Cyclosporin A

For research use only. Not for use in humans.

目录号：S2286 别名： Cyclosporine A 中文名称：环孢菌素A

CAS No. 59865-13-3

Cyclosporin A (Cyclosporine A) 是一种免疫抑制剂，与亲环素结合，随后抑制calcineurin，无细胞试验中IC50为7 nM，广泛用于预防器官移植反应中的排斥反应。

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10mM (1mL in DMSO)	RMB 1073.15	现货
50mg	RMB 786.07	现货
200mg	RMB 1957.41	现货
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客户使用Selleck生产的Cyclosporin A 发表文献40篇：

Cancer Cell, 2018, 33(3):480-494

PubMed: 29533786 ( click the link to review the publication )

Anal Chem, 2020, 10.1021/acs.analchem.0c02756

PubMed: 32933243 ( click the link to review the publication )

Free Radic Biol Med, 2020, 150:109-119

PubMed: 32105828 ( click the link to review the publication )

Toxicol In Vitro, 2020, 8;66:104857

PubMed: 32278034 ( click the link to review the publication )

J Fungi (Basel), 2020, 6(3):E103

PubMed: 32650564 ( click the link to review the publication )

Circulation, 2019, 10.1161/CIRCULATIONAHA.118.037227

PubMed: 31163979 ( click the link to review the publication )

J Clin Invest, 2019, 129(6):2431-2445

PubMed: 31081803 ( click the link to review the publication )

Autophagy, 2019, 15(3):493-509

PubMed: 30304977 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-19-1982

PubMed: 31582380 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):487

PubMed: 31822296 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2019, 14:192-203

PubMed: 30623853 ( click the link to review the publication )

Front Immunol, 2019, 10:2952

PubMed: 31921197 ( click the link to review the publication )

J Antimicrob Chemother, 2019, 74(7):1921-1927

PubMed: 30934052 ( click the link to review the publication )

Oxid Med Cell Longev, 2019, 2019:4248529

PubMed: 30881590 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(10):6897-6906

PubMed: 31379115 ( click the link to review the publication )

Front Microbiol, 2019,

PubMed: 32038504 ( click the link to review the publication )

J Med Virol, 2019, 91(8):1440-1447

PubMed: 30900754 ( click the link to review the publication )

Med Sci Monit, 2019,

PubMed: 30913205 ( click the link to review the publication )

Biochim Biophys Acta Gene Regul Mech, 2019, 1862(8):846-857

PubMed: 31054961 ( click the link to review the publication )

Nat Commun, 2018, 9(1):873

PubMed: 29491374 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(23):6066-6077

PubMed: 30061362 ( click the link to review the publication )

Oncogenesis, 2018, 7(11):91

PubMed: 30467308 ( click the link to review the publication )

Sci Rep, 2018, 8(1):10910

PubMed: 30026610 ( click the link to review the publication )

Chem Res Toxicol, 2018, 31(2):127-136

PubMed: 29156121 ( click the link to review the publication )
Sci Adv, 2018, 4(8):eaas9784

PubMed: 30101190 ( click the link to review the publication )

Nat Cell Biol, 2017, 19(10):1274-1285

PubMed: 28892080 ( click the link to review the publication )

Elife, 2017, 6e26733

PubMed: 28737489 ( click the link to review the publication )

J Neurochem, 2017, 143(3):282-293

PubMed: 28902407 ( click the link to review the publication )

Int J Cardiol, 2017, 227:201-208

PubMed: 27839819 ( click the link to review the publication )

Am J Physiol Renal Physiol, 2017, 313(2):F404-F413

PubMed: 28381458 ( click the link to review the publication )

PLoS One, 2017, 12(10):e0187123

PubMed: 29073235 ( click the link to review the publication )

J Mol Neurosci, 2017, 61(2):267-278

PubMed: 28054226 ( click the link to review the publication )

Pharm Res, 2016, 33(2):395-403

PubMed: 26415646 ( click the link to review the publication )

Oncotarget, 2016, 7(6):6711-26

PubMed: 26744319 ( click the link to review the publication )

Oncogene, 2015, 10.1038/onc.2015.32

PubMed: 25728673 ( click the link to review the publication )

Antiviral Res, 2015, 120:140-6

PubMed: 26086883 ( click the link to review the publication )

Drug Metab Dispos, 2014, 42(3):318-22

PubMed: 24335466 ( click the link to review the publication )

Arch Environ Con Tox, 2014, 68(1):31-7

PubMed: 25194241 ( click the link to review the publication )

Biochim Biophys Acta, 2013, 1833(3):652-62

PubMed: 23228564 ( click the link to review the publication )

Drug Metab Dispos, 2013, 42(3):318-22

PubMed: 24335466 ( click the link to review the publication )

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述

靶点

calcineurin ^[1]
(Cell-free assay)

7 nM

体外研究

在T细胞中，Cyclosporin A与亲环蛋白(免疫亲和素)结合^[1]，形成亲环蛋白-Cyclosporin A复合体，其结合并抑制钙调磷酸酶。^[2]Cyclosporin A 抑制钙调磷酸酶，IC50 是7 nM^[3]，随后阻断NF-AT核转运。 ^[4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体，IC50 是39 nM。^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
rat H42E cells	M{DCWWN6fG:2b4jpZ:Kh[XO\|YYm=			MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiSESySUBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IFHUVEBt\X[nbDygWGM2OD12MjFOwG0>	NH\IcHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWwNlA2QCd-MUm1NFIxPTh:L3G+
mouse L929 cells	MYPDfZRwfG:6aXRCpIF{e2G7		NV33SJhPOyCmYYnz	MoS5R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFkzQSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRUKGG\|c3H5MEBKSzVyPUOwJO69VQ>?	M4XobFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human Huh-Mono cells	NEDhXGREgXSxdH;4bYPDqGG\|c3H5		M2rwWFMh\GG7cx?=	MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYguVW:wbzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F697zOIFPDOVA:OjRizszN	NWPJR49YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
MDCK cells	M1LKd2N6fG:2b4jpZ:Kh[XO\|YYm=			NEnKUHJEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFPLJINmdGy\|LDDUR\|UxRTlwOTFOwG0>	MmTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells	M{C2TGN6fG:2b4jpZ:Kh[XO\|YYm=		NH\CZXk4OiCq	MmG4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUGNENTZiY3XscJMh[W[2ZYKgO\|IhcHK\|LDDJR\|UxRThwMzFOwG0>	Ml;yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|MkC0NFIoRjJ{M{KwOFAzRC:jPh?=
human HeLa cells	M4PMfGN6fG:2b4jpZ:Kh[XO\|YYm=			M33UTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMtKFSFNUC9PE4{KM7:TR?=	M4fKRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells	M1rTSWZ2dmO2aX;uJIF{e2G7		NIj0cmEzPCCq	MVPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDX[ZN1KE6rbHWgeolzfXNiaX7m[YN1\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhXmW{bzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckB3cXKjbDDSUmEhe3mwdHjld4l{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:QCEQvF2=	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUK4Okc,OTl2NUGyPFY9N2F-
human Huh-9-13 cells	MmfvR5l1d3SxeHnjxsBie3OjeR?=		NH:2WWc{KGSjeYO=	NHPiXYFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvOT2xN{Bk\WyuczDh[pRmeiB\|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;NzFOwG0>	NHTKOoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human HuH6 cells	M4HBNWN6fG:2b4jpZ:Kh[XO\|YYm=		MoD0N{Bl[Xm\|	NGnk[HJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh4IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEOFNUC9O{DPxE1?	NHfQO\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human Huh5-2 cells	NXvTdFRFS3m2b4TvfIlkyqCjc4PhfS=>		NGCxPXY{KGSjeYO=	M1fZfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2cDVvMjDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6KCxiQ1O1NF03KM7:TR?=	NIHYSVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human HuH7 cells	NWPzWoM{S3m2b4TvfIlkyqCjc4PhfS=>			MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W\|c3XkJIF{KHKnbHXhd4Uhd2ZibHHjeIF1\SCmZXj5[JJw\2WwYYPlMEBKSzVyPUWuPFEh\|ryP	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl2M{O5NEc,OjB7NEOzPVA9N2F-
human 2008 cells	NGe0UXRHfW6ldHnvckBie3OjeR?=			MXTJcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD12Lke4JO69VQ>?	NFHZbVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEewO\|g5PCd-MUi3NFc5QDR:L3G+
human HeLa cells	M{HBVGZ2dmO2aX;uJIF{e2G7			NVPHPGc1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUG4JIlv\mWldHXkJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXQxvJygWGM2OD12Lk[g{txO	MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
leukemia CEM cells	NEDPd4FHfW6ldHnvckBie3OjeR?=			NHrKc2VKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUHWvYX6gUWRTOSCSLVfsfYNweHKxdHXpckBCSkNiVILhcpNxd3K2ZYKgeZNqdmdibHX1b4VucWFiQ1XNJINmdGy\|LDDJR\|UxRTNwNDFOwG0>	M2WxPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN\|g4Lz5zMkO2NVM5PzxxYU6=
human Caco2 cells	M1jDcWZ2dmO2aX;uJIF{e2G7			NWLIT\|J4dmirYnn0bY9vKG:oIGCt[5AhcW5iaIXtZY4hS2Glb{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGSrZ3;4bY4h\W[obIX4MEBKSzVyPUKuPUDPxE1?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN6MkS1PEc,OjN\|OEK0OVg9N2F-
human MDA435/LCC6MDR cells	NHvxNYlEgXSxdH;4bYPDqGG\|c3H5		NWmxWJMzPzKq	MlnMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR\|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=>	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ\|MkC0NFI9N2F-
guinea pig ventricular myocytes	Mln3SpVv[3Srb36gZZN{[Xl?			M4fVPGlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG\|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1?	NEjNWJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2NVAxOCd-MkK3OlExODB:L3G+
KB/MDR cell	MVLGeY5kfGmxbjDhd5NigQ>?		MV63NkBp	MVjJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgT2IwVUSUIHPlcIwhdGmwZTDh[pRmeiB5MjDodkBw\iCmcoXnJIV5eG:\|dYLlMEBKSzVyPUGuOUDPxE1?	M3SxR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
A2780/ADR cells	Mn;BSpVv[3Srb36gZZN{[Xl?			Mn7ETY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckBmgHC{ZYPz[YQhcW5iQUK3PFAwSUSUIHPlcIx{KGK7IHPhcINmcW5iQV2gZZN{[XoxvJygTWM2OD1zLkSxNlU1KM7:TR?=	NWX3SWhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells	NV7K[5RlTnWwY4Tpc44h[XO\|YYm=	M3;sSVEh\|ryP	NVK1b3NJPzJiaB?=	MonjVI91\W62aXH0bY9vKG:oIHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGGpYXnud5Qh\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKEt3NkKvVlch[2WubIOgZZN{\XO\|ZXSgZZMh\G:6b4L1ZolkcW5iSVO1NEBifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD63JO69VQ>?	NF;XWVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[zOFA1OSd-MkW2N\|QxPDF:L3G+
LLC-PK1 epithelial cells	MWnGeY5kfGmxbjDhd5NigQ>?			MomwTY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYjDlfJBz\XO\|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG\|c3H589yNKEmFNUC9NE44KM7:TR?=	NWXmfJBGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
mouse L5178 cells	NGTJblVHfW6ldHnvckBie3OjeR?=		NHW0eI0zOCCvaX7z	NE\jUoxKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1KxMY1m\GmjdHXkJJJpd2SjbXnu[UAyOjNiZX\mcJV5KGmwIH3veZNmKEx3MUe4JINmdGy\|IHX4dJJme3OrbnegbJVu[W5iTVTSNUBi\nSncjCyNEBucW6\|IHL5JGZCS1NiYX7hcJl{cXNuIFnDOVA:OC54NTFOwG0>	NVTSfm9zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFE4PDVpPkKxN\|QyPzR3PD;hQi=>
KB cell	Ml\tSpVv[3Srb36gZZN{[Xl?			Mnm0TY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDKGOnbHygcIlv\SCjZoTldkA4OiCqIH;mJIRzfWdiZYjwc5N2emVuIFnDOVA:OC54IN88US=>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MUm5NUc,OTV2OEG5PVE9N2F-
mouse mammary carcinoma cell line EMT6/AR 1.0	M4q2fGZ2dmO2aX;uJIF{e2G7			NH2xZmNT\X[ncoPhcEBm\m[nY4Sgc44hfGinIHHjZ5VufWyjdHnvckBw\iCdM1jdMUBl[XWwb4L1ZolkcW5iaX6gcY92e2VibXHtcYFzgSClYYLjbY5wdWFiY3XscEBtcW6nIFXNWFYwSVJiMT6wMEBKSzVyPUCuOFQh\|ryP	NXvnVXBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwPVg3PzFpPkGwNFk5PjdzPD;hQi=>
CK1 cells	MluwSpVv[3Srb36gZZN{[Xl?			Ml\3RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTHBXNTN\|IHnu[oVkfGWmIHnuJGNMOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEeg[IF6eyxiVFO1NF0xNjNizszN	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
HEK293 cells	M3vjXGZ2dmO2aX;uJIF{e2G7			MXfJcohq[mm2aX;uJI9nKE:DVGCxRlEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KHC{b4TlbY4udWWmaXH0[YQheGm2YY\hd5RifGmwIIXweIFs\e,:jDDLbV0xNjJizszN	NV;FfXNWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFc6QDZpPkKyOVg4QTh4PD;hQi=>
human MDA435/LCC6MDR cells	M1XON2Z2dmO2aX;uJIF{e2G7		NFXqUoo2KGSjeYO=	NG\XW2FOd2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N\|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpcocheGGlbHn0ZZhmdCC{ZYPpd5RidmOnIH3lZZN2emWmIHHzJINmdGxic4Xyeol3[WxiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5NigSxiRVO1NF0xNjB\|MjFOwG0v	M1vnNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC	NIXQXoZRem:uaX\ldoF1cW:wIHHzd4F6			MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCETVOsJGlEPTB;MUKgcm0>	NHi0fGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[3PVMxQSd-MUW2O\|k{ODl:L3G+
mouse T cells	M3zCR2Z2dmO2aX;uJIF{e2G7		NG\hRWE1KGSjeYO=	NWrkZmtlUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5ibX;1d4UhXCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJR4dyC5YYmgcYl5\WRibIntdIhw[3m2ZTDy[YFkfGmxbjDh[pRmeiB2IHThfZMh[nliTWTUJIF{e2G7LDDpZ\|UxRTFzIH7N	MnPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OES5PFcoRjJ{OUi0PVg4RC:jPh?=
human splenocytes	MkPhSpVv[3Srb36gZZN{[Xl?		MmjUOEBl[Xm\|	NEDtVlVKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckB{eGynbn;jfZRmeyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFt\|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCjZoTldkA1KGSjeYOgZpkh[Wyub3flcolkKG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44h[XO\|YYmsJGlEPTB;MUCgcm0>	Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mke4N\|EoRjF7OEK3PFMyRC:jPh?=
human T-cell	NFfuSY5HfW6ldHnvckBie3OjeR?=			MV;JckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVD3j[YxtKHC{b3T1Z5Rqd25ib3[gcJlueGixa3nu[UBKVC1{LDDJR\|UxRThwMTDuUS=>	MlzZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzV\|N{OzNUc,PzV\|N{OzNVww[T5?
human Jurkat cells	NVf0cYdQTnWwY4Tpc44h[XO\|YYm=		MUKxJIg>	MnyxRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDKeZJs[XRiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidnSrLVPEN{9ETDJ6IHHueIljd2S7LXnu[JVk\WRiSVytNkBxem:mdXP0bY9vKHS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJIFvfGlvQ1SzM2NFOjhiYX70bYJw\HliY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJIJ6KGmvbYXuc5N2eHC{ZYPzbY9vKGG\|c3H5MEBGSzVyPUWgcm0>	M4LiO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOzO\|k2Lz5zOUmzN\|c6PTxxYU6=
human PBMC	NX3sUFU4TnWwY4Tpc44h[XO\|YYm=		NY\xcIpROSCq	NX\aR3ZySW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBRSk2FIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYX70bU1ETDNxQ1SyPEBidnSrYn;kfU1qdmS3Y3XkJGlNNTJicILv[JVkfGmxbjD0doVifGWmIH\vdkAyKGi{IHLl[o9z\SCjboTpMWNFOy:FREK4JIFvfGmkb3T5JINp[WyuZX7n[UBu\WG\|dYLl[EBi\nSncjC2JIhzeyCkeTDpcY12dm:\|dYDwdoV{e2mxbjDhd5NigSxiRVO1NF0{NjFibl2=	NIrTR5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzN\|c6PSd-MUm5N\|M4QTV:L3G+
Jurkat cells	Mn\VSpVv[3Srb36gZZN{[Xl?			Ml:1TY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImge4F{KG2nYYP1doVlKGK7IHnubIljcXSrb36gc4YhfGinIFnMMVIheHKxZIXjeIlwdiCrbjDKeZJs[XRiY3XscJMtKEmFNUC9NkBvVQ>?	M2jFNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NkSzN\|M4Lz5zNE[0N\|M{PzxxYU6=
HepG2 cells	NI\3UXJHfW6ldHnvckBie3OjeR?=			M2O0SGlvcGmkaYTpc44hd2ZibHn2[ZIhe3SjZ3WgVIxie22xZHn1cUBj\XKpaHXpJIlv\mWldHnvckBqdiCKZYDHNkBk\WyuczygTWM2OD1zLk[1JI5O	MnTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OE[xNlQoRjJ{NUi2NVI1RC:jPh?=
Jurkat	Mm[zSpVv[3Srb36gZZN{[Xl?			NHTBTG5VcGViY3;tdI92dmRid3HzJJRme3SnZDDpckB3cXS{bzDmc5IhcW6qaXLpeIlwdiCxZjDJUE0zKHKnbHXhd4Uhe3SrbYXsZZRm\CC5aYToJHBOSS:rb37vcZlkcW5iYomgTpVzc2G2IHPlcIx{NixiSVO1NEA:KDBwMEGyJO69VS5?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84PDd\|NUSzK\|44PDd\|NUSzQE9iRg>?
mouse spleen cells	NGq1e5RHfW6ldHnvckBie3OjeR?=			M2\ycnRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBqdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44hcW5iQlTGNUBud3W\|ZTDzdIxm\W5iY3XscJMhe3SrbYXsZZRm\CC5aYToJINwdmOjbnH2ZYxqdiCDIDjDc45CMS5uIFnDOVAhRSByLkC0JO69VS5?	NFPEd\|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95NEezOVQ{Lz55NEezOVQ{RC:jPh?=
Sf9	MmC3SpVv[3Srb36gZZN{[Xl?			MoXsbY5pcWKrdHnvckBw\iCYZYLhdIFucWxic4TpcZVt[XSnZDDBWHAhcHmmcn;sfZNqeyCrbjDt[Y1jemGwZYOg[pJwdSCPRGKxMYV5eHKnc4PpcochW2Z7IHPlcIx{NCCNaTC9JFAvODJizszNMi=>	NE\MOmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95NUG0NlY{Lz55NUG0NlY{RC:jPh?=
LLC-PK1	MUjGeY5kfGmxbjDhd5NigQ>?			NUS3XZNRfHKjboPldIl1cGWuaXHsJJRz[W6\|cH;yeEAp[mG\|YXygeI8h[XCrY3HsLUBqdiCPRGKxMYV5eHKnc4PpcochVEyFLWDLNUBk\WyuczygT40hRSB6LkSg{txONg>?	NHf4Z3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95NkixNFU6Lz55NkixNFU6RC:jPh?=
HeLa	NFi2R\|RHfW6ldHnvckBie3OjeR?=			MX\pcohq[mm2aX;uJI9nKEyWQ{SgeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHWg[pJwdSCPUmCxMYV5eHKnc4PpcochUGWOYTDj[YxteyxiS3mgQUA2KM7:TT6=	M3npSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd7NkG3NFYoRjd7NkG3NFY9N2F-
Caco-2	NW[2emFmTnWwY4Tpc44h[XO\|YYm=			MVX0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIHnuJGNi[29vMjDj[YxteyxiS32gQUA{NjhizszNMi=>	NVi1UpJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEi0NlQ2Oid-OEi0NlQ2OjxxYU6=
spleen	M4DafmZ2dmO2aX;uJIF{e2G7			NULmZZUzTW[oZXP0JI9nKGOxbYDveY5lKG:wIFnMMVIheHKxZIXjeIlwdiCocn;tJIFtdG:jboTp[4VvNXO2aX31cIF1\WRibX;1d4Uhe3CuZXXuJINmdGy\|LDDJR\|UxKD1iMD6wNUDPxE1w	M2rRXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh6OUO4N\|koRjh6OUO4N\|k9N2F-
COS	NHrQbZVHfW6ldHnvckBie3OjeR?=			MXHpcohq[mm2aX;uJI9nKFSjdYLvZ4hwdGG2ZTD1dJRic2ViaX6gRXNDXC2neIDy[ZN{cW6pIFPPV{Bk\WyuczygT4khRSB{NDFOwG0v	NVqwS5YzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUS1PFc5PSd-OUS1PFc5PTxxYU6=
LLC-PK1	MkDOSpVv[3Srb36gZZN{[Xl?			M1jqT4lvcGmkaYTpc44hd2ZiRH;4c5J2[mmlaX6geJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTCoSIlod3irbkqgNE45KHWPKTDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiSVO1NEA:KDNwNk[g{txONg>?	M{XVRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl7MUS3PVIoRjl7MUS3PVI9N2F-
CEM/VLB100	NH7pc4xHfW6ldHnvckBie3OjeR?=			M2W1folvcGmkaYTpc44hd2ZiQ3HsZ4Vqdi2DTTDl[oZtfXhiaX6gR2VON1[OQkGwNEBk\WyuczygT4khRSBzLkig{txONg>?	M1HW[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMUm4NlI4Lz5zMEG5PFIzPzxxYU6=
HEK	MVHGeY5kfGmxbjDhd5NigQ>?			NIjiTplqdmirYnn0bY9vKG:oIFLNS{B2eHSja3WgbY4hdWWvYoLhcoUhfmW\|aXPs[ZMh\nKxbTDNdpAzNWW6cILld5NqdmdiSFXLJINmdGy\|LDDLbUA:KDFyIN88UU4>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODR{MU[1PEc,OTB2MkG2OVg9N2F-
HEK	NXzoc4ZKTnWwY4Tpc44h[XO\|YYm=			NEniTZNqdmirYnn0bY9vKG:oIFLNS{B2eHSja3WgbY4hdWWvYoLhcoUhfmW\|aXPs[ZMh\nKxbTDNVnAzNWW6cILld5NqdmdiSFXLJINmdGy\|LDDLbUA:KDJzIN88UU4>	M1zGZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNEKxOlU5Lz5zMESyNVY2QDxxYU6=
HeLa	NUTBbpdRTnWwY4Tpc44h[XO\|YYm=			NXH2UIZGcW6qaXLpeIlwdiCxZjDUZZVzd2Oqb3zheIUhfXC2YXvlJIlvKE6WQ2Ct[ZhxemW\|c3nu[{BJ\UyjIHPlcIx{NCCLQ{WwJF0hOSEQvF2u	M1TycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNU[1PFQ{Lz5zMEW2OVg1OzxxYU6=
LLC-PK1	NXPNPZVFTnWwY4Tpc44h[XO\|YYm=			NWXuNYMycW6qaXLpeIlwdiCxZjDMWGM1KHWydHHr[UBqdiCvZX3idoFv\SC4ZYPpZ4xmeyCocn;tJG1TWDJvZYjwdoV{e2mwZzDMUGMhWEtzIHPlcIx{NCCNaTC9JFQvPyEQvF2u	M2C2eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNUewNFQ6Lz5zMEW3NFA1QTxxYU6=
Sf9	NIfBXJhHfW6ldHnvckBie3OjeR?=			MULpcohq[mm2aX;uJI9nKFSjdYLvZ4hwdGG2ZTD1dJRic2ViaX6gcYVu[nKjbnWgeoV{cWOuZYOg[pJwdSCEc3XwMYV5eHKnc4PpcochW2Z7IHPlcIx{NCCNaTC9JFAvOyEQvF2u	NE\ZXVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[0PFQ4OCd-MUC2OFg1PzB:L3G+
Caco-2	M2PYR2Z2dmO2aX;uJIF{e2G7			M170bIlvcGmkaYTpc44hd2ZiRHnnc5hqdiC2cnHud4VxcXSqZXzpZYwhfHKjboPwc5J1KCikYYPhcEB1dyCjcHnjZYwqKCiGaXfvfIlvQiB3IIXNLUBqdiCFYXPvMVIh[2WubIOsJGlEPTBiPTCxMlMh\|ryPLh?=	NVLZOWlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4NlAyOzdpPkGwPFIxOTN5PD;hQi=>
CEM/VLB100	NGnHWHNHfW6ldHnvckBie3OjeR?=			NWPCdWhocW6qaXLpeIlwdiCxZjDDZYxk\WmwLVHNJIVn\my3eDCoR4Ft[2Wrbj3BUVohOC5{NTD1UUkhcW5iQ1XNM3ZNSjFyMDDj[YxteyxiSVO1NEA:KDNwNEGg{txONg>?	NInVTm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEi5NVEyPCd-MUC4PVEyOTR:L3G+
Sf9	MlrVSpVv[3Srb36gZZN{[Xl?			MXnpcohq[mm2aX;uJI9nKEGWUHHz[UBqdiCvZX3idoFv\SC4ZYPpZ4xmKG[{b32gRmNTWC2neIDy[ZN{cW6pIGPmPUBk\WyuczygT4khRSByLkWg{txONg>?	NIi4No09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUSzO\|M5OCd-MUG0N\|c{QDB:L3G+
NIH-3T3-G185	NULNNnFuTnWwY4Tpc44h[XO\|YYm=			Ml7LbY5pcWKrdHnvckBw\iCGYYXuc5J2[mmlaX6g[YZndHW6IHnuJG5KUC1\|VEOtS\|E5PSClZXzsd{whUUN3MDC9JFEvPCEQvF2u	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	M3\hfWZ2dmO2aX;uJIF{e2G7			NVzSUpZ5cW6qaXLpeIlwdiCxZjDDZYxk\WmwLVHNJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUCgQUAyNjdizszNMi=>	MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	M3PQXmZ2dmO2aX;uJIF{e2G7			M3HMXolvcGmkaYTpc44hd2ZiUnjv[IFucW6nIEGyN{Bm\m[udYigbY4hVkmKLUPUN{1IOTh3IHPlcIx{NCCLQ{WwJF0hOS55IN88UU4>	MmPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	MoLsSpVv[3Srb36gZZN{[Xl?			M2DsZYlvcGmkaYTpc44hd2ZiTFTTMVc2OSCnZn\seZghcW5iTlnIMVNVOy2JMUi1JINmdGy\|LDDJR\|UxKD1iMT64JO69VS5?	MmnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	M3jmPGZ2dmO2aX;uJIF{e2G7			M2r0VIlvcGmkaYTpc44hd2ZiVHX0doFu\XSqeXzyc5NidWmwZTDl[oZtfXhiaX6gUmlJNTOWMz3HNVg2KGOnbHzzMEBKSzVyIE2gNk4yKM7:TT6=	NXfMe2lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NVY2OTRpPkGxO\|E3PTF2PD;hQi=>
NIH-3T3-G185	MXTGeY5kfGmxbjDhd5NigQ>?			NGfIOXlqdmirYnn0bY9vKG:oIF\seY8uOy2DTTDl[oZtfXhiaX6gUmlJNTOWMz3HNVg2KGOnbHzzMEBKSzVyIE2gNk41KM7:TT6=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	NH\Pc4RHfW6ldHnvckBie3OjeR?=			MnfRbY5pcWKrdHnvckBw\iCMQz2xJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUCgQUA1NjNizszNMi=>	M{S5[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{G2OVE1Lz5zMUexOlUyPDxxYU6=
HCT15	M1PEOWZ2dmO2aX;uJIF{e2G7	Mo\FNE41KHSxIE[uN{B2\y:vTB?=		NETjWIJOd2S3bHH0bY9vKG:oIGCt[5AhcW5iaIXtZY4hUEOWMUWgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hTEmQSVKtbY5lfWOnZDDjfZRwfG:6aXPpeJkh[XRiMD60JJRwKDZwMzD1[{9uVC5?	M2jlb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{W0OlAzLz5zMUe1OFYxOjxxYU6=
UO31	MXTGeY5kfGmxbjDhd5NigQ>?	Mmq0NE41KHSxIE[uN{B2\y:vTB?=		NGL3b4ZOd2S3bHH0bY9vKG:oIGCt[5AhcW5iaIXtZY4hXU9\|MTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBFUU6LQj3pcoR2[2WmIHP5eI91d3irY3n0fUBifCByLkSgeI8hPi5\|IIXnM41NNg>?	NID0W\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe1OFYxOid-MUG3OVQ3ODJ:L3G+
CAKI1	M2HvfmZ2dmO2aX;uJIF{e2G7	MVSwMlQhfG9iNj6zJJVoN22O		MYfNc4R2dGG2aX;uJI9nKFBvZ4CgbY4hcHWvYX6gR2FMUTFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gSGlPUUJvaX7keYNm\CCleYTveI95cWOrdImgZZQhOC52IITvJFYvOyC3Zz;tUE4>	NV\rV5RYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3OVQ3ODJpPkGxO\|U1PjB{PD;hQi=>
MDA-435/LCC6 and MDA-435/LCC6-MDRI	NXrUR2dXTnWwY4Tpc44h[XO\|YYm=			MmK3R49v[2WwdILheIlwdiC2aHH0JJJm\HWlZYOgeIhmKGSrZn\ldoVv[2ViaX6gdoV3\XK\|YXygc4YhTE:[IHHjZ5VufWyjdHnvckBj\XS5ZXXuJG1FSS12M{WvUGNEPiCjbnSgUWRCNTR\|NT;MR2M3NU2GUlmgZ4VtdHNiYomgOVAmNixiRVO1NEA:KDBwNTFOwG0v	NUfvPHN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO\|g1OTR\|PD;hQi=>
MDA-435/LCC6	M2TWSWZ2dmO2aX;uJIF{e2G7			MVXF[oZm[3Rib36gdoV3\XK\|YXygc4YhYzOKXT2gWmJNKGGlY4XteYxifGmxbjDpckBOTEFvNEO1M2xESzZiKGDndE1v\WejdHn2[Ukh[2WubIOuMEBGSzVyIE2gNE43KM7:TT6=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
MDA-435/LCC6 and MDA-435/LCC6-MDRI	MV7GeY5kfGmxbjDhd5NigQ>?			MY\Dc45k\W62cnH0bY9vKHSqYYSgdoVlfWOnczDkbYZn\XKnbnPlJIlvKHKndnXyd4FtKG:oIGuzTH1XSkxiYXPjeY12dGG2aX;uJIJmfHenZX6gUWRCNTR\|NT;MR2M3KGGwZDDNSGEuPDN3L1zDR\|YuVUSUSTDj[YxteyCkeTC1NEUtKEWFNUCgQUAxNjZizszNMi=>	M1fL[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA435/LCC6 MDR1	NYHaUGVLTnWwY4Tpc44h[XO\|YYm=			NHe2TmZz\X[ncoPhcEBw\iCYaX7icIF{fGmwZTDhZ4N2dXWuYYTpc44hMF[rbnLsZZN1cW6nOjCwMlAxPSC3TTmgbY4hVUSDNEO1M2xESzZiTVTSNUBk\WyuczygSWM2OCB;IECuOkDPxE1w	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
MDA-435/LCC6 (Pgp-negative)	M3;MSmZ2dmO2aX;uJIF{e2G7			MnPKSYZn\WO2IH;uJJJmfmW{c3HsJI9nKESRWDDhZ4N2dXWuYYTpc44hcW5iTVTBMVQ{PS:OQ1O2JEhR\3BvbnXnZZRqfmVrIHPlcIx{NixiRVO1NEA:KDBwNzFOwG0v	M37CbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA-435/LCC6	M3rRZ2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7			NIO4eFhIem:5dHigbY5pcWKrdH;yfUBi[3Srdnn0fUBwdiCPRFGtOFM2N0yFQ{[gLHBoeC2yb4PpeIl3\SliaIXtZY4h[nKnYYP0JINidmOncjDj[Yxtey5uIFnDOVAhRSBzIN88UU4>	NXy0OFMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO\|g1OTR\|PD;hQi=>
MDA-435/LCC6-MDRI	MVfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>?			NWDxZ5U6T3Kxd4ToJIlvcGmkaYTvdpkh[WO2aY\peJkhd25iTVTBMVQ{PS:OQ1O2MW1FWkliKGDndE1v\WejdHn2[UkhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{4tKEmFNUCgQUAyNjFizszNMi=>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
BTI-TN5B1-4	NXT2S2lPTnWwY4Tpc44h[XO\|YYm=			NHPXUJNCXFBiaInkdo9tgXOrczDpckBu\W2kcnHu[UBnemGldHnvckBnem:vIFjp[4ghTmm4ZTCoRnRKNVSQNVKxMVQqKGOnbHzzMEBMdSB;IECuNVch\|ryPLh?=	NELRZ\|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OVY5PCd-MUG3PFU3QDR:L3G+
Jurkat	MUDGeY5kfGmxbjDhd5NigQ>?			M{XoOmlvcGmkaYTpc44hd2ZicILv[JVkfGmxbjDv[kBKVC1{IIPlZ5JmfGmxbjDpckBLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkCwO{DPxE1w	MkfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
Jurkat	NELmUVdHfW6ldHnvckBie3OjeR?=			MmDUTY5pcWKrdHnvckBw\iCycn;keYN1cW:wIH;mJIJmfGFvZ3HsZYN1d3OrZHHz[UBKVC1{IGLHRUBqdiCMdYLrZZQh[2WubIOsJGlEPTBiPTCwMlAxQCEQvF2u	MnTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
LLC-PK1	M{fLe2Z2dmO2aX;uJIF{e2G7			NH7XeIVqdmO{ZXHz[UBqdiCYaX7icIF{fGmwZTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUAyNjNizszNMi=>	M1;FbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[xNVE{Lz5zMUm2NVEyOzxxYU6=
LLC-PK1	M33BfWZ2dmO2aX;uJIF{e2G7			MXrpcoNz\WG\|ZTDpckBE[WylZXnuMWFOKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKGmwIF3EVlEu\XiycnXzd4lv\yCOTFOtVGsyKGOnbHzzMEBMcSB;IESuOlYh\|ryPLh?=	NHfj[Fg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUm2NVEyOyd-MUG5OlEyOTN:L3G+
MDCK	MlfySpVv[3Srb36gZZN{[Xl?			NH:0S4xqdmirYnn0bY9vKG:oIFTp[495cW5idILhcpNmeGm2aHXsbYFtKHS{YX7zdI9zfCBqYnHzZYwhfG9iYYDpZ4FtMSCrbjDNSHIyNWW6cILld5NqdmdiTVTDT{Bk\WyuczygT4khRSB{LkG4JO69VS5?	NILaR2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGzOFk1PSd-MUKxN\|Q6PDV:L3G+
MDCK	MVHGeY5kfGmxbjDhd5NigQ>?			MnTPbY5pcWKrdHnvckBw\iCYaX7icIF{fGmwZTD0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIHnuJG1TWDJvZYjwdoV{e2mwZzDNSGNMKGOnbHzzMEBMcSB;IEiuNVEh\|ryPLh?=	M4TMW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUO0PVQ3Lz5zMkGzOFk1PjxxYU6=
LLC-PK1	MVjGeY5kfGmxbjDhd5NigQ>?			MUHpcoNz\WG\|ZTDpckBE[WylZXnuMWFOKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKGmwIH3kdlFjNWW6cILld5NqdmdiTFzDMXBMOSClZXzsd{whU2liPTCzMlUh\|ryPLh?=	NUHa[W9nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN\|UzPjdpPkGyNlM2OjZ5PD;hQi=>
LLC-PK1	MnLlSpVv[3Srb36gZZN{[Xl?			MlPLbY5kemWjc3WgbY4hS2GuY3Xpck1CVSCrboTyZYNmdGy3bHHyJIFk[3WvdXzheIlwdiCrbjDt[JIy[S2neIDy[ZN{cW6pIFzMR{1RUzFiY3XscJMtKEurIE2gN{43PSEQvF2u	M1znd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MkO1NlY4Lz5zMkKzOVI3PzxxYU6=
HL-60	Mn7NSpVv[3Srb36gZZN{[Xl?			MkjITY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIH\vdo16dHCncITp[IUhemWlZYD0c5IhME[SUjmgc4YhcHWvYX6gcIV2c2WvaXGgTGwuPjBiY3XscJMtKEmFMkCgQUA{NjZizszNMi=>	MkLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ\|NkGzPFgoRjF{M{[xN\|g5RC:jPh?=
High Five cells	M2HUVWZ2dmO2aX;uJIF{e2G7			NIfr[ldqdmirYnn0bY9vKG:oIGTheZJw[2inbn;k[Y95gWOqb3zheIUhfXC2YXvlLGN{SSB\|MIXNMEA{OCBnIH;mJINwdnS{b3ypJIlvKG2nbXLyZY5mKH[nc3njcIV{KHC{ZYDhdoVlKG[{b32gTIlocCCIaY\lJINmdGy\|IHnu[oVkfGWmIIfpeIghfGinIFHCR2IyOSCkYXP1cI93cXK3czygT4khRSB7LkWg{txONg>?	Ml7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MESyN\|koRjF{NEC0NlM6RC:jPh?=
MDCKII	MkPGSpVv[3Srb36gZZN{[Xl?			NV\xdGw4cW6qaXLpeIlwdiCxZjDD[ZJqfmG\|dHH0bY4hfXC2YXvlJIlvKE:DVGCyMYV5eHKnc4PpcochVUSFS1nJJINmdGy\|LDDLbUA:KDBwMkO4JO69VS5?	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjV\|OEixN{c,OTJ3M{i4NVM9N2F-
Caco-2	M2HpWmZ2dmO2aX;uJIF{e2G7			NHnGS411emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JI9nKGSrZ3;4bY4hMGKjc3HsJJRwKGGyaXPhcEkhcW5iQ3Hjc{0zKGOnbHzzMEBMcSB;IECuN\|Uh\|ryPLh?=	MnKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4M{[xOVMoRjF{NkO2NVU{RC:jPh?=
SK-E2	MnTsSpVv[3Srb36gZZN{[Xl?			MUjpcoNz\WG\|ZTDpckBjd2SrcImgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gLGJw\GmyeUqgNE4zKHWPKTDpckBUUy2HMjDj[YxteyBqZYjwdoV{e2mwZzDCV2VRMSxiSVO1NEA:KDdwNTFOwG0v	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjd\|OUe1PUc,OTJ5M{m3OVk9N2F-
SK-E2	NYf0TmN3TnWwY4Tpc44h[XO\|YYm=			MoPHbY5kemWjc3WgbY4h\GmqeXTyc4ZtfW:{ZYPj[YlvKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKCimaXj5[JJw\my3b4Lld4NmcW58IEGgeW0qKGmwIGPLMWUzKGOnbHzzJEhmgHC{ZYPzbY5oKEKVRWCpMEBKSzVyIE2gO{45KM7:TT6=	MkLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ5M{m3OVkoRjF{N{O5O\|U6RC:jPh?=
HEK293	M3zHTGZ2dmO2aX;uJIF{e2G7			NFywbnBqdmirYnn0bY9vKG:oIGDoZYxtd2mmaX6geZB1[WunIDjQbIFtdG:rZHnuPkAyKHWPKTDpckBQSVSSLVOt[ZhxemW\|c3nu[{BJTUt{OUOgZ4VtdHNuIFvpJF0hOC5{IN88UU4>	MlrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3M{C5NFcoRjF2NUOwPVA4RC:jPh?=
T-cells	MmPqSpVv[3Srb36gZZN{[Xl?			M1mwVGlvcGmkaYTpc44hd2ZiYXzsc4dmdmmlIHPlcIx{NXO2aX31cIF1\WRicILvcIln\XKjdHnvckBqdiCvb4Xz[UBVKGOnbHzzJIJ6KG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44hdWW2aH;kMEBKSzVyIE2gNE4xOzJizszNMi=>	NVT5bZExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1PVMyQDJpPkG0OVk{OTh{PD;hQi=>
T-cells	M17TZWZ2dmO2aX;uJIF{e2G7			Mn;zTY5pcWKrdHnvckBw\iCjbHzv[4VvcWNiY3XscJMue3SrbYXsZZRm\CCycn;sbYZmemG2aX;uJIlvKGi3bXHuJHQh[2WubIOgZpkhdWm6ZXSgcJlueGixY4n0[UBz\WGldHnvckBu\XSqb3SsJGlEPTBiPTCwMlA4PCEQvF2u	MlXNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3OUOxPFIoRjF2NUmzNVgzRC:jPh?=
PMBC	Mke1SpVv[3Srb36gZZN{[Xl?		MWK3NkBpenN?	MXrF[oZm[3Rib36gdJJwdGmoZYLheIlwdiCxZjDoeY1idiCyZYLpdIhmemGuIHLsc49lKG2xbn;ueYNt\WG{IHPlcIx{KCiSTVLDLUBifCB5MjDodkB2e2mwZzDNWHQh[XO\|YYmsJGlEPTBiPTC4Mlk4KM7:TT6=	NGTGfXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUKwN\|E3Oyd-MUWyNFMyPjN:L3G+
mouse spleen cells	MmnOR5l1d3SxeHnjbZR6KGG\|c3H5			NYXBRpFSS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJJNxdGWnbjDj[YxteyxiQ1O1NEA:KDFwM{Wg{txONg>?	NYTRbVJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewPFE4PjFpPkG3NFgyPzZzPD;hQi=>
MDCK2	NWrxWWw1TnWwY4Tpc44h[XO\|YYm=			MUPJcohq[mm2aX;uJI9nKGi3bXHuJG1FWjFvZHXw[Y5l\W62IHHjZ5VufWyjdHnvckBw\iClYXzj[YlvNUGPIHX4dJJme3OnZDDpckBOTEONMjDj[YxteyxiSVO1NEA:KDlwM{Sg{txONg>?	NHv2e3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[2OFMzPyd-MUe2OlQ{Ojd:L3G+
HEK293	M4HROWZ2dmO2aX;uJIF{e2G7			MX7Jcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPMTDMOlVTN1SPNjDMN\|M6Sy:WTUegSlczQCCvdYThcpQh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdiNUClJIlvcGmkaYTpc44hd2ZiY4Lvd5MhdGmwa3nu[{BjgSCrbX31co9jdG:2LDDBZ5Rqfmm2eTC9JFAvPSEQvF2u	NF21PXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i0PFU3Oyd-MUe4OFg2PjN:L3G+
HEK293	M1\0d2Z2dmO2aX;uJIF{e2G7			NHnKZoVKdmirYnn0bY9vKG:oIHPyc5N{NWyrbnvpcochd2ZiaIXtZY4hWGeyIGTNOkBNOzN7Qz;UUVchTjd{OFOgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW\|aX7nJFUxLSCrbnjpZol1cW:wIH;mJINzd3O\|IHzpcotqdmdiYomgbY1ufW6xYnzveEwhSWO2aY\peJkhRSByLk[g{txONg>?	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293	NVfkXm1QTnWwY4Tpc44h[XO\|YYm=			MVfJcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPM2SxPVlTN1SPNjDMN\|M6Sy:WTUegSlczQENibYX0ZY51KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO\|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIEWwKUBqdmirYnn0bY9vKG:oIHPyc5N{KGyrbnvpcoch[nliaX3teY5w[myxdDygRYN1cX[rdImgQUAyNjFizszNMi=>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293	NIHyS5JHfW6ldHnvckBie3OjeR?=			MVLJcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPNjDGN\|Q{Wi:WTU[gUFM{QUNxVF23JGY4OjiFIH31eIFvfCCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHTyeYchdGW4ZXygZ4F2e2mwZzC1NEUhcW6qaXLpeIlwdiCxZjDjdo9{eyCuaX7rbY5oKGK7IHntcZVvd2Kub4SsJGFkfGm4aYT5JF0hOTFizszNMi=>	Ml7OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6NEi1OlMoRjF5OES4OVY{RC:jPh?=
HEK293	MVzGeY5kfGmxbjDhd5NigQ>?			Ml7TTY5pcWKrdHnvckBw\iClcn;zd{1tcW6taX7nJI9nKGi3bXHuJHBoeCCWTUWgTVMxPlJxVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDF3IN88UU4>	Ml;aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6NEi1OlMoRjF5OES4OVY{RC:jPh?=
MDCK2	M4TIWWZ2dmO2aX;uJIF{e2G7	MkXHNVAhfU1?	M1rH[lQxKG2rboO=	MWjJcohq[mm2aX;uJI9nKGi3bXHuJJdqdGRidInw[UBR\3BiZYjwdoV{e2WmIHnuJG1FS0t{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPhcINmcW5vQV2gZYNkfW23bHH0bY9vKGG2IEGwJJVOKGGodHXyJFQxKG2rboOu	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl4NEG3NEc,OTd7NkSxO\|A9N2F-
A2780	MmXTSpVv[3Srb36gZZN{[Xl?		MmntN\|AhdWmwcx?=	NGC0e3NKdmirYnn0bY9vKG:oIHj1cYFvKFCpcDDpckBCOjd6MDDj[YxteyCjZoTldkA{OCCvaX7zJIJ6KEixZXPod5QhOzN\|NEKgZZN{[XluIFnDOVAhRSBzLkSxNlU1KM7:TT6=	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6M{CzOEc,OThyOEOwN\|Q9N2F-
A2780	MUPGeY5kfGmxbjDhd5NigQ>?		M1r3XVMxKG2rboO=	NFzY[nRKdmirYnn0bY9vKG:oIHj1cYFvKFCpcDDpckBCOjd6MDDj[YxteyCjZoTldkA{OCCvaX7zJIJ6KGOjbHPlbY4hSU1iYYPzZZktKEmFNUCgQUAyNjRzMkW0JO69VS5?	NIe5[mI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4N\|A{PCd-MUiwPFMxOzR:L3G+
Caco-2	M2ruV2Z2dmO2aX;uJIF{e2G7			M1u4bmlvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4udWWmaXH0[YQhYzOKXY\pcoJt[XO2aX7lJJRz[W6\|cH;yeEBqdiCqdX3hckBE[WOxLUKgZ4VtdHNuIFXDOVAhRSB2NT61JO69VS5?	NF7EZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWyOFU6Oid-MUi1NlQ2QTJ:L3G+
A2780	MlvqSpVv[3Srb36gZZN{[Xl?			NFXNdppKdmirYnn0bY9vKG:oIGCt[5AhcW5iaIXtZY4h[WS{aXHtfYNqdi2{ZYPpd5RidnRiQUK3PFAh[2WubIOgZpkhUG:nY3jzeEA{OzN2MjDhd5NigSxiSVO1NEA:KDFwNEGyOVQh\|ryPLh?=	NYDtfGZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2O\|g1QTVpPkG4Olc5PDl3PD;hQi=>
Huh-9-13	M1LLcmFvfGm4aYLhcEBie3OjeR?=		NHjzRo0{KGSjeYO=	MUHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDCTWxPKDJyNkGtdoV{cXO2YX70JGhEXiCpZX7veJlx\SBzYjDpcoZm[3SnZDDpckBpfW2jbjDIeYguQS1zMzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgN{Bl[Xm\|IHL5JJF2[W62aYTheIl3\SCUVD3QR3ItKEWFNUCgQUAxNjByNDFOwG0v	MoTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	Mof0RY51cX[rcnHsJIF{e2G7		MX:zJIRigXN?	NEfmUGZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBkd22yb4Xu[E1z\XOrc4ThcpQhUEOYIHflco91gXCnIEHiJIlv\mWldHXkJIlvKGi3bXHuJGh2cC17LUGzJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA{KGSjeYOgZpkheXWjboTpeIF1cX[nIGLUMXBEWixiRVO1NEA:KDBwM{Wg{txONg>?	M3rXXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUC0NFE{Lz5zOUGwOFAyOzxxYU6=
Huh-9-13	MV\BcpRqfmm{YXygZZN{[Xl?		MoDzN{Bl[Xm\|	NEGxcWNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEAzLy2FLX3leIh6dC1{Jz3mcJVwem9vY4n0bYRqdmVvcnXzbZN1[W62IFjDWkBo\W6xdInw[UAy[iCrbn\lZ5Rm\CCrbjDoeY1idiCKdXitPU0yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KsJGVEPTBiPTCwMlQh\|ryPLh?=	Mn21QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	M4rUeWFvfGm4aYLhcEBie3OjeR?=		Mn7hN{Bl[Xm\|	MV7BcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDWXEA6PTBvcnXzbZN1[W62IFjDWkBo\W6xdInw[UAy[iCrbn\lZ5Rm\CCrbjDoeY1idiCKdXitPU0yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KsJGVEPTBiPTCwMlQ6KM7:TT6=	Ml7jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	M{nRcWFvfGm4aYLhcEBie3OjeR?=		NYTFcVF{OyCmYYnz	NF;DXXdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBTPDd7LYLld4l{fGGwdDDIR3Yh\2Wwb4T5dIUhOWJiaX7m[YN1\WRiaX6gbJVu[W5iSIXoMVkuOTNiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEOg[IF6eyCkeTDxeYFvfGm2YYTpeoUhWlRvUFPSMEBGSzVyIE2gNU46QCEQvF2u	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTFyNECxN{c,OTlzMESwNVM9N2F-
Huh-9-13	MXfBcpRqfmm{YXygZZN{[Xl?		Mn2wN{Bl[Xm\|	NXG4SXZFSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5Qh[3mlbH;zdI9zcW6nLYLld4l{fGGwdDDIR3Yh\2Wwb4T5dIUhOWJiaX7m[YN1\WRiaX6gbJVu[W5iSIXoMVkuOTNiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEOg[IF6eyCkeTDxeYFvfGm2YYTpeoUhWlRvUFPSMEBGSzVyIE2gN{45PSEQvF2u	M1XDV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUC0NFE{Lz5zOUGwOFAyOzxxYU6=
A2780/ADR	Mk[5SpVv[3Srb36gZZN{[Xl?			NXqzd3J[UW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdi2vZXTpZZRm\CCvdXz0bYRzfWdicnXzbZN1[W6lZTDpckBi\HKrYX35Z4lvNXKnc3nzeIFvfCCqdX3hckBCOjd6MD;BSHIh[2WubIOgZpkh[2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFEvPCEQvF2u	NIS3RlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1NFg{PCd-MUmyOVA5OzR:L3G+
C2C12	MnXtSpVv[3Srb36gZZN{[Xl?	Ml7ENE4yKHWpL33s	NIHOeJg{KGSjeYO=	NHvOU21KdmirYnn0bY9vKG:oIHjlfI9{\S14LYDoc5NxcGG2ZTDk[Yh6\HKxZ3XuZZNmNWmwZIXj[YQh[2WubDDkbYZn\XKnboTpZZRqd25iaX6gcY92e2ViQ{LDNVIh[2WubIOgZZN{\XO\|ZXSgZZMhdXmxc3nuJIhm[X[7IHPoZYlvKG[udX;y[ZNk\W6lZTDheEAxNjFidXevcYwh\m:{IEOg[IF6ey5?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB6MUi1OUc,OjByOEG4OVU9N2F-
C2C12	M2L6WGZ2dmO2aX;uJIF{e2G7			NWLPSFhUUW6qaXLpeIlwdiCxZjDz[ZJ2dSC5aYTo[JJif2GuLXnu[JVk\WRiY3XscEBlcW[oZYLlcpRq[XSrb36gbY4hdW:3c3WgR\|JEOTJiY3XscJMh[XO\|ZYPz[YQh[XNibYnvd4lvKGinYY\5JINp[WmwIHX4dJJme3Orb36u	NV2yVWxKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwPFE5PTVpPkKwNFgyQDV3PD;hQi=>
HuH7	MV;BcpRqfmm{YXygZZN{[Xl?		MX20PEBpenN?	M4nGTmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEXiC\|dXL0fZBmKDGkIFPvclEhcW6oZXP0[YQhcW5iaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygVm5CKGyndnXsJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO\|YYmsJGVEPTBiPTCwMlIyKM7:TT6=	M1LPflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUe2PFk1Lz5{MEG3Olg6PDxxYU6=
HuH7	M{X5NmFvfGm4aYLhcEBie3OjeR?=		MXy0PEBpenN?	NFPCe\|RCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBkgWOub4Pwc5JqdmViQT3y[ZNqe3SjboSgTGNXKHO3YoT5dIUhOWJiQ3;uNUBqdm[nY4Tl[EBqdiCqdX3hckBJfUh5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckB3cXKjbDDSUmEhdGW4ZXygZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygSWM2OCB;IEOuN\|kh\|ryPLh?=	MnTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzN{[4PVQoRjJyMUe2PFk1RC:jPh?=
T-cells	M12ye2ludXWwb4P1dJBz\XO\|aY\lJIF{e2G7		NVTE[nFwPzJiaILz	NWfEW5c6UW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQlHMRk9kKG2xdYPlJHQh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhcpRqNUOGMz;hcpRqNUOGMkitd5RqdXWuYYTl[EBk\WyuIIDyc4xq\mW{YYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODZizszNMi=>	MmqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzM{GxNFQoRjJzMUOxNVA1RC:jPh?=
T-cells	MXzDfZRwfG:6aXPpeJkh[XO\|YYm=		NXrEdI1VPDhiaILz	MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBDSUyEL3OgcY92e2ViVDDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCFQ{WwJF0hOTVwOEKg{txONg>?	NF25Vpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzNVExPCd-MkGxN\|EyODR:L3G+
HCT15	M2ry[2Z2dmO2aX;uJIF{e2G7	MVKxNEB2VQ>?		M4TV[2lvcGmkaYTpc44hd2ZiTVTSNUBqdiCqdX3hckBJS1RzNTDj[YxteyCjc4Pld5Nm\CCjczDjZYxk\WmwLVHNJIVn\my3eDDheEAyOCC3TTDifUBndHWxcnXzZ4Vv[2ViYYPzZZkv	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2OES2NUc,OjF\|NEi0OlE9N2F-
T-cells	NHnIZ5lKdW23bn;zeZBxemW\|c3n2[UBie3OjeR?=		MlTpPVYhcHK\|	NWT2NnR[UW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQlHMRk9kN0N3N1LMM\|YhdW:3c3WgWEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHscI9idnSrZ3XuMYlv\HWlZXSgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEm2JIhzeyCkeTDt[YF{fXKrbnegX\|NJZXSqeX3p[Ilv\SC3cIThb4Uh[nlibXn4[YQhdHmvcHjvZ5l1\SC{ZXHjeIlwdiCjc4PhfUwhUUN3MDC9JFAvODF2IN88UU4>	M4jHN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEW2OVI1Lz5{MUS1OlUzPDxxYU6=
mouse spleen cells	MUPGeY5kfGmxbjDhd5NigQ>?			Mn;5TY5pcWKrdHnvckBw\iC\|ZYLpcoUheGGubXn0c5ltfHKjboPm[ZJie2ViaX6gcY92e2Vic4Ds[YVvKGOnbHzzMi=>	MlK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NU[1NlQoRjJzNEW2OVI1RC:jPh?=
HCT116	MnnzSpVv[3Srb36gZZN{[Xl?	M2P1cFExKHWP	MVqzNEBucW6\|	MWrNc4R2dGG2aX;uJI9nKEKFUmCgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnueJJi[2WubIXsZZIh[WOldX31cIF1cW:wIH;mJI1qfG:6YX70do9v\SCjdDCxNEB2VSCjZoTldkA{OCCvaX7zJIJ6KG[ub4etZ5l1d22ndIL5Mi=>	NFn5Z3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS4PFY5Pid-MkG0PFg3QDZ:L3G+
Sf21	M1zWTGZ2dmO2aX;uJIF{e2G7			M2PwZmlvcGmkaYTpc44hd2ZiaIXtZY4hSlOHUDDlfJBz\XO\|ZXSgbY4heGyjc33hJI1mdWK{YX7lJJZme2mlbHXzJI9nKFOoMkGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCDVGCt[IVx\W6mZX70JHs{UF22YYXyc4Npd2yjdHWgeZB1[WunLDDJR\|UxKD1iMD61JO69VS5?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl4NU[yN{c,OjF7NkW2NlM9N2F-
Sf21	NIHMZY9HfW6ldHnvckBie3OjeR?=			MlPYTY5pcWKrdHnvckBw\iCVcILh[5VmNUSjd3zlfUBz[XRiQoPldEBmgHC{ZYPz[YQhcW5icHzhd41iKG2nbXLyZY5mKH[nc3njcIV{KG:oIGPmNlEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDBWHAu\GWyZX7k[Y51KFt\|SG30ZZVzd2Oqb3zheIUhfXC2YXvlMEBKSzVyIE2gNE43KM7:TT6=	MoP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
T-cells	NVPKdGVoTnWwY4Tpc44h[XO\|YYm=			MXzJcohq[mm2aX;uJI9nKEOGNDDlfJBz\XO\|aX;uJIlvKGi3bXHuJGNFPCtiVDDj[YxteyxiSVO1NEA:KDhwOUeg{txONg>?	NXHJe2xMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFEyOzVpPkKyNVAyOTN3PD;hQi=>
L929	NGTycodEgXSxdH;4bYNqfHliYYPzZZk>		NYDId5lkPzJiaILz	NX\sdmU{S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVDl{OTDj[YxteyCjZoTldkA4OiCqcoOsJGlEPTBiPTCzN{46KM7:TT6=	M1[zVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
CCRF-CEM/VCR1000	NIHJfFZHfW6ldHnvckBie3OjeR?=		Ml;xNlQxKHOnY4O=	NHT4[\|VKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNW2nZHnheIVlKGSjdX7vdpVjcWOrbjDl[oZtfXhiZoLvcUBpfW2jbjDDR3JHNUOHTT;WR3IyODByIHPlcIx{KGGodHXyJFI1OCC\|ZXPzJIJ6KE[DQ2Og[oxwfyCleYTvcYV1emmlIHHuZYx6e2m\|LDDJR\|UxKD1iMD6xNFI{OyEQvF2u	MlewQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NUK0NVIoRjJ{NEWyOFEzRC:jPh?=
mouse lymph node cells	NWP6T3RDUW2vdX7vd5VxeHKnc4PpeoUh[XO\|YYm=		MYW3NkBpenN?	M1HTXGludXWwb4P1dJBz\XO\|aY\lJIFkfGm4aYT5JIlvKGGwdHmtR2Q{N2GwdHmtR2Q5KGOxLYP0bY12dGG2ZXSgRoFt[i:lIH3veZNmKGy7bYDoJI5w\GViY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JGVNUVODLDDJR\|UxKD1iMD6wO{DPxE1w	NGfucJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS5OFYyPid-MkK0PVQ3OTZ:L3G+
mouse lymph node cells	NX;VenViS3m2b4TvfIlkcXS7IHHzd4F6		MUG0PEBpenN?	MkC5R5l1d3SxeHnjbZR6KGGpYXnud5QhSmGuYj;jJI1wfXOnIHz5cZBpKG6xZHWgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDFyLkGg{txONg>?	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR7NE[xOkc,OjJ2OUS2NVY9N2F-
HEK293	NGT3OGVHfW6ldHnvckBie3OjeR?=		NUHZUXZ1PSCvaX7z	MYfJcohq[mm2aX;uJI9nKGi3bXHuJIxqfmW{IF;BWHAySjNiZYjwdoV{e2WmIHnuJGhGUzJ7MzDGcJAuUW5iY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHs{UF2HMUetZoV1[UdidYD0ZYtmKGmwY4XiZZRm\CCob4KgOUBucW6\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFvpJF0hOS5{IN88UU4>	M4K5XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	MnK0SpVv[3Srb36gZZN{[Xl?			NXu0OHhmUW6qaXLpeIlwdiCxZjDoeY1idiCuaY\ldkBQSVSSMVKxJIV5eHKnc4Pl[EBqdiCKRVuyPVMhTmyyLVnuJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCHMUetZoV1[UdidYD0ZYtmKGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurIE2gNU4{KM7:TT6=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC2PEc,OjJ3NEGwOlg9N2F-
HEK293	NXHscpBZTnWwY4Tpc44h[XO\|YYm=		NGXZeYI2KG2rboO=	NIrtNpZKdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFBzQkOg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FNVcu[mW2YVegeZB1[WunIHnuZ5Vj[XSnZDDmc5IhPSCvaX7zJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGlEPTBiPTCxMlMh\|ryPLh?=	M3PvVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	NV\2dFlpTnWwY4Tpc44h[XO\|YYm=			NVLDcYNWUW6qaXLpeIlwdiCxZjDoeY1idiCuaY\ldkBQSVSSMVKxJIV5eHKnc4Pl[EBqdiCKRVuyPVMhTmyyLVnuJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCHMUetZoV1[UdidYD0ZYtmKGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUAyNjRizszNMi=>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC2PEc,OjJ3NEGwOlg9N2F-
HEK293	MVnGeY5kfGmxbjDhd5NigQ>?		M4XLblUhdWmwcx?=	NVT5SYl3UW6qaXLpeIlwdiCxZjDoeY1idiCuaY\ldkBQSVSSMlKxJIV5eHKnc4Pl[EBqdiCKRVuyPVMhTmyyLVnuJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCdM1jdSVNUKHWydHHr[UBqdmO3YnH0[YQh\m:{IEWgcYlveyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaTC9JFM3KM7:TT6=	NVXpSZRSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFExPjhpPkKyOVQyODZ6PD;hQi=>
HEK293	NYTiVVIyTnWwY4Tpc44h[XO\|YYm=		MVO1JI1qdnN?	NGLXTFNKdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFB{QkGg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FN3MhfXC2YXvlJIlv[3WkYYTl[EBnd3JiNTDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUA{PyEQvF2u	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC2PEc,OjJ3NEGwOlg9N2F-
T-cells	M{nHTmludXWwb4P1dJBz\XO\|aY\lJIF{e2G7		NGLlTGozPCCqcoO=	NYTNdWxNUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQlHMRk9kKG2xdYPlJHQh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDDc45CNWmwZIXj[YQhXCClZXzsJJBzd2yrZnXyZZRqd25iaX7jeYJifGWmIEK0JIhzeyCyb4P0JGNwdkFvaX7keYN1cW:wIH\vdkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOi5zMjFOwG0v	M3T0SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{K3N\|Y6Lz5{MkeyO\|M3QTxxYU6=
2008	Mn\qSpVv[3Srb36gZZN{[Xl?	NYnhXXp4OTBidV2=	NWHpc5k2OzBibXnudy=>	MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiZYjwdoV{e2WmIHnuJFIxODhiY3XscJMh[XRiMUCgeW0heHKnaX7jeYJifGWmIH\vdkA{OCCvaX7zJJBzcW:{IITvJINidGOnaX6gRW0h[WSmaYTpc44hdWWjc4Xy[YQh\X[ncomgOlAhe2WlczD1dEB1dyB7MDDtbY5{KGK7IHPhcINmcW5iQV2gZYNkfW23bHH0bY9vKGG\|c3H5Mi=>	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzBzN{i4PEc,OjNyMUe4PFg9N2F-
A2780adr	MYrGeY5kfGmxbjDhd5NigQ>?	M2DWRlExKHWP	NUTqXIJ1OzBibXnudy=>	NYTlcYpMUW6qaXLpeIlwdiCxZjDoeY1idiCSLXfwJI93\XKneIDy[ZN{\WRiaX6g[I95d3K3YnnjbY4hemW\|aYP0ZY51KEF{N{iwZYRzKGOnbHzzJIF1KDFyIIXNJJBz\WmwY4XiZZRm\CCob4KgN\|AhdWmwczDwdolweiC2bzDjZYxk\WmwIFHNJIFl\Gm2aX;uJI1m[XO3cnXkJIV3\XK7IE[wJJNm[3NidYCgeI8hQTBibXnud{BjgSClYXzj[YlvKEGPIHHjZ5VufWyjdHnvckBie3OjeT6=	MkLRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUe4PFgoRjJ\|MEG3PFg5RC:jPh?=
HL60	NIP1V\|FHfW6ldHnvckBie3OjeR?=	NIHGbXY2KHWP	M3nGWFQxKG2rboO=	MlPqTY5pcWKrdHnvckBw\iCFYUKrMYlv\HWlZXSgcYl1d2Oqb37kdolidCCyZYLt[YFjcWyrdImgeJJidnOrdHnvckBqdiCqdX3hckBJVDZyIHPlcIx{KGG2IEWgeW0h[W[2ZYKgOFAhdWmwczDy[YxifGm4ZTD0c{Bkd262cn;sMi=>	NIrMW2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyOlA5Oyd-MkOwNlYxQDN:L3G+
RPMI1788	NGP6bo9KdW23bn;zeZBxemW\|c3n2[UBie3OjeR?=		NUfGXXRMPiCmYYnz	NGX2XFJKdW23bn;zeZBxemW\|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckBTWE2LMUe4PEBk\WyuczDjc4N2dHS3cnXkJJdqfGhiaIXtZY4hWEKPQzDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGuzTH11cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjDpcoN2[mG2ZXSg[o9zKDZiZHH5d{BxemmxcjD0c{BcO0ifdHj5cYllcW6nIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDF6IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygTWM2OCB;IECuNFU1KM7:TT6=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2N{iwOEc,OjN\|NEe4NFQ9N2F-
MT4	M{DxfmFvfGm4aYLhcEBie3OjeR?=		M1TRfFQh\GG7cx?=	M3\SOmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJINwcW6oZXP0[YQhf2m2aDDIWGxXOSCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{dYOtbY5lfWOnZDDjfZRweGG2aH;n[Y5q[2m2eTDh[pRmeiB2IHThfZMh[nliTWTUJIF{e2G7LDDFR\|UxKD1iMD60OUDPxE1w	MoTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEm4PFAoRjJ\|OES5PFgxRC:jPh?=
A2780Adr	NXrrUFB7TnWwY4Tpc44h[XO\|YYm=		M3Lwb\|MxKG2rboO=	MVnJcohq[mm2aX;uJI9nKHBvZ3z5Z49xem:2ZXnuJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKGi3bXHuJGEzPzhyQXTyJINmdGy\|IHHzd4V{e2WmIHHzJINidGOnaX6tRW0h[WOldX31cIF1cW:wIIDy[Ylv[3WkYYTl[EBnd3JiM{CgcYlveyCkZX\vdoUh[2GuY3Xpck1CVSCjZHTpeIlwdiCvZXHzeZJm\CC3cDD0c{A6OCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwOUmg{txONg>?	NEXzOVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1NVEyPCd-MkO4OVEyOTR:L3G+
2008	NHTkN4tHfW6ldHnvckBie3OjeR?=		M37lO\|MxKG2rboO=	MVPJcohq[mm2aX;uJI9nKE2UUEGgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO\|ZXSgbY4hcHWvYX6gNlAxQCClZXzsd{Bie3Onc4Pl[EBieyClYXzj[YlvNUGPIHHjZ5VufWyjdHnvckBxemWrbnP1ZoF1\WRiZn;yJFMxKG2rboOgZoVnd3KnIHPhcINmcW5vQV2gZYRlcXSrb36gcYVie3W{ZXSgeZAhfG9iOUCgcYlveyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTCyMlgyKM7:TT6=	NIXoOIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1NVEyPCd-MkO4OVEyOTR:L3G+
Jurkat T	MmWySpVv[3Srb36gZZN{[Xl?		MkPDN\|AhdWmwcx?=	MWPJcohq[mm2aX;uJI9nKE6IQWSgZYN1cX[jdHnvckBqdiCSTVGvbY9vd227Y3nuMZN1cW23bHH0[YQhcHWvYX6gTpVzc2G2IGSgZ4VtdHNiaX7jeYJifGWmIH\vdkA{OCCvaX7zJJBzcW:{IITvJHBOSS:rb37vcZlkcW5ic4TpcZVt[XSrb36gcYVie3W{ZXSgZYZ1\XJiNTDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBKSzVyIE2gNE4xODF2IN88UU4>	NEHEdIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2OFk6OSd-MkO5OlQ6QTF:L3G+
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MDA435/LCC6MDR	MnvGR5l1d3SxeHnjbZR6KGG\|c3H5		NWn5cYVQOyCmYYnz	Mn\QR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR\|ZOTFJiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOyCmYYnzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDhwMzFOwG0v	NYK2OJBERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|E1PzhpPkK0NVcyPDd6PD;hQi=>
L929	MmLsR5l1d3SxeHnjbZR6KGG\|c3H5		M3L2dlMh\GG7cx?=	M1HJO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGw6OjliY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOyCmYYnzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDN\|Lkmg{txONg>?	NYjkb5NTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|E1PzhpPkK0NVcyPDd6PD;hQi=>
A2780adr	MXLGeY5kfGmxbjDhd5NigQ>?		NEnwWlV2eCC2bzC5NEBucW6\|	NF:x[lNKdmirYnn0bY9vKG:oIF3EVlEhcW5iaIXtZY4h\G:6b4L1ZolkcW5vcnXzbZN1[W62IFGyO\|gx[WS{IHPlcIx{KGG\|c3Xzd4VlKGG\|IFPhcINmcW5iQV2gZYNkfW23bHH0bY9vKHS{ZXH0[YQhOzBibXnud{Bj\W[xcnWgR4Ft[2WrbjDBUUBi\GSrdHnvckBu\WG\|dYLl[EB2eCC2bzC5NEBucW6\|IHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvQTJizszNMi=>	NUPnNJU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPFQzOTNpPkK0NVg1OjF\|PD;hQi=>
2008	M1L2TGZ2dmO2aX;uJIF{e2G7		NWXkd291fXBidH:gPVAhdWmwcx?=	NF;zSGhKdmirYnn0bY9vKG:oIF3SVFEhcW5iaIXtZY4hOjByODDj[YxteyCjc4Pld5Nm\CCjczDDZYxk\WmwIFHNJIFk[3WvdXzheIlwdiC2cnXheIVlKDNyIH3pcpMh[mWob4LlJGNidGOnaX6gRW0h[WSmaYTpc44hdWWjc4Xy[YQhfXBidH:gPVAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzNjdizszNMi=>	MofWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOESyNVMoRjJ2MUi0NlE{RC:jPh?=
Saos2	NGn2cldHfW6ldHnvckBie3OjeR?=	NH23flQ2KHWP	MnjCN{BpenN?	MlW2TY5pcWKrdHnvckBw\iCyLXfwJIlvKGSxeH;yeYJq[2mwLYLld4l{fGGwdDDoeY1idiCVYX;zNkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCmb4jvdpVjcWOrbjD1dJRic2ViYYSgOUB2VSCjZoTldkA{KGi{czDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm\|aYOu	NHHWTY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEeyNFQ2Oid-MkS3NlA1PTJ:L3G+
Saos2	NUnSTlF7TnWwY4Tpc44h[XO\|YYm=	M{G2VVUhfU1?	M16xbFIhcHK\|	M3r1NWlvcGmkaYTpc44hd2ZicD3ndEBqdiCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iU3Hvd\|Ih[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBld3ixcoXibYNqdiC{ZYTlcpRqd25iYYSgOUB2VSCjZoTldkAzKGi{czDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm\|aYOu	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{MES1Nkc,OjR5MkC0OVI9N2F-
Saos2	M1O4S2Z2dmO2aX;uJIF{e2G7	MXq1JJVO	NGqwU5MzKHSxIEOgbJJ{	MX3Jcohq[mm2aX;uJI9nKHBvZ4CgbY4h\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKFOjb4OyJINmdGy\|IHHzd4V{e2WmIHHzJIlvfHKjY3XscJVt[XJiZH;4c5J2[mmlaX6g[Il{fHKrYoX0bY9vKGmwIH71Z4xmcSCjdDC1JJVOKGGodHXyJFIhfG9iMzDodpMh[nlibHHz[ZIhe2Ojbn7pcoch[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXNw	NFzRe5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEeyNFQ2Oid-MkS3NlA1PTJ:L3G+
mouse	Ml\QSpVv[3Srb36gZZN{[Xl?			MVHJcohq[mm2aX;uJI9nKGi3bXHuJG1FWjFiZYjwdoV{e2WmIHnuJI1wfXOnIFy1NVc5KGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh[WOldX31cIF1cW:wIH;mJJJpd2SjbXnu[UAyOjNiYomgSmFEW0OjbHnieZIh\myxdzDjfZRwdWW2comsJGlEPTBiPTCwMlY4KM7:TT6=	M4PQUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwNlI3Lz5{NEmwNFIzPjxxYU6=
A2780	NYDZfVQ{TnWwY4Tpc44h[XO\|YYm=	NXTw[HhXPSC3TR?=	NV[0TmhsPDBibXnudy=>	MknXTY5pcWKrdHnvckBw\iCSZ4CtcYVlcWG2ZXSg[JJ2\yCnZn\seZghcW5iYXTybYFugWOrbj3y[ZNqe3SjboSgbJVu[W5iQUK3PFAh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBi\HKrYX35Z4lvKGyndnXsJIF1KDVidV2gZYZ1\XJiNECgcYlveyCkeTDmcI94KGO7dH;t[ZRzgSCjbnHsfZNqey5?	M3niRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwN\|I{Lz5{NEmwNFMzOzxxYU6=
A2780	MWrGeY5kfGmxbjDhd5NigQ>?	MVy1JJVO	Ml3qNUBpeg>?	NXzEU5RCUW6qaXLpeIlwdiCxZjDQ[5AudWWmaXH0[YQh\HK3ZzDl[oZtfXhiaX6gZYRzcWGveXPpck1z\XOrc4ThcpQhcHWvYX6gRVI4QDBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYRzcWGveXPpckB2eHSja3WgZZQhPSC3TTDh[pRmeiBzIHjyJIJ6KG[ub4egZ5l1d22ndIL5JIFv[Wy7c3nzMi=>	M1O5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwN\|I{Lz5{NEmwNFMzOzxxYU6=
A2780	MW\GeY5kfGmxbjDhd5NigQ>?	MVS1JJVO	MmH3PEBpenN?	M2LxfmlvcGmkaYTpc44hd2ZiUHfwMY1m\GmjdHXkJIRzfWdiZX\mcJV5KGmwIHHkdolidXmlaX6tdoV{cXO2YX70JIh2dWGwIFGyO\|gxKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHHkdolidXmlaX6geZB1[WunIHH0JFUhfU1iYX\0[ZIhQCCqcoOgZpkh\myxdzDjfZRwdWW2comgZY5idHm\|aYOu	MonDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECzNlMoRjJ2OUCwN\|I{RC:jPh?=
T-cells	MUnBcpRqcW6obHHtcYF1d3K7IHHzd4F6	M4DWUFAvODFidH:gNVAxKG6P	M3[ycVIh\GG7cx?=	MkfyRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBNVE9zMUigWGNTKHS{YX7z[4VvcWNibX;1d4UhUUxvMj3jc41x\XSnboSgR2Q1NXCxc3n0bZZmKFRiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNVE9iKEG5NEB1dyB{MEWpMYlv\HWlZXSgTWwuOiCycn;keYN1cW:wIHH0JFAvODFidH:gNVAxKG6PIH\vdkAzKGSjeYOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMi=>	NIPzVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFg3Pid-MkS5NFA5PjZ:L3G+
Th17	NVjW[o5pSW62aXnu[oxidW2jdH;yfUBie3OjeR?=	NF\3T3cyOCC2bzCxNFAhdk1?	Mof5NkBl[Xm\|	NYLzPVhtSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCOTF:xNVghXEOUIITyZY5{\2WwaXOgcY92e2ViVHixO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFnMMVE4KHC{b3T1Z5Rqd25iYYSgNVAhfG9iMUCwJI5OKG[xcjCyJIRigXNiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m\|Lh?=	M2[wVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwPFY3Lz5{NEmwNFg3PjxxYU6=
Th1	MUDBcpRqcW6obHHtcYF1d3K7IHHzd4F6	NH7Yd5gyODBibl2=	NXzRWlFuOiCmYYnz	Mnq0RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBNVE9zMUigWGNTKHS{YX7z[4VvcWNibX;1d4UhXGhzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhUU[QLXfhcY1iKHC{b3T1Z5Rqd25iYYSgNVAxKG6PIH\vdkAzKGSjeYOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMi=>	NXK0eW9pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA5PjZpPkK0PVAxQDZ4PD;hQi=>
NCI/ADR-RES	MWnGeY5kfGmxbjDhd5NigQ>?		NXvXc\|FQOjBibXnudy=>	NXXLTZB[UW6qaXLpeIlwdiCxZjDoeY1idiCSLXfsfYNweHKxdHXpckBmgHC{ZYPz[YQhcW5iTlPJM2FFWi2URWOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKGOjbHPlbY4uSU1idILhcpNxd3K2IHHmeIVzKDJyIH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7Lh?=	NGLsZlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5NlE2Oyd-MkS5PVIyPTN:L3G+
A2780adr	MkfYSpVv[3Srb36gZZN{[Xl?		Ml;BPVAhdWmwcx?=	M1PzfWlvcGmkaYTpc44hd2ZiQVLDRlEhcW5iaIXtZY4hSTJ5OEDh[JIh[2WubIOgdJJmNWmwY4XiZZRm\CCob4KgN\|AhdWmwczDmc4xtd3enZDDifUBk[WylZXnuJGFOKGGmZHn0bY9vKGGwZDDmeZJ1cGW{IHnuZ5Vj[XSnZDDmc5IhPjBibXnud{BjgSClYXzj[YlvKEGPIHHzd4F6NCCLQ{WwJF0hOC57MUmg{txONg>?	NVj2[plkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OVU5QTVpPkK1PFU2QDl3PD;hQi=>
T spleen cells	NHPtb5ZEgXSxdH;4bYNqfHliYYPzZZk>		MmXnO\|IhcHK\|	NEXhS3JEgXSxdH;4bYNqfHliYXfhbY5{fCCLQ2KgcY92e2ViVDDzdIxm\W5iY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODFizszNMi=>	NYHTOmNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlQzPjZpPkK2OVY1OjZ4PD;hQi=>
B spleen cells	MWrDfZRwfG:6aXPpeJkh[XO\|YYm=		NInHTZY4OiCqcoO=	MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBKS1JibX;1d4UhSiC\|cHzl[Y4h[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFQh\|ryPLh?=	NWfUTnRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlQzPjZpPkK2OVY1OjZ4PD;hQi=>
RD	NX;4TolRSW62aY\pdoFtKGG\|c3H5		NIXiOpkzPCCqcoO=	M1HSbmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGVXPzFiaX7m[YN1\WRiaX6gVmQh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSB\|Lk[2JO69VS5?	NXrWfW9[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlQzPjZpPkK2OVY1OjZ4PD;hQi=>
mouse lymph node cells	MYHGeY5kfGmxbjDhd5NigQ>?			NWj3d\|k3UW6qaXLpeIlwdiCxZjDQTVNMNWejbX3hJIlvKG2xdYPlJIx6dXCqIH7v[IUh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDDc45CNXO2aX31cIF1\WRiVDDj[YxtKHC{b3zp[oVz[XSrb36sJGlEPTBiPTCwMlU4KM7:TT6=	M1H6bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{KwNVU1Lz5{NkeyNFE2PDxxYU6=
CD1	NIG2V2NHfW6ldHnvckBie3OjeR?=	NF;xe2IxNjFidH:gNVAhfU1?	NGLsXpQ{OCCvaX7z	NUntfoRVUW6qaXLpeIlwdiCxZjD0ZZVzd2yrdHjvZ4hwdGG2ZTDhZ4llKDNvc4Xs[oF1\S2rbnT1Z4VlKG6nY4Lvd4l{KGmwIFPENUBud3W\|ZTDwZY5kemWjdHnjJIFkcW6jcjDj[YxteyCjdDCwMlEhfG9iMUCgeW0h[W[2ZYKgN\|AhdWmwczDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJie2WmIHPvcoZw[2GuIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzMi=>	M1OyN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUWwN\|kzLz5{Nkm1NFM6OjxxYU6=
A2780/ADR	NV62XmR3TnWwY4Tpc44h[XO\|YYm=		MXGzNEBucW6\|	NVnwbZlJUW6qaXLpeIlwdiCxZjDBRmNDOSCrbjDoeY1idiCDMke4NE9CTFJiY3XscJMhcW6ldXLheIVlKG[xcjCzNEBucW6\|IH3lZZN2emWmIIXwJJRwKDN4MECgd4VkeyC5aYToJJRqdWViaX70[ZJ3[Wy\|IH;mJFYxKHOnY4OgZpkh[2GuY3XpckBi[2O3bYXsZZRqd25iYYPzZZktKEmFNUCgQUAyNjRzIN88UU4>	NXLQ[mNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNFAxOzNpPkK3NVAxODN\|PD;hQi=>
A2780/ADR	NFrEWWpEgXSxdH;4bYNqfHliYYPzZZk>		Mlu1O\|IhcHK\|	MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OC:DRGKgZ4VtdHNib4\ldoV5eHKnc4PpcochSUKFQkGgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEeLNUCgQUA5NjZ\|IN88UU4>	NEjaUJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GwNFA{Oyd-MkexNFAxOzN:L3G+
A2780	NVrONWs3S3m2b4TvfIlkcXS7IHHzd4F6		NVHYdJRFPzJiaILz	NXztd4NQS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGdKPTBiPTC4Mlk1KM7:TT6=	Mn65QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMECwN\|MoRjJ5MUCwNFM{RC:jPh?=
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Flp-InTM-293	NWj5RoM3TnWwY4Tpc44h[XO\|YYm=	NX3oZVlEOjVidV2=	NGTPXm8{OCCvaX7z	MnS1TY5pcWKrdHnvckBw\iCDQlPDNkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBHdHBvSX7UUU0zQTNiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk[WylZXnuMWFOKGWoZnz1fEBifCB{NTD1UUBu\WG\|dYLl[EBi\nSncjCzNEBucW6\|IHnuJJBz\XOnbnPlJI9nKEGEQ1KxJIlvcGmkaYTvdkBITjF{MEmxPEBidmRiQVLDR\|EhcW6qaXLpeI9zKE2NLUW3NUBjgSCobH;3JIN6fG:vZYTyfU4>	NGHhO5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5N\|k1QSd-MkezPVM6PDl:L3G+
hamster BHK21	NFvYb5pHfW6ldHnvckBie3OjeR?=	Mn;iNlUhfU1?	MmXpN\|AhdWmwcx?=	NFWxT\|RKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1OxJIV5eHKnc4Pl[EBqdiCqYX3zeIVzKEKKS{KxJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3HsZ4Vqdi2DTTDl[oZtfXhiYYSgNlUhfU1ibXXhd5Vz\WRiYX\0[ZIhOzBibXnud{BjgSCobH;3JIN6fG:vZYTyfU4>	NVLzSGtrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezPVM6PDlpPkK3N\|k{QTR7PD;hQi=>
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Jurkat T	MVTJcY12dm:\|dYDwdoV{e2m4ZTDhd5NigQ>?		NY\j[5pqOTNiaILz	MXfJcY12dm:\|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBpfW2jbjDQUWEwcW:wb335Z4lvKHO2aX31cIF1\WRiSoXyb4F1KFRiY3XscJMh[XO\|ZYPz[YQh[XNic4XwdJJme3Orb36gc4YhUUx{IIDyc4R2[3Srb36gdJJmfHKnYYTl[EB4cXSqIHPlcIx{KG[xbHzve4VlKGK7IGDNRU9qd26xbYnjbY4he3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOTNiaILzJIJ6KEWOSWPBMEBKSzVyIE2gNE4xOTFizszNMi=>	M{LlRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N\|cxLz5{N{[0O\|M4ODxxYU6=
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replicon 2A cells	MXnGeY5kfGmxbjDhd5NigQ>?		M1vLZVczKGi{cx?=	MUHJcohq[mm2aX;uJI9nKG2WT2Kgd4lodmGuaX7nJJBifGi5YYmgbY4hMHWwa37ve44hd3KrZ3nuLUBqdiC\|dXKt[4Vvd22rYzDy[ZBtcWOxbjCyRUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5icHjvd5Bpd3K7bHH0[YQhenC\|NjDwdo91\WmwIHzleoVteyC3cDD0c{A1ODBibl2gZYZ1\XJiN{KgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpd{4>	MkC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NEKyOVYoRjJ6NESyNlU3RC:jPh?=
A2780adr	NWq2O\|FbTnWwY4Tpc44h[XO\|YYm=		MkG1N\|AhdWmwcx?=	NYTWTo51UW6qaXLpeIlwdiCxZjDBRmNDOSCrbjDoeY1idiCDMke4NIFleiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4Ft[2WrbjDBUUBt\X[nbIOgdJJmcW6ldXLheIVlKG[xcjCzNEBucW6\|IH\vcIxwf2WmIHL5JINidGOnaX6gRW0h[WSmaYTpc44hdWWjc4Xy[YQhcW2vZXTpZZRmdHliYYSgOlAhe2WlIITpcYUhcW62ZYL2ZYwh\m:{IE[wJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBKSzVyIE2gNU4zOSEQvF2u	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR5MU[1Okc,Ojh2N{G2OVY9N2F-
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H69AR	Ml73SpVv[3Srb36gZZN{[Xl?		NVfBfmZ6OzBibXnudy=>	MYjJcohq[mm2aX;uJI9nKEGEQ1OxJIlvKGi3bXHuJGg3QUGUIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk[WylZXnuMWFOKGWoZnz1fEBxemWrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkh[2GuY3Xpck1CVSCjZHTpeIlwdiCvZXHzeZJm\CCjdDC2NEB{\WO\|IHnueIVzfmGuIH\vdkA3OCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDNwNUOg{txONg>?	NX71ZZJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4OFE2OTNpPkK4PFQyPTF\|PD;hQi=>
A673	Mn7TdWhVWyCjc4PhfS=>			M2Xhd5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy\|Lh?=	NYn3N2N4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2	MW\xTHRUKGG\|c3H5			MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy\|Lh?=	M1u3flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NB1643	MmX3dWhVWyCjc4PhfS=>			NYfaN2Q5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsd{4>	M4HERlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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Rh41	MYHxTHRUKGG\|c3H5			M3LSfJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJMv	MkDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	NIXVNnpyUFSVIHHzd4F6			Ml[5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIOu	M2GxWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18	MVTxTHRUKGG\|c3H5			NEj6SnlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucz6=	NGf0SII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-MC	M4HCd5FJXFNiYYPzZZk>			MoS5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtey5?	NEjmZZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
TC32	MmD1dWhVWyCjc4PhfS=>			NUDESVRmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtey5?	NUnzTVBvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
C57BL/6 mouse lymph node cells	MWrJcY12dm:\|dYDwdoV{e2m4ZTDhd5NigQ>?		MXuyOEBpenN?	NVyyWWNVUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5iQ{W3RmwwPiCvb4Xz[UBtgW2yaDDuc4RmKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCwMlUh\|ryPLh?=	M1;IVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEexPFQ1Lz5{OUi3NVg1PDxxYU6=
HuH7	Moe1RY51cX[rcnHsJIF{e2G7		MnnWN{Bl[Xm\|	NVnBOHUxSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIHflco91gXCnIELhJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[W[2ZYKgN{Bl[Xm\|IHL5JIR2[WxvZ3zvJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwN{ezJO69VS5?	MnPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByN{S3PVUoRjNyMEe0O\|k2RC:jPh?=
MT4	NYHidY5rS3m2b4TvfIlkcXS7IHHzd4F6		MVG0JJRwKDViZHH5dy=>	M136[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1VPCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPCC2bzC1JIRigXNiYomgZ4VtdC22aYTldkBodG9iYYPzZZktKEOFNUCgQUA2NjZyODFOwG0v	M{Xvb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEe0O\|k2Lz5\|MEC3OFc6PTxxYU6=

... Click to View More Cell Line Experimental Data

体内研究

Cyclosporin A是一种免疫抑制剂，小鼠，大鼠，和荷兰猪经非肠道给药或者口服给药都有效^[6]，临床上用于器官移植中预防免疫排斥。^[7]

激酶实验：^[3]	- 合并磷酸酶试验: 纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶，100 nM 钙调蛋白，以及5 μM ³²P-标记磷酸化多肽，60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8)，100 mM NaCI，6 mM MgCl₂，0.5 mM 二硫苏糖醇，每毫升0.1毫克牛血清白蛋白，以及0.1 mM CaCl₂或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物， 5 μM ³²P-标记的磷酸肽，和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸，一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca²⁺-不依赖性磷酸酶，而更高浓度时也会部分抑制Ca²⁺-依赖性的活性。30°C下培育15分钟，加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐，用闪烁计数测定数量。
细胞实验：^[3]	- 合并 Cell lines: Jurkat细胞(无性繁殖J77) Concentrations: ~100 nM Incubation Time: 1小时 Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(10⁶)悬浮于微量离心管1ml完全培养基中；1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中，细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次，在50μl低渗缓冲液中裂解，缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯，50 微克胰蛋白酶抑制剂，5微克亮抑酶肽，和5 微克抑肽酶。裂解液用液氮冷冻，再在30°C下融化，循环3次，随后在 4°C下以12,000×g离心10分钟。 (Only for Reference)
动物实验：^[6]	- 合并 Animal Models: 大鼠 Dosages: 45毫克/千克 Administration: 口服 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	100 mg/mL warmed (83.15 mM)
	Ethanol	100 mg/mL warmed (83.15 mM)
	Water	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+5% Tween 80+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Cyclosporin A SDF

分子量	1202.61
化学式	C₆₂H₁₁₁N₁₁O₁₂
CAS号	59865-13-3
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	Cyclosporine A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04023760	Completed	Drug: Apixaban	Pharmacokinetics\|Kidney Transplant\|Lung Transplant	University of Saskatchewan\|Saskatchewan Health Research Foundation\|Lung Association of Saskatchewan	June 26 2019	Phase 4
NCT03865888	Completed	Drug: Cyclosporins\|Drug: Tacrolimus	Dry Eye\|Sjogren Syndrome	Pavly Moawd\|Cairo University	October 30 2018	Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?
回答：
Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

研究领域

产品类型

定制您的化合物库

Cyclosporin A

客户使用Selleck生产的Cyclosporin A 发表文献40篇：

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Cyclosporin A SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2020-01-06)

技术支持

常见问题及建议解决方法