Colforsin

目录号:S2449 别名: Forskolin, HL 362, Coleonol 中文名称:佛司可林

仅限科研使用

Colforsin (Forskolin, HL 362, Coleonol)在各种各样细胞类型中,是一种普遍存在的真核细胞腺苷酸环化酶(AC)激活剂,在细胞生理学研究中,通常用来提高cAMP水平。Forskolin 还可激发 PXRFXR的活性而诱导自噬。

Colforsin  Chemical Structure

CAS: 66575-29-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1144.16 现货
RMB 903.37 现货
RMB 3029.97 现货
RMB 4679.71 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Colforsin 发表文献111篇:

产品安全说明书

cAMP抑制剂选择性比较

生物活性

产品描述 Colforsin (Forskolin, HL 362, Coleonol)在各种各样细胞类型中,是一种普遍存在的真核细胞腺苷酸环化酶(AC)激活剂,在细胞生理学研究中,通常用来提高cAMP水平。Forskolin 还可激发 PXRFXR的活性而诱导自噬。
靶点
Adenylyl cyclase (AC) [1]
(A wide variety of cell types)
体外研究

Forskolin可以使膜,细胞或组织制备液中cAMP含量上升。Forskolin 不仅可以活化AC 而且可以与某些其它蛋白相互作用,包括葡萄糖转运蛋白和离子通道。Forskolin能够促进9种不同独立AC形式的活化,虽然对AC9效率有点低,这可以用于提供一种鉴定和量化G蛋白 (Gs)–AC复合物高亲和性结合位点的方法。G蛋白偶联受体活化G蛋白有助于细胞中Forskolin刺激的cAMP 产生,因为 G蛋白-Forskolin对AC活性有增强作用[1]。Forskolin在不与细胞表面受体相互作用前提下刺激腺苷酸环化酶活性。在脂肪细胞中Forskolin's促进 cAMP产生进而可以抑制嗜碱性粒细胞和肥大细胞脱颗粒作用以及组胺释放,降低血压、眼内压,抑制血小板聚集,促进血管舒张,支气管扩张和甲状腺激素分泌,刺激脂肪分解。Forskolin 抑制血小板活化因子 (PAF)的结合, 这种抑制不依赖于cAMP形成可能是 Forskolin's 直接作用于 PAF或者通过干扰PAF与它的受体结合实现的。Forskolin似乎对多种膜转运蛋白也有效果并且可以抑制脂肪细胞,红细胞,血小板,和其他细胞中的葡萄糖运输。Forskolin也被用于治疗青光眼[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-29  M4rGXmFxd3C2b4Ppd{BCe3OjeR?= M{HvUFQxyqEQvF2= NX\S[npwPDkEoHi= NVj4e|R{TE2VTx?= MYXpcoR2[2W|IHPoZY5o\XNiaX6geIhmKHCqb4PwbI9zgWyjdHnvckB{fGG2dYOgc4YhWFB{QTD0ZZJo\XS| MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7N{S1NUc,OjR7OUe0OVE9N2F-
SW480 NUn3OIpVSXCxcITvd4l{KEG|c3H5 NHnUPJg1OMLizszN NXLUdZNVPDkEoHi= M3nSbmROW09? M1H5eYlv\HWlZYOgZ4hidmenczDpckB1cGVicHjvd5Bpd3K7bHH0bY9vKHO2YYT1d{Bw\iCSUELBJJRiemendIO= MoO2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUe0OVEoRjJ2OUm3OFUyRC:jPh?=
HT-29  NXj6RmxlSXCxcITvd4l{KEG|c3H5 NVr6bmZ[PDEEoN88US=> MkHEOFjDqGh? MUfEUXNQ NYXyZoI6cW6mdXPld{BidiCjY4TpeoF1cW:wIH;mJINie3Cjc3WgN{84 NGXz[Hg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5O|Q2OSd-MkS5PVc1PTF:L3G+
SW480 MVTBdI9xfG:|aYOgRZN{[Xl? Mnn4OFDDqM7:TR?= Mnu1OFjDqGh? M2j0Z2ROW09? NEHoSoxqdmS3Y3XzJIFvKGGldHn2ZZRqd25ib3[gZ4F{eGG|ZTCzM|c> M371flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm3OFUyLz5{NEm5O|Q2OTxxYU6=
HT-29  MnHWSpVv[3Srb36gRZN{[Xl? M{jH[FQxyqEQvF2= MmLJO{Bl NYHPcWU6TE2VTx?= NWrSVW45emWmdXPld{Bkd2yxbn;zdIhmemViZn;ycYF1cW:wIHPhdIFjcWyrdIpCpC=> M3jBOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm3OFUyLz5{NEm5O|Q2OTxxYU6=
SW480 MUXGeY5kfGmxbjDBd5NigQ>? MlHyOFDDqM7:TR?= MmXxO{Bl NFHZfI5FVVOR M1uwcpJm\HWlZYOgZ49td26xc4Do[ZJmKG[xcn3heIlwdiClYYDhZoltcXS7wrC= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7N{S1NUc,OjR7OUe0OVE9N2F-
HT-29  MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDROFDDqM7:TR?= NVnDXollOC15MjDo M3r6fGROW09? Mn3YbY5pcWKrdIOgZ4VtdCCpcn;3eIghfGmvZTDk[ZBmdmSnboTsfS=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7N{S1NUc,OjR7OUe0OVE9N2F-
SW480 NH3YTmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDNd|hwPDEEoN88US=> M4ixSVAuPzJiaB?= NFnxWotFVVOR NGP2RYVqdmirYnn0d{Bk\WyuIHfyc5d1cCC2aX3lJIRmeGWwZHXueIx6 MlPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUe0OVEoRjJ2OUm3OFUyRC:jPh?=
HT-29  M4TUcGZ2dmO2aX;uJGF{e2G7 MUS0NOKh|ryP MmfBOFjDqGh? MoqzSG1UVw>? MnKyZYN1cX[jdHXzJHBROkF? M2jSZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm3OFUyLz5{NEm5O|Q2OTxxYU6=
SW480 NFHqOJhHfW6ldHnvckBCe3OjeR?= NFu0bXc1OMLizszN Mo\MOFjDqGh? NXTXPFlbTE2VTx?= MkDSZYN1cX[jdHXzJHBROkF? MlrBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUe0OVEoRjJ2OUm3OFUyRC:jPh?=
C6 M2n2emZ2dmO2aX;uJGF{e2G7 NGXNfJIyOCEQvF5CpC=> M4HUPFIxKG2rbh?= M1:3NIlv[3KnYYPld{BkSU2SIHHjZ5VufWyjdHnvci=> NEmwfnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2PVQyPyd-MkWwOlk1OTd:L3G+
Huh-7 Mo\CSpVv[3Srb36gRZN{[Xl? NGXaUJMxNTJyIN88US=> NVPseYgyOiCqwrC= MVTy[ZN2dHS|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgbY5kemWjc3WgbY4h[y2PeXOg[ZhxemW|c3nvckBifCC2aHWgdJJwfGWrbjDhcoQhdVKQQTDs[ZZmdHN? M2TQW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUC5PFM1Lz5{NUGwPVg{PDxxYU6=
THP-1  MUPGeY5kfGmxbjDBd5NigQ>? Mnv5NU8yOCEQvF2= NEfjcpEzyqCq MlHVSG1UV8Li NVjIUJF[e3WycILld5NmeyCPQ2CtNUBxem:mdXP0bY9vyqB? MlywQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNUS4PFIoRjJ3MUW0PFgzRC:jPh?=
BeWo  MWPGeY5kfGmxbjDBd5NigQ>? NGjhcnEzOCEEtV2= NXz0[GJ6PDhiaB?= NHX2TJpFVVORwrC= NXzBflExcW6mdXPld{Bk\WyuIH\1d4lwdg>? NUj2cpYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPFQ1PzdpPkK1NVg1PDd5PD;hQi=>
ventricular cardiomyocytes  M13sN2Z2dmO2aX;uJGF{e2G7 MVWwMlAyNTFyIN88US=> NVPFXZVU\X[xa3XzJIFvKGmwb4Tyc5Bq[yC{ZYPwc45{\SBzMkFCtVE2LSCjYn;2[UBj[XOjbDD3bZRpKGGwIFXDOVDDqG:oIEKuNkDDvU1? MofrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MEOxNVMoRjJ3MkCzNVE{RC:jPh?=
ventricular cardiomyocytes  M33pfmZ2dmO2aX;uJGF{e2G7 MWmwMlAyNTFyIN88US=> MmHHbY5kemWjc3XzJEBkSU2SIHHjZ5VufWyjdHnvci=> M{PSOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkCzNVE{Lz5{NUKwN|EyOzxxYU6=
MIN6  MWPGeY5kfGmxbjDBd5NigQ>? M4G1dFExKM7:TdMg NHK2VmY{KGh? MXfpcoNz\WG|ZYOgSFMhdVKQQTDlfJBz\XO|aX;u NEm1SnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK0NVEzPCd-MkWyOFEyOjR:L3G+
L6 NHzRfVBHfW6ldHnvckBCe3OjeR?= MXS0NEDDvU1? NVjO[lVKOjRiaB?= NGX6W|lqdmirYnn0d{BFVUhzLXnu[JVk\WRiQXv0JIFkfGm4YYTpc44> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ2N{W1NEc,OjV{NEe1OVA9N2F-
HEK‐CFTR Ml6wSpVv[3Srb36gRZN{[Xl? M3rrbVLjiJN3MNMg{txO M3vMZVAuOTJibXnu NV7YV5ZKTE2VT9Mg MknTbY5lfWOnczDhJIRwe2YkgKDk[ZBmdmSnboSgbY9lcWSnIHXm[ox2gMLi NWrJW2FDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlMzODdpPkK1NlY{OjB5PD;hQi=>
SK-N-SH NIDFOWtE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4e1UlExKM7:TdMg NXHFPG5FPDhiaB?= MWLlcohidmOnczDTT{1PNVOKIH7leZJw[myjc4TvcYEh[2WubDD2bYFjcWyrdIm= NWHRVI54RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlYxPjNpPkK1NlY3ODZ|PD;hQi=>
SK-N-AS  NIPZdXNHfW6ldHnvckBCe3OjeR?= MoOzNVAh|ryPwrC= MV[xNE8{OC94MDDtbY4> NVm0cph2cW6lcnXhd4V{KGyndnXsd{Bw\iCyLd8yMYNifGWwaX6gLJNmejZ5NTmgZY5lKGmwZIXj[ZMh[WOldX31cIF1cW:wIH;mJJAu|rJvY3H0[Y5qdiBqc3XyOlc2MSCrbjCodIVzcSmwdXPs[YFzKHKnZ3nvcpM> Ml7SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{Nk[wOlMoRjJ3Mk[2NFY{RC:jPh?=
SK-N-AS  MULGeY5kfGmxbjDBd5NigQ>? NH7l[3YyOCEQvF5CpC=> MljBN|AhdWmw MYDpcoR2[2W|IIDoc5NxcG:{eXzheIlwdiCxZjFOtk1k[XSnbnnuJEh{\XJ4N{WpMEBxNUeVS{ROtkApe2W{OTmgZY5lKGOxbnPvcYl1[W62IHjp[4hmeiCuZY\lcJMhd2ZiYXP0bZZmNCC3boDoc5NxcG:{eXzheIVlNCEQsj3jZZRmdmmw NFntPY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2OlA3Oyd-MkWyOlYxPjN:L3G+
SK-N-AS  MXzGeY5kfGmxbjDBd5NigQ>? MVOxNEDPxE4EoB?= M{XOO|I1KGh? MkP6bY5kemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJIN6[2yrbjDENS=> NXfze5piRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlYxPjNpPkK1NlY3ODZ|PD;hQi=>
SK-N-AS  NFn1Z5FHfW6ldHnvckBCe3OjeR?= NEj0XIwyOCEQvF5CpC=> NX3yV4RVOjRiaB?= MVzpcoNz\WG|ZYOgeIhmKGODTWCgcIV3\Wy|wrC= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ4NkC2N{c,OjV{Nk[wOlM9N2F-
SK-N-AS  NE\Ue2xE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MmjhNVAh|ryPwrC= NELVdpgzPC92ODDo NXHLcnR4\W6qYX7j[ZMhfGmvZT3k[ZBmdmSnboTsfUBk\WyudXzhdkB3cWGkaXzpeJnDqA>? NY\Rb2hnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOlYxPjNpPkK1NlY3ODZ|PD;hQi=>
granulosa cells MYnGeY5kfGmxbjDBd5NigQ>? NF7GfGMyOCEQvF2= NYf4e2pkOjRiaB?= MoPlbY5kemWjc3XzJJRp\SCuZY\lcJMhd2ZiUlfTNkBxem:vb4TldkBi[3Srdnn0fS=> NIm4WpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzPVExPSd-MkWzN|kyODV:L3G+
granulosa cells NYLTbHlkTnWwY4Tpc44hSXO|YYm= NUDxPFVyOTBizszN MkfaNlQhcA>? M2PjNolv[3KnYYPld{B1cGViaX70[Y5{cXS7IH;mJGRPSS:ycn;0[YlvKGOxbYDs[Zg> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|OUGwOUc,OjV|M{mxNFU9N2F-
granulosa cells NUjtcVFiTnWwY4Tpc44hSXO|YYm= NWGySHExOTBizszN NXfjd3NJOTJxMkSgbC=> M{T1colv[3KnYYPld{B1cGVibHX2[Yx{KG:oIILldI9zfGW{IHHjeIl3cXS7IH\vdkB1cGVibH;u[4V{fCCocnHncYVvfCBq4pkSPFU1NytzOGLHV|IvVFWFKR?= NEjz[no9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzPVExPSd-MkWzN|kyODV:L3G+
granulosa cells NWn3UYdVTnWwY4Tpc44hSXO|YYm= MVWxNEDPxE1? MWixNk8zPCCq M2rTVolv[3KnYYPld{B1cGVibHX2[Yx{KG:owrDSS3MzyqCvUl7B NUW5[|I1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN|kyODVpPkK1N|M6OTB3PD;hQi=>
BeWo  MlrHSpVv[3Srb36gRZN{[Xl? NWf2T|g2OjEEoNM1US=> NE\zZnY1QMLiaNMg MULEUXNQyqB? NF2xVGlld3ewcnXneYxifGW|IITo[UBt\X[nbDDv[kBIS01vMR?= NImz[ZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO2NlI3OCd-MkWzOlIzPjB:L3G+
BeWo  NXvzW3h5TnWwY4Tpc44hSXO|YYm= MXmyNOKhyrWP M2PIRVQ5yqCqwrC= MWLEUXNQyqB? NV7O[Ywy\G:5boLl[5Vt[XSnczD0bIUhdGW4ZXygc4YhXE2HTV[xOi=> M3rCTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{[yNlYxLz5{NUO2NlI3ODxxYU6=
BeWo  NH3zTnlHfW6ldHnvckBCe3OjeR?= Mk\QNlDDqML3TR?= NGXJb5g1QMLiaNMg Mn[zSG1UV8Li M17Zb4lv[3KnYYPld{B1cGViYnX0ZU1pS0dicnXs[YF{\Q>? NWTOeopmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOlIzPjBpPkK1N|YzOjZyPD;hQi=>
EM1  M{fuWWZ2dmO2aX;uJGF{e2G7 NF\abVEyPeLCit88US=> M4nWbVQ5KGh? NUDq[mk2emWmdXPld{B1cGViZYjwdoV{e2mxbjDv[uKhVEmIwrDvduKhWFSJU{NCpIlvyqCFQVzSMUBwesLiRWDBR|Iue2muZX7j[YQhTU1zIHPlcIx{yqB? M3;HWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{e4OlYyLz5{NUO3PFY3OTxxYU6=
Primary bovine chondrocytes M4XlR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXy1{txO NHqyRY01QCCq M1y0OZJmfmW{c3XzJJRp\SCrbnjpZol1d3K7IHXm[oVkfCCxZjDj[Yxm[2:6aXKgc44heHKxbHnm[ZJifGmxbjDpckBoem:5dHigdIxifGViY3jvcoRzd2O7dHXz M3rQPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEC2NFE3Lz5{NUSwOlAyPjxxYU6=
RBMECs MUjGeY5kfGmxbjDBd5NigQ>? M2GzZVXDqM7:TR?= NH;LZWcyKGh? NVTvR3My[myxY3vzJJRp\SCjY4TpckBkgXSxc3vlcIV1d25icnXhdpJidmenbXXueEB{\WWwIIfpeIghTU2DUD3JTUB1emWjdH3lcpTDqA>? Mo\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MU[2OVEoRjJ3NEG2OlUyRC:jPh?=
RBMECs MmTlSpVv[3Srb36gRZN{[Xl? NHTW[nE2yqEQvF2= NYntco1yOSCq NYjZN5oz[myxY3vzJJRp\SCHTVHQMWlKNWmwZIXj[YQh[2ijbnflJIlvKE2OQzDwbI9{eGixconsZZRqd25? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRzNk[1NUc,OjV2MU[2OVE9N2F-
RBMECs MW\GeY5kfGmxbjDBd5NigQ>? NF7hT2I2yqEQvF2= MYOxJIg> M1XBNJJmfmW{c3XzJJRp\SClaHHu[4V{KGmwIGrPMVEh\Gm|dILpZpV1cW:wIIPl[Y4hf2m2aDDFUWFRNUmLIITy[YF1dWWwdB?= NFG1eWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSxOlY2OSd-MkW0NVY3PTF:L3G+
RBMECs MX3GeY5kfGmxbjDBd5NigQ>? M3XoZVXDqM7:TR?= Mlq3NUBp NVTBfpZVcW6qaXLpeJMhfGinIHTlZ5Jm[XOnZDDv[kBidW:3boSgc4YhYk9vMTDpckBOTnNiaX7keYNm\CCkeTDFUWFRNUmL NFfVZWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSxOlY2OSd-MkW0NVY3PTF:L3G+
RBMECs NWDyToY6TnWwY4Tpc44hSXO|YYm= NIPwcoQ2yqEQvF2= Mn;uNUBp NWrnZlRbeHKndnXueJMhfGinIHnuZ5Jm[XOnIHnuJGhTWCCobIX4JIFkem:|czD0bIUhSlSEIHnu[JVk\WRiYomgJGVOSVBvSVm= NF7UV5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSxOlY2OSd-MkW0NVY3PTF:L3G+
RBMECs NF[wU3BHfW6ldHnvckBCe3OjeR?= NH;4N242yqEQvF2= NVPocmhVOSCq MX3wdoV3\W62czD0bIUhTU2DUD3JTU1qdmS3Y3XkJHRGTVJidnHseYUh\GWlcnXhd4U> NI[5Z|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSxOlY2OSd-MkW0NVY3PTF:L3G+
RBMECs MV3GeY5kfGmxbjDBd5NigQ>? NHWxW|I2yqEQvF2= NWm5eI5UOSCq MUjicI9kc3NidHjlJIFkfGm4YYTpc44hd2ZiUnjvRU9TV0ONIHnu[JVk\WRiYomgSW1CWC2LSR?= NET6NXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSxOlY2OSd-MkW0NVY3PTF:L3G+
RBMECs M{DG[2Z2dmO2aX;uJGF{e2G7 MXOwMlA2NzBwNT:1JO69VQ>? MVWwMlI2KGh? NXzQW4Q{cW6lcnXhd4V{KGODTWCgZ49v[2WwdILheIlwdg>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRzNk[1NUc,OjV2MU[2OVE9N2F-
ThGCs  NYi0d29[TnWwY4Tpc44hSXO|YYm= NF7VfYcyOOLCit88US=> MmnzN{Bp MkDkbY5pcWKrdIOgeIhmKGWoZnXjeEBw\iCKMl:yJI9vKEWGTkKgcXJPSQ>? M{\sdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOzNFI4Lz5{NUSzN|AzPzxxYU6=
ThGCs  NYTDS5R4TnWwY4Tpc44hSXO|YYm= MnvhNVDjiIsQvF2= MVq05qCLcA>? MlnNbY5kemWjc3XzJGNwS2x{LXnu[JVk\WRiRVTONuKh\2WwZTDlfJBz\XO|aX;u NG\4Wnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzN|AzPyd-MkW0N|MxOjd:L3G+
ThGCs  MnrnSpVv[3Srb36gRZN{[Xl? MVGxNQKBks7:TR?= MYe05qCLcA>? MlXSZZVodWWwdIOgTGlHOUFibHX2[Yx{KHSqYYSge4Vz\SC|dHnteYxifGWmIHL5JGNwS2x{ NWDs[pJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|MxOjdpPkK1OFM{ODJ5PD;hQi=>
LNCaP  NYLvSlVJTnWwY4Tpc44hSXO|YYm= M16zS|ExyqEQvF2= MoDINVIhcMLi NVWy[2pWTE2VTx?= M3OzeIlv\HWlZYOgZUBlemGvYYTpZ{BqdmO{ZXHz[UBw\iCFUlXCNUBi[3Srdnn0fS=> Mn6zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NEiwPVkoRjJ3NUS4NFk6RC:jPh?=
BeWo M3SxRWZ2dmO2aX;uJGF{e2G7 MVSyNOKhyrWP NGfq[G81QMLiaB?= Ml\uSG1UVw>? NFLVepNqdmO{ZXHz[ZMhfGinIHHkbIV{cW:wIH;mJHRJWC1zIH3vco9kgXSncx?= M4HLO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[2O|QxLz5{NUW2Olc1ODxxYU6=
BeWo NETsN2tHfW6ldHnvckBCe3OjeR?= MVyyNOKhyrWP NX61[oV{PDkEoHi= Ml;aSG1UVw>? MX3pcoNz\WG|ZYOgeIhmKGSrZn\ldoVvfGmjdHnvckBw\iCEZWfvJINmdGy| NIPjZ489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2Olc1OCd-MkW1OlY4PDB:L3G+
OCI-Ly18  M13jXWZ2dmO2aX;uJGF{e2G7 NXXTT|dSPDEEoN88US=> MkPGNeKhcMLi M2[3V2ROW09? MVPpcoR2[2W|IITo[UBqdmO{ZX3lcpQhd2ZiY1HNVEBkd26lZX70doF1cW:wcx?= MoPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3N{[yNlAoRjJ3NUe2NlIxRC:jPh?=
OCI-Ly1  MXfGeY5kfGmxbjDBd5NigQ>? MX:0NOKh|ryP NF\MVo0yyqCqwrC= Ml7CSG1UVw>? M13sOolv\HWlZYOgeIhmKGmwY4LlcYVvfCCxZjDjRW1RKGOxbnPlcpRz[XSrb37z NFHYeZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW3OlIzOCd-MkW1O|YzOjB:L3G+
3T3-L1 NIXtbnpHfW6ldHnvckBCe3OjeR?= MVuyMlUwPSEQvF2= MXiyOEBpyqB? MV3zbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCDVFfMJJBzd3SnaX6g[ZhxemW|c3nvckBifCCjbHyg[I9{\XNidHXzeIVl NUS2UZhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVA2QTdpPkK1OVkxPTl5PD;hQi=>
HEK293  NIDjVJhHfW6ldHnvckBCe3OjeR?= MVi15qCKyrWP NXHkVGlFOzBibXnu Mof0bY5kemWjc3XzJINCVVBibHX2[Yx{ NFr5Zmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5NVkxQCd-MkW1PVE6ODh:L3G+
hADSCs M3fpc2Z2dmO2aX;uJGF{e2G7 M37UZ|XjiIoEtV2= MkCwN|AhdWmw MU\pcoNz\WG|ZYOgZ2FOWCCuZY\lcJM> NX7zToQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVE6ODhpPkK1OVkyQTB6PD;hQi=>
SH-SY5Y  NXK5VYl7TnWwY4Tpc44hSXO|YYm= M3vxSlExyqEQvF2= Mo\vNeKhcMLi NILGcmdqdmO{ZXHz[ZMhSUeFMTDtVm5CKGyndnXs MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7N{SzN{c,OjV3OUe0N|M9N2F-
SH-SY5Y  MXXGeY5kfGmxbjDBd5NigQ>? M4\jXlExyqEQvF2= NIj1OJIyyqCqwrC= MnzPbY5kemWjc3XzJGxWSyCjY4Tpeol1gQ>? MmLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUe0N|MoRjJ3NUm3OFM{RC:jPh?=
UACC-647  M{H3SmZ2dmO2aX;uJGF{e2G7 NUnWU4FIOTEEoN88US=> NIX2[FAyPSCvaX6= MmPISG1UVw>? M{XZWYlv[3KnYYPld{BmTUZ{IIDoc5NxcG:{eXzheIlwdiCuZY\lcJPDqA>? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyM{CyOUc,OjV5MEOwNlU9N2F-
UACC-647  NYGzWWhJTnWwY4Tpc44hSXO|YYm= MWCxNOKh|ryP NXi5TY5UOTVibXnu MWDEUXNQ NFniPJVqdmirYnn0d{BGWkticHjvd5Bpd3K7bHH0bY9v NYq2PIdoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFMxOjVpPkK1O|A{ODJ3PD;hQi=>
UACC-647  M2n1N2Z2dmO2aX;uJGF{e2G7 Ml\0SG1UVw>? NIL0enZt\WGmczD0c{BiKHKrc3WgbY4h[0GPUDDs[ZZmdHNiKFXDOVDDqD4EoEKwMlM6yqEQvF2p MojYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOwNlUoRjJ3N{CzNFI2RC:jPh?=
SC NIPyTm1HfW6ldHnvckBCe3OjeR?= NWjJcZN1OC53IN88US=> M2rRZ|czKGh? MlfKbY5kemWjc3XzJIJwfGhiS4LvfE0zOCCjbnSgU|Eh\XiycnXzd4lwdiCrbjDhfI9vNXKnbHH0[YQhW0O|IHL1eEBwdmy7IFvyc5guOjEEoB?= MorSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEW4O|QoRjJ3N{C1PFc1RC:jPh?=
SC NIjwZ2JHfW6ldHnvckBCe3OjeR?= MljQNE42KM7:TR?= NHzkTFUzPCCq MkTpcYlucWOtczD0bIUh\W[oZXP0JI9nKGODTWCgZY5idG:pczDvckBQOSCjbnSgUWJRKGW6cILld5Nqd25? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyNUi3OEc,OjV5MEW4O|Q9N2F-
oocytes NUP3PVg2TnWwY4Tpc44hSXO|YYm= MkDkOUDPxE1? NH[3V3AzPCCq M2L1PIF1fGWwdXH0[ZMhemhvaX7zeYxqdiCjY4Tpc44hd25ib3;jfZRmKEeYQlSgd4lodmmoaXPhcpRtgcLi M3HDNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{C3PFU1Lz5{NUewO|g2PDxxYU6=
BeWo M2e2c2Z2dmO2aX;uJGF{e2G7 Ml7QNVDjiIsQvF5CpC=> NV;OVpREPzJiaB?= M4XR[WROW09? NYDteYo5dWWmaXH0[ZMhSmWZbzDj[YxtKGSrZn\ldoVvfGmjdHnvci=> NIXkeoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUexN|QzPSd-MkW3NVM1OjV:L3G+
GH3 NXyxdFJtTnWwY4Tpc44hSXO|YYm= MkHBNeKh|ryP NFvIWXY3NWh? MnjBbY5lfWOnczDQVmwh[W6mIFLtZYwyNCCkdYSgco91KEOub3PrMEBuWk6DIHX4dJJme3Orb36= MmC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkewNVgoRjJ3N{K3NFE5RC:jPh?=
GH3 NEC0VJhHfW6ldHnvckBCe3OjeR?= MkTjNeKh|ryP NGfWe|I3NWh? NYDSN443[XS2ZX71ZZRmeyC2aHWgZ49zemWuYYTpc44h[mW2d3XlckBRWkxiYX7kJGJu[WxzIHX4dJJme3Orb36= M4frO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{K3NFE5Lz5{NUeyO|AyQDxxYU6=
PC12 NHXUXGlHfW6ldHnvckBCe3OjeR?= NFH0OJIzPcLizszN NFr4RZE1QCCq NVXSW2w{[WO2aY\heIV{KGODTWC= Mo[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkmzNFUoRjJ3N{[5N|A2RC:jPh?=
BAECs NVTufIxJTnWwY4Tpc44hSXO|YYm= M2LlclI2KM7:TR?= NHqyNIMzPCCq M1HkZoVvcGGwY3XzJJRp\SCjY4TpeoF1cW:wIH;mJHBRSVMQsTDifUA2KM7:TTDy[ZN3\XKjdILvcEwhXDSKUzygc5IhPC2SQWC= NX7r[JRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PVg5OjZpPkK1O|k5QDJ4PD;hQi=>
GLUTag  NWnWN3JWTnWwY4Tpc44hSXO|YYm= NF3EZlQyOOLCidM1US=> MU[0JIg> MXzpcoNz\WG|ZYOgeIhmKHCFUlXCJIxmfmWuczD3bZRpKHSqZTDJRm1Z MnjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6M{K2N|EoRjJ3OEOyOlMyRC:jPh?=
GLUTag  MlXPSpVv[3Srb36gRZN{[Xl? NFu5UocyOOLCidM1US=> MXGwM|IwPCCq NGHWVJd{fGmvdXzheIV{KEeOUD2xJJNm[3KndHnvckBkd3S{ZXH0[YQhf2m2aDDJRm1Z NELPWWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUizNlY{OSd-MkW4N|I3OzF:L3G+
PBMC NV;rTIpjTnWwY4Tpc44hSXO|YYm= MXq1NOKh|ryP MXSyOOKhcMLi NYXrU5NmcW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDz[YNz\XSrb36gc4YhXE6IIHnu[JVk\WRiYomgeIhmKESSRR?= NHvXSpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2OlA4QSd-MkW4OlYxPzl:L3G+
H295R  MXfGeY5kfGmxbjDBd5NigQ>? NUfXNXlbOTEEoN88US=> M1LuU|Q5yqCq MVLpcoNz\WG|ZYOgd5Rmem:rZDDt[ZRi[m:uaYTld{BqdiC2aHWgZY5lem:pZX6sJI1qdmW{YXzvMUBidmRiZ3z1Z49kd3K2aXPvbYQheGG2aIfhfZM> M3nUV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[5OVU3Lz5{NUi2PVU2PjxxYU6=
3T3-L1 preadipocytes Ml\uSpVv[3Srb36gRZN{[Xl? NUK5PJhmOTBizszNxsA> NXzKNodtOTJiaB?= MVvpcoR2[2W|IFPSSWIheGixc4Doc5J6dGG2aX;uJIFv\CCFL1XCVO6zKGW6cILld5Nqd25? Mmj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7MkiwOVgoRjJ3OUK4NFU5RC:jPh?=
PCCL3 MlHsSpVv[3Srb36gRZN{[Xl? M3j5[VExKML3TR?= M{O4RVI1KGh? MmHR[Y5p[W6lZYOgSJVQgDJicILvcY91\XJidILhcpNkemmydHnvckBi[3Srdnn0feKh6oDN M2W1eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OU[wPVU3Lz5{NUm2NFk2PjxxYU6=
SCG NELh[YVHfW6ldHnvckBCe3OjeR?= NH;HNWsyODBizszNxsA> MkjNSG1UVw>? NFm3Tm5z\WS3Y3XzJJRp\SCneHPpeIFjcWyrdImgc4YhW0OJIH7leZJwdnN? NYLVTINMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OlIyOzJpPkK1PVYzOTN{PD;hQi=>
HEK-293 MnnNSpVv[3Srb36gRZN{[Xl? NHzXN4o{PSEQvF5CpC=> NWHkeno2TE2VTx?= M4fVO4lv\HWlZYOgZUBkd26|cHnjeY92eyEkgKzpcoFkfGm4YYTpc47jiJ1ib3[geIhmKEu4Mj6xJIN2enKnboS= MljLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkKxN|IoRjJ3OU[yNVMzRC:jPh?=
SCG NUXVXGN7TnWwY4Tpc44hSXO|YYm= MXyyNEDPxE4EoB?= NYTnVphiTE2VTx?= M4DhcpJmfmW{c3nicJkhe3WycILld5NmeyCLS2[ge4l1cCCjIFnDOVAhd2ZiMkSuOEDPxE1? NUXtUFhtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OlIyOzJpPkK1PVYzOTN{PD;hQi=>
PC-3 NFzvdI9E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXW4SWlWPDBiwsXN M4H3d|I1NzR6L{eyJIg> M1fJZ2ROW09? NXrXSHNb\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JJRqdWViZHXw[Y5l\W62bIm= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{M{izOkc,OjZyMkO4N|Y9N2F-
PC-3 NUC0UZJKTnWwY4Tpc44hSXO|YYm= MojwOFAhyrWP M2X3flIhcA>? Mny0SG1UVw>? M3H5TIxm[WS|IITvJHBROkFiYXP0bZZifGmxbh?= M4rtOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEKzPFM3Lz5{NkCyN|g{PjxxYU6=
SH-SY5Y NGXWVXNHfW6ldHnvckBCe3OjeR?= MkOxN|Ah|ryP NXroPHE3OzBibXnu NH3T[mdFVVOR MYTzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyC2aHWgZYN1cX[jdHnvckBw\iCSS1G= M{X0N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEK1NVM4Lz5{NkCyOVE{PzxxYU6=
EndoC-βH1 NHS5bpBHfW6ldHnvckBCe3OjeR?= MYW1xsDPxE1? M3PDOFEhcA>? M3jxXIxm[WS|IITvJIEhe3S{b37nJINCVVBiaX7jdoVie2V? NVnqNFJKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNlg2PjJpPkK2NFI5PTZ{PD;hQi=>
EndoC-βH1 M33KO2Z2dmO2aX;uJGF{e2G7 Mm\WOeKh|ryP M4iyRVEhcA>? M3n0V5BwfGWwdHnheIV{KGeudXPvd4UucW6mdXPl[EBqdnO3bHnuJJNm[3KndHnvckBqdiC2aHWgdJJme2WwY3Wgc4Yh\2y3Y3;z[S=> NHrFe5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCyPFU3Oid-Mk[wNlg2PjJ:L3G+
RBMECs NYPpNIlGTnWwY4Tpc44hSXO|YYm= M2\5blXDqM7:TR?= MnfiNUBp NIfKPJVqdmirYnn0d{BGVUGSLVnJMYlv\HWlZXSgbY5i[3SrdnH0bY9vKG:oIGLhdFHDqA>? Ml65QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyNES2OlMoRjJ4MES0OlY{RC:jPh?=
AML-12  MWHGeY5kfGmxbjDBd5NigQ>? MnzVNlAh|ryP NF;RbHA{KGh? NIfxT2tqdmS3Y3XzJJRp\SCmZYDoc5NxcG:{eXzheIlwdiCxZjDDVnREOsLi M{LZcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES4PVg2Lz5{NkC0PFk5PTxxYU6=
AML-12  M1uySWZ2dmO2aX;uJGF{e2G7 M4\UZ|IxKM7:TR?= NFfUXnc{KGh? NGD0S252eC2{ZXf1cIF1\XQEoGDnZ|FiNMLiUHXwZ4stKGGwZNMgS|Zx[8LibWLORUBt\X[nbIO= Mn3pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyNEi5PFUoRjJ4MES4PVg2RC:jPh?=
AML-12  MWHGeY5kfGmxbjDBd5NigQ>? MVqyNEDPxE1? MoDXNU05KGh? MY\pcoNz\WG|ZYOg[4x2[2:|ZTDwdo9lfWO2aX;u M{\DXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES4PVg2Lz5{NkC0PFk5PTxxYU6=
AML-12  NILIXXBHfW6ldHnvckBCe3OjeR?= Ml6zNlAh|ryP MW[zJIg> MW\1dJJm\3WuYYTld{B1cGVicHjvd5Bpd3K7bHH0bY9vKGyndnXsd{BifCCWaIKtOFEyKGGwZDDT[ZIuPDl| NGPwSoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC0PFk5PSd-Mk[wOFg6QDV:L3G+
Caco-2  NWfBVYs2TnWwY4Tpc44hSXO|YYm= MXmwMlEwOS9zMDFOwG0> MlvQNlQhcA>? NH7IZ2ZqdmO{ZXHz[ZMhVVKSMjDwdo91\WmwIHzleoVt M3SzPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES5NVAzLz5{NkC0PVExOjxxYU6=
Caco-2  MXPGeY5kfGmxbjDBd5NigQ>? NEj5UGUxNjFxMT:xNEDPxE1? M3[3PVIxyqCvaX6= M2D2Tolv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDpcoNz\WG|ZTDpckBqdnS{YXPlcIx2dGG{IHPBUXAhdGW4ZXzz NXm1coV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wOFkyODJpPkK2NFQ6OTB{PD;hQi=>
bovine oocytes MlfpSpVv[3Srb36gRZN{[Xl? NIewNWoyODEEoN88US=> Ml;5NVLDqGh? M2DEbIlvcGmkaYTzJJRp\SCnZn\lZ5Qhd2ZiTmDQRUBidmRxb4KgUnBRSyC2bzDzeIlufWyjdHWgdoV{fW2ydHnvckBw\iCvZXnvd4l{ NGPVfZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC1NVYyOSd-Mk[wOVE3OTF:L3G+
BeWo  M2jBUWZ2dmO2aX;uJGF{e2G7 NVf6SmpLOjYkgJpOwG0> MYKyOE81QC95MjDo MmPpcIVi\HNidH:gZY4hcW6lcnXhd4UhcW5idHjlJIV5eHKnc4Ppc44hd2Zib4To[ZIh\nW|aX;uJI1iemuncoO= NHWyOJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC1N|U1QSd-Mk[wOVM2PDl:L3G+
Spinal cords  NVrYbXN{TnWwY4Tpc44hSXO|YYm= NEfWeZgyyqEQvF2= NVPnUYREOzBibXnu NYC5fGZIe3SrbYXsZZRmeyClQV3QJIxmfmWucx?= M{n0ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUK2PVI3Lz5{NkGyOlkzPjxxYU6=
MDCK  NWPE[IpVTnWwY4Tpc44hSXO|YYm= MVOxNEDDvU1? M3fHXVI1KGh? NUHRZWplTE2VTx?= NWTFcndFcW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDlfJBz\XO|aX;uJI9nKE[QIHPheZNm\CCkeTDUS2Yu|rJz Ml34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{MEKzOVIoRjJ4MkCyN|UzRC:jPh?=
MDCK  MnfOSpVv[3Srb36gRZN{[Xl? M4nxRVExKML3TR?= MXKyOEBp MVvEUXNQ NXjJSWFMfXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiVFfGMe6zOSCjbnSgR3RITsLi NGOw[Gc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkKwNlM2Oid-Mk[yNFI{PTJ:L3G+
RPMI 8226 NV;1PYxmS2WubDDWbYFjcWyrdImgRZN{[Xl? M4OzNFAuOTByIN88US=> Mn7HO|LjiImq NE[yOlRqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> Mmf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|ME[2NlQoRjJ4M{C2OlI1RC:jPh?=
H929 NWS0UVVqS2WubDDWbYFjcWyrdImgRZN{[Xl? M2[xfFAuOTByIN88US=> NYnXV|F{PzMkgJno NF3G[JZqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NIfFWXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOwOlYzPCd-Mk[zNFY3OjR:L3G+
U266 Mn;6R4VtdCCYaXHibYxqfHliQYPzZZk> Mk\QNE0yODBizszN MX63NwKBkWh? MmDsbY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= Mn7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|ME[2NlQoRjJ4M{C2OlI1RC:jPh?=
OPM-2 NX;RXlZtS2WubDDWbYFjcWyrdImgRZN{[Xl? Mo\QNE0yODBizszN MXG3NwKBkWh? NHnO[4xqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> NE\ZOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOwOlYzPCd-Mk[zNFY3OjR:L3G+
INA-6 MWXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MV[wMVExOCEQvF2= NEnnVm04OuLCiXi= MkjPbY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= M4WyNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{C2OlI1Lz5{NkOwOlYzPDxxYU6=
RBMECs  NHzjfWJHfW6ldHnvckBCe3OjeR?= NI\BflU2yqEQvF2= MlnnNeKhcA>? NWDK[Isz[myxY3vzJJRp\SCUYXOxJIlv[WO2aY\heIlwdiCrbnT1Z4VlKGK7IFXNRXAuUUl? NFniVlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO1PFA{QSd-Mk[zOVgxOzl:L3G+
Mo-DCs MVPGeY5kfGmxbjDBd5NigQ>? NYDpbmtOPTBizszN NEfyTpkzPOLCiXi= NVX4PY1WeHKxbX;0[ZMhUUxvMkOgdJJw\HWldHnvckBqdiC2aHWgd5Vx\XKwYYThcpQhd2Zieontc5NidiC|dHnteYxifGWmIF3vMWREe8Li NFfocpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxNlk1QCd-Mk[0NVI6PDh:L3G+
HEK293 MWXGeY5kfGmxbjDBd5NigQ>? NIf6W3gyOCEQvF2= MlLqOkBp NHHucVBqdmO{ZXHz[ZMheGixc4Doc5J6dGG2aX;uJI9nKG:4ZYLlfJBz\XO|ZXSgT2xJVDNiYYSgV|Q{Ow>? NUXU[HpERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0N|U1QThpPkK2OFM2PDl6PD;hQi=>
ASK NVLLfYJ5TnWwY4Tpc44h[XO|YYm= Ml\tNUBpeg>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NkSxK|4zQDR7NkSxQE9iRg>?
Vero E6 NEPVRlNCdnSrdnnyZYwh[XO|YYm= NW\nOXdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlM2OzlpPkG3OlY{PTN7PD;hQi=>
epithelial cells MoHuSpVv[3Srb36gZZN{[Xl? M3XhWFEhfU1? M1XyVFQtKDZuIHHu[EA5KGSjeYO= NIHpTlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
HepG2 (DPX-2) Mn;JSpVv[3Srb36gZZN{[Xl? NFTiXWkzPCCqcoO= M{nGNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOU[2NFQ{Lz5{MEm2OlA1OzxxYU6=
HepG2 NYf2VIxWTnWwY4Tpc44h[XO|YYm= MmXRNlQhcHK| MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl4NkC0N{c,OjB7Nk[wOFM9N2F-
HepG2 (DPX-2) NIXIPIhHfW6ldHnvckBie3OjeR?= MmHRNlQhcHK| Mn7LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7Nk[wOFMoRjJyOU[2NFQ{RC:jPh?=
HEK293T MUnGeY5kfGmxbjDhd5NigQ>? NYnmTW5xOTBidV2= NVLacVhPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPFc{OjVpPkK0N|g4OzJ3PD;hQi=>
HEK293 MVXGeY5kfGmxbjDhd5NigQ>? NIe5ZpUyOCC3TR?= M{nzSlE3KGi{cx?= M3zyT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEO1OVEzLz5{NkSzOVUyOjxxYU6=
MCF7 MoPwR5l1d3SxeHnjbZR6KGG|c3H5 NXm0R4Z4PDhiaILz MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh|OE[5Nkc,Ojh6M{i2PVI9N2F-
HEK293 MlPQSpVv[3Srb36gZZN{[Xl? MYqzNEBucW6| NG\Hemg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MECwOlE4Pid-M{CwNFYyPzZ:L3G+
Assay
Methods Test Index PMID
Western blot cleaved caspase-3 / caspase-3 cleaved caspase-9 / caspase-9 β-catenin c-myc / Cyclin D1 pS6K1 / S6K1 / pCREB / CREB p-JNK / JNK / P-p38 / p38 30863177 31112131 20070884
Immunofluorescence 5hmC Fe(II) CYP17A1 / CYP21A2 29239726 25334044
Growth inhibition assay Cell viability 30863177

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度(25°C)

体外

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 410.5
化学式

C22H34O7

CAS号 66575-29-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04254705 Unknown status Procedure: Rectal Biopsy Cystic Fibrosis Universitaire Ziekenhuizen Leuven|Vertex Pharmaceuticals Incorporated|KU Leuven|University of Lisbon March 1 2020 Not Applicable
NCT02807415 Completed Drug: Lumacaftor plus Ivacaftor Cystic Fibrosis Hannover Medical School|Heidelberg University|University of Giessen June 1 2016 --
NCT02586883 Completed Other: bronchial ddp test Idiopathic Dilation of the Bronchi Assistance Publique - Hôpitaux de Paris March 29 2016 Not Applicable
NCT03652090 Completed Procedure: cell sampling Cystic Fibrosis Institut National de la Santé Et de la Recherche Médicale France|ABCF2 September 1 2010 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们