PF-562271

目录号:S2890 别名: PF-00562271

仅限科研使用

PF-562271 (PF-00562271)是一种有效的,ATP竞争性的,可逆的FAK抑制剂,无细胞试验中IC50为1.5 nM,作用于FAK比作用于Pyk2选择性高10倍左右,比作用于其他蛋白激酶(除了一些CDKs)选择性高100倍以上。

PF-562271 Chemical Structure

CAS: 717907-75-0

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 2862.96 现货
RMB 1392.84 现货
RMB 5469.68 现货
大包装 有超大折扣
大剂量询单有大折扣!

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的PF-562271发表文献105篇:

产品安全说明书

FAK抑制剂选择性比较

相关FAK产品

生物活性

产品描述 PF-562271 (PF-00562271)是一种有效的,ATP竞争性的,可逆的FAK抑制剂,无细胞试验中IC50为1.5 nM,作用于FAK比作用于Pyk2选择性高10倍左右,比作用于其他蛋白激酶(除了一些CDKs)选择性高100倍以上。
靶点
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
点击更多
1.5 nM 13 nM 30 nM 47 nM 58 nM
体外研究

PF-562271会结合到ATP与FAK结合的部位,在激酶铰链区形成抑制剂与主链原子之间的两种氢键。PF-562271在测量FAK磷酸化可诱导的基于细胞的分析中效果很好,IC50为5 nM。PF-562271 (3.3 μM)导致PC3-M细胞周期停滞在G1期[1]。在鸡胚绒毛尿囊膜实验中PF-562271 (1nM)阻断bFGF刺激的血管生成。 PF-262271 强有效的阻断血管生长但对血管渗漏没有明显影响[2]。PF-562271 (250 nM)可以完全抑制整体A431 细胞侵入胶原蛋白中[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
IST-MES1 MmPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV74eXNlUUN3ME20PU45QTR2IN88US=> NVK0Zm8xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
EKVX M4j2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDSTWM2OD12OT62O|MyKM7:TR?= Mn;lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
NCI-H1395 NHvMWnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTR7LkC0PVIh|ryP MoXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
LS-1034 M1G3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1[zbmlEPTB;NEiuPFk2PCEQvF2= NV;YV5hSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
RT4 NUfhPFNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PlSGlEPTB;NEiuPFM4PyEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
PSN1 M3HOTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLjTngyUUN3ME20PE4yPDBzIN88US=> NFfDfJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODh2NUS2M{c,W0GQR1XSQE9iRg>?
KY821 M2DnT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLKbFBKSzVyPUS3MlY1OzRizszN NV;JOI9bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
LC-1F MnO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTR5Lk[xNFUh|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
Saos-2 M2nteWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXO2WnZ6UUN3ME20O{4{OTJ|IN88US=> NF33U4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODh2NUS2M{c,W0GQR1XSQE9iRg>?
BL-70 NWm5eGVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTaTWM2OD12Nj63O|M4KM7:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
HCC1569 MnzWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NImzXZhKSzVyPUS2MlU6OTdizszN NUHsfmFtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
CP66-MEL MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfqTWM2OD12Nj6yO|g6KM7:TR?= NXzUWYhVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
NCI-H187 NGnpZ41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFz1W4lKSzVyPUS2MlE5PjlizszN M{XhbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
NCI-H1703 MnHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPSTWM2OD12Nj6wOVkzKM7:TR?= NVPacmV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
NCI-H2342 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTR3LkGxNVch|ryP M17YW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
EoL-1-cell MmfaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PQPGlEPTB;NESuPVE{PiEQvF2= MoLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
MRK-nu-1 M13BfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFP4NlNKSzVyPUS0Mlc1OTRizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
SAS M3G4TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXU[3FKSzVyPUSzMlQzQDhizszN M{XmU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
LB996-RCC M3rocGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXkcJVVUUN3ME20Nk45ODB6IN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
OAW-42 M2fSXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mln3TWM2OD12Mj6yPVQzKM7:TR?= NVHBdXQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
PF-382 NWrwfHVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LHOmlEPTB;NECuO|Q5PyEQvF2= NG\ONpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODh2NUS2M{c,W0GQR1XSQE9iRg>?
COLO-678 M1fDcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTRyLkW5OUDPxE1? MkjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
NCI-H2170 NHexO2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTN7Lkm5O|ch|ryP NEjKRXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODh2NUS2M{c,W0GQR1XSQE9iRg>?
KU-19-19 MkC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTN7Lk[yPFIh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
SW756 NFW3V3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\MfZBwUUN3ME2zPU42ODlzIN88US=> NXH1fmI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
Capan-2 NITaWWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3f0PGlEPTB;M{muNFUxQSEQvF2= NXHF[5V6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
MDA-MB-453 M{\lXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13YZWlEPTB;M{muNFIyQCEQvF2= NYLIbolFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
TGBC24TKB Mn\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjkVoRKSzVyPUO4MlAzOzlizszN MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
A375 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fQZmlEPTB;M{euO|Y1PyEQvF2= M3L2SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
RCM-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\mUmVKSzVyPUO3MlU{PThizszN M3K5WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
KARPAS-422 NHrPbYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fMRWlEPTB;M{euOFQ{OSEQvF2= NYTnZYFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
LNCaP-Clone-FGC NXvHR5R[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml6xTWM2OD1|Nz6xPVA{KM7:TR?= M2C5e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
HCC1937 M4\tWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHhUG5KSzVyPUO3MlAyPTRizszN MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
no-11 NULOOIx6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTN4Lki1N|Yh|ryP NV3QfZltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
KYSE-140 Mn62S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2WycWlEPTB;M{[uO|I6QCEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFg1PTR4Lze+V2FPT0WUPD;hQi=>
SW1463 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvSXoNuUUN3ME2zOk43PTZ4IN88US=> M3HJOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
NEC8 M4HOcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVq3NGlxUUN3ME2zOk41QTJ2IN88US=> Mor4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
BV-173 MlT6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTN3LkiyOlQh|ryP NV7uT21DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB6NEW0Ok8oRlODTlfFVlww[T5?
NCI-H1355 M33Id2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPNTWM2OD1|NT63O|E5KM7:TR?= MkPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
EFE-184 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfNVWtKSzVyPUO1MlY5OjlizszN M{TiO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQDR3NE[vK|5USU6JRWK8M4E,
HH NUXGRnhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTN3LkSxOFEh|ryP MkHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA5PDV2Nj:nQnNCVkeHUkyvZV4>
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Saos-2 M3;Zc5FJXFNiYYPzZZk> NGHDXWtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? NYnOUW85RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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SK-N-SH MWfxTHRUKGG|c3H5 NFHjOmpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLWPIJINmdGy| MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Assay
Methods Test Index PMID
Western blot p-FAK / FAK / p-Talin / Talin p-Src / Src / p-paxillin / Paxillin / ICAM-1 26530902 29417941
Immunofluorescence p-FAK 26530902
Growth inhibition assay Cell viability 28953264
体内研究 在携带U87MG的小鼠中PF-562271 (< 33 mg/kg 口服)抑制肿瘤中FAK磷酸化并具有时间和剂量依赖特性。PF-562271 (50 mg/kg, 口服,一天两次)对BxPc3异种移植小鼠中肿瘤生长抑制率为86% ,对PC3-M异种移植小鼠中肿瘤生长抑制率为45%。PF-562271 (25mg/kg, 一天两次)处理的携带H125肺部异种移植肿瘤凋亡率提高2倍[1]。 PF-562271 (33 mg/kg,口服)24小时后抑制小鼠中肿瘤细胞的广泛运动。PF-562271 (33 mg/kg, 口服)导致小鼠中E-cadherin动力学的改变,这与减弱的E-cadherin依赖的整体细胞运动有关 [3]。在皮下局部异种移植PC3M-luc-C6的小鼠模型中 PF-562271 (25 mg/kg, 口服,一天两次)导致肿瘤生长率62%的抑制[4]。在胫骨植入MDA-MB-231细胞的大鼠中PF-562271 (5mg/kg, 口服)导致骨钙素和松质骨参数显著而相似的增加同时导致肿瘤生长速度和骨愈合信号的下降[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1]
  • Animal Models: 携带BxPc3或 PC3-M异种移植的无胸腺雌性小鼠
  • Dosages: 50 mg/kg
  • Administration: 填喂给药

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O

5 mg/ml

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 507.49
化学式

C21H20F3N7O3S

CAS号 717907-75-0
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

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