(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2351.11 现货
RMB 2227.39 现货
RMB 4667.45 现货
RMB 11220.4 现货
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客户使用Selleck生产的(+)-JQ1发表文献297篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MVnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MlzNNlUxNzVyMD:xNFAxKG6P NXm1W4NCOjRxNEivO|IhcA>? MYnEUXNQ NV71[mtwcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgbY4h[m:2aDDkc5NmNSCjbnSgeIlu\S1iZHXw[Y5l\W62IH3hco5meg>? M{fHRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP Mnv4R4VtdCCYaXHibYxqfHliQYPzZZk> MnPCNlUxNzVyMD:xNFAxKG6P MmTLNlQwPDhxN{KgbC=> M3rEN2ROW09? MoPBbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> NHzNNY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  NGTBS|hE\WyuIFP5Z4xmKEG|c3H5 NIX5TVIzPTBxNUCwM|ExODBibl2= NYnFSY92PzJiaB?= MlHUSG1UVw>? MkX4ZZJz\XO2czDj[YxtKGO7Y3zlJIF1KEdyL1exJJBp[XOn NX7oPHBIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
BCPAP NUPKcWd6S2WubDDDfYNt\SCDc4PhfS=> NFvpcJYzPTBxNUCwM|ExODBibl2= M1W1WVczKGh? NI[4WoVFVVOR MnTRZZJz\XO2czDj[YxtKGO7Y3zlJIF1KEdyL1exJJBp[XOn NXHKSld6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
Hep3B MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojHNE0yOCEQvF2= NUe4[ZJ{PSCm MYXEUXNQ NYn0dIJIUUN3ME2wMlA5KM7:TR?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 MmHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWKwMVExKM7:TR?= M1rYd|Uh\A>? NYTSTW5[TE2VTx?= NIXZfoVKSzVyPUCuNVQh|ryP NYDoRmxTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HuH7 NWPUV|dZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTsT2IxNTFyIN88US=> NVn6RYJjPSCm MonqSG1UVw>? NUPZcYU5UUN3ME2wMlIyKM7:TR?= NG\LNVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HepG2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7KcpIxNTFyIN88US=> NGD2bpI2KGR? M{TVPGROW09? NV;1blhKUUN3ME2wMlM1KM7:TR?= Ml60QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
SMMC7721 M4DFe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVWwMVExKM7:TR?= MY[1JIQ> NX2wO2lSTE2VTx?= M1XufmlEPTB;MD60NUDPxE1? NFvRRoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
BEL7402 NXi0[3ZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17Cc|AuOTBizszN NWLMV2dbPSCm MV\EUXNQ Ml3WTWM2OD1yLkS3JO69VQ>? M13MVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
MHCC97H MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnZSWMxNTFyIN88US=> MYC1JIQ> MYrEUXNQ MXLJR|UxRTBwNEGg{txO NYTjNYl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B M4\IbWNmdGxiQ4njcIUhSXO|YYm= M1f3XlAvOS9yLkWvNk42KM7:TR?= NHXkSIw1QCCq Ml7QSG1UVw>? MXLs[YFleyC2bzDhJJN2[nO2YX70bYFtKGGlY4XteYxifGmxbjDv[kBJS0NiY3XscJMhcW5ic4XiMWcyKHCqYYPlxsA> NEXkRYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 MYnD[YxtKEO7Y3zlJGF{e2G7 NXzlTGhDOC5zL{CuOU8zNjVizszN Mo[5OFghcA>? NFzqO2xFVVOR NIDLcGpt\WGmczD0c{BiKHO3YoP0ZY51cWGuIHHjZ5VufWyjdHnvckBw\iCKQ1OgZ4VtdHNiaX6gd5VjNUdzIIDoZZNmyqB? NV6zRmlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B M1;LSWFxd3C2b4Ppd{BCe3OjeR?= NVfUb5NyOC5zL{CuOU8zNjVizszN NUPi[5U5PDhiaB?= MnzxSG1UVw>? NWnkO2VY[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= MonLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 MmOyRZBweHSxc3nzJGF{e2G7 MXmwMlEwOC53L{KuOUDPxE1? MlzGOFghcA>? NYPiXVV1TE2VTx?= M3jre4FkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? NU\ZR2sxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
A549 MlnzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljNNE4yNTFyIN88US=> NWPiTlFkPzJiaB?= M3;qRolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz Ml;nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 NETiUI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkP5NE4yNTFyIN88US=> MnPPO|IhcA>? M1HxOIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NWn5WXlIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XEZlAvOS1zMDFOwG0> NVr5T4FmPzJiaB?= NGqwcZNqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M3zPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
A549 MXHGeY5kfGmxbjDBd5NigQ>? MmH0NU8zNjVxNTFOwG0> NVXCeGUyOTJiaB?= NHzDbIjDqHenYXvsfUBl\WO{ZXHz[YQhSmOuLUKgcIV3\Wy| NFXNTIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
H1299 MVfGeY5kfGmxbjDBd5NigQ>? M3ntNVEwOi53L{Wg{txO NWXjXWl1OTJiaB?= MoDqxsB4\WGtbImg[IVkemWjc3XkJGJkdC1{IHzleoVtew>? NXPGTnFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H157 MVfGeY5kfGmxbjDBd5NigQ>? MkP3NU8zNjVxNTFOwG0> NVzKRVlEOTJiaB?= M3PCeoRm[3KnYYPl[EBFWjRiZYjwdoV{e2mxbh?= NXT3VnR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 NFrVT|VHfW6ldHnvckBCe3OjeR?= MlfnNU8zNjVxNTFOwG0> NFzZUpgyOiCq MkD0[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w NVf6TZRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
C8161 NHv4PWhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYGwMVIh|ryP Mk\uOEBl M2HLO2ROW09? MUjk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M2DidVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel285 NITQXo5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? MXywMVIh|ryP NE\GU3U1KGR? NGjaNppFVVOR M4DGU4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= Mn\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel290 MV7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUnmS4tCOC1{IN88US=> NX3m[mN[PCCm MornSG1UVw>? NF;0VpRl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NVTlNlJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
92.1 MUfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NInpV4oxNTJizszN NHvL[pI1KGR? NUf0fXl3TE2VTx?= NWrXOYl4\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NHzmb4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1.3 NG[3OYRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUmwMVIh|ryP NFn2XHA1KGR? MknoSG1UVw>? NHHoeVZl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M{\xd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel202 NXXtSHJZS2WubDDWbYFjcWyrdImgRZN{[Xl? MnXMNE0zKM7:TR?= NVvMdHZrPCCm MULEUXNQ NFrsbGxl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NIXZU2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel270 M{f0UWNmdGxiVnnhZoltcXS7IFHzd4F6 NFj6[XkxNTJizszN NF7rWJo1KGR? NHvC[mFFVVOR NUmwPI54\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
Omm1 NGXJU5NE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX[wMVIh|ryP M3zCclQh\A>? NEPvUWZFVVOR NIPwWJhl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
92.1 MoTiRZBweHSxc3nzJGF{e2G7 NVvEOnhMPTByIH7N NGDO[lg1QCCq NE\JbpBFVVOR M3XPS4lv\HWlZYOgZZBweHSxc3nz NGfP[3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Omm1.3 MU\BdI9xfG:|aYOgRZN{[Xl? NGnTflI2ODBibl2= NWXo[o16PDhiaB?= NUXBbGZETE2VTx?= NWm4bVRDcW6mdXPld{BieG:ydH;zbZM> NEHjOFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
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MM1S NES0dGpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYHSUYN4PzJiaB?= NUiyc2pHUUN3MDC9JFAvODF7IN88US=> M4njSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUi2O|E5Lz5{OEW4OlcyQDxxYU6=
MM1S M2jDTGN6fG:2b4jpZ4l1gSCjc4PhfS=> MUi3NkBp M2C3bmlEPTBiPTCwMlAzKM7:TR?= NYDJV4w5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
HT-29 Mm\ERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVyxNkBp Mne2TWM2OCB;IECuNFIh|ryP NF7KVFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEWzOVA1PSd-Mki1N|UxPDV:L3G+
MV4-11 NXLlWpBsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUm3NkBp M1juUmlEPTBiPTCwMlAzOyEQvF2= NVL2XnlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MV4-11 NVXYS4k2S3m2b4TvfIlkcXS7IHHzd4F6 M{frcVQh\GG7cx?= MnnrTWM2OCB;IECuNFI1KM7:TR?= NWLZZY9kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MV4-11 MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2H5flQh\GG7cx?= MWHJR|UxKD1iMD6wNlQh|ryP MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 MkfkSpVv[3Srb36gZZN{[Xl? M1nzelMxKG2rboO= Mo\mTWM2OCB;IECuNFI5PyEQvF2= NXn5UIdpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
NALM16 M4nrW2N6fG:2b4jpZ4l1gSCjc4PhfS=> NUXYb5Y2PSCmYYnz MlTRSWM2OCB;IECuNFMh|ryP M3m1cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3)-R3-pRARE2 NUnzXYVnTnWwY4Tpc44h[XO|YYm= NHHVSW4{OCCvaX7z M3\QcmlEPTBiPTCwMlA{OyEQvF2= M2\6R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
BL21(DE3) MnXRSpVv[3Srb36gZZN{[Xl? M3XHWGtlKD1iMD6wN|Qh|ryP NWr5cW5iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
Rosetta2 DE3 NX\hVlhWTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NX\MNXZSOzBibXnudy=> MmGwTWM2OCB;IECuNFM2PyEQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 MmT3Sox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? M1XyW|MxKG2rboO= NHi2Z41KSzVyIE2gNE4xPDJ{IN88US=> NVHIV|NMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
Rosetta2 DE3 NYLqTpJCTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= MYGzNEBucW6| Mn3DTWM2OCB;IECuNFQ3PyEQvF2= NVrDUFdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MOLM13 NHLoXJJEgXSxdH;4bYNqfHliYYPzZZk> NXnuVppHPCCmYYnz MnfYTWM2OCB;IECuNFU3KM7:TR?= Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MOLM13 MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlfNOEBl[Xm| NVP3WmxNUUN3MDC9JFAvODV4IN88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
HL60 M3[3dmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFu0cXU4OiCq NU\SdJM4UUN3MDC9JFAvODZizszN MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
HL60 MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV3ubVA4OyCmYYnz M4D1VmdEPTBiPTCwMlA3KM7:TR?= M2nR[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkW3NFk6Lz5{OU[1O|A6QTxxYU6=
NALM6 NYrHZY9NS3m2b4TvfIlkcXS7IHHzd4F6 NInRZXc2KGSjeYO= MnXxSWM2OCB;IECuNFYh|ryP M1K2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
Raji NVfsd5hUTnWwY4Tpc44h[XO|YYm= MmDPTWM2OCB;IECuNFYh|ryP MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Raji MoHJSpVv[3Srb36gZZN{[Xl? Mkf5OEBp NWHWZ|Y{UUN3MDC9JFAvODZ7IN88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEe1PEc,OjR7MEC3OVg9N2F-
MM1S MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MUS3NkBp M3yyVGlEPTBiPTCwMlA3QTFizszN MkPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
BL21 (DE3)-codon plus-RIL NVPuS28yTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= M3jhS4J6KG[udX;y[ZNk\W6lZTDhcol{d3S{b4D5JIF{e2G7 MWXJR|UxKD1iMD6wO{DPxE1? NIPmVYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlcyQCd-Mki1PFY4OTh:L3G+
22Rv1 MV\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M{XaRlk3KGh? NW\lUnE5UUN3MDC9JFAvODdzIN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
22Rv1 NITzXJVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4CxbFEzKGh? MYnJR|UxKD1iMD6wO|Eh|ryP NX\SWW5QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
MV4-11 NIj6UFJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYPzT2V4UUN3MDC9JFAvODd{IN88US=> MlLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzOUW3NlMoRjJ6MUm1O|I{RC:jPh?=
BL21(DE3)-R3-pRARE2 NIq4cJJHfW6ldHnvckBie3OjeR?= MnHaN|AhdWmwcx?= NX;JTo9RUUN3MDC9JFAvODd5IN88US=> NH7kUok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkezNVQ6OCd-Mk[3N|E1QTB:L3G+
BL21(DE3)-R3-pRARE2 NHTpR|BHfW6ldHnvckBie3OjeR?= NFLXe48{OCCvaX7z M360VGlEPTBiPTCwMlA4PyEQvF2= NX7YW25iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ|PD;hQi=>
BL21(DE3)-R3-pRARE2 MYXGeY5kfGmxbjDhd5NigQ>? NIrlOJQ{OCCvaX7z M4rZ[WlEPTBiPTCwMlA4PyEQvF2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd5NkizOEc,Ojl5N{[4N|Q9N2F-
MV4-11 M1\heWN6fG:2b4jpZ4l1gSCjc4PhfS=> NUfDfYFMOjRiaB?= MV;HTVUxKD1iMD6wPEDPxE1? NVfzb5M2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVE{PjNpPkK2NVkyOzZ|PD;hQi=>
MV411 NEL6OnlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MX:3NkBp NEPU[ldKSzVyIE2gNE4xQCEQvF2= NF34b2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
TY82 NFjWfXZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NIPNdWE4OiCq NE\mfmhKSzVyIE2gNE4xQDB6IN88US=> MnPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MkW0N|UoRjJ7NUK1OFM2RC:jPh?=
HL60 NV;jSZRETnWwY4Tpc44h[XO|YYm= M4jlZlI1KGh? MUjJR|UxKD1iMD6wPFYh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
697 M2T6SmN6fG:2b4jpZ4l1gSCjc4PhfS=> MVO1JIRigXN? MYPFR|UxKD1iMD6wPUDPxE1? NFr1VlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
Loucy MmHwR5l1d3SxeHnjbZR6KGG|c3H5 NF\uboY2KGSjeYO= MlnNSWM2OCB;IECuNFkh|ryP NGjXenU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
BL21(DE3) NVr3Xm5GTnWwY4Tpc44h[XO|YYm= Ml:wT4QhRSByLkC5NkDPxE1? Mo\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
BL21(DE3)-R3-pRARE2 MYPGeY5kfGmxbjDhd5NigQ>? M1\BSGtlKD1iMD6xJO69VQ>? NGH2WGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW5OVAxPyd-Mki1PVUxODd:L3G+
HT-29 MnLTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV3V[ppZPzJiaB?= MUPJR|UxKD1iMD6xNFQh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
MM1S NFXvWZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MY[3NkBp NG\VSVJKSzVyIE2gNE4yODlizszN NWP6bG5tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
LNCAP cells M4rLdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnPLTWM2OCB;IECuNVA6PiEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
T cells M1;pcGZ2dmO2aX;uJIF{e2G7 NXHSZVZHOjRiaB?= NHrzOJRKSzVyIE2gNE4yOSEQvF2= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDNzNEWxN{c,Ojh|MUS1NVM9N2F-
HL60 MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NYfHflNmPzJiaB?= NFqxRopKSzVyIE2gNE4yOSEQvF2= NHznNXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki2PVE6PCd-Mk[4OlkyQTR:L3G+
BL21(DE3) Ml;1SpVv[3Srb36gZZN{[Xl? NXu0elZ6UUN3MDC9JFAvOTJizszN NYDHR4huRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3) MnnqSpVv[3Srb36gZZN{[Xl? M2DENVIvPSCq NIHzN5NKSzVyIE2gNE4yOiEQvF2= M4mwdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
H1299 MYrGeY5kfGmxbjDhd5NigQ>? MUCyOEBp MVjFR|UxKD1iMD6xOVMh|ryP MmS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEm1NlEoRjJ6OUS5OVIyRC:jPh?=
HD-MB03 MoHjR5l1d3SxeHnjbZR6KGG|c3H5 M2jkW|Uh\GG7cx?= NETUbFVGSzVyIE2gNE4yPiEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
LNCAP M2HnPGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 Mli1PVYhcA>? NUDwNmtzUUN3MDC9JFAvOTZizszN M3rD[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
Hs578T NULXbJU6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NInXfXMyOiCq M4L4U2lEPTBiPTCwMlE3KM7:TR?= MoLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MV4-11 MlXDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MUGxNkBp NIPXPGhKSzVyIE2gNE4yPiEQvF2= M4jkdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
LNCAP M3;LWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M{niSVEzKGh? M3vYb2lEPTBiPTCwMlE3KM7:TR?= M1PRfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
C4-2B M{ewVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkTPPVYhcA>? Mly1TWM2OCB;IECuNVkh|ryP M4nEclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
C4-2B NGDkNphCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlL0NVIhcA>? NIPUSVlKSzVyIE2gNE4yQSEQvF2= NVSzfWJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
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OVCAR5 M17VTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYn2RpU5OyCmYYnz NIf2SY1ISzVyIE2gNVIh|ryP NXXSSldmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

Tags: 购买(+)-JQ1 | (+)-JQ1供应商 | 采购(+)-JQ1 | (+)-JQ1价格 | (+)-JQ1生产 | 订购(+)-JQ1 | (+)-JQ1代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID