(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2351.11 现货
RMB 2227.39 现货
RMB 4667.45 现货
RMB 11220.4 现货
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客户使用Selleck生产的(+)-JQ1发表文献273篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  NYroUYxFS2WubDDWbYFjcWyrdImgRZN{[Xl? MYmyOVAwPTByL{GwNFAhdk1? M3u3eVI1NzR6L{eyJIg> NInPfZJFVVOR Mk\WbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
BCPAP NILBVlhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYnhOXRSOjVyL{WwNE8yODByIH7N MlfPNlQwPDhxN{KgbC=> MUTEUXNQ NGXN[GpqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz NHX6WG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  NHG4cHVE\WyuIFP5Z4xmKEG|c3H5 NILzeXQzPTBxNUCwM|ExODBibl2= Mn;IO|IhcA>? MY\EUXNQ MYjhdpJme3S|IHPlcIwh[3mlbHWgZZQhTzBxR{GgdIhie2V? NGDGUIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
BCPAP NEHkZVdE\WyuIFP5Z4xmKEG|c3H5 NYjNdVExOjVyL{WwNE8yODByIH7N MlrOO|IhcA>? NW\ZXpdYTE2VTx?= M{fBXYFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
Hep3B MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITVT2oxNTFyIN88US=> M4HhW|Uh\A>? M1HQUmROW09? MkHRTWM2OD1yLkC4JO69VQ>? M4LFPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 Mn:2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLzNE0yOCEQvF2= NFnHWIY2KGR? NEm0VFhFVVOR Mmf4TWM2OD1yLkG0JO69VQ>? NUDVeYRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HuH7 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjXNE0yOCEQvF2= MUe1JIQ> M{DabmROW09? NGrqNI5KSzVyPUCuNlEh|ryP NGC5dVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HepG2 MoLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nyZVAuOTBizszN NF6xRZo2KGR? MULEUXNQ M1Kwc2lEPTB;MD6zOEDPxE1? NXTXVnZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
SMMC7721 NGnpbpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rBVlAuOTBizszN MmPHOUBl MlTWSG1UVw>? MV3JR|UxRTBwNEGg{txO MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
BEL7402 M2fPV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPSNE0yOCEQvF2= MnPLOUBl NFzLOmdFVVOR NIf3RXBKSzVyPUCuOFch|ryP MoO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
MHCC97H NFvZXYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLoNE0yOCEQvF2= MXu1JIQ> MUTEUXNQ MVjJR|UxRTBwNEGg{txO M3G4NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
Hep3B MlTuR4VtdCCFeXPs[UBCe3OjeR?= MVGwMlEwOC53L{KuOUDPxE1? NUn5eJhDPDhiaB?= MnfXSG1UVw>? M3Xs[Ixm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? NInHd2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 Mo\DR4VtdCCFeXPs[UBCe3OjeR?= NH31[o0xNjFxMD61M|IvPSEQvF2= NHTTXHc1QCCq MXnEUXNQ NV3tR3BVdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi NEC1ZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
Hep3B M4Tl[WFxd3C2b4Ppd{BCe3OjeR?= MWiwMlEwOC53L{KuOUDPxE1? M4XMcFQ5KGh? MYHEUXNQ M3rrZYFkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? MlrLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 NGnuVWxCeG:ydH;zbZMhSXO|YYm= MVywMlEwOC53L{KuOUDPxE1? NVXMZnk1PDhiaB?= MVjEUXNQ NFvoUpNi[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
A549 M{T3XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVKwMlEuOTBizszN MW[3NkBp NIfBTpNqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M13lSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknINE4yNTFyIN88US=> MlTOO|IhcA>? MUDpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NVvxeFFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 MnjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXLRlg{OC5zLUGwJO69VQ>? MYi3NkBp NFfMeGJqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? Ml;QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
A549 NG\xXWdHfW6ldHnvckBCe3OjeR?= NHPB[2IyNzJwNT:1JO69VQ>? NHrJRVUyOiCq NVXoTYxyyqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= MnTjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 MVrGeY5kfGmxbjDBd5NigQ>? NXHaXWpqOS9{LkWvOUDPxE1? NIK1NFUyOiCq NWPV[WNzyqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H157 M4fCW2Z2dmO2aX;uJGF{e2G7 NV3FeGFNOS9{LkWvOUDPxE1? MVuxNkBp NGfwWXRl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= M2HnZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MV;GeY5kfGmxbjDBd5NigQ>? MUSxM|IvPS93IN88US=> NV;lfmQxOTJiaB?= NF7TVWll\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= NEXE[m89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
C8161 NXS5O2dZS2WubDDWbYFjcWyrdImgRZN{[Xl? M{\H[FAuOiEQvF2= M1fIPVQh\A>? NEPsem5FVVOR Mlr6[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M2Kx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 M3zMSGNmdGxiVnnhZoltcXS7IFHzd4F6 M1raS|AuOiEQvF2= MXK0JIQ> NH[4VFdFVVOR M3zteYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Mel290 NX3KflN6S2WubDDWbYFjcWyrdImgRZN{[Xl? NV\MOlZFOC1{IN88US=> MWW0JIQ> M1i5eGROW09? MorV[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ Mn3oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 M4XvTGNmdGxiVnnhZoltcXS7IFHzd4F6 MUKwMVIh|ryP MV60JIQ> MnTnSG1UVw>? M{jnV4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MnPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 Mn3VR4VtdCCYaXHibYxqfHliQYPzZZk> MkLzNE0zKM7:TR?= MnnxOEBl NUfuZVRiTE2VTx?= NF3rSo1l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MmLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel202 M2i5fGNmdGxiVnnhZoltcXS7IFHzd4F6 NXHhbW8{OC1{IN88US=> NXrmUVl3PCCm Mk\lSG1UVw>? MWXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NED2cHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel270 NYfZe2VqS2WubDDWbYFjcWyrdImgRZN{[Xl? NFvmelUxNTJizszN NVfySm5VPCCm MXTEUXNQ NUjUXXVu\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M4rOT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1 MlXpR4VtdCCYaXHibYxqfHliQYPzZZk> MYSwMVIh|ryP NWfBW5E5PCCm MUTEUXNQ NGPkNZZl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NFzjV209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
92.1 MULBdI9xfG:|aYOgRZN{[Xl? MW[1NFAhdk1? M1WyWFQ5KGh? MnjFSG1UVw>? NVSydJFTcW6mdXPld{BieG:ydH;zbZM> NXPzUXVbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NXXiUYY6SXCxcITvd4l{KEG|c3H5 Mn3ROVAxKG6P NGDZVXk1QCCq M{XsWWROW09? MnL5bY5lfWOnczDhdI9xfG:|aYO= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 MULD[YxtKEO7Y3zlJGF{e2G7 Mm\ROVAxKG6P NE\4dGgzPC92OD:3NkBp NVnUb3loTE2VTx?= MmPPbY5lfWOnczD0bIUh[2WubDDhZ4N2dXWuYYTpc44h[XRic4XiMWcyyqB? MnHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
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MM1S NUT3VJI2S3m2b4TvfIlkcXS7IHHzd4F6 M{C5fFczKGh? NXnO[ZRrUUN3MDC9JFAvODJizszN M4XpelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
HT-29 MV\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NH;5T5gyOiCq MYDJR|UxKD1iMD6wNkDPxE1? NI\ufXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEWzOVA1PSd-Mki1N|UxPDV:L3G+
MV4-11 NFnpfmlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHGO|IhcA>? MnjVTWM2OCB;IECuNFI{KM7:TR?= M3rs[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW5OFI5Lz5{NUW1PVQzQDxxYU6=
MV4-11 Mon4R5l1d3SxeHnjbZR6KGG|c3H5 MlTDOEBl[Xm| MVvJR|UxKD1iMD6wNlQh|ryP M4TZTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
MV4-11 MlvkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU[0JIRigXN? M3vZd2lEPTBiPTCwMlAzPCEQvF2= NGDrVWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 NWnLSoR{TnWwY4Tpc44h[XO|YYm= NIXObmI{OCCvaX7z M4K2N2lEPTBiPTCwMlAzQDdizszN MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
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BL21(DE3)-R3-pRARE2 NX7ZPHR5TnWwY4Tpc44h[XO|YYm= M1XCVFMxKG2rboO= M37mVmlEPTBiPTCwMlA{OyEQvF2= M17iPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
BL21(DE3) M3HOOmZ2dmO2aX;uJIF{e2G7 NHzMTWlM\CB;IECuNFM1KM7:TR?= MlL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
Rosetta2 DE3 MmDLSox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? MlXVN|AhdWmwcx?= Mo[0TWM2OCB;IECuNFM2PyEQvF2= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 M3;pXGZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? Mne1N|AhdWmwcx?= MkTsTWM2OCB;IECuNFQzOiEQvF2= NIPwbXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
Rosetta2 DE3 MXPGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MnfGN|AhdWmwcx?= NULWZYlCUUN3MDC9JFAvODR4NzFOwG0> MlP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MOLM13 M3XRWWN6fG:2b4jpZ4l1gSCjc4PhfS=> MoTxOEBl[Xm| Ml3XTWM2OCB;IECuNFU3KM7:TR?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4M{S4O{c,Ojh2NkO0PFc9N2F-
MOLM13 MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWW2[ZdKPCCmYYnz M1jj[2lEPTBiPTCwMlA2PiEQvF2= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
HL60 NYXvfotVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEHPTJA4OiCq M{fVS2lEPTBiPTCwMlA3KM7:TR?= Mn;WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
HL60 NELSXpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWniToFnOyCmYYnz M2TmbmdEPTBiPTCwMlA3KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3N{C5PUc,Ojl4NUewPVk9N2F-
NALM6 NVXSUoo4S3m2b4TvfIlkcXS7IHHzd4F6 MVK1JIRigXN? MVXFR|UxKD1iMD6wOkDPxE1? Ml;lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
Raji MXjGeY5kfGmxbjDhd5NigQ>? M4m3XmlEPTBiPTCwMlA3KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Raji M2rPT2Z2dmO2aX;uJIF{e2G7 Ml[2OEBp NXPt[3RFUUN3MDC9JFAvODZ7IN88US=> NXvQdI5uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4PThpPkK0PVAxPzV6PD;hQi=>
MM1S NXvUW21RSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{nkTFczKGh? Mlm5TWM2OCB;IECuNFY6OSEQvF2= NXfmN2RWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlU1OzVpPkK5OVI2PDN3PD;hQi=>
BL21 (DE3)-codon plus-RIL NVLWdGdtTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MWfifUBndHWxcnXzZ4Vv[2ViYX7pd491em:yeTDhd5NigQ>? MnPrTWM2OCB;IECuNFch|ryP NGHGUXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlcyQCd-Mki1PFY4OTh:L3G+
22Rv1 NV3PWHhFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWXJXFl5QTZiaB?= MYXJR|UxKD1iMD6wO|Eh|ryP NUnwRpBtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
22Rv1 M{Dw[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MV2xNkBp M1zuU2lEPTBiPTCwMlA4OSEQvF2= MknhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
MV4-11 NGjQ[GJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MWLJR|UxKD1iMD6wO|Ih|ryP NY\iO5VxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3)-R3-pRARE2 MU\GeY5kfGmxbjDhd5NigQ>? MoLvN|AhdWmwcx?= MkfNTWM2OCB;IECuNFc4KM7:TR?= NVrmfZJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3)-R3-pRARE2 MV7GeY5kfGmxbjDhd5NigQ>? NVPISm02OzBibXnudy=> NYnW[W9wUUN3MDC9JFAvODd5IN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF7NUeyN{c,OjhzOUW3NlM9N2F-
BL21(DE3)-R3-pRARE2 M1HsW2Z2dmO2aX;uJIF{e2G7 MYOzNEBucW6| M2PrT2lEPTBiPTCwMlA4PyEQvF2= NXyxdVRwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3O|Y5OzRpPkK5O|c3QDN2PD;hQi=>
MV4-11 MWDDfZRwfG:6aXPpeJkh[XO|YYm= M1zyd|I1KGh? M1zqZWdKPTBiPTCwMlA5KM7:TR?= NI\oepI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG5NVM3Oyd-Mk[xPVE{PjN:L3G+
MV411 MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MoHxO|IhcA>? MlzwTWM2OCB;IECuNFgh|ryP M1TLWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{G0OVE{Lz5{OEOxOFUyOzxxYU6=
TY82 NVLTcHFHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NELuXog4OiCq NGjr[mZKSzVyIE2gNE4xQDB6IN88US=> M13NN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK1OFM2Lz5{OUWyOVQ{PTxxYU6=
HL60 Moi3SpVv[3Srb36gZZN{[Xl? NE\BZYIzPCCq NXP2b4JXUUN3MDC9JFAvODh4IN88US=> MmXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
697 NYPWfFhrS3m2b4TvfIlkcXS7IHHzd4F6 NILIPIs2KGSjeYO= NF7lUFJGSzVyIE2gNE4xQSEQvF2= NWfxSYZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
Loucy NXvvNVVuS3m2b4TvfIlkcXS7IHHzd4F6 MVG1JIRigXN? MV7FR|UxKD1iMD6wPUDPxE1? M3LITFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3) MXPGeY5kfGmxbjDhd5NigQ>? NUPoSJVOU2RiPTCwMlA6OiEQvF2= NXHnbYRiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
BL21(DE3)-R3-pRARE2 NUTPdmZkTnWwY4Tpc44h[XO|YYm= MYjL[EA:KDBwMTFOwG0> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV7NUCwO{c,Ojh3OUWwNFc9N2F-
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MM1S M4nZVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml3VO|IhcA>? M4PkZmlEPTBiPTCwMlExQSEQvF2= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
LNCAP cells MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2HrXGlEPTBiPTCwMlExQTZizszN MnmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
T cells MnuxSpVv[3Srb36gZZN{[Xl? MVGyOEBp NILUeHZKSzVyIE2gNE4yOSEQvF2= M4Psd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{G0OVE{Lz5{OEOxOFUyOzxxYU6=
HL60 M2DCUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M4W5d|czKGh? M1XQUGlEPTBiPTCwMlEyKM7:TR?= NH:ydpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki2PVE6PCd-Mk[4OlkyQTR:L3G+
BL21(DE3) MUjGeY5kfGmxbjDhd5NigQ>? NEfJZY5KSzVyIE2gNE4yOiEQvF2= Mn;iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
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H1299 MV3GeY5kfGmxbjDhd5NigQ>? MkDMNlQhcA>? MWnFR|UxKD1iMD6xOVMh|ryP MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEm1NlEoRjJ6OUS5OVIyRC:jPh?=
HD-MB03 NF7RXYxEgXSxdH;4bYNqfHliYYPzZZk> MX21JIRigXN? NHnRN|FGSzVyIE2gNE4yPiEQvF2= NX7abIVLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP M2XYR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NV;IZpJRQTZiaB?= NHXPbVFKSzVyIE2gNE4yPiEQvF2= MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
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MV4-11 M4r0eWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFu0WHUyOiCq Mmn5TWM2OCB;IECuNVYh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
LNCAP M{XLNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2Xq[VEzKGh? M36yPGlEPTBiPTCwMlE3KM7:TR?= M131ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
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C4-2B Mkm1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NV\JOJFsOTJiaB?= NYDzWHNQUUN3MDC9JFAvOTlizszN NWHyTIhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
Vero E6 NXf4OXd4SW62aY\pdoFtKGGldHn2bZR6KGG|c3H5 NGLFc|c1QCCq M37tcGlEPTBiPTCwMlE6Ojd3IN88US=> MmfVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzJ|NUO4OVkoRjN{M{WzPFU6RC:jPh?=
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MX1 M{HLWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWm3NkBp Ml7pSWM2OCB;IECuNlU1KM7:TR?= MmXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEm1NlEoRjJ6OUS5OVIyRC:jPh?=
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MDA-MB-231 NXW4WYVqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV3jOmlDOyCmYYnz Mof3S2M2OCB;IECuN{DPxE1? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
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SAE NWLTb|hETnWwY4Tpc44h[XO|YYm= MkiyOEBp NYTlXmtzUUN3MDC9JFEvOzhizszN MlrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NEm3OFEoRjJ7NkS5O|QyRC:jPh?=
SAE NGLTWZVHfW6ldHnvckBie3OjeR?= MljEOEBp M{PJcWlEPTBiPTCxMlQ6KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
SAE M3HMUGZ2dmO2aX;uJIF{e2G7 MUS0JIg> NF;VNVZKSzVyIE2gNU42OSEQvF2= NFL3Zpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0PVc1OSd-Mkm2OFk4PDF:L3G+
U2OS NF;3fYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NEnjfZEyOiCq NFrI[XlKSzVyIE2gNU43OiEQvF2= NIrIdJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
SAE MVXGeY5kfGmxbjDhd5NigQ>? Mm\UOEBp MV;JR|UxKD1iMT62N{DPxE1? MmftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NEm3OFEoRjJ7NkS5O|QyRC:jPh?=
A549 NWL3VJNKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mo[wNVIhcA>? MnXFTWM2OCB;IEGuOlch|ryP NH3GcVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
MCF7 MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnvoN{Bl[Xm| NHrrNYNISzVyIE2gNU44KM7:TR?= M{HiZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
DU145 Ml\5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NIrWWpI6PiCq NGfNUldKSzVyIE2gNk42OiEQvF2= MoqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
DU145 NWj1b3NPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NFnqV3UyOiCq MmHBTWM2OCB;IEKuOVIh|ryP NVrYbVBsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
T47D MkjzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlLyN{Bl[Xm| M33nUWdEPTBiPTCyMlgh|ryP MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
PC3 MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MV25OkBp M4LNcmlEPTBiPTCzMlAyKM7:TR?= NGm4XnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
PC3 MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWDnfVY{OTJiaB?= MVXJR|UxKD1iMz6wNUDPxE1? M{LQcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
HeLa NYG2W5F{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEPnfZkyOiCq M{D0N2lEPTBiPTCzMlc3KM7:TR?= M{fLWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
K562 NIrqVodIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1\MVlMh\GG7cx?= NVi5[5FkT0N3MDC9JFMvQCEQvF2= NWTiUolbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
A2780 MmPHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mnm5N{Bl[Xm| NFLiTm9ISzVyIE2gOEDPxE1? MkHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
HL60 MlvOR5l1d3SxeHnjbZR6KGG|c3H5 MmLlNlQhcA>? NIjJb5JKSzVyIE2gOU4{KM7:TR?= Ml;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Nk[5PVkoRjJ5Mk[2PVk6RC:jPh?=
MV4-11 MXHDfZRwfG:6aXPpeJkh[XO|YYm= NEHDbmgzPCCq NH\pXXFKSzVyIE2gOk41KM7:TR?= MorkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Nk[5PVkoRjJ5Mk[2PVk6RC:jPh?=
K562 M32zPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlrxO|IhcA>? MoDhTWM2OCB;IEmuNVIh|ryP M{e0ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{G0OVE{Lz5{OEOxOFUyOzxxYU6=
OVCAR5 NVvMW|UyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrENW9wOyCmYYnz Ml\IS2M2OCB;IEGyJO69VQ>? Moe3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

Tags: 购买(+)-JQ1 | (+)-JQ1供应商 | 采购(+)-JQ1 | (+)-JQ1价格 | (+)-JQ1生产 | 订购(+)-JQ1 | (+)-JQ1代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID