Tocilizumab (anti-IL-6R)

目录号:A2012 别名: MRA 中文名称:托珠单抗

For research use only.
Not for use in humans.

Tocilizumab (anti-human IL-6R)是人源单克隆单体,可与interleukin-6 receptor结合发挥抑制作用。MW: 148 KD。

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质量管理及产品安全说明书

IL Receptor抑制剂选择性比较

生物活性

产品描述 Tocilizumab (anti-human IL-6R)是人源单克隆单体,可与interleukin-6 receptor结合发挥抑制作用。MW: 148 KD。
靶点
IL-6R [1]
()
体外研究

Tocilizumab (anti-IL-6R)抑制IL-6与其受体结合,与其竞争性地结合可溶性和膜结合的IL-6受体,因而减少细胞因子促炎性活性。Tocilizumab的抑制活性与膜结合的IL-6表达无关[1]。Tocilizumab通过诱导凋亡,具有抗癌效力。它通过抑制JAK-STAT3通路,对神经胶质瘤细胞具有抗增殖活性。Tocilizumab对NSCLC细胞具有显著的生长抑制作用,其中,A549细胞的增殖显著降低,降低了约40%。Tocilizumab不改变ERK1/2、STAT3、NFκB和磷酸化ERK1/2、STAT3的蛋白水平,在NSCLC细胞中,它增加了磷酸化NFκB的表达[2]。Tocilizumab显著地移植IL-8和MMP-9的表达[3]

体内研究 一系列临床研究证明,通过tocilizumab抑制IL-6信号在治疗风湿性关节炎、幼年特发性关节炎、巨淋巴结增生症、克罗恩病中十分有效。在这些疾病中,tocilizumab可减缓炎症临床表现,使急性期蛋白水平回归正常。Tocilizumab不管是单药给药还是组合给药(如与methotrexate),耐受性良好。Tocilizumab可显著地减少肿瘤中入侵毛细管的数量。除此之外,tocilizumab肿瘤细胞有丝分裂的速度和凋亡没有作用[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: To validate that IL-6 signaling is essential for TAM (tumor-associated macrophages)-enhanced CSC (cancer stem cells) function
    Cells: HepG2 cell lines, TAMs isolated from HCCs (hepatocellular carcinoma)
    Concentrations: 5 μg/ml
    Incubation Time: 72 h
    Method: For co-culture experiments, 12-well Transwell chambers were used with 2×105 HepG2 or Hep3B cells seeded in the lower chamber one day before co-culture and either 5×105 or 1×106 TAMs added in the upper chamber. Tocilizumab or human IgG antibody were added in indicated experiments to selected wells. After 24 to 72 hours, HCC cells and CD14+ TAM cells were collected separately for analysis.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253315/

    Objective: Effect of tocilizumab on the growth of Lung Cancer Cells
    Cells: human-derived HARA-B cells
    Concentrations: 1, 10, 100 ng/ml
    Incubation Time: 48 h
    Method: HARA-B cells were cultured for 24 h in DMEM, then in serum-free DMEM for 24 h and then for 48 h in HACM (serum-free), with or without tocilizumab (1, 10, and 100 ng/mL) or human IgG as the negative control.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565278/

    Tocilizumab can apply to immunodeficient mice (eg: SCID mice), Non-immunodeficient mice (eg: DBA/1J mice), various cancer cell lines and other in vitro assays (Only for Reference)

动物实验:
- 合并
  • Objective: Tocilizumab inhibits the growth of mucoepidermoid carcinomas
    Animal Models: Female severe combined immunodeficient (SCID) mice were transplanted with Michigan-Human Mucoepidermoid Carcinoma cells
    Formulation: --
    Dosages: 5 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/26287605

    Objective: Therapeutic assessment of Tocilizumab-Alendronate Conjugate in collagen-induced arthritis (CIA) mice model
    Animal Models: DBA/1j mice were injected with CII which emulsified in CFA or IFA
    Formulation: PBS
    Dosages: 0.8 mg/kg
    Administration: Locally injected on the arthritic ankle joints
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/28107624

    Tocilizumab can apply to immunodeficient mice (eg: SCID mice), Non-immunodeficient mice (eg: DBA/1J mice), various cancer cell lines and other in vitro assays (Only for Reference)

抗体信息

CAS No. ‎375823-41-9
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID