Cyclosporin A (NSC 290193)

For research use only. Not for use in humans.

目录号：S2286 别名： Cyclosporine A, Cyclosporine, Ciclosporin, CsA 中文名称：环孢菌素A

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂，与亲环素结合，随后抑制calcineurin，无细胞试验中IC50为7 nM，广泛用于预防器官移植反应中的排斥反应。

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库存

购买数量

10mM (1mL in DMSO)	RMB 1073.15	现货
50mg	RMB 786.07	现货
200mg	RMB 1957.41	现货
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客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献54篇：

Cancer Cell, 2018, 33(3):480-494

PubMed: 29533786 ( click the link to review the publication )

Cell Rep, 2021, 35(7):109159

PubMed: 34010655 ( click the link to review the publication )

PLoS Biol, 2021, 19(4):e3001134

PubMed: 33901180 ( click the link to review the publication )

Int J Biol Sci, 2021, 17(13):3672-3688

PubMed: 34512174 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(14)7540

PubMed: 34299155 ( click the link to review the publication )

Exp Neurol, 2021, 338:113598

PubMed: 33422553 ( click the link to review the publication )

J Cell Mol Med, 2021, 25(12):5729-5743

PubMed: 33949118 ( click the link to review the publication )

Front Pharmacol, 2021, 12:706901

PubMed: 34483914 ( click the link to review the publication )

Phytomedicine, 2021, 85:153551

PubMed: 33827043 ( click the link to review the publication )

Br J Nutr, 2021, 1-11

PubMed: 33663624 ( click the link to review the publication )

Peptides, 2021, S0196-9781(21)00117-0

PubMed: 34242679 ( click the link to review the publication )

Clin Transl Med, 2021, 11(4):e390

PubMed: 33931967 ( click the link to review the publication )

Mol Cell, 2021, S1097-2765(21)00087-3

PubMed: 33626321 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2021, 414:115426

PubMed: 33524445 ( click the link to review the publication )

Anal Chem, 2020, 10.1021/acs.analchem.0c02756

PubMed: 32933243 ( click the link to review the publication )

Free Radic Biol Med, 2020, 150:109-119

PubMed: 32105828 ( click the link to review the publication )

Toxicol In Vitro, 2020, 8;66:104857

PubMed: 32278034 ( click the link to review the publication )

J Fungi (Basel), 2020, 6(3):E103

PubMed: 32650564 ( click the link to review the publication )

Circulation, 2019, 10.1161/CIRCULATIONAHA.118.037227

PubMed: 31163979 ( click the link to review the publication )

J Clin Invest, 2019, 129(6):2431-2445

PubMed: 31081803 ( click the link to review the publication )

Autophagy, 2019, 15(3):493-509

PubMed: 30304977 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-19-1982

PubMed: 31582380 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):487

PubMed: 31822296 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2019, 14:192-203

PubMed: 30623853 ( click the link to review the publication )
Front Immunol, 2019, 10:2952

PubMed: 31921197 ( click the link to review the publication )

J Antimicrob Chemother, 2019, 74(7):1921-1927

PubMed: 30934052 ( click the link to review the publication )

Oxid Med Cell Longev, 2019, 2019:4248529

PubMed: 30881590 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(10):6897-6906

PubMed: 31379115 ( click the link to review the publication )

Front Microbiol, 2019,

PubMed: 32038504 ( click the link to review the publication )

J Med Virol, 2019, 91(8):1440-1447

PubMed: 30900754 ( click the link to review the publication )

Med Sci Monit, 2019,

PubMed: 30913205 ( click the link to review the publication )

Biochim Biophys Acta Gene Regul Mech, 2019, 1862(8):846-857

PubMed: 31054961 ( click the link to review the publication )

Nat Commun, 2018, 9(1):873

PubMed: 29491374 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(23):6066-6077

PubMed: 30061362 ( click the link to review the publication )

Oncogenesis, 2018, 7(11):91

PubMed: 30467308 ( click the link to review the publication )

Sci Rep, 2018, 8(1):10910

PubMed: 30026610 ( click the link to review the publication )

Chem Res Toxicol, 2018, 31(2):127-136

PubMed: 29156121 ( click the link to review the publication )

Sci Adv, 2018, 4(8):eaas9784

PubMed: 30101190 ( click the link to review the publication )

Nat Cell Biol, 2017, 19(10):1274-1285

PubMed: 28892080 ( click the link to review the publication )

Elife, 2017, 6e26733

PubMed: 28737489 ( click the link to review the publication )

J Neurochem, 2017, 143(3):282-293

PubMed: 28902407 ( click the link to review the publication )

Int J Cardiol, 2017, 227:201-208

PubMed: 27839819 ( click the link to review the publication )

Am J Physiol Renal Physiol, 2017, 313(2):F404-F413

PubMed: 28381458 ( click the link to review the publication )

PLoS One, 2017, 12(10):e0187123

PubMed: 29073235 ( click the link to review the publication )

J Mol Neurosci, 2017, 61(2):267-278

PubMed: 28054226 ( click the link to review the publication )

Pharm Res, 2016, 33(2):395-403

PubMed: 26415646 ( click the link to review the publication )

Int J Mol Med, 2016, 37(3):631-8

PubMed: 26846204 ( click the link to review the publication )

J Virol Methods, 2016, 238:13-20

PubMed: 27678028 ( click the link to review the publication )
Oncotarget, 2016, 7(6):6711-26

PubMed: 26744319 ( click the link to review the publication )

Oncogene, 2015, 10.1038/onc.2015.32

PubMed: 25728673 ( click the link to review the publication )

Antiviral Res, 2015, 120:140-6

PubMed: 26086883 ( click the link to review the publication )

Drug Metab Dispos, 2014, 42(3):318-22

PubMed: 24335466 ( click the link to review the publication )

Arch Environ Con Tox, 2014, 68(1):31-7

PubMed: 25194241 ( click the link to review the publication )

Biochim Biophys Acta, 2013, 1833(3):652-62

PubMed: 23228564 ( click the link to review the publication )

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述

靶点

calcineurin ^[1]
(Cell-free assay)

7 nM

体外研究

在T细胞中，Cyclosporin A与亲环蛋白(免疫亲和素)结合^[1]，形成亲环蛋白-Cyclosporin A复合体，其结合并抑制钙调磷酸酶。^[2]Cyclosporin A 抑制钙调磷酸酶，IC50 是7 nM^[3]，随后阻断NF-AT核转运。 ^[4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体，IC50 是39 nM。^[5]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
rat H42E cells	MkflR5l1d3SxeHnjxsBie3OjeR?=			NUfNU4ltS3m2b4TvfIlkcXS7IHHnZYlve3RicnH0JGg1OkViY3XscJMh[XO\|ZYPz[YQh[XNiaX70doFk\WyudXzhdkBCXFBibHX2[YwtKFSFNUC9OFIh\|ryP	M2H6ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUCyNFU5Lz5zOUWwNlA2QDxxYU6=
mouse L929 cells	NGHFRYtEgXSxdH;4bYPDqGG\|c3H5		NFzE[Ww{KGSjeYO=	Mo\OR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFkzQSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRUKGG\|c3H5MEBKSzVyPUOwJO69VQ>?	MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human Huh-Mono cells	NUG5fGlDS3m2b4TvfIlkyqCjc4PhfS=>		NEHUbHY{KGSjeYO=	MoDaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNU2xbn:gZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5Nige,:jDDDR\|UxRTJ2IN88US=>	MoOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O\|Y3RC:jPh?=
MDCK cells	M3S2XWN6fG:2b4jpZ:Kh[XO\|YYm=			M{foPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQ1ugZ4VtdHNuIGTDOVA:QS57IN88US=>	MmG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells	Ml\vR5l1d3SxeHnjxsBie3OjeR?=		MXy3NkBp	MlH3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUGNENTZiY3XscJMh[W[2ZYKgO\|IhcHK\|LDDJR\|UxRThwMzFOwG0>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ\|MkC0NFI9N2F-
human HeLa cells	NEHlVXpEgXSxdH;4bYPDqGG\|c3H5			NYTVbHA1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyxiVFO1NF05NjNizszN	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
african green monkey Vero cells	NH21W21HfW6ldHnvckBie3OjeR?=		M1jqNFI1KGh?	Ml3sRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fCCQaXzlJJZqenW\|IHnu[oVkfGWmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KF[ncn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygVm5CKHO7boTo[ZNqeyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR\|UxRThizszN	MkjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGyPFYoRjF7NEWxNlg3RC:jPh?=
human Huh-9-13 cells	MWLDfZRwfG:6aXRCpIF{e2G7		NXfQcYJqOyCmYYnz	NXPwbpVtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqLUmtNVMh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3Ojef-8kEBESzVyPUeg{txO	MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O\|Y3RC:jPh?=
human HuH6 cells	MmLuR5l1d3SxeHnjxsBie3OjeR?=		M3\heVMh\GG7cx?=	NIXnPIhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh4IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEOFNUC9O{DPxE1?	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human Huh5-2 cells	Mo\oR5l1d3SxeHnjxsBie3OjeR?=		NHjUd3I{KGSjeYO=	MnHBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPS1{IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZkhNCCFQ{WwQVYh\|ryP	M{jsV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O\|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH7 cells	MofzR5l1d3SxeHnjxsBie3OjeR?=			NWDJNVRRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[Yxm[XOnIH;mJIxi[3SjdHWg[IVpgWS{b3flcoF{\SxiSVO1NF02NjhzIN88US=>	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl2M{O5NEc,OjB7NEOzPVA9N2F-
human 2008 cells	M4jrTmZ2dmO2aX;uJIF{e2G7			M2S4UGlvcGmkaYTpc44hd2ZiaIXtZY4hVVKSMTDpckBpfW2jbjCyNFA5KGOnbHzzMEBKSzVyPUSuO\|gh\|ryP	NGLtbJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEewO\|g5PCd-MUi3NFc5QDR:L3G+
human HeLa cells	M3fRZWZ2dmO2aX;uJIF{e2G7			NITUSoVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJWFZvMUigbY5n\WO2ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEeg[IF6e+,:jDDUR\|UxRTRwNjFOwG0>	NHrW[JM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
leukemia CEM cells	MofxSpVv[3Srb36gZZN{[Xl?			NV\Jd5FTUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEi3bXHuJG1FWjFiUD3HcJlkd3C{b4TlbY4hSUKFIGTyZY5{eG:{dHXyJJV{cW6pIHzleYtmdWmjIFPFUUBk\WyuczygTWM2OD1\|LkSg{txO	M3\4UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN\|g4Lz5zMkO2NVM5PzxxYU6=
human Caco2 cells	NELTN2tHfW6ldHnvckBie3OjeR?=			M{nPOY5pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJGNi[29{IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBlcWexeHnuJIVn\my3eDygTWM2OD1{Lkmg{txO	MkDPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|OEK0OVgoRjJ\|M{iyOFU5RC:jPh?=
human MDA435/LCC6MDR cells	NEPqSpdEgXSxdH;4bYPDqGG\|c3H5		MWG3Nog>	Mn3kR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR\|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=>	M1zZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
guinea pig ventricular myocytes	Mne2SpVv[3Srb36gZZN{[Xl?			M2raSWlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG\|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1?	NIrySYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2NVAxOCd-MkK3OlExODB:L3G+
KB/MDR cell	MofESpVv[3Srb36gZZN{[Xl?		M3LUcVczKGh?	Moq5TY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDN02GUjDj[YxtKGyrbnWgZYZ1\XJiN{KgbJIhd2ZiZIL1[{BmgHCxc4Xy[UwhUUN3ME2xMlUh\|ryP	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MUm5NUc,OTV2OEG5PVE9N2F-
A2780/ADR cells	MnLDSpVv[3Srb36gZZN{[Xl?			M1T6S2lvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4h\XiycnXzd4VlKGmwIFGyO\|gxN0GGUjDj[YxteyCkeTDjZYxk\WmwIFHNJIF{e2G778{MJGlEPTB;MT60NVI2PCEQvF2=	NXGyc\|VTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells	NW\lSGVWTnWwY4Tpc44h[XO\|YYm=	NITVZmgyKM7:TR?=	NF76b5Y4OiCq	NXu0ZVFRWG:2ZX70bYF1cW:wIH;mJIRwgG:{dXLpZ4lvNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIwWjdiY3XscJMh[XO\|ZYPz[YQh[XNiZH;4c5J2[mmlaX6gTWM2OCCjdDCxJJVOKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE44KM7:TR?=	MnnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{SwOFEoRjJ3NkO0NFQyRC:jPh?=
LLC-PK1 epithelial cells	M1fySmZ2dmO2aX;uJIF{e2G7			NET5bWdKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNCCvb4Xz[UBNNW2mckHiJIV5eHKnc4Pl[EBqdiCOTFOtVGsyKGWyaYTo[Yxq[WxiY3XscJMhfXOrbnegZ4Ft[2Wrbj3BUUBxd2yjcnnzZZRqd25iYYPzZZnwxIxiSVO1NF0xNjdizszN	M3rRe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N\|g6Lz5zMk[5PVM5QTxxYU6=
mouse L5178 cells	NF\qfJNHfW6ldHnvckBie3OjeR?=		NV7KNHo4OjBibXnudy=>	NE[1W3FKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1KxMY1m\GmjdHXkJJJpd2SjbXnu[UAyOjNiZX\mcJV5KGmwIH3veZNmKEx3MUe4JINmdGy\|IHX4dJJme3OrbnegbJVu[W5iTVTSNUBi\nSncjCyNEBucW6\|IHL5JGZCS1NiYX7hcJl{cXNuIFnDOVA:OC54NTFOwG0>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2MUe0OUc,OjF\|NEG3OFU9N2F-
KB cell	MX3GeY5kfGmxbjDhd5NigQ>?			M3fT[2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBMSiClZXzsJIxqdmViYX\0[ZIhPzJiaDDv[kBlenWpIHX4dI9{fXKnLDDJR\|UxRTBwNjFOwG0>	M3j0S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
mouse mammary carcinoma cell line EMT6/AR 1.0	NV25NZVYTnWwY4Tpc44h[XO\|YYm=			MY\S[ZZmenOjbDDl[oZm[3Rib36geIhmKGGlY4XteYxifGmxbjDv[kBcO0ifLTDkZZVvd3K3YnnjbY4hcW5ibX;1d4UhdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mKEWPVE[vRXIhOS5yLDDJR\|UxRTBwNESg{txO	NWTOVIgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwPVg3PzFpPkGwNFk5PjdzPD;hQi=>
CK1 cells	M37hUmZ2dmO2aX;uJIF{e2G7			NUfDU2RvSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUOzJIlv\mWldHXkJIlvKEONMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFch\GG7czygWGM2OD1yLkOg{txO	M4LoNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
HEK293 cells	NF[yVWFHfW6ldHnvckBie3OjeR?=			MmnJTY5pcWKrdHnvckBw\iCRQWTQNWIyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCycn;0[YlvNW2nZHnheIVlKHCrdHH2ZZN1[XSrbjD1dJRic2YxvJygT4k:OC5{IN88US=>	MkHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OEe5PFYoRjJ{NUi3PVg3RC:jPh?=
human MDA435/LCC6MDR cells	NEP1NmJHfW6ldHnvckBie3OjeR?=		NYH5UnpVPSCmYYnz	NH7CTFlOd2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N\|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpcocheGGlbHn0ZZhmdCC{ZYPpd5RidmOnIH3lZZN2emWmIHHzJINmdGxic4Xyeol3[WxiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5NigSxiRVO1NF0xNjB\|MjFOwG0v	M{jieFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC	MkH0VJJwdGmoZYLheIlwdiCjc4PhfS=>			NFq1bHNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDCUWMtKEmFNUC9NVIhdk1?	NIjlbVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[3PVMxQSd-MUW2O\|k{ODl:L3G+
mouse T cells	M4PMTGZ2dmO2aX;uJIF{e2G7		M2DzOVQh\GG7cx?=	NWfUXnlqUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5ibX;1d4UhXCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJR4dyC5YYmgcYl5\WRibIntdIhw[3m2ZTDy[YFkfGmxbjDh[pRmeiB2IHThfZMh[nliTWTUJIF{e2G7LDDpZ\|UxRTFzIH7N	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6NEm4O{c,OjJ7OES5PFc9N2F-
human splenocytes	NF\JZm9HfW6ldHnvckBie3OjeR?=		MkjROEBl[Xm\|	MnrLTY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hcHWvYX6gd5Bt\W6xY4n0[ZMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifdHj5cYllcW6nIHnuZ49zeG:{YYTpc44h[W[2ZYKgOEBl[Xm\|IHL5JIFtdG:pZX7pZ{BucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJIF{e2G7LDDJR\|UxRTFyIH7N	M3XaNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEK3PFMyLz5zOUiyO\|g{OTxxYU6=
human T-cell	M4fjVGZ2dmO2aX;uJIF{e2G7			Mnf5TY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGStZ4VtdCCycn;keYN1cW:wIH;mJIx6dXCqb3vpcoUhUUxvMjygTWM2OD16LkGgcm0>	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84PTN5M{OxK\|44PTN5M{OxQE9iRg>?
human Jurkat cells	M2PpNGZ2dmO2aX;uJIF{e2G7		M4rUVVEhcA>?	NFG4Z41CdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKGi3bXHuJGp2emujdDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGGwdHmtR2Q{N0OGMkigZY51cWKxZImtbY5lfWOnZDDJUE0zKHC{b3T1Z5Rqd25idILlZZRm\CCob4KgNUBpeiCkZX\vdoUh[W62aT3DSFMwS0R{ODDhcpRq[m:meTDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMh[nliaX3teY5we3WycILld5Nqd25iYYPzZZktKEWFNUC9OUBvVQ>?	NEfUTmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzN\|c6PSd-MUm5N\|M4QTV:L3G+
human PBMC	M2XGXmZ2dmO2aX;uJIF{e2G7		M3v1VlEhcA>?	MV;BcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIGDCUWMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidnSrLVPEN{9ETDJ6IHHueIljd2S7LXnu[JVk\WRiSVytNkBxem:mdXP0bY9vKHS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJIFvfGlvQ1SzM2NFOjhiYX70bYJw\HliY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJIJ6KGmvbYXuc5N2eHC{ZYPzbY9vKGG\|c3H5MEBGSzVyPUOuNUBvVQ>?	M3\GSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOzO\|k2Lz5zOUmzN\|c6PTxxYU6=
Jurkat cells	NYDvN5RbTnWwY4Tpc44h[XO\|YYm=			MVXJcY12dm:\|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTD3ZZMhdWWjc4Xy[YQh[nliaX7obYJqfGmxbjDv[kB1cGViSVytNkBxem:mdXP0bY9vKGmwIFr1dotifCClZXzsd{whUUN3ME2yJI5O	NXr6blRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS2OFM{OzdpPkG0OlQ{OzN5PD;hQi=>
HepG2 cells	MYPGeY5kfGmxbjDhd5NigQ>?			M{TzVGlvcGmkaYTpc44hd2ZibHn2[ZIhe3SjZ3WgVIxie22xZHn1cUBj\XKpaHXpJIlv\mWldHnvckBqdiCKZYDHNkBk\WyuczygTWM2OD1zLk[1JI5O	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6NkGyOEc,OjJ3OE[xNlQ9N2F-
Jurkat	M1X6WWZ2dmO2aX;uJIF{e2G7			NUn4cnZpXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGmwaHnibZRqd25ib3[gTWwuOiC{ZXzlZZNmKHO2aX31cIF1\WRid3n0bEBRVUFxaX;uc416[2mwIHL5JGp2emujdDDj[Yxtey5uIFnDOVAhRSByLkCxNkDPxE1w	Mn;XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzR5M{W0N{c,PzR5M{W0N\|ww[T5?
mouse spleen cells	M3LybGZ2dmO2aX;uJIF{e2G7			NFTaNY1VcGViY3;tdI92dmRid3HzJJRme3SnZDDpckB3cXS{bzDmc5IhcW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9vKGmwIFLESlEhdW:3c3Wgd5Bt\WWwIHPlcIx{KHO2aX31cIF1\WRid3n0bEBkd26lYX7heoFtcW5iQTCoR49vSSlwLDDJR\|UxKD1iMD6wOEDPxE1w	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek84PDd\|NUSzK\|44PDd\|NUSzQE9iRg>?
Sf9	MX7GeY5kfGmxbjDhd5NigQ>?			NYLzU4tpcW6qaXLpeIlwdiCxZjDW[ZJieGGvaXygd5RqdXWuYYTl[EBCXFBiaInkdo9tgXOrczDpckBu\W2kcnHu[ZMh\nKxbTDNSHIyNWW6cILld5NqdmdiU3[5JINmdGy\|LDDLbUA:KDBwMEKg{txONg>?	Mo\XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwPzVzNEK2N{c,PzVzNEK2N\|ww[T5?
LLC-PK1	NX[zNpY{TnWwY4Tpc44h[XO\|YYm=			MYH0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIHnuJG1FWjFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNbTC9JFgvPCEQvF2u	NGrBd3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95NkixNFU6Lz55NkixNFU6RC:jPh?=
HeLa	NYjZUopHTnWwY4Tpc44h[XO\|YYm=			NGna[JpqdmirYnn0bY9vKG:oIFzUR\|QhfXC2YXvlJIlvKG2nbXLyZY5mKH[nc3njcIUh\nKxbTDNVnAyNWW6cILld5NqdmdiSHXMZUBk\WyuczygT4khRSB3IN88UU4>	NYTLbJBXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{m2NVcxPid-N{m2NVcxPjxxYU6=
Caco-2	M4HKc2Z2dmO2aX;uJIF{e2G7			NEjzOpd1emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JEhj[XOjbDD0c{BieGmlYXypJIlvKEOjY3:tNkBk\WyuczygT40hRSB\|Lkig{txONg>?	NUnmTG9[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEi0NlQ2Oid-OEi0NlQ2OjxxYU6=
spleen	MV3GeY5kfGmxbjDhd5NigQ>?			M2[yUGVn\mWldDDv[kBkd22yb4Xu[EBwdiCLTD2yJJBzd2S3Y4Tpc44h\nKxbTDhcIxw[W62aXflck1{fGmvdXzheIVlKG2xdYPlJJNxdGWnbjDj[YxteyxiSVO1NEA:KDBwMEGg{txONg>?	M4rXWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh6OUO4N\|koRjh6OUO4N\|k9N2F-
COS	M4nuXmZ2dmO2aX;uJIF{e2G7			MVzpcohq[mm2aX;uJI9nKFSjdYLvZ4hwdGG2ZTD1dJRic2ViaX6gRXNDXC2neIDy[ZN{cW6pIFPPV{Bk\WyuczygT4khRSB{NDFOwG0v	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86PDV6N{i1K\|46PDV6N{i1QE9iRg>?
LLC-PK1	MljvSpVv[3Srb36gZZN{[Xl?			MljjbY5pcWKrdHnvckBw\iCGb4jvdpVjcWOrbjD0doFve2WyaYTo[Yxq[WxidILhcpNxd3K2IDjiZZNidCC2bzDhdIlk[WxrIDjEbYdwgGmwOjCwMlghfU1rIHnuJG1FWjFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCLQ{WwJF0hOy54NjFOwG0v	M{L0[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl7MUS3PVIoRjl7MUS3PVI9N2F-
CEM/VLB100	MX;GeY5kfGmxbjDhd5NigQ>?			MkfIbY5pcWKrdHnvckBw\iCFYXzj[YlvNUGPIHXm[ox2gCCrbjDDSW0wXkyEMUCwJINmdGy\|LDDLbUA:KDFwODFOwG0v	M3jVcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMUm4NlI4Lz5zMEG5PFIzPzxxYU6=
HEK	MojkSpVv[3Srb36gZZN{[Xl?			MVzpcohq[mm2aX;uJI9nKEKPRzD1dJRic2ViaX6gcYVu[nKjbnWgeoV{cWOuZYOg[pJwdSCPcoCyMYV5eHKnc4PpcochUEWNIHPlcIx{NCCNaTC9JFExKM7:TT6=	MlzJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB2MkG2OVgoRjFyNEKxOlU5RC:jPh?=
HEK	NHzVdmlHfW6ldHnvckBie3OjeR?=			MlribY5pcWKrdHnvckBw\iCETVegeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHXzJIZzd21iTWLQNk1mgHC{ZYPzbY5oKEiHSzDj[YxteyxiS3mgQUAzOSEQvF2u	NU\3eGV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0NlE3PThpPkGwOFIyPjV6PD;hQi=>
HeLa	MUDGeY5kfGmxbjDhd5NigQ>?			MWPpcohq[mm2aX;uJI9nKFSjdYLvZ4hwdGG2ZTD1dJRic2ViaX6gUnREWC2neIDy[ZN{cW6pIFjlUIEh[2WubIOsJGlEPTBiPTCxJO69VS5?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODV4NUi0N{c,OTB3NkW4OFM9N2F-
LLC-PK1	NFLOc2dHfW6ldHnvckBie3OjeR?=			MY\pcohq[mm2aX;uJI9nKEyWQ{SgeZB1[WunIHnuJI1mdWK{YX7lJJZme2mlbHXzJIZzd21iTWLQNk1mgHC{ZYPzbY5oKEyOQzDQT\|Eh[2WubIOsJGtqKD1iND63JO69VS5?	NGrUcHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEW3NFA1QSd-MUC1O\|AxPDl:L3G+
Sf9	NFnZUlhHfW6ldHnvckBie3OjeR?=			NFjoW4dqdmirYnn0bY9vKG:oIGTheZJw[2ixbHH0[UB2eHSja3WgbY4hdWWvYoLhcoUhfmW\|aXPs[ZMh\nKxbTDCd4VxNWW6cILld5NqdmdiU3[5JINmdGy\|LDDLbUA:KDBwMzFOwG0v	MnzUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB4NEi0O\|AoRjFyNkS4OFcxRC:jPh?=
Caco-2	MnfiSpVv[3Srb36gZZN{[Xl?			NYDXXWJjcW6qaXLpeIlwdiCxZjDEbYdwgGmwIITyZY5{\XCrdHjlcIlidCC2cnHud5BwenRiKHLhd4FtKHSxIHHwbYNidCliKFTp[495cW58IEWgeW0qKGmwIFPhZ48uOiClZXzsd{whUUN3MDC9JFEvOyEQvF2u	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODh{MEGzO{c,OTB6MkCxN\|c9N2F-
CEM/VLB100	NXzzPFIzTnWwY4Tpc44h[XO\|YYm=			NGjDRmNqdmirYnn0bY9vKG:oIFPhcINmcW5vQV2g[YZndHW6IDjDZYxk\WmwLVHNPkAxNjJ3IIXNLUBqdiCFRV2vWmxDOTByIHPlcIx{NCCLQ{WwJF0hOy52MTFOwG0v	NYW2XlFlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4PVEyOTRpPkGwPFkyOTF2PD;hQi=>
Sf9	NIewSZNHfW6ldHnvckBie3OjeR?=			MYnpcohq[mm2aX;uJI9nKEGWUHHz[UBqdiCvZX3idoFv\SC4ZYPpZ4xmKG[{b32gRmNTWC2neIDy[ZN{cW6pIGPmPUBk\WyuczygT4khRSByLkWg{txONg>?	NYf3O4F3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG0N\|c{QDBpPkGxOFM4OzhyPD;hQi=>
NIH-3T3-G185	NVPPR3ZrTnWwY4Tpc44h[XO\|YYm=			NEP1VI9qdmirYnn0bY9vKG:oIFTheY5wenWkaXPpckBm\m[udYigbY4hVkmKLUPUN{1IOTh3IHPlcIx{NCCLQ{WwJF0hOS52IN88UU4>	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	NF;6PJhHfW6ldHnvckBie3OjeR?=			NVnYOXVZcW6qaXLpeIlwdiCxZjDDZYxk\WmwLVHNJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUCgQUAyNjdizszNMi=>	Mk\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	NVL2[XlWTnWwY4Tpc44h[XO\|YYm=			M{H4XIlvcGmkaYTpc44hd2ZiUnjv[IFucW6nIEGyN{Bm\m[udYigbY4hVkmKLUPUN{1IOTh3IHPlcIx{NCCLQ{WwJF0hOS55IN88UU4>	M1XDU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{G2OVE1Lz5zMUexOlUyPDxxYU6=
NIH-3T3-G185	M13xV2Z2dmO2aX;uJIF{e2G7			NWPUOoo4cW6qaXLpeIlwdiCxZjDMSHMuPzVzIHXm[ox2gCCrbjDOTWguO1R\|LVexPFUh[2WubIOsJGlEPTBiPTCxMlgh\|ryPLh?=	Mlj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	M4jTbmZ2dmO2aX;uJIF{e2G7			NHLQZoxqdmirYnn0bY9vKG:oIGTleJJidWW2aInsdo9{[W2rbnWg[YZndHW6IHnuJG5KUC1\|VEOtS\|E5PSClZXzsd{whUUN3MDC9JFIvOSEQvF2u	NUOwNGE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NVY2OTRpPkGxO\|E3PTF2PD;hQi=>
NIH-3T3-G185	MmnySpVv[3Srb36gZZN{[Xl?			MX;pcohq[mm2aX;uJI9nKE[udX:tN{1CVSCnZn\seZghcW5iTlnIMVNVOy2JMUi1JINmdGy\|LDDJR\|UxKD1iMj60JO69VS5?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
NIH-3T3-G185	M{CyV2Z2dmO2aX;uJIF{e2G7			MX7pcohq[mm2aX;uJI9nKEqFLUGg[YZndHW6IHnuJG5KUC1\|VEOtS\|E5PSClZXzsd{whUUN3MDC9JFQvOyEQvF2u	NWPyXmFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NVY2OTRpPkGxO\|E3PTF2PD;hQi=>
HCT15	M2[xOGZ2dmO2aX;uJIF{e2G7	M1zzOFAvPCC2bzC2MlMhfWdxbVy=		M1PLOm1w\HWuYYTpc44hd2ZiUD3ndEBqdiCqdX3hckBJS1RzNTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBFUU6LQj3pcoR2[2WmIHP5eI91d3irY3n0fUBifCByLkSgeI8hPi5\|IIXnM41NNg>?	MlS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5NUS2NFIoRjFzN{W0OlAzRC:jPh?=
UO31	MYnGeY5kfGmxbjDhd5NigQ>?	MXOwMlQhfG9iNj6zJJVoN22O		MoKyUY9lfWyjdHnvckBw\iCSLXfwJIlvKGi3bXHuJHVQOzFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gSGlPUUJvaX7keYNm\CCleYTveI95cWOrdImgZZQhOC52IITvJFYvOyC3Zz;tUE4>	M2LPZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{W0OlAzLz5zMUe1OFYxOjxxYU6=
CAKI1	MlrSSpVv[3Srb36gZZN{[Xl?	MkLzNE41KHSxIE[uN{B2\y:vTB?=		MWLNc4R2dGG2aX;uJI9nKFBvZ4CgbY4hcHWvYX6gR2FMUTFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gSGlPUUJvaX7keYNm\CCleYTveI95cWOrdImgZZQhOC52IITvJFYvOyC3Zz;tUE4>	M4Tvc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{W0OlAzLz5zMUe1OFYxOjxxYU6=
MDA-435/LCC6 and MDA-435/LCC6-MDRI	NYi5fWFlTnWwY4Tpc44h[XO\|YYm=			NU\DeFlSS2:wY3XueJJifGmxbjD0bIF1KHKnZIXj[ZMhfGinIHTp[oZmemWwY3WgbY4hemW4ZYLzZYwhd2ZiRF;YJIFk[3WvdXzheIlwdiCkZYT3[YVvKE2GQT20N\|UwVEOFNjDhcoQhVUSDLUSzOU9NS0N4LV3EVmkh[2WubIOgZpkhPTBnLjygSWM2OCB;IECuOUDPxE1w	MlLEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6	NYLIUIVjTnWwY4Tpc44h[XO\|YYm=			MUDF[oZm[3Rib36gdoV3\XK\|YXygc4YhYzOKXT2gWmJNKGGlY4XteYxifGmxbjDpckBOTEFvNEO1M2xESzZiKGDndE1v\WejdHn2[Ukh[2WubIOuMEBGSzVyIE2gNE43KM7:TT6=	M1HlNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA-435/LCC6 and MDA-435/LCC6-MDRI	NYPiWldoTnWwY4Tpc44h[XO\|YYm=			NIDQbnZEd26lZX70doF1cW:wIIToZZQhemWmdXPld{BlcW[oZYLlcoNmKGmwIILleoVze2GuIH;mJHs{UF2YQlygZYNkfW23bHH0bY9vKGKndIfl[Y4hVUSDLUSzOU9NS0N4IHHu[EBOTEFvNEO1M2xESzZvTVTSTUBk\WyuczDifUA2OCVuIFXDOVAhRSByLk[g{txONg>?	M1[2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA435/LCC6 MDR1	M1nvXmZ2dmO2aX;uJIF{e2G7			MmnvdoV3\XK\|YXygc4YhXmmwYnzhd5RqdmViYXPjeY12dGG2aX;uJEhXcW6kbHHzeIlv\TpiMD6wNFUhfU1rIHnuJG1FSTR\|NT;MR2M3KE2GUkGgZ4VtdHNuIFXDOVAhRSByLk[g{txONg>?	M2rtN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA-435/LCC6 (Pgp-negative)	NFzXXopHfW6ldHnvckBie3OjeR?=			M1XIR2Vn\mWldDDvckBz\X[ncoPhcEBw\iCGT2igZYNkfW23bHH0bY9vKGmwIF3ERU01OzVxTFPDOkApWGeyLX7l[4F1cX[nKTDj[Yxtey5uIFXDOVAhRSByLkeg{txONg>?	MmPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6	MULHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>?			M4jRT2dzd3e2aDDpcohq[mm2b4L5JIFkfGm4aYT5JI9vKE2GQT20N\|UwVEOFNjCoVIdxNXCxc3n0bZZmMSCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIx{NixiSVO1NEA:KDFizszNMi=>	M1PHZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA-435/LCC6-MDRI	MUnHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>?			NEHkZ3dIem:5dHigbY5pcWKrdH;yfUBi[3Srdnn0fUBwdiCPRFGtOFM2N0yFQ{[tUWRTUSBqUHfwMY5m\2G2aY\lLUBpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHzzMkwhUUN3MDC9JFEvOSEQvF2u	MmHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
BTI-TN5B1-4	NYD6cFBQTnWwY4Tpc44h[XO\|YYm=			NHOyNoZCXFBiaInkdo9tgXOrczDpckBu\W2kcnHu[UBnemGldHnvckBnem:vIFjp[4ghTmm4ZTCoRnRKNVSQNVKxMVQqKGOnbHzzMEBMdSB;IECuNVch\|ryPLh?=	NH\2UWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OVY5PCd-MUG3PFU3QDR:L3G+
Jurkat	MVjGeY5kfGmxbjDhd5NigQ>?			NHnHWJZKdmirYnn0bY9vKG:oIIDyc4R2[3Srb36gc4YhUUxvMjDz[YNz\XSrb36gbY4hUnW{a3H0JINmdGy\|LDDJR\|UxKD1iMD6wNFch\|ryPLh?=	MknNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6OEG5PFQoRjFzOEixPVg1RC:jPh?=
Jurkat	NVG1NHhHTnWwY4Tpc44h[XO\|YYm=			M2ToZWlvcGmkaYTpc44hd2ZicILv[JVkfGmxbjDv[kBj\XSjLXfhcIFkfG:\|aXThd4UhUUxvMjDSS2EhcW5iSoXyb4F1KGOnbHzzMEBKSzVyIE2gNE4xODhizszNMi=>	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTh6MUm4OEc,OTF6OEG5PFQ9N2F-
LLC-PK1	NGDqV2NHfW6ldHnvckBie3OjeR?=			NXvMXZp3cW6lcnXhd4UhcW5iVnnuZoxie3SrbnWgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gbY4hVUSUMT3lfJBz\XO\|aX7nJGxNSy2SS{GgZ4VtdHNuIFvpJF0hOS5\|IN88UU4>	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTl4MUGxN{c,OTF7NkGxNVM9N2F-
LLC-PK1	NF;2NXdHfW6ldHnvckBie3OjeR?=			MnHTbY5kemWjc3WgbY4hS2GuY3Xpck1CVSCrboTyZYNmdGy3bHHyJIFk[3WvdXzheIlwdiCrbjDNSHIyNWW6cILld5NqdmdiTFzDMXBMOSClZXzsd{whU2liPTC0MlY3KM7:TT6=	NYTYNmxFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5OlEyOTNpPkGxPVYyOTF\|PD;hQi=>
MDCK	MV3GeY5kfGmxbjDhd5NigQ>?			M{K2PIlvcGmkaYTpc44hd2ZiRHnnc5hqdiC2cnHud4VxcXSqZXzpZYwhfHKjboPwc5J1KCikYYPhcEB1dyCjcHnjZYwqKGmwIF3EVlEu\XiycnXzd4lv\yCPRFPLJINmdGy\|LDDLbUA:KDJwMUig{txONg>?	NHf2NXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGzOFk1PSd-MUKxN\|Q6PDV:L3G+
MDCK	M3XETmZ2dmO2aX;uJIF{e2G7			NVLFfo8zcW6qaXLpeIlwdiCxZjDWbY5jdGG\|dHnu[UB1emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JEhj[XOjbDD0c{BieGmlYXypJIlvKE2UUEKt[ZhxemW\|c3nu[{BOTEONIHPlcIx{NCCNaTC9JFgvOTFizszNMi=>	NYrK[HR5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxN\|Q6PDZpPkGyNVM1QTR4PD;hQi=>
LLC-PK1	NWnuV4pKTnWwY4Tpc44h[XO\|YYm=			MYTpcoNz\WG\|ZTDpckBE[WylZXnuMWFOKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKGmwIH3kdlFjNWW6cILld5NqdmdiTFzDMXBMOSClZXzsd{whU2liPTCzMlUh\|ryPLh?=	NXe1[HZDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN\|UzPjdpPkGyNlM2OjZ5PD;hQi=>
LLC-PK1	NXXlRoQxTnWwY4Tpc44h[XO\|YYm=			M3rwbolv[3KnYYPlJIlvKEOjbHPlbY4uSU1iaX70doFk\WyudXzhdkBi[2O3bYXsZZRqd25iaX6gcYRzOWFvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNaTC9JFMvPjVizszNMi=>	NFHJRo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkKzOVI3Pyd-MUKyN\|UzPjd:L3G+
HL-60	MkTRSpVv[3Srb36gZZN{[Xl?			Mn\rTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIH\vdo16dHCncITp[IUhemWlZYD0c5IhME[SUjmgc4YhcHWvYX6gcIV2c2WvaXGgTGwuPjBiY3XscJMtKEmFMkCgQUA{NjZizszNMi=>	M1e2VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN\|g5Lz5zMkO2NVM5QDxxYU6=
High Five cells	NI\ZUZdHfW6ldHnvckBie3OjeR?=			NWLzToVVcW6qaXLpeIlwdiCxZjDUZZVzd2OqZX7v[IVwgHmlaH;sZZRmKHWydHHr[UhEe0FiM{D1UUwhOzBiJTDv[kBkd262cn;sLUBqdiCvZX3idoFv\SC4ZYPpZ4xmeyCycnXwZZJm\CCocn;tJGhq\2hiRnn2[UBk\WyuczDpcoZm[3SnZDD3bZRpKHSqZTDBRmNDOTFiYnHjeYxwfmm{dYOsJGtqKD1iOT61JO69VS5?	MknsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MESyN\|koRjF{NEC0NlM6RC:jPh?=
MDCKII	MWHGeY5kfGmxbjDhd5NigQ>?			M3zxNIlvcGmkaYTpc44hd2ZiQ3XybZZie3SjdHnuJJVxfGGtZTDpckBQSVSSMj3lfJBz\XO\|aX7nJG1FS0uLSTDj[YxteyxiS3mgQUAxNjJ\|ODFOwG0v	M4LPRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NUO4PFE{Lz5zMkWzPFgyOzxxYU6=
Caco-2	M1fhXmZ2dmO2aX;uJIF{e2G7			NIixdW51emGwc3XwbZRp\WyrYXygeJJidnOyb4L0JI9nKGSrZ3;4bY4hMGKjc3HsJJRwKGGyaXPhcEkhcW5iQ3Hjc{0zKGOnbHzzMEBMcSB;IECuN\|Uh\|ryPLh?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZ\|NkG1N{c,OTJ4M{[xOVM9N2F-
SK-E2	MnXwSpVv[3Srb36gZZN{[Xl?			NHjXRmVqdmO{ZXHz[UBqdiCkb3TpdJkhcW62cnHj[YxtfWyjcjDhZ4N2dXWuYYTpc44hMEKxZHnwfVohOC5{IIXNLUBqdiCVSz3FNkBk\WyuczCo[ZhxemW\|c3nu[{BDW0WSKTygTWM2OCB;IEeuOUDPxE1w	NV3oS2p{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3N\|k4PTlpPkGyO\|M6PzV7PD;hQi=>
SK-E2	MX3GeY5kfGmxbjDhd5NigQ>?			NFH3OppqdmO{ZXHz[UBqdiCmaXj5[JJw\my3b4Lld4NmcW5iaX70doFk\WyudXzhdkBi[2O3bYXsZZRqd25iKHTpbJllem:obIXvdoV{[2WrbkqgNUB2VSliaX6gV2suTTJiY3XscJMhMGW6cILld5NqdmdiQmPFVEktKEmFNUCgQUA4NjhizszNMi=>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjd\|OUe1PUc,OTJ5M{m3OVk9N2F-
HEK293	NHfrW5BHfW6ldHnvckBie3OjeR?=			NXnMU5lJcW6qaXLpeIlwdiCxZjDQbIFtdG:rZHnuJJVxfGGtZTCoVIhidGyxaXTpclohOSC3TTmgbY4hV0GWUD3DMYV5eHKnc4PpcochUEWNMkmzJINmdGy\|LDDLbUA:KDBwMjFOwG0v	NYDNe\|F6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1N\|A6ODdpPkG0OVMxQTB5PD;hQi=>
T-cells	M2TGUmZ2dmO2aX;uJIF{e2G7			NVu5TI5IUW6qaXLpeIlwdiCxZjDhcIxw\2WwaXOgZ4VtdHNvc4TpcZVt[XSnZDDwdo9tcW[ncnH0bY9vKGmwIH3veZNmKFRiY3XscJMh[nlibXn4[YQhdHmvcHjvZ5l1\SC{ZXHjeIlwdiCvZYToc4QtKEmFNUCgQUAxNjB\|MjFOwG0v	MofNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3OUOxPFIoRjF2NUmzNVgzRC:jPh?=
T-cells	M3TLWWZ2dmO2aX;uJIF{e2G7			NIK5WVVKdmirYnn0bY9vKG:oIHHscI9o\W6rYzDj[Yxtey2\|dHnteYxifGWmIIDyc4xq\mW{YYTpc44hcW5iaIXtZY4hXCClZXzsd{BjgSCvaYjl[EBtgW2yaH;jfZRmKHKnYXP0bY9vKG2ndHjv[EwhUUN3MDC9JFAvODd2IN88UU4>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV7M{G4Nkc,OTR3OUOxPFI9N2F-
PMBC	MW\GeY5kfGmxbjDhd5NigQ>?		MXq3NkBpenN?	MorDSYZn\WO2IH;uJJBzd2yrZnXyZZRqd25ib3[gbJVu[W5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyBqUF3CR{kh[XRiN{KgbJIhfXOrbnegUXRVKGG\|c3H5MEBKSzVyIE2gPE46PyEQvF2u	Mlj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV{MEOxOlMoRjF3MkCzNVY{RC:jPh?=
mouse spleen cells	MWXDfZRwfG:6aXPpeJkh[XO\|YYm=			Mmm4R5l1d3SxeHnjbZR6KGGpYXnud5QhSkGOQj;jJI1wfXOnIIPwcIVmdiClZXzsd{whS0N3MDC9JFEvOzVizszNMi=>	M164XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEixO\|YyLz5zN{C4NVc3OTxxYU6=
MDCK2	NITBRlRHfW6ldHnvckBie3OjeR?=			NXjufW0{UW6qaXLpeIlwdiCxZjDoeY1idiCPRGKxMYRmeGWwZHXueEBi[2O3bYXsZZRqd25ib3[gZ4Ft[2Wrbj3BUUBmgHC{ZYPz[YQhcW5iTVTDT\|Ih[2WubIOsJGlEPTBiPTC5MlM1KM7:TT6=	M4XqOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[0N\|I4Lz5zN{[2OFMzPzxxYU6=
HEK293	NI\EN4hHfW6ldHnvckBie3OjeR?=			MYHJcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPMTDMOlVTN1SPNjDMN\|M6Sy:WTUegSlczQCCvdYThcpQh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdiNUClJIlvcGmkaYTpc44hd2ZiY4Lvd5MhdGmwa3nu[{BjgSCrbX31co9jdG:2LDDBZ5Rqfmm2eTC9JFAvPSEQvF2u	NEDFZXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i0PFU3Oyd-MUe4OFg2PjN:L3G+
HEK293	M171TWZ2dmO2aX;uJIF{e2G7			M{DuVWlvcGmkaYTpc44hd2ZiY4Lvd5MudGmwa3nu[{Bw\iCqdX3hckBR\3BiVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDBwNjFOwG0v	NICwW\|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i0PFU3Oyd-MUe4OFg2PjN:L3G+
HEK293	M37jfWZ2dmO2aX;uJIF{e2G7			M1LEfWlvcGmkaYTpc44hd2ZiY4Lvd5MudGmwa3nu[{Bw\iCqdX3hckBR\3BiVF2zWFE6QVJxVF22JGw{OzmFL2TNO{BHPzJ6QzDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegOVAmKGmwaHnibZRqd25ib3[gZ5Jwe3NibHnub4lv\yCkeTDpcY12dm:kbH;0MEBC[3Srdnn0fUA:KDFwMTFOwG0v	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293	MkTISpVv[3Srb36gZZN{[Xl?			NUjoeoVHUW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVYhTjN2M2KvWG03KEx\|M{nDM3ROPyCIN{K4R{BufXSjboSg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKGOjdYPpcochPTBnIHnubIljcXSrb36gc4Yh[3Kxc4OgcIlvc2mwZzDifUBqdW23bn;icI91NCCDY4Tpeol1gSB;IEGxJO69VS5?	M3PDeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OES4OVY{Lz5zN{i0PFU3OzxxYU6=
HEK293	NH\CUGtHfW6ldHnvckBie3OjeR?=			M3PNOWlvcGmkaYTpc44hd2ZiY4Lvd5MudGmwa3nu[{Bw\iCqdX3hckBR\3BiVF21JGk{OD[UL2TNOkBNOzN7Qz;UUVchTjd{OFOgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW\|aX7nJFUxLSCrbnjpZol1cW:wIH;mJINzd3O\|IHzpcotqdmdiYomgbY1ufW6xYnzveEwhSWO2aY\peJkhRSBzNTFOwG0v	NG\xWpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i0PFU3Oyd-MUe4OFg2PjN:L3G+
MDCK2	MV;GeY5kfGmxbjDhd5NigQ>?	NWfnSWRMOTBidV2=	M37lXlQxKG2rboO=	MnHyTY5pcWKrdHnvckBw\iCqdX3hckB4cWymIIT5dIUhWGeyIHX4dJJme3OnZDDpckBOTEONMjDj[YxteyCjc4Pld5Nm\CCjczDjZYxk\WmwLVHNJIFk[3WvdXzheIlwdiCjdDCxNEB2VSCjZoTldkA1OCCvaX7zMi=>	Mk\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NkSxO\|AoRjF5OU[0NVcxRC:jPh?=
A2780	MmHjSpVv[3Srb36gZZN{[Xl?		NVPUUWExOzBibXnudy=>	MmDSTY5pcWKrdHnvckBw\iCqdX3hckBR\3BiaX6gRVI4QDBiY3XscJMh[W[2ZYKgN\|AhdWmwczDifUBJd2WlaIP0JFM{OzR{IHHzd4F6NCCLQ{WwJF0hOS52MUK1OEDPxE1w	NEP1THE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4N\|A{PCd-MUiwPFMxOzR:L3G+
A2780	MWPGeY5kfGmxbjDhd5NigQ>?		M{PobVMxKG2rboO=	M2DUPGlvcGmkaYTpc44hd2ZiaIXtZY4hWGeyIHnuJGEzPzhyIHPlcIx{KGGodHXyJFMxKG2rboOgZpkh[2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFEvPDF{NUSg{txONg>?	MmW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyOEOwN\|QoRjF6MEizNFM1RC:jPh?=
Caco-2	MV7GeY5kfGmxbjDhd5NigQ>?			M1LNOWlvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4udWWmaXH0[YQhYzOKXY\pcoJt[XO2aX7lJJRz[W6\|cH;yeEBqdiCqdX3hckBE[WOxLUKgZ4VtdHNuIFXDOVAhRSB2NT61JO69VS5?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV{NEW5Nkc,OTh3MkS1PVI9N2F-
A2780	NUjaT2VYTnWwY4Tpc44h[XO\|YYm=			MonZTY5pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJIFlemmjbYnjbY4uemW\|aYP0ZY51KEF{N{iwJINmdGy\|IHL5JGhw\WOqc4SgN\|M{PDJiYYPzZZktKEmFNUCgQUAyNjRzMkW0JO69VS5?	Ml\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4N{i0PVUoRjF6Nke4OFk2RC:jPh?=
Huh-9-13	NX;iRpJLSW62aY\pdoFtKGG\|c3H5		M33acVMh\GG7cx?=	M2fmXmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGJKVE5iMkC2NU1z\XOrc4ThcpQhUEOYIHflco91gXCnIEHiJIlv\mWldHXkJIlvKGi3bXHuJGh2cC17LUGzJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA{KGSjeYOgZpkheXWjboTpeIF1cX[nIGLUMXBEWixiRVO1NEA:KDBwMEC0JO69VS5?	MniyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	NVL6S2c2SW62aY\pdoFtKGG\|c3H5		NYLnNok3OyCmYYnz	M3\QT2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JINwdXCxdX7kMZJme2m\|dHHueEBJS1ZiZ3Xuc5R6eGViMXKgbY5n\WO2ZXSgbY4hcHWvYX6gTJVpNTlvMUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFMh\GG7czDifUByfWGwdHn0ZZRqfmViUmStVGNTNCCHQ{WwJF0hOC5\|NTFOwG0v	MkjjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	M1rxc2FvfGm4aYLhcEBie3OjeR?=		Mnv6N{Bl[Xm\|	MmrxRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgNkcuSy2vZYTofYwuOidvZnz1c5JwNWO7dHnkbY5mNXKnc3nzeIFvfCCKQ2[g[4Vvd3S7cHWgNYIhcW6oZXP0[YQhcW5iaIXtZY4hUHWqLUmtNVMh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDNiZHH5d{BjgSCzdXHueIl1[XSrdnWgVnQuWEOULDDFR\|UxKD1iMD60JO69VS5?	M2LmTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUC0NFE{Lz5zOUGwOFAyOzxxYU6=
Huh-9-13	M4njWGFvfGm4aYLhcEBie3OjeR?=		NXXyU3dEOyCmYYnz	NIPuNpBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBXYCB7NUCtdoV{cXO2YX70JGhEXiCpZX7veJlx\SBzYjDpcoZm[3SnZDDpckBpfW2jbjDIeYguQS1zMzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgN{Bl[Xm\|IHL5JJF2[W62aYTheIl3\SCUVD3QR3ItKEWFNUCgQUAxNjR7IN88UU4>	M3Ty[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUC0NFE{Lz5zOUGwOFAyOzxxYU6=
Huh-9-13	NFq4eYdCdnSrdnnyZYwh[XO\|YYm=		NXP6[oxFOyCmYYnz	M165dmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHI1PzlvcnXzbZN1[W62IFjDWkBo\W6xdInw[UAy[iCrbn\lZ5Rm\CCrbjDoeY1idiCKdXitPU0yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KsJGVEPTBiPTCxMlk5KM7:TT6=	MkW4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzMESwNVMoRjF7MUC0NFE{RC:jPh?=
Huh-9-13	M1vPOmFvfGm4aYLhcEBie3OjeR?=		MYGzJIRigXN?	MYjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDjfYNtd3Oyb4LpcoUuemW\|aYP0ZY51KEiFVjDn[Y5wfHmyZTCxZkBqdm[nY4Tl[EBqdiCqdX3hckBJfWhvOT2xN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyZYwhemWybHnjZZRqd25iYX\0[ZIhOyCmYYnzJIJ6KHG3YX70bZRifGm4ZTDSWE1RS1JuIFXDOVAhRSB\|Lki1JO69VS5?	NHzqfpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGwOFAyOyd-MUmxNFQxOTN:L3G+
A2780/ADR	MXvGeY5kfGmxbjDhd5NigQ>?			NHXweGFKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNW2nZHnheIVlKG23bITp[JJ2\yC{ZYPpd5RidmOnIHnuJIFlemmjbYnjbY4uemW\|aYP0ZY51KGi3bXHuJGEzPzhyL1HEVkBk\WyuczDifUBk[WylZXnuJGFOKGG\|c3H5MEBKSzVyIE2gNU41KM7:TT6=	NIe0WJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK1NFg{PCd-MUmyOVA5OzR:L3G+
C2C12	NVTKZVZtTnWwY4Tpc44h[XO\|YYm=	NELlNowxNjFidXevcYw>	MkDHN{Bl[Xm\|	MXnJcohq[mm2aX;uJI9nKGineH;z[U03NXCqb4PwbIF1\SCmZXj5[JJw\2WwYYPlMYlv\HWlZXSgZ4VtdCCmaX\m[ZJmdnSrYYTpc44hcW5ibX;1d4UhSzKFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgcZlwe2mwIHjlZZZ6KGOqYXnuJIZtfW:{ZYPj[Y5k\SCjdDCwMlEhfWdxbXyg[o9zKDNiZHH5d{4>	Mnu3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByOEG4OVUoRjJyMEixPFU2RC:jPh?=
C2C12	NWq0SlVmTnWwY4Tpc44h[XO\|YYm=			MkjiTY5pcWKrdHnvckBw\iC\|ZYL1cUB4cXSqZILhe4FtNWmwZIXj[YQh[2WubDDkbYZn\XKnboTpZZRqd25iaX6gcY92e2ViQ{LDNVIh[2WubIOgZZN{\XO\|ZXSgZZMhdXmxc3nuJIhm[X[7IHPoZYlvKGW6cILld5Nqd25w	NGTNbJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC4NVg2PSd-MkCwPFE5PTV:L3G+
HuH7	MYnBcpRqfmm{YXygZZN{[Xl?		NHW4cXQ1QCCqcoO=	M2P6UGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEXiC\|dXL0fZBmKDGkIFPvclEhcW6oZXP0[YQhcW5iaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygVm5CKGyndnXsJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO\|YYmsJGVEPTBiPTCwMlIyKM7:TT6=	M4DNeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUe2PFk1Lz5{MEG3Olg6PDxxYU6=
HuH7	NFnMUIZCdnSrdnnyZYwh[XO\|YYm=		MmjJOFghcHK\|	MVXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDjfYNtd3Oyb4LpcoUhSS2{ZYPpd5RidnRiSFPWJJN2[nS7cHWgNYIhS2:wMTDpcoZm[3SnZDDpckBpfW2jbjDIeWg4KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJidCCUTlGgcIV3\WxiYX\0[ZIhPDhiaILzJIJ6KE2WUzDhd5NigSxiRVO1NEA:KDNwM{mg{txONg>?	NHnMe2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG3Olg6PCd-MkCxO\|Y5QTR:L3G+
T-cells	M1XRS2ludXWwb4P1dJBz\XO\|aY\lJIF{e2G7		NI\vNoc4OiCqcoO=	MoPHTY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hSkGOQj;jJI1wfXOnIGSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjboTpMWNFOy:jboTpMWNFOjhvc4TpcZVt[XSnZDDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFYh\|ryPLh?=	NVy5UHA4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN\|EyODRpPkKxNVMyOTB2PD;hQi=>
T-cells	MnnyR5l1d3SxeHnjbZR6KGG\|c3H5		M2\KWVQ5KGi{cx?=	M2W3e2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KEKDTFKvZ{Bud3W\|ZTDUJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFPDOVAhRSBzNT64NkDPxE1w	M1fMRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUOxNVA1Lz5{MUGzNVExPDxxYU6=
HCT15	M2\aeGZ2dmO2aX;uJIF{e2G7	MX[xNEB2VQ>?		NHW3Sm9KdmirYnn0bY9vKG:oIF3EVlEhcW5iaIXtZY4hUEOWMUWgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4Ft[2Wrbj3BUUBm\m[udYigZZQhOTBidV2gZpkh\my3b4Lld4NmdmOnIHHzd4F6Ng>?	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2OES2NUc,OjF\|NEi0OlE9N2F-
T-cells	M4Hwe2ludXWwb4P1dJBz\XO\|aY\lJIF{e2G7		NFS5eoM6PiCqcoO=	MX7JcY12dm:\|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBDSUyEL3OvR\|U4SkxxNjDtc5V{\SCWIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[Wyub3HueIlo\W5vaX7keYNm\CClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhQTZiaILzJIJ6KG2nYYP1dolv\yCdM1jdeIh6dWmmaX7lJJVxfGGtZTDifUBucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJIF{e2G7LDDJR\|UxKD1iMD6wNVQh\|ryPLh?=	NGTONoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1OlUzPCd-MkG0OVY2OjR:L3G+
mouse spleen cells	MX3GeY5kfGmxbjDhd5NigQ>?			NVvURYFZUW6qaXLpeIlwdiCxZjDz[ZJqdmVicHHscYl1d3mudILhcpNn\XKjc3WgbY4hdW:3c3Wgd5Bt\WWwIHPlcIx{Ng>?	NFrQXJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1OlUzPCd-MkG0OVY2OjR:L3G+
HCT116	M4S1ZWZ2dmO2aX;uJIF{e2G7	NWTKOItzOTBidV2=	NInWO\|k{OCCvaX7z	MoXzUY9lfWyjdHnvckBw\iCEQ2LQJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDv[kBucXSxeHHueJJwdmViYYSgNVAhfU1iYX\0[ZIhOzBibXnud{BjgSCobH;3MYN6fG:vZYTyfU4>	NWW4TlMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0PFg3QDZpPkKxOFg5Pjh4PD;hQi=>
Sf21	M3H6bGZ2dmO2aX;uJIF{e2G7			NULDSVNjUW6qaXLpeIlwdiCxZjDoeY1idiCEU1XQJIV5eHKnc4Pl[EBqdiCybHHzcYEhdWWvYoLhcoUhfmW\|aXPs[ZMhd2ZiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHUVE1l\XCnbnTlcpQhYzOKXYTheZJw[2ixbHH0[UB2eHSja3WsJGlEPTBiPTCwMlUh\|ryPLh?=	NGi3WXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm2OVYzOyd-MkG5OlU3OjN:L3G+
Sf21	NFu4XHlHfW6ldHnvckBie3OjeR?=			NGnQeGhKdmirYnn0bY9vKG:oIGPwdoFofWVvRHH3cIV6KHKjdDDCd4VxKGW6cILld5Nm\CCrbjDwcIF{dWFibXXtZpJidmVidnXzbYNt\XNib3[gV4YzOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGFVWC2mZYDlcoRmdnRiW{PIYZRifXKxY3jvcIF1\SC3cIThb4UtKEmFNUCgQUAxNjZizszNMi=>	Mnu2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
T-cells	M3uwVGZ2dmO2aX;uJIF{e2G7			M{jQbWlvcGmkaYTpc44hd2ZiQ1S0JIV5eHKnc4Ppc44hcW5iaIXtZY4hS0R2KzDUJINmdGy\|LDDJR\|UxKD1iOD65O{DPxE1w	M1XlWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUCxNVM2Lz5{MkGwNVE{PTxxYU6=
L929	Mlj4R5l1d3SxeHnjbZR6KGG\|c3H5		NXPRfoNLPzJiaILz	NFXjboJEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNQTJ7IHPlcIx{KGGodHXyJFczKGi{czygTWM2OCB;IEOzMlkh\|ryPLh?=	MoPMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|MkC0NFIoRjJ{M{KwOFAzRC:jPh?=
CCRF-CEM/VCR1000	NYHaZVFGTnWwY4Tpc44h[XO\|YYm=		MmHJNlQxKHOnY4O=	MXHJcohq[mm2aX;uJI9nKFBvZ3z5Z49xem:2ZXnuMY1m\GmjdHXkJIRifW6xcoXibYNqdiCnZn\seZgh\nKxbTDoeY1idiCFQ2LGMWNGVS:YQ2KxNFAxKGOnbHzzJIFnfGW{IEK0NEB{\WO\|IHL5JGZCS1NiZnzve{BkgXSxbXX0dolkKGGwYXz5d4l{NCCLQ{WwJF0hOC5zMEKzN{DPxE1w	NUfic4F1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OVI1OTJpPkKyOFUzPDF{PD;hQi=>
mouse lymph node cells	MkjUTY1ufW6xc4XwdJJme3OrdnWgZZN{[Xl?		MmjnO\|IhcHK\|	MUPJcY12dm:\|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBidnSrLVPEN{9idnSrLVPEPEBkdy2\|dHnteYxifGWmIFLhcIIw[yCvb4Xz[UBtgW2yaDDuc4RmKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDFUGlUSSxiSVO1NEA:KDBwMEeg{txONg>?	NILrVIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS5OFYyPid-MkK0PVQ3OTZ:L3G+
mouse lymph node cells	NX30ZoZbS3m2b4TvfIlkcXS7IHHzd4F6		NHjXeHY1QCCqcoO=	MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBD[WykL3OgcY92e2VibIntdIghdm:mZTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCFQ{WwJF0hOTBwMTFOwG0v	NFn4PJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS5OFYyPid-MkK0PVQ3OTZ:L3G+
HEK293	MYHGeY5kfGmxbjDhd5NigQ>?		Mo\VOUBucW6\|	NWTtXnhQUW6qaXLpeIlwdiCxZjDoeY1idiCuaY\ldkBQSVSSMVKzJIV5eHKnc4Pl[EBqdiCKRVuyPVMhTmyyLVnuJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCdM1jdSVE4NWKndHHHJJVxfGGtZTDpcoN2[mG2ZXSg[o9zKDVibXnud{BjgSC\|Y3nueIltdGG2aX;uJINwfW62aX7nMEBMcSB;IEGuNkDPxE1w	M13xU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	MnrzSpVv[3Srb36gZZN{[Xl?			MnPlTY5pcWKrdHnvckBw\iCqdX3hckBtcX[ncjDPRXRROUJzIHX4dJJme3OnZDDpckBJTUt{OUOgSoxxNUmwIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBGOTdvYnX0ZWchfXC2YXvlJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqKD1iMT6zJO69VS5?	NXzqd45NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFExPjhpPkKyOVQyODZ6PD;hQi=>
HEK293	MnviSpVv[3Srb36gZZN{[Xl?		M3HZOlUhdWmwcx?=	NGDKXI5KdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFBzQkOg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FNVcu[mW2YVegeZB1[WunIHnuZ5Vj[XSnZDDmc5IhPSCvaX7zJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGlEPTBiPTCxMlMh\|ryPLh?=	M2HZUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	M3nPW2Z2dmO2aX;uJIF{e2G7			MkHDTY5pcWKrdHnvckBw\iCqdX3hckBtcX[ncjDPRXRROUJzIHX4dJJme3OnZDDpckBJTUt{OUOgSoxxNUmwIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBGOTdvYnX0ZWchfXC2YXvlJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGlEPTBiPTCxMlQh\|ryPLh?=	MnH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NEGwOlgoRjJ{NUSxNFY5RC:jPh?=
HEK293	NIXPV3VHfW6ldHnvckBie3OjeR?=		MXK1JI1qdnN?	NHe5SVBKdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFB{QkGg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FN3MhfXC2YXvlJIlv[3WkYYTl[EBnd3JiNTDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurIE2gN\|Yh\|ryPLh?=	M2jPflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
HEK293	NXnY[XdVTnWwY4Tpc44h[XO\|YYm=		MWe1JI1qdnN?	NHzY[WlKdmirYnn0bY9vKG:oIHj1cYFvKGyrdnXyJG9CXFB{QkGg[ZhxemW\|c3XkJIlvKEiHS{K5N{BHdHBvSX6gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFt\|SG3FN3MhfXC2YXvlJIlv[3WkYYTl[EBnd3JiNTDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUA{PyEQvF2u	M3\MUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUSxNFY5Lz5{MkW0NVA3QDxxYU6=
T-cells	NYriUoxUUW2vdX7vd5VxeHKnc4PpeoUh[XO\|YYm=		NF3B[YYzPCCqcoO=	MXjJcY12dm:\|dYDwdoV{e2m4ZTDhZ5Rqfmm2eTDpckBDSUyEL3OgcY92e2ViVDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEOxblGtbY5lfWOnZDDUJINmdGxicILvcIln\XKjdHnvckBqdmO3YnH0[YQhOjRiaILzJJBwe3RiQ3;uRU1qdmS3Y4Tpc44h\m:{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCyMlEzKM7:TT6=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd{N{O2PUc,OjJ5MkezOlk9N2F-
2008	MYPGeY5kfGmxbjDhd5NigQ>?	NGWwSokyOCC3TR?=	NWT4OlMxOzBibXnudy=>	NH\SNFVKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEGg[ZhxemW\|c3XkJIlvKDJyMEigZ4VtdHNiYYSgNVAhfU1icILlbY5kfWKjdHXkJIZweiB\|MDDtbY5{KHC{aX;yJJRwKGOjbHPlbY4hSU1iYXTkbZRqd25ibXXhd5Vz\WRiZY\ldpkhPjBic3Xjd{B2eCC2bzC5NEBucW6\|IHL5JINidGOnaX6gRW0h[WOldX31cIF1cW:wIHHzd4F6Ng>?	Mn[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUe4PFgoRjJ\|MEG3PFg5RC:jPh?=
A2780adr	NIfaWXBHfW6ldHnvckBie3OjeR?=	NU[w[mVTOTBidV2=	Ml;5N\|AhdWmwcx?=	MWLJcohq[mm2aX;uJI9nKGi3bXHuJHAu\3Bib4\ldoV5eHKnc4Pl[EBqdiCmb4jvdpVjcWOrbjDy[ZNqe3SjboSgRVI4QDCjZIKgZ4VtdHNiYYSgNVAhfU1icILlbY5kfWKjdHXkJIZweiB\|MDDtbY5{KHC{aX;yJJRwKGOjbHPlbY4hSU1iYXTkbZRqd25ibXXhd5Vz\WRiZY\ldpkhPjBic3Xjd{B2eCC2bzC5NEBucW6\|IHL5JINidGOnaX6gRW0h[WOldX31cIF1cW:wIHHzd4F6Ng>?	NEC5VGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxO\|g5QCd-MkOwNVc5QDh:L3G+
HL60	NYSwbJlnTnWwY4Tpc44h[XO\|YYm=	NGC1d2I2KHWP	MnTNOFAhdWmwcx?=	MorhTY5pcWKrdHnvckBw\iCFYUKrMYlv\HWlZXSgcYl1d2Oqb37kdolidCCyZYLt[YFjcWyrdImgeJJidnOrdHnvckBqdiCqdX3hckBJVDZyIHPlcIx{KGG2IEWgeW0h[W[2ZYKgOFAhdWmwczDy[YxifGm4ZTD0c{Bkd262cn;sMi=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{NkC4N{c,OjNyMk[wPFM9N2F-
RPMI1788	M3;ndGludXWwb4P1dJBz\XO\|aY\lJIF{e2G7		NH2xVo43KGSjeYO=	M2O4Z2ludXWwb4P1dJBz\XO\|aY\lJIFkfGm4aYT5JIlvKGi3bXHuJHJRVUlzN{i4JINmdGy\|IHPvZ5VtfHW{ZXSge4l1cCCqdX3hckBRSk2FIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiW{PIYZRpgW2rZHnu[UBqdmOxcoDvdoF1cW:wIHnuZ5Vj[XSnZDDmc5IhPiCmYYnzJJBzcW:{IITvJHs{UF22aIntbYRqdmViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOThiaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDJR\|UxKD1iMD6wOVQh\|ryPLh?=	MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NEe4NFQoRjJ\|M{S3PFA1RC:jPh?=
MT4	NIe0O3JCdnSrdnnyZYwh[XO\|YYm=		M3XXeVQh\GG7cx?=	NVTjUItqSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[2:rbn\lZ5Rm\CC5aYToJGhVVFZzIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYL1d{1qdmS3Y3XkJIN6fG:yYYToc4dmdmmlaYT5JIFnfGW{IESg[IF6eyCkeTDNWHQh[XO\|YYmsJGVEPTBiPTCwMlQ2KM7:TT6=	NXnCe25nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFk5QDBpPkKzPFQ6QDhyPD;hQi=>
A2780Adr	MlLkSpVv[3Srb36gZZN{[Xl?		M2P0flMxKG2rboO=	NIS3WVJKdmirYnn0bY9vKG:oIICt[4x6[2:ycn;0[YlvKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEF{N{iwRYRzKGOnbHzzJIF{e2W\|c3XkJIF{KGOjbHPlbY4uSU1iYXPjeY12dGG2aX;uJJBz\WmwY4XiZZRm\CCob4KgN\|AhdWmwczDi[YZwemViY3HsZ4Vqdi2DTTDh[IRqfGmxbjDt[YF{fXKnZDD1dEB1dyB7MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuPVkh\|ryPLh?=	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh3MUGxOEc,OjN6NUGxNVQ9N2F-
2008	MoDFSpVv[3Srb36gZZN{[Xl?		M2r5XVMxKG2rboO=	MnLDTY5pcWKrdHnvckBw\iCPUmCxJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKGi3bXHuJFIxODhiY3XscJMh[XO\|ZYPz[YQh[XNiY3HsZ4Vqdi2DTTDhZ4N2dXWuYYTpc44heHKnaX7jeYJifGWmIH\vdkA{OCCvaX7zJIJm\m:{ZTDjZYxk\WmwLVHNJIFl\Gm2aX;uJI1m[XO3cnXkJJVxKHSxIEmwJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBKSzVyIE2gNk45OSEQvF2u	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh3MUGxOEc,OjN6NUGxNVQ9N2F-
Jurkat T	MVjGeY5kfGmxbjDhd5NigQ>?		MYGzNEBucW6\|	NE\HN2pKdmirYnn0bY9vKG:oIF7GRXQh[WO2aY\heIlwdiCrbjDQUWEwcW:wb335Z4lvNXO2aX31cIF1\WRiaIXtZY4hUnW{a3H0JHQh[2WubIOgbY5kfWKjdHXkJIZweiB\|MDDtbY5{KHC{aX;yJJRwKFCPQT;pc45wdXmlaX6gd5RqdXWuYYTpc44hdWWjc4Xy[YQh[W[2ZYKgOUBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yMEG0JO69VS5?	NF;FTHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2OFk6OSd-MkO5OlQ6QTF:L3G+
MDA435/LCC6MDR	MXPGeY5kfGmxbjDhd5NigQ>?			NG[2TpVOd2S3bHH0bY9vKG:oIICt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N\|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPhcEBw\iCyYXPsbZRigGWuIILld4l{fGGwY3WsJGVEPTBiPTCwMlA{OiEQvF2u	M1f4WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUexOFc5Lz5{NEG3NVQ4QDxxYU6=
MDA435/LCC6	M1S1R2N6fG:2b4jpZ4l1gSCjc4PhfS=>		M3X4N\|Mh\GG7cx?=	NFvpOXhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEF2M{WvUGNEPiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA{KGSjeYOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOi56IN88UU4>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5MUS3PEc,OjRzN{G0O\|g9N2F-
MDA435/LCC6MDR	MkW0R5l1d3SxeHnjbZR6KGG\|c3H5		M3X6OlMh\GG7cx?=	NXi4fJp{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMzDkZZl{KGK7IF3UV{Bie3OjeTygTWM2OCB;IEiuN{DPxE1w	MnXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{G0O\|goRjJ2MUexOFc5RC:jPh?=
L929	MXnDfZRwfG:6aXPpeJkh[XO\|YYm=		NYn3ZpB2OyCmYYnz	MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W\|ZTDMPVI6KGOnbHzzJIF{e2W\|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFMh\GG7czDifUBOXFNiYYPzZZktKEmFNUCgQUA{Oy57IN88UU4>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5MUS3PEc,OjRzN{G0O\|g9N2F-
A2780adr	MULGeY5kfGmxbjDhd5NigQ>?		NFrWTlh2eCC2bzC5NEBucW6\|	M4PKS2lvcGmkaYTpc44hd2ZiTVTSNUBqdiCqdX3hckBld3ixcoXibYNqdi2{ZYPpd5RidnRiQUK3PFBi\HJiY3XscJMh[XO\|ZYPz[YQh[XNiQ3HsZ4VqdiCDTTDhZ4N2dXWuYYTpc44hfHKnYYTl[EA{OCCvaX7zJIJm\m:{ZTDDZYxk\WmwIFHNJIFl\Gm2aX;uJI1m[XO3cnXkJJVxKHSxIEmwJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBKSzVyIE2gNE46OiEQvF2u	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NEKxN{c,OjRzOESyNVM9N2F-
2008	MW\GeY5kfGmxbjDhd5NigQ>?		M4CzdZVxKHSxIEmwJI1qdnN?	NIT3[VJKdmirYnn0bY9vKG:oIF3SVFEhcW5iaIXtZY4hOjByODDj[YxteyCjc4Pld5Nm\CCjczDDZYxk\WmwIFHNJIFk[3WvdXzheIlwdiC2cnXheIVlKDNyIH3pcpMh[mWob4LlJGNidGOnaX6gRW0h[WSmaYTpc44hdWWjc4Xy[YQhfXBidH:gPVAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzNjdizszNMi=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NEKxN{c,OjRzOESyNVM9N2F-
Saos2	M4C5d2Z2dmO2aX;uJIF{e2G7	Ml31OUB2VQ>?	MnXXN{BpenN?	M1n1eWlvcGmkaYTpc44hd2ZicD3ndEBqdiCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iU3Hvd\|Ih[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iZH;4c5J2[mmlaX6geZB1[WunIHH0JFUhfU1iYX\0[ZIhOyCqcoOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMi=>	MoHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MkC0OVIoRjJ2N{KwOFUzRC:jPh?=
Saos2	NWW4XoFpTnWwY4Tpc44h[XO\|YYm=	NGTtdoc2KHWP	M1z0S\|IhcHK\|	NG\1XGZKdmirYnn0bY9vKG:oIICt[5AhcW5iZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIGPhc5MzKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh\G:6b4L1ZolkcW5icnX0[Y51cW:wIHH0JFUhfU1iYX\0[ZIhOiCqcoOgZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nzMi=>	M3PBd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{KwOFUzLz5{NEeyNFQ2OjxxYU6=
Saos2	MV;GeY5kfGmxbjDhd5NigQ>?	MoTmOUB2VQ>?	NID2SIEzKHSxIEOgbJJ{	NVzyfot{UW6qaXLpeIlwdiCxZjDwMYdxKGmwIHTvfI9zfWKrY3nuMZJme2m\|dHHueEBpfW2jbjDTZY9{OiClZXzsd{Bie3Onc4Pl[EBieyCrboTyZYNmdGy3bHHyJIRwgG:{dXLpZ4lvKGSrc4TybYJ2fGmxbjDpckBvfWOuZXmgZZQhPSC3TTDh[pRmeiB{IITvJFMhcHK\|IHL5JIxie2W{IIPjZY5vcW6pIHPvcoZw[2GuIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzMi=>	M4DF[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{KwOFUzLz5{NEeyNFQ2OjxxYU6=
mouse	MnzDSpVv[3Srb36gZZN{[Xl?			MlnQTY5pcWKrdHnvckBw\iCqdX3hckBOTFJzIHX4dJJme3OnZDDpckBud3W\|ZTDMOVE4QCClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDv[kBzcG:mYX3pcoUhOTJ\|IHL5JGZCS1OFYXzpZpVzKG[ub4egZ5l1d22ndIL5MEBKSzVyIE2gNE43PyEQvF2u	Mn\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyNlYoRjJ2OUCwNlI3RC:jPh?=
A2780	NHr3T\|RHfW6ldHnvckBie3OjeR?=	M{DwclUhfU1?	M3XHRlQxKG2rboO=	Mnm3TY5pcWKrdHnvckBw\iCSZ4CtcYVlcWG2ZXSg[JJ2\yCnZn\seZghcW5iYXTybYFugWOrbj3y[ZNqe3SjboSgbJVu[W5iQUK3PFAh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBi\HKrYX35Z4lvKGyndnXsJIF1KDVidV2gZYZ1\XJiNECgcYlveyCkeTDmcI94KGO7dH;t[ZRzgSCjbnHsfZNqey5?	M2\mZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwN\|I{Lz5{NEmwNFMzOzxxYU6=
A2780	NVnkSXl{TnWwY4Tpc44h[XO\|YYm=	MYW1JJVO	NH7tU2cyKGi{	NWPrdm9qUW6qaXLpeIlwdiCxZjDQ[5AudWWmaXH0[YQh\HK3ZzDl[oZtfXhiaX6gZYRzcWGveXPpck1z\XOrc4ThcpQhcHWvYX6gRVI4QDBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYRzcWGveXPpckB2eHSja3WgZZQhPSC3TTDh[pRmeiBzIHjyJIJ6KG[ub4egZ5l1d22ndIL5JIFv[Wy7c3nzMi=>	MmW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECzNlMoRjJ2OUCwN\|I{RC:jPh?=
A2780	MWLGeY5kfGmxbjDhd5NigQ>?	MleyOUB2VQ>?	NGPuc4k5KGi{cx?=	MkjKTY5pcWKrdHnvckBw\iCSZ4CtcYVlcWG2ZXSg[JJ2\yCnZn\seZghcW5iYXTybYFugWOrbj3y[ZNqe3SjboSgbJVu[W5iQUK3PFAh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXTybYFugWOrbjD1dJRic2ViYYSgOUB2VSCjZoTldkA5KGi{czDifUBndG:5IHP5eI9u\XS{eTDhcoFtgXOrcz6=	M2P2b\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwN\|I{Lz5{NEmwNFMzOzxxYU6=
T-cells	NIq4OnVCdnSraX7mcIFudWG2b4L5JIF{e2G7	NYTwWWNFOC5yMTD0c{AyODBibl2=	M4Dke\|Ih\GG7cx?=	NGLHWI1CdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKEyOT{GxPEBVS1JidILhcpNo\W6rYzDtc5V{\SCLTD2yMYNwdXCndHXueEBETDRvcH;zbZRqfmViVDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEyOTzCoNVkxKHSxIEKwOUkucW6mdXPl[EBKVC1{IIDyc4R2[3Srb36gZZQhOC5yMTD0c{AyODBibl2g[o9zKDJiZHH5d{BjgSCobH;3JIN6fG:vZYTybYMh[W6jbInzbZMv	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEi2Okc,OjR7MEC4OlY9N2F-
Th17	NEfvW\|FCdnSraX7mcIFudWG2b4L5JIF{e2G7	MX[xNEB1dyBzMECgcm0>	M330VVIh\GG7cx?=	MmruRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBNVE9zMUigWGNTKHS{YX7z[4VvcWNibX;1d4UhXGhzNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEmOLUG3JJBzd2S3Y4Tpc44h[XRiMUCgeI8hOTByIH7NJIZweiB{IHThfZMh[nliZnzve{BkgXSxbXX0dolkKGGwYXz5d4l{Ng>?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEi2Okc,OjR7MEC4OlY9N2F-
Th1	Mm[1RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>?	NXzxdnpuOTByIH7N	MVWyJIRigXN?	MUfBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJGxNVzFzODDUR3IhfHKjboPn[Y5q[yCvb4Xz[UBVcDFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBKTk5vZ3HtcYEheHKxZIXjeIlwdiCjdDCxNFAhdk1iZn;yJFIh\GG7czDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm\|aYOu	NIPQ[Ik9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFg3Pid-MkS5NFA5PjZ:L3G+
NCI/ADR-RES	NETQOpRHfW6ldHnvckBie3OjeR?=		M{e0PVIxKG2rboO=	MWHJcohq[mm2aX;uJI9nKGi3bXHuJHAu\2y7Y3;wdo91\WmwIHX4dJJme3OnZDDpckBPS0lxQVTSMXJGWyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gZ4Ft[2Wrbj3BUUB1emGwc4DvdpQh[W[2ZYKgNlAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZkv	M3PVZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUmyNVU{Lz5{NEm5NlE2OzxxYU6=
A2780adr	MYfGeY5kfGmxbjDhd5NigQ>?		NInOSJk6OCCvaX7z	MVrJcohq[mm2aX;uJI9nKEGEQ1KxJIlvKGi3bXHuJGEzPzhyYXTyJINmdGy\|IIDy[U1qdmO3YnH0[YQh\m:{IEOwJI1qdnNiZn;scI94\WRiYomgZ4Ft[2WrbjDBUUBi\GSrdHnvckBidmRiZoXyeIhmeiCrbnP1ZoF1\WRiZn;yJFYxKG2rboOgZpkh[2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFAvQTF7IN88UU4>	NV\hOWhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OVU5QTVpPkK1PFU2QDl3PD;hQi=>
T spleen cells	MX;DfZRwfG:6aXPpeJkh[XO\|YYm=		M2DZ[VczKGi{cx?=	NVfBPXFPS3m2b4TvfIlkcXS7IHHnZYlve3RiSVPSJI1wfXOnIGSgd5Bt\WWwIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjBzIN88UU4>	NIS3U4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2OFI3Pid-Mk[1OlQzPjZ:L3G+
B spleen cells	M{KxPWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MUW3NkBpenN?	MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBKS1JibX;1d4UhSiC\|cHzl[Y4h[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFQh\|ryPLh?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV4NEK2Okc,OjZ3NkSyOlY9N2F-
RD	MXnBcpRqfmm{YXygZZN{[Xl?		MYWyOEBpenN?	MUPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6\|dDDFWlcyKGmwZnXjeIVlKGmwIGLEJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOy54NjFOwG0v	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV4NEK2Okc,OjZ3NkSyOlY9N2F-
mouse lymph node cells	M{D2SGZ2dmO2aX;uJIF{e2G7			NWX6fYQ5UW6qaXLpeIlwdiCxZjDQTVNMNWejbX3hJIlvKG2xdYPlJIx6dXCqIH7v[IUh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDDc45CNXO2aX31cIF1\WRiVDDj[YxtKHC{b3zp[oVz[XSrb36sJGlEPTBiPTCwMlU4KM7:TT6=	Mn;hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MkCxOVQoRjJ4N{KwNVU1RC:jPh?=
CD1	MlvFSpVv[3Srb36gZZN{[Xl?	MVywMlEhfG9iMUCgeW0>	NEi0Z4M{OCCvaX7z	NGjUdoRKdmirYnn0bY9vKG:oIITheZJwdGm2aH;jbI9t[XSnIHHjbYQhOy2\|dXzmZZRmNWmwZIXj[YQhdmWlcn;zbZMhcW5iQ1SxJI1wfXOnIIDhcoNz\WG2aXOgZYNqdmG{IHPlcIx{KGG2IECuNUB1dyBzMDD1UUBi\nSncjCzNEBucW6\|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZoF{\WRiY3;u[o9k[WxibXnjdo9{[2:yaXOgZY5idHm\|aYOu	NF7jT5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm1NFM6Oid-Mk[5OVA{QTJ:L3G+
A2780/ADR	MmDESpVv[3Srb36gZZN{[Xl?		MYOzNEBucW6\|	MX7Jcohq[mm2aX;uJI9nKEGEQ1KxJIlvKGi3bXHuJGEzPzhyL1HEVkBk\WyuczDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMhdWWjc4Xy[YQhfXBidH:gN\|YxOCC\|ZXPzJJdqfGhidHnt[UBqdnSnco\hcJMhd2ZiNkCgd4VkeyCkeTDjZYxk\WmwIHHjZ5VufWyjdHnvckBie3OjeTygTWM2OCB;IEGuOFEh\|ryPLh?=	M4PneVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUCwNFM{Lz5{N{GwNFA{OzxxYU6=
A2780/ADR	MlLiR5l1d3SxeHnjbZR6KGG\|c3H5		MXS3NkBpenN?	NG\Lco5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MD;BSHIh[2WubIOgc5ZmemW6cILld5NqdmdiQVLDRlEh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFfJOVAhRSB6Lk[zJO69VS5?	NHWzNZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GwNFA{Oyd-MkexNFAxOzN:L3G+
A2780	NYnKSpc{S3m2b4TvfIlkcXS7IHHzd4F6		NIXEb\|c4OiCqcoO=	MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDhwOUSg{txONg>?	NF71[W89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GwNFA{Oyd-MkexNFAxOzN:L3G+
MDCK2	NY[0Z3A6TnWwY4Tpc44h[XO\|YYm=			NIHFfmFKdmirYnn0bY9vKG:oIFHCR2MzKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ER2szKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4Ft[2Wrbj3BUUBm\m[udYigcYVie3W{ZXSgZYZ1\XJiM{CgcYlveyCrbjDwdoV{\W6lZTDv[kBCSkOEMTDpcohq[mm2b4KgS2YyOjB7MUigZY5lKEGEQ1OxJIlvcGmkaYTvdkBOUy13N{GgZpkh\myxdzDjfZRwdWW2comsJGlEPTBiPTCyOU43KM7:TT6=	NHLsSm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5N\|k1QSd-MkezPVM6PDl:L3G+
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hamster BHK21	MoriSpVv[3Srb36gZZN{[Xl?	NHL0eWszPSC3TR?=	M1G1XlMxKG2rboO=	NVnWOJV[UW6qaXLpeIlwdiCxZjDoeY1idiCDQlPDNUBmgHC{ZYPz[YQhcW5iaHHtd5RmeiCESFuyNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPhcINmcW5vQV2g[YZndHW6IHH0JFI2KHWPIH3lZZN2emWmIHHmeIVzKDNyIH3pcpMh[nliZnzve{BkgXSxbXX0dpkv	NGnmSmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5N\|k1QSd-MkezPVM6PDl:L3G+
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Rh18	Ml\ZdWhVWyCjc4PhfS=>			MY\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtey5?	MmrVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	MYHxTHRUKGG\|c3H5			M1rX[JFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHNw	M1z1cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32	Mne0dWhVWyCjc4PhfS=>			NWe4Z4w{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtey5?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
C57BL/6 mouse lymph node cells	NYjrbYJyUW2vdX7vd5VxeHKnc4PpeoUh[XO\|YYm=		Moj6NlQhcHK\|	M1\YdWludXWwb4P1dJBz\XO\|aY\lJIFkfGm4aYT5JIlvKEN3N1LMM\|YhdW:3c3WgcJlueGhibn;k[UBk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD61JO69VS5?	NV;GXJZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4O\|E5PDRpPkK5PFcyQDR2PD;hQi=>
HuH7	NX63NpN1SW62aY\pdoFtKGG\|c3H5		MoHSN{Bl[Xm\|	M4jOWWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEXiCpZX7veJlx\SB{YTDpcoZm[3SnZDDpckBpfW2jbjDIeWg4KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDkeYFtNWeubzDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjd5MzFOwG0v	NYHneo9oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwO\|Q4QTVpPkOwNFc1Pzl3PD;hQi=>
MT4	M3L6OmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MXi0JJRwKDViZHH5dy=>	NG[1fIlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXDRiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDRidH:gOUBl[Xm\|IHL5JINmdGxvdHn0[ZIh\2yxIHHzd4F6NCCFQ{WwJF0hPS54MEig{txONg>?	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB5NEe5OUc,OzByN{S3PVU9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; PubMed: 12644839 Br J Cancer. Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm). Cyp-D / AIF / β-actin; PubMed: 29328412 Mol Med Rep. Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. P<0.05, P<0.01, **P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor. IL-8 / MCP-1; PubMed: 9602633 Br J Ophthalmol. Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity. Actin / Smac; PubMed: 12427831 J Neurosci. Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release. β-actin / FIPV-N; PubMed: 22546085 Vet Res. Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.	12644839 29328412 9602633 12427831 22546085
Growth inhibition assay	NPC death; PubMed: 20181586 J Neurosci. Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).	20181586
IHC	Th22 / Th17 / Th1 / M1 / Th2; PubMed: 30916387 Clin Exp Immunol. (a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. Tregs / Bregs / pDCs / M2; PubMed: 30916387 Clin Exp Immunol. (b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. Ki 67 / Fas-FasL; PubMed: 30916387 Clin Exp Immunol. (c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method. IFN-γ / TNF-α; PubMed: 10931158 Clin Exp Immunol. Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat. WT-PCLS / FXRKO-PCLS; PubMed: 26482353 BMC Genomics. Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).	30916387 10931158 26482353
Immunofluorescence	Human gingival fibroblast cells; PubMed: 27857765 Dent Res J (Isfahan). Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole. Human gingival fibroblast cells; PubMed: 27857765 Dent Res J (Isfahan). Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts. Calcein; PubMed: 23593196 PLoS One. E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm). Rhod-Dex / Calcein; PubMed: 20584761 Toxicol Sci. Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM). cadherin-9 / vimentin; PubMed: 17668045 PLoS One. To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.	27857765 23593196 20584761 17668045

体内研究

Cyclosporin A是一种免疫抑制剂，小鼠，大鼠，和荷兰猪经非肠道给药或者口服给药都有效^[6]，临床上用于器官移植中预防免疫排斥。^[7]

激酶实验：^[3]	- 合并磷酸酶试验: 纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶，100 nM 钙调蛋白，以及5 μM ³²P-标记磷酸化多肽，60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8)，100 mM NaCI，6 mM MgCl₂，0.5 mM 二硫苏糖醇，每毫升0.1毫克牛血清白蛋白，以及0.1 mM CaCl₂或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物， 5 μM ³²P-标记的磷酸肽，和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸，一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca²⁺-不依赖性磷酸酶，而更高浓度时也会部分抑制Ca²⁺-依赖性的活性。30°C下培育15分钟，加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐，用闪烁计数测定数量。
细胞实验：^[3]	- 合并 Cell lines: Jurkat细胞(无性繁殖J77) Concentrations: ~100 nM Incubation Time: 1小时 Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(10⁶)悬浮于微量离心管1ml完全培养基中；1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中，细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次，在50μl低渗缓冲液中裂解，缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯，50 微克胰蛋白酶抑制剂，5微克亮抑酶肽，和5 微克抑肽酶。裂解液用液氮冷冻，再在30°C下融化，循环3次，随后在 4°C下以12,000×g离心10分钟。 (Only for Reference)
动物实验：^[6]	- 合并 Animal Models: 大鼠 Dosages: 45毫克/千克 Administration: 口服 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	100 mg/mL warmed (83.15 mM)
	Water	Insoluble
	Ethanol	''''100 mg/mL warmed
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+5% Tween 80+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Cyclosporin A (NSC 290193) SDF

分子量	1202.61
化学式	C₆₂H₁₁₁N₁₁O₁₂
CAS号	59865-13-3
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	Cyclosporine A, Cyclosporine, Ciclosporin, CsA

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04989686	Not yet recruiting	Other: Microsampling	Immunosuppression	Children''s Hospital of Philadelphia	October 1 2021	--
NCT04023760	Completed	Drug: Apixaban	Pharmacokinetics\|Kidney Transplant\|Lung Transplant	University of Saskatchewan\|Saskatchewan Health Research Foundation\|Lung Association of Saskatchewan	June 26 2019	Phase 4
NCT03865888	Completed	Drug: Cyclosporins\|Drug: Tacrolimus	Dry Eye\|Sjogren Syndrome	Pavly Moawd\|Cairo University	October 30 2018	Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?
回答：
Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

研究领域

产品类型

定制您的化合物库

Cyclosporin A (NSC 290193)

客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献54篇：

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Cyclosporin A (NSC 290193) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

技术支持

常见问题及建议解决方法