(+)-JQ1

For research use only. Not for use in humans.

目录号:S7110

(+)-JQ1 Chemical Structure

CAS No. 1268524-70-4

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1695.33 现货
RMB 2227.39 现货
RMB 4667.45 现货
RMB 11220.4 现货
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客户使用Selleck生产的(+)-JQ1发表文献261篇:

产品安全说明书

Target Protein Ligand抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。(+)-JQ1可通过诱导自噬来抑制细胞增殖。(+)-JQ1 可抑制 Nuclear receptor binding SET domain protein 3 (NSD3) 的靶基因表达。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  MlH3R4VtdCCYaXHibYxqfHliQYPzZZk> MVKyOVAwPTByL{GwNFAhdk1? NVzsW2R4OjRxNEivO|IhcA>? NHzHTZJFVVOR NEn3eoxqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz M3yxe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
BCPAP MYnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NVrqeHFHOjVyL{WwNE8yODByIH7N MYqyOE81QC95MjDo NIj4VHRFVVOR Mm\6bY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> NFznflU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewO|g5OSd-Mk[3NFc5QDF:L3G+
K1  MnjnR4VtdCCFeXPs[UBCe3OjeR?= MkTQNlUxNzVyMD:xNFAxKG6P MYO3NkBp MWjEUXNQ MY\hdpJme3S|IHPlcIwh[3mlbHWgZZQhTzBxR{GgdIhie2V? NUfDflZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFc5QDFpPkK2O|A4QDhzPD;hQi=>
BCPAP NU\lRW12S2WubDDDfYNt\SCDc4PhfS=> M4rsNVI2OC93MECvNVAxOCCwTR?= NIH6[oU4OiCq MoGwSG1UVw>? NXnoS2lp[XK{ZYP0d{Bk\WyuIHP5Z4xmKGG2IFewM2cyKHCqYYPl M2D5[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{C3PFgyLz5{NkewO|g5OTxxYU6=
Hep3B M3P6eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4O2elAuOTBizszN Mkj0OUBl NXH4c|NRTE2VTx?= MVfJR|UxRTBwMEig{txO NUjZZ4RvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
HCCLM3 M4fqT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\DcXB4OC1zMDFOwG0> M3vVXlUh\A>? MXjEUXNQ NIHzfllKSzVyPUCuNVQh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HuH7 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVywMVExKM7:TR?= NVz5VGpUPSCm MXfEUXNQ NGTsZWFKSzVyPUCuNlEh|ryP M1G5eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUe1NVY4Lz5{NkW3OVE3PzxxYU6=
HepG2 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfXNE0yOCEQvF2= MX61JIQ> NYXIZYZCTE2VTx?= M{f0NWlEPTB;MD6zOEDPxE1? MlTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
SMMC7721 Mkj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHhTGpZOC1zMDFOwG0> NVWzT4lYPSCm MWfEUXNQ NYXEdHhvUUN3ME2wMlQyKM7:TR?= NWfnW5h{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1O|UyPjdpPkK2OVc2OTZ5PD;hQi=>
BEL7402 M1;DRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4S4UlAuOTBizszN NF3GZY02KGR? NFvCN3lFVVOR NF7ydmFKSzVyPUCuOFch|ryP Ml;hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3N{WxOlcoRjJ4NUe1NVY4RC:jPh?=
MHCC97H M17iWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlKwNE0yOCEQvF2= NFvZV5c2KGR? NFfFOVRFVVOR M3vZXmlEPTB;MD60NUDPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
Hep3B MUnD[YxtKEO7Y3zlJGF{e2G7 M3[4[FAvOS9yLkWvNk42KM7:TR?= MV:0PEBp MknESG1UVw>? NHn4bJRt\WGmczD0c{BiKHO3YoP0ZY51cWGuIHHjZ5VufWyjdHnvckBw\iCKQ1OgZ4VtdHNiaX6gd5VjNUdzIIDoZZNmyqB? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
HCCLM3 NFjRVWRE\WyuIFP5Z4xmKEG|c3H5 NGPlWZExNjFxMD61M|IvPSEQvF2= NXjJVY8yPDhiaB?= MVzEUXNQ MorZcIVi\HNidH:gZUB{fWK|dHHueIlidCCjY3P1cZVt[XSrb36gc4YhUEOFIHPlcIx{KGmwIIP1Zk1IOSCyaHHz[eKh MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5NUG2O{c,OjZ3N{WxOlc9N2F-
Hep3B MWnBdI9xfG:|aYOgRZN{[Xl? M{PKfFAvOS9yLkWvNk42KM7:TR?= NXP3[ZRqPDhiaB?= NYfqR2g1TE2VTx?= NEO0Toti[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj NEX0Ook9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
HCCLM3 MUHBdI9xfG:|aYOgRZN{[Xl? M1jRSlAvOS9yLkWvNk42KM7:TR?= M1HFWFQ5KGh? MXfEUXNQ NVXIcnVl[WO2aY\heIV{KGOjc4Dhd4UuOyCjbnSgZ4F{eGG|ZT25JIV5eHKnc4Ppc44h[W6mIHnu[JVk\WRiUFHSVEBkdGWjdnHn[UBieyC5ZXzsJIF{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHnueI8hfGinIHP5eI9xdGG|bTDmdo9uKG2rdH;jbI9v\HKrYR?= NIjrPXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW3OVE3Pyd-Mk[1O|UyPjd:L3G+
A549 M2nEPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkS4NE4yNTFyIN88US=> NFrBV404OiCq NILvRmNqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MnXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H157 NGPzNlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUCwMlEuOTBizszN NYXW[HZ7PzJiaB?= MoDFbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MkH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 M4LP[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jmbFAvOS1zMDFOwG0> Mm[5O|IhcA>? NULLSm1XcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
A549 NWPl[203TnWwY4Tpc44hSXO|YYm= MVyxM|IvPS93IN88US=> MYKxNkBp NXK1UVFXyqC5ZXHrcJkh\GWlcnXhd4VlKEKlbD2yJIxmfmWucx?= M2iwXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEG1NlI2Lz5{NkSxOVIzPTxxYU6=
H1299 M{jyWmZ2dmO2aX;uJGF{e2G7 MnzkNU8zNjVxNTFOwG0> MXKxNkBp MVhCpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRzNUKyOUc,OjZ2MUWyNlU9N2F-
H157 Mle1SpVv[3Srb36gRZN{[Xl? NX;EVm0zOS9{LkWvOUDPxE1? M2PZTlEzKGh? MXvk[YNz\WG|ZXSgSHI1KGW6cILld5Nqd25? MmC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
H1299 MXnGeY5kfGmxbjDBd5NigQ>? NF3YXWIyNzJwNT:1JO69VQ>? M1m0XVEzKGh? MmjU[IVkemWjc3XkJGRTPCCneIDy[ZN{cW:w MorZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MUWyNlUoRjJ4NEG1NlI2RC:jPh?=
C8161 Mn\QR4VtdCCYaXHibYxqfHliQYPzZZk> MmfBNE0zKM7:TR?= MlXyOEBl Mmn6SG1UVw>? NIW5ZYRl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NFvRTIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel285 MoPvR4VtdCCYaXHibYxqfHliQYPzZZk> NVTHNnN{OC1{IN88US=> MUS0JIQ> NH\ae|hFVVOR MkfN[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NEO3b2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel290 MoTrR4VtdCCYaXHibYxqfHliQYPzZZk> M{POe|AuOiEQvF2= M1rOclQh\A>? MkTBSG1UVw>? NH;qd|Jl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MkG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
92.1 M1uzVmNmdGxiVnnhZoltcXS7IFHzd4F6 M2rFOlAuOiEQvF2= M2PjZ|Qh\A>? Mm\ZSG1UVw>? MUDk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NVr1eFFlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPVczOjNpPkK2N|k4OjJ|PD;hQi=>
Omm1.3 NF\QfGpE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NVTqeZY{OC1{IN88US=> MnWzOEBl MnPGSG1UVw>? NGnweHdl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= Mlf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|OUeyNlMoRjJ4M{m3NlI{RC:jPh?=
Mel202 MYHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnrINE0zKM7:TR?= MYG0JIQ> MkThSG1UVw>? NITFRpJl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NITZOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO5O|IzOyd-Mk[zPVczOjN:L3G+
Mel270 NXr0bFlxS2WubDDWbYFjcWyrdImgRZN{[Xl? NFnhN|cxNTJizszN NWfp[Wl4PCCm NVHnT5E4TE2VTx?= MVzk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M{jxflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1 NIi4NmpE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWTvV3FQOC1{IN88US=> M{nE[|Qh\A>? NEDKUXFFVVOR MVXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M1viTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
92.1 M33zNGFxd3C2b4Ppd{BCe3OjeR?= MnXMOVAxKG6P MnzIOFghcA>? NVe3RpRjTE2VTx?= M2HFcIlv\HWlZYOgZZBweHSxc3nz M{XSdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{m3NlI{Lz5{NkO5O|IzOzxxYU6=
Omm1.3 MnT2RZBweHSxc3nzJGF{e2G7 NEH6VoU2ODBibl2= M3zCbVQ5KGh? MkTFSG1UVw>? NVvnS2h5cW6mdXPld{BieG:ydH;zbZM> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN7N{KyN{c,OjZ|OUeyNlM9N2F-
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MV4-11  NH;oXFlCeG:ydH;zbZMhSXO|YYm= MU[yOVAhdk1? M3HEXVQ5KGh? NH7CTZdFVVOR MV7pcoR2[2W|IIPp[45q\mmlYX70cJkh[XCxcITvd4l{KGOxdILlZZRu\W62IIfpeIgheXWrenHyeIlvcWJ? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB3M{iyOUc,OjVyNUO4NlU9N2F-
MOLM13 MoPjSpVv[3Srb36gRZN{[Xl? NEXnWpkzPTBibl2= NF3PXFUzPCCq NXfxfnVDTE2VTx?= Ml;x[Y5p[W6lZYOgdZVqgmG{dHnubYIucW6mdXPl[EBud3KnIICyNUwhSkmPLDDhcoQh[2ynYY\l[EBRSVKS M1m2SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEWzPFI2Lz5{NUC1N|gzPTxxYU6=
MV4-11  MXzGeY5kfGmxbjDBd5NigQ>? MYeyOVAhdk1? NHrFUZQzPCCq Mn7lSG1UVw>? NFLCdFlmdmijbnPld{ByfWm8YYL0bY5q[i2rbnT1Z4VlKG2xcnWgdFIyNCCESV2sJIFv\CClbHXheoVlKFCDUmC= NIDSWHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC1N|gzPSd-MkWwOVM5OjV:L3G+
MOLM13 Mni1RZBweHSxc3nzJGF{e2G7 MYiyOVAhdk1? NH2xUmQ1QCCq NXjOW3RWTE2VTx?= NGfTWIdqdmS3Y3XzJJNq\26rZnnjZY51dHliYYDvdJRwe2m|IHPveJJm[XSvZX70JJdqfGhicH;uZZRqdmmk NFrtU489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC1N|gzPSd-MkWwOVM5OjV:L3G+
MV4-11  NHeyOo9CeG:ydH;zbZMhSXO|YYm= NUTXNmNKOjVyIH7N MVq0PEBp MXfEUXNQ NXj6XmJPcW6mdXPld{B{cWewaX\pZ4FvfGy7IHHwc5B1d3OrczDjc5Rz\WG2bXXueEB4cXSqIIDvcoF1cW6rYh?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB3M{iyOUc,OjVyNUO4NlU9N2F-
Hela MXnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2[3S|AuPTByIH7N M33GelczKGh? NWj4PWN3TE2VTx?= M4fzS4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NWK0SGhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFkzQTVpPkK1NFA6Ojl3PD;hQi=>
HBL-1 NEXtSmdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M12xclAuPTByIH7N MknUO|IhcA>? NX\SUoUyTE2VTx?= M2j0N4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M3\2[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEC5Nlk2Lz5{NUCwPVI6PTxxYU6=
HLY-1 NFrvc|JE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MmrnNE02ODBibl2= NHq2XVM4OiCq MoHNSG1UVw>? NYqwUVJj\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2HucVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEC5Nlk2Lz5{NUCwPVI6PTxxYU6=
OCI-Ly3 MXjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NEDpcGoxNTVyMDDuUS=> M2OybFczKGh? NWXCZ2NvTE2VTx?= MX7k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NXXQc5NMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFkzQTVpPkK1NFA6Ojl3PD;hQi=>
OCI-Ly10 MVPD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mnu1NE02ODBibl2= M3PVU|czKGh? M4foeGROW09? M3rzbIRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOUK5OUc,OjVyMEmyPVU9N2F-
SU-DHL-4 M121R2NmdGxiVnnhZoltcXS7IFHzd4F6 NH;ScG8xNTVyMDDuUS=> M1;zUlczKGh? MkP3SG1UVw>? NYPxU40z\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOUK5OUc,OjVyMEmyPVU9N2F-
SU-DHL-5 NHTVfXRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUKwMVUxOCCwTR?= MYe3NkBp NHTXdGNFVVOR NIj2dmNl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= Mo[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
SU-DHL-6 NHzRSndE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXzydpl[OC13MECgcm0> MV[3NkBp NIHlfWtFVVOR NVuwPJZ6\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MnH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
SU-DHL-10 NXi5PI9wS2WubDDWbYFjcWyrdImgRZN{[Xl? M4O3S|AuPTByIH7N MWW3NkBp MUfEUXNQ MUXk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NGq0Vlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPVI6PSd-MkWwNFkzQTV:L3G+
RC-K8 MWLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVmwMVUxOCCwTR?= NGqw[lI4OiCq NGLyXolFVVOR MVTk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NXKxfVFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNFkzQTVpPkK1NFA6Ojl3PD;hQi=>
OCI-Ly8 MWjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmTZNE02ODBibl2= MW[3NkBp MkDQSG1UVw>? M2q4VYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MnnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
OCL-Ly18 NX75V2l7S2WubDDWbYFjcWyrdImgRZN{[Xl? NI\xdVgxNTVyMDDuUS=> MV23NkBp M13nOGROW09? NYLOfpoz\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Mn:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
OCI-Ly3 MlrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M333eVE4Oi9{NUCvOVAxKG6P MmruNk84KGR? NUTPTZJITE2VTx?= NGT2VJNqdmS3Y3XzJINmdGxvY4njcIUh[XK{ZYP0JIF1KHO3Yj3HNUB4cXSqIH3pcolu[WxiY3XscEBl\WG2aNMg MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOUK5OUc,OjVyMEmyPVU9N2F-
OCI-Ly8 M3zNN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;VVlE4Oi9{NUCvOVAxKG6P M{Pqd|IwPyCm MULEUXNQ NGjwbYtqdmS3Y3XzJINmdGxvY4njcIUh[XK{ZYP0JIF1KHO3Yj3HNUB4cXSqIH3pcolu[WxiY3XscEBl\WG2aNMg MnnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
SU-DHL-4 NEXCTWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTINVczNzJ3MD:1NFAhdk1? MkX4Nk84KGR? NXnTOFFUTE2VTx?= MXHpcoR2[2W|IHPlcIwu[3mlbHWgZZJz\XO2IHH0JJN2[i2JMTD3bZRpKG2rbnntZYwh[2WubDDk[YF1cMLi MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTByOUK5OUc,OjVyMEmyPVU9N2F-
SU-DHL-10 NF;TUWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DrU|E4Oi9{NUCvOVAxKG6P MXKyM|ch\A>? MlX0SG1UVw>? MnKzbY5lfWOnczDj[YxtNWO7Y3zlJIFzemW|dDDheEB{fWJvR{Gge4l1cCCvaX7pcYFtKGOnbHyg[IVifGkEoB?= Ml\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEmyPVUoRjJ3MEC5Nlk2RC:jPh?=
OCI-Ly3 M4LEV2Fxd3C2b4Ppd{BCe3OjeR?= MoSzNVczNzJ3MDDuUS=> NIP6Z|Q4\A>? MW\EUXNQ MlTDbY5kemWjc3XzJINie3Cjc3WtN{84KGGldHn2bZR6yqC|aXfubYZq[2GwdHz5 M3ruSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEC5Nlk2Lz5{NUCwPVI6PTxxYU6=
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SU-DHL-4 NUDvN|NkSXCxcITvd4l{KEG|c3H5 Mlm2NVczNzJ3MDDuUS=> MXm3[C=> M3z2SGROW09? M1jReolv[3KnYYPld{Bk[XOyYYPlMVMwPyCjY4Tpeol1gcLic3nncolncWOjboTsfS=> NITKdmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPVI6PSd-MkWwNFkzQTV:L3G+
SU-DHL-10 M4O5bmFxd3C2b4Ppd{BCe3OjeR?= NXizVW9yOTd{L{K1NEBvVQ>? MV23[C=> NHjhbVhFVVOR MWrpcoNz\WG|ZYOgZ4F{eGG|ZT2zM|ch[WO2aY\peJnDqHOrZ37p[olk[W62bIm= NHrXc209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwPVI6PSd-MkWwNFkzQTV:L3G+
Rosetta2 DE3 M{XjXGZ2dmO2aX;uJIF{e2G7 NVfTVWh6U2RiPTCwMlAxPjJizszN NVzPdpkzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 MnvkSox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? NUfRcXhOOzBibXnudy=> M3PXT2tqKD1iMD6wNFY3KM7:TR?= NEnyW3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 NVHiNGN7TnWwY4Tpc44h[XO|YYm= MX;L[EA:KDBwMEC2O{DPxE1? NGnubIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 NEnBbJBHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 NWCyS|ljOzBibXnudy=> NG\vNIlMcSB;IECuNFA4PiEQvF2= M2XPdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 NGHGXmpHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 MoXwN|AhdWmwcx?= NWnXbY1oU2liPTCwMlAxQDlizszN NUfKOINVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NITZcolHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 MkK0N|AhdWmwcx?= Ml3IT4khRSByLkCxNFch|ryP MmXtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOECwOlQoRjJ4MEiwNFY1RC:jPh?=
Rosetta2 DE3 M2W1cGZ2dmO2aX;uJIF{e2G7 NUCzdVJ3U2RiPTCwMlAyOTdizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
MV4-11 MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWrMVo04PzJiaB?= Mn\uTWM2OCB;IECuNFEzKM7:TR?= NEfDV209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkezNVQ6OCd-Mk[3N|E1QTB:L3G+
Rosetta2 DE3 MWTGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MX2zNEBucW6| MnrsT4khRSByLkCxNkDPxE1? NUTG[ZZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
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Rosetta2 DE3 Mnu4Sox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigQ>? MnnDN|AhdWmwcx?= M3XrVmtqKD1iMD6wNVI2KM7:TR?= M13aZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 MlrVSpVv[3Srb36gZZN{[Xl? NEC0OI5M\CB;IECuNFEzQCEQvF2= NIPadXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
Rosetta2 DE3 MXPGcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6 MX2zNEBucW6| MYTLbUA:KDBwMEGzNkDPxE1? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 MXvGeY5kfGmxbjDhd5NigQ>? NF24NXRM\CB;IECuNFE{PiEQvF2= M{\6b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 M4XkSGZ2dmO2aX;uJIF{e2G7 NYnr[XJWU2RiPTCwMlAyPDdizszN MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MEC2OEc,OjZyOECwOlQ9N2F-
Rosetta2 DE3 NHywc3JHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 NWThfHhMOzBibXnudy=> MnztT4khRSByLkCxOFkh|ryP Ml7rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
TY82 NV;1UZB6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NGr1VGI4OiCq NIPOPWtKSzVyIE2gNE4xOThizszN NWHhbINTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PFY4OThpPkK4OVg3PzF6PD;hQi=>
MM1S NIHTOpBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUm3NkBp NW\jSnFCUUN3MDC9JFAvODF7IN88US=> NGfrRZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlcyQCd-Mki1PFY4OTh:L3G+
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HT-29 NGLxUm5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NX3u[3FrOTJiaB?= MmO2TWM2OCB;IECuNFIh|ryP Mkm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3M{WwOFUoRjJ6NUO1NFQ2RC:jPh?=
MV4-11 Mk\zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYG3NkBp M4LrOGlEPTBiPTCwMlAzOyEQvF2= M4m0clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW5OFI5Lz5{NUW1PVQzQDxxYU6=
MV4-11 M2ToR2N6fG:2b4jpZ4l1gSCjc4PhfS=> NUTMPHRvPCCmYYnz MYrJR|UxKD1iMD6wNlQh|ryP NYXMTHRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
MV4-11 M37hNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVL5[mt{PCCmYYnz M1PDfWlEPTBiPTCwMlAzPCEQvF2= M1LheFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
Rosetta2 DE3 MWHGeY5kfGmxbjDhd5NigQ>? M{CxSlMxKG2rboO= MoO4TWM2OCB;IECuNFI5PyEQvF2= M{HpTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEiwNFY1Lz5{NkC4NFA3PDxxYU6=
NALM16 MnOxR5l1d3SxeHnjbZR6KGG|c3H5 NIr4WGw2KGSjeYO= NUXUdW1iTUN3MDC9JFAvODNizszN M{m4clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUewNFI1Lz5{OUG3NFAzPDxxYU6=
BL21(DE3)-R3-pRARE2 MYrGeY5kfGmxbjDhd5NigQ>? MWOzNEBucW6| NEW2XWJKSzVyIE2gNE4xOzNizszN M4LK[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O|I{Lz5{OEG5OVczOzxxYU6=
BL21(DE3) M1;P[WZ2dmO2aX;uJIF{e2G7 MYLL[EA:KDBwMEO0JO69VQ>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd|MUS5NEc,OjZ5M{G0PVA9N2F-
Rosetta2 DE3 NGPyOZRHdHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7 M4mxZlMxKG2rboO= MWLJR|UxKD1iMD6wN|U4KM7:TR?= NUC3fol6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFAxPjRpPkK2NFgxODZ2PD;hQi=>
Rosetta2 DE3 NVvQNJpkTmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NUnCUYtvOzBibXnudy=> M4jNT2lEPTBiPTCwMlA1OjJizszN NVTmdoZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OlM1QDdpPkK4OFY{PDh5PD;hQi=>
Rosetta2 DE3 M4PPb2ZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gZZN{[Xl? M2fMTFMxKG2rboO= MUDJR|UxKD1iMD6wOFY4KM7:TR?= Mn23QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NkO0PFcoRjJ6NE[zOFg4RC:jPh?=
MOLM13 MW\DfZRwfG:6aXPpeJkh[XO|YYm= Mlu0OEBl[Xm| NW\tNoZQUUN3MDC9JFAvODV4IN88US=> NGLhNGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2N|Q5Pyd-Mki0OlM1QDd:L3G+
MOLM13 MlrQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3W0cVQh\GG7cx?= NX3vfFliUUN3MDC9JFAvODV4IN88US=> NI\zOHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NFA3PCd-Mk[wPFAxPjR:L3G+
HL60 NWL1OW04SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHy4cJo4OiCq NVrN[49mUUN3MDC9JFAvODZizszN NF3Kfmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
HL60 NYnQcYs1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHPqelk{KGSjeYO= MVHHR|UxKD1iMD6wOkDPxE1? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3N{C5PUc,Ojl4NUewPVk9N2F-
NALM6 M3G1UWN6fG:2b4jpZ4l1gSCjc4PhfS=> NH3BV3k2KGSjeYO= NXy2dVVlTUN3MDC9JFAvODZizszN M4rTSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
Raji M4r2dWZ2dmO2aX;uJIF{e2G7 NVmxVWZtUUN3MDC9JFAvODZizszN MnO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{G0PVAoRjJ4N{OxOFkxRC:jPh?=
Raji M4D6S2Z2dmO2aX;uJIF{e2G7 NGHMNm81KGh? NXTSRm51UUN3MDC9JFAvODZ7IN88US=> NIHtSpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFc2QCd-MkS5NFA4PTh:L3G+
MM1S NWfuU4NkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYfUbIgyPzJiaB?= MV3JR|UxKD1iMD6wOlkyKM7:TR?= NVLQTW1ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlU1OzVpPkK5OVI2PDN3PD;hQi=>
BL21 (DE3)-codon plus-RIL NVLxS2RETmy3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeR?= NF;WclJjgSCobIXvdoV{[2WwY3WgZY5qe2:2cn;wfUBie3OjeR?= NFzwT45KSzVyIE2gNE4xPyEQvF2= M3XJdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUi2O|E5Lz5{OEW4OlcyQDxxYU6=
22Rv1 NH3jb45CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NXj4e4hKQTZiaB?= M2LnW2lEPTBiPTCwMlA4OSEQvF2= NVKzN3ZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
22Rv1 Ml3aRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NEP0UoMyOiCq NHrGVI5KSzVyIE2gNE4xPzFizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2MUO3NUc,Ojl3NEGzO|E9N2F-
MV4-11 NUDpO5R3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NI\vTYtKSzVyIE2gNE4xPzJizszN Mni5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzOUW3NlMoRjJ6MUm1O|I{RC:jPh?=
BL21(DE3)-R3-pRARE2 NYHoTXl3TnWwY4Tpc44h[XO|YYm= Moq5N|AhdWmwcx?= M3THSmlEPTBiPTCwMlA4PyEQvF2= NXrKVIJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3)-R3-pRARE2 NIDRSolHfW6ldHnvckBie3OjeR?= NXrqfo1wOzBibXnudy=> NX3PO45IUUN3MDC9JFAvODd5IN88US=> NH;Beo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
BL21(DE3)-R3-pRARE2 MoixSpVv[3Srb36gZZN{[Xl? M{\WTFMxKG2rboO= NEGwNFlKSzVyIE2gNE4xPzdizszN M3nBRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{e2PFM1Lz5{OUe3Olg{PDxxYU6=
MV4-11 NWraUW9ZS3m2b4TvfIlkcXS7IHHzd4F6 NHmzSG0zPCCq NWH3PVBpT0l3MDC9JFAvODhizszN NYrFXINiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVE{PjNpPkK2NVkyOzZ|PD;hQi=>
MV411 NFrjPWJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NILiT|k4OiCq MWrJR|UxKD1iMD6wPEDPxE1? MknVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|MUS1NVMoRjJ6M{G0OVE{RC:jPh?=
TY82 NX7mZ4xXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NFXCWlY4OiCq NXq5XZROUUN3MDC9JFAvODhyODFOwG0> NFjtVms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOVQ{PSd-Mkm1NlU1OzV:L3G+
HL60 M3LwS2Z2dmO2aX;uJIF{e2G7 M1zPWlI1KGh? MlHsTWM2OCB;IECuNFg3KM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
697 M1XU[mN6fG:2b4jpZ4l1gSCjc4PhfS=> M1TOVVUh\GG7cx?= NUC1enpiTUN3MDC9JFAvODlizszN MmGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
Loucy M2HMTWN6fG:2b4jpZ4l1gSCjc4PhfS=> NFzDZmM2KGSjeYO= NEHMU41GSzVyIE2gNE4xQSEQvF2= MoDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzN{CwNlQoRjJ7MUewNFI1RC:jPh?=
BL21(DE3) M2\2bmZ2dmO2aX;uJIF{e2G7 MoTFT4QhRSByLkC5NkDPxE1? NWHwUHI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
BL21(DE3)-R3-pRARE2 M4j0fGZ2dmO2aX;uJIF{e2G7 MWTL[EA:KDBwMTFOwG0> M4HxfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
HT-29 NIfOV5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV:1UIIyPzJiaB?= NF\FeI5KSzVyIE2gNE4yODRizszN NWjuWYpLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
MM1S MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkHmO|IhcA>? MXjJR|UxKD1iMD6xNFkh|ryP NX\qcHVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVk1OjhpPkK1OVU6PDJ6PD;hQi=>
LNCAP cells NWmxWFhiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mo\RTWM2OCB;IECuNVA6PiEQvF2= NYG3ZoV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
T cells M{X5ZWZ2dmO2aX;uJIF{e2G7 MWeyOEBp NYrPV3piUUN3MDC9JFAvOTFizszN NHLB[lM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEOxOFUyOyd-MkizNVQ2OTN:L3G+
HL60 NGfxZnVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M1PR[lczKGh? NWjMWVA5UUN3MDC9JFAvOTFizszN M{\rWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OE[5NVk1Lz5{Nki2PVE6PDxxYU6=
BL21(DE3) NGXoWVhHfW6ldHnvckBie3OjeR?= NUDEcoJXUUN3MDC9JFAvOTJizszN NXnzcYpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
BL21(DE3) NHrSemJHfW6ldHnvckBie3OjeR?= MkHsNk42KGh? NFT2dVZKSzVyIE2gNE4yOiEQvF2= MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzO|EoRjJ7NUSxN|cyRC:jPh?=
H1299 MmHxSpVv[3Srb36gZZN{[Xl? MnzmNlQhcA>? NHTmcYpGSzVyIE2gNE4yPTNizszN MlLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEm1NlEoRjJ6OUS5OVIyRC:jPh?=
HD-MB03 NYjmWnN6S3m2b4TvfIlkcXS7IHHzd4F6 NVLkVHlDPSCmYYnz NX3rWW1ITUN3MDC9JFAvOTZizszN NXzNOolZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OVg2OThpPkK5O|U5PTF6PD;hQi=>
LNCAP Mo[2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVW5OkBp NVjYdHU2UUN3MDC9JFAvOTZizszN M4PpelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
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MV4-11 Moj6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M16yeVEzKGh? MYfJR|UxKD1iMD6xOkDPxE1? NGrWPIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG3NFAzPCd-MkmxO|AxOjR:L3G+
LNCAP M4rQNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYWxNkBp M1zhbmlEPTBiPTCwMlE3KM7:TR?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2MUO3NUc,Ojl3NEGzO|E9N2F-
C4-2B MmDoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYm5OkBp MnPlTWM2OCB;IECuNVkh|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
C4-2B NWHrbG5DSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1ntS|EzKGh? NIjZVlZKSzVyIE2gNE4yQSEQvF2= NEnqTmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
Vero E6 MmHWRY51cX[rcnHsJIFkfGm4aYT5JIF{e2G7 MlPaOFghcA>? NEn6UVVKSzVyIE2gNE4yQTJ5NTFOwG0> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OjN3M{i1PUc,OzJ|NUO4OVk9N2F-
MCF7 Ml31RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NEnucIEyOiCq NFXwVWlKSzVyIE2gNE4zKM7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
MV4-11 M2S4eWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUDRfmdYUUN3MDC9JFAvOjRizszN M3LleVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUSyO|UyLz5{N{G0Nlc2OTxxYU6=
MV4-11 MUTDfZRwfG:6aXPpeJkh[XO|YYm= M1n4VlczKGh? NWH6Vm1PUUN3MDC9JFAvOjR{IN88US=> NXfJXoVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVcxOTFpPkKzOVE4ODFzPD;hQi=>
MX1 NHmzXIdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHzhVFk4OiCq M4\sNmVEPTBiPTCwMlI2PCEQvF2= NWTHb|NbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFk2OjFpPkK4PVQ6PTJzPD;hQi=>
HT-29 MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2DMfVczKGh? NU\JVIVrUUN3MDC9JFAvOjhizszN NUT6emRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E1QTBpPkK2O|MyPDlyPD;hQi=>
HFL1 MnTCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFzu[nAyOiCq M1:5e2lEPTBiPTCwMlI6KM7:TR?= MoX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
MDA-MB-231 NXO0Zms{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXezJIRigXN? M3\BUWdEPTBiPTCwMlMh|ryP NUXUW3llRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OFk5QDlpPkK4OVQ6QDh7PD;hQi=>
MV4-11 MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MWq0PEBp NVHNVYdVTUN3MDC9JFAvOzNzMUOg{txO M2T1SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUm1NFA4Lz5{OEW5OVAxPzxxYU6=
K562 M{\ocGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NVS3S4lGUUN3MDC9JFAvPjRizszN NGD2b2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G0Nlc2OSd-MkexOFI4PTF:L3G+
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NCI-H1975 NYrLXJFxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{XoSFEzKGh? M2e3TWlEPTBiPTCxMlI{KM7:TR?= M1iwVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
SAE MWDGeY5kfGmxbjDhd5NigQ>? MVO0JIg> NYDafnhmUUN3MDC9JFEvOzhizszN NX7UZllERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFk4PDFpPkK5OlQ6PzRzPD;hQi=>
SAE MVPGeY5kfGmxbjDhd5NigQ>? NU\PfIpKPCCq MoLjTWM2OCB;IEGuOFkh|ryP M1e4VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkS5O|QyLz5{OU[0PVc1OTxxYU6=
SAE NGPz[YdHfW6ldHnvckBie3OjeR?= NGLST3U1KGh? NYfiSpJ6UUN3MDC9JFEvPTFizszN MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2OUe0NUc,Ojl4NEm3OFE9N2F-
U2OS NXyxS5Z7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWGxNkBp MlfoTWM2OCB;IEGuOlIh|ryP MluzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5NUi1NVgoRjJ7N{W4OVE5RC:jPh?=
SAE NXfq[mV1TnWwY4Tpc44h[XO|YYm= NF3sPW41KGh? M2e1ZWlEPTBiPTCxMlY{KM7:TR?= M{W1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkS5O|QyLz5{OU[0PVc1OTxxYU6=
A549 M1u4d2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWK5OJJvOTJiaB?= NILzW3FKSzVyIE2gNU43PyEQvF2= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
MCF7 NHG5[XlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUDIb45KOyCmYYnz NX;Ib4lyT0N3MDC9JFEvPyEQvF2= Mnz5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=
DU145 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MVy5OkBp MmXETWM2OCB;IEKuOVIh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd3OEWxPEc,Ojl5NUi1NVg9N2F-
DU145 Ml;iRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXWxNkBp M3fPSGlEPTBiPTCyMlUzKM7:TR?= NFrnZYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM4OSd-Mkm1OFE{PzF:L3G+
T47D NHLLcHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVWzJIRigXN? NULofJlwT0N3MDC9JFIvQCEQvF2= M2r6VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUS5PFg6Lz5{OEW0PVg5QTxxYU6=
PC3 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NInqSWM6PiCq NWHBZplRUUN3MDC9JFMvODFizszN NIf6bGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe1PFUyQCd-Mkm3OVg2OTh:L3G+
PC3 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mm\wNVIhcA>? MoG1TWM2OCB;IEOuNFEh|ryP NUi4UHZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PzFpPkK5OVQyOzdzPD;hQi=>
HeLa NIn5UHVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MWqxNkBp NXXh[GVYUUN3MDC9JFMvPzZizszN M37hfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{W4OVE5Lz5{OUe1PFUyQDxxYU6=
K562 NUny[5dQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXu4RmtPOyCmYYnz Moj0S2M2OCB;IEOuPEDPxE1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
A2780 M4DHbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NITZTIY{KGSjeYO= NH;zPXdISzVyIE2gOEDPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV2OUi4PUc,Ojh3NEm4PFk9N2F-
HL60 MnP0R5l1d3SxeHnjbZR6KGG|c3H5 NWG0R|k2OjRiaB?= MlnnTWM2OCB;IEWuN{DPxE1? Mn7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Nk[5PVkoRjJ5Mk[2PVk6RC:jPh?=
MV4-11 MWjDfZRwfG:6aXPpeJkh[XO|YYm= NG[yNoszPCCq NHX5c|RKSzVyIE2gOk41KM7:TR?= M1vvWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[2PVk6Lz5{N{K2Olk6QTxxYU6=
K562 M1TOUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVy3NkBp MoPYTWM2OCB;IEmuNVIh|ryP M1[2U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6M{G0OVE{Lz5{OEOxOFUyOzxxYU6=
OVCAR5 NVPxfJpqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHHZUWY{KGSjeYO= Mnm5S2M2OCB;IEGyJO69VQ>? Mmf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NEm4PFkoRjJ6NUS5PFg6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Water Insoluble
Ethanol '91 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Target Protein Ligand Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID