(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2351.11 现货
RMB 2227.39 现货
RMB 4667.45 现货
RMB 11220.4 现货
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客户使用Selleck生产的(+)-JQ1发表文献281篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MlnzR4VtdCCYaXHibYxqfHliQYPzZZk> MnPMNlUxNzVyMD:xNFAxKG6P MnfQNlQwPDhxN{KgbC=> M1PRfmROW09? MnTCbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> MoLUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
BCPAP MVfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NYDTOpZUOjVyL{WwNE8yODByIH7N NH\PWY8zPC92OD:3NkBp NI\EcmNFVVOR M1L6bIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> MkPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
K1  MkXKR4VtdCCFeXPs[UBCe3OjeR?= NWrkRXZGOjVyL{WwNE8yODByIH7N MlL6O|IhcA>? M3;2T2ROW09? NGjKRZFienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
BCPAP NEHIU4ZE\WyuIFP5Z4xmKEG|c3H5 M1vRXFI2OC93MECvNVAxOCCwTR?= NUXWeJl1PzJiaB?= M1TPdGROW09? M2WwUIFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= Mlq2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEe4PFEoRjJ4N{C3PFgyRC:jPh?=
Hep3B MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfKNE0yOCEQvF2= NYjmfIJEPSCm NV7qU|JETE2VTx?= M4rhd2lEPTB;MD6wPEDPxE1? NYG2fYxCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HCCLM3 M1XJT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzybpdNOC1zMDFOwG0> MUS1JIQ> Mn30SG1UVw>? NF65VmRKSzVyPUCuNVQh|ryP NEXaNWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HuH7 NYf5[4k2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nsfVAuOTBizszN M4\VcVUh\A>? NUPrfpVFTE2VTx?= MVfJR|UxRTBwMkGg{txO M{\yTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HepG2 NI\pSJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnFb5dTOC1zMDFOwG0> NIfYXFk2KGR? M1LCNGROW09? NVvQSVN{UUN3ME2wMlM1KM7:TR?= M1GyV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
SMMC7721 M4[wWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL2NY1KOC1zMDFOwG0> NHrTVm02KGR? NHPSZWFFVVOR NUCzVVJyUUN3ME2wMlQyKM7:TR?= NWPyNnBPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
BEL7402 M4TMXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMVExKM7:TR?= MXS1JIQ> M3G5bGROW09? MkHjTWM2OD1yLkS3JO69VQ>? M1SxeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
MHCC97H M4Xa[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXTNE0yOCEQvF2= MUW1JIQ> M3KxZ2ROW09? NYq3OJE3UUN3ME2wMlQyKM7:TR?= Moj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
Hep3B MnToR4VtdCCFeXPs[UBCe3OjeR?= MlTNNE4yNzBwNT:yMlUh|ryP M2fibFQ5KGh? MVjEUXNQ M1TRXIxm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 NFTzdVJE\WyuIFP5Z4xmKEG|c3H5 M1THfVAvOS9yLkWvNk42KM7:TR?= M1nzfVQ5KGh? NFnlcIZFVVOR NXL6dWo3dGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
Hep3B M37pbGFxd3C2b4Ppd{BCe3OjeR?= MWGwMlEwOC53L{KuOUDPxE1? NEHEeWY1QCCq NFK1VGtFVVOR NYi2UYJJ[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NIX6fY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 NWfIcFlzSXCxcITvd4l{KEG|c3H5 NUW5VGdxOC5zL{CuOU8zNjVizszN NEjHO2c1QCCq MkLDSG1UVw>? NGrtXFFi[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj NXzkfoJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
A549 NEW0bZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFy1[44xNjFvMUCg{txO MUm3NkBp M4TxU4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz Mn7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 M2qxU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXReJoxNjFvMUCg{txO M3rnOlczKGh? NY[1WVhmcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MnvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 NWe0NYczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLRdppbOC5zLUGwJO69VQ>? MVi3NkBp MX3pcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MoG2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
A549 Mn;lSpVv[3Srb36gRZN{[Xl? NWLGXXd2OS9{LkWvOUDPxE1? NU\0UnZDOTJiaB?= MWpCpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H1299 M2fQZmZ2dmO2aX;uJGF{e2G7 MoHiNU8zNjVxNTFOwG0> M37n[|EzKGh? NYrTVFI{yqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H157 M3nUWmZ2dmO2aX;uJGF{e2G7 MkHzNU8zNjVxNTFOwG0> M1;x[lEzKGh? NET4cXRl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= M1jo[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MlfnSpVv[3Srb36gRZN{[Xl? Mk\pNU8zNjVxNTFOwG0> NFPLWIQyOiCq MoGx[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w Mn3HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
C8161 M{W1RmNmdGxiVnnhZoltcXS7IFHzd4F6 Mn\UNE0zKM7:TR?= NULTOJBMPCCm M4nT[GROW09? NX72TGo5\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel285 NVLhdXYyS2WubDDWbYFjcWyrdImgRZN{[Xl? MWewMVIh|ryP MYe0JIQ> MVfEUXNQ MWTk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel290 NEW2fnRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NGnkS3AxNTJizszN MkHwOEBl MYfEUXNQ NGX2NoFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 MWLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M3fSTFAuOiEQvF2= Mn3XOEBl Mm\BSG1UVw>? NXGxPIp{\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1.3 NVHwU2F[S2WubDDWbYFjcWyrdImgRZN{[Xl? M{LDSFAuOiEQvF2= MnPMOEBl MWDEUXNQ NUnGWXJ3\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel202 MWjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NYXNPYRwOC1{IN88US=> MXq0JIQ> MV7EUXNQ MlLn[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M{G4WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel270 NWD0[FR2S2WubDDWbYFjcWyrdImgRZN{[Xl? MUCwMVIh|ryP NI\5SVA1KGR? NYLZboFzTE2VTx?= MofT[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NGrHU2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1 NIHSO3RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUGwMVIh|ryP MlLrOEBl NV;GfFNHTE2VTx?= MXrk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NEDIbIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 MVzBdI9xfG:|aYOgRZN{[Xl? NH;WSWI2ODBibl2= M3;NV|Q5KGh? NHLDXnlFVVOR M4HtUIlv\HWlZYOgZZBweHSxc3nz MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1.3 MnzTRZBweHSxc3nzJGF{e2G7 MY[1NFAhdk1? M2PtNlQ5KGh? M2Tv[GROW09? NXL1[nV4cW6mdXPld{BieG:ydH;zbZM> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 NWXOZnV2S2WubDDDfYNt\SCDc4PhfS=> Mnq5OVAxKG6P NYrDO4RXOjRxNEivO|IhcA>? MmniSG1UVw>? NF\nOI5qdmS3Y3XzJJRp\SClZXzsJIFk[3WvdXzheIlwdiCjdDDzeYIuTzIEoB?= NXPPPYZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
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Rosetta2 DE3 M4i3NmZ2dmO2aX;uJIF{e2G7 NFPJSo5M\CB;IECuNFA3OiEQvF2= NITrUlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
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Rosetta2 DE3 MYXGeY5kfGmxbjDhd5NigQ>? NULFPW1oU2RiPTCwMlAxPjdizszN NYfodoZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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Rosetta2 DE3 MV\GcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NYPyRmdFOzBibXnudy=> MUTLbUA:KDBwMEGwO{DPxE1? NVPsRnQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NH73TIxHfW6ldHnvckBie3OjeR?= Mn7ZT4QhRSByLkCxNVch|ryP M3jjWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
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Rosetta2 DE3 NEXGUVVHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 MXmzNEBucW6| M1nhPWtqKD1iMD6wNVIh|ryP NXG2SJRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
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Rosetta2 DE3 NV\TTWpWTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= Ml;LN|AhdWmwcx?= NUPFXYM3U2liPTCwMlAyOjVizszN MnG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 M2jRPGZ2dmO2aX;uJIF{e2G7 NGnyNmdM\CB;IECuNFEzQCEQvF2= NWnJSnA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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Rosetta2 DE3 NFnmdGpHfW6ldHnvckBie3OjeR?= MYLL[EA:KDBwMEG0O{DPxE1? NY\Pd2l2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 MXTGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NEeyV5Q{OCCvaX7z NYPvUnN5U2liPTCwMlAyPDlizszN Ml;wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
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MV4-11 NIfrXWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3HuV|czKGh? NUDMPFREUUN3MDC9JFAvODJ|IN88US=> MkPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUm0NlgoRjJ3NUW5OFI5RC:jPh?=
MV4-11 M{\CcWN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\RTm41KGSjeYO= NF\kTVhKSzVyIE2gNE4xOjRizszN MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MV4-11 NVjVN5p3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIXScXI1KGSjeYO= MnnHTWM2OCB;IECuNFI1KM7:TR?= NXzQfYxURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
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Rosetta2 DE3 M2njcWZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? MVGzNEBucW6| MoTxTWM2OCB;IECuNFQzOiEQvF2= M1TPTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
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MV4-11 M{TCXGN6fG:2b4jpZ4l1gSCjc4PhfS=> NUDr[I1XOjRiaB?= M2rmXWdKPTBiPTCwMlA5KM7:TR?= M{XofVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUmxN|Y{Lz5{NkG5NVM3OzxxYU6=
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C4-2B M2\tO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1qyfVEzKGh? NYjJOWF3UUN3MDC9JFAvOTlizszN MlTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
Vero E6 NHfSbHNCdnSrdnnyZYwh[WO2aY\peJkh[XO|YYm= NGeyNGY1QCCq NWfVSYZjUUN3MDC9JFAvOTl{N{Wg{txO NVTLVWhERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KzOVM5PTlpPkOyN|U{QDV7PD;hQi=>
MCF7 MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MonRNVIhcA>? M{TlXmlEPTBiPTCwMlIh|ryP NVPGbWFPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MV4-11 NHmxRnFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHfTc3NKSzVyIE2gNE4zPCEQvF2= M3X5NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUSyO|UyLz5{N{G0Nlc2OTxxYU6=
MV4-11 NHXNSFZEgXSxdH;4bYNqfHliYYPzZZk> MVm3NkBp MVjJR|UxKD1iMD6yOFIh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVzN{CxNUc,OjN3MUewNVE9N2F-
MX1 NGDZfWtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NX;WO45kPzJiaB?= NUHod5FUTUN3MDC9JFAvOjV2IN88US=> M13lTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUS5OVIyLz5{OEm0PVUzOTxxYU6=
HT-29 NIfzVZlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NX7id4ZXPzJiaB?= NFrtRVRKSzVyIE2gNE4zQCEQvF2= Mn[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
HFL1 NFXjV4pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUDMXZJtOTJiaB?= MX;JR|UxKD1iMD6yPUDPxE1? NVP5TmpZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
MDA-MB-231 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkTEN{Bl[Xm| NGmyW2RISzVyIE2gNE4{KM7:TR?= NW\YfI9pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
MV4-11 MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MXK0PEBp M1TnVmVEPTBiPTCwMlM{OTF|IN88US=> M{m3TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
K562 NILvRVZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYXJR|UxKD1iMD62OEDPxE1? MnLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNEK3OVEoRjJ5MUSyO|UyRC:jPh?=
HL60 NXfpT5ZwSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIriXXk1QCCq MUXFR|UxKD1iMD63OFE{OSEQvF2= M3\TZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
NCI-H1975 M1X2VWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MV[xNkBp NWrOVmo5UUN3MDC9JFEvOjNizszN NGLhT3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
SAE MX;GeY5kfGmxbjDhd5NigQ>? Ml7LOEBp NF6ycXZKSzVyIE2gNU4{QCEQvF2= NFrpVWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
SAE M{D2U2Z2dmO2aX;uJIF{e2G7 NH\MUYI1KGh? MU\JR|UxKD1iMT60PUDPxE1? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
SAE NGe3XGlHfW6ldHnvckBie3OjeR?= MY[0JIg> NYjROpdzUUN3MDC9JFEvPTFizszN NXHwTYlpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
U2OS NUDGcXptSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEXGR44yOiCq NIrjfWZKSzVyIE2gNU43OiEQvF2= NGjwcGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
SAE NEjIdm5HfW6ldHnvckBie3OjeR?= NGnIfGE1KGh? M4PxeWlEPTBiPTCxMlY{KM7:TR?= NIjMVFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
A549 MoPuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYWxNkBp NUXnW2RJUUN3MDC9JFEvPjdizszN MmLwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MCF7 NIrYWXJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkGwN{Bl[Xm| MYPHR|UxKD1iMT63JO69VQ>? NUD4WGFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
DU145 NFHjXoFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MXS5OkBp M4f4UmlEPTBiPTCyMlUzKM7:TR?= M1jydVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
DU145 M2\QZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVWxNkBp NXjVdHRQUUN3MDC9JFIvPTJizszN M{jaW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
T47D MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYSzJIRigXN? NWOydHhPT0N3MDC9JFIvQCEQvF2= MniwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
PC3 NYn3bnBWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYG1OVNSQTZiaB?= M1q2Z2lEPTBiPTCzMlAyKM7:TR?= NULjS|J{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
PC3 M2P3XmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYKxNkBp MmLETWM2OCB;IEOuNFEh|ryP NHLGOFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
HeLa NV;wRXNmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MoPSNVIhcA>? MVHJR|UxKD1iMz63OkDPxE1? M2rnb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
K562 MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWqzJIRigXN? MlLDS2M2OCB;IEOuPEDPxE1? NGLKN2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
A2780 NWPXN|A6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUSzJIRigXN? Mk\mS2M2OCB;IESg{txO M{fP[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
HL60 NIq2dodEgXSxdH;4bYNqfHliYYPzZZk> NVX1WZVDOjRiaB?= NWXGZW56UUN3MDC9JFUvOyEQvF2= NFHyfoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{K2Olk6QSd-MkeyOlY6QTl:L3G+
MV4-11 M1zVb2N6fG:2b4jpZ4l1gSCjc4PhfS=> MmLRNlQhcA>? NWntdFhMUUN3MDC9JFYvPCEQvF2= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ4Nkm5PUc,Ojd{Nk[5PVk9N2F-
K562 NXXXbpNySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MnHCO|IhcA>? MU\JR|UxKD1iOT6xNkDPxE1? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDNzNEWxN{c,Ojh|MUS1NVM9N2F-
OVCAR5 NWX4TFU4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF7vRog{KGSjeYO= M3\uPGdEPTBiPTCxNkDPxE1? M4P6clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID