(+)-JQ1

目录号:S7110

(+)-JQ1 Chemical Structure

Molecular Weight(MW): 456.99

(+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。

规格 价格 库存 购买数量  
RMB 2227.39 现货
RMB 4667.45 现货
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客户使用Selleck该产品发表文献42篇:

产品安全说明书

Epigenetic Reader Domain抑制剂选择性比较

生物活性

产品描述 (+)-JQ1是一种BET bromodomain抑制剂,作用于BRD4(1/2),无细胞试验中IC50为77 nM/33 nM,结合到BET家族的所有溴结构域,而不结合到BET家族以外的溴结构域。
特性 (+)-JQ1比(-)-JQ1更有效。
靶点
BRD4 (2) [1]
(Cell-free assay)
BRD4 (1) [1]
(Cell-free assay)
33 nM 77 nM
体外研究

(+)-JQ1对映体直接结合到BET bromodomain结构域的Kac结合位点。(+)-JQ1(500 nM)与染色质竞争性结合到BRD4,导致NMC细胞分化和生长停滞。通过Ki67染色减少,证明了(+)-JQ1(500 nM)减弱NMC 797和Per403细胞系的快速增殖。(+)-JQ1(500 nM)作用于NMC 797细胞,有效降低BRD4靶基因的表达。(+)-JQ1作用于NMC 11060细胞,抑制细胞活力, IC50为4 nM。[1](+)-JQ1作用于MM细胞系,强抑制MYC表达。(+)-JQ1抑制KMS-34和LR5增殖,IC50分别为 68 nM和98 nM。(+)-JQ1(500 nM)处理MM.1S细胞,导致S期细胞比例明显下降,随之细胞停滞在G0/G1期增多。(+)-JQ1(500 nM)通过β-半乳糖苷酶染色,导致明显的细胞衰老。(+)-JQ1(800 nM)处理CD138+病患衍生的MM样本,显著降低细胞活力。[2] (+)-JQ1抑制LP-1细胞生长,GI50 为98 nM。(+)-JQ1(625 nM)导致LP-1细胞在G0/G1期的百分数增高。(+)-JQ1(500 nM)作用于LP-1细胞,抑制MYC, BRD4 和CDK9表达。[3](+)-JQ1(1 μM)处理潜伏感染的Jurkat T细胞,激活HIV转录。(+)-JQ1(50 μM)作用于Jurkat和HeLa细胞,主要刺激Tat依赖性的HIV转录。(+)-JQ1(5 μM)作用于J-Lat A2细胞,诱导Brd4解离,从而使Tat招募SEC到HIV启动子上,诱导Pol II CTD磷酸化和病毒转录。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K1  NF\RTHhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1XJ[VI2OC93MECvNVAxOCCwTR?= MXmyOE81QC95MjDo MXXEUXNQ MVnpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy NWnISnNJOjZ5MEe4PFE>
BCPAP MlTlR4VtdCCYaXHibYxqfHliQYPzZZk> NX7lVlVROjVyL{WwNE8yODByIH7N NGPXSVIzPC92OD:3NkBp NVywUIN6TE2VTx?= NXPoR5pbcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgbY4h[m:2aDDkc5NmNSCjbnSgeIlu\S1iZHXw[Y5l\W62IH3hco5meg>? Mn;3NlY4ODd6OEG=
K1  NED5NIZE\WyuIFP5Z4xmKEG|c3H5 NVXLZWNTOjVyL{WwNE8yODByIH7N MmPnO|IhcA>? NI\Q[WJFVVOR MlvxZZJz\XO2czDj[YxtKGO7Y3zlJIF1KEdyL1exJJBp[XOn NVP6eGJ[OjZ5MEe4PFE>
BCPAP Mo\pR4VtdCCFeXPs[UBCe3OjeR?= MoPmNlUxNzVyMD:xNFAxKG6P MV23NkBp NVf0XnN4TE2VTx?= NGHseWFienKnc4TzJINmdGxiY4njcIUh[XRiR{CvS|EheGijc3W= NUWyNZlxOjZ5MEe4PFE>
Hep3B NVzUeotnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoW2NE0yOCEQvF2= NV7OdnZQPSCm MV;EUXNQ MU\JR|UxRTBwMEig{txO NF33eJozPjV5NUG2Oy=>
HCCLM3 M1fMTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrqTo0xNTFyIN88US=> NYDRRZp3PSCm NVnXepd5TE2VTx?= NXLYXJFrUUN3ME2wMlE1KM7:TR?= M4PTelI3PTd3MU[3
HuH7 NUXobmtkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWWwMVExKM7:TR?= MUK1JIQ> NVXleWNrTE2VTx?= NE\0PHVKSzVyPUCuNlEh|ryP MV:yOlU4PTF4Nx?=
HepG2 M2f1PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHmcZQ2OC1zMDFOwG0> MYK1JIQ> MUjEUXNQ NYPuTJExUUN3ME2wMlM1KM7:TR?= MkLYNlY2PzVzNke=
SMMC7721 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDke4QzOC1zMDFOwG0> NW\qN4tFPSCm NHrU[lNFVVOR MYrJR|UxRTBwNEGg{txO MV[yOlU4PTF4Nx?=
BEL7402 NXjDOZdiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLNfGZCOC1zMDFOwG0> MYm1JIQ> MmK2SG1UVw>? NFHYZlNKSzVyPUCuOFch|ryP NWr0dlZkOjZ3N{WxOlc>
MHCC97H Mkn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjYNE0yOCEQvF2= MoSwOUBl NGDyXZpFVVOR M1rHNmlEPTB;MD60NUDPxE1? MkPnNlY2PzVzNke=
Hep3B NX32TWNQS2WubDDDfYNt\SCDc4PhfS=> M1GyVVAvOS9yLkWvNk42KM7:TR?= M3mzPFQ5KGh? M4PZfmROW09? MojjcIVi\HNidH:gZUB{fWK|dHHueIlidCCjY3P1cZVt[XSrb36gc4YhUEOFIHPlcIx{KGmwIIP1Zk1IOSCyaHHz[eKh MYeyOlU4PTF4Nx?=
HCCLM3 M{j4OGNmdGxiQ4njcIUhSXO|YYm= NXPDeJd[OC5zL{CuOU8zNjVizszN NFjLbnU1QCCq NUjQZ3hQTE2VTx?= MnfxcIVi\HNidH:gZUB{fWK|dHHueIlidCCjY3P1cZVt[XSrb36gc4YhUEOFIHPlcIx{KGmwIIP1Zk1IOSCyaHHz[eKh MnfmNlY2PzVzNke=
Hep3B NGGyO49CeG:ydH;zbZMhSXO|YYm= NETDOVQxNjFxMD61M|IvPSEQvF2= Ml7HOFghcA>? M3vEN2ROW09? NIXiVnli[3SrdnH0[ZMh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUmg[ZhxemW|c3nvckBidmRiaX7keYNm\CCSQWLQJINt\WG4YXflJIF{KHenbHygZZMh[3m2b3Podo9u\SClIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOvIH\yc40hdWm2b3Poc45lemmj NE\2dY8zPjV5NUG2Oy=>
HCCLM3 MXXBdI9xfG:|aYOgRZN{[Xl? NVnXTHZVOC5zL{CuOU8zNjVizszN M3q4T|Q5KGh? MXjEUXNQ MnHXZYN1cX[jdHXzJINie3Cjc3WtN{BidmRiY3HzdIF{\S17IHX4dJJme3Orb36gZY5lKGmwZIXj[YQhWEGUUDDjcIVifmGpZTDhd{B4\WyuIHHzJIN6fG:laILvcYUh[yC{ZXzlZZNmKGmwdH:geIhmKGO7dH;wcIF{dSCocn;tJI1qfG:laH;u[JJq[Q>? M2TzPVI3PTd3MU[3
A549 MnXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELCd2kxNjFvMUCg{txO NF33cVg4OiCq MVzpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NWXve3FsOjZ2MUWyNlU>
H157 M1O2eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nrbVAvOS1zMDFOwG0> NEnYSZM4OiCq M1X3eIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M1HLdFI3PDF3MkK1
H1299 M3PDemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUGwMlEuOTBizszN M3TLO|czKGh? MWjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NXXBe|ZKOjZ2MUWyNlU>
A549 MlLqSpVv[3Srb36gRZN{[Xl? NXq3PWhIOS9{LkWvOUDPxE1? NFvTcYYyOiCq MV1CpJdm[WuueTDk[YNz\WG|ZXSgRoNtNTJibHX2[Yx{ M3jKZlI3PDF3MkK1
H1299 M3TJRWZ2dmO2aX;uJGF{e2G7 MUixM|IvPS93IN88US=> MXWxNkBp M1Lp[OKhf2Wja3z5JIRm[3KnYYPl[EBD[2xvMjDs[ZZmdHN? MXWyOlQyPTJ{NR?=
H157 NGDFc|JHfW6ldHnvckBCe3OjeR?= MX[xM|IvPS93IN88US=> Mlq2NVIhcA>? M{TNfoRm[3KnYYPl[EBFWjRiZYjwdoV{e2mxbh?= NYK5XoFKOjZ2MUWyNlU>
H1299 M{XqR2Z2dmO2aX;uJGF{e2G7 MXSxM|IvPS93IN88US=> MYqxNkBp MVXk[YNz\WG|ZXSgSHI1KGW6cILld5Nqd25? M3SxSVI3PDF3MkK1
C8161 NVXwe3RDS2WubDDWbYFjcWyrdImgRZN{[Xl? MoXyNE0zKM7:TR?= M{XQTlQh\A>? M1;hSWROW09? NIXNOVll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M1;qZVI3Ozl5MkKz
Mel285 NEC5SnZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MkPLNE0zKM7:TR?= NEHLPGs1KGR? NUjFWolRTE2VTx?= MWjk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NIfKOJQzPjN7N{KyNy=>
Mel290 NYPncG1uS2WubDDWbYFjcWyrdImgRZN{[Xl? NYHWdII3OC1{IN88US=> NUDBW2cxPCCm MXHEUXNQ MV\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NUHn[Xd[OjZ|OUeyNlM>
92.1 NHHH[IFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWfSPXdtOC1{IN88US=> NFXXbHg1KGR? M4TGRWROW09? M1vKb4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NVyxWHl5OjZ|OUeyNlM>
Omm1.3 MV7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXGwMVIh|ryP NVPyOVRkPCCm MnS1SG1UVw>? NEDteYVl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NHG4TI4zPjN7N{KyNy=>
Mel202 MoDnR4VtdCCYaXHibYxqfHliQYPzZZk> NY\0U3VMOC1{IN88US=> NFO1e5Y1KGR? NFjjTFZFVVOR MV7k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M1fTTlI3Ozl5MkKz
Mel270 NF;KTW9E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2m5NFAuOiEQvF2= NV3KSVdpPCCm M2PPe2ROW09? Mlfs[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M3[4V|I3Ozl5MkKz
Omm1 NYDxRlNMS2WubDDWbYFjcWyrdImgRZN{[Xl? NVfwS3l1OC1{IN88US=> NHK3ZlY1KGR? NUX6e211TE2VTx?= NX3lVFhE\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYOyOlM6PzJ{Mx?=
92.1 MYjBdI9xfG:|aYOgRZN{[Xl? MVO1NFAhdk1? M2XSUVQ5KGh? M3fXNGROW09? NI\CPFVqdmS3Y3XzJIFxd3C2b4Ppdy=> M{PGOlI3Ozl5MkKz
Omm1.3 MXjBdI9xfG:|aYOgRZN{[Xl? NV\rR5JtPTByIH7N MUK0PEBp NUfzcoNkTE2VTx?= M1fobYlv\HWlZYOgZZBweHSxc3nz MmjtNlY{QTd{MkO=
92.1 Ml71R4VtdCCFeXPs[UBCe3OjeR?= M2C4bFUxOCCwTR?= M2KyOFI1NzR6L{eyJIg> MmSySG1UVw>? NVe1XHc1cW6mdXPld{B1cGViY3XscEBi[2O3bYXsZZRqd25iYYSgd5VjNUdzwrC= NU\WdYt5OjZ|OUeyNlM>
Omm1.3 M2jQT2NmdGxiQ4njcIUhSXO|YYm= MkKwOVAxKG6P MmDqNlQwPDhxN{KgbC=> M1nJbmROW09? NWLBZlNpcW6mdXPld{B1cGViY3XscEBi[2O3bYXsZZRqd25iYYSgd5VjNUdzwrC= MVOyOlM6PzJ{Mx?=
A549 MkPVSpVv[3Srb36gRZN{[Xl? NFn1T40yODBxNECwM|ExODBibl2= NG\mdVIzPCCq NH;qdYJ2eHKnZ4XsZZRmeyCjbnSgZYN1cX[jdHXzJHNKWlRz Mk\LNlYzOTJzOUm=
MCF-7 NX;PdoRLTnWwY4Tpc44hSXO|YYm= MW[xNFAwPDByL{GwNFAhdk1? NWHwUWd3OjRiaB?= M16wbpVxemWpdXzheIV{KGGwZDDhZ5RqfmG2ZYOgV2lTXDF? MWSyOlIyOjF7OR?=
HEK293 M2[0TWZ2dmO2aX;uJGF{e2G7 MWqxNFAwPDByL{GwNFAhdk1? M{fwelI1KGh? MV;1dJJm\3WuYYTld{BidmRiYXP0bZZifGW|IGPJVnQy MVSyOlIyOjF7OR?=
858 M3nQT2NmdGxiVnnhZoltcXS7IFHzd4F6 NWP3fXYyOC1zIN88US=> MWi1JIQ> M2rVfGROW09? NHHBcZll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M{jpNFI3OjB4M{Oz
DDR2L63V M4rueWNmdGxiVnnhZoltcXS7IFHzd4F6 NWO3PIZKOC1zIN88US=> NFH1UmU2KGR? NWnhTppNTE2VTx?= MUPk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MUOyOlIxPjN|Mx?=
BE(2)-C NGH1cJlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? Mkj5NUDPxE1? NULCVFZPOS12IHS= MULk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlic3nncolncWOjboTsfS=> MmnSNlYxPjd2NkS=
IMR-32 MWfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Ml3DNUDPxE1? MVKxMVQh\A>? Mor3[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> NX6z[pVtOjZyNke0OlQ>
JF MkS2R4VtdCCYaXHibYxqfHliQYPzZZk> MXixJO69VQ>? NXr1eIc{OS12IHS= NYLDN5V2\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JJNq\26rZnnjZY51dHl? MXyyOlA3PzR4NB?=
BE(2)-M17 M4KxWmNmdGxiVnnhZoltcXS7IFHzd4F6 NVjkdI9LOSEQvF2= MnHTNU01KGR? NEj6NpFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? MUOyOlA3PzR4NB?=
SK-N-SH MorER4VtdCCYaXHibYxqfHliQYPzZZk> NYfQU4dnOSEQvF2= NHSxepAyNTRiZB?= MkS5[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> NWOx[2I{OjZyNke0OlQ>
SK-N-DZ  Mnf1R4VtdCCYaXHibYxqfHliQYPzZZk> MVGxJO69VQ>? NYnLWpJoOS12IHS= NELJ[Fll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? MnrVNlYxPjd2NkS=
HMC-1.1  MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXu1MVUxODBibl2= NXjRbJV{PDhiaB?= M37D[2ROW09? M1rHc4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M{D5eVI3ODV3M{Cz
HMC-1.2 NIDsU5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{ezT|UuPTByMDDuUS=> NVvYcHZ4PDhiaB?= NX[4Wo1QTE2VTx?= M3fGfolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NVjEWIJUOjZyNUWzNFM>
ROSA KIT WT  MljRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojlOU02ODByIH7N MmW2OFghcA>? MWrEUXNQ M3fxTolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M1y2XFI3ODV3M{Cz
ROSA KIT D816V MnnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPjNG82NTVyMECgcm0> MYK0PEBp MmrNSG1UVw>? M3;MWIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NHLXPGgzPjB3NUOwNy=>
HMC-1.1  NFG4[olCeG:ydH;zbZMhSXO|YYm= MXqyNFAuPTByMDDuUS=> MmTlOFghcA>? M4fHUmROW09? M4fjdIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NF\kZYkzPjB3NUOwNy=>
HMC-1.2 NWDNOXVISXCxcITvd4l{KEG|c3H5 NFLTXpozODBvNUCwNEBvVQ>? MUG0PEBp NFvWT4NFVVOR NX7sO3QxcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MkPoNlYxPTV|MEO=
ROSA KIT WT  MUHBdI9xfG:|aYOgRZN{[Xl? M13NdVIxOC13MECwJI5O M3PZflQ5KGh? MnfTSG1UVw>? NHezU29qdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NVjkbplNOjZyNUWzNFM>
ROSA KIT D816V MWnBdI9xfG:|aYOgRZN{[Xl? MVmyNFAuPTByMDDuUS=> NELnfIc1QCCq NG\LSVdFVVOR M3;weIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NUjTcoluOjZyNUWzNFM>
494H M3TwZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGT2WWM4OiCq NGnhUFlFVVOR NHTRcJRKSzVyPUCuNVIzyrFyLkCwOEDPxE1? NF7VUYczPTl2NEW2Oi=>
493H M3vVeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV23NkBp MX\EUXNQ M1XWOGlEPTB;MD6wOFfDuTBwMEC5JO69VQ>? NXjKUGs1OjV7NES1OlY>
716H Mn7RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\tTodGPzJiaB?= MYfEUXNQ NFj2coxKSzVyPUCuNlEzyrFyLkCzOEDPxE1? NE\2e2gzPTl2NEW2Oi=>
148I MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7NRmR7PzJiaB?= MVTEUXNQ NEfoRXpKSzVyPUCuNlg1yrFyLkCzOUDPxE1? M1H0flI2QTR2NU[2
98Sc MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYKySnhVPzJiaB?= NYLkXGNHTE2VTx?= NHK0SXFKSzVyPUCuNVE2yrFyLkCwOEDPxE1? NXfEWGtQOjV7NES1OlY>
89R NX6wXG5RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LqVVczKGh? M4XFeWROW09? MVzJR|UxRTBwMUK2xtExNjByMzFOwG0> NIDicogzPTl2NEW2Oi=>
494L MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnpOm04OiCq MnruSG1UVw>? M1;ZXGlEPTB;MD6zNVfDuTBwMEGyJO69VQ>? NU\VSIx3OjV7NES1OlY>
493L NHvve3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkm1O|IhcA>? NEnZ[JlFVVOR NH;SSodKSzVyPUCuNFUxyrFyLkCxNUDPxE1? M{TMblI2QTR2NU[2
148L M1n5O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrjO|IhcA>? MkjlSG1UVw>? NITlXoZKSzVyPUCuNVQ3yrFyLkCxO{DPxE1? MVmyOVk1PDV4Nh?=
98L MonzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvlW211PzJiaB?= MUPEUXNQ NIP2XY5KSzVyPUCuN|A6yrFyLkCyPUDPxE1? NX\qdpk{OjV7NES1OlY>
OS17 M1Pwe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWq3NkBp NIewOZNFVVOR MWrJR|UxRTBwMEe5xtExNjByMzFOwG0> NU\lZ3FvOjV7NES1OlY>
OS9 M3rLfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDNO|IhcA>? MlrVSG1UVw>? M4DMNGlEPTB;MD60NFbDuTBwMEK4JO69VQ>? NUj0NW9vOjV7NES1OlY>
MG63 MnrLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUe3NkBp Ml7sSG1UVw>? MkT5TWM2OD1yLkGxOOKyOC5yMkWg{txO MXiyOVk1PDV4Nh?=
SAOS2 M4\OfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVWxN29ZPzJiaB?= NHfWWWJFVVOR NEXhfpNKSzVyPUCuNlE4yrFyLkCwN{DPxE1? MWCyOVk1PDV4Nh?=
U2OS MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPVVWY4OiCq NIS0cmpFVVOR MVfJR|UxRTBwMUm4xtExNjByODFOwG0> MlPhNlU6PDR3Nk[=
SJSA-1 MmXIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX:3NkBp NWq3U2xtTE2VTx?= M{DBbWlEPTB;MD6xNFDDuTBwMEGwJO69VQ>? NXX1O2tvOjV7NES1OlY>
494H NFexWmlCeG:ydH;zbZMhSXO|YYm= NWTwR4ZROC5{NT:wMlUwOS5yIN88US=> NGH4Vm8zPCCq NH34N2tFVVOR MmnjbY5kemWjc3XzJIxmfmWuczDv[kBkdGWjdnXkJINie3Cjc3WtN:Kh NHz4TY8zPTl2NEW2Oi=>
148I MWDBdI9xfG:|aYOgRZN{[Xl? M2TjZ|AvOjVxMD61M|EvOCEQvF2= NIK5WJQzPCCq M2f4O2ROW09? MUnpcoNz\WG|ZYOgcIV3\Wy|IH;mJINt\WG4ZXSgZ4F{eGG|ZT2zxsA> MViyOVk1PDV4Nh?=
OS17 NGqweoFCeG:ydH;zbZMhSXO|YYm= M2nMXFAvOjVxMD61M|EvOCEQvF2= NF3oWGwzPCCq NWrJco1ETE2VTx?= M2XwOIlv[3KnYYPld{Bt\X[nbIOgc4Yh[2ynYY\l[EBk[XOyYYPlMVPDqA>? NXe3fGRYOjV7NES1OlY>
494H M4PNOWFxd3C2b4Ppd{BCe3OjeR?= NGfI[Iky6oDLzszN NVPDSlN1PDhiaB?= Mo\OSG1UVw>? Ml\ObY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|aXfubYZq[2GwdHz5 M4O0OVI2QTR2NU[2
148I MVTBdI9xfG:|aYOgRZN{[Xl? NVW3eYsyOeLCid88US=> NEDwPXg1QCCq NFTR[oxFVVOR MYDpcoR2[2W|IHPlcIwh[XCxcITvd4l{KHOrZ37p[olk[W62bIm= MUKyOVk1PDV4Nh?=
OS17 Ml\VRZBweHSxc3nzJGF{e2G7 MkiwNgKBkc7:TR?= MYi0PEBp NFviT2pFVVOR M1raU4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3nncolncWOjboTsfS=> Mn3oNlU6PDR3Nk[=
MOLM13 NHKycmFCeG:ydH;zbZMhSXO|YYm= MknRNlUxKG6P MmjBOFghcA>? MXjEUXNQ MoPpbY5lfWOnczDzbYdvcW[rY3HueIx6KGGyb4D0c5NqeyClb4Ty[YF1dWWwdDD3bZRpKHG3aYrhdpRqdmmk M1W0c|I2ODV|OEK1
MV4-11  Ml[wRZBweHSxc3nzJGF{e2G7 NULJN4x[OjVyIH7N MYW0PEBp M3;hSWROW09? NVzVdm5LcW6mdXPld{B{cWewaX\pZ4FvfGy7IHHwc5B1d3OrczDjc5Rz\WG2bXXueEB4cXSqIIH1bZpienSrbnni M1v0dlI2ODV|OEK1
MOLM13 MmXZSpVv[3Srb36gRZN{[Xl? NWC3Nld6OjVyIH7N MWmyOEBp Mn7PSG1UVw>? NFPtWndmdmijbnPld{ByfWm8YYL0bY5q[i2rbnT1Z4VlKG2xcnWgdFIyNCCESV2sJIFv\CClbHXheoVlKFCDUmC= NGH4OYwzPTB3M{iyOS=>
MV4-11  M1zEc2Z2dmO2aX;uJGF{e2G7 NH3LUlczPTBibl2= NILHTWYzPCCq M4DTZmROW09? M2jSfYVvcGGwY3XzJJF2cXqjcoTpcoljNWmwZIXj[YQhdW:{ZTDwNlEtKEKLTTygZY5lKGOuZXH2[YQhWEGUUB?= M1vqfFI2ODV|OEK1
MOLM13 NXO2fJVXSXCxcITvd4l{KEG|c3H5 Mk\oNlUxKG6P NIfwUIk1QCCq MUTEUXNQ M4PzZ4lv\HWlZYOgd4lodmmoaXPhcpRtgSCjcH;weI9{cXNiY3;0doVifG2nboSge4l1cCCyb37heIlvcWJ? NXv1fXN2OjVyNUO4NlU>
MV4-11  MWjBdI9xfG:|aYOgRZN{[Xl? M1LkeFI2OCCwTR?= MWK0PEBp MXnEUXNQ MUPpcoR2[2W|IIPp[45q\mmlYX70cJkh[XCxcITvd4l{KGOxdILlZZRu\W62IIfpeIgheG:wYYTpcolj NX3UeYpWOjVyNUO4NlU>
Hela M3rKWWNmdGxiVnnhZoltcXS7IFHzd4F6 M1r5dVAuPTByIH7N MWe3NkBp M1SydWROW09? Mn7l[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MVqyOVAxQTJ7NR?=
HBL-1 MWXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGPC[5UxNTVyMDDuUS=> MVm3NkBp NXHLN5hpTE2VTx?= MVPk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M{DTVVI2ODB7Mkm1
HLY-1 NI\U[GdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2XkOFAuPTByIH7N NY\KfIRlPzJiaB?= MlPqSG1UVw>? Mkns[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M3LBbFI2ODB7Mkm1
OCI-Ly3 MWDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M4\ERVAuPTByIH7N NH\jXHU4OiCq NH30UmlFVVOR M4K3[YRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MmPqNlUxODl{OUW=
OCI-Ly10 MVHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NEfGXXoxNTVyMDDuUS=> NV7yT41oPzJiaB?= MmLESG1UVw>? M2XDfYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M3XKUFI2ODB7Mkm1
SU-DHL-4 NFz3XZRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MV6wMVUxOCCwTR?= MnHzO|IhcA>? Ml65SG1UVw>? MmXD[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M{nHNFI2ODB7Mkm1
SU-DHL-5 MVrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NXG4SWRDOC13MECgcm0> NY\LN2RNPzJiaB?= Mn3pSG1UVw>? NGfYR5Bl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MVmyOVAxQTJ7NR?=
SU-DHL-6 MlHsR4VtdCCYaXHibYxqfHliQYPzZZk> MnP4NE02ODBibl2= MVy3NkBp MXvEUXNQ M2X6b4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MVGyOVAxQTJ7NR?=
SU-DHL-10 M3HJZmNmdGxiVnnhZoltcXS7IFHzd4F6 NUniRoZLOC13MECgcm0> M2G2eVczKGh? MmPtSG1UVw>? NWTiPGJv\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M{fsXFI2ODB7Mkm1
RC-K8 MonzR4VtdCCYaXHibYxqfHliQYPzZZk> MXmwMVUxOCCwTR?= MX63NkBp MmDjSG1UVw>? M2r0boRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NWm1NIw1OjVyMEmyPVU>
OCI-Ly8 M3PLdmNmdGxiVnnhZoltcXS7IFHzd4F6 MYCwMVUxOCCwTR?= NIiwW|E4OiCq NY\VRZhiTE2VTx?= MoX1[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NXTrfZhROjVyMEmyPVU>
OCL-Ly18 NWX0No5JS2WubDDWbYFjcWyrdImgRZN{[Xl? MmjYNE02ODBibl2= M2DNRVczKGh? M3y3eWROW09? MnPS[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M3HUTVI2ODB7Mkm1
OCI-Ly3 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUOxO|IwOjVyL{WwNEBvVQ>? NF3hOHMzNzdiZB?= MmTYSG1UVw>? Ml7jbY5lfWOnczDj[YxtNWO7Y3zlJIFzemW|dDDheEB{fWJvR{Gge4l1cCCvaX7pcYFtKGOnbHyg[IVifGkEoB?= NXfVeFNoOjVyMEmyPVU>
OCI-Ly8 NHzSdWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXWwelAzOTd{L{K1NE82ODBibl2= NV\Qd|hyOi95IHS= MYjEUXNQ MVXpcoR2[2W|IHPlcIwu[3mlbHWgZZJz\XO2IHH0JJN2[i2JMTD3bZRpKG2rbnntZYwh[2WubDDk[YF1cMLi NF;idHozPTByOUK5OS=>
SU-DHL-4 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIKwd2syPzJxMkWwM|UxOCCwTR?= NWjQSplvOi95IHS= MVPEUXNQ M3XHW4lv\HWlZYOgZ4VtdC2leXPs[UBienKnc4SgZZQhe3WkLVexJJdqfGhibXnubY1idCClZXzsJIRm[XSqwrC= M4H5c|I2ODB7Mkm1
SU-DHL-10 NYf3V|RwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXuNVczNzJ3MD:1NFAhdk1? MVyyM|ch\A>? NFnTWm9FVVOR M2X3dolv\HWlZYOgZ4VtdC2leXPs[UBienKnc4SgZZQhe3WkLVexJJdqfGhibXnubY1idCClZXzsJIRm[XSqwrC= Mm[4NlUxODl{OUW=
OCI-Ly3 NH;0[FRCeG:ydH;zbZMhSXO|YYm= M{fHOFE4Oi9{NUCgcm0> NGfNW3o4\A>? M4rNNmROW09? MnrGbY5kemWjc3XzJINie3Cjc3WtN{84KGGldHn2bZR6yqC|aXfubYZq[2GwdHz5 Mn;4NlUxODl{OUW=
OCI-Ly8 M1v1W2Fxd3C2b4Ppd{BCe3OjeR?= MYGxO|IwOjVyIH7N M3jnbldl M3LCWWROW09? M{nPdYlv[3KnYYPld{Bk[XOyYYPlMVMwPyCjY4Tpeol1gcLic3nncolncWOjboTsfS=> NHfDN5ozPTByOUK5OS=>
SU-DHL-4 M2C1fmFxd3C2b4Ppd{BCe3OjeR?= MnLTNVczNzJ3MDDuUS=> M{X1bFdl M3fGWGROW09? M3PXO4lv[3KnYYPld{Bk[XOyYYPlMVMwPyCjY4Tpeol1gcLic3nncolncWOjboTsfS=> MUSyOVAxQTJ7NR?=
SU-DHL-10 MWHBdI9xfG:|aYOgRZN{[Xl? MYexO|IwOjVyIH7N MojQO4Q> M4nSTGROW09? MkDwbY5kemWjc3XzJINie3Cjc3WtN{84KGGldHn2bZR6yqC|aXfubYZq[2GwdHz5 NGXublgzPTByOUK5OS=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pDNA-PKcs / γH2AX / Ub-γH2AX / p-c-Jun S63 / Bax; 

PubMed: 26119999     


The comparative effects of the active (+)-JQ1 vs. the inactive (−)-JQ1 on the cellular responsiveness to radiation and the cell fate decision. 

c-Myc; 

PubMed: 23792448     


PEL and non-PEL (Namalwa) cells treated with indicated doses of (+)-JQ1 for 48 hours were lysed and analyzed by Western blot for the expression of c-Myc. GAPDH was used as a loading control. 

p27; 

PubMed: 23792448     


Western blot analysis showing induction of p27 upon 48 hours of treatment with (+)-JQ1. GAPDH serves as a loading control.

26119999 23792448
Growth inhibition assay
Cell viability; 

PubMed: 23792448     


PEL cell lines (BC1, BC3, BCBL1 and JSC1) were treated with indicated doses of (-)-JQ1 or (+)-JQ1 for 5 days and cell viability measured using an MTS assay (refer to Materials and methods). Namalwa, a Burkitt's lymphoma cell line, was used as a control. 

23792448
Immunofluorescence
GM130; 

PubMed: 29074567     


Representative images of A549cells treated with single compounds or in combination (as indicated) are shown. 

MHC / EdU; 

PubMed: 28733670     


C2C12 cultures were switched to DM and treated with 1 µM (+)-JQ1, (−)-JQ1, DMSO or untreated (DM only). Cells were fixed at 12, 24, 48 and 72 hours after treatment and pulsed with EdU 2 hours before fixation. EdU positive nuclei were stained with Alexa Fluor 555 and cultures immunostained for MHC.

29074567 28733670
体内研究 (+)-JQ1(50 mg/kg)处理携带NMC 797移植瘤的小鼠,抑制肿瘤生长。(+)-JQ1(50 mg/kg) 抹消掉携带NMC 797移植瘤的小鼠的NUT核斑点, 与竞争性结合到核染色质相一致。(+)-JQ1(50 mg/kg)处理NMC 797移植瘤, 显著诱导(31级)角蛋白表达。(+)-JQ1(50 mg/kg)处理携带NMC移植瘤的小鼠模型,促进分化,肿瘤衰退,延长寿命。[1](+)-JQ1(50 mg/kg)处理静脉注射MM.1S-luc+细胞,携带原位移植瘤的SCID米色小鼠,与对照组动物相比,显著延长小鼠的总生存期。[2](+)-JQ1(50 mg/kg,腹腔注射)处理携带Raji移植瘤的小鼠,显著提高小鼠寿命。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

+ 展开
  • Cell lines: MC 11060细胞
  • Concentrations: ~500 nM
  • Incubation Time: 48小时
  • Method:

    细胞按每孔500个接种在白色, 384孔微孔板,孔中含总体为50 μL的培养基。797, TT和TE10细胞生长在含1% Penicillin/Streptomycin 和 10% FBS的DMEM培养基上。Per403细胞生长在含1 % Penicillin/Streptomycin和20% FBS的DMEM 培养基上。病患衍生的NMC 11060细胞生长在含10% FBS 和1% Penicillin/Streptomycin的RPMI培养基上。通过自动化针转移机将(+)-JQ1置于微量检测板上。使细胞裂解,通过增殖试验测评孔中的总ATP含量。根据剂量,重复测量分析,通过逻辑回归(GraphPad Prism)计算IC50。


    (Only for Reference)
动物实验:

[1]

+ 展开
  • Animal Models: 携带NMC 797移植瘤的小鼠
  • Formulation: 5% DMSO溶于5% 葡萄糖
  • Dosages: 50 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 91 mg/mL warmed (199.12 mM)
Ethanol 91 mg/mL (199.12 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 456.99
化学式

C23H25ClN4O2S

CAS号 1268524-70-4
储存条件 粉状
溶于溶剂
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How can I reconstitute the compound for in vivo injection?

  • 回答:

    JQ1 does not dissolve in water/PBS. The vehicle we recommend is 2% DMSO+30% PEG 300+5% Tween 80+ddH2O. The compound can be dissolved in the vehicle at 5mg/ml and you can use it for IV injection.

Epigenetic Reader Domain Signaling Pathway Map

Epigenetic Reader Domain Inhibitors with Unique Features

Tags: 购买(+)-JQ1 | (+)-JQ1供应商 | 采购(+)-JQ1 | (+)-JQ1价格 | (+)-JQ1生产 | 订购(+)-JQ1 | (+)-JQ1代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID