(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2351.11 现货
RMB 2227.39 现货
RMB 4667.45 现货
RMB 11220.4 现货
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客户使用Selleck生产的(+)-JQ1发表文献317篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MmO4R4VtdCCYaXHibYxqfHliQYPzZZk> M3LRRVI2OC93MECvNVAxOCCwTR?= NGfNVm0zPC92OD:3NkBp NF;Nc4NFVVOR M1L2bolvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> M{LsblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP M1naVWNmdGxiVnnhZoltcXS7IFHzd4F6 NWXIcY9XOjVyL{WwNE8yODByIH7N MUeyOE81QC95MjDo NVTIXoI1TE2VTx?= M3n4PYlvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> NGrPUIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  NG[xUmhE\WyuIFP5Z4xmKEG|c3H5 M1XGe|I2OC93MECvNVAxOCCwTR?= MknGO|IhcA>? Ml;iSG1UVw>? MULhdpJme3S|IHPlcIwh[3mlbHWgZZQhTzBxR{GgdIhie2V? M1\2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP M2K4ZmNmdGxiQ4njcIUhSXO|YYm= MYKyOVAwPTByL{GwNFAhdk1? MXe3NkBp M2HJdGROW09? M4PZWIFzemW|dIOgZ4VtdCCleXPs[UBifCCJMD;HNUBxcGG|ZR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyN{i4NUc,OjZ5MEe4PFE9N2F-
Hep3B NWPCc5dxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;4NE0yOCEQvF2= Mo\5OUBl MVHEUXNQ NXv2[3FUUUN3ME2wMlA5KM7:TR?= M{jmfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HCCLM3 M13MNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETQNFIxNTFyIN88US=> NHfDbGE2KGR? MliwSG1UVw>? NVK2VGp4UUN3ME2wMlE1KM7:TR?= MoDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HuH7 NFHuPYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDEbYkxNTFyIN88US=> MUS1JIQ> M{S4VGROW09? NUfwc4dOUUN3ME2wMlIyKM7:TR?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HepG2 Ml\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvSXZI4OC1zMDFOwG0> MYC1JIQ> NX64[pZFTE2VTx?= M4HJRWlEPTB;MD6zOEDPxE1? MnnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
SMMC7721 MnXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVOwMVExKM7:TR?= NE\mT242KGR? MUHEUXNQ MWfJR|UxRTBwNEGg{txO NUXXeY1xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
BEL7402 NXHpRY9GT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4Hv[VAuOTBizszN M{GydVUh\A>? NI\5SIxFVVOR M2i3[WlEPTB;MD60O{DPxE1? NXvUN5dCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
MHCC97H NWf0[G9wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVewMVExKM7:TR?= MVu1JIQ> MYXEUXNQ NUW2OJZLUUN3ME2wMlQyKM7:TR?= NV;MdHlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B MW\D[YxtKEO7Y3zlJGF{e2G7 M3XGelAvOS9yLkWvNk42KM7:TR?= Mn3SOFghcA>? MoLmSG1UVw>? M{G0dIxm[WS|IITvJIEhe3Wkc4ThcpRq[WxiYXPjeY12dGG2aX;uJI9nKEiFQzDj[YxteyCrbjDzeYIuTzFicHjhd4XDqA>? Mlj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
HCCLM3 NXLwVVJUS2WubDDDfYNt\SCDc4PhfS=> NWLqZok1OC5zL{CuOU8zNjVizszN MXK0PEBp NHqyT2tFVVOR MV3s[YFleyC2bzDhJJN2[nO2YX70bYFtKGGlY4XteYxifGmxbjDv[kBJS0NiY3XscJMhcW5ic4XiMWcyKHCqYYPlxsA> NXXSNIVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
Hep3B MXzBdI9xfG:|aYOgRZN{[Xl? NIjEW4MxNjFxMD61M|IvPSEQvF2= NW\kW5JpPDhiaB?= NH7KeIlFVVOR NULTUFVE[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NELMT3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 M3\ZV2Fxd3C2b4Ppd{BCe3OjeR?= NIf4WZgxNjFxMD61M|IvPSEQvF2= NVLYblR7PDhiaB?= M4rxT2ROW09? NX[xflBU[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NGfBRWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
A549 M{nTcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVGwMlEuOTBizszN M1\PWFczKGh? M2KzVolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M3zhTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H157 M4rwR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\vNE4yNTFyIN88US=> MlP2O|IhcA>? NUPWVY57cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3rPd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 MofJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fWZVAvOS1zMDFOwG0> NHzTcmw4OiCq M2HaSIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MnGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
A549 MWLGeY5kfGmxbjDBd5NigQ>? NHfJSZkyNzJwNT:1JO69VQ>? Ml3PNVIhcA>? NHv5VZPDqHenYXvsfUBl\WO{ZXHz[YQhSmOuLUKgcIV3\Wy| Mmn0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 NF23ZpVHfW6ldHnvckBCe3OjeR?= MYOxM|IvPS93IN88US=> NVLIOY9ZOTJiaB?= MUFCpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H157 NX\DdnpGTnWwY4Tpc44hSXO|YYm= NUHSdo9qOS9{LkWvOUDPxE1? NHfYT5YyOiCq NH;nc|dl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= NWHlTGxGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NVUzOjVpPkK2OFE2OjJ3PD;hQi=>
H1299 Mnv2SpVv[3Srb36gRZN{[Xl? NYTzeYxpOS9{LkWvOUDPxE1? M4rBVFEzKGh? M4XvUIRm[3KnYYPl[EBFWjRiZYjwdoV{e2mxbh?= NEDJ[Yg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkSxOVIzPSd-Mk[0NVUzOjV:L3G+
C8161 MYnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{Lv[FAuOiEQvF2= NWTyTFNKPCCm NH7jepJFVVOR Mofx[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MnzPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel285 NFv3eFFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWi4OZBUOC1{IN88US=> NFHZWpE1KGR? MVPEUXNQ MkfE[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NYjnd25PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel290 M2DVcmNmdGxiVnnhZoltcXS7IFHzd4F6 NYnPPZNLOC1{IN88US=> Mn\vOEBl NFvSNYtFVVOR NX36VoNI\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2TJPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
92.1 NXXHNWp1S2WubDDWbYFjcWyrdImgRZN{[Xl? NEnocYYxNTJizszN NVXSZY9tPCCm MVzEUXNQ NGTD[Gll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3LDR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1.3 MYHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M33YNVAuOiEQvF2= M3vEb|Qh\A>? MnfkSG1UVw>? NIfoPXZl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NVrFWFJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Mel202 MkDYR4VtdCCYaXHibYxqfHliQYPzZZk> NYXRVnpIOC1{IN88US=> MYO0JIQ> NFP0dWJFVVOR MWTk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M2ftSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Mel270 Mn\yR4VtdCCYaXHibYxqfHliQYPzZZk> M2CyU|AuOiEQvF2= Mn24OEBl M2jsdmROW09? M3;rRYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NVfmVHZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1 NHXlTHFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2j4dVAuOiEQvF2= MnTpOEBl NF7mRnBFVVOR NXi0U4JJ\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MlvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 NITuR4dCeG:ydH;zbZMhSXO|YYm= MmfJOVAxKG6P NV7mdllIPDhiaB?= MYfEUXNQ NFTNR49qdmS3Y3XzJIFxd3C2b4Ppdy=> Ml[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Omm1.3 MlrFRZBweHSxc3nzJGF{e2G7 NXOzTWRiPTByIH7N NEDwdY41QCCq NV21XYxrTE2VTx?= NIT6NJZqdmS3Y3XzJIFxd3C2b4Ppdy=> NXXTN3l4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
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MM1S M4OxWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnPJO|IhcA>? NF2wd5lKSzVyIE2gNE4xOTlizszN MlvrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OE[3NVgoRjJ6NUi2O|E5RC:jPh?=
MM1S MXnDfZRwfG:6aXPpeJkh[XO|YYm= NVmyN3ptPzJiaB?= MnLmTWM2OCB;IECuNFIh|ryP MnfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
HT-29 M{C4R2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NXPLdGVCOTJiaB?= MVjJR|UxKD1iMD6wNkDPxE1? MnG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3M{WwOFUoRjJ6NUO1NFQ2RC:jPh?=
MV4-11 M2jBe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWi3NkBp MXzJR|UxKD1iMD6wNlMh|ryP MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
MV4-11 MmLCR5l1d3SxeHnjbZR6KGG|c3H5 MkHoOEBl[Xm| M2\0SWlEPTBiPTCwMlAzPCEQvF2= NI[5PJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MV4-11 M1jLXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWK0JIRigXN? NGfZbJVKSzVyIE2gNE4xOjRizszN NYfFOWxqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 M3SwXWZ2dmO2aX;uJIF{e2G7 NHnlfpU{OCCvaX7z MULJR|UxKD1iMD6wNlg4KM7:TR?= NYDDVIVvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
NALM16 NY\zc4VCS3m2b4TvfIlkcXS7IHHzd4F6 NWXOTm5UPSCmYYnz Ml3KSWM2OCB;IECuNFMh|ryP NGDKO4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
BL21(DE3)-R3-pRARE2 NWq2boN1TnWwY4Tpc44h[XO|YYm= NV;2PWpXOzBibXnudy=> MkfQTWM2OCB;IECuNFM{KM7:TR?= MoOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzOUW3NlMoRjJ6MUm1O|I{RC:jPh?=
BL21(DE3) NIXlNWRHfW6ldHnvckBie3OjeR?= NXPYWYk5U2RiPTCwMlA{PCEQvF2= NX3ReYJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
Rosetta2 DE3 NYOxZWdWTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NV3rNFBXOzBibXnudy=> M3\KemlEPTBiPTCwMlA{PTdizszN NFrTNpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 M3vaRWZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? MVyzNEBucW6| NVq0ZYw6UUN3MDC9JFAvODR{MjFOwG0> M{P5OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NE[zOFg4Lz5{OES2N|Q5PzxxYU6=
Rosetta2 DE3 MWXGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 NF[xbHE{OCCvaX7z M3rBemlEPTBiPTCwMlA1PjdizszN NWfSNZBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MOLM13 MmDFR5l1d3SxeHnjbZR6KGG|c3H5 M2XMSlQh\GG7cx?= NUHOSXoxUUN3MDC9JFAvODV4IN88US=> NV7E[2d5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MOLM13 NUDJe5pFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEPpSHY1KGSjeYO= M3;I[mlEPTBiPTCwMlA2PiEQvF2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
HL60 MnyzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlfXO|IhcA>? MoDBTWM2OCB;IECuNFYh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MECyOEc,OjlzN{CwNlQ9N2F-
HL60 NWXUepNQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MorJN{Bl[Xm| MXHHR|UxKD1iMD6wOkDPxE1? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3N{C5PUc,Ojl4NUewPVk9N2F-
NALM6 Mnr5R5l1d3SxeHnjbZR6KGG|c3H5 MV61JIRigXN? NFTybFZGSzVyIE2gNE4xPiEQvF2= M3fMXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
Raji MWPGeY5kfGmxbjDhd5NigQ>? NV3uV|NQUUN3MDC9JFAvODZizszN M17CR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
Raji NYq1R|dVTnWwY4Tpc44h[XO|YYm= M2fSVVQhcA>? MmXyTWM2OCB;IECuNFY6KM7:TR?= M3rlRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO|U5Lz5{NEmwNFc2QDxxYU6=
MM1S NGTJRZNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M{ewNVczKGh? MVPJR|UxKD1iMD6wOlkyKM7:TR?= NH:2eZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOVQ{PSd-Mkm1NlU1OzV:L3G+
BL21 (DE3)-codon plus-RIL NV;WNlNmTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NFfqfHdjgSCobIXvdoV{[2WwY3WgZY5qe2:2cn;wfUBie3OjeR?= NHexbmVKSzVyIE2gNE4xPyEQvF2= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV6NkexPEc,Ojh3OE[3NVg9N2F-
22Rv1 M3fhNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MW[5OkBp MlLITWM2OCB;IECuNFcyKM7:TR?= M3rIdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
22Rv1 MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NUHqWZVmOTJiaB?= MoPmTWM2OCB;IECuNFcyKM7:TR?= NHz1PVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
MV4-11 M4O4RWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFe4c5dKSzVyIE2gNE4xPzJizszN M{HZNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
BL21(DE3)-R3-pRARE2 M4nDPWZ2dmO2aX;uJIF{e2G7 M4CycVMxKG2rboO= MnPYTWM2OCB;IECuNFc4KM7:TR?= M4iwdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
BL21(DE3)-R3-pRARE2 MXvGeY5kfGmxbjDhd5NigQ>? M3TYXVMxKG2rboO= MljOTWM2OCB;IECuNFc4KM7:TR?= MlTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzOUW3NlMoRjJ6MUm1O|I{RC:jPh?=
BL21(DE3)-R3-pRARE2 M1fGfWZ2dmO2aX;uJIF{e2G7 M4\4fVMxKG2rboO= MnXvTWM2OCB;IECuNFc4KM7:TR?= NIfYdXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe3Olg{PCd-Mkm3O|Y5OzR:L3G+
MV4-11 MVrDfZRwfG:6aXPpeJkh[XO|YYm= MViyOEBp MWPHTVUxKD1iMD6wPEDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF7MUO2N{c,OjZzOUGzOlM9N2F-
MV411 M3:xU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlHVO|IhcA>? MX7JR|UxKD1iMD6wPEDPxE1? NH7MWFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
TY82 NUD4cGxnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYnyS4Z5PzJiaB?= MmXmTWM2OCB;IECuNFgxQCEQvF2= NYTSd4pLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlU1OzVpPkK5OVI2PDN3PD;hQi=>
HL60 M1fkdWZ2dmO2aX;uJIF{e2G7 NULMO2JUOjRiaB?= NVuydnk2UUN3MDC9JFAvODh4IN88US=> M123e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
697 MVrDfZRwfG:6aXPpeJkh[XO|YYm= MkHpOUBl[Xm| NUfuWlVOTUN3MDC9JFAvODlizszN MkXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
Loucy NGDue|hEgXSxdH;4bYNqfHliYYPzZZk> NUXpcGJDPSCmYYnz MYPFR|UxKD1iMD6wPUDPxE1? NYHNPGFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
BL21(DE3) MUDGeY5kfGmxbjDhd5NigQ>? NGjVb|JM\CB;IECuNFkzKM7:TR?= M1TBPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
BL21(DE3)-R3-pRARE2 NVvOcY1tTnWwY4Tpc44h[XO|YYm= NXjXZW9TU2RiPTCwMlEh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV7NUCwO{c,Ojh3OUWwNFc9N2F-
HT-29 M1uyZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVy3NkBp MonETWM2OCB;IECuNVA1KM7:TR?= NFHNRnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1PVQzQCd-MkW1OVk1Ojh:L3G+
MM1S NXy0eItFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXO3NkBp NHX2XVBKSzVyIE2gNE4yODlizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3OUSyPEc,OjV3NUm0Nlg9N2F-
LNCAP cells M4LkeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3\w[mlEPTBiPTCwMlExQTZizszN M3XPPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
T cells MYXGeY5kfGmxbjDhd5NigQ>? MWqyOEBp M2L6eWlEPTBiPTCwMlEyKM7:TR?= NG[xZ3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
HL60 NVP5[5hoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWm3NkBp MknZTWM2OCB;IECuNVEh|ryP NGPDOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki2PVE6PCd-Mk[4OlkyQTR:L3G+
BL21(DE3) NVS3TmkzTnWwY4Tpc44h[XO|YYm= MkHjTWM2OCB;IECuNVIh|ryP M{jXNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxOFkxLz5{NkezNVQ6ODxxYU6=
BL21(DE3) NILSU3VHfW6ldHnvckBie3OjeR?= M{DXbFIvPSCq NYSxO|ZNUUN3MDC9JFAvOTJizszN M3fpXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
H1299 MnjVSpVv[3Srb36gZZN{[Xl? NEXDOYIzPCCq NFrkc5hGSzVyIE2gNE4yPTNizszN NI\xSXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0PVUzOSd-Mki5OFk2OjF:L3G+
HD-MB03 M3XiZmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{fNSlUh\GG7cx?= NF:wb2dGSzVyIE2gNE4yPiEQvF2= NWTzelRtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP NWjSbXllSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mlz1PVYhcA>? MVvJR|UxKD1iMD6xOkDPxE1? NHXISVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
Hs578T MlKzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4fLflEzKGh? M2[3eWlEPTBiPTCwMlE3KM7:TR?= M{Xv[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
MV4-11 MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MV:xNkBp MULJR|UxKD1iMD6xOkDPxE1? NVHpeWJPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxO|AxOjRpPkK5NVcxODJ2PD;hQi=>
LNCAP NU\1XXFCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{PDTVEzKGh? NGjnXIpKSzVyIE2gNE4yPiEQvF2= NH7MPFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
C4-2B MnPwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1TiV|k3KGh? MnK0TWM2OCB;IECuNVkh|ryP NF3QPZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
C4-2B NITKepVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnLKNVIhcA>? Mo\4TWM2OCB;IECuNVkh|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2MUO3NUc,Ojl3NEGzO|E9N2F-
Vero E6 MWfBcpRqfmm{YXygZYN1cX[rdImgZZN{[Xl? MXi0PEBp NYjHcXNXUUN3MDC9JFAvOTl{N{Wg{txO NXSwNG1pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{KzOVM5PTlpPkOyN|U{QDV7PD;hQi=>
MCF7 M2fuR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MoPiNVIhcA>? M3jLb2lEPTBiPTCwMlIh|ryP MnPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MV4-11 MnTqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mlj4TWM2OCB;IECuNlQh|ryP Mo\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNEK3OVEoRjJ5MUSyO|UyRC:jPh?=
MV4-11 NYX6dXpGS3m2b4TvfIlkcXS7IHHzd4F6 NIX5fm44OiCq NF\1[XhKSzVyIE2gNE4zPDJizszN NHj3cII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WxO|AyOSd-MkO1NVcxOTF:L3G+
MX1 NELJWFlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmTmO|IhcA>? M4PCPGVEPTBiPTCwMlI2PCEQvF2= NWPwOGtIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFk2OjFpPkK4PVQ6PTJzPD;hQi=>
HT-29 Mn2zRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlTOO|IhcA>? MlrvTWM2OCB;IECuNlgh|ryP MlvSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
HFL1 NVTocYtLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MmHINVIhcA>? NE\ZSGdKSzVyIE2gNE4zQSEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
MDA-MB-231 NXr5d4VHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NETHNXI{KGSjeYO= NX\SdHI6T0N3MDC9JFAvOyEQvF2= M3XEOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
MV4-11 NWfkW|g6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NILKNXQ1QCCq M1PmO2VEPTBiPTCwMlM{OTF|IN88US=> M3HnVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
K562 NHe1d3lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnHZTWM2OCB;IECuOlQh|ryP Ml36QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNEK3OVEoRjJ5MUSyO|UyRC:jPh?=
HL60 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MWe0PEBp Mo[1SWM2OCB;IECuO|QyOzFizszN MkmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUWwNFcoRjJ6NUm1NFA4RC:jPh?=
NCI-H1975 Mn71RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NH7tdHYyOiCq M17CV2lEPTBiPTCxMlI{KM7:TR?= NX7XTnpQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
SAE NGqwUlVHfW6ldHnvckBie3OjeR?= MVy0JIg> MVvJR|UxKD1iMT6zPEDPxE1? NVPMRZk{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE NWHhZ3lQTnWwY4Tpc44h[XO|YYm= M4LuTVQhcA>? NF7mfFhKSzVyIE2gNU41QSEQvF2= NWPKbZdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE NE\6ZpNHfW6ldHnvckBie3OjeR?= NXntVGJJPCCq MmPGTWM2OCB;IEGuOVEh|ryP MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
U2OS NXTHT|RNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYPYeYRTOTJiaB?= NWHNPY9HUUN3MDC9JFEvPjJizszN NX\UVWh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
SAE MlvUSpVv[3Srb36gZZN{[Xl? MnXPOEBp Mn;XTWM2OCB;IEGuOlMh|ryP NWfpO2hmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
A549 NWjNUWh5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEPhdFMyOiCq MXnJR|UxKD1iMT62O{DPxE1? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
MCF7 M{\LZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvE[JJsOyCmYYnz NWG4UlRRT0N3MDC9JFEvPyEQvF2= NFKyZoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW0PVg5QSd-Mki1OFk5QDl:L3G+
DU145 NEj0OGlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYW5OkBp NEXuVVdKSzVyIE2gNk42OiEQvF2= NXjPXHpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
DU145 NGrEXZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MV6xNkBp M4DxOWlEPTBiPTCyMlUzKM7:TR?= NV23WmR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
T47D M{TkdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoOwN{Bl[Xm| MYXHR|UxKD1iMj64JO69VQ>? Mnv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
PC3 NUfYWG8zSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXnMU|NRQTZiaB?= MlXVTWM2OCB;IEOuNFEh|ryP M2jhblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
PC3 NVS5WIxxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3ntbFEzKGh? M1XBbmlEPTBiPTCzMlAyKM7:TR?= M4DifFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|cyLz5{OUW0NVM4OTxxYU6=
HeLa NH;HUYpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mo[0NVIhcA>? M{fsOmlEPTBiPTCzMlc3KM7:TR?= NYHXTYFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
K562 NGLpdGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3PIWlMh\GG7cx?= M{fkdGdEPTBiPTCzMlgh|ryP MmS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
A2780 M4e2NGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYfpcXBlOyCmYYnz MWfHR|UxKD1iNDFOwG0> M1TNWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
HL60 NV73WXI3S3m2b4TvfIlkcXS7IHHzd4F6 Mnr3NlQhcA>? NFvpfXBKSzVyIE2gOU4{KM7:TR?= M3vFR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[2PVk6Lz5{N{K2Olk6QTxxYU6=
MV4-11 NYPocG1ZS3m2b4TvfIlkcXS7IHHzd4F6 Ml3QNlQhcA>? MnTITWM2OCB;IE[uOEDPxE1? NV3W[mdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkeyOlY6QTlpPkK3NlY3QTl7PD;hQi=>
K562 NX3jfWRTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NGLFUVA4OiCq NIS2V|BKSzVyIE2gPU4yOiEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDNzNEWxN{c,Ojh|MUS1NVM9N2F-
OVCAR5 M2PYNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1rPWVMh\GG7cx?= M1u3eWdEPTBiPTCxNkDPxE1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID