PF-562271

For research use only. Not for use in humans.

目录号:S2890

PF-562271 Chemical Structure

CAS No. 717907-75-0

PF-562271是一种有效的,ATP竞争性的,可逆的FAK抑制剂,无细胞试验中IC50为1.5 nM,作用于FAK比作用于Pyk2选择性高10倍左右,比作用于其他蛋白激酶(除了一些CDKs)选择性高100倍以上。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2862.96 现货
RMB 1392.84 现货
RMB 2194.82 现货
RMB 5469.68 现货
RMB 7967.47 现货
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客户使用Selleck生产的PF-562271发表文献80篇:

产品安全说明书

FAK抑制剂选择性比较

生物活性

产品描述 PF-562271是一种有效的,ATP竞争性的,可逆的FAK抑制剂,无细胞试验中IC50为1.5 nM,作用于FAK比作用于Pyk2选择性高10倍左右,比作用于其他蛋白激酶(除了一些CDKs)选择性高100倍以上。
靶点
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
体外研究

PF-562271会结合到ATP与FAK结合的部位,在激酶铰链区形成抑制剂与主链原子之间的两种氢键。PF-562271在测量FAK磷酸化可诱导的基于细胞的分析中效果很好,IC50为5 nM。PF-562271 (3.3 μM)导致PC3-M细胞周期停滞在G1期[1]。在鸡胚绒毛尿囊膜实验中PF-562271 (1nM)阻断bFGF刺激的血管生成。 PF-262271 强有效的阻断血管生长但对血管渗漏没有明显影响[2]。PF-562271 (250 nM)可以完全抑制整体A431 细胞侵入胶原蛋白中[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 M1;ZTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTBwMke2OkDPxE1? MYTTRW5ITVJ?
SW982 M3nFRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HBfmlEPTB;MD6zNlgzKM7:TR?= MYLTRW5ITVJ?
KM12 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTBwM{i1OVch|ryP Ml\nV2FPT0WU
COLO-205 NFfTTI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jxS2lEPTB;MD60PFY2QCEQvF2= NECw[YxUSU6JRWK=
COLO-829 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwN{WxO|Yh|ryP NI\VTGxUSU6JRWK=
MG-63 MoqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\Ufo5kUUN3ME2wMlgxPjN5IN88US=> MoH6V2FPT0WU
IGROV-1 M3LsW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M332SWlEPTB;MD64NVA{QCEQvF2= MoT6V2FPT0WU
NCI-H650 M{j2b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2n5emlEPTB;MD64N|E2PCEQvF2= NEeybHNUSU6JRWK=
RT-112 NGOzNJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTGOFMzUUN3ME2wMlk5PDZizszN M1rIXnNCVkeHUh?=
BCPAP NIS2ZXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPrNpQ{UUN3ME2xMlAyOjh6IN88US=> MkPmV2FPT0WU
ALL-PO NHzH[pRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzP[25xUUN3ME2xMlAyPTh2IN88US=> NYL3[G5yW0GQR1XS
KYSE-270 MlHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTFwMES3NVQh|ryP M{T4[HNCVkeHUh?=
8305C MoTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\We2ppUUN3ME2xMlA6QTB2IN88US=> M1L4NXNCVkeHUh?=
NCI-H810 MlviS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13nVWlEPTB;MT6xNFc4PiEQvF2= MXPTRW5ITVJ?
CAL-33 NVrVU3lTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4P1eGlEPTB;MT6xNlk{QCEQvF2= NVGzSpR1W0GQR1XS
AN3-CA NXXTRWc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFwMkG4Olch|ryP M1KySXNCVkeHUh?=
NKM-1 Mn3BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4OxdWlEPTB;MT6yO|UxPiEQvF2= M{jkO3NCVkeHUh?=
BPH-1 Mnj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITxVWVKSzVyPUGuNlg4PjZizszN NUiwZppsW0GQR1XS
MES-SA M4rES2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3MTWM2OD1zLkOwOlgzKM7:TR?= MXTTRW5ITVJ?
CAL-62 NHfaNpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLrW|hKSzVyPUGuN|E6ODlizszN NUXaepd2W0GQR1XS
Detroit562 NULoZph1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXqOIhKSzVyPUGuN|UzOjJizszN MVjTRW5ITVJ?
KYSE-150 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1e5dmlEPTB;MT6zOVI{PiEQvF2= Ml25V2FPT0WU
SK-UT-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTFwNES2OFch|ryP M{TUOXNCVkeHUh?=
HUTU-80 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXuTWM2OD1zLkS0PFg3KM7:TR?= NH;RZ2xUSU6JRWK=
SIG-M5 NFm2XnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWr6SoM6UUN3ME2xMlQ5Ozh5IN88US=> MXHTRW5ITVJ?
AGS MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvITWM2OD1zLkWyNVI1KM7:TR?= NYTGdJlPW0GQR1XS
ST486 NEL4[XZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTFwNUOyO|gh|ryP M1PHbHNCVkeHUh?=
HSC-2 NWT5[ZZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DjPWlEPTB;MT61N|k2KM7:TR?= NF7vVIdUSU6JRWK=
LCLC-103H MlnRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke3TWM2OD1zLkW1PFE5KM7:TR?= M4O0VnNCVkeHUh?=
BC-1 NEjycmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jZW2lEPTB;MT62NVY3PCEQvF2= NWLoR4c2W0GQR1XS
CGTH-W-1 NIXIVFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTFwNkG2O|kh|ryP M2XKNXNCVkeHUh?=
MZ1-PC MmHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmKyTWM2OD1zLk[yN|EzKM7:TR?= M1LBenNCVkeHUh?=
SW1710 M1PubWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MluwTWM2OD1zLk[yOlI5KM7:TR?= NFrlSnJUSU6JRWK=
EW-13 MkTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFv4SlNKSzVyPUGuOlM1PjZizszN NYO2T2FUW0GQR1XS
TE-11 NIfkbJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfmd5dKSzVyPUGuOlM2OjJizszN MWrTRW5ITVJ?
BHY NHzzdW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTFwNkW2OlMh|ryP M1;mfXNCVkeHUh?=
ML-2 NI[1WJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTUTWUzUUN3ME2xMlY3QDB{IN88US=> MXLTRW5ITVJ?
MEL-JUSO M2noUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTFwN{K4PVEh|ryP M4DndHNCVkeHUh?=
U251 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XjRmlEPTB;MT63OFA{OSEQvF2= NHLOToRUSU6JRWK=
MHH-ES-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jFXGlEPTB;MT63PVU3QCEQvF2= MVXTRW5ITVJ?
CAL-85-1 NWe5N|ZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrGU5psUUN3ME2xMlgxPDlizszN NWX2N4xRW0GQR1XS
D-336MG M3;FVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTFwOEW2NVQh|ryP NXHSSGpmW0GQR1XS
VA-ES-BJ NYXKVGtvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTFwOUCzNVUh|ryP MVXTRW5ITVJ?
MDA-MB-468 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXSWIJxUUN3ME2xMlkyOjd{IN88US=> M1G4[nNCVkeHUh?=
MEL-HO NHflNmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHn6fnpKSzVyPUGuPVc6QTNizszN M{fwdnNCVkeHUh?=
SW954 NF\n[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M365SGlEPTB;Mj6wNFE2OSEQvF2= M4nPbnNCVkeHUh?=
LB831-BLC Ml;WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3S4V2lEPTB;Mj6wNFk5QCEQvF2= M1TnO3NCVkeHUh?=
EW-16 Mm[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTJwMEO5PFMh|ryP NVu3VWF7W0GQR1XS
NCI-H460 M3LJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDyUJFCUUN3ME2yMlA1QDN7IN88US=> NUTlbnJDW0GQR1XS
CHP-212 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLBTWM2OD1{LkC5OVM6KM7:TR?= MWXTRW5ITVJ?
MHH-NB-11 M3;mSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;xeGlEPTB;Mj6xNlgyQCEQvF2= MmPTV2FPT0WU
DU-4475 MkfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHPTWM2OD1{LkG0O|U6KM7:TR?= NY\HeFlKW0GQR1XS
ABC-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTDUWxwUUN3ME2yMlI2OTl4IN88US=> MmTnV2FPT0WU
LOUCY MnPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4Cyb2lEPTB;Mj6yOlEzQCEQvF2= MofxV2FPT0WU
LC-2-ad NV7xenRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTJwMk[5OlYh|ryP NF[zeYtUSU6JRWK=
ETK-1 NUTXeWxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHnS4JsUUN3ME2yMlI5PDZizszN MWDTRW5ITVJ?
KYSE-180 NWHvW5Z{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nGcGlEPTB;Mj6yPFg4PCEQvF2= M4XEUnNCVkeHUh?=
HOP-92 M{nne2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTJwM{C2O|Mh|ryP Ml3IV2FPT0WU
RCC10RGB MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTJwM{K1NFch|ryP M3u0NXNCVkeHUh?=
KASUMI-1 M2f3PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTYcpJKSzVyPUKuN|I5QCEQvF2= NVHQcXFTW0GQR1XS
ACN NVPiSWRuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVf3SmdpUUN3ME2yMlM1PzR3IN88US=> NXnCdWpPW0GQR1XS
BxPC-3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mof4TWM2OD1{LkO1OFM3KM7:TR?= NFTWXnpUSU6JRWK=
MONO-MAC-6 NEPPfmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTlTWM2OD1{LkSxNlQ5KM7:TR?= NIXoboFUSU6JRWK=
CAL-39 NEfrS4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTJwNEG2Nlgh|ryP MVTTRW5ITVJ?
CMK NFvXfotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnKOo55UUN3ME2yMlQyPzl3IN88US=> NWC5e3Z3W0GQR1XS
MFE-296 M2Todmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXITWM2OD1{LkS3O|kzKM7:TR?= MoPyV2FPT0WU
DU-145 MlrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTJwNEmxNVgh|ryP NXzo[XR7W0GQR1XS
MDA-MB-231 MlrES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXqz[IU6UUN3ME2yMlQ6PTd{IN88US=> NIHvVG1USU6JRWK=
EFO-27 NXPGUJFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LPZmlEPTB;Mj61NVg5QCEQvF2= NXTCdHlzW0GQR1XS
SNU-387 Mmn1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPPTWM2OD1{LkWyPFIh|ryP MYfTRW5ITVJ?
KYSE-410 NYPabVRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTXTWM2OD1{LkW2OVE4KM7:TR?= M{jwOHNCVkeHUh?=
NCCIT MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfSW3k6UUN3ME2yMlU4OjRizszN M{jtZnNCVkeHUh?=
LXF-289 M2DXdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTJwNUiyO|Mh|ryP MmTPV2FPT0WU
HMV-II MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTJwNUi5PVch|ryP NW\o[I1WW0GQR1XS
MOLT-4 MnLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTJwNkC3NVIh|ryP NIrYXIpUSU6JRWK=
KOSC-2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXM[FdKSzVyPUKuOlM5PzlizszN M1[4cXNCVkeHUh?=
TUR NXrPRVNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInKWHVKSzVyPUKuOlU{PjlizszN NFzMPGhUSU6JRWK=
NCI-H2087 NF;vTG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NECyNFlKSzVyPUKuO|E3PDZizszN Ml[yV2FPT0WU
SCC-15 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTFTWM2OD1{Lke1NlUzKM7:TR?= MoLQV2FPT0WU
HSC-4 MoXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGj6dmVKSzVyPUKuO|g{QDlizszN MWHTRW5ITVJ?
G-402 NYLPOox4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHz5ZnJKSzVyPUKuPFE1PDdizszN NWroZY5TW0GQR1XS
GP5d NVK5OlI6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XYdmlEPTB;Mj64N|gzPyEQvF2= NX;mem0yW0GQR1XS
IGR-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfINnlKSzVyPUKuPFM6QDdizszN MVrTRW5ITVJ?
SK-PN-DW NXPzfVFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTJwOE[yNFQh|ryP NIDm[ZBUSU6JRWK=
OPM-2 MnzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2qz[GlEPTB;Mj64PVk4QCEQvF2= MV\TRW5ITVJ?
5637 NHG0SldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjwO2JKSzVyPUKuPVI1OzlizszN MkS1V2FPT0WU
NCI-H2030 NGLp[3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnj5TWM2OD1{Lkm4NFI6KM7:TR?= NX7H[5BTW0GQR1XS
SK-LU-1 NIHyXHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTzTWM2OD1{Lkm4N|M1KM7:TR?= MYXTRW5ITVJ?
EW-18 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnDT5l5UUN3ME2zMlA2OzJizszN M1y2enNCVkeHUh?=
786-0 MnfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnFdXhzUUN3ME2zMlA2Pzh2IN88US=> NET0[G9USU6JRWK=
NCI-H1963 NGHDfZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIf3XFRKSzVyPUOuNFg5OjVizszN MmDaV2FPT0WU
A2780 NX3Bb486T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXPTWM2OD1|LkGwN|QyKM7:TR?= NGW2dYNUSU6JRWK=
NCI-H526 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PEUWlEPTB;Mz6xN|k3OSEQvF2= NHS2NJJUSU6JRWK=
BHT-101 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTNwMUW3PVgh|ryP M3TTU3NCVkeHUh?=
RPMI-7951 NFLC[WlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHLXpRKSzVyPUOuNlAyOzdizszN M{e3RnNCVkeHUh?=
HL-60 NYHkTGg3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnn4TWM2OD1|LkKxNVQh|ryP MYrTRW5ITVJ?
CTV-1 NGXjbG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTNwMkWzO|ch|ryP MWLTRW5ITVJ?
IST-SL1 M37BTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnr3TWM2OD1|LkK5OFY2KM7:TR?= NV;hZW1iW0GQR1XS
G-401 Ml3NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTDO3dKSzVyPUOuNlk5ODhizszN M1;wZXNCVkeHUh?=
OVCAR-5 NUXIe3ZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HlVWlEPTB;Mz6zOlM2OiEQvF2= Mnm0V2FPT0WU
EW-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XJN2lEPTB;Mz60NFU1PCEQvF2= NGK3TVNUSU6JRWK=
H4 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2mxe2lEPTB;Mz60O|U3QSEQvF2= NEPOd4xUSU6JRWK=
EM-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLwTWM2OD1|LkS4Olc1KM7:TR?= M4C3[XNCVkeHUh?=
RS4-11 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUK0R2NxUUN3ME2zMlQ5PzF3IN88US=> NHGyZWZUSU6JRWK=
NOMO-1 MoSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkK0TWM2OD1|LkS5NlA1KM7:TR?= M3;OW3NCVkeHUh?=
HCT-116 M1fkS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTNwNUC3NVEh|ryP NFXJSXBUSU6JRWK=
CAL-120 M3LyT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmj0TWM2OD1|LkW3N|Uh|ryP NIracmVUSU6JRWK=
CAL-51 MnW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTNwNUe2NVIh|ryP MWfTRW5ITVJ?
RVH-421 M1PiSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moj6TWM2OD1|Lk[wN|EyKM7:TR?= MXXTRW5ITVJ?
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U-87-MG M{fJeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjUOFB6UUN3ME2yOk42OzB3IN88US=> M{D3VnNCVkeHUh?=
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HDLM-2 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTJ6LkK0OVkh|ryP NWW4WGJXW0GQR1XS
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CAL-54 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoexTWM2OD1{OT62NFQ3KM7:TR?= M1LST3NCVkeHUh?=
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NCI-H596 M3Pz[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjDboFKSzVyPUOxMlA3OjFizszN M1jafXNCVkeHUh?=
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SW620 MnLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTNzLk[yPFMh|ryP M2\SXHNCVkeHUh?=
KM-H2 NFfE[GtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4SwcGlEPTB;M{GuO|MxPiEQvF2= M1;6XHNCVkeHUh?=
DJM-1 NG\0PYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1OwXmlEPTB;M{KuNVM5PiEQvF2= MlG0V2FPT0WU
SW48 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLETWM2OD1|Mj6zPFI2KM7:TR?= M{i0WnNCVkeHUh?=
NCI-H1793 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPrSJFCUUN3ME2zNk43OTZ|IN88US=> M1HZcnNCVkeHUh?=
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NCI-H441 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjSZlJKSzVyPUOzMlIzOzVizszN MWrTRW5ITVJ?
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IA-LM NFO1d5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfJOXlEUUN3ME2zN{43PzR4IN88US=> M4P5cnNCVkeHUh?=
NCI-H1417 NUCzUYpHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV3TOndMUUN3ME2zN{46PTh3IN88US=> NXXVW|M{W0GQR1XS
HCC2218 MnTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjCXnA4UUN3ME2zOU4zOTh3IN88US=> NX[4dG41W0GQR1XS
HC-1 M{jPOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPuTWM2OD1|NT60NFI1KM7:TR?= NHvHTm1USU6JRWK=
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EFE-184 NFTXOZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrpUoNkUUN3ME2zOU43QDJ7IN88US=> MWfTRW5ITVJ?
NCI-H1355 NXPCV|ViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXtUpZoUUN3ME2zOU44PzF6IN88US=> MU\TRW5ITVJ?
BV-173 NHLkOFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfKTWM2OD1|NT64NlY1KM7:TR?= MYPTRW5ITVJ?
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KYSE-140 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\aOG8xUUN3ME2zOk44Ojl6IN88US=> MorwV2FPT0WU
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HCC1937 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPYTnVFUUN3ME2zO{4xOTV2IN88US=> M3fwZ3NCVkeHUh?=
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RCM-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTN5LkWzOVgh|ryP MWnTRW5ITVJ?
A375 M{DKemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHqZW1ZUUN3ME2zO{44PjR5IN88US=> NE\Fe2NUSU6JRWK=
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MDA-MB-453 NVPyNWFyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkftTWM2OD1|OT6wNlE5KM7:TR?= MWfTRW5ITVJ?
Capan-2 NHT1PVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP4O415UUN3ME2zPU4xPTB7IN88US=> MkjFV2FPT0WU
SW756 MmDaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[0SpV7UUN3ME2zPU42ODlzIN88US=> M{DWTnNCVkeHUh?=
KU-19-19 M373eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFywbIVKSzVyPUO5MlYzQDJizszN MlrOV2FPT0WU
NCI-H2170 MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXIeHo{UUN3ME2zPU46QTd5IN88US=> NHm3OYpUSU6JRWK=
COLO-678 MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37FdGlEPTB;NECuOVk2KM7:TR?= M2\kZXNCVkeHUh?=
PF-382 M2\yWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYizOpNIUUN3ME20NE44PDh5IN88US=> MknvV2FPT0WU
OAW-42 NEjZO3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTR{LkK5OFIh|ryP NVvRWldMW0GQR1XS
LB996-RCC MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2n1d2lEPTB;NEKuPFAxQCEQvF2= NWT0S4p5W0GQR1XS
SAS M{iyTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7qTWM2OD12Mz60Nlg5KM7:TR?= NWLDSFJsW0GQR1XS
MRK-nu-1 NF7scGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfiZ2lKSzVyPUS0Mlc1OTRizszN M1:2U3NCVkeHUh?=
EoL-1-cell MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk[4TWM2OD12ND65NVM3KM7:TR?= NVW0fJo6W0GQR1XS
NCI-H2342 MmjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1i0W2lEPTB;NEWuNVEyPyEQvF2= NVr4SVVnW0GQR1XS
NCI-H1703 Mnf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGC4XI9KSzVyPUS2MlA2QTJizszN MWHTRW5ITVJ?
NCI-H187 NF;ufnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELKWXpKSzVyPUS2MlE5PjlizszN NX70[HQ{W0GQR1XS
CP66-MEL NVf3TpNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGf6bVlKSzVyPUS2MlI4QDlizszN NXHPTpdXW0GQR1XS
HCC1569 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;WeItKSzVyPUS2MlU6OTdizszN MV\TRW5ITVJ?
BL-70 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXBN41KSzVyPUS2Mlc4OzdizszN NGjNWHFUSU6JRWK=
Saos-2 NH3aSFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn[zTWM2OD12Nz6zNVI{KM7:TR?= NV2wPFM2W0GQR1XS
LC-1F MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzNTWM2OD12Nz62NVA2KM7:TR?= MVPTRW5ITVJ?
KY821 M4\sOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvFTWM2OD12Nz62OFM1KM7:TR?= Mn\mV2FPT0WU
PSN1 NUfGWms6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHMc285UUN3ME20PE4yPDBzIN88US=> MYHTRW5ITVJ?
RT4 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHXVWNKSzVyPUS4Mlg{PzdizszN NXXLZXpiW0GQR1XS
LS-1034 MlXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTR6Lki5OVQh|ryP NHnYXJVUSU6JRWK=
NCI-H1395 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TKUGlEPTB;NEmuNFQ6OiEQvF2= NHjNclZUSU6JRWK=
EKVX MmjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTR7Lk[3N|Eh|ryP MVzTRW5ITVJ?
IST-MES1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTR7Lki5OFQh|ryP MUHTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-FAK / FAK / p-Talin / Talin ; 

PubMed: 26530902     


Dose response of PF-562271 on the phosphorylation of FAK-Y397 or Talin-S425 in PC3-mm2 cells.

p-Src / Src / p-paxillin / Paxillin / ICAM-1 ; 

PubMed: 29417941     


MDA-MB-231 cells transfected with FAK-specific siRNAs or treated for 12 h with PF-562271 at the indicated concentrations were subjected to immunoblotting with indicated antibodies.

26530902 29417941
Immunofluorescence
p-FAK; 

PubMed: 26530902     


Immunostaining of PC3-mm2 cells showed that phosphorylated FAK is localized at focal adhesions (inset).

26530902
Growth inhibition assay
Cell viability; 

PubMed: 28953264     


MTT assay showed inhibition of cell proliferation and viability in HCT116 (B) and SW480 (D) cell lines treated with resveratrol and/or different concentrations of FAK-Inhibitor (PF-562271: 0.01, 0.1, 1, and 10 µM) cultured in monolayer for 12 h. Data shown are representative of three independent sets of experiments. Values were compared with the control and statistically significant values with p < 0.05 are designated by an asterisk (*) and p < 0.01 are designated by two asterisks (**).

28953264
体内研究 在携带U87MG的小鼠中PF-562271 (< 33 mg/kg 口服)抑制肿瘤中FAK磷酸化并具有时间和剂量依赖特性。PF-562271 (50 mg/kg, 口服,一天两次)对BxPc3异种移植小鼠中肿瘤生长抑制率为86% ,对PC3-M异种移植小鼠中肿瘤生长抑制率为45%。PF-562271 (25mg/kg, 一天两次)处理的携带H125肺部异种移植肿瘤凋亡率提高2倍[1]。 PF-562271 (33 mg/kg,口服)24小时后抑制小鼠中肿瘤细胞的广泛运动。PF-562271 (33 mg/kg, 口服)导致小鼠中E-cadherin动力学的改变,这与减弱的E-cadherin依赖的整体细胞运动有关 [3]。在皮下局部异种移植PC3M-luc-C6的小鼠模型中 PF-562271 (25 mg/kg, 口服,一天两次)导致肿瘤生长率62%的抑制[4]。在胫骨植入MDA-MB-231细胞的大鼠中PF-562271 (5mg/kg, 口服)导致骨钙素和松质骨参数显著而相似的增加同时导致肿瘤生长速度和骨愈合信号的下降[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1]
- 合并
  • Animal Models: 携带BxPc3或 PC3-M异种移植的无胸腺雌性小鼠
  • Dosages: 50 mg/kg
  • Administration: 填喂给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
5 mg/ml

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 507.49
化学式

C21H20F3N7O3S

CAS号 717907-75-0
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

FAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID