Piperlongumine

For research use only. Not for use in humans.

目录号:S7551 别名: PPLGM, Piplartine 中文名称:荜茇酰胺

Piperlongumine Chemical Structure

CAS No. 20069-09-4

Piperlongumine (PPLGM, Piplartine),存在于胡椒科植物荜茇中的一种天然生物碱,能够增加reactive oxygen species (ROS)水平,并选择性杀死癌细胞。它还是TrxR1的直接抑制剂,对胃癌具有抑制活性;一种新型的CRM1抑制剂;在人类乳腺癌细胞中可抑制PI3K/Akt/mTOR

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RMB 790.72 现货
RMB 2911.49 现货
RMB 7749.45 现货
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客户使用Selleck生产的Piperlongumine发表文献19篇:

产品安全说明书

ROS抑制剂选择性比较

生物活性

产品描述 Piperlongumine (PPLGM, Piplartine),存在于胡椒科植物荜茇中的一种天然生物碱,能够增加reactive oxygen species (ROS)水平,并选择性杀死癌细胞。它还是TrxR1的直接抑制剂,对胃癌具有抑制活性;一种新型的CRM1抑制剂;在人类乳腺癌细胞中可抑制PI3K/Akt/mTOR
靶点
reactive oxygen species (ROS) [1] TrxR1 [5] CRM1 [6] PI3K/Akt/mTOR [7]
体外研究

Piperlongumine是一种已知的ROS诱导剂,可诱导胰腺癌细胞的细胞死亡[2]。作为一种血栓素A(2)受体拮抗剂,Piperlongumine抑制血小板聚集。[2] Piperlongumine也能够通过抑制Akt/mTOR信号通路促进细胞自噬,并介导肿瘤细胞死亡。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ZR75-30 Mlq3R5l1d3SxeHnjbZR6KGG|c3H5 MVG3NkBpenN? NWnhVZhpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hYlJ5NT2zNEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTVwOEdOwG0> MoXrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7M{exPFYoRjJ2OUO3NVg3RC:jPh?=
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MDA-MB-231 MYTDfZRwfG:6aXPpeJkh[XO|YYm= MYG3NkBpenN? M4\rcWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD16LkS2{txO NVuxS49YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5N|cyQDZpPkK0PVM4OTh4PD;hQi=>
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MRC5 NX\He2RJS3m2b4TvfIlkcXS7IHHzd4F6 M2jsbFczKGi{cx?= NF;J[4FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|UvODUQvF2= Ml;PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7M{exPFYoRjJ2OUO3NVg3RC:jPh?=
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RAW264.7 NFHsN2VHfW6ldHnvckBie3OjeR?= MYCxNEB2VQ>? M1vsZVAvPSCqcoO= NGjaflFKdmirYnn0bY9vKG:oIF3BVGswVk[tYYDwZWIhcW5iSVPSJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBNWFNvaX7keYNm\CCLTE[gdJJw\HWldHnvckBifCBzMDD1UUBxemW2cnXheIVlKG[xcjCwMlUhcHK|IH\vcIxwf2WmIHL5JGxRWyClaHHscIVv\2ViYX7kJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSC{ZXzheIl3\SC2bzDjc451em:u NXzPOFB1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PFAxQDhpPkOwO|gxODh6PD;hQi=>
BHK21 NXvLepRnS3m2b4TvfIlkcXS7IHHzd4F6 NX7qXYxkPDhiaILz NWK4T483S3m2b4TvfIlkcXS7IHHnZYlve3RiQljLNlEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEOFNUC9OFAvOTUQvF2= Mo[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyMEm5NFgoRjNzMEC5PVA5RC:jPh?=
SH-SY5Y MUjDfZRwfG:6aXPpeJkh[XO|YYm= NWOzTndnPzJiaILz NVnFWYhIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0hvU2m1XUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUWuPVTPxE1? MoLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2MEC3NFgoRjNzNECwO|A5RC:jPh?=
U87 NY\QVW1xS3m2b4TvfIlkcXS7IHHzd4F6 MWi3NkBpenN? Mnu0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVg4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9O{4yQM7:TR?= MlnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2MEC3NFgoRjNzNECwO|A5RC:jPh?=
SH-SY5Y NFTwXnFHfW6ldHnvckBie3OjeR?= M1H6WFE2KG2rboO= NWnQe5EzUW6qaXLpeIlwdiCxZjDUdphTKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgeZNqdmdiRGTORkBieyC|dXLzeJJifGVicILlbY5kfWKjdHXkJIZweiBzNTDtbY5{KG[xbHzve4VlKGK7IIP1ZpN1emG2ZTDh[IRqfGmxbjDhcoQhdWWjc4Xy[YQh\m:{IEKwJI1qdnNiYomgSYxtdWGwJ4OgcYV1cG:mLDDJR|UxRTR3Lki4{txO MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTRyMEewPEc,OzF2MEC3NFg9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT / p-S6 / S6 ; 

PubMed: 30906213     


HCC827 (left), H1975 (middle) and H23 cells were treated with piperlongumine for 24 h, western blot was conducted to detect the target proteins as indicated.

Hexokinase 1 / Hexokinase 2 ; 

PubMed: 30906213     


Piperlongumine regulates glycolysis in NSCLC cells. NSCLC cells, including H23 (A), HCC827 (B) and H1975 (C) were treated with different concentrations of piperlongumine for 24 h. Western blotting was performed to detect HK2 expression (left). The levels of glucose consumption (middle) and lactate production (right) were examined in these cells. Columns, mean of the number of colonies as determined from three independent experiments; bars, standard deviation; asterisk, significant (*p<0.05, **p<0.01, ***p<0.001) suppression of HK2 expression or glycolysis by piperlongumine compared with DMSO treated group. PL, Piperlongumine.

p-STAT3 / STAT3 / p-JAK2 / JAK2 / c-myc ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for different amounts of time, and STAT3, p-STAT3, p-JAK2, JAK2 and c-myc levels were then measured.

Survivin / p21 / p27 ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for different amounts of time. Survivin, p21, and p27 levels were then measured.

Bax / Bcl-2 ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for 12 or 24 h and Bcl-2 and Bax levels were measured. Quantitative analysis was performed using Image J software, with normalization to GAPDH expression.

CDK2 / Cyclin E / Cyclin A ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for 12, 24, or 48 h; cyclins and CDK2 levels were then measured

30906213 27634873
Growth inhibition assay
Cell viability; 

PubMed: 25193861     


Piperlongumine induces death in HNC cells but not normal cells. Cytotoxicity was assessed by MTT assay 

25193861
体内研究 Piperlongumine (50 mg/kg i.p.)引起体内肿瘤细胞的生长抑制,而不改变生物化学,血液学和组织病理学参数。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[3]

- 合并
  • Cell lines: MCF-7 和 786-O 细胞
  • Concentrations: ~10 μM
  • Incubation Time: 48小时
  • Method:

    MCF-7和786-O细胞与不同浓度的PL培养48小时。细胞增殖通过CellTiter Blue测试分析。有效剂量(ED)使用XLift,Microsoft Excel加载项计算。


    (Only for Reference)
动物实验:

[4]

- 合并
  • Animal Models: 移植肉瘤 180 肿瘤的小鼠
  • Dosages: ~50 mg/kg
  • Administration: i.p.
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 16 mg/mL warmed (50.41 mM)
Ethanol 6 mg/mL warmed (18.9 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 317.34
化学式

C17H19NO5

CAS号 20069-09-4
储存条件 粉状
溶于溶剂
别名 PPLGM, Piplartine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID