MPTP hydrochloride

For research use only. Not for use in humans.

目录号:S4732

MPTP hydrochloride Chemical Structure

CAS No. 23007-85-4

MPTP HCl是一种多巴胺能的神经毒素,能在帕金森动物模型中选择性破坏多巴胺能神经元。MPTP hydrochloride可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 810.81 现货
RMB 794.79 现货
RMB 2433.3 现货
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客户使用Selleck生产的MPTP hydrochloride发表文献3篇:

产品安全说明书

Dopamine Receptor抑制剂选择性比较

生物活性

产品描述 MPTP HCl是一种多巴胺能的神经毒素,能在帕金森动物模型中选择性破坏多巴胺能神经元。MPTP hydrochloride可诱导凋亡。
体外研究

当N2AB-1和神经胶质瘤细胞暴露于各个浓度下的MPTP时,它们的形态没有发生改变,同时,C6胶质细胞的增殖也没有受到影响[3]。MPTP促进人类成神经细胞瘤M17细胞的凋亡和Tau磷酸化,MPTP显著地提高Tau蛋白在Ser262位的磷酸化水平。MPTP引起人类成神经细胞瘤M17细胞中胞内α-synuclein水平的提升。MPTP通过激活PKA和GSK3β促进脑内Tau的磷酸化[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
insect cells MUjGeY5kfGmxbjDhd5NigQ>? NXjNVnJ2OTJyIH3pcpM> MljLTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCPQV;BJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgZZN{\XO|ZXSgZZMhd3irZHH0bY9vKG:oIHv5cpVz[W2rbnWgd5Vje3S{YYTlJIF1KDVyIIXNJI1m[XO3cnXkJIFnfGW{IHHk[Il1cW:wYXyg[Y57gW2nIHHk[IVlKGGodHXyJFEzOCCvaX7zJIlv[3WkYYTpc44> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5OESxNEc,OjJyN{i0NVA9N2F-
insect cells MmDmSpVv[3Srb36gZZN{[Xl? NYn4TmxRQTBibXnudy=> MUTJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KE2DT1Gg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDhd5Nme3OnZDDhd{BwgGmmYYTpc44hd2Zia4nueZJidWmwZTDzeYJ{fHKjdHWgZZQhPTBidV2gcYVie3W{ZXSgZYZ1\XJiYXTkbZRqd26jbDDzeYJ{fHKjdHWgZYRl\WRiYX\0[ZIhQTBibXnud{BqdmO3YnH0bY9v MmK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{i0NVAoRjJ{MEe4OFExRC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
TH / Actin; 

PubMed: 23391753     


Both CD+/+ and CD+/− mice exhibit MPTP-induced decrease in TH in the striatum. (A) Western blot analyses of TH protein levels in striatal protein extracts after sham or MPTP treatment.

pCaMKIIβ / pCaMKIIα / CaMKIIβ / CaMKIIα / β-Actin; 

PubMed: 31427934     


(E) Effects of SEL, RAS, and PRX on protein expression of CaMKIIα and CaMKIIβ, and their phosphorylation at threonine-286 in the frontal cortex. CaMKIIα (50 kDa), CaMKIIβ (60 kDa), and β-actin (42 kDa).

TH / p-α-Syn / α-Syn / β-actin / CDK5 / LC3-I / LC3-II / p62 / NLRP3 / ASC / Casp1 p20 / Procasp1; 

PubMed: 33717653     


The injection of miR-188-3p-enriched exosomes suppressed the levels of CDK5 and NLRP3 in MPTP-induced PD mice. (A) Western blot results of TH, α-syn, and p-α-syn in sham, MPTP, MPTP + normal exosomes, and MPTP + miR-188-3p-enriched exosome groups in mice SNpc.

p-ERK1/2 / ERK1/2 / GAPDH; 

PubMed: 32927363     


SSTR1 Disrupts the Activation of ERK1/2 Axis in PD In Vitro and In Vivo (A) The protein levels of ERK1/2 and extent of ERK1/2 phosphorylation in response to the treatment with MPTP, MPTP + LV-NC, and MPTP + LV-SSTR1 as determined by western blot analysis; ∗p < 0.05 versus control mice; #p < 0.05 versus MPTP + LV-NC mice. (C) The activation of ERK1/2 in cells treated with oe-NC, oe-SSTR1, si-NC, and si-SSTR1 as determined by western blot analysis; ∗p < 0.05 versus oe-NC cells; #p < 0.05 versus si-NC cells.

GDH2 / GDH1 / GFAP / GAPDH; 

PubMed: 33093440     


c. Expression levels of GDH2, GDH1, and GFAP in the SN of AAV-GFP, MPTP + AAV-GFP, MPTP + AAV-GLUD2, and MPTP + AAV-GLUD2 T1492G groups were determined by western blotting. The bottom band (~55 KD) in the GDH2 blot is non-specific. n = 6.

23391753 31427934 33717653 32927363 33093440
IHC
tyrosine hydroxylase (TH); 

PubMed: 31427934     


(C) Representative photomicrographs of tyrosine hydroxylase (TH) immunohistochemistry in the striatum and substantia nigra of MPTP- or saline-treated mice (upper). Administration of MPTP significantly induced a reduction in TH-positive striatal nerve terminals (lower left) and TH-positive nigral cells (lower right).

tyrosine hydroxylase (TH); 

PubMed: 33717653     


miR-188-3p-enriched exosomes alleviated substantia nigra damage in MPTP-induced mice models  (C) Nissl staining results of mice in sham, MPTP, MPTP + normal exosomes, and MPTP + miR-188-3p-enriched exosome groups. (E) Immunohistochemistry results of TH in sham, MPTP, MPTP + normal exosomes, and MPTP + miR-188-3p-enriched exosome groups.

SNpc; 

PubMed: 33093440     


a. Immunohistochemical staining of TH-positive cells in the SNpc in AAV-GFP, MPTP + AAV-GFP, MPTP + AAV-GLUD2, and MPTP + AAV-GLUD2 T1492G groups (scale bars, 100 µm). The ellipses in the middle column of (a) show the boundaries of the SNpc, and the middle-column boxes denote the areas that are expanded in the right-hand columns. n = 8.

tyrosine hydroxylase (TH); 

PubMed: 11724929     


Dopamine neurons and processes were identified by TH immunostaining of representative midbrain sections 7 days after MPTP treatment. (a) dH2O. (b) MPTP treated.

Substantia nigra; 

PubMed: 17336077     


Alpha-synuclein-immunostaining was performed 7 days after the end of the 28-day-period of chronic continuous MPTP infusion. (A) SNpc of wild-type as well as parkin knockout mice shows significant accumulation of alpha synuclein induced by MPTP treatment. No significant differences are observed between wild-type and parkin knockout mice receiving same treatment (saline or MPTP) (Scale bar= 200μm). (B) High magnification image shows no difference between wildtype and parkin knockout mice following MPTP (Scale bar= 10 μm).

31427934 33717653 33093440 11724929 17336077
Immunofluorescence
tyrosine hydroxylase (TH) / miR-188-3p; 

PubMed: 33717653     


(G) Immunofluorescence results of miR-188-3p and TH in sham, MPTP, MPTP + normal exosomes, and MPTP + miR-188-3p-enriched exosome groups.

α-Syn; 

PubMed: 33717653     


(I) Immunofluorescence results of α-syn in sham, MPTP, MPTP + normal exosomes, and MPTP + miR-188-3p-enriched exosome groups.

tyrosine hydroxylase (TH) / Tunel; 

PubMed: 33717653     


(D) The apoptosis of dopaminergic neurons of the substantia nigra pars compacta in MPTP mice as determined by TUNEL and immunofluorescence assay.

tyrosine hydroxylase (TH) / Tunel; 

PubMed: 33717653     


(E) The apoptosis of dopaminergic neurons of the substantia nigra pars compacta in mice treated with MPTP, MPTP + LV-NC, and MPTP + LV-SSTR1 as assessed by TUNEL and immunofluorescence assay.

tyrosine hydroxylase (TH); 

PubMed: 33093440     


b. Immunofluorescent staining of GFP and TH in the SNpc of AAV-GFP, MPTP + AAV-GFP, MPTP + AAV-GLUD2, and MPTP + AAV-GLUD2 T1492G groups (scale bars, 40 µm).

GFAP; 

PubMed: 33093440     


d. Immunofluorescent staining of GFP and GFAP in the SNpc of AAV-GFP, MPTP + AAV-GFP, MPTP + AAV-GLUD2, and MPTP + AAV-GLUD2 T1492G groups (scale bars, upper, 40 µm; lower, 10 µm). Results are expressed as the mean ± SEM.

33717653 33093440
体内研究 在MPTP处理的小鼠的黑质致密部中,酪氨酸羟化酶阳性的神经元数量减少。在小鼠中脑,MPTP减少1型硫氧还蛋白还原酶的表达和活性、在黑质致密部减少1型硫氧还蛋白还原酶阳性的细胞。MPTP可在人体中及非人类灵长类动物中引起神经化学、行为、组织病理学改变,类似于帕金森患者的症状。相对于灵长类来说,啮齿类动物对MPTP没有那么敏感。MPTP可通过多种途径施用,如填喂法、定位注射法,但最常见、能重复的途径是全身用药,包括皮下、静脉、腹腔、肌内注射。MPTP是一种亲脂性的原毒素,可透过血脑屏障。一定进入脑内,MPTP通过单胺氧化酶B转化为1-methyl-4-phenylpyridine[1]。MPTP在人类、猴和小鼠的黑质纹状体系统中,被证实对多巴胺能神经元具有毒性,持续消耗多巴胺并在纹状体产生其代谢产物[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[3]

- 合并
  • Cell lines: N2AB-1神经细胞系和C6大鼠胶质瘤细胞系
  • Concentrations: 47.7, 4.77, 0.477 μM
  • Incubation Time: 1, 2, 3 天
  • Method:

    将N2AB-1和C6大鼠胶质瘤细胞以50,000个细胞/孔的密度铺于24孔板,在培养基中培养24小时后,将培养基移除,加入新鲜的含不同浓度MPTP或MPP+的培养基。每天收集一批细胞,进行胰蛋白酶化后进行计数,持续3天。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: C57BL/6小鼠
  • Dosages: 20 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 Water 41 mg/mL (195.49 mM)
DMSO 20 mg/mL (95.36 mM)
Ethanol '41 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 209.72
化学式

C12H15N·HCl

CAS号 23007-85-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID