Trichostatin A (TSA)

目录号:S1045

Trichostatin A (TSA) Chemical Structure

Molecular Weight(MW): 302.4

Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。

规格 价格 库存 购买数量  
RMB 2653.01 现货
RMB 2022.93 现货
RMB 3660.93 现货
RMB 7125.3 现货
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客户使用该产品的5个实验数据:

  • Effect of TSA, SAHA and silibinin alone and in combination on left panel: IF staining for p-histone H3 Ser 10 and p-MPM-2 Ser /Thr positive cells and a-tubulin for mitotic spindle and DAPI as nuclear stain in H1299 cells. Right panel: % positive p-histone H3 Ser 10 mitotic cells and expression of p-MPM-2 Ser /Thr protein levels in H1299 cells.

    Epigenetics 2012 7(10):1161-72. Trichostatin A (TSA) purchased from Selleck.

    (B) The mRNA level of PRRT2 in the presence of different concentrations of DAC and TSA.

    Biochem Biophys Res Commun, 2017, 485(2):454-460. Trichostatin A (TSA) purchased from Selleck.

  • HCT116 p53 null cells were treated with different HDACIs (1 μM TSA, 5 μM M344, 1 μM MS-275, 5 mM But, 10 mM VPA) for 24 h, and their expression of GRP78, PERK-eIF2α axis and ATF4, ATF3, CHOP and DR5 proteins.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck.

    HCT116 p53 null cells were treated with different HDACIs (1 μM TSA, 5 μM M344, 1 μM MS-275, 5 mM But, 10 mM VPA) for 24 h. ATF4, ATF3, CHOP and DR5 proteins were measured by Western blot.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck.

  • Western blot analysis of Acetyl-H3 and H3. 0-20μM TSA was added.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Trichostatin A (TSA) purchased from Selleck.

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。
靶点
HDAC [1]
(Cell-free assay)
~1.8 nM
体外研究

Trichostatin A 抑制八种乳腺癌细胞系,包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖,平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系,抑制HDAC活性,平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同,Trichostatin A 抑制这些HDACs,且抑制程度相似, IC50 分别为6 nM, 38 nM, 和8.6 nM。[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞,通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体,而诱导转化生长因子βⅡ型受体(TβRII) 表达,复合体与TβRII 启动子的DNA片段结合,伴随着Sp1乙酰化,且与复合体相关的HDAC数理全部降低。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK 293 M12yNmZ2dmO2aX;uJGF{e2G7 MXOwMlfPxE1? MlHmNlRp M4\DUYV1cGGwb3y= Mm\S[Y5p[W6lZYOgSW5iSyCjY3X0fYxifGmxbjDhcoQhcW6lcnXhd4V{KEWQYVOgZYJ2dmSjbnPlJIlvKHSqZTD0c5RidCClZXzsJIx6e2G2ZTDhcoQh[XRidHjlJINmdGxic4Xy[oFk\Q>? MofHNlU4QDdyN{m=
TE13 MVPGeY5kfGmxbjDBd5NigQ>? MlHDNE4{|ryP NWHzN5VJOjSq NXH2cHA1fXBvcnXneYxifGW|IGLBV3NHPUFibHX2[Yw> MUmyOVU4QTZ4NR?=
TE13 NIDGeHdCeG:ydH;zbZMhSXO|YYm= MXWwMlPPxE1? NFH3cnczPGh? M1XuRYlvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25? NXXCVZhLOjV3N{m2OlU>
MEFs M2HldGZ2dmO2aX;uJGF{e2G7 M2HhSlXPxE1? NH:1WmkyPmh? NIPheVFqdmO{ZXHz[ZMhfGinIFXQSWMh[XS2YXPocYVvfCxiVHnyJIRmdGm4ZYL5JIFv\CC2aHWg[YZncWOrZX7jfUBw\iCyZXTld5RidCCob4LtZZRqd25? MUGyOVQ5OjZ|NB?=
SW480  NX3rUGluTnWwY4Tpc44hSXO|YYm= NXnYeFh[OC5zzszN MnHzOFhp NILpOVNFVVOR MkjidoV3\XK|ZYOgSW1V NV3M[XFbOjV2M{S5PVc>
PC3  NFnQZYpHfW6ldHnvckBCe3OjeR?= Mom3NE4y|ryP M1fV[VQ5cA>? NY\yRpd4TE2VTx?= MmPZdoV3\XK|ZYOgSW1V NHfXcGQzPTR|NEm5Oy=>
SW480  Ml3NSpVv[3Srb36gRZN{[Xl? NXvaU5FGOC5zzszN MmLsOFhp MX;EUXNQ MXLheJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? NEfTRoozPTR|NEm5Oy=>
PC3  NI\OUmVHfW6ldHnvckBCe3OjeR?= M1P2R|AvOc7:TR?= NGjqUpc1QGh? MW\EUXNQ M2DLXIF1fGWwdXH0[ZMhcW64YYPpc44h[W6mIH3p[5JifGmxbh?= NWDz[VJ[OjV2M{S5PVc>
SW480  MYTGeY5kfGmxbjDBd5NigQ>? NXexT3pLOC5zzszN NYDBPGk2PDiq M37LcmROW09? NGXBbXpqdmS3Y3XzJIlv[3KnYYPlJI9nKEiGQVOxJIFv\CCKRFHDNkBwdiCVbIXnJIdmdmW|IIDyc41wfGW{ NXHpUJNiOjV2M{S5PVc>
PC3  NFT4foNHfW6ldHnvckBCe3OjeR?= MlnENE4y|ryP M3f3UFQ5cA>? NYW4NodWTE2VTx?= MmPubY5lfWOnczDpcoNz\WG|ZTDv[kBJTEGFMTDhcoQhUESDQ{Kgc44hW2y3ZzDn[Y5meyCycn;tc5Rmeg>? NFfOVJIzPTR|NEm5Oy=>
A431 M17SNmFxd3C2b4Ppd{BCe3OjeR?= Mo[xNk8yOC93MD:xNFBvVQ>? NWnaSldbPDiq Mk\DSG1UVw>? NUj5VowzcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4To M4nCZVI2OzdzME[5
A431 MV;GeY5kfGmxbjDBd5NigQ>? NWHpdmN[PTCwTR?= Mn[yNk83NzF{L{K0bC=> MYHEUXNQ M1vvZ4FkfGm4YYTld{BxOjFiYX7kJIlvcGmkaYTzJGFVTjNiZYjwdoV{e2mxbh?= NYqxPXdLOjV|N{GwOlk>
MDA-MB-231 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rJXVAuPjBybl2= MoSyNlRp MWDEUXNQ NUG3S|M6UUN3MDDv[kAyODCwTR?= M{HZZVI2OTl{N{Kx
MCF7 NELtTG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HhWVAuPjBybl2= Mm\kNlRp M3nZS2ROW09? NFfORo5KSzVyIH;mJFc2dk1? NWrDS2lPOjVzOUK3NlE>
SKOV-3 NWPQe5BOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4noZ|EuOTEQvF2= NUDscnVvOjSq NY[4e4FsTE2VTx?= MX3JR|UxKG:oIEWuOu69VQ>? NU\5ZVRmOjVzNkm0PVE>
A549 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVyxMVEx|ryP M1n0fVI1cA>? MkDLSG1UVw>? MXHJR|UxKG:oIEOuNu69VQ>? NGOxdJUzPTF4OUS5NS=>
SKOV-3 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzFbmcxNjFvMd88US=> MlTaOFhp NUTRV25nTE2VTx?= Mli3TWM2OCCxZjCwMlfPxE1? MlLCNlUyPjl2OUG=
A549 NXr0ZpdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LEXVAvOS1zzszN NYP4b5JCPDiq MlvTSG1UVw>? M3rVdmlEPTBib3[gNE4zQM7:TR?= NV;2RolJOjVzNkm0PVE>
SKOV-3 NX;VOJU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LSXFAvODFvMD65{txO Mkm5O|Jp MWHEUXNQ MWnJR|UxKG:oIECuN|LPxE1? MXWyOVE3QTR7MR?=
A549 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\POnh2OC5yMT2wMlnPxE1? MmXxO|Jp NWfDeY5UTE2VTx?= M4iySmlEPTBib3[gNE4xPs7:TR?= MXKyOVE3QTR7MR?=
HeLa NULVZmt[TnWwY4Tpc44hSXO|YYm= MUiyOVBvVQ>? MVWxOog> NE\lb5BFVVOR NYTvV|FbcW6lcnXhd4V{KEO\UEHBNUBuWk6DIHX4dJJme3Orb39CpC=> MXSyOVEyPjZ6OB?=
CNE2 NH\3WXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HYZVExOC14MEDuUS=> NELpXIQzPC92OD:3Nog> NWHpXWczcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz MYOyOFk3QTlyMR?=
PC3 Ml\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUCxNFAuOTByMH7N MWWyOIg> NHLMU2tKSzVyIH;mJFMxOG6P MUSyOFg2PDZ3OB?=
LNCaP MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvZNVAxNTFyMEDuUS=> MVeyOIg> MXPJR|UxKG:oIEOwNI5O M1:0TVI1QDV2NkW4
HeLa  MnP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWK5PJhMOs7:TR?= MXS0PIg> Mke0[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHHic5V1KDJ3JR?= NXviTGY2OjR6NE[xN|U>
HMEC-1  MWTGeY5kfGmxbjDBd5NigQ>? M2[zc|MxOG6P M3zBd|I1cA>? MnL5bY5kemWjc3XzJIV5eHKnc4Ppc44hd2cEoG\FS2ZTO8LibWLORS=> MVmyOFcyODZ|MR?=
HeLa NG[yV41HfW6ldHnvckBCe3OjeR?= NGLOVYEydU1? M4XiNlAvPWh? M1SzeIFjd2yrc3jld{B1cGVidH;0ZYwhUESDQzDhZ5Rqfmm2eR?= MofpNlQ4ODd2N{S=
ACP02 NVnjO5NET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rGcVI2OC9|NUCvOVAxdk1? MVKyOIg> Mke2[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>? M1m2dFI1PjZ6NUS3
ACP03 M1Had2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXi1c25COjVyL{O1NE82ODCwTR?= MkLhNlRp MX\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlib3[gZZBxem:6aX3heIVtgSB5MNMgKUBifCB{NUFCpI5O MVKyOFY3QDV2Nx?=
U87 GBM M4XWVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlToNVAxNzNyMD:1NFAhdk1? MonLO|IhcA>? MXKxNFAmKGW2aHHuc4w> NYG0bXBIemWmdXPld{Bu\WGwIHPlcIwhdnWvYnXyJIJ6KDNzLDC1OEwh[W6mIEW4xsAm NEDSfmczPDR4NEi0NS=>
U87 GBM MoHESpVv[3Srb36gRZN{[Xl? NWLvOIV6OTByL{WwNEBPdQ>? MWG0PEBp NXvXZlVuOTByJTDleIhidm:u M4XHdGlv\HWlZYOgV4Vv\XOlZX7j[U1NcWunIFHseIVz[XSrb37zJIlvKE63Y3zlZZIhVW:{cHjvcI9ogQ>? MX:yOFQ3PDh2MR?=
RPE NHm3O3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPkcnJJOC5{L{CuOE8xNjhxMTFOwG0> NWLPTGxxOjRxNEivO|IhcA>? NIX3e3NFVVOR NIHpOlBqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBjgSClZXzsJIN6[2ynIHHydoV{fA>? M2DOTFI1PDV4NkCy
HT29  NVTHZ5VkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4L1dFE5OCCwTR?= M32zXFczKGh? NUiz[5RzUUN3MDDv[kAyQDBibl2= M33pVlI1OzZ6Mk[1
hMSCs NX;DNo13TnWwY4Tpc44hSXO|YYm= NGG4bIk3NjJ3IH7N NFvwOoQzPCCq MoPQSG1UVw>? M1HOepN1[WKrbHn6[ZMhUGm|dH;u[UBC[2W2eXzheIlwdiCjbnSgeIhmKEW6cILld5Nqd25ib3[gVIx2emmyb4TlcpQhT2WwZYO= MWOyOFMyOjN3Nh?=
Huh7  Ml:xSpVv[3Srb36gRZN{[Xl? NE\Xfm0xNjFxMD61M|EvOCEQvF2= NU\4NFVIOjSq M3HZW5N2eHC{ZYPz[ZMhYVSKRFOyJIdmdmViZYjwdoV{e2mxbh?= MlPkNlQzPjl4N{K=
SKOV3 Ml:1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvMRphjOC5yNT2yJO69VQ>? NX;aWW5jOTBxMkSvOFghcA>? MWLEUXNQ M1e0V41m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MYSyOFIzOzhyMR?=
A2780 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTENE4xPS1{IN88US=> MkHuNVAwOjRxNEigbC=> MYPEUXNQ MonTcYVlcWG2ZYOg[5Jwf3SqIHHydoV{fCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? MXqyOFIzOzhyMR?=
SRA01/04  M4PXcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYGwMlIh|ryP M3Xh[lQ5KGh? M3PIZWROW09? NX7D[VB6e3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? M3zTWlI1OTV5OEe4
HLEB3 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XXU|AvOiEQvF2= MXK0PEBp MX3EUXNQ MYnzeZBxemW|c3XzJINmdGxicILvcIln\XKjdHnvckB1cHKxdXfoJJN2eHC{ZYPzbY5oKHSqZTDQTVNMN0GtdDygdFM5VUGSSzDhcoQhTVKNMT:yJJNq\26jbHnu[{Bx[XSqd3H5dy=> NYX0[FI5OjRzNUe4O|g>
SRA01/04  M3n6XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknFNE41NzBwODFOwG0> MkLwOFghcA>? M1T2O2ROW09? NX7XeY0zcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NWToVlZPOjRzNUe4O|g>
HLEB3 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfGXW8xNjRxMD64JO69VQ>? MWi0PEBp NUDTRXl4TE2VTx?= NFXsXoJqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M3jU[VI1OTV5OEe4
HCT116 M3XHVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3Le2YxNjJizszN NV75TopYOTJiaB?= M3nIZoVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgdoFlcWG2aX;u NHOwTJIzPDF{MkKzNS=>
CA46 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjqeWU{NzZxMUKvNlQwPDhibl2= NIfLfXE1QCCq MVPpcohq[mm2czD0bIUh[2WubDDwdo9tcW[ncnH0bY9vKGG2IEK0JIFv\CB2ODDuUeKh M3zWbVI1ODZ2OUWx
PMNs NGK5N3JHfW6ldHnvckBCe3OjeR?= NHzqdoU{OCCwTR?= M1Ly[|Q5KGh? MlWwbY5pcWKrdIOg[YZn\XKxY4n0c5NqeyxiSFTBR{Bi[3Srdnn0fUBidmRiUnHjJIFkfGm4aYT5 NXTiZZM3OjN7OEi2NVc>
H1299 NFr6TppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfFOo9EOcLizszNxsA> NWnjOI1vOjRxNEivO|IhcA>? NIXOWphmfGijbn;s NH23XFJqdmO{ZXHz[ZMh[2WubDDk[YF1cCCjZoTldkAzPGh? M4HFflI{QTF4NkC5
A549 MlzDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHwNYZlOC5{NT:wMlUwOSEQvF2= NWT5UlV1OjRxNEivO|IhcA>? NIfhcJJmfGijbn;s MnO0bY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnuJINwdmOnboTyZZRqd25iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NIroR2szOzh4N{m5NS=>
H1299 Mon4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoH6NE4zPS9yLkWvNUDPxE1? NF;aO|czPC92OD:3NkBp MV;leIhidm:u M2fre4lvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDpckBkd26lZX70doF1cW:wIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NIHsNJozOzh4N{m5NS=>
A549 M{noOWFxd3C2b4Ppd{BCe3OjeR?= NYDpbVNTOC53L{Gg{txO M3m5NlQ5KGh? NFPUOHJmfGijbn;s NV7QPZdKcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MXOyN|g3Pzl7MR?=
H1299 NIO5cIdCeG:ydH;zbZMhSXO|YYm= NYLTTHQ6OC53L{Gg{txO MYS0PEBp NICxdpFmfGijbn;s MV3pcoR2[2W|IHPlcIwh[XCxcITvd4l{ NWPBRpJkOjN6Nke5PVE>
SUM149PT NIizTnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\ZfVIwPy53L{GwJO69VQ>? MnzPOFghcA>? NXjUbolNTE2VTx?= MXfpcoR2[2W|IHPlcIwh\GWjdHigZZQhOiEQvF2gbY5{\W6|aYTpeoVtgQ>? M4W0SFI{Pzl{NkO4
SUM190PT MlTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[1NE8yODBxMkWwJI5O NYDpOFBEPDhiaB?= MnP2SG1UVw>? M4TWbIlv\HWlZYOgZ4VtdCCmZXH0bEBifCB3MDDuUUB{\W6|aYTpeoVtgQ>? M{HPVFI{Pzl{NkO4
HCT1 Mn;iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVOwMlIwOS5yL{WuNEDPxE1? NWDV[ZRXOTJxMkSvN|YwPDhiaB?= MlfFbY5lfWOnczDj[YxtKGSnYYToJIlvKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NYjVOIF[OjN5N{CwNFA>
Lovo NUS5NnNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXybYUxNjJxMT6wM|UvOCEQvF2= NG\iZZgyOi9{ND:zOk81QCCq M{nWS4lv\HWlZYOgZ4VtdCCmZXH0bEBqdiClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MlnrNlM4PzByMEC=
AGS M{nHd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV:wMlAyPS1zIN88US=> MnfCO|IhcA>? MV7pcoR2[2W|IHPlcIwh\GWjdHigZ49v[2WwdILheIlwdiCmZYDlcoRmdnSueR?= NHf3O3kzOzd2NUCyOC=>
Huh7 NY[2ZnBOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XlZVEh|ryP Mo\LNlQhcA>? NXfncWpLTE2VTx?= M4jsSZJm\HWlZYOgZYJwfXRiMkClJJZq[WKrbHn0feKh Mnm4NlM3PDN7M{O=
ECC1 NIryeW5HfW6ldHnvckBCe3OjeR?= MYS1NFAhdk1? M{TUSVUh\A>? NYrFfWNpTE2VTx?= MmOwbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? MViyN|U{ODd4OR?=
HEC1A MUDGeY5kfGmxbjDBd5NigQ>? M4PvcVUxOCCwTR?= NE\zeYs2KGR? MYTEUXNQ Ml\4bY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? NUjsSWltOjN3M{C3Olk>
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U373 MX;GeY5kfGmxbjDBd5NigQ>? Mn;MNE4zPS9yLkWvNUDPxE1? NXruWZpUOjRiaB?= NYnRPVlLcW6lcnXhd4V{KFCULVKg[ZhxemW|c3nvckBkdy22cnXheI1mdnRid3n0bEA2SXqjZFO= MkWwNlM1PzRzN{G=
ARN8  NUjHS2hiTnWwY4Tpc44hSXO|YYm= NYXuVHlJOC5yNT2yJO69VQ>? NHPJTGUzPCCq MU\y[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=> NVmydY8yOjN2N{C1OFA>
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H1299  MlX3SpVv[3Srb36gRZN{[Xl? NUDPSHM2OC5|M,MAl|HDqML3TR?= M2T6[VI1NzR6IHi= NWTyS|JuTE2VTx?= M2HmVIlv[3KnYYPld{BGNWOjZHjldolvKHC{b4TlbY4hdGW4ZXzzJIRwe2ViZHXw[Y5l\W62bIm= MWiyN|Q3OTl5NR?=
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HeLa NU\FcGxTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEG4UY8yODBibl2= MXuyOEBp MWnEUXNQ NIXwTpVKSzVyIH;mJFExOG6P M4fDfVI{OTZ3N{S4
HeLa M3THR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETBdWI1OCCwTR?= MmH2OFghcA>? NVnBS|I{TE2VTx?= MoS1TWM2OCCxZjC0NI5O NHTxN3AzOzF4NUe0PC=>
HeLa NXnidFN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXWyNEBvVQ>? NFjvR5Y4OiCq M2XSdGROW09? MUXJR|UxKG:oIEKwcm0> Mk\HNlMyPjV5NEi=
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MCF-7  MkTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS3cmpYOjVvNECwJI5O MXy0PEBp NXXkUmk5UUN3MDDv[kAzOjBwNH7N MlXuNlMxPTVzOUi=
ECC-1  MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mme2NVAxKG6P NV\WeGRyOjRiaB?= NFfL[phmfGijbn;s NILrXXpqdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB|NTW= MnvCNlMxOjh6MEO=
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NHAC-kn Mlj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfQTIxKOTBxMUCwM|UxOCCwTR?= M3zEdlEzKGh? MYTEUXNQ MXXJR|UxKG:oIEWwNI5O NX;wd3N4OjNyMUe4O|E>
A549 M4jVe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoSzNlUxKG6P MUS2MVczKGh? NG\aU|VFVVOR NHu3TWdk[XW|ZYOgZUBoemWjdHXyJIlvcGmkaYTvdpkh\W[oZXP0JINwdWKrbnWge4l1cCCWWGSgc5Ih\XKub4Tpcolj M2Hpb|IzQTl2N{iw
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HL60  NHLCV5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXexOVAuOzVyIH7N MVqyOEBp MVfpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXQEoIfo[Y4h[2:wY3XueJJifGmxboOgbIlocGW{IIToZY4hOjVyIH7N M1HUflIzPzV|N{O5
U937 M1XhXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\OVZAyPTBvM{WwJI5O M13LdlI1KGh? NILVeohqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O NWS0XWFSOjJ5NUO3N|k>
SCC-6 Mlm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHFNlAxNTN{MECgcm0> NFLid|kyOi9{ND:0PEBp MVvEUXNQ MVjpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDv[kBUS0NvNjDj[YxteyCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? MX:yNlU2OjN{MR?=
U87  NE\hUnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjIflBsOTByLUOwNEBv\w>? NU\ZNo02OjRiaB?= M2i5c4lvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDD0c{A4OiViIHH0JFIxOG6p MV:yNlI4ODh2OR?=
K562 M1PwcGZ2dmO2aX;uJGF{e2G7 NGm1b4cyKM7:TR?= M4\mXVEzKGh? MYPEUXNQ NFO5N2hmdmijbnPld{B1cGViZYjwdoV{e2mxbjDv[kBTXU6[MzDpcoR2[2WmIHL5JFUu[XqjLVPkVi=> MmTzNlIyPzlzOUi=
Reh NFnhcJNHfW6ldHnvckBCe3OjeR?= NEO3bWoxNjNxMTFOwG0> NVT4NXlkOTJiaB?= M3Tzb2ROW09? MUjlcohidmOnczD0bIUh\XiycnXzd4lwdiCxZjDSWW5ZOyCrbnT1Z4VlKGK7IEWtZZpiNUOmUh?= NX\DU3RFOjJzN{mxPVg>

... Click to View More Cell Line Experimental Data

体内研究 Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型,持续处理4周,具有有效的抗癌活性,即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症(SMA)模型鼠,导致乙酰化的 H3 和 H4组蛋白水平提高,也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠,促进存活, 减轻体重下降,且增强运动行为。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
+ 展开

体外HDAC活性 :

从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时,或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×105 个细胞)与 1 μL ~1.5 × 106 cpm [3H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火,然后使用600 μL 乙酸乙酯进行抽提,通过闪烁计数器测量释放的[3H]乙酸。使用非线性回归曲线拟合抑制数据,绘制合适的剂量反应曲线,而测定IC50。
细胞实验:[1]
+ 展开
  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3
  • Concentrations: 溶于无水酒精,终浓度为~10 μM
  • Incubation Time: 96小时
  • Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。
    (Only for Reference)
动物实验:[1]
+ 展开
  • Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠
  • Formulation: 溶于DMSO
  • Dosages: ~5 mg/kg/day
  • Administration: 皮下注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次加入纯溶剂:
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 302.4
化学式

C17H22N2O3

CAS号 58880-19-6
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02959905 Recruiting Metastatic Melanoma BGI, China|Sun Yat-sen University December 2016 Phase 1
NCT02486965 Recruiting Fibromyalgia University Hospital, Brest October 2015 --
NCT01954433 Recruiting Glenohumeral Arthritis and/or Rotator Cuff Tear Arthropathy (TSA)|Rotator Cuff Tear (RCR)|Trapeziometacarpal Osteoarthritis (TMC OA) Matthias Flury|Winterthur Institute of Health Economics (WIG), Switzerland|Schulthess Klinik November 2013 --
NCT01575106 Unknown status Pain Massachusetts General Hospital June 2012 --
NCT02762903 Completed Osteoarthritis Columbia University August 2009 --
NCT00914173 Completed Pain Medasense Biometrics Ltd November 2008 --

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • 回答:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品

Tags: 购买Trichostatin A (TSA) | Trichostatin A (TSA)供应商 | 采购Trichostatin A (TSA) | Trichostatin A (TSA)价格 | Trichostatin A (TSA)生产 | 订购Trichostatin A (TSA) | Trichostatin A (TSA)代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID