Cyclosporin A (NSC 290193)

For research use only. Not for use in humans.

目录号:S2286 别名: Cyclosporine A, Cyclosporine, Ciclosporin, CsA 中文名称:环孢菌素A

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1073.15 现货
RMB 786.07 现货
RMB 1957.41 现货
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客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献50篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。
靶点
calcineurin [1]
(Cell-free assay)
7 nM
体外研究

在T细胞中,Cyclosporin A与亲环蛋白(免疫亲和素)结合[1],形成亲环蛋白-Cyclosporin A复合体,其结合并抑制钙调磷酸酶。[2]Cyclosporin A 抑制钙调磷酸酶,IC50 是7 nM[3],随后阻断NF-AT核转运。 [4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体,IC50 是39 nM。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells NH\xdI9EgXSxdH;4bYPDqGG|c3H5 MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiSESySUBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IFHUVEBt\X[nbDygWGM2OD12MjFOwG0> NV;vNG9NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NFIxPThpPkG5OVAzODV6PD;hQi=>
mouse L929 cells Mkj6R5l1d3SxeHnjxsBie3OjeR?= NIf0cGk{KGSjeYO= MkK3R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFkzQSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRUKGG|c3H5MEBKSzVyPUOwJO69VQ>? Mn2xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human Huh-Mono cells NFfq[HNEgXSxdH;4bYPDqGG|c3H5 MUSzJIRigXN? MlnQR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNU2xbn:gZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5Nige,:jDDDR|UxRTJ2IN88US=> NXfHXmtYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
MDCK cells MYHDfZRwfG:6aXRCpIF{e2G7 NEOwR3FEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFPLJINmdGy|LDDUR|UxRTlwOTFOwG0> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
human LCC-6 cells MX3DfZRwfG:6aXRCpIF{e2G7 MWO3NkBp NYjHeHNYS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVEOFLU[gZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUiuN{DPxE1? NYXtZm1kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNlA1ODJpPkKyN|IxPDB{PD;hQi=>
human HeLa cells MUjDfZRwfG:6aXRCpIF{e2G7 MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzMEBVSzVyPUiuN{DPxE1? MojBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
african green monkey Vero cells M365S2Z2dmO2aX;uJIF{e2G7 MkXnNlQhcA>? MX\BcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDX[ZN1KE6rbHWgeolzfXNiaX7m[YN1\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhXmW{bzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckB3cXKjbDDSUmEhe3mwdHjld4l{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:QCEQvF2= NGj6fo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVI5Pid-MUm0OVEzQDZ:L3G+
human Huh-9-13 cells NXHqcpVVS3m2b4TvfIlkyqCjc4PhfS=> NH;DW2Q{KGSjeYO= NUnMeVF2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqLUmtNVMh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3Ojef-8kEBESzVyPUeg{txO M4CyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH6 cells MV\DfZRwfG:6aXRCpIF{e2G7 NHzqNGU{KGSjeYO= NXTQ[ZRzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNjDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCFQ{WwQVch|ryP M{nmN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human Huh5-2 cells MXXDfZRwfG:6aXRCpIF{e2G7 NYPxdnhkOyCmYYnz Moe2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPS1{IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZkhNCCFQ{WwQVYh|ryP M1zMRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH7 cells NYLlPZJSS3m2b4TvfIlkyqCjc4PhfS=> NFvkbJZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IILlcIVie2Vib3[gcIFkfGG2ZTDk[Yh6\HKxZ3XuZZNmNCCLQ{WwQVUvQDFizszN M1;sZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUSzN|kxLz5{MEm0N|M6ODxxYU6=
human 2008 cells MY\GeY5kfGmxbjDhd5NigQ>? MmnyTY5pcWKrdHnvckBw\iCqdX3hckBOWlBzIHnuJIh2dWGwIEKwNFgh[2WubIOsJGlEPTB;ND63PEDPxE1? M1vXTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{C3PFg1Lz5zOEewO|g5PDxxYU6=
human HeLa cells M4S2O2Z2dmO2aX;uJIF{e2G7 Mn;RRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTHBXNTF6IHnu[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB5IHThfZPwxIxiVFO1NF01NjZizszN MnTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
leukemia CEM cells M2r1fGZ2dmO2aX;uJIF{e2G7 MUfJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iTVTSNUBRNUeueXPvdJJwfGWrbjDBRmMhXHKjboPwc5J1\XJidYPpcochdGW3a3XtbYEhS0WPIHPlcIx{NCCLQ{WwQVMvPCEQvF2= M3\UfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN|g4Lz5zMkO2NVM5PzxxYU6=
human Caco2 cells NGfUTWlHfW6ldHnvckBie3OjeR?= M{DVW45pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJGNi[29{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBlcWexeHnuJIVn\my3eDygTWM2OD1{Lkmg{txO NWPWbppwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPFI1PThpPkKzN|gzPDV6PD;hQi=>
human MDA435/LCC6MDR cells NWDqeodXS3m2b4TvfIlkyqCjc4PhfS=> NYTvZnJbPzKq M4XMW2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSTR|NT;MR2M3VUSUIHPlcIx{KGW6cILld5NqdmdiTVTSJIFnfGW{IEeyJIhze+,:jDDJR|UxRTJwODFOwG0> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ|MkC0NFI9N2F-
guinea pig ventricular myocytes MVjGeY5kfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKExvdInw[UBk[WylaYXtJINp[W6wZXygcYVie3W{ZXSgeZNqdmdid3jvcIUu[2WubDDwZZRkcCClbHHtdEBqdiCpdXnu[YEheGmpII\lcpRzcWO3bHHyJI16d2O7dHXzMEBKSzVyPUKg{txO MmexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NkGwNFAoRjJ{N{[xNFAxRC:jPh?=
KB/MDR cell NFLWUZNHfW6ldHnvckBie3OjeR?= MlTxO|IhcA>? NV;S[|hDUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEL13EVkBk\WyuIHzpcoUh[W[2ZYKgO|IhcHJib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0yNjVizszN NESyW5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS4NVk6OSd-MUW0PFE6QTF:L3G+
A2780/ADR cells MmLPSpVv[3Srb36gZZN{[Xl? NGT5d2tKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvKGW6cILld5Nm\CCrbjDBNlc5OC:DRGKgZ4VtdHNiYomgZ4Ft[2WrbjDBUUBie3Ojef-8kEBKSzVyPUGuOFEzPTRizszN NEm1e|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i5NFA6PCd-MUe4PVAxQTR:L3G+
human K562/R7 cells MVTGeY5kfGmxbjDhd5NigQ>? MWWxJO69VQ>? M4XSNVczKGh? MnTPVI91\W62aXH0bY9vKG:oIHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGGpYXnud5Qh\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKEt3NkKvVlch[2WubIOgZZN{\XO|ZXSgZZMh\G:6b4L1ZolkcW5iSVO1NEBifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD63JO69VQ>? NGDPWJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[zOFA1OSd-MkW2N|QxPDF:L3G+
LLC-PK1 epithelial cells MojpSpVv[3Srb36gZZN{[Xl? MkXwTY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYjDlfJBz\XO|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG|c3H589yNKEmFNUC9NE44KM7:TR?= M3Xsc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
mouse L5178 cells NX\PTod6TnWwY4Tpc44h[XO|YYm= MmruNlAhdWmwcx?= MYHJcohq[mm2aX;uJI9nKGi3bXHuJGFDS0JzLX3l[IlifGWmIILoc4RidWmwZTCxNlMh\W[obIX4JIlvKG2xdYPlJGw2OTd6IHPlcIx{KGW6cILld5NqdmdiaIXtZY4hVUSUMTDh[pRmeiB{MDDtbY5{KGK7IF\BR3Mh[W6jbInzbZMtKEmFNUC9NE43PSEQvF2= M4LtZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxO|Q2Lz5{MUO0NVc1PTxxYU6=
KB cell M2DTbmZ2dmO2aX;uJIF{e2G7 NXLPb|ZOUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEIHPlcIwhdGmwZTDh[pRmeiB5MjDoJI9nKGS{dXeg[Zhxd3O3cnWsJGlEPTB;MD62JO69VQ>? NV3qTYlORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFE6QTFpPkG1OFgyQTlzPD;hQi=>
mouse mammary carcinoma cell line EMT6/AR 1.0 MXjGeY5kfGmxbjDhd5NigQ>? NVrPW4JZWmW4ZYLzZYwh\W[oZXP0JI9vKHSqZTDhZ4N2dXWuYYTpc44hd2ZiW{PIYU0h\GG3bn;yeYJq[2mwIHnuJI1wfXOnIH3hcY1ienliY3HyZ4lvd22jIHPlcIwhdGmwZTDFUXQ3N0GUIEGuNEwhUUN3ME2wMlQ1KM7:TR?= NFHtZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEC5PFY4OSd-MUCwPVg3PzF:L3G+
CK1 cells MWjGeY5kfGmxbjDhd5NigQ>? M2DYNGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhRXi1|MzDpcoZm[3SnZDDpckBEUzFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXNuIGTDOVA:OC5|IN88US=> NXXuTHpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyNVIyODBpPkG4NlEzOTByPD;hQi=>
HEK293 cells NHfwVmRHfW6ldHnvckBie3OjeR?= MU\Jcohq[mm2aX;uJI9nKE:DVGCxRlEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KHC{b4TlbY4udWWmaXH0[YQheGm2YY\hd5RifGmwIIXweIFs\e,:jDDLbV0xNjJizszN NHT6[4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4O|k5Pid-MkK1PFc6QDZ:L3G+
human MDA435/LCC6MDR cells M4DoRmZ2dmO2aX;uJIF{e2G7 M4nqPFUh\GG7cx?= NU\kN3AzVW:mdXzheIlwdiCxZjDQMYdxKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gbJVu[W5iTVTBOFM2N0yFQ{\NSHIh[2WubIOgZZN{\XO|ZXSgZZMhemW4ZYLzbY5oKHCjY3zpeIF5\WxicnXzbZN1[W6lZTDt[YF{fXKnZDDhd{Bk\WyuIIP1dpZqfmGuIHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XluIFXDOVA:OC5yM{Kg{txONg>? NITWOZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4OVE6PSd-MkW5PFUyQTV:L3G+
human PBMC M3zFfHBzd2yrZnXyZZRqd25iYYPzZZk> NETs[YxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDCUWMtKEmFNUC9NVIhdk1? M{X2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3Nke5N|A6Lz5zNU[3PVMxQTxxYU6=
mouse T cells MWjGeY5kfGmxbjDhd5NigQ>? NXPXSoVNPCCmYYnz NGjUc2hKdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCvb4Xz[UBVKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geJdwKHejeTDtbZhm\CCueX3wbI9kgXSnIILlZYN1cW:wIHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[XluIHnjOVA:OTFibl2= MknYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OES5PFcoRjJ{OUi0PVg4RC:jPh?=
human splenocytes MlzVSpVv[3Srb36gZZN{[Xl? NYrDN5JRPCCmYYnz MnjtTY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hcHWvYX6gd5Bt\W6xY4n0[ZMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifdHj5cYllcW6nIHnuZ49zeG:{YYTpc44h[W[2ZYKgOEBl[Xm|IHL5JIFtdG:pZX7pZ{BucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJIF{e2G7LDDJR|UxRTFyIH7N MoDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mke4N|EoRjF7OEK3PFMyRC:jPh?=
human T-cell MWnGeY5kfGmxbjDhd5NigQ>? NVTJe285UW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJHQu[2WubDDwdo9lfWO2aX;uJI9nKGy7bYDoc4tqdmViSVytNkwhUUN3ME24MlEhdk1? NUHHdVE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{WzO|M{OSd-N{WzO|M{OTxxYU6=
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MDA-435/LCC6 and MDA-435/LCC6-MDRI MX\GeY5kfGmxbjDhd5NigQ>? MoXKR49v[2WwdILheIlwdiC2aHH0JJJm\HWlZYOg[Iln\mW{ZX7j[UBqdiC{ZY\ldpNidCCxZjDbN2heXkKOIHHjZ5VufWyjdHnvckBj\XS5ZXXuJG1FSS12M{WvUGNEPiCjbnSgUWRCNTR|NT;MR2M3NU2GUlmgZ4VtdHNiYomgOVAmNCCHQ{WwJF0hOC54IN88UU4> MmHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA435/LCC6 MDR1 M{fxW2Z2dmO2aX;uJIF{e2G7 Mn\KdoV3\XK|YXygc4YhXmmwYnzhd5RqdmViYXPjeY12dGG2aX;uJEhXcW6kbHHzeIlv\TpiMD6wNFUhfU1rIHnuJG1FSTR|NT;MR2M3KE2GUkGgZ4VtdHNuIFXDOVAhRSByLk[g{txONg>? MkX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6 (Pgp-negative) MX;GeY5kfGmxbjDhd5NigQ>? MnzmSYZn\WO2IH;uJJJmfmW{c3HsJI9nKESRWDDhZ4N2dXWuYYTpc44hcW5iTVTBMVQ{PS:OQ1O2JEhR\3BvbnXnZZRqfmVrIHPlcIx{NixiRVO1NEA:KDBwNzFOwG0v NYrNRW13RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO|g1OTR|PD;hQi=>
MDA-435/LCC6 Ml7QS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? MX\Hdo94fGhiaX7obYJqfG:{eTDhZ5Rqfmm2eTDvckBOTEFvNEO1M2xESzZiKGDndE1xd3OrdHn2[UkhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{4tKEmFNUCgQUAyKM7:TT6= MlvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6-MDRI NHLvPGdIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NXfLW5B4T3Kxd4ToJIlvcGmkaYTvdpkh[WO2aY\peJkhd25iTVTBMVQ{PS:OQ1O2MW1FWkliKGDndE1v\WejdHn2[UkhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{4tKEmFNUCgQUAyNjFizszNMi=> M1;6SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
BTI-TN5B1-4 NWSyOJI{TnWwY4Tpc44h[XO|YYm= NEXnRnpCXFBiaInkdo9tgXOrczDpckBu\W2kcnHu[UBnemGldHnvckBnem:vIFjp[4ghTmm4ZTCoRnRKNVSQNVKxMVQqKGOnbHzzMEBMdSB;IECuNVch|ryPLh?= NGjaNXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OVY5PCd-MUG3PFU3QDR:L3G+
Jurkat NIKzbY1HfW6ldHnvckBie3OjeR?= NF;RNoNKdmirYnn0bY9vKG:oIIDyc4R2[3Srb36gc4YhUUxvMjDz[YNz\XSrb36gbY4hUnW{a3H0JINmdGy|LDDJR|UxKD1iMD6wNFch|ryPLh?= NYrMUnhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG4PFE6QDRpPkGxPFgyQTh2PD;hQi=>
Jurkat MV7GeY5kfGmxbjDhd5NigQ>? MlXLTY5pcWKrdHnvckBw\iCycn;keYN1cW:wIH;mJIJmfGFvZ3HsZYN1d3OrZHHz[UBKVC1{IGLHRUBqdiCMdYLrZZQh[2WubIOsJGlEPTBiPTCwMlAxQCEQvF2u Mm\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
LLC-PK1 MWjGeY5kfGmxbjDhd5NigQ>? NGDidWhqdmO{ZXHz[UBqdiCYaX7icIF{fGmwZTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUAyNjNizszNMi=> Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7NkGxNVMoRjFzOU[xNVE{RC:jPh?=
LLC-PK1 M4T0OmZ2dmO2aX;uJIF{e2G7 NHTIW4tqdmO{ZXHz[UBqdiCFYXzj[YlvNUGPIHnueJJi[2WubIXsZZIh[WOldX31cIF1cW:wIHnuJG1FWjFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNaTC9JFQvPjZizszNMi=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTl4MUGxN{c,OTF7NkGxNVM9N2F-
MDCK MkfESpVv[3Srb36gZZN{[Xl? M3nITYlvcGmkaYTpc44hd2ZiRHnnc5hqdiC2cnHud4VxcXSqZXzpZYwhfHKjboPwc5J1KCikYYPhcEB1dyCjcHnjZYwqKGmwIF3EVlEu\XiycnXzd4lv\yCPRFPLJINmdGy|LDDLbUA:KDJwMUig{txONg>? MmG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzM{S5OFUoRjF{MUO0PVQ2RC:jPh?=
MDCK M1r1PGZ2dmO2aX;uJIF{e2G7 MWDpcohq[mm2aX;uJI9nKF[rbnLsZZN1cW6nIITyZY5{\XCrdHjlcIlidCC2cnHud5BwenRiKHLhd4FtKHSxIHHwbYNidCliaX6gUXJROi2neIDy[ZN{cW6pIF3ER2sh[2WubIOsJGtqKD1iOD6xNUDPxE1w MlSyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzM{S5OFYoRjF{MUO0PVQ3RC:jPh?=
LLC-PK1 MnPJSpVv[3Srb36gZZN{[Xl? NXfRXIc6cW6lcnXhd4UhcW5iQ3HsZ4Vqdi2DTTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBu\HJzYj3lfJBz\XO|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOy53IN88UU4> NXrUbXFVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN|UzPjdpPkGyNlM2OjZ5PD;hQi=>
LLC-PK1 M{\SWGZ2dmO2aX;uJIF{e2G7 M2\idIlv[3KnYYPlJIlvKEOjbHPlbY4uSU1iaX70doFk\WyudXzhdkBi[2O3bYXsZZRqd25iaX6gcYRzOWFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNaTC9JFMvPjVizszNMi=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjJ|NUK2O{c,OTJ{M{WyOlc9N2F-
HL-60 MmHmSpVv[3Srb36gZZN{[Xl? NFLBdndKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUHWvYX6g[o9zdXmucHXweIll\SC{ZXPldJRweiBqRmDSLUBw\iCqdX3hckBt\XWtZX3pZUBJVC14MDDj[YxteyxiSVOyNEA:KDNwNjFOwG0v Mn:2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ|NkGzPFgoRjF{M{[xN|g5RC:jPh?=
High Five cells NXfCbJJRTnWwY4Tpc44h[XO|YYm= MUfpcohq[mm2aX;uJI9nKFSjdYLvZ4hmdm:mZX;4fYNpd2yjdHWgeZB1[WunKFPzRUA{OHWPLDCzNEAmKG:oIHPvcpRzd2xrIHnuJI1mdWK{YX7lJJZme2mlbHXzJJBz\XCjcnXkJIZzd21iSHnnbEBHcX[nIHPlcIx{KGmwZnXjeIVlKHerdHigeIhmKEGEQ1KxNUBj[WO3bH;2bZJ2eyxiS3mgQUA6NjVizszNMi=> NXHZU|BURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0NFQzOzlpPkGyOFA1OjN7PD;hQi=>
MDCKII M3XleWZ2dmO2aX;uJIF{e2G7 NEPncIpqdmirYnn0bY9vKG:oIFPldol3[XO2YYTpckB2eHSja3WgbY4hV0GWUEKt[ZhxemW|c3nu[{BOTEONSVmgZ4VtdHNuIFvpJF0hOC5{M{ig{txONg>? NIHHPVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkWzPFgyOyd-MUK1N|g5OTN:L3G+
Caco-2 MWPGeY5kfGmxbjDhd5NigQ>? MYT0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IH;mJIRq\2:6aX6gLIJie2GuIITvJIFxcWOjbDmgbY4hS2Glbz2yJINmdGy|LDDLbUA:KDBwM{Wg{txONg>? MlzlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4M{[xOVMoRjF{NkO2NVU{RC:jPh?=
SK-E2 NF3u[5RHfW6ldHnvckBie3OjeR?= NVWzZ3o{cW6lcnXhd4UhcW5iYn;kbZB6KGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKCiEb3TpdJk7KDBwMjD1UUkhcW5iU1utSVIh[2WubIOgLIV5eHKnc4PpcochSlOHUDmsJGlEPTBiPTC3MlUh|ryPLh?= NHHveHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkezPVc2QSd-MUK3N|k4PTl:L3G+
SK-E2 NEHPRmlHfW6ldHnvckBie3OjeR?= MnzhbY5kemWjc3WgbY4h\GmqeXTyc4ZtfW:{ZYPj[YlvKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKCimaXj5[JJw\my3b4Lld4NmcW58IEGgeW0qKGmwIGPLMWUzKGOnbHzzJEhmgHC{ZYPzbY5oKEKVRWCpMEBKSzVyIE2gO{45KM7:TT6= MoDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ5M{m3OVkoRjF{N{O5O|U6RC:jPh?=
HEK293 MkS5SpVv[3Srb36gZZN{[Xl? MorWbY5pcWKrdHnvckBw\iCSaHHscI9q\GmwIIXweIFs\SBqUHjhcIxwcWSrbkqgNUB2VSliaX6gU2FVWC2FLXX4dJJme3OrbnegTGVMOjl|IHPlcIx{NCCNaTC9JFAvOiEQvF2u MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV|MEmwO{c,OTR3M{C5NFc9N2F-
T-cells M2C1dGZ2dmO2aX;uJIF{e2G7 NEfjd2xKdmirYnn0bY9vKG:oIHHscI9o\W6rYzDj[Yxtey2|dHnteYxifGWmIIDyc4xq\mW{YYTpc44hcW5ibX;1d4UhXCClZXzsd{BjgSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKG2ndHjv[EwhUUN3MDC9JFAvODN{IN88UU4> M3:x[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUmzNVgzLz5zNEW5N|E5OjxxYU6=
T-cells MX\GeY5kfGmxbjDhd5NigQ>? MknpTY5pcWKrdHnvckBw\iCjbHzv[4VvcWNiY3XscJMue3SrbYXsZZRm\CCycn;sbYZmemG2aX;uJIlvKGi3bXHuJHQh[2WubIOgZpkhdWm6ZXSgcJlueGixY4n0[UBz\WGldHnvckBu\XSqb3SsJGlEPTBiPTCwMlA4PCEQvF2u M4XKXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUmzNVgzLz5zNEW5N|E5OjxxYU6=
PMBC NFrRO2RHfW6ldHnvckBie3OjeR?= MW[3NkBpenN? M4XhfmVn\mWldDDvckBxem:uaX\ldoF1cW:wIH;mJIh2dWGwIIDldolxcGW{YXygZoxwd2RibX;uc452[2ynYYKgZ4VtdHNiKGDNRmMqKGG2IEeyJIhzKHW|aX7nJG1VXCCjc4PhfUwhUUN3MDC9JFgvQTdizszNMi=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJyM{G2N{c,OTV{MEOxOlM9N2F-
mouse spleen cells NE[4ZmNEgXSxdH;4bYNqfHliYYPzZZk> Mn7OR5l1d3SxeHnjbZR6KGGpYXnud5QhSkGOQj;jJI1wfXOnIIPwcIVmdiClZXzsd{whS0N3MDC9JFEvOzVizszNMi=> MmC3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyOEG3OlEoRjF5MEixO|YyRC:jPh?=
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HEK293 NX3xflQ4TnWwY4Tpc44h[XO|YYm= MmTiTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTUGgUFY2Wi:WTU[gUFM{QUNxVF23JGY4OjhibYX0ZY51KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIEWwKUBqdmirYnn0bY9vKG:oIHPyc5N{KGyrbnvpcoch[nliaX3teY5w[myxdDygRYN1cX[rdImgQUAxNjVizszNMi=> M2XVbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OES4OVY{Lz5zN{i0PFU3OzxxYU6=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; 

PubMed: 12644839     


Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm).

Cyp-D / AIF / β-actin; 

PubMed: 29328412     


Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. *P<0.05, **P<0.01, ***P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor.

IL-8 / MCP-1; 

PubMed: 9602633     


Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity.

Actin / Smac; 

PubMed: 12427831     


Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release.

β-actin / FIPV-N; 

PubMed: 22546085     


Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.

12644839 29328412 9602633 12427831 22546085
Growth inhibition assay
NPC death; 

PubMed: 20181586     


Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).

20181586
IHC
Th22 / Th17 / Th1 / M1 / Th2; 

PubMed: 30916387     


(a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Tregs / Bregs / pDCs / M2; 

PubMed: 30916387     


(b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Ki 67 / Fas-FasL; 

PubMed: 30916387     


(c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

IFN-γ / TNF-α; 

PubMed: 10931158     


Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat.

WT-PCLS / FXRKO-PCLS; 

PubMed: 26482353     


Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).

30916387 10931158 26482353
Immunofluorescence
Human gingival fibroblast cells; 

PubMed: 27857765     


Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole.

Human gingival fibroblast cells; 

PubMed: 27857765     


Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts.

Calcein; 

PubMed: 23593196     


E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm).

Rhod-Dex / Calcein; 

PubMed: 20584761     


Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM).

cadherin-9 / vimentin; 

PubMed: 17668045     


To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.

27857765 23593196 20584761 17668045
体内研究 Cyclosporin A是一种免疫抑制剂,小鼠,大鼠,和荷兰猪经非肠道给药或者口服给药都有效[6],临床上用于器官移植中预防免疫排斥。[7]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[3]
- 合并

磷酸酶试验:

纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶,100 nM 钙调蛋白,以及5 μM 32P-标记磷酸化多肽,60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8),100 mM NaCI,6 mM MgCl2,0.5 mM 二硫苏糖醇, 每毫升0.1毫克牛血清白蛋白,以及0.1 mM CaCl2或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物, 5 μM 32P-标记的磷酸肽,和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸,一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca2+-不依赖性磷酸酶,而更高浓度时也会部分抑制Ca2+-依赖性的活性。30°C下培育15分钟,加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐,用闪烁计数测定数量。
细胞实验:[3]
- 合并
  • Cell lines: Jurkat细胞(无性繁殖J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1小时
  • Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(106)悬浮于微量离心管1ml完全培养基中;1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中,细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次,在50μl低渗缓冲液中裂解,缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯,50 微克胰蛋白酶抑制剂,5微克亮抑酶肽,和5 微克抑肽酶。裂解液用液氮冷冻,再在30°C下融化,循环3次,随后在 4°C下以12,000×g离心10分钟。
    (Only for Reference)
动物实验:[6]
- 合并
  • Animal Models: 大鼠
  • Dosages: 45毫克/千克
  • Administration: 口服
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL warmed (83.15 mM)
Water Insoluble
Ethanol ''''100 mg/mL warmed
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 1202.61
化学式

C62H111N11O12

CAS号 59865-13-3
储存条件 粉状
溶于溶剂
别名 Cyclosporine A, Cyclosporine, Ciclosporin, CsA

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3
NCT01762657 Withdrawn Drug: Oral Cyclosporine and Oral Lansoprazole|Drug: Placebos Type 1 Diabetes Perle Bioscience Inc. September 2014 Phase 3
NCT02130414 Completed Drug: Sandimmun® IV|Drug: CyCol® capsules Healthy Sigmoid Pharma July 2014 Phase 1
NCT01693393 Completed Drug: Cyclosporine A Sjögren´s Syndrome Charite University Berlin Germany March 2010 Phase 2

技术支持

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操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • 回答:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID