(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。

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RMB 2227.39 现货
RMB 4667.45 现货
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客户使用Selleck生产的(+)-JQ1发表文献250篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MWXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmTzNlUxNzVyMD:xNFAxKG6P NYj1WY5oOjRxNEivO|IhcA>? NVjTT5c2TE2VTx?= MnnsbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> NX;Nb4oxOjZ5MEe4PFE>
BCPAP NXXmdo06S2WubDDWbYFjcWyrdImgRZN{[Xl? MUOyOVAwPTByL{GwNFAhdk1? M3TSXlI1NzR6L{eyJIg> MXfEUXNQ M1j6S4lvcGmkaYTzJINmdGxidnnhZoltcXS7IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> NVmwWJVpOjZ5MEe4PFE>
K1  NEXNNGRE\WyuIFP5Z4xmKEG|c3H5 MUSyOVAwPTByL{GwNFAhdk1? MlPwO|IhcA>? NWf3cWp3TE2VTx?= Moi2ZZJz\XO2czDj[YxtKGO7Y3zlJIF1KEdyL1exJJBp[XOn NFvRUmQzPjdyN{i4NS=>
BCPAP NH;6eGNE\WyuIFP5Z4xmKEG|c3H5 NFv2R5QzPTBxNUCwM|ExODBibl2= MkHrO|IhcA>? MUnEUXNQ NUTrZWdb[XK{ZYP0d{Bk\WyuIHP5Z4xmKGG2IFewM2cyKHCqYYPl NXzjNGVOOjZ5MEe4PFE>
Hep3B M1rRUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV[wMVExKM7:TR?= MX61JIQ> NIrDRmVFVVOR MV7JR|UxRTBwMEig{txO NUPvVJlPOjZ3N{WxOlc>
HCCLM3 MnK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETDVpkxNTFyIN88US=> NHXiOoE2KGR? NYnO[3p6TE2VTx?= MV\JR|UxRTBwMUSg{txO MU[yOlU4PTF4Nx?=
HuH7 MlrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fo[FAuOTBizszN NUHvU2tuPSCm M33uZWROW09? MnG4TWM2OD1yLkKxJO69VQ>? NUS0[I5KOjZ3N{WxOlc>
HepG2 MnTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYWwMVExKM7:TR?= Mlm1OUBl MmPHSG1UVw>? MVXJR|UxRTBwM{Sg{txO M3q2SFI3PTd3MU[3
SMMC7721 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{THRlAuOTBizszN NXPhOWZ5PSCm NH3FXHJFVVOR NGfCdXdKSzVyPUCuOFEh|ryP MVuyOlU4PTF4Nx?=
BEL7402 NWT2UZB2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrkNE0yOCEQvF2= MnH5OUBl MXPEUXNQ M{PlXWlEPTB;MD60O{DPxE1? MljqNlY2PzVzNke=
MHCC97H MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFK5e|IxNTFyIN88US=> NIXBSlk2KGR? Ml22SG1UVw>? NEXUXGZKSzVyPUCuOFEh|ryP NV3QR|lqOjZ3N{WxOlc>
Hep3B NI\ER4tE\WyuIFP5Z4xmKEG|c3H5 MXywMlEwOC53L{KuOUDPxE1? M1fSelQ5KGh? NYn5WIpnTE2VTx?= NWKzT41FdGWjZIOgeI8h[SC|dXLzeIFvfGmjbDDhZ4N2dXWuYYTpc44hd2ZiSFPDJINmdGy|IHnuJJN2[i2JMTDwbIF{\cLi M2fTUVI3PTd3MU[3
HCCLM3 MU\D[YxtKEO7Y3zlJGF{e2G7 NIfzW5kxNjFxMD61M|IvPSEQvF2= MXG0PEBp M1nOOWROW09? MkTBcIVi\HNidH:gZUB{fWK|dHHueIlidCCjY3P1cZVt[XSrb36gc4YhUEOFIHPlcIx{KGmwIIP1Zk1IOSCyaHHz[eKh MUiyOlU4PTF4Nx?=
Hep3B NEjGNm1CeG:ydH;zbZMhSXO|YYm= NVj4OFN[OC5zL{CuOU8zNjVizszN MWe0PEBp MlLTSG1UVw>? M4rBN4FkfGm4YYTld{Bk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOTDlfJBz\XO|aX;uJIFv\CCrbnT1Z4VlKFCDUmCgZ4xm[X[jZ3WgZZMhf2WubDDhd{BkgXSxY3jyc41mKGNicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21iZoLvcUBucXSxY3jvcoRzcWF? MWSyOlU4PTF4Nx?=
HCCLM3 NWDoN3dGSXCxcITvd4l{KEG|c3H5 NIjoTIcxNjFxMD61M|IvPSEQvF2= MUi0PEBp MXLEUXNQ NX7EZ5RC[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NX60NmxkOjZ3N{WxOlc>
A549 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XDXlAvOS1zMDFOwG0> NYXCZVd{PzJiaB?= M{HQR4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NX\u[Yh1OjZ2MUWyNlU>
H157 NIL1U3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjSXVcxNjFvMUCg{txO NEDUN5o4OiCq MYXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> Mm\WNlY1OTV{MkW=
H1299 MkXIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTwNE4yNTFyIN88US=> M1zFNFczKGh? NGrwPWdqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MV[yOlQyPTJ{NR?=
A549 M4fsRWZ2dmO2aX;uJGF{e2G7 MnjFNU8zNjVxNTFOwG0> M13RUVEzKGh? MkDvxsB4\WGtbImg[IVkemWjc3XkJGJkdC1{IHzleoVtew>? NUSwbJpYOjZ2MUWyNlU>
H1299 MYPGeY5kfGmxbjDBd5NigQ>? MUCxM|IvPS93IN88US=> NE\GfG4yOiCq M4[0VuKhf2Wja3z5JIRm[3KnYYPl[EBD[2xvMjDs[ZZmdHN? MViyOlQyPTJ{NR?=
H157 NFT1WIRHfW6ldHnvckBCe3OjeR?= NUfLe3pGOS9{LkWvOUDPxE1? M2TxTlEzKGh? MXjk[YNz\WG|ZXSgSHI1KGW6cILld5Nqd25? MonMNlY1OTV{MkW=
H1299 MUnGeY5kfGmxbjDBd5NigQ>? M1jHN|EwOi53L{Wg{txO NGDCb2YyOiCq NHr6N5Vl\WO{ZXHz[YQhTFJ2IHX4dJJme3Orb36= NV:5PItCOjZ2MUWyNlU>
C8161 NHPKfY5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M3XuXVAuOiEQvF2= MUm0JIQ> Mlr6SG1UVw>? MXXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MX6yOlM6PzJ{Mx?=
Mel285 MVzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M1q5VFAuOiEQvF2= NYD1OotvPCCm MXjEUXNQ MXHk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M33LdlI3Ozl5MkKz
Mel290 NEjObnFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2jmTVAuOiEQvF2= NV;3cY5WPCCm NVTHdFU2TE2VTx?= NGO1fWJl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M1\Z[lI3Ozl5MkKz
92.1 MlHqR4VtdCCYaXHibYxqfHliQYPzZZk> Mlf4NE0zKM7:TR?= M13lZlQh\A>? MmruSG1UVw>? MY\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M4e4fFI3Ozl5MkKz
Omm1.3 NFv5dlVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXvF[lRXOC1{IN88US=> NV;ubVFiPCCm NUTiZ4hWTE2VTx?= MVvk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NVuyWZBYOjZ|OUeyNlM>
Mel202 M4\EPWNmdGxiVnnhZoltcXS7IFHzd4F6 NH3ad5YxNTJizszN MnLVOEBl M1rjWWROW09? NF;ZWGFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MkG2NlY{QTd{MkO=
Mel270 MXfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGK1UHQxNTJizszN NFHsOGE1KGR? MYHEUXNQ MlvE[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NHnsTpczPjN7N{KyNy=>
Omm1 NXvIPHN4S2WubDDWbYFjcWyrdImgRZN{[Xl? M32yXlAuOiEQvF2= NX\2XWxEPCCm NYLke2lTTE2VTx?= NI\G[nRl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MUiyOlM6PzJ{Mx?=
92.1 M2nVbWFxd3C2b4Ppd{BCe3OjeR?= NXj0W2xIPTByIH7N MUm0PEBp NFvyfFdFVVOR MWHpcoR2[2W|IHHwc5B1d3Orcx?= M4\pflI3Ozl5MkKz
Omm1.3 M{\qTWFxd3C2b4Ppd{BCe3OjeR?= NIi5bIs2ODBibl2= MlfPOFghcA>? M{e4NGROW09? MoPDbY5lfWOnczDhdI9xfG:|aYO= NEDjVpkzPjN7N{KyNy=>
92.1 NH\FRlhE\WyuIFP5Z4xmKEG|c3H5 MonXOVAxKG6P NYf1ZpAxOjRxNEivO|IhcA>? MYjEUXNQ MlPsbY5lfWOnczD0bIUh[2WubDDhZ4N2dXWuYYTpc44h[XRic4XiMWcyyqB? MWWyOlM6PzJ{Mx?=
Omm1.3 MnzTR4VtdCCFeXPs[UBCe3OjeR?= NGK4PYc2ODBibl2= M2ntT|I1NzR6L{eyJIg> NIS0cVdFVVOR NFfO[Y5qdmS3Y3XzJJRp\SClZXzsJIFk[3WvdXzheIlwdiCjdDDzeYIuTzIEoB?= NH\VTYMzPjN7N{KyNy=>
A549 NUPiO3RFTnWwY4Tpc44hSXO|YYm= M2DWOlExOC92MECvNVAxOCCwTR?= MYGyOEBp NXT2Rm4xfXC{ZXf1cIF1\XNiYX7kJIFkfGm4YYTld{BUUVKWMR?= MkLMNlYzOTJzOUm=
MCF-7 MnXISpVv[3Srb36gRZN{[Xl? MmfpNVAxNzRyMD:xNFAxKG6P MVuyOEBp NHThXmV2eHKnZ4XsZZRmeyCjbnSgZYN1cX[jdHXzJHNKWlRz NEjpco4zPjJzMkG5PS=>
HEK293 MmDFSpVv[3Srb36gRZN{[Xl? M3vaPFExOC92MECvNVAxOCCwTR?= NHG2fWIzPCCq M{TCVpVxemWpdXzheIV{KGGwZDDhZ5RqfmG2ZYOgV2lTXDF? NXfxPY1tOjZ{MUKxPVk>
858 MnTLR4VtdCCYaXHibYxqfHliQYPzZZk> NEW1c5ExNTFizszN M3\TVFUh\A>? MliwSG1UVw>? NHjkUmFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3y3VFI3OjB4M{Oz
DDR2L63V MmLDR4VtdCCYaXHibYxqfHliQYPzZZk> MYKwMVEh|ryP NEKxTm82KGR? NUDRRmJ6TE2VTx?= M3vCWYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NIHoelYzPjJyNkOzNy=>
BE(2)-C Mlv3R4VtdCCYaXHibYxqfHliQYPzZZk> MUexJO69VQ>? M3TyXVEuPCCm MWnk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlic3nncolncWOjboTsfS=> MlfhNlYxPjd2NkS=
IMR-32 M2HZSWNmdGxiVnnhZoltcXS7IFHzd4F6 NIXCfZYyKM7:TR?= MlfuNU01KGR? NWrCe2JN\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JJNq\26rZnnjZY51dHl? Ml\sNlYxPjd2NkS=
JF Mm\ER4VtdCCYaXHibYxqfHliQYPzZZk> NX;2O5F{OSEQvF2= M3HWV|EuPCCm M17hR4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDzbYdvcW[rY3HueIx6 MUiyOlA3PzR4NB?=
BE(2)-M17 M2fjS2NmdGxiVnnhZoltcXS7IFHzd4F6 NXjoNW1sOSEQvF2= NEC1PIQyNTRiZB?= Mlnq[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> Mny1NlYxPjd2NkS=
SK-N-SH MlXWR4VtdCCYaXHibYxqfHliQYPzZZk> MorqNUDPxE1? MnrsNU01KGR? MlXl[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> M2jwUFI3ODZ5NE[0
SK-N-DZ  Mnf5R4VtdCCYaXHibYxqfHliQYPzZZk> NYXSbXhvOSEQvF2= M3zjWlEuPCCm MY\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlic3nncolncWOjboTsfS=> MUKyOlA3PzR4NB?=
HMC-1.1  M3TvOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTjUmsyPS13MECwJI5O M2jZTlQ5KGh? Ml70SG1UVw>? MUfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NFXDZVczPjB3NUOwNy=>
HMC-1.2 MkL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXkOU02ODByIH7N MknyOFghcA>? NEDwVHBFVVOR MXLpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NYXrZpZkOjZyNUWzNFM>
ROSA KIT WT  NITBW2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfY[402NTVyMECgcm0> M{fyTVQ5KGh? NX\CWlVCTE2VTx?= NVLi[m1HcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MlG4NlYxPTV|MEO=
ROSA KIT D816V MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPN[5g2NTVyMECgcm0> NXjZfndZPDhiaB?= M4KwfWROW09? NXv0UVQxcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3nxdlI3ODV3M{Cz
HMC-1.1  M1XYPGFxd3C2b4Ppd{BCe3OjeR?= MWmyNFAuPTByMDDuUS=> M4X2W|Q5KGh? MmLYSG1UVw>? NV7BT2xucW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NWPXXJB{OjZyNUWzNFM>
HMC-1.2 NIL2TXpCeG:ydH;zbZMhSXO|YYm= MXmyNFAuPTByMDDuUS=> NYD2cWVWPDhiaB?= MX;EUXNQ NETUWVFqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ Ml3pNlYxPTV|MEO=
ROSA KIT WT  M4fuZWFxd3C2b4Ppd{BCe3OjeR?= MUCyNFAuPTByMDDuUS=> M4DJ[|Q5KGh? MlvNSG1UVw>? Mn7hbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MWqyOlA2PTNyMx?=
ROSA KIT D816V NHSzflVCeG:ydH;zbZMhSXO|YYm= MUeyNFAuPTByMDDuUS=> MXW0PEBp MUHEUXNQ M3rGNIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M{W0[lI3ODV3M{Cz
494H MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYTnXpRzPzJiaB?= NVXhPZUyTE2VTx?= MmfHTWM2OD1yLkGyNuKyOC5yMESg{txO NYLXSGUyOjV7NES1OlY>
493H MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV23NkBp MmHSSG1UVw>? NWT0SmpjUUN3ME2wMlA1P8LzMD6wNFkh|ryP Ml6yNlU6PDR3Nk[=
716H NX;NOXpVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVi3NkBp NULYOIR2TE2VTx?= M2\zZ2lEPTB;MD6yNVLDuTBwMEO0JO69VQ>? MYWyOVk1PDV4Nh?=
148I NEfrTHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfleJp6PzJiaB?= MorDSG1UVw>? NWm3SphLUUN3ME2wMlI5PMLzMD6wN|Uh|ryP M2Sze|I2QTR2NU[2
98Sc M37Ibmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLaZWM4OiCq MoL2SG1UVw>? Ml3aTWM2OD1yLkGxOeKyOC5yMESg{txO MXOyOVk1PDV4Nh?=
89R MkHjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjac3k4OiCq NF;VbVJFVVOR MVjJR|UxRTBwMUK2xtExNjByMzFOwG0> NFPOT3YzPTl2NEW2Oi=>
494L NYLZTFQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULqcFNCPzJiaB?= NUXqRXc4TE2VTx?= M2izXGlEPTB;MD6zNVfDuTBwMEGyJO69VQ>? MkXtNlU6PDR3Nk[=
493L NYTVRWVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vINVczKGh? MorISG1UVw>? NHjqe5pKSzVyPUCuNFUxyrFyLkCxNUDPxE1? MkDxNlU6PDR3Nk[=
148L MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYG3NkBp NWKy[4VoTE2VTx?= MmPMTWM2OD1yLkG0OuKyOC5yMUeg{txO NVrrcVVzOjV7NES1OlY>
98L MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnnO|IhcA>? NEjnWnlFVVOR MXzJR|UxRTBwM{C5xtExNjB{OTFOwG0> M2PnOFI2QTR2NU[2
OS17 NHzlfXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoezO|IhcA>? M2exd2ROW09? M3XuS2lEPTB;MD6wO|nDuTBwMECzJO69VQ>? NYLPS2R1OjV7NES1OlY>
OS9 MlixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2Lh[|czKGh? NIPicWdFVVOR NUHROpNyUUN3ME2wMlQxPsLzMD6wNlgh|ryP MUCyOVk1PDV4Nh?=
MG63 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1u3[FczKGh? Ml3CSG1UVw>? M1zBXGlEPTB;MD6xNVTDuTBwMEK1JO69VQ>? M1HyWlI2QTR2NU[2
SAOS2 NXuzVVBTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXmwUHNVPzJiaB?= MnvLSG1UVw>? M4ficmlEPTB;MD6yNVfDuTBwMECzJO69VQ>? NV\lfmV[OjV7NES1OlY>
U2OS M3[1[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLMO|IhcA>? NHq0c5VFVVOR Ml7ZTWM2OD1yLkG5POKyOC5yMEig{txO MmTqNlU6PDR3Nk[=
SJSA-1 NXHBeWRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUe3NkBp MXPEUXNQ NWHCSGp6UUN3ME2wMlExOMLzMD6wNVAh|ryP Ml;2NlU6PDR3Nk[=
494H Ml;5RZBweHSxc3nzJGF{e2G7 NHy4O|UxNjJ3L{CuOU8yNjBizszN NGL1VHAzPCCq MV7EUXNQ NVW5b3VOcW6lcnXhd4V{KGyndnXsd{Bw\iClbHXheoVlKGOjc4Dhd4UuO8Li NYPU[3o6OjV7NES1OlY>
148I M1rV[mFxd3C2b4Ppd{BCe3OjeR?= MX[wMlI2NzBwNT:xMlAh|ryP MlrTNlQhcA>? MVLEUXNQ MWrpcoNz\WG|ZYOgcIV3\Wy|IH;mJINt\WG4ZXSgZ4F{eGG|ZT2zxsA> MlvzNlU6PDR3Nk[=
OS17 MkTwRZBweHSxc3nzJGF{e2G7 MVSwMlI2NzBwNT:xMlAh|ryP MnvvNlQhcA>? NHrlbmxFVVOR NVnuOoE1cW6lcnXhd4V{KGyndnXsd{Bw\iClbHXheoVlKGOjc4Dhd4UuO8Li M1vZblI2QTR2NU[2
494H MmjFRZBweHSxc3nzJGF{e2G7 NVPQVFNWOeLCid88US=> NVPCdmpQPDhiaB?= Ml3BSG1UVw>? NVjaOYt7cW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6 NUTNU2hTOjV7NES1OlY>
148I M1HEU2Fxd3C2b4Ppd{BCe3OjeR?= MknrNgKBkc7:TR?= M{i0WVQ5KGh? NICxOGFFVVOR MkDabY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|aXfubYZq[2GwdHz5 MWiyOVk1PDV4Nh?=
OS17 MXXBdI9xfG:|aYOgRZN{[Xl? M2PLeFHjiIoQvF2= MYO0PEBp NEDiR21FVVOR MV7pcoR2[2W|IHPlcIwh[XCxcITvd4l{KHOrZ37p[olk[W62bIm= NVOxSGc3OjV7NES1OlY>
MOLM13 NHzFeJNCeG:ydH;zbZMhSXO|YYm= NVj5[ZlGOjVyIH7N M{jjcFQ5KGh? NHLTWlBFVVOR NV\1WnVjcW6mdXPld{B{cWewaX\pZ4FvfGy7IHHwc5B1d3OrczDjc5Rz\WG2bXXueEB4cXSqIIH1bZpienSrbnni NFLjSmEzPTB3M{iyOS=>
MV4-11  MWrBdI9xfG:|aYOgRZN{[Xl? NVixeVZxOjVyIH7N NEfITIo1QCCq Mor5SG1UVw>? MX;pcoR2[2W|IIPp[45q\mmlYX70cJkh[XCxcITvd4l{KGOxdILlZZRu\W62IIfpeIgheXWrenHyeIlvcWJ? M1K4SFI2ODV|OEK1
MOLM13 NEToOlBHfW6ldHnvckBCe3OjeR?= M{PBeFI2OCCwTR?= Mn\ENlQhcA>? Mn25SG1UVw>? Mo\X[Y5p[W6lZYOgdZVqgmG{dHnubYIucW6mdXPl[EBud3KnIICyNUwhSkmPLDDhcoQh[2ynYY\l[EBRSVKS NYDMW49XOjVyNUO4NlU>
MV4-11  M3nNdWZ2dmO2aX;uJGF{e2G7 NHjvfXMzPTBibl2= Mn6wNlQhcA>? Mk\kSG1UVw>? NEPxW2FmdmijbnPld{ByfWm8YYL0bY5q[i2rbnT1Z4VlKG2xcnWgdFIyNCCESV2sJIFv\CClbHXheoVlKFCDUmC= NF3KWW0zPTB3M{iyOS=>
MOLM13 MkfYRZBweHSxc3nzJGF{e2G7 MoXVNlUxKG6P MYG0PEBp NYTzZXRrTE2VTx?= NHPUdodqdmS3Y3XzJJNq\26rZnnjZY51dHliYYDvdJRwe2m|IHPveJJm[XSvZX70JJdqfGhicH;uZZRqdmmk NIn1OYQzPTB3M{iyOS=>
MV4-11  MV3BdI9xfG:|aYOgRZN{[Xl? NFvrXXczPTBibl2= NF7UW5A1QCCq MnfxSG1UVw>? NF2yVndqdmS3Y3XzJJNq\26rZnnjZY51dHliYYDvdJRwe2m|IHPveJJm[XSvZX70JJdqfGhicH;uZZRqdmmk MYeyOVA2Ozh{NR?=
Hela NHLvVnNE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M{LHbVAuPTByIH7N NUfW[HJoPzJiaB?= MnfWSG1UVw>? MYfk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M37aPVI2ODB7Mkm1
HBL-1 M4W0cmNmdGxiVnnhZoltcXS7IFHzd4F6 M1fC[|AuPTByIH7N MXW3NkBp NVLwO2lGTE2VTx?= NUHDOIRK\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MkDPNlUxODl{OUW=
HLY-1 MoLSR4VtdCCYaXHibYxqfHliQYPzZZk> MljtNE02ODBibl2= MlPzO|IhcA>? MXHEUXNQ NHO4[Ipl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3TuSVI2ODB7Mkm1
OCI-Ly3 MnvXR4VtdCCYaXHibYxqfHliQYPzZZk> NYr1VWhuOC13MECgcm0> MnroO|IhcA>? MX3EUXNQ NFm2WG5l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NXPQdIhNOjVyMEmyPVU>
OCI-Ly10 NXO3WllTS2WubDDWbYFjcWyrdImgRZN{[Xl? MortNE02ODBibl2= MoLDO|IhcA>? NVXaWopVTE2VTx?= NFLNUXpl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NH7jd4gzPTByOUK5OS=>
SU-DHL-4 MWrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NFvHN5cxNTVyMDDuUS=> M3Lwd|czKGh? MnezSG1UVw>? M37FSoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MoH2NlUxODl{OUW=
SU-DHL-5 NIP2UVJE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NHHZblExNTVyMDDuUS=> NXTPTJFHPzJiaB?= NGjvXY5FVVOR NUPHTYY3\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NIC0SHEzPTByOUK5OS=>
SU-DHL-6 MWrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmjDNE02ODBibl2= NF3ycpM4OiCq MmnkSG1UVw>? MoC2[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NEWxXo0zPTByOUK5OS=>
SU-DHL-10 NF32[I5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NUfp[WJTOC13MECgcm0> M3G3Z|czKGh? MVnEUXNQ M{D0UoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MXeyOVAxQTJ7NR?=
RC-K8 MX7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MlTVNE02ODBibl2= NEP6NnU4OiCq M4qybGROW09? NWrmPGNW\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MUmyOVAxQTJ7NR?=
OCI-Ly8 NUnUZYRHS2WubDDWbYFjcWyrdImgRZN{[Xl? MnS0NE02ODBibl2= NWnWcHF3PzJiaB?= M33a[GROW09? NUPROpJu\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NFLKOWozPTByOUK5OS=>
OCL-Ly18 NV24VmxwS2WubDDWbYFjcWyrdImgRZN{[Xl? Mne5NE02ODBibl2= MYC3NkBp NXPpNXUxTE2VTx?= NVO3S|N2\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M4fDSFI2ODB7Mkm1
OCI-Ly3 MljOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLtNVczNzJ3MD:1NFAhdk1? MYqyM|ch\A>? M{TIWmROW09? NEnDeYZqdmS3Y3XzJINmdGxvY4njcIUh[XK{ZYP0JIF1KHO3Yj3HNUB4cXSqIH3pcolu[WxiY3XscEBl\WG2aNMg NIjHcHYzPTByOUK5OS=>
OCI-Ly8 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVqxO|IwOjVyL{WwNEBvVQ>? NGPMNY4zNzdiZB?= NILPZYNFVVOR MnHJbY5lfWOnczDj[YxtNWO7Y3zlJIFzemW|dDDheEB{fWJvR{Gge4l1cCCvaX7pcYFtKGOnbHyg[IVifGkEoB?= NH3YbI4zPTByOUK5OS=>
SU-DHL-4 NG\EZmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELTPHcyPzJxMkWwM|UxOCCwTR?= MoH4Nk84KGR? MlnoSG1UVw>? MlvBbY5lfWOnczDj[YxtNWO7Y3zlJIFzemW|dDDheEB{fWJvR{Gge4l1cCCvaX7pcYFtKGOnbHyg[IVifGkEoB?= MYeyOVAxQTJ7NR?=
SU-DHL-10 NXniOWJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLkNVczNzJ3MD:1NFAhdk1? NVvlRVluOi95IHS= MVfEUXNQ NWXHcGhwcW6mdXPld{Bk\WyuLXP5Z4xmKGG{cnXzeEBifCC|dXKtS|Ehf2m2aDDtbY5qdWGuIHPlcIwh\GWjdHlCpC=> MWWyOVAxQTJ7NR?=
OCI-Ly3 NIfIc4tCeG:ydH;zbZMhSXO|YYm= MmTKNVczNzJ3MDDuUS=> MX23[C=> MmX2SG1UVw>? NES3XW1qdmO{ZXHz[ZMh[2G|cHHz[U0{NzdiYXP0bZZqfHoEoIPp[45q\mmlYX70cJk> MUiyOVAxQTJ7NR?=
OCI-Ly8 MkTURZBweHSxc3nzJGF{e2G7 Mkm4NVczNzJ3MDDuUS=> NYPEdHFxP2R? NWDabZNYTE2VTx?= NGntd2ZqdmO{ZXHz[ZMh[2G|cHHz[U0{NzdiYXP0bZZqfHoEoIPp[45q\mmlYX70cJk> M1zpbVI2ODB7Mkm1
SU-DHL-4 NHrKd3hCeG:ydH;zbZMhSXO|YYm= M4HtTlE4Oi9{NUCgcm0> MXG3[C=> NXzle4hNTE2VTx?= MoLBbY5kemWjc3XzJINie3Cjc3WtN{84KGGldHn2bZR6yqC|aXfubYZq[2GwdHz5 MVqyOVAxQTJ7NR?=
SU-DHL-10 MlrORZBweHSxc3nzJGF{e2G7 M2TqdVE4Oi9{NUCgcm0> MnG1O4Q> NFe0VHlFVVOR M1rFS4lv[3KnYYPld{Bk[XOyYYPlMVMwPyCjY4Tpeol1gcLic3nncolncWOjboTsfS=> NUHySWNuOjVyMEmyPVU>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Ethanol 91 mg/mL (199.12 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID