粒体中是一种有效的氧化磷酸化的解偶联剂,通过转运质子破坏ATP的合成。
靶点
OXPHOS [2] ATP synthase [2]
体外研究(In Vitro)
体外研究活性

在体外实验中,FCCP的处理能诱导细胞内Ca2+迅速升至2倍,同时抑制蛋白合成速率。对蛋白翻译速率的抑制作用与eIF2α的磷酸化增加以及依赖双链RNA的蛋白激酶活性增加有关[1]

FCCP还可轻微地减少ATP以及活性氧水平,提高线粒体基因如Tfam和COXIV的表达,诱导老鼠造血干细胞的静态形态特征以及抑制TGF-β的信号转导[2]

细胞实验 细胞系 PC12细胞
浓度 30 μM
孵育时间 30 min, 1h, 2h
方法

用直径为24-mm的多孔板,加入含0.175 Ci/mmol的[3H]methionine的新鲜培养液,37℃孵育30分钟。在不同的时间段对PC12细胞处理以FCCP,测定蛋白质合成速率。

体内研究(In Vivo)
体内研究活性

FCCP的处理在8细胞期的小鼠胚胎中可显著降低线粒体膜电位、ATP的生成以及囊胚中细胞内细胞团的数量,对胚泡发育没有影响。在胚胎线粒体功能受到干扰的同时,在胚胎植入后,雌性后代的出生体重下降,抑制持续到断奶阶段。尽管FCCP处理过的雄性小鼠也和雌性小鼠一样,葡萄糖耐受量降低。但是它们的胰岛素敏感度和肥胖度在4-14周内无变化。在预压胚中,线粒体功能降低,然后减少ATP的输出,将影响其后代表型[3]

化学信息&溶解度

分子量 254.17 分子式

C10H5F3N4O

CAS号 370-86-5 SDF Download FCCP SDF
Smiles C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 6 mg/mL ( 23.6 mM; DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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