Trichostatin A (TSA)

目录号:S1045

Trichostatin A (TSA) Chemical Structure

Molecular Weight(MW): 302.4

Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。

规格 价格 库存 购买数量  
RMB 2653.01 现货
RMB 2022.93 现货
RMB 3660.93 现货
RMB 7125.3 现货
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客户使用该产品的6个实验数据:

  • Gck expression in WT primary hepatocytes transduced with FOXO1 and KR-FOXO1 adenoviruses in the presence or absence of trichostatinA (TSA).

    Cell, 2017, 171(4):824-835.e18. Trichostatin A (TSA) purchased from Selleck.

    Effect of TSA, SAHA and silibinin alone and in combination on left panel: IF staining for p-histone H3 Ser 10 and p-MPM-2 Ser /Thr positive cells and a-tubulin for mitotic spindle and DAPI as nuclear stain in H1299 cells. Right panel: % positive p-histone H3 Ser 10 mitotic cells and expression of p-MPM-2 Ser /Thr protein levels in H1299 cells.

    Epigenetics 2012 7(10):1161-72. Trichostatin A (TSA) purchased from Selleck.

  • (B) The mRNA level of PRRT2 in the presence of different concentrations of DAC and TSA.

    Biochem Biophys Res Commun, 2017, 485(2):454-460. Trichostatin A (TSA) purchased from Selleck.

    HCT116 p53 null cells were treated with different HDACIs (1 μM TSA, 5 μM M344, 1 μM MS-275, 5 mM But, 10 mM VPA) for 24 h, and their expression of GRP78, PERK-eIF2α axis and ATF4, ATF3, CHOP and DR5 proteins.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck.

  • HCT116 p53 null cells were treated with different HDACIs (1 μM TSA, 5 μM M344, 1 μM MS-275, 5 mM But, 10 mM VPA) for 24 h. ATF4, ATF3, CHOP and DR5 proteins were measured by Western blot.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.01.184. Trichostatin A (TSA) purchased from Selleck.

    Western blot analysis of Acetyl-H3 and H3. 0-20μM TSA was added.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Trichostatin A (TSA) purchased from Selleck.

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。
靶点
HDAC [1]
(Cell-free assay)
~1.8 nM
体外研究

Trichostatin A 抑制八种乳腺癌细胞系,包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖,平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系,抑制HDAC活性,平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同,Trichostatin A 抑制这些HDACs,且抑制程度相似, IC50 分别为6 nM, 38 nM, 和8.6 nM。[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞,通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体,而诱导转化生长因子βⅡ型受体(TβRII) 表达,复合体与TβRII 启动子的DNA片段结合,伴随着Sp1乙酰化,且与复合体相关的HDAC数理全部降低。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK 293 MoniSpVv[3Srb36gRZN{[Xl? NXfYblE6OC55zszN NWDIXmVjOjSq NX;udVJT\XSqYX7vcC=> NXrGbXFD\W6qYX7j[ZMhTU6jQzDhZ4V1gWyjdHnvckBidmRiaX7jdoVie2W|IFXOZWMh[WK3bnThcoNmKGmwIITo[UB1d3SjbDDj[YxtKGy7c3H0[UBidmRiYYSgeIhmKGOnbHygd5Vz\mGlZR?= M4P5[lI2Pzh5MEe5
TE13 NUTRPI1WTnWwY4Tpc44hSXO|YYm= MmXPNE4{|ryP MYKyOIg> NYDDcJU1fXBvcnXneYxifGW|IGLBV3NHPUFibHX2[Yw> MVOyOVU4QTZ4NR?=
TE13 M{DnN2Fxd3C2b4Ppd{BCe3OjeR?= NX61VYtEOC5|zszN M3m1flI1cA>? NULHPYt5cW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbh?= NGe4[|MzPTV5OU[2OS=>
MEFs M1\xZ2Z2dmO2aX;uJGF{e2G7 M3\DfFXPxE1? MkfWNVZp NG\hd3RqdmO{ZXHz[ZMhfGinIFXQSWMh[XS2YXPocYVvfCxiVHnyJIRmdGm4ZYL5JIFv\CC2aHWg[YZncWOrZX7jfUBw\iCyZXTld5RidCCob4LtZZRqd25? Mk[0NlU1QDJ4M{S=
SW480  NXjubYYxTnWwY4Tpc44hSXO|YYm= NIXWdpgxNjIQvF2= M3raSFQ5cA>? MljFSG1UVw>? MkSxdoV3\XK|ZYOgSW1V NWrMRWdXOjV2M{S5PVc>
PC3  NF7PV4RHfW6ldHnvckBCe3OjeR?= NFG1Zm0xNjIQvF2= NWftelRMPDiq NWrSNFd5TE2VTx?= MUXy[ZZmenOnczDFUXQ> MX:yOVQ{PDl7Nx?=
SW480  Mke5SpVv[3Srb36gRZN{[Xl? MkLRNE4y|ryP M1zodVQ5cA>? M37yR2ROW09? NYLHWXpt[XS2ZX71ZZRmeyCrbo\hd4lwdiCjbnSgcYloemG2aX;u NH7CXoUzPTR|NEm5Oy=>
PC3  MV;GeY5kfGmxbjDBd5NigQ>? MUCwMlHPxE1? NXnmV3F1PDiq NV24WIx[TE2VTx?= MU\heJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? MVuyOVQ{PDl7Nx?=
SW480  M3jDOGZ2dmO2aX;uJGF{e2G7 MmfWNE4y|ryP NVjTNGtvPDiq NELwUFBFVVOR NH;pdGhqdmS3Y3XzJIlv[3KnYYPlJI9nKEiGQVOxJIFv\CCKRFHDNkBwdiCVbIXnJIdmdmW|IIDyc41wfGW{ NX\hPI84OjV2M{S5PVc>
PC3  NIX4b2tHfW6ldHnvckBCe3OjeR?= M{DDZ|AvOc7:TR?= NVXSVplNPDiq MX3EUXNQ NXLFXJV[cW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?= MnnzNlU1OzR7OUe=
A431 NVLZUXk3SXCxcITvd4l{KEG|c3H5 MYGyM|ExNzVyL{GwNI5O MUm0PIg> MlTYSG1UVw>? NGXHXnZqdmirYnn0d{B1cGViY3XscEBoem:5dHi= NFvFOWgzPTN5MUC2PS=>
A431 NVTsWI1pTnWwY4Tpc44hSXO|YYm= Mn63OVBvVQ>? MYmyM|YwOTJxMkTo MXXEUXNQ M17FXIFkfGm4YYTld{BxOjFiYX7kJIlvcGmkaYTzJGFVTjNiZYjwdoV{e2mxbh?= MUOyOVM4OTB4OR?=
MDA-MB-231 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWOwMVYxOG6P Mmn4NlRp MUnEUXNQ NHHKSlNKSzVyIH;mJFExOG6P NXG5W3lSOjVzOUK3NlE>
MCF7 NFnVeo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\DRVAuPjBybl2= NITRdXMzPGh? MoPuSG1UVw>? NUPGXI82UUN3MDDv[kA4PW6P M2nwd|I2OTl{N{Kx
SKOV-3 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHaNU0yOM7:TR?= MkHuNlRp NHPJdIpFVVOR M4PpXWlEPTBib3[gOU43|ryP NYLwZ|J5OjVzNkm0PVE>
A549 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrxdnlnOS1zMN88US=> NF;PXWwzPGh? M1jFdGROW09? NYG0blN7UUN3MDDv[kA{NjMQvF2= M3LLZlI2OTZ7NEmx
SKOV-3 NIDZcYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXVNE4yNTIQvF2= NXHM[4x6PDiq NUHHOXZZTE2VTx?= NVe4S5Q3UUN3MDDv[kAxNjgQvF2= MV:yOVE3QTR7MR?=
A549 M13LXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf6NoExNjFvMd88US=> MmnoOFhp NYC1RZFuTE2VTx?= MXHJR|UxKG:oIECuNljPxE1? NF70UJQzPTF4OUS5NS=>
SKOV-3 NHXvUXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzJNE4xOS1yLkpOwG0> MnvXO|Jp M{\TeWROW09? M{TZNWlEPTBib3[gNE4{Os7:TR?= NHP2PHYzPTF4OUS5NS=>
A549 MnjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXP0RWZLOC5yMT2wMlnPxE1? M3T1SFczcA>? NETHS|JFVVOR NV;qd2ZKUUN3MDDv[kAxNjB4zszN M2Ttc|I2OTZ7NEmx
HeLa Ml\lSpVv[3Srb36gRZN{[Xl? M4nGU|I2OG6P M4XnbVE3cA>? NI\QXlRFVVOR M4L4Zolv[3KnYYPld{BEYVBzQUGgcXJPSSCneIDy[ZN{cW:wwrC= MkT4NlUyOTZ4OEi=
CNE2 NG\1bmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4[xNlExOC14MEDuUS=> MYiyOE81QC95Mni= MlO2bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Ml72NlQ6Pjl7MEG=
PC3 NGLINVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\hWYpOOTByLUGwNFBvVQ>? NED6dGIzPGh? MlTsTWM2OCCxZjCzNFBvVQ>? MViyOFg2PDZ3OB?=
LNCaP MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIf2cFEyODBvMUCwNI5O MVKyOIg> M4j0XWlEPTBib3[gN|Axdk1? MXuyOFg2PDZ3OB?=
HeLa  M3HBWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXqy{txO MlfYOFhp M{\Fd4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDhZo92fCB{NTW= M2HjNlI1QDR4MUO1
HMEC-1  NUTNbGlXTnWwY4Tpc44hSXO|YYm= NWPyXJU2OzBybl2= MVKyOIg> NYWyOoRDcW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>? NH62cXQzPDdzME[zNS=>
HeLa MkDxSpVv[3Srb36gRZN{[Xl? MkPHNY1O M160flAvPWh? MWPhZo9tcXOqZYOgeIhmKHSxdHHsJGhFSUNiYXP0bZZqfHl? NUSxTm9lOjR5MEe0O|Q>
ACP02 NEfYWnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPEcpYzPTBxM{WwM|UxOG6P MXuyOIg> NFruXHBl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P NG\ENI0zPDZ4OEW0Oy=>
ACP03 NHvxZo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHFNlUxNzN3MD:1NFBvVQ>? MoG0NlRp M2OwS4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1? NWHGc|NqOjR4Nki1OFc>
U87 GBM MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojENVAxNzNyMD:1NFAhdk1? NFT4Z3Q4OiCq MXWxNFAmKGW2aHHuc4w> MmnmdoVlfWOnczDt[YFvKGOnbHygcpVu[mW{IHL5JFMyNCB3NDygZY5lKDV6wrCl MlvSNlQ1PjR6NEG=
U87 GBM MkDlSpVv[3Srb36gRZN{[Xl? MYWxNFAwPTByIF7t MYm0PEBp NHHlWHMyODBnIHX0bIFvd2x? NGmz[m9KdmS3Y3XzJHNmdmW|Y3XuZ4UuVGmtZTDBcJRmemG2aX;ud{BqdiCQdXPs[YFzKE2xcoDoc4xw\3l? NWjwXJJjOjR2NkS4OFE>
RPE NGjpZZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF[y[VAxNjJxMD60M|AvQC9zIN88US=> M1\XSlI1NzR6L{eyJIg> MYDEUXNQ NWmxO3pxcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R? NUTLdlYyOjR2NU[2NFI>
HT29  NGqzRXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3v1d|E5OCCwTR?= MVG3NkBp NFnOVWlKSzVyIH;mJFE5OCCwTR?= NGe1O3YzPDN4OEK2OS=>
hMSCs M1TsdGZ2dmO2aX;uJGF{e2G7 NGDGXm83NjJ3IH7N MkDPNlQhcA>? M4i3RWROW09? NYLBfHZte3SjYnnsbZpmeyCKaYP0c45mKEGlZYT5cIF1cW:wIHHu[EB1cGViRYjwdoV{e2mxbjDv[kBRdHW{aYDveIVvfCCJZX7ldy=> NFy3cWszPDNzMkO1Oi=>
Huh7  NFTaOZdHfW6ldHnvckBCe3OjeR?= MoS0NE4yNzBwNT:xMlAh|ryP NWXSdnp[OjSq NWfaV|F6e3WycILld5NmeyC\VFjER|Ih\2WwZTDlfJBz\XO|aX;u Mni5NlQzPjl4N{K=
SKOV3 M2[3V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLLcoNJOC5yNT2yJO69VQ>? MmPqNVAwOjRxNEigbC=> Mn;CSG1UVw>? M2nFXI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NGfEU4szPDJ{M{iwNS=>
A2780 MmLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnIVXZGOC5yNT2yJO69VQ>? M37udFExNzJ2L{S4JIg> MnjBSG1UVw>? MU\t[YRq[XSnczDndo94fGhiYYLy[ZN1KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MkXqNlQzOjN6MEG=
SRA01/04  MlTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjXNE4zKM7:TR?= MormOFghcA>? MVTEUXNQ NVnaenFwe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? NEfyXpMzPDF3N{i3PC=>
HLEB3 NGi1XphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPyRVUxNjJizszN MXe0PEBp MmXiSG1UVw>? NFHFdFR{fXCycnXzd4V{KGOnbHygdJJwdGmoZYLheIlwdiC2aILveYdpKHO3cIDy[ZN{cW6pIITo[UBRUTONL1HreEwheDN6TVHQT{BidmRiRWLLNU8zKHOrZ37hcIlv\yCyYYToe4F6ew>? NGXUU3QzPDF3N{i3PC=>
SRA01/04  MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fIclAvPC9yLkig{txO NWSxRWY{PDhiaB?= NXvTNph[TE2VTx?= MUTpcoR2[2W|IHPlcIwh[XCxcITvd4l{ NV7RVGY6OjRzNUe4O|g>
HLEB3 M1PhWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjsNHYxNjRxMD64JO69VQ>? NVXGd2F7PDhiaB?= NIjKPFJFVVOR NYPWR2JDcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MoDtNlQyPTd6N{i=
HCT116 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUX5OVZMOC5{IN88US=> M2npPFEzKGh? MlLX[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDyZYRq[XSrb36= M2PiZVI1OTJ{MkOx
CA46 MofqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XWfFMwPi9zMj:yOE81QCCwTR?= M1ra[|Q5KGh? NUHJbZlbcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDheEAzPCCjbnSgOFghdk4EoB?= Ml\PNlQxPjR7NUG=
PMNs NUnJ[IlSTnWwY4Tpc44hSXO|YYm= M2\4W|MxKG6P NFfXSnM1QCCq Mn3xbY5pcWKrdIOg[YZn\XKxY4n0c5NqeyxiSFTBR{Bi[3Srdnn0fUBidmRiUnHjJIFkfGm4aYT5 MXyyN|k5QDZzNx?=
H1299 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo[3NeKh|ryPwrC= MlvPNlQwPDhxN{KgbC=> M2P1XoV1cGGwb3y= MnHvbY5kemWjc3XzJINmdGxiZHXheIgh[W[2ZYKgNlRp M2rmPVI{QTF4NkC5
A549 NVLObGxIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUSwMlI2NzBwNT:xJO69VQ>? M{juZVI1NzR6L{eyJIg> M4TaeoV1cGGwb3y= NGD6NGJqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY4h[2:wY3XueJJifGmxbjDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MknONlM5Pjd7OUG=
H1299 NV[1OW15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFq5WJMxNjJ3L{CuOU8yKM7:TR?= NXz2fItbOjRxNEivO|IhcA>? M1rWZ4V1cGGwb3y= Mn33bY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnuJINwdmOnboTyZZRqd25iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MoDTNlM5Pjd7OUG=
A549 MUjBdI9xfG:|aYOgRZN{[Xl? NHLRS2UxNjVxMTFOwG0> M{j4cVQ5KGh? NUHETJVk\XSqYX7vcC=> MnntbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MWWyN|g3Pzl7MR?=
H1299 NFf0XZRCeG:ydH;zbZMhSXO|YYm= NEjqT4YxNjVxMTFOwG0> NVe5WmhSPDhiaB?= MYjleIhidm:u NULYO21ZcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MWOyN|g3Pzl7MR?=
SUM149PT MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\1dnNROi95LkWvNVAh|ryP MnHzOFghcA>? MUPEUXNQ NFPiWGdqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= NE\tS44zOzd7Mk[zPC=>
SUM190PT NELtZ4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DzbFUxNzFyMD:yOVAhdk1? NYrQSolVPDhiaB?= NGLXXFdFVVOR MUPpcoR2[2W|IHPlcIwh\GWjdHigZZQhPTBibl2gd4Vve2m2aY\lcJk> NVnvfXJFOjN5OUK2N|g>
HCT1 NITMNVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnK1NE4zNzFwMD:1MlAh|ryP MlPpNVIwOjRxM{[vOFghcA>? NUfkbodycW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M4nSRVI{PzdyMECw
Lovo NGHqeZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvwdJdNOC5{L{GuNE82NjBizszN NUDrclFsOTJxMkSvN|YwPDhiaB?= NYLkWYlzcW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M3W5TlI{PzdyMECw
AGS MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DXZVAvODF3LUGg{txO NGntZpY4OiCq MnqwbY5lfWOnczDj[YxtKGSnYYToJINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= Ml22NlM4PDVyMkS=
Huh7 Mo\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjnUI5NOSEQvF2= NUW3ZnNZOjRiaB?= MoXESG1UVw>? MkS4doVlfWOnczDhZo92fCB{MDWgeoli[mmuaYT5xsA> MWCyN|Y1Ozl|Mx?=
ECC1 NH;nUZNHfW6ldHnvckBCe3OjeR?= NFn0Z3c2ODBibl2= M1nkbVUh\A>? NXX0[VdpTE2VTx?= MWnpcoR2[2W|IFyxR2FOyqCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEWtRZpiSw>? MXWyN|U{ODd4OR?=
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U373 NHnr[GJHfW6ldHnvckBCe3OjeR?= NUn1[|NPOC5{NT:wMlUwOSEQvF2= NF;OZ|gzPCCq NIHwOotqdmO{ZXHz[ZMhWFJvQjDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFVCgmGmQx?= M3TpWVI{PDd2MUex
ARN8  NVnHW2JwTnWwY4Tpc44hSXO|YYm= Mn21NE4xPS1{IN88US=> M2rWWFI1KGh? Mm\BdoVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIIC1N{1l\XCnbnTlcpQh\2WwZYOgZpkhVnW2bHnuMVM> MlrGNlM1PzB3NEC=
MCF7 NWLX[HRUTnWwY4Tpc44hSXO|YYm= M1PZbFAvODVvMjFOwG0> NW[xOYxSOjRiaB?= MkjidoVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIIC1N{1l\XCnbnTlcpQh\2WwZYOgZpkhVnW2bHnuMVM> MXqyN|Q4ODV2MB?=
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H1299 NEnQdWJHfW6ldHnvckBCe3OjeR?= MkHONE42KM7:TR?= MX[0PEBp NF;jTGpFVVOR MV3pcohq[mm2czD0bIUhcW64YYPpeoVv\XO|wrCgZ49u[mmwZTD3bZRpKHOrbHnibY5qdg>? M33vOFI{PDZzOUe1
MG-63  NUnmO5hFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDKdZgxNjNizszN M2nQXVEzNTl4IHi= NVLvV5ExTE2VTx?= M4mwOIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDjc{11emWjdH3lcpQhf2m2aDDt[ZRnd3KvaX6= Mn\1NlM1PTF6MUe=
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K562 NF;odW9HfW6ldHnvckBCe3OjeR?= NHu5ZWsxNjVizszN NEXhO4wzPCCq NX\BS2k6\XSqYX7vcC=> M2jDOYRm[3KnYYPld{B1cGViZX76fY1mKGGldHn2bZR6KG:oIFjERWN{KGOxLYTy[YF1dWWwdDD3bZRpKGO3cnP1cYlv NHnBVWgzOzR|MEm1Oy=>
HEL MlewSpVv[3Srb36gRZN{[Xl? MVGwMlUh|ryP MojXNlQhcA>? Mn64[ZRp[W6xbB?= MlTM[IVkemWjc3XzJJRp\SCnbor5cYUh[WO2aY\peJkhd2ZiSFTBR5Mh[29vdILlZZRu\W62IIfpeIgh[3W{Y4XtbY4> MV2yN|Q{ODl3Nx?=
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Kazumi MoHxRZBweHSxc3nzJGF{e2G7 NGLq[YUyKM7:TR?= NUDk[2d4OjRiaB?= NUPkfHc4cW6mdXPld:Kh[2WubDDk[YF1cA>? NXL1PYFzOjN2MEC1NVk>
K562 M{nZ[mFxd3C2b4Ppd{BCe3OjeR?= NHryUWgyKM7:TR?= NV76fWdZOjRiaB?= M3Gwc4lv\HWlZYRCpINmdGxiZHXheIg> NFnJeHAzOzRyMEWxPS=>
THP1 MkPVRZBweHSxc3nzJGF{e2G7 NVv4VHo2OSEQvF2= NYnkU3A4OjRiaB?= NVWzPIhUcW6mdXPld:Kh[2WubDDk[YF1cA>? M2CzRVI{PDByNUG5
SH-SY5Y NUPlW3FTTnWwY4Tpc44hSXO|YYm= NXfBVJF3OjVyIH7N M{LNTlE3KGh? M3nJbINp[W6pZYOg[ZhxemW|c3nvckBx[XS2ZYLuJI9nKGenbnXzJIlvfm:udnXkJIlvKGOqb3zld5Rmem:uIIP5cpRp\XOrczygeZB1[WunIHHu[EBm\m[udYi= MX:yN|MzPjR{Mh?=
HEK293 Mk\OSpVv[3Srb36gRZN{[Xl? NY\yc5poOSEEtV5CpC=> M{fyNlE5KGkEoB?= NInWWmlqdmirYnn0d{B1cGVia3HsbZJqdi15LX3l[IlifGWmIILlZ5J2cXSvZX70JI9nKHO7boDobYxqdi1zIHHn[5Jm\2G2ZYOgbY51dyCjZ3fy[ZNwdWW| M4SwZVI{Ojh2OES4
HTK M2\u[mZ2dmO2aX;uJGF{e2G7 MX[0NFAhdk1? NHvMOIc4OiCq NIjZ[WdqdmirYnn0d{BVT0ZvzsNihLNKdmS3Y3XkJG16d2[rYoLvZoxie3RiRHnm[oVz\W62aXH0bY9v NIi1fmwzOzJ6NECwNi=>
HTK M{jzdmZ2dmO2aX;uJGF{e2G7 M2rze|ExOC16MEDuUS=> NIDJd|I4OiCq M4jBSIJtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w MnPsNlMzQDRyMEK=
Caco-2  M1zEZmZ2dmO2aX;uJGF{e2G7 M1;FRlEhyrWPwrC= M1\6T|I1KGh? MXvk[YNz\WG|ZYOgV2VTXCCycn;0[YlvKGW6cILld5Nqd25? NELFWZYzOzF7NUC3NC=>
HeLa MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrXNVAxKG6P NWW4Z5RROjRiaB?= NFyzZYxFVVOR M4HmN2lEPTBib3[gNVAxdk1? MlLxNlMyPjV5NEi=
HeLa NXT0N3l6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXhSm01OCCwTR?= Ml7SOFghcA>? NXTLT4pETE2VTx?= NEjaZo9KSzVyIH;mJFQxdk1? NGHZNFAzOzF4NUe0PC=>
HeLa M2HibGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\lT3pXOjBibl2= NHPJRVE4OiCq MlnVSG1UVw>? MkHKTWM2OCCxZjCyNI5O NEXDRoUzOzF4NUe0PC=>
HeLa NGLve5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTSZ|JGOTBxM{CvOVAhdk1? NUP2[nNmPzJiaB?= M4DrWWROW09? MVPpcoNz\WG|ZYOgeIhmKG63bXLldkBw\iCPTWCgLO6V|qivKTDsc5N{KGOnbHzzJIRwe2ViZHXw[Y5l\W62bIm= Mk\UNlMyPjV5NEi=
MDA-MB-231 M{XWWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDiNlUuPDByIH7N NXj1VJh6PDhiaB?= M4fPWWlEPTBib3[gNlY{NjKwTdMg M{HSc|I{ODV3MUm4
MCF-7  MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVqyOU01ODBibl2= NHrrWoo1QCCq M4ezXmlEPTBib3[gNlIxNjSwTR?= MnfBNlMxPTVzOUi=
ECC-1  MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXm5OIo3OTByIH7N NYSwVZl5OjRiaB?= M4LWcIV1cGGwb3y= NVLUbmNrcW6lcnXhd4V{KHSqZTDwdo9xd3K2aX;uJI9nKGGyb4D0c5Rq[yCwdXPs[YkhfG9iM{Wl M2rZblI{ODJ6OECz
HEC-1A MlXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrZVoYyODBibl2= MYmyOEBp NX7uXlJy\XSqYX7vcC=> M3XBXIlv[3KnYYPld{B1cGVicILvdI9zfGmxbjDv[kBieG:ydH;0bYMhdnWlbHXpJJRwKDN7JR?= NYnsXohYOjNyMki4NFM>
NHAC-kn MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPhNVAwOTByL{WwNEBvVQ>? MVuxNkBp M{O2fmROW09? M1S4XWlEPTBib3[gOVAxdk1? NF\PSWQzOzBzN{i3NS=>
A549 NGK2bZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn1Z5JbOjVyIH7N MWi2MVczKGh? NFHCdlNFVVOR MXXjZZV{\XNiYTDndoVifGW{IHnubIljcXSxcomg[YZn\WO2IHPvcYJqdmVid3n0bEBVYFRib4Kg[ZJtd3Srbnni M4LBU|IzQTl2N{iw
MG-63 NIrHNJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HL[|MxOCCwTR?= NUPobmdFOTJiaB?= M3TSXWROW09? MX3pcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gPFYm M3fESlIzPzl7M{O4
MG-63 NUnJVpNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\QVJVPOzByIH7N MnzWNlQhcA>? NYPBbodETE2VTx?= M4LsUIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDD0c{A3PyV? M4DibVIzPzl7M{O4
MG-63 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3O5UVMxOCCwTR?= MVy0PEBp NHvzS5FFVVOR MWrpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gOVYm NH7GNJIzOjd7OUOzPC=>
HL60  MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3G4b|E2OC1|NUCgcm0> NFvlTpQzPCCq M33vUolv[3KnYYPld{Bk\WyuIHHwc5B1d3Orc9Mge4hmdiClb37j[Y51emG2aX;ud{BpcWeqZYKgeIhidiB{NUCgcm0> Ml;NNlI4PTN5M{m=
U937 NHL0cFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPzeox7OTVyLUO1NEBvVQ>? Mk\NNlQhcA>? NH\aTpFqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O NFXMVJgzOjd3M{ezPS=>
SCC-6 NHvZXIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXQSWozODBvM{KwNEBvVQ>? M2XTdFEzNzJ2L{S4JIg> NG\GTWpFVVOR MmmxbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gc4YhW0OFLU[gZ4VtdHNiaX6gZUBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NVfOfYM5OjJ3NUKzNlE>
U87  M13PNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLnNVAxNTNyMDDu[y=> MV[yOEBp NWfyeVBscW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJJRwKDd{JTCgZZQhOjBybne= NYrzb4l7OjJ{N{C4OFk>
K562 NHmxbo9HfW6ldHnvckBCe3OjeR?= NYi0[ZA4OSEQvF2= M2[3elEzKGh? NFXDUZVFVVOR NXHJN|l1\W6qYX7j[ZMhfGinIHX4dJJme3Orb36gc4YhWlWQWEOgbY5lfWOnZDDifUA2NWG8YT3D[HI> NVq3TIdyOjJzN{mxPVg>
Reh NXHmfWpzTnWwY4Tpc44hSXO|YYm= NInxZmsxNjNxMTFOwG0> NIPBS5oyOiCq MojiSG1UVw>? Mor2[Y5p[W6lZYOgeIhmKGW6cILld5Nqd25ib3[gVnVPYDNiaX7keYNm\CCkeTC1MYF7[S2FZGK= MUSyNlE4QTF7OB?=

... Click to View More Cell Line Experimental Data

体内研究 Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型,持续处理4周,具有有效的抗癌活性,即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症(SMA)模型鼠,导致乙酰化的 H3 和 H4组蛋白水平提高,也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠,促进存活, 减轻体重下降,且增强运动行为。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
+ 展开

体外HDAC活性 :

从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时,或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×105 个细胞)与 1 μL ~1.5 × 106 cpm [3H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火,然后使用600 μL 乙酸乙酯进行抽提,通过闪烁计数器测量释放的[3H]乙酸。使用非线性回归曲线拟合抑制数据,绘制合适的剂量反应曲线,而测定IC50。
细胞实验:[1]
+ 展开
  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3
  • Concentrations: 溶于无水酒精,终浓度为~10 μM
  • Incubation Time: 96小时
  • Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。
    (Only for Reference)
动物实验:[1]
+ 展开
  • Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠
  • Formulation: 溶于DMSO
  • Dosages: ~5 mg/kg/day
  • Administration: 皮下注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用:
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 302.4
化学式

C17H22N2O3

CAS号 58880-19-6
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02959905 Recruiting Metastatic Melanoma BGI, China|Sun Yat-sen University December 2016 Phase 1
NCT02486965 Recruiting Fibromyalgia University Hospital, Brest October 2015 --
NCT01954433 Recruiting Glenohumeral Arthritis and/or Rotator Cuff Tear Arthropathy (TSA)|Rotator Cuff Tear (RCR)|Trapeziometacarpal Osteoarthritis (TMC OA) Matthias Flury|Winterthur Institute of Health Economics (WIG), Switzerland|Schulthess Klinik November 2013 --
NCT01575106 Unknown status Pain Massachusetts General Hospital June 2012 --
NCT02762903 Completed Osteoarthritis Columbia University August 2009 --
NCT00914173 Completed Pain Medasense Biometrics Ltd November 2008 --

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • 回答:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品

Tags: 购买Trichostatin A (TSA) | Trichostatin A (TSA)供应商 | 采购Trichostatin A (TSA) | Trichostatin A (TSA)价格 | Trichostatin A (TSA)生产 | 订购Trichostatin A (TSA) | Trichostatin A (TSA)代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID