Cyclosporin A (NSC 290193)

For research use only. Not for use in humans.

目录号：S2286 别名： Cyclosporine A, Cyclosporine, Ciclosporin, CsA 中文名称：环孢菌素A

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂，与亲环素结合，随后抑制calcineurin，无细胞试验中IC50为7 nM，广泛用于预防器官移植反应中的排斥反应。

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价格

库存

购买数量

10mM (1mL in DMSO)	RMB 1073.15	现货
50mg	RMB 786.07	现货
200mg	RMB 1957.41	现货
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客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献60篇：

Cancer Cell, 2018, 33(3):480-494

PubMed: 29533786 ( click the link to review the publication )

Theranostics, 2022, 12(4): 1621-1638

PubMed: N/A ( click the link to review the publication )

Theranostics, 2022, 12(3):1148-1160

PubMed: 35154479 ( click the link to review the publication )

Cell Rep, 2021, 35(7):109159

PubMed: 34010655 ( click the link to review the publication )

Protein Cell, 2021, 10.1007/s13238-021-00885-0

PubMed: 34677780 ( click the link to review the publication )

PLoS Biol, 2021, 19(4):e3001134

PubMed: 33901180 ( click the link to review the publication )

Cell Death Dis, 2021, 12(11):1050

PubMed: 34741026 ( click the link to review the publication )

Int J Biol Sci, 2021, 17(13):3672-3688

PubMed: 34512174 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(14)7540

PubMed: 34299155 ( click the link to review the publication )

Exp Neurol, 2021, 338:113598

PubMed: 33422553 ( click the link to review the publication )

J Cell Mol Med, 2021, 25(12):5729-5743

PubMed: 33949118 ( click the link to review the publication )

Front Pharmacol, 2021, 12:706901

PubMed: 34483914 ( click the link to review the publication )

Phytomedicine, 2021, 85:153551

PubMed: 33827043 ( click the link to review the publication )

Br J Nutr, 2021, 1-11

PubMed: 33663624 ( click the link to review the publication )

Chem Biol Interact, 2021, 351:109756

PubMed: 34808100 ( click the link to review the publication )

Peptides, 2021, S0196-9781(21)00117-0

PubMed: 34242679 ( click the link to review the publication )

Clin Transl Med, 2021, 11(4):e390

PubMed: 33931967 ( click the link to review the publication )

Mol Cell, 2021, S1097-2765(21)00087-3

PubMed: 33626321 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2021, 414:115426

PubMed: 33524445 ( click the link to review the publication )

Anal Chem, 2020, 10.1021/acs.analchem.0c02756

PubMed: 32933243 ( click the link to review the publication )

Free Radic Biol Med, 2020, 150:109-119

PubMed: 32105828 ( click the link to review the publication )

Toxicol In Vitro, 2020, 8;66:104857

PubMed: 32278034 ( click the link to review the publication )

J Fungi (Basel), 2020, 6(3):E103

PubMed: 32650564 ( click the link to review the publication )

Circulation, 2019, 10.1161/CIRCULATIONAHA.118.037227

PubMed: 31163979 ( click the link to review the publication )
J Clin Invest, 2019, 129(6):2431-2445

PubMed: 31081803 ( click the link to review the publication )

Autophagy, 2019, 15(3):493-509

PubMed: 30304977 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-19-1982

PubMed: 31582380 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):487

PubMed: 31822296 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2019, 14:192-203

PubMed: 30623853 ( click the link to review the publication )

Front Immunol, 2019, 10:2952

PubMed: 31921197 ( click the link to review the publication )

J Antimicrob Chemother, 2019, 74(7):1921-1927

PubMed: 30934052 ( click the link to review the publication )

Oxid Med Cell Longev, 2019, 2019:4248529

PubMed: 30881590 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(10):6897-6906

PubMed: 31379115 ( click the link to review the publication )

Front Microbiol, 2019,

PubMed: 32038504 ( click the link to review the publication )

J Med Virol, 2019, 91(8):1440-1447

PubMed: 30900754 ( click the link to review the publication )

Med Sci Monit, 2019,

PubMed: 30913205 ( click the link to review the publication )

Biochim Biophys Acta Gene Regul Mech, 2019, 1862(8):846-857

PubMed: 31054961 ( click the link to review the publication )

Nat Commun, 2018, 9(1):873

PubMed: 29491374 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(23):6066-6077

PubMed: 30061362 ( click the link to review the publication )

Oncogenesis, 2018, 7(11):91

PubMed: 30467308 ( click the link to review the publication )

Sci Rep, 2018, 8(1):10910

PubMed: 30026610 ( click the link to review the publication )

Chem Res Toxicol, 2018, 31(2):127-136

PubMed: 29156121 ( click the link to review the publication )

Sci Adv, 2018, 4(8):eaas9784

PubMed: 30101190 ( click the link to review the publication )

Nat Cell Biol, 2017, 19(10):1274-1285

PubMed: 28892080 ( click the link to review the publication )

Elife, 2017, 6e26733

PubMed: 28737489 ( click the link to review the publication )

J Neurochem, 2017, 143(3):282-293

PubMed: 28902407 ( click the link to review the publication )

Int J Cardiol, 2017, 227:201-208

PubMed: 27839819 ( click the link to review the publication )

Am J Physiol Renal Physiol, 2017, 313(2):F404-F413

PubMed: 28381458 ( click the link to review the publication )
PLoS One, 2017, 12(10):e0187123

PubMed: 29073235 ( click the link to review the publication )

J Mol Neurosci, 2017, 61(2):267-278

PubMed: 28054226 ( click the link to review the publication )

Pharm Res, 2016, 33(2):395-403

PubMed: 26415646 ( click the link to review the publication )

Int J Mol Med, 2016, 37(3):631-8

PubMed: 26846204 ( click the link to review the publication )

J Virol Methods, 2016, 238:13-20

PubMed: 27678028 ( click the link to review the publication )

Oncotarget, 2016, 7(6):6711-26

PubMed: 26744319 ( click the link to review the publication )

Oncogene, 2015, 10.1038/onc.2015.32

PubMed: 25728673 ( click the link to review the publication )

Antiviral Res, 2015, 120:140-6

PubMed: 26086883 ( click the link to review the publication )

Drug Metab Dispos, 2014, 42(3):318-22

PubMed: 24335466 ( click the link to review the publication )

Arch Environ Con Tox, 2014, 68(1):31-7

PubMed: 25194241 ( click the link to review the publication )

Biochim Biophys Acta, 2013, 1833(3):652-62

PubMed: 23228564 ( click the link to review the publication )

Drug Metab Dispos, 2013, 42(3):318-22

PubMed: 24335466 ( click the link to review the publication )

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述

靶点

calcineurin ^[1]
(Cell-free assay)

7 nM

体外研究

在T细胞中，Cyclosporin A与亲环蛋白(免疫亲和素)结合^[1]，形成亲环蛋白-Cyclosporin A复合体，其结合并抑制钙调磷酸酶。^[2]Cyclosporin A 抑制钙调磷酸酶，IC50 是7 nM^[3]，随后阻断NF-AT核转运。 ^[4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体，IC50 是39 nM。^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
rat H42E cells	M3jVbmN6fG:2b4jpZ:Kh[XO\|YYm=			MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiSESySUBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IFHUVEBt\X[nbDygWGM2OD12MjFOwG0>	NHr4c\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWwNlA2QCd-MUm1NFIxPTh:L3G+
mouse L929 cells	NEHwWWpEgXSxdH;4bYPDqGG\|c3H5		NFfNdXY{KGSjeYO=	NH;obHFEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNQTJ7IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFNiYYPzZZktKEmFNUC9N\|Ah\|ryP	NYjCcHBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFUyQTVpPkK1PVg2OTl3PD;hQi=>
human Huh-Mono cells	MkTQR5l1d3SxeHnjxsBie3OjeR?=		MljpN{Bl[Xm\|	NFXI[GtEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvTX;uc{Bk\WyuczDh[pRmeiB\|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;MkSg{txO	M{XyRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O\|Y3Lz5zOE[yOVc3PjxxYU6=
MDCK cells	NH;GdXNEgXSxdH;4bYPDqGG\|c3H5			NVu4UG41S3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczygWGM2OD17Lkmg{txO	M1jYZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
human LCC-6 cells	NYrKNoNwS3m2b4TvfIlkyqCjc4PhfS=>		NV61VWZ1PzJiaB?=	MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMR2MuPiClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:QC5\|IN88US=>	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ\|MkC0NFI9N2F-
human HeLa cells	MXnDfZRwfG:6aXRCpIF{e2G7			MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzMEBVSzVyPUiuN{DPxE1?	NH3NWVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
african green monkey Vero cells	MlrmSpVv[3Srb36gZZN{[Xl?		MljaNlQhcA>?	NVfHb\|dDSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhX2W\|dDDObYxmKH[rcoXzJIlv\mWldHXkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IG\ldo8h[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5idnnyZYwhWk6DIIP5cpRp\XOrczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBKSzVyPUig{txO	NVfDOZk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVEzQDZpPkG5OFUyOjh4PD;hQi=>
human Huh-9-13 cells	M3nsT2N6fG:2b4jpZ:Kh[XO\|YYm=		Moq2N{Bl[Xm\|	NYHn[lBOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqLUmtNVMh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3Ojef-8kEBESzVyPUeg{txO	NVrjNokyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
human HuH6 cells	MVrDfZRwfG:6aXRCpIF{e2G7		MnT2N{Bl[Xm\|	NXe4eWZ4S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNjDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCFQ{WwQVch\|ryP	NHPyUYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human Huh5-2 cells	MX\DfZRwfG:6aXRCpIF{e2G7		NHrBUok{KGSjeYO=	MXzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYg2NTJiY3XscJMh[W[2ZYKgN{Bl[Xm\|IHL5JG1VXCCjc4PhfUAtKEOFNUC9OkDPxE1?	NYr1[3BuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
human HuH7 cells	M2K3VGN6fG:2b4jpZ:Kh[XO\|YYm=			NGfQTGJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILlcIVie2Vib3[gcIFkfGG2ZTDk[Yh6\HKxZ3XuZZNmNCCLQ{WwQVUvQDFizszN	NX;xboJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC5OFM{QTBpPkKwPVQ{OzlyPD;hQi=>
human 2008 cells	NGHUOZlHfW6ldHnvckBie3OjeR?=			NF;peZNKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEGgbY4hcHWvYX6gNlAxQCClZXzsd{whUUN3ME20Mlc5KM7:TR?=	Mn[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5MEe4PFQoRjF6N{C3PFg1RC:jPh?=
human HeLa cells	NXrPfpU5TnWwY4Tpc44h[XO\|YYm=			NVnYVJluSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUG4JIlv\mWldHXkJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXQxvJygWGM2OD12Lk[g{txO	M4HtR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
leukemia CEM cells	MlXkSpVv[3Srb36gZZN{[Xl?			Mk\oTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIF3EVlEhWC2JbInjc5Bzd3SnaX6gRWJEKFS{YX7zdI9zfGW{IIXzbY5oKGyndXvlcYliKEOHTTDj[YxteyxiSVO1NF0{NjRizszN	NHTMWXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkO2NVM5Pyd-MUKzOlE{QDd:L3G+
human Caco2 cells	M2PyU2Z2dmO2aX;uJIF{e2G7			MU\ubIljcXSrb36gc4YhWC2pcDDpckBpfW2jbjDDZYNwOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[Ilod3irbjDl[oZtfXhuIFnDOVA:Oi57IN88US=>	M1HybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{iyOFU5Lz5{M{O4NlQ2QDxxYU6=
human MDA435/LCC6MDR cells	NH7DcFdEgXSxdH;4bYPDqGG\|c3H5		MWG3Nog>	MnzUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR\|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=>	NH7DfFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNFQxOid-MkKzNlA1ODJ:L3G+
guinea pig ventricular myocytes	NVvyXotyTnWwY4Tpc44h[XO\|YYm=			NUS4V\|Q{UW6qaXLpeIlwdiCxZjDMMZR6eGViY3HsZ4l2dSClaHHucoVtKG2nYYP1doVlKHW\|aX7nJJdpd2ynLXPlcIwheGG2Y3igZ4xidXBiaX6g[5VqdmWjIIDp[{B3\W62cnnjeYxieiCveX;jfZRmeyxiSVO1NF0zKM7:TR?=	M1y3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{[xNFAxLz5{Mke2NVAxODxxYU6=
KB/MDR cell	NYDGNppOTnWwY4Tpc44h[XO\|YYm=		M1HwSlczKGh?	NEi0ZoNKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhU0JxTVTSJINmdGxibHnu[UBi\nSncjC3NkBpeiCxZjDkdpVoKGW6cH;zeZJmNCCLQ{WwQVEvPSEQvF2=	M4HHNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
A2780/ADR cells	NXK0NodlTnWwY4Tpc44h[XO\|YYm=			M1vsUmlvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4h\XiycnXzd4VlKGmwIFGyO\|gxN0GGUjDj[YxteyCkeTDjZYxk\WmwIFHNJIF{e2G778{MJGlEPTB;MT60NVI2PCEQvF2=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7MEC5OEc,OTd6OUCwPVQ9N2F-
human K562/R7 cells	NVKySGI4TnWwY4Tpc44h[XO\|YYm=	MXSxJO69VQ>?	NFvqUoU4OiCq	MnXVVI91\W62aXH0bY9vKG:oIHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGGpYXnud5Qh\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKEt3NkKvVlch[2WubIOgZZN{\XO\|ZXSgZZMh\G:6b4L1ZolkcW5iSVO1NEBifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD63JO69VQ>?	NGfmN3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[zOFA1OSd-MkW2N\|QxPDF:L3G+
LLC-PK1 epithelial cells	Mk\ZSpVv[3Srb36gZZN{[Xl?			NFryW3BKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNCCvb4Xz[UBNNW2mckHiJIV5eHKnc4Pl[EBqdiCOTFOtVGsyKGWyaYTo[Yxq[WxiY3XscJMhfXOrbnegZ4Ft[2Wrbj3BUUBxd2yjcnnzZZRqd25iYYPzZZnwxIxiSVO1NF0xNjdizszN	NWT2e\|lMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
mouse L5178 cells	NHzGOpVHfW6ldHnvckBie3OjeR?=		M3f6dlIxKG2rboO=	MWPJcohq[mm2aX;uJI9nKGi3bXHuJGFDS0JzLX3l[IlifGWmIILoc4RidWmwZTCxNlMh\W[obIX4JIlvKG2xdYPlJGw2OTd6IHPlcIx{KGW6cILld5NqdmdiaIXtZY4hVUSUMTDh[pRmeiB{MDDtbY5{KGK7IF\BR3Mh[W6jbInzbZMtKEmFNUC9NE43PSEQvF2=	Mn\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|NEG3OFUoRjJzM{SxO\|Q2RC:jPh?=
KB cell	MkXwSpVv[3Srb36gZZN{[Xl?			NXT2UHVjUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEIHPlcIwhdGmwZTDh[pRmeiB5MjDoJI9nKGS{dXeg[Zhxd3O3cnWsJGlEPTB;MD62JO69VQ>?	NH\0SZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS4NVk6OSd-MUW0PFE6QTF:L3G+
mouse mammary carcinoma cell line EMT6/AR 1.0	NUeyNnlTTnWwY4Tpc44h[XO\|YYm=			Mom1VoV3\XK\|YXyg[YZn\WO2IH;uJJRp\SCjY3P1cZVt[XSrb36gc4YhYzOKXT2g[IF2dm:{dXLpZ4lvKGmwIH3veZNmKG2jbX3hdpkh[2G{Y3nuc41iKGOnbHygcIlv\SCHTWS2M2FTKDFwMDygTWM2OD1yLkS0JO69VQ>?	NWjq[\|duRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwPVg3PzFpPkGwNFk5PjdzPD;hQi=>
CK1 cells	M{nuWWZ2dmO2aX;uJIF{e2G7			NVzPVmZQSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUOzJIlv\mWldHXkJIlvKEONMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFch\GG7czygWGM2OD1yLkOg{txO	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
HEK293 cells	MlnDSpVv[3Srb36gZZN{[Xl?			NW\n[Jg6UW6qaXLpeIlwdiCxZjDPRXRROUJzIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDwdo91\WmwLX3l[IlifGWmIIDpeIF3[XO2YYTpckB2eHSja3ZvwKwhU2l;MD6yJO69VQ>?	NF;KV4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4O\|k5Pid-MkK1PFc6QDZ:L3G+
human MDA435/LCC6MDR cells	M3fS[mZ2dmO2aX;uJIF{e2G7		NXfaVXZJPSCmYYnz	NYjYW4ZzVW:mdXzheIlwdiCxZjDQMYdxKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gbJVu[W5iTVTBOFM2N0yFQ{\NSHIh[2WubIOgZZN{\XO\|ZXSgZZMhemW4ZYLzbY5oKHCjY3zpeIF5\WxicnXzbZN1[W6lZTDt[YF{fXKnZDDhd{Bk\WyuIIP1dpZqfmGuIHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XluIFXDOVA:OC5yM{Kg{txONg>?	NEnIRlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4OVE6PSd-MkW5PFUyQTV:L3G+
human PBMC	NFTiTI9Rem:uaX\ldoF1cW:wIHHzd4F6			Ml3IRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCSQl3DMEBKSzVyPUGyJI5O	M3\jOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3Nke5N\|A6Lz5zNU[3PVMxQTxxYU6=
mouse T cells	MYfGeY5kfGmxbjDhd5NigQ>?		NGe2[WM1KGSjeYO=	NH75[lBKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCvb4Xz[UBVKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geJdwKHejeTDtbZhm\CCueX3wbI9kgXSnIILlZYN1cW:wIHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[XluIHnjOVA:OTFibl2=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6NEm4O{c,OjJ7OES5PFc9N2F-
human splenocytes	MW\GeY5kfGmxbjDhd5NigQ>?		MX:0JIRigXN?	MmH5TY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hcHWvYX6gd5Bt\W6xY4n0[ZMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifdHj5cYllcW6nIHnuZ49zeG:{YYTpc44h[W[2ZYKgOEBl[Xm\|IHL5JIFtdG:pZX7pZ{BucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJIF{e2G7LDDJR\|UxRTFyIH7N	NUHYV4lIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlc5OzFpPkG5PFI4QDNzPD;hQi=>
human T-cell	NUTWSJF{TnWwY4Tpc44h[XO\|YYm=			NVPVZWZtUW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJHQu[2WubDDwdo9lfWO2aX;uJI9nKGy7bYDoc4tqdmViSVytNkwhUUN3ME24MlEhdk1?	Mof2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzV\|N{OzNUc,PzV\|N{OzNVww[T5?
human Jurkat cells	MXLGeY5kfGmxbjDhd5NigQ>?		NX7NS4N4OSCq	MnfXRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDKeZJs[XRiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidnSrLVPEN{9ETDJ6IHHueIljd2S7LXnu[JVk\WRiSVytNkBxem:mdXP0bY9vKHS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJIFvfGlvQ1SzM2NFOjhiYX70bYJw\HliY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJIJ6KGmvbYXuc5N2eHC{ZYPzbY9vKGG\|c3H5MEBGSzVyPUWgcm0>	NHL0UWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzN\|c6PSd-MUm5N\|M4QTV:L3G+
human PBMC	NIjtVJFHfW6ldHnvckBie3OjeR?=		MWixJIg>	MYfBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIGDCUWMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidnSrLVPEN{9ETDJ6IHHueIljd2S7LXnu[JVk\WRiSVytNkBxem:mdXP0bY9vKHS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJIFvfGlvQ1SzM2NFOjhiYX70bYJw\HliY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJIJ6KGmvbYXuc5N2eHC{ZYPzbY9vKGG\|c3H5MEBGSzVyPUOuNUBvVQ>?	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl\|M{e5OUc,OTl7M{O3PVU9N2F-
Jurkat cells	M3i0dWZ2dmO2aX;uJIF{e2G7			NXf5eYR{UW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhf2G\|IH3lZZN2emWmIHL5JIlvcGmkaYTpc44hd2ZidHjlJGlNNTJicILv[JVkfGmxbjDpckBLfXKtYYSgZ4VtdHNuIFnDOVA:OiCwTR?=	NYnFRWhSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS2OFM{OzdpPkG0OlQ{OzN5PD;hQi=>
HepG2 cells	MUnGeY5kfGmxbjDhd5NigQ>?			M{LWPGlvcGmkaYTpc44hd2ZibHn2[ZIhe3SjZ3WgVIxie22xZHn1cUBj\XKpaHXpJIlv\mWldHnvckBqdiCKZYDHNkBk\WyuczygTWM2OD1zLk[1JI5O	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6NkGyOEc,OjJ3OE[xNlQ9N2F-
Jurkat	NVTYfIJDTnWwY4Tpc44h[XO\|YYm=			MkHGWIhmKGOxbYDveY5lKHejczD0[ZN1\WRiaX6geol1em9iZn;yJIlvcGmkaYTpc44hd2ZiSVytNkBz\WynYYPlJJN1cW23bHH0[YQhf2m2aDDQUWEwcW:wb335Z4lvKGK7IFr1dotifCClZXzsd{4tKEmFNUCgQUAxNjBzMjFOwG0v	MkfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzR5M{W0N{c,PzR5M{W0N\|ww[T5?
mouse spleen cells	NVzWd21pTnWwY4Tpc44h[XO\|YYm=			MonlWIhmKGOxbYDveY5lKHejczD0[ZN1\WRiaX6geol1em9iZn;yJIlvcGmkaYTpc44hd2ZicILvcIln\XKjdHnvckBqdiCERF[xJI1wfXOnIIPwcIVmdiClZXzsd{B{fGmvdXzheIVlKHerdHigZ49v[2GwYY\hcIlvKEFiKFPvcmEqNixiSVO1NEA:KDBwMESg{txONg>?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84PDd\|NUSzK\|44PDd\|NUSzQE9iRg>?
Sf9	NFXoN3BHfW6ldHnvckBie3OjeR?=			NFTVfZBqdmirYnn0bY9vKG:oIG\ldoFx[W2rbDDzeIlufWyjdHXkJGFVWCCqeXTyc4x6e2m\|IHnuJI1mdWK{YX7ld{Bnem:vIF3EVlEu\XiycnXzd4lv\yCVZkmgZ4VtdHNuIFvpJF0hOC5yMjFOwG0v	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84PTF2Mk[zK\|44PTF2Mk[zQE9iRg>?
LLC-PK1	Ml\VSpVv[3Srb36gZZN{[Xl?			MVz0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIHnuJG1FWjFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNbTC9JFgvPCEQvF2u	Mnj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzZ6MUC1PUc,PzZ6MUC1PVww[T5?
HeLa	M2PzSGZ2dmO2aX;uJIF{e2G7			MkTrbY5pcWKrdHnvckBw\iCOVFO0JJVxfGGtZTDpckBu\W2kcnHu[UB3\XOrY3zlJIZzd21iTWLQNU1mgHC{ZYPzbY5oKEinTHGgZ4VtdHNuIFvpJF0hPSEQvF2u	NXrGRlVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{m2NVcxPid-N{m2NVcxPjxxYU6=
Caco-2	MV3GeY5kfGmxbjDhd5NigQ>?			MXH0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIHnuJGNi[29vMjDj[YxteyxiS32gQUA{NjhizszNMi=>	MkG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDh2MkS1Nkc,QDh2MkS1Nlww[T5?
spleen	Mo\hSpVv[3Srb36gZZN{[Xl?			MXXF[oZm[3Rib3[gZ49ueG:3bnSgc44hUUxvMjDwdo9lfWO2aX;uJIZzd21iYXzsc4FvfGmpZX6td5RqdXWuYYTl[EBud3W\|ZTDzdIxm\W5iY3XscJMtKEmFNUCgQUAxNjBzIN88UU4>	NVezc3UxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEi5N\|g{QSd-OEi5N\|g{QTxxYU6=
COS	Mk\rSpVv[3Srb36gZZN{[Xl?			NGS1XFJqdmirYnn0bY9vKG:oIGTheZJw[2ixbHH0[UB2eHSja3WgbY4hSVOEVD3lfJBz\XO\|aX7nJGNQWyClZXzsd{whU2liPTCyOEDPxE1w	NV[xcVlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUS1PFc5PSd-OUS1PFc5PTxxYU6=
LLC-PK1	NWLyb5ZWTnWwY4Tpc44h[XO\|YYm=			NX\YUpk5cW6qaXLpeIlwdiCxZjDEc5hwenWkaXPpckB1emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JEhj[XOjbDD0c{BieGmlYXypJEhFcWexeHnuPkAxNjhidV2pJIlvKE2GUkGt[ZhxemW\|c3nu[{BNVENvUFuxJINmdGy\|LDDJR\|UxKD1iMz62OkDPxE1w	NUTNSmFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUmxOFc6Oid-OUmxOFc6OjxxYU6=
CEM/VLB100	M2HrXGZ2dmO2aX;uJIF{e2G7			M{i2UIlvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gR2VON1[OQkGwNEBk\WyuczygT4khRSBzLkig{txONg>?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODF7OEKyO{c,OTBzOUiyNlc9N2F-
HEK	M2j3W2Z2dmO2aX;uJIF{e2G7			M{nmXYlvcGmkaYTpc44hd2ZiQl3HJJVxfGGtZTDpckBu\W2kcnHu[UB3\XOrY3zld{Bnem:vIF3ydFIu\XiycnXzd4lv\yCKRVugZ4VtdHNuIFvpJF0hOTBizszNMi=>	NXXTT2tORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0NlE3PThpPkGwOFIyPjV6PD;hQi=>
HEK	Mn[3SpVv[3Srb36gZZN{[Xl?			MV3pcohq[mm2aX;uJI9nKEKPRzD1dJRic2ViaX6gcYVu[nKjbnWgeoV{cWOuZYOg[pJwdSCPUmCyMYV5eHKnc4PpcochUEWNIHPlcIx{NCCNaTC9JFIyKM7:TT6=	Mli1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB2MkG2OVgoRjFyNEKxOlU5RC:jPh?=
HeLa	NFLmSVdHfW6ldHnvckBie3OjeR?=			NHjGWmxqdmirYnn0bY9vKG:oIGTheZJw[2ixbHH0[UB2eHSja3WgbY4hVlSFUD3lfJBz\XO\|aX7nJGhmVGFiY3XscJMtKEmFNUCgQUAyKM7:TT6=	MnjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB3NkW4OFMoRjFyNU[1PFQ{RC:jPh?=
LLC-PK1	MmDFSpVv[3Srb36gZZN{[Xl?			MV\pcohq[mm2aX;uJI9nKEyWQ{SgeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHXzJIZzd21iTWLQNk1mgHC{ZYPzbY5oKEyOQzDQT\|Eh[2WubIOsJGtqKD1iND63JO69VS5?	MlroQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB3N{CwOFkoRjFyNUewNFQ6RC:jPh?=
Sf9	NH;yVZNHfW6ldHnvckBie3OjeR?=			NYrxeIRncW6qaXLpeIlwdiCxZjDUZZVzd2Oqb3zheIUhfXC2YXvlJIlvKG2nbXLyZY5mKH[nc3njcIV{KG[{b32gRpNmeC2neIDy[ZN{cW6pIGPmPUBk\WyuczygT4khRSByLkOg{txONg>?	NVLzOoJMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2OFg1PzBpPkGwOlQ5PDdyPD;hQi=>
Caco-2	M1TBcGZ2dmO2aX;uJIF{e2G7			NV7YWXZicW6qaXLpeIlwdiCxZjDEbYdwgGmwIITyZY5{\XCrdHjlcIlidCC2cnHud5BwenRiKHLhd4FtKHSxIHHwbYNidCliKFTp[495cW58IEWgeW0qKGmwIFPhZ48uOiClZXzsd{whUUN3MDC9JFEvOyEQvF2u	NWHlcFZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4NlAyOzdpPkGwPFIxOTN5PD;hQi=>
CEM/VLB100	NY\KUnd1TnWwY4Tpc44h[XO\|YYm=			NXrqS2ZqcW6qaXLpeIlwdiCxZjDDZYxk\WmwLVHNJIVn\my3eDCoR4Ft[2Wrbj3BUVohOC5{NTD1UUkhcW5iQ1XNM3ZNSjFyMDDj[YxteyxiSVO1NEA:KDNwNEGg{txONg>?	NEKybHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEi5NVEyPCd-MUC4PVEyOTR:L3G+
Sf9	NYLl[ZRITnWwY4Tpc44h[XO\|YYm=			NHX3cHBqdmirYnn0bY9vKG:oIFHUVIF{\SCrbjDt[Y1jemGwZTD2[ZNq[2ynIH\yc40hSkOUUD3lfJBz\XO\|aX7nJHNnQSClZXzsd{whU2liPTCwMlUh\|ryPLh?=	NHzw[4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUSzO\|M5OCd-MUG0N\|c{QDB:L3G+
NIH-3T3-G185	MYLGeY5kfGmxbjDhd5NigQ>?			NFPQfWZqdmirYnn0bY9vKG:oIFTheY5wenWkaXPpckBm\m[udYigbY4hVkmKLUPUN{1IOTh3IHPlcIx{NCCLQ{WwJF0hOS52IN88UU4>	NUnacIluRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NVY2OTRpPkGxO\|E3PTF2PD;hQi=>
NIH-3T3-G185	NUDMOVlqTnWwY4Tpc44h[XO\|YYm=			M372eIlvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gUmlJNTOWMz3HNVg2KGOnbHzzMEBKSzVyIE2gNU44KM7:TT6=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	MnvUSpVv[3Srb36gZZN{[Xl?			NV;EU\|ZvcW6qaXLpeIlwdiCxZjDSbI9l[W2rbnWgNVI{KGWoZnz1fEBqdiCQSVitN3Q{NUdzOEWgZ4VtdHNuIFnDOVAhRSBzLkeg{txONg>?	M4LM[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{G2OVE1Lz5zMUexOlUyPDxxYU6=
NIH-3T3-G185	MVHGeY5kfGmxbjDhd5NigQ>?			NFnHUGJqdmirYnn0bY9vKG:oIFzEV{04PTFiZX\mcJV5KGmwIF7JTE0{XDNvR{G4OUBk\WyuczygTWM2OCB;IEGuPEDPxE1w	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	MnO0SpVv[3Srb36gZZN{[Xl?			NIXSSVJqdmirYnn0bY9vKG:oIGTleJJidWW2aInsdo9{[W2rbnWg[YZndHW6IHnuJG5KUC1\|VEOtS\|E5PSClZXzsd{whUUN3MDC9JFIvOSEQvF2u	MkTYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	NH3jdVJHfW6ldHnvckBie3OjeR?=			MWLpcohq[mm2aX;uJI9nKE[udX:tN{1CVSCnZn\seZghcW5iTlnIMVNVOy2JMUi1JINmdGy\|LDDJR\|UxKD1iMj60JO69VS5?	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	NYfrNopuTnWwY4Tpc44h[XO\|YYm=			M1zFS4lvcGmkaYTpc44hd2ZiSlOtNUBm\m[udYigbY4hVkmKLUPUN{1IOTh3IHPlcIx{NCCLQ{WwJF0hPC5\|IN88UU4>	MoqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
HCT15	NXnUT3Q1TnWwY4Tpc44h[XO\|YYm=	NFnCV3IxNjRidH:gOk4{KHWpL33M		NYD3VmRRVW:mdXzheIlwdiCxZjDQMYdxKGmwIHj1cYFvKEiFVEG1JINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKESLTlnCMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGG2IECuOEB1dyB4LkOgeYcwdUxw	NUfjTWFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3OVQ3ODJpPkGxO\|U1PjB{PD;hQi=>
UO31	NFjERlJHfW6ldHnvckBie3OjeR?=	Mn\HNE41KHSxIE[uN{B2\y:vTB?=		Mn3wUY9lfWyjdHnvckBw\iCSLXfwJIlvKGi3bXHuJHVQOzFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gSGlPUUJvaX7keYNm\CCleYTveI95cWOrdImgZZQhOC52IITvJFYvOyC3Zz;tUE4>	NX\K[YVTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3OVQ3ODJpPkGxO\|U1PjB{PD;hQi=>
CAKI1	M4TlW2Z2dmO2aX;uJIF{e2G7	MYiwMlQhfG9iNj6zJJVoN22O		MkHjUY9lfWyjdHnvckBw\iCSLXfwJIlvKGi3bXHuJGNCU0lzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJGRKVkmELXnu[JVk\WRiY4n0c5RwgGmlaYT5JIF1KDBwNDD0c{A3NjNidXevcWwv	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd3NE[wNkc,OTF5NUS2NFI9N2F-
MDA-435/LCC6 and MDA-435/LCC6-MDRI	M1P0NWZ2dmO2aX;uJIF{e2G7			M33FS2NwdmOnboTyZZRqd25idHjheEBz\WS3Y3XzJJRp\SCmaX\m[ZJmdmOnIHnuJJJmfmW{c3HsJI9nKESRWDDhZ4N2dXWuYYTpc44h[mW2d3XlckBOTEFvNEO1M2xESzZiYX7kJG1FSS12M{WvUGNEPi2PRGLJJINmdGy\|IHL5JFUxLS5uIFXDOVAhRSByLkWg{txONg>?	MnT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6	NVXz[ppvTnWwY4Tpc44h[XO\|YYm=			NYLRfJR3TW[oZXP0JI9vKHKndnXyd4FtKG:oIGuzTH0uKF[ETDDhZ4N2dXWuYYTpc44hcW5iTVTBMVQ{PS:OQ1O2JEhR\3BvbnXnZZRqfmVrIHPlcIx{NixiRVO1NEA:KDBwNjFOwG0v	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
MDA-435/LCC6 and MDA-435/LCC6-MDRI	M4TDS2Z2dmO2aX;uJIF{e2G7			NXvDUZByS2:wY3XueJJifGmxbjD0bIF1KHKnZIXj[ZMh\GmoZnXy[Y5k\SCrbjDy[ZZmenOjbDDv[kBcO0ifVlLMJIFk[3WvdXzheIlwdiCkZYT3[YVvKE2GQT20N\|UwVEOFNjDhcoQhVUSDLUSzOU9NS0N4LV3EVmkh[2WubIOgZpkhPTBnLDDFR\|UxKD1iMD62JO69VS5?	NF7HcXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
MDA435/LCC6 MDR1	MmrvSpVv[3Srb36gZZN{[Xl?			M2juO5JmfmW{c3HsJI9nKF[rbnLsZZN1cW6nIHHjZ5VufWyjdHnvckApXmmwYnzhd5RqdmV8IECuNFA2KHWPKTDpckBOTEF2M{WvUGNEPiCPRGKxJINmdGy\|LDDFR\|UxKD1iMD62JO69VS5?	NFi5R\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
MDA-435/LCC6 (Pgp-negative)	M3vsRmZ2dmO2aX;uJIF{e2G7			NEXtOHdG\m[nY4Sgc44hemW4ZYLzZYwhd2ZiRF;YJIFk[3WvdXzheIlwdiCrbjDNSGEuPDN3L1zDR\|YhMFCpcD3u[YdifGm4ZTmgZ4VtdHNwLDDFR\|UxKD1iMD63JO69VS5?	NGXIT4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
MDA-435/LCC6	M1riVWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7			NYHxdVRlT3Kxd4ToJIlvcGmkaYTvdpkh[WO2aY\peJkhd25iTVTBMVQ{PS:OQ1O2JEhR\3BvcH;zbZRqfmVrIHj1cYFvKGK{ZXHzeEBk[W6lZYKgZ4VtdHNwLDDJR\|UxKD1iMTFOwG0v	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
MDA-435/LCC6-MDRI	M3yzXmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7			MYnHdo94fGhiaX7obYJqfG:{eTDhZ5Rqfmm2eTDvckBOTEFvNEO1M2xESzZvTVTSTUApWGeyLX7l[4F1cX[nKTDoeY1idiCkcnXhd5Qh[2GwY3XyJINmdGy\|LjygTWM2OCB;IEGuNUDPxE1w	NIDWU5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
BTI-TN5B1-4	MoewSpVv[3Srb36gZZN{[Xl?			NIi0cIRCXFBiaInkdo9tgXOrczDpckBu\W2kcnHu[UBnemGldHnvckBnem:vIFjp[4ghTmm4ZTCoRnRKNVSQNVKxMVQqKGOnbHzzMEBMdSB;IECuNVch\|ryPLh?=	M{Xye\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i1Olg1Lz5zMUe4OVY5PDxxYU6=
Jurkat	MVLGeY5kfGmxbjDhd5NigQ>?			MnO1TY5pcWKrdHnvckBw\iCycn;keYN1cW:wIH;mJGlNNTJic3XjdoV1cW:wIHnuJGp2emujdDDj[YxteyxiSVO1NEA:KDBwMEC3JO69VS5?	MkXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
Jurkat	M17ocWZ2dmO2aX;uJIF{e2G7			M3\v[2lvcGmkaYTpc44hd2ZicILv[JVkfGmxbjDv[kBj\XSjLXfhcIFkfG:\|aXThd4UhUUxvMjDSS2EhcW5iSoXyb4F1KGOnbHzzMEBKSzVyIE2gNE4xODhizszNMi=>	MoXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
LLC-PK1	NHGxV\|dHfW6ldHnvckBie3OjeR?=			NIfVS5hqdmO{ZXHz[UBqdiCYaX7icIF{fGmwZTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUAyNjNizszNMi=>	M3\jT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[xNVE{Lz5zMUm2NVEyOzxxYU6=
LLC-PK1	M1;3OmZ2dmO2aX;uJIF{e2G7			MWLpcoNz\WG\|ZTDpckBE[WylZXnuMWFOKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKGmwIF3EVlEu\XiycnXzd4lv\yCOTFOtVGsyKGOnbHzzMEBMcSB;IESuOlYh\|ryPLh?=	M2Pw[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[xNVE{Lz5zMUm2NVEyOzxxYU6=
MDCK	MlrjSpVv[3Srb36gZZN{[Xl?			NFnOcotqdmirYnn0bY9vKG:oIFTp[495cW5idILhcpNmeGm2aHXsbYFtKHS{YX7zdI9zfCBqYnHzZYwhfG9iYYDpZ4FtMSCrbjDNSHIyNWW6cILld5NqdmdiTVTDT{Bk\WyuczygT4khRSB{LkG4JO69VS5?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjF\|NEm0OUc,OTJzM{S5OFU9N2F-
MDCK	NV\MNpBpTnWwY4Tpc44h[XO\|YYm=			M3\mfolvcGmkaYTpc44hd2ZiVnnuZoxie3SrbnWgeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTDpckBOWlB{LXX4dJJme3OrbnegUWREUyClZXzsd{whU2liPTC4MlEyKM7:TT6=	Mk\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzM{S5OFYoRjF{MUO0PVQ3RC:jPh?=
LLC-PK1	NU\sUVNGTnWwY4Tpc44h[XO\|YYm=			Ml7WbY5kemWjc3WgbY4hS2GuY3Xpck1CVSCrboTyZYNmdGy3bHHyJIFk[3WvdXzheIlwdiCrbjDt[JIy[i2neIDy[ZN{cW6pIFzMR{1RUzFiY3XscJMtKEurIE2gN{42KM7:TT6=	M2jF[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MkO1NlY4Lz5zMkKzOVI3PzxxYU6=
LLC-PK1	MVfGeY5kfGmxbjDhd5NigQ>?			NV3VbI44cW6lcnXhd4UhcW5iQ3HsZ4Vqdi2DTTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBu\HJzYT3lfJBz\XO\|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOy54NTFOwG0v	NY\aNHlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN\|UzPjdpPkGyNlM2OjZ5PD;hQi=>
HL-60	NIjCfYZHfW6ldHnvckBie3OjeR?=			MWfJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iZn;ycZlteGWydHnk[UBz\WOncITvdkApTlCUKTDv[kBpfW2jbjDs[ZVs\W2rYTDIUE03OCClZXzsd{whUUN{MDC9JFMvPiEQvF2u	Ml;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ\|NkGzPFgoRjF{M{[xN\|g5RC:jPh?=
High Five cells	NIj0bIxHfW6ldHnvckBie3OjeR?=			Ml:2bY5pcWKrdHnvckBw\iCWYYXyc4Np\W6xZHXvfJlkcG:uYYTlJJVxfGGtZTjDd2EhOzC3TTygN\|AhLSCxZjDjc451em:uKTDpckBu\W2kcnHu[UB3\XOrY3zld{BxemWyYYLl[EBnem:vIFjp[4ghTmm4ZTDj[YxteyCrbn\lZ5Rm\CC5aYToJJRp\SCDQlPCNVEh[mGldXzveolzfXNuIFvpJF0hQS53IN88UU4>	M2\BV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NEC0NlM6Lz5zMkSwOFI{QTxxYU6=
MDCKII	MULGeY5kfGmxbjDhd5NigQ>?			NFTiWoRqdmirYnn0bY9vKG:oIFPldol3[XO2YYTpckB2eHSja3WgbY4hV0GWUEKt[ZhxemW\|c3nu[{BOTEONSVmgZ4VtdHNuIFvpJF0hOC5{M{ig{txONg>?	Mn61QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ3M{i4NVMoRjF{NUO4PFE{RC:jPh?=
Caco-2	MkTHSpVv[3Srb36gZZN{[Xl?			MlfOeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDDv[kBlcWexeHnuJEhj[XOjbDD0c{BieGmlYXypJIlvKEOjY3:tNkBk\WyuczygT4khRSByLkO1JO69VS5?	NVr2TII2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2N\|YyPTNpPkGyOlM3OTV\|PD;hQi=>
SK-E2	NXnWSmdVTnWwY4Tpc44h[XO\|YYm=			NELGU45qdmO{ZXHz[UBqdiCkb3TpdJkhcW62cnHj[YxtfWyjcjDhZ4N2dXWuYYTpc44hMEKxZHnwfVohOC5{IIXNLUBqdiCVSz3FNkBk\WyuczCo[ZhxemW\|c3nu[{BDW0WSKTygTWM2OCB;IEeuOUDPxE1w	M{DTO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O\|U6Lz5zMkezPVc2QTxxYU6=
SK-E2	M2XG[mZ2dmO2aX;uJIF{e2G7			MVzpcoNz\WG\|ZTDpckBlcWi7ZILv[ox2d3Knc3PlbY4hcW62cnHj[YxtfWyjcjDhZ4N2dXWuYYTpc44hMGSraInkdo9ndHWxcnXzZ4VqdjpiMTD1UUkhcW5iU1utSVIh[2WubIOgLIV5eHKnc4PpcochSlOHUDmsJGlEPTBiPTC3Mlgh\|ryPLh?=	M1ex[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O\|U6Lz5zMkezPVc2QTxxYU6=
HEK293	MnniSpVv[3Srb36gZZN{[Xl?			Ml;obY5pcWKrdHnvckBw\iCSaHHscI9q\GmwIIXweIFs\SBqUHjhcIxwcWSrbkqgNUB2VSliaX6gU2FVWC2FLXX4dJJme3OrbnegTGVMOjl\|IHPlcIx{NCCNaTC9JFAvOiEQvF2u	M3fSWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUOwPVA4Lz5zNEWzNFkxPzxxYU6=
T-cells	MYXGeY5kfGmxbjDhd5NigQ>?			MV\Jcohq[mm2aX;uJI9nKGGubH;n[Y5q[yClZXzsd{1{fGmvdXzheIVlKHC{b3zp[oVz[XSrb36gbY4hdW:3c3WgWEBk\WyuczDifUBucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJI1mfGixZDygTWM2OCB;IECuNFMzKM7:TT6=	NGCz[pk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEW5N\|E5Oid-MUS1PVMyQDJ:L3G+
T-cells	MXzGeY5kfGmxbjDhd5NigQ>?			MnjzTY5pcWKrdHnvckBw\iCjbHzv[4VvcWNiY3XscJMue3SrbYXsZZRm\CCycn;sbYZmemG2aX;uJIlvKGi3bXHuJHQh[2WubIOgZpkhdWm6ZXSgcJlueGixY4n0[UBz\WGldHnvckBu\XSqb3SsJGlEPTBiPTCwMlA4PCEQvF2u	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV7M{G4Nkc,OTR3OUOxPFI9N2F-
PMBC	NYfSbod5TnWwY4Tpc44h[XO\|YYm=		NYP0UmlpPzJiaILz	NXjWZ2JlTW[oZXP0JI9vKHC{b3zp[oVz[XSrb36gc4YhcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczCoVG1DSyliYYSgO\|IhcHJidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hQC57NzFOwG0v	M{HFPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MkCzNVY{Lz5zNUKwN\|E3OzxxYU6=
mouse spleen cells	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=			NYe1U\|Z7S3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJJNxdGWnbjDj[YxteyxiQ1O1NEA:KDFwM{Wg{txONg>?	M2Lse\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEixO\|YyLz5zN{C4NVc3OTxxYU6=
MDCK2	NHLp[lFHfW6ldHnvckBie3OjeR?=			M{X2S2lvcGmkaYTpc44hd2ZiaIXtZY4hVUSUMT3k[ZBmdmSnboSgZYNkfW23bHH0bY9vKG:oIHPhcINmcW5vQV2g[ZhxemW\|c3XkJIlvKE2GQ1uyJINmdGy\|LDDJR\|UxKD1iOT6zOEDPxE1w	M3\UflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[0N\|I4Lz5zN{[2OFMzPzxxYU6=
HEK293	NUjqWJBzTnWwY4Tpc44h[XO\|YYm=			NWHLSo5oUW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVEhVDZ3Uj;UUVYhVDN\|OVOvWG04KEZ5MkigcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW\|aX7nJFUxLSCrbnjpZol1cW:wIH;mJINzd3O\|IHzpcotqdmdiYomgbY1ufW6xYnzveEwhSWO2aY\peJkhRSByLkWg{txONg>?	NYHnRVlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ\|PD;hQi=>
HEK293	MVfGeY5kfGmxbjDhd5NigQ>?			Ml7RTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTU[gUFM{QUNxVF23JGY4OjiFIH31eIFvfCCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHTyeYchdGW4ZXygZ4F2e2mwZzC1NEUhcW6qaXLpeIlwdiCxZjDjdo9{eyCuaX7rbY5oKGK7IHntcZVvd2Kub4SsJGFkfGm4aYT5JF0hOC54IN88UU4>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293	M{DqOmZ2dmO2aX;uJIF{e2G7			NWXVOnlHUW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVNVOTl7Uj;UUVYhVDN\|OVOvWG04KEZ5MkjDJI12fGGwdDDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{A2OCViaX7obYJqfGmxbjDv[kBkem:\|czDsbY5scW6pIHL5JIludXWwb3Lsc5QtKEGldHn2bZR6KD1iMT6xJO69VS5?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293	MU\GeY5kfGmxbjDhd5NigQ>?			MnntTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTU[gSlM1O1JxVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDFzIN88UU4>	NWj3VHdvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ\|PD;hQi=>
HEK293	NFLibm5HfW6ldHnvckBie3OjeR?=			MkntTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTUWgTVMxPlJxVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDF3IN88UU4>	MknnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6NEi1OlMoRjF5OES4OVY{RC:jPh?=
MDCK2	MkTESpVv[3Srb36gZZN{[Xl?	MX2xNEB2VQ>?	MmfKOFAhdWmwcx?=	NGXaT25KdmirYnn0bY9vKG:oIHj1cYFvKHerbHSgeJlx\SCSZ4Cg[ZhxemW\|c3XkJIlvKE2GQ1uyJINmdGy\|IHHzd4V{e2WmIHHzJINidGOnaX6tRW0h[WOldX31cIF1cW:wIHH0JFExKHWPIHHmeIVzKDRyIH3pcpMv	NX\uUWpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5OlQyPzBpPkG3PVY1OTdyPD;hQi=>
A2780	M175eGZ2dmO2aX;uJIF{e2G7		NIrxfXo{OCCvaX7z	M3n2R2lvcGmkaYTpc44hd2ZiaIXtZY4hWGeyIHnuJGEzPzhyIHPlcIx{KGGodHXyJFMxKG2rboOgZpkhUG:nY3jzeEA{OzN2MjDhd5NigSxiSVO1NEA:KDFwNEGyOVQh\|ryPLh?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6M{CzOEc,OThyOEOwN\|Q9N2F-
A2780	NHHSZ5lHfW6ldHnvckBie3OjeR?=		MVKzNEBucW6\|	M1SwS2lvcGmkaYTpc44hd2ZiaIXtZY4hWGeyIHnuJGEzPzhyIHPlcIx{KGGodHXyJFMxKG2rboOgZpkh[2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFEvPDF{NUSg{txONg>?	MmTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyOEOwN\|QoRjF6MEizNFM1RC:jPh?=
Caco-2	NVvadFRwTnWwY4Tpc44h[XO\|YYm=			MWTJcohq[mm2aX;uJI9nKFBvZ3z5Z49xem:2ZXnuMY1m\GmjdHXkJHs{UF24aX7icIF{fGmwZTD0doFve3CxcoSgbY4hcHWvYX6gR4Fkdy1{IHPlcIx{NCCHQ{WwJF0hPDVwNTFOwG0v	NFj0e209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWyOFU6Oid-MUi1NlQ2QTJ:L3G+
A2780	MWPGeY5kfGmxbjDhd5NigQ>?			NYG1NGd1UW6qaXLpeIlwdiCxZjDQMYdxKGmwIHj1cYFvKGGmcnnhcZlkcW5vcnXzbZN1[W62IFGyO\|gxKGOnbHzzJIJ6KEixZXPod5QhOzN\|NEKgZZN{[XluIFnDOVAhRSBzLkSxNlU1KM7:TT6=	MlG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4N{i0PVUoRjF6Nke4OFk2RC:jPh?=
Huh-9-13	MlTKRY51cX[rcnHsJIF{e2G7		NUHwXm9tOyCmYYnz	NI\lN3pCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBDUUyQIEKwOlEuemW\|aYP0ZY51KEiFVjDn[Y5wfHmyZTCxZkBqdm[nY4Tl[EBqdiCqdX3hckBJfWhvOT2xN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyZYwhemWybHnjZZRqd25iYX\0[ZIhOyCmYYnzJIJ6KHG3YX70bZRifGm4ZTDSWE1RS1JuIFXDOVAhRSByLkCwOEDPxE1w	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTFyNECxN{c,OTlzMESwNVM9N2F-
Huh-9-13	M4O5eGFvfGm4aYLhcEBie3OjeR?=		NWPJU\|BHOyCmYYnz	MYDBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDjc41xd3WwZD3y[ZNqe3SjboSgTGNXKGenbn;0fZBmKDGkIHnu[oVkfGWmIHnuJIh2dWGwIFj1bE06NTF\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjCzJIRigXNiYomgdZVidnSrdHH0bZZmKFKWLWDDVkwhTUN3MDC9JFAvOzVizszNMi=>	MonxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	M4\WdWFvfGm4aYLhcEBie3OjeR?=		MnHqN{Bl[Xm\|	MVHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDCyK{1ENW2ndHj5cE0zLy2obIXvdo8u[3m2aXTpcoUuemW\|aYP0ZY51KEiFVjDn[Y5wfHmyZTCxZkBqdm[nY4Tl[EBqdiCqdX3hckBJfWhvOT2xN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyZYwhemWybHnjZZRqd25iYX\0[ZIhOyCmYYnzJIJ6KHG3YX70bZRifGm4ZTDSWE1RS1JuIFXDOVAhRSByLkSg{txONg>?	M3:0TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUC0NFE{Lz5zOUGwOFAyOzxxYU6=
Huh-9-13	MVzBcpRqfmm{YXygZZN{[Xl?		M3XXXVMh\GG7cx?=	M3XRW2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZZKDl3MD3y[ZNqe3SjboSgTGNXKGenbn;0fZBmKDGkIHnu[oVkfGWmIHnuJIh2dWGwIFj1bE06NTF\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjCzJIRigXNiYomgdZVidnSrdHH0bZZmKFKWLWDDVkwhTUN3MDC9JFAvPDlizszNMi=>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTFyNECxN{c,OTlzMESwNVM9N2F-
Huh-9-13	MmXrRY51cX[rcnHsJIF{e2G7		MVezJIRigXN?	NYPHVlBCSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhWjR5OT3y[ZNqe3SjboSgTGNXKGenbn;0fZBmKDGkIHnu[oVkfGWmIHnuJIh2dWGwIFj1bE06NTF\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjCzJIRigXNiYomgdZVidnSrdHH0bZZmKFKWLWDDVkwhTUN3MDC9JFEvQThizszNMi=>	NGnUUWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGwOFAyOyd-MUmxNFQxOTN:L3G+
Huh-9-13	NWXFOZpxSW62aY\pdoFtKGG\|c3H5		MoPXN{Bl[Xm\|	NITpSZFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBkgWOub4Pwc5JqdmVvcnXzbZN1[W62IFjDWkBo\W6xdInw[UAy[iCrbn\lZ5Rm\CCrbjDoeY1idiCKdXitPU0yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KsJGVEPTBiPTCzMlg2KM7:TT6=	NVzqcWNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNFQxOTNpPkG5NVA1ODF\|PD;hQi=>
A2780/ADR	M4HSUmZ2dmO2aX;uJIF{e2G7			NFHM[nBKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNW2nZHnheIVlKG23bITp[JJ2\yC{ZYPpd5RidmOnIHnuJIFlemmjbYnjbY4uemW\|aYP0ZY51KGi3bXHuJGEzPzhyL1HEVkBk\WyuczDifUBk[WylZXnuJGFOKGG\|c3H5MEBKSzVyIE2gNU41KM7:TT6=	NFPDTms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1NFg{PCd-MUmyOVA5OzR:L3G+
C2C12	MkPaSpVv[3Srb36gZZN{[Xl?	MXmwMlEhfWdxbXy=	MWWzJIRigXN?	Mmm2TY5pcWKrdHnvckBw\iCqZYjvd4UuPi2yaH;zdIhifGViZHXofYRzd2enbnHz[U1qdmS3Y3XkJINmdGxiZHnm[oVz\W62aXH0bY9vKGmwIH3veZNmKEN{Q{GyJINmdGy\|IHHzd4V{e2WmIHHzJI16d3OrbjDo[YF3gSClaHHpckBndHWxcnXzZ4Vv[2ViYYSgNE4yKHWpL33sJIZweiB\|IHThfZMv	M2XwWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEixPFU2Lz5{MEC4NVg2PTxxYU6=
C2C12	NWXuW\|hLTnWwY4Tpc44h[XO\|YYm=			MX\Jcohq[mm2aX;uJI9nKHOncoXtJJdqfGimcnH3ZYwucW6mdXPl[EBk\WyuIHTp[oZmemWwdHnheIlwdiCrbjDtc5V{\SCFMlOxNkBk\WyuczDhd5Nme3OnZDDhd{BugW:\|aX6gbIVifnliY3jhbY4h\XiycnXzd4lwdi5?	NHnGdmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC4NVg2PSd-MkCwPFE5PTV:L3G+
HuH7	M1\pOGFvfGm4aYLhcEBie3OjeR?=		NGnvT3o1QCCqcoO=	NXnK[mY3SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIIP1ZpR6eGViMXKgR49vOSCrbn\lZ5Rm\CCrbjDoeY1idiCKdVi3JINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYLhcEBTVkFibHX2[Ywh[W[2ZYKgOFghcHK\|IHL5JG1VWyCjc4PhfUwhTUN3MDC9JFAvOjFizszNMi=>	Mn35QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzN{[4PVQoRjJyMUe2PFk1RC:jPh?=
HuH7	M2fU[GFvfGm4aYLhcEBie3OjeR?=		NULw[XM2PDhiaILz	M1zrUmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JIN6[2yxc4Dvdolv\SCDLYLld4l{fGGwdDDIR3Yhe3WkdInw[UAy[iCFb36xJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqemGuIGLORUBt\X[nbDDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7LDDFR\|UxKD1iMz6zPUDPxE1w	MoXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzN{[4PVQoRjJyMUe2PFk1RC:jPh?=
T-cells	NIfzNGhKdW23bn;zeZBxemW\|c3n2[UBie3OjeR?=		M3jGXlczKGi{cx?=	NXT4U4ViUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQlHMRk9kKG2xdYPlJHQh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhcpRqNUOGMz;hcpRqNUOGMkitd5RqdXWuYYTl[EBk\WyuIIDyc4xq\mW{YYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODZizszNMi=>	M2fTVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUOxNVA1Lz5{MUGzNVExPDxxYU6=
T-cells	MkfvR5l1d3SxeHnjbZR6KGG\|c3H5		M2LUT\|Q5KGi{cx?=	NHv6ZZJEgXSxdH;4bYNqfHliYXfhbY5{fCCEQVzCM4MhdW:3c3WgWEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDDR\|UxKD1iMUWuPFIh\|ryPLh?=	NFHRSGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzNVExPCd-MkGxN\|EyODR:L3G+
HCT15	MYrGeY5kfGmxbjDhd5NigQ>?	NFXJdYwyOCC3TR?=		MnvLTY5pcWKrdHnvckBw\iCPRGKxJIlvKGi3bXHuJGhEXDF3IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPhcINmcW5vQV2g[YZndHW6IHH0JFExKHWPIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfU4>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2OES2NUc,OjF\|NEi0OlE9N2F-
T-cells	NHfFWY5KdW23bn;zeZBxemW\|c3n2[UBie3OjeR?=		MmTQPVYhcHK\|	NXq3VJdoUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQlHMRk9kN0N3N1LMM\|YhdW:3c3WgWEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHscI9idnSrZ3XuMYlv\HWlZXSgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEm2JIhzeyCkeTDt[YF{fXKrbnegX\|NJZXSqeX3p[Ilv\SC3cIThb4Uh[nlibXn4[YQhdHmvcHjvZ5l1\SC{ZXHjeIlwdiCjc4PhfUwhUUN3MDC9JFAvODF2IN88UU4>	NFvwRWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1OlUzPCd-MkG0OVY2OjR:L3G+
mouse spleen cells	MXPGeY5kfGmxbjDhd5NigQ>?			M1[3[mlvcGmkaYTpc44hd2Zic3XybY5mKHCjbH3peI96dHS{YX7z[oVz[XOnIHnuJI1wfXOnIIPwcIVmdiClZXzsd{4>	NI\UZ3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1OlUzPCd-MkG0OVY2OjR:L3G+
HCT116	NFLGeHBHfW6ldHnvckBie3OjeR?=	NEjNZosyOCC3TR?=	NWHieXJqOzBibXnudy=>	NV;3R21nVW:mdXzheIlwdiCxZjDCR3JRKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IHHjZ5VufWyjdHnvckBw\iCvaYTvfIFvfHKxbnWgZZQhOTBidV2gZYZ1\XJiM{CgcYlveyCkeTDmcI94NWO7dH;t[ZRzgS5?	MmK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2OEi2PFYoRjJzNEi4Olg3RC:jPh?=
Sf21	NUfxdJBPTnWwY4Tpc44h[XO\|YYm=			NUTBfXJ2UW6qaXLpeIlwdiCxZjDoeY1idiCEU1XQJIV5eHKnc4Pl[EBqdiCybHHzcYEhdWWvYoLhcoUhfmW\|aXPs[ZMhd2ZiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHUVE1l\XCnbnTlcpQhYzOKXYTheZJw[2ixbHH0[UB2eHSja3WsJGlEPTBiPTCwMlUh\|ryPLh?=	NGjaUmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm2OVYzOyd-MkG5OlU3OjN:L3G+
Sf21	NYPBOJJSTnWwY4Tpc44h[XO\|YYm=			M2H2cmlvcGmkaYTpc44hd2ZiU4DyZYd2\S2GYYfs[ZkhemG2IFLz[ZAh\XiycnXzd4VlKGmwIIDsZZNu[SCvZX3idoFv\SC4ZYPpZ4xmeyCxZjDT[lIyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gRXRRNWSncHXu[IVvfCCdM1jdeIF2em:laH;sZZRmKHWydHHr[UwhUUN3MDC9JFAvPiEQvF2u	NUKwZpR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OlU3OjNpPkKxPVY2PjJ\|PD;hQi=>
T-cells	NFfWWo9HfW6ldHnvckBie3OjeR?=			NHnXd2hKdmirYnn0bY9vKG:oIFPEOEBmgHC{ZYPzbY9vKGmwIHj1cYFvKEOGNDugWEBk\WyuczygTWM2OCB;IEiuPVch\|ryPLh?=	MmTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMEGxN\|UoRjJ{MUCxNVM2RC:jPh?=
L929	NGXFbGlEgXSxdH;4bYNqfHliYYPzZZk>		NXH4SGtkPzJiaILz	M33FSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw6OjliY3XscJMh[W[2ZYKgO\|IhcHK\|LDDJR\|UxKD1iM{OuPUDPxE1w	NIPhR\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNFQxOid-MkKzNlA1ODJ:L3G+
CCRF-CEM/VCR1000	NELWPGlHfW6ldHnvckBie3OjeR?=		MVKyOFAhe2Wlcx?=	NWj0V2dZUW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdi2vZXTpZZRm\CCmYYXuc5J2[mmlaX6g[YZndHW6IH\yc40hcHWvYX6gR2NTTi2FRV2vWmNTOTByMDDj[YxteyCjZoTldkAzPDBic3Xjd{BjgSCIQVPTJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrczygTWM2OCB;IECuNVAzOzNizszNMi=>	NVvlTIlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OVI1OTJpPkKyOFUzPDF{PD;hQi=>
mouse lymph node cells	NFew[5BKdW23bn;zeZBxemW\|c3n2[UBie3OjeR?=		M3O0eVczKGi{cx?=	NX\hVFk5UW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iYX70bU1ETDNxYX70bU1ETDhiY3:td5RqdXWuYYTl[EBD[WykL3OgcY92e2VibIntdIghdm:mZTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCjZoTldkA4OiCqcoOgZpkhTUyLU1GsJGlEPTBiPTCwMlA4KM7:TT6=	MkT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OUS2NVYoRjJ{NEm0OlE3RC:jPh?=
mouse lymph node cells	MUTDfZRwfG:6aXPpeJkh[XO\|YYm=		MljVOFghcHK\|	NEW0THFEgXSxdH;4bYNqfHliYXfhbY5{fCCEYXziM4MhdW:3c3WgcJlueGhibn;k[UBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDDR\|UxKD1iMUCuNUDPxE1w	MlT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OUS2NVYoRjJ{NEm0OlE3RC:jPh?=
HEK293	NXXRVpR2TnWwY4Tpc44h[XO\|YYm=		NULrV4tLPSCvaX7z	NY\FNHd3UW6qaXLpeIlwdiCxZjDoeY1idiCuaY\ldkBQSVSSMVKzJIV5eHKnc4Pl[EBqdiCKRVuyPVMhTmyyLVnuJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCdM1jdSVE4NWKndHHHJJVxfGGtZTDpcoN2[mG2ZXSg[o9zKDVibXnud{BjgSC\|Y3nueIltdGG2aX;uJINwfW62aX7nMEBMcSB;IEGuNkDPxE1w	Mnz6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NEGwOlgoRjJ{NUSxNFY5RC:jPh?=
HEK293	NE\xTnhHfW6ldHnvckBie3OjeR?=			MUjJcohq[mm2aX;uJI9nKGi3bXHuJIxqfmW{IF;BWHAySjFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDGcJAuUW5iY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGUyPy2kZYThS{B2eHSja3WgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSBzLkOg{txONg>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC2PEc,OjJ3NEGwOlg9N2F-
HEK293	NFfLdIhHfW6ldHnvckBie3OjeR?=		NHKxUZc2KG2rboO=	MmPTTY5pcWKrdHnvckBw\iCqdX3hckBtcX[ncjDPRXRROUJ\|IHX4dJJme3OnZDDpckBJTUt{OUOgSoxxNUmwIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBcO0ifRUG3MYJmfGGJIIXweIFs\SCrbnP1ZoF1\WRiZn;yJFUhdWmwczDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDJR\|UxKD1iMT6zJO69VS5?	M4W2UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	M{W4b2Z2dmO2aX;uJIF{e2G7			M2nBdGlvcGmkaYTpc44hd2ZiaIXtZY4hdGm4ZYKgU2FVWDGEMTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJGZteC2LbjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hTTF5LXLleIFIKHWydHHr[UBjgSC\|Y3nueIltdGG2aX;uJINwfW62aX7nMEBKSzVyIE2gNU41KM7:TT6=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC2PEc,OjJ3NEGwOlg9N2F-
HEK293	NVLicG94TnWwY4Tpc44h[XO\|YYm=		Ml\pOUBucW6\|	NILrfWtKdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFB{QkGg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FN3MhfXC2YXvlJIlv[3WkYYTl[EBnd3JiNTDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurIE2gN\|Yh\|ryPLh?=	NHT4V5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA3QCd-MkK1OFExPjh:L3G+
HEK293	MUXGeY5kfGmxbjDhd5NigQ>?		MXG1JI1qdnN?	M4DhOWlvcGmkaYTpc44hd2ZiaIXtZY4hdGm4ZYKgU2FVWDKEMTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJGZteC2LbjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hYzOKXVWzV{B2eHSja3WgbY5kfWKjdHXkJIZweiB3IH3pcpMh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whUUN3MDC9JFM4KM7:TT6=	NU\oR4NpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFExPjhpPkKyOVQyODZ6PD;hQi=>
T-cells	MljsTY1ufW6xc4XwdJJme3OrdnWgZZN{[Xl?		MmP2NlQhcHK\|	NFPidYxKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCEQVzCM4MhdW:3c3WgWEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPvcmEucW6mdXPl[EBVKGOnbHygdJJwdGmoZYLheIlwdiCrbnP1ZoF1\WRiMkSgbJJ{KHCxc4SgR49vSS2rbnT1Z5Rqd25iZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjF{IN88UU4>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd{N{O2PUc,OjJ5MkezOlk9N2F-
2008	M3HqcGZ2dmO2aX;uJIF{e2G7	M4j1PVExKHWP	MoH1N\|AhdWmwcx?=	NX;QfVZwUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCxJIV5eHKnc4Pl[EBqdiB{MEC4JINmdGy\|IHH0JFExKHWPIIDy[Ylv[3WkYYTl[EBnd3JiM{CgcYlveyCycnnvdkB1dyClYXzj[YlvKEGPIHHk[Il1cW:wIH3lZZN2emWmIHX2[ZJ6KDZyIIPlZ5MhfXBidH:gPVAhdWmwczDifUBk[WylZXnuJGFOKGGlY4XteYxifGmxbjDhd5NigS5?	M{HZZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEG3PFg5Lz5{M{CxO\|g5QDxxYU6=
A2780adr	M3nVbGZ2dmO2aX;uJIF{e2G7	M1jvXlExKHWP	Ml;zN\|AhdWmwcx?=	NFzmW21KdmirYnn0bY9vKG:oIHj1cYFvKFBvZ4Cgc5ZmemW6cILld5Nm\CCrbjDkc5hwenWkaXPpckBz\XOrc4ThcpQhSTJ5OEDh[JIh[2WubIOgZZQhOTBidV2gdJJmcW6ldXLheIVlKG[xcjCzNEBucW6\|IIDybY9zKHSxIHPhcINmcW5iQV2gZYRlcXSrb36gcYVie3W{ZXSg[ZZmenliNkCgd4VkeyC3cDD0c{A6OCCvaX7zJIJ6KGOjbHPlbY4hSU1iYXPjeY12dGG2aX;uJIF{e2G7Lh?=	Ml7qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUe4PFgoRjJ\|MEG3PFg5RC:jPh?=
HL60	MUHGeY5kfGmxbjDhd5NigQ>?	NGriR3Q2KHWP	NX3XTotPPDBibXnudy=>	MVnJcohq[mm2aX;uJI9nKEOjMjutbY5lfWOnZDDtbZRw[2ixbnTybYFtKHCncn3lZYJqdGm2eTD0doFve2m2aX;uJIlvKGi3bXHuJGhNPjBiY3XscJMh[XRiNTD1UUBi\nSncjC0NEBucW6\|IILlcIF1cX[nIITvJINwdnS{b3yu	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{NkC4N{c,OjNyMk[wPFM9N2F-
RPMI1788	MYfJcY12dm:\|dYDwdoV{e2m4ZTDhd5NigQ>?		NVvZToNrPiCmYYnz	NIfwcIVKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckBTWE2LMUe4PEBk\WyuczDjc4N2dHS3cnXkJJdqfGhiaIXtZY4hWEKPQzDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGuzTH11cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjDpcoN2[mG2ZXSg[o9zKDZiZHH5d{BxemmxcjD0c{BcO0ifdHj5cYllcW6nIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDF6IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygTWM2OCB;IECuNFU1KM7:TT6=	NInqU2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O0O\|gxPCd-MkOzOFc5ODR:L3G+
MT4	NEPXXIpCdnSrdnnyZYwh[XO\|YYm=		NUnQPGlFPCCmYYnz	NXj4TG1QSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[2:rbn\lZ5Rm\CC5aYToJGhVVFZzIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYL1d{1qdmS3Y3XkJIN6fG:yYYToc4dmdmmlaYT5JIFnfGW{IESg[IF6eyCkeTDNWHQh[XO\|YYmsJGVEPTBiPTCwMlQ2KM7:TT6=	NGT4V2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0PVg5OCd-MkO4OFk5QDB:L3G+
A2780Adr	M4Poc2Z2dmO2aX;uJIF{e2G7		MmTjN\|AhdWmwcx?=	M3PncGlvcGmkaYTpc44hd2ZicD3ncJlkd3C{b4TlbY4hMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hSTJ5OEDB[JIh[2WubIOgZZN{\XO\|ZXSgZZMh[2GuY3Xpck1CVSCjY3P1cZVt[XSrb36gdJJmcW6ldXLheIVlKG[xcjCzNEBucW6\|IHLl[o9z\SClYXzj[YlvNUGPIHHk[Il1cW:wIH3lZZN2emWmIIXwJJRwKDlyIH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxKD1iMD65PUDPxE1w	M{PDUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEWxNVE1Lz5{M{i1NVEyPDxxYU6=
2008	NFXWOXBHfW6ldHnvckBie3OjeR?=		MkToN\|AhdWmwcx?=	NGD3Zm5KdmirYnn0bY9vKG:oIF3SVFEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hOjByODDj[YxteyCjc4Pld5Nm\CCjczDjZYxk\WmwLVHNJIFk[3WvdXzheIlwdiCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh[mWob4LlJINidGOnaX6tRW0h[WSmaYTpc44hdWWjc4Xy[YQhfXBidH:gPVAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzNjhzIN88UU4>	NVe4dXVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OVEyOTRpPkKzPFUyOTF2PD;hQi=>
Jurkat T	NWfCcY1xTnWwY4Tpc44h[XO\|YYm=		M{SzSlMxKG2rboO=	Mon5TY5pcWKrdHnvckBw\iCQRlHUJIFkfGm4YYTpc44hcW5iUF3BM4lwdm:veXPpck1{fGmvdXzheIVlKGi3bXHuJGp2emujdDDUJINmdGy\|IHnuZ5Vj[XSnZDDmc5IhOzBibXnud{BxemmxcjD0c{BRVUFxaX;uc416[2mwIIP0bY12dGG2aX;uJI1m[XO3cnXkJIFnfGW{IEWgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODBzNDFOwG0v	NFXCR3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2OFk6OSd-MkO5OlQ6QTF:L3G+
MDA435/LCC6MDR	NXmwSZd4TnWwY4Tpc44h[XO\|YYm=			MnrnUY9lfWyjdHnvckBw\iCyLXfwJEh2dmuwb4fuJI9zcWerbjmgeJJidnOoZXP0[YQhcW5iaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiYYPz[ZN{\WRiYYOgdoV3\XK\|YXygc4YheGGlbHn0ZZhmdCC{ZYPpd5RidmOnLDDFR\|UxKD1iMD6wN\|Ih\|ryPLh?=	NYrveWFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|E1PzhpPkK0NVcyPDd6PD;hQi=>
MDA435/LCC6	NHr3TnhEgXSxdH;4bYNqfHliYYPzZZk>		MnfDN{Bl[Xm\|	NWfx[GZnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDNEO1M2xESzZiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOyCmYYnzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDJwODFOwG0v	MnrtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{G0O\|goRjJ2MUexOFc5RC:jPh?=
MDA435/LCC6MDR	M17rXGN6fG:2b4jpZ4l1gSCjc4PhfS=>		NHzZO5A{KGSjeYO=	NWX6cIpXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMzDkZZl{KGK7IF3UV{Bie3OjeTygTWM2OCB;IEiuN{DPxE1w	MmDzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{G0O\|goRjJ2MUexOFc5RC:jPh?=
L929	M33LV2N6fG:2b4jpZ4l1gSCjc4PhfS=>		Mo\SN{Bl[Xm\|	NVf0ZWZwS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVDl{OTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB\|IHThfZMh[nliTWTTJIF{e2G7LDDJR\|UxKD1iM{OuPUDPxE1w	NHnpUGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3NVQ4QCd-MkSxO\|E1Pzh:L3G+
A2780adr	MlPpSpVv[3Srb36gZZN{[Xl?		NFHsZoV2eCC2bzC5NEBucW6\|	MWjJcohq[mm2aX;uJI9nKE2GUkGgbY4hcHWvYX6g[I95d3K3YnnjbY4uemW\|aYP0ZY51KEF{N{iwZYRzKGOnbHzzJIF{e2W\|c3XkJIF{KEOjbHPlbY4hSU1iYXPjeY12dGG2aX;uJJRz\WG2ZXSgN\|AhdWmwczDi[YZwemViQ3HsZ4VqdiCDTTDh[IRqfGmxbjDt[YF{fXKnZDD1dEB1dyB7MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuPVIh\|ryPLh?=	NF3yfmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4OFIyOyd-MkSxPFQzOTN:L3G+
2008	MUPGeY5kfGmxbjDhd5NigQ>?		MV\1dEB1dyB7MDDtbY5{	NUfaV3FmUW6qaXLpeIlwdiCxZjDNVnAyKGmwIHj1cYFvKDJyMEigZ4VtdHNiYYPz[ZN{\WRiYYOgR4Ft[2WrbjDBUUBi[2O3bYXsZZRqd25idILlZZRm\CB\|MDDtbY5{KGKnZn;y[UBE[WylZXnuJGFOKGGmZHn0bY9vKG2nYYP1doVlKHWyIITvJFkxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOi55IN88UU4>	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NEKxN{c,OjRzOESyNVM9N2F-
Saos2	MY\GeY5kfGmxbjDhd5NigQ>?	Mk\FOUB2VQ>?	MX2zJIhzew>?	NXHzfIVnUW6qaXLpeIlwdiCxZjDwMYdxKGmwIHTvfI9zfWKrY3nuMZJme2m\|dHHueEBpfW2jbjDTZY9{OiClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDkc5hwenWkaXPpckB2eHSja3WgZZQhPSC3TTDh[pRmeiB\|IHjyd{BjgSCobH;3JIN6fG:vZYTybYMh[W6jbInzbZMv	Ml;ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MkC0OVIoRjJ2N{KwOFUzRC:jPh?=
Saos2	MVjGeY5kfGmxbjDhd5NigQ>?	NV7pVJdGPSC3TR?=	NYrpV\|NIOiCqcoO=	MV\Jcohq[mm2aX;uJI9nKHBvZ4CgbY4h\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKFOjb4OyJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGmwdILhZ4VtdHWuYYKg[I95d3K3YnnjbY4hemW2ZX70bY9vKGG2IEWgeW0h[W[2ZYKgNkBpenNiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m\|Lh?=	NXvOb2JJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NlA1PTJpPkK0O\|IxPDV{PD;hQi=>
Saos2	M2H6SmZ2dmO2aX;uJIF{e2G7	MVW1JJVO	M3\IUFIhfG9iMzDodpM>	MmfETY5pcWKrdHnvckBw\iCyLXfwJIlvKGSxeH;yeYJq[2mwLYLld4l{fGGwdDDoeY1idiCVYX;zNkBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IHTvfI9zfWKrY3nuJIRqe3S{aXL1eIlwdiCrbjDueYNt\WliYYSgOUB2VSCjZoTldkAzKHSxIEOgbJJ{KGK7IHzhd4VzKHOlYX7ubY5oKGOxbn\vZ4FtKG2rY4Lvd4NweGmlIHHuZYx6e2m\|Lh?=	M365SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{KwOFUzLz5{NEeyNFQ2OjxxYU6=
mouse	NULPOIRuTnWwY4Tpc44h[XO\|YYm=			Mne2TY5pcWKrdHnvckBw\iCqdX3hckBOTFJzIHX4dJJme3OnZDDpckBud3W\|ZTDMOVE4QCClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDv[kBzcG:mYX3pcoUhOTJ\|IHL5JGZCS1OFYXzpZpVzKG[ub4egZ5l1d22ndIL5MEBKSzVyIE2gNE43PyEQvF2u	NXH2ZlY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFAzOjZpPkK0PVAxOjJ4PD;hQi=>
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A2780	NXHHNXo2TnWwY4Tpc44h[XO\|YYm=	NFfYRoE2KHWP	NYHpbnVFOSCqch?=	MWDJcohq[mm2aX;uJI9nKFCpcD3t[YRq[XSnZDDkdpVoKGWoZnz1fEBqdiCjZILpZY16[2mwLYLld4l{fGGwdDDoeY1idiCDMke4NEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCjZILpZY16[2mwIIXweIFs\SCjdDC1JJVOKGGodHXyJFEhcHJiYomg[oxwfyCleYTvcYV1enliYX7hcJl{cXNw	NHn3bFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFMzOyd-MkS5NFA{OjN:L3G+
A2780	Mn\4SpVv[3Srb36gZZN{[Xl?	Mo\uOUB2VQ>?	M{XGWlghcHK\|	NWLsSWhqUW6qaXLpeIlwdiCxZjDQ[5AudWWmaXH0[YQh\HK3ZzDl[oZtfXhiaX6gZYRzcWGveXPpck1z\XOrc4ThcpQhcHWvYX6gRVI4QDBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYRzcWGveXPpckB2eHSja3WgZZQhPSC3TTDh[pRmeiB6IHjyd{BjgSCobH;3JIN6fG:vZYTyfUBidmGueYPpd{4>	MlLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECzNlMoRjJ2OUCwN\|I{RC:jPh?=
T-cells	NXrCbFl1SW62aXnu[oxidW2jdH;yfUBie3OjeR?=	M{\TO\|AvODFidH:gNVAxKG6P	M37KUFIh\GG7cx?=	MUHBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJGxNVzFzODDUR3IhfHKjboPn[Y5q[yCvb4Xz[UBKVC1{LXPvcZBmfGWwdDDDSFQueG:\|aYTpeoUhXCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxNVyBqMUmwJJRwKDJyNTmtbY5lfWOnZDDJUE0zKHC{b3T1Z5Rqd25iYYSgNE4xOSC2bzCxNFAhdk1iZn;yJFIh\GG7czDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm\|aYOu	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEi2Okc,OjR7MEC4OlY9N2F-
Th17	NULleHRSSW62aXnu[oxidW2jdH;yfUBie3OjeR?=	NXnjUnhDOTBidH:gNVAxKG6P	NGPT[HIzKGSjeYO=	MUDBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJGxNVzFzODDUR3IhfHKjboPn[Y5q[yCvb4Xz[UBVcDF5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhUUxvMUegdJJw\HWldHnvckBifCBzMDD0c{AyODBibl2g[o9zKDJiZHH5d{BjgSCobH;3JIN6fG:vZYTybYMh[W6jbInzbZMv	M2LzeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwPFY3Lz5{NEmwNFg3PjxxYU6=
Th1	Mn;FRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>?	NHnRd2QyODBibl2=	NIP4XWgzKGSjeYO=	MnvIRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBNVE9zMUigWGNTKHS{YX7z[4VvcWNibX;1d4UhXGhzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhUU[QLXfhcY1iKHC{b3T1Z5Rqd25iYYSgNVAxKG6PIH\vdkAzKGSjeYOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMi=>	M{L4WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwPFY3Lz5{NEmwNFg3PjxxYU6=
NCI/ADR-RES	NUDET3Y5TnWwY4Tpc44h[XO\|YYm=		M1nIRlIxKG2rboO=	MlG4TY5pcWKrdHnvckBw\iCqdX3hckBRNWeueXPvdJJwfGWrbjDlfJBz\XO\|ZXSgbY4hVkOLL1HEVk1TTVNiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINidGOnaX6tRW0hfHKjboPwc5J1KGGodHXyJFIxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6Ng>?	M1\URVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUmyNVU{Lz5{NEm5NlE2OzxxYU6=
A2780adr	MlHRSpVv[3Srb36gZZN{[Xl?		MnzHPVAhdWmwcx?=	NWDYcmt6UW6qaXLpeIlwdiCxZjDBRmNDOSCrbjDoeY1idiCDMke4NIFleiClZXzsd{BxemVvaX7jeYJifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KGOjbHPlbY4hSU1iYXTkbZRqd25iYX7kJIZ2enSqZYKgbY5kfWKjdHXkJIZweiB4MDDtbY5{KGK7IHPhcINmcW5iQV2gZZN{[XluIFnDOVAhRSByLkmxPUDPxE1w	MonMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6NUW4PVUoRjJ3OEW1PFk2RC:jPh?=
T spleen cells	NUe2bmxUS3m2b4TvfIlkcXS7IHHzd4F6		NHnDXmI4OiCqcoO=	Mo\hR5l1d3SxeHnjbZR6KGGpYXnud5QhUUOUIH3veZNmKFRic4Ds[YVvKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCwMlAyKM7:TT6=	M1u0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[0NlY3Lz5{NkW2OFI3PjxxYU6=
B spleen cells	NHvrSHJEgXSxdH;4bYNqfHliYYPzZZk>		NGHFdW84OiCqcoO=	NWiybYNXS3m2b4TvfIlkcXS7IHHnZYlve3RiSVPSJI1wfXOnIFKgd5Bt\WWwIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB2IN88UU4>	MoDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkSyOlYoRjJ4NU[0NlY3RC:jPh?=
RD	NVvlV3dsSW62aY\pdoFtKGG\|c3H5		M3XxflI1KGi{cx?=	MYTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDFWlcyKGmwZnXjeIVlKGmwIGLEJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOy54NjFOwG0v	M{fyT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[0NlY3Lz5{NkW2OFI3PjxxYU6=
mouse lymph node cells	MUjGeY5kfGmxbjDhd5NigQ>?			MoLyTY5pcWKrdHnvckBw\iCSSUPLMYdidW2jIHnuJI1wfXOnIHz5cZBpKG6xZHWgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFb37BMZN1cW23bHH0[YQhXCClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVAhRSByLkW3JO69VS5?	Ml:2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MkCxOVQoRjJ4N{KwNVU1RC:jPh?=
CD1	MmnNSpVv[3Srb36gZZN{[Xl?	NEfmdZMxNjFidH:gNVAhfU1?	MluxN\|AhdWmwcx?=	MWPJcohq[mm2aX;uJI9nKHSjdYLvcIl1cG:laH;sZZRmKGGlaXSgN{1{fWyoYYTlMYlv\HWlZXSgcoVkem:\|aYOgbY4hS0RzIH3veZNmKHCjbnPy[YF1cWNiYXPpcoFzKGOnbHzzJIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB\|MDDtbY5{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdiYnHz[YQh[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXNw	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl3MEO5Nkc,OjZ7NUCzPVI9N2F-
A2780/ADR	NXTjb492TnWwY4Tpc44h[XO\|YYm=		MmDCN\|AhdWmwcx?=	MYjJcohq[mm2aX;uJI9nKEGEQ1KxJIlvKGi3bXHuJGEzPzhyL1HEVkBk\WyuczDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMhdWWjc4Xy[YQhfXBidH:gN\|YxOCC\|ZXPzJJdqfGhidHnt[UBqdnSnco\hcJMhd2ZiNkCgd4VkeyCkeTDjZYxk\WmwIHHjZ5VufWyjdHnvckBie3OjeTygTWM2OCB;IEGuOFEh\|ryPLh?=	NGCwTlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GwNFA{Oyd-MkexNFAxOzN:L3G+
A2780/ADR	NVnUdmN2S3m2b4TvfIlkcXS7IHHzd4F6		M1PIblczKGi{cx?=	M4HQeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyL1HEVkBk\WyuczDveoVz\XiycnXzd4lv\yCDQlPCNUBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDhwNkOg{txONg>?	M3TKV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUCwNFM{Lz5{N{GwNFA{OzxxYU6=
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Rh30	MmTHdWhVWyCjc4PhfS=>			M{PzeZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpOzBiY3XscJMv	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh41	Ml34dWhVWyCjc4PhfS=>			NWrue\|l3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh2MTDj[Yxtey5?	NILwW4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-MC	NEf5fmpyUFSVIHHzd4F6			M17aWJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzzMi=>	NUOzTGlLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18	M1ywcZFJXFNiYYPzZZk>			M{LSbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|Lh?=	NH7BZ3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-MC	MlvKdWhVWyCjc4PhfS=>			MlOwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtey5?	M1PD[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32	NHjaWIlyUFSVIHHzd4F6			M3fGbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJMv	M{eyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
C57BL/6 mouse lymph node cells	MXfJcY12dm:\|dYDwdoV{e2m4ZTDhd5NigQ>?		NHmx[JEzPCCqcoO=	NGnseXVKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCFNUfCUE83KG2xdYPlJIx6dXCqIH7v[IUh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuOUDPxE1w	NGHrZ\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi3NVg1PCd-Mkm4O\|E5PDR:L3G+
HuH7	M3iwbmFvfGm4aYLhcEBie3OjeR?=		MVKzJIRigXN?	M3vBWGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEXiCpZX7veJlx\SB{YTDpcoZm[3SnZDDpckBpfW2jbjDIeWg4KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDkeYFtNWeubzDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjd5MzFOwG0v	Mn:0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByN{S3PVUoRjNyMEe0O\|k2RC:jPh?=
MT4	NXrtSIV3S3m2b4TvfIlkcXS7IHHzd4F6		NETZTpE1KHSxIEWg[IF6ew>?	MoD3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXQ1KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1KHSxIEWg[IF6eyCkeTDj[YxtNXSrdHXyJIdtdyCjc4PhfUwhS0N3MDC9JFUvPjB6IN88UU4>	NWjwPFhPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwO\|Q4QTVpPkOwNFc1Pzl3PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; PubMed: 12644839 Br J Cancer. Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm). Cyp-D / AIF / β-actin; PubMed: 29328412 Mol Med Rep. Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. P<0.05, P<0.01, **P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor. IL-8 / MCP-1; PubMed: 9602633 Br J Ophthalmol. Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity. Actin / Smac; PubMed: 12427831 J Neurosci. Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release. β-actin / FIPV-N; PubMed: 22546085 Vet Res. Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.	12644839 29328412 9602633 12427831 22546085
Growth inhibition assay	NPC death; PubMed: 20181586 J Neurosci. Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).	20181586
IHC	Th22 / Th17 / Th1 / M1 / Th2; PubMed: 30916387 Clin Exp Immunol. (a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. Tregs / Bregs / pDCs / M2; PubMed: 30916387 Clin Exp Immunol. (b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. Ki 67 / Fas-FasL; PubMed: 30916387 Clin Exp Immunol. (c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. IFN-γ / TNF-α; PubMed: 10931158 Clin Exp Immunol. Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat. WT-PCLS / FXRKO-PCLS; PubMed: 26482353 BMC Genomics. Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).	30916387 10931158 26482353
Immunofluorescence	Human gingival fibroblast cells; PubMed: 27857765 Dent Res J (Isfahan). Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole. Human gingival fibroblast cells; PubMed: 27857765 Dent Res J (Isfahan). Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts. Calcein; PubMed: 23593196 PLoS One. E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm). Rhod-Dex / Calcein; PubMed: 20584761 Toxicol Sci. Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM). cadherin-9 / vimentin; PubMed: 17668045 PLoS One. To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.	27857765 23593196 20584761 17668045

体内研究

Cyclosporin A是一种免疫抑制剂，小鼠，大鼠，和荷兰猪经非肠道给药或者口服给药都有效^[6]，临床上用于器官移植中预防免疫排斥。^[7]

激酶实验：^[3]	- 合并磷酸酶试验: 纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶，100 nM 钙调蛋白，以及5 μM ³²P-标记磷酸化多肽，60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8)，100 mM NaCI，6 mM MgCl₂，0.5 mM 二硫苏糖醇，每毫升0.1毫克牛血清白蛋白，以及0.1 mM CaCl₂或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物， 5 μM ³²P-标记的磷酸肽，和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸，一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca²⁺-不依赖性磷酸酶，而更高浓度时也会部分抑制Ca²⁺-依赖性的活性。30°C下培育15分钟，加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐，用闪烁计数测定数量。
细胞实验：^[3]	- 合并 Cell lines: Jurkat细胞(无性繁殖J77) Concentrations: ~100 nM Incubation Time: 1小时 Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(10⁶)悬浮于微量离心管1ml完全培养基中；1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中，细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次，在50μl低渗缓冲液中裂解，缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯，50 微克胰蛋白酶抑制剂，5微克亮抑酶肽，和5 微克抑肽酶。裂解液用液氮冷冻，再在30°C下融化，循环3次，随后在 4°C下以12,000×g离心10分钟。 (Only for Reference)
动物实验：^[6]	- 合并 Animal Models: 大鼠 Dosages: 45毫克/千克 Administration: 口服 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	100 mg/mL warmed (83.15 mM)
	Water	Insoluble
	Ethanol	''''100 mg/mL warmed
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+5% Tween 80+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Cyclosporin A (NSC 290193) SDF

分子量	1202.61
化学式	C₆₂H₁₁₁N₁₁O₁₂
CAS号	59865-13-3
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	Cyclosporine A, Cyclosporine, Ciclosporin, CsA

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2022-01-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05056779	Not yet recruiting	Drug: Nemolizumab\|Drug: CD14152 placebo	Moderate-to-severe Atopic Dermatitis	Galderma R&D	January 2022	Phase 3
NCT04989686	Not yet recruiting	Other: Microsampling	Immunosuppression	Children''s Hospital of Philadelphia	December 2021	--
NCT04023760	Completed	Drug: Apixaban	Pharmacokinetics\|Kidney Transplant\|Lung Transplant	University of Saskatchewan\|Saskatchewan Health Research Foundation\|Lung Association of Saskatchewan	June 26 2019	Phase 4
NCT03865888	Completed	Drug: Cyclosporins\|Drug: Tacrolimus	Dry Eye\|Sjogren Syndrome	Pavly Moawd\|Cairo University	October 30 2018	Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?
回答：
Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

研究领域

产品类型

定制您的化合物库

Cyclosporin A (NSC 290193)

客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献60篇：

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Cyclosporin A (NSC 290193) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2022-01-17)

技术支持

常见问题及建议解决方法