Cyclosporin A (NSC 290193)

For research use only. Not for use in humans.

目录号:S2286 别名: Cyclosporine A, Cyclosporine, Ciclosporin, CsA 中文名称:环孢菌素A

Cyclosporin A (NSC 290193) Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1073.15 现货
RMB 786.07 现货
RMB 1957.41 现货
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客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献54篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。
靶点
calcineurin [1]
(Cell-free assay)
7 nM
体外研究

在T细胞中,Cyclosporin A与亲环蛋白(免疫亲和素)结合[1],形成亲环蛋白-Cyclosporin A复合体,其结合并抑制钙调磷酸酶。[2]Cyclosporin A 抑制钙调磷酸酶,IC50 是7 nM[3],随后阻断NF-AT核转运。 [4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体,IC50 是39 nM。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells MkflR5l1d3SxeHnjxsBie3OjeR?= NUfNU4ltS3m2b4TvfIlkcXS7IHHnZYlve3RicnH0JGg1OkViY3XscJMh[XO|ZYPz[YQh[XNiaX70doFk\WyudXzhdkBCXFBibHX2[YwtKFSFNUC9OFIh|ryP M2H6ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUCyNFU5Lz5zOUWwNlA2QDxxYU6=
mouse L929 cells NGHFRYtEgXSxdH;4bYPDqGG|c3H5 NFzE[Ww{KGSjeYO= Mo\OR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFkzQSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRUKGG|c3H5MEBKSzVyPUOwJO69VQ>? MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEWxPVUoRjJ3OUi1NVk2RC:jPh?=
human Huh-Mono cells NUG5fGlDS3m2b4TvfIlkyqCjc4PhfS=> NEHUbHY{KGSjeYO= MoDaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNU2xbn:gZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5Nige,:jDDDR|UxRTJ2IN88US=> MoOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O|Y3RC:jPh?=
MDCK cells M3S2XWN6fG:2b4jpZ:Kh[XO|YYm= M{foPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQ1ugZ4VtdHNuIGTDOVA:QS57IN88US=> MmG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells Ml\vR5l1d3SxeHnjxsBie3OjeR?= MXy3NkBp MlH3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUGNENTZiY3XscJMh[W[2ZYKgO|IhcHK|LDDJR|UxRThwMzFOwG0> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ|MkC0NFI9N2F-
human HeLa cells NEHlVXpEgXSxdH;4bYPDqGG|c3H5 NYTVbHA1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyxiVFO1NF05NjNizszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
african green monkey Vero cells NH21W21HfW6ldHnvckBie3OjeR?= M1jqNFI1KGh? Ml3sRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fCCQaXzlJJZqenW|IHnu[oVkfGWmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KF[ncn:gZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfmm{YXygVm5CKHO7boTo[ZNqeyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRThizszN MkjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGyPFYoRjF7NEWxNlg3RC:jPh?=
human Huh-9-13 cells MWLDfZRwfG:6aXRCpIF{e2G7 NXfQcYJqOyCmYYnz NXPwbpVtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqLUmtNVMh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3Ojef-8kEBESzVyPUeg{txO MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW3OlYoRjF6NkK1O|Y3RC:jPh?=
human HuH6 cells MmLuR5l1d3SxeHnjxsBie3OjeR?= M3\heVMh\GG7cx?= NIXnPIhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh4IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEOFNUC9O{DPxE1? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human Huh5-2 cells Mo\oR5l1d3SxeHnjxsBie3OjeR?= NHjUd3I{KGSjeYO= MnHBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPS1{IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZkhNCCFQ{WwQVYh|ryP M{jsV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH7 cells MofzR5l1d3SxeHnjxsBie3OjeR?= NWDJNVRRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[Yxm[XOnIH;mJIxi[3SjdHWg[IVpgWS{b3flcoF{\SxiSVO1NF02NjhzIN88US=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl2M{O5NEc,OjB7NEOzPVA9N2F-
human 2008 cells M4jrTmZ2dmO2aX;uJIF{e2G7 M2S4UGlvcGmkaYTpc44hd2ZiaIXtZY4hVVKSMTDpckBpfW2jbjCyNFA5KGOnbHzzMEBKSzVyPUSuO|gh|ryP NGLtbJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEewO|g5PCd-MUi3NFc5QDR:L3G+
human HeLa cells M3fRZWZ2dmO2aX;uJIF{e2G7 NITUSoVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJWFZvMUigbY5n\WO2ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEeg[IF6e+,:jDDUR|UxRTRwNjFOwG0> NHrW[JM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
leukemia CEM cells MofxSpVv[3Srb36gZZN{[Xl? NV\Jd5FTUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEi3bXHuJG1FWjFiUD3HcJlkd3C{b4TlbY4hSUKFIGTyZY5{eG:{dHXyJJV{cW6pIHzleYtmdWmjIFPFUUBk\WyuczygTWM2OD1|LkSg{txO M3\4UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN|g4Lz5zMkO2NVM5PzxxYU6=
human Caco2 cells NELTN2tHfW6ldHnvckBie3OjeR?= M{nPOY5pcWKrdHnvckBw\iCSLXfwJIlvKGi3bXHuJGNi[29{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBlcWexeHnuJIVn\my3eDygTWM2OD1{Lkmg{txO MkDPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OEK0OVgoRjJ|M{iyOFU5RC:jPh?=
human MDA435/LCC6MDR cells NEPqSpdEgXSxdH;4bYPDqGG|c3H5 MWG3Nog> Mn3kR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=> M1zZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KwOFAzLz5{MkOyNFQxOjxxYU6=
guinea pig ventricular myocytes Mne2SpVv[3Srb36gZZN{[Xl? M2raSWlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1? NIrySYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2NVAxOCd-MkK3OlExODB:L3G+
KB/MDR cell MofESpVv[3Srb36gZZN{[Xl? M3LUcVczKGh? Moq5TY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDN02GUjDj[YxtKGyrbnWgZYZ1\XJiN{KgbJIhd2ZiZIL1[{BmgHCxc4Xy[UwhUUN3ME2xMlUh|ryP MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MUm5NUc,OTV2OEG5PVE9N2F-
A2780/ADR cells MnLDSpVv[3Srb36gZZN{[Xl? M1T6S2lvcGmkaYTpc44hd2ZiUD3ncJlkd3C{b4TlbY4h\XiycnXzd4VlKGmwIFGyO|gxN0GGUjDj[YxteyCkeTDjZYxk\WmwIFHNJIF{e2G778{MJGlEPTB;MT60NVI2PCEQvF2= NXGyc|VTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells NW\lSGVWTnWwY4Tpc44h[XO|YYm= NITVZmgyKM7:TR?= NF76b5Y4OiCq NXu0ZVFRWG:2ZX70bYF1cW:wIH;mJIRwgG:{dXLpZ4lvNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIwWjdiY3XscJMh[XO|ZYPz[YQh[XNiZH;4c5J2[mmlaX6gTWM2OCCjdDCxJJVOKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE44KM7:TR?= MnnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{SwOFEoRjJ3NkO0NFQyRC:jPh?=
LLC-PK1 epithelial cells M1fySmZ2dmO2aX;uJIF{e2G7 NET5bWdKdmirYnn0bY9vKG:oIGCt[4x6[2:ycn;0[YlvNCCvb4Xz[UBNNW2mckHiJIV5eHKnc4Pl[EBqdiCOTFOtVGsyKGWyaYTo[Yxq[WxiY3XscJMhfXOrbnegZ4Ft[2Wrbj3BUUBxd2yjcnnzZZRqd25iYYPzZZnwxIxiSVO1NF0xNjdizszN M3rRe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{Nkm5N|g6Lz5zMk[5PVM5QTxxYU6=
mouse L5178 cells NF\qfJNHfW6ldHnvckBie3OjeR?= NV7KNHo4OjBibXnudy=> NE[1W3FKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1KxMY1m\GmjdHXkJJJpd2SjbXnu[UAyOjNiZX\mcJV5KGmwIH3veZNmKEx3MUe4JINmdGy|IHX4dJJme3OrbnegbJVu[W5iTVTSNUBi\nSncjCyNEBucW6|IHL5JGZCS1NiYX7hcJl{cXNuIFnDOVA:OC54NTFOwG0> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2MUe0OUc,OjF|NEG3OFU9N2F-
KB cell MX3GeY5kfGmxbjDhd5NigQ>? M3fT[2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBMSiClZXzsJIxqdmViYX\0[ZIhPzJiaDDv[kBlenWpIHX4dI9{fXKnLDDJR|UxRTBwNjFOwG0> M3j0S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
mouse mammary carcinoma cell line EMT6/AR 1.0 NV25NZVYTnWwY4Tpc44h[XO|YYm= MY\S[ZZmenOjbDDl[oZm[3Rib36geIhmKGGlY4XteYxifGmxbjDv[kBcO0ifLTDkZZVvd3K3YnnjbY4hcW5ibX;1d4UhdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mKEWPVE[vRXIhOS5yLDDJR|UxRTBwNESg{txO NWTOVIgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwPVg3PzFpPkGwNFk5PjdzPD;hQi=>
CK1 cells M37hUmZ2dmO2aX;uJIF{e2G7 NUfDU2RvSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUOzJIlv\mWldHXkJIlvKEONMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFch\GG7czygWGM2OD1yLkOg{txO M4LoNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
HEK293 cells NF[yVWFHfW6ldHnvckBie3OjeR?= MmnJTY5pcWKrdHnvckBw\iCRQWTQNWIyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCycn;0[YlvNW2nZHnheIVlKHCrdHH2ZZN1[XSrbjD1dJRic2YxvJygT4k:OC5{IN88US=> MkHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OEe5PFYoRjJ{NUi3PVg3RC:jPh?=
human MDA435/LCC6MDR cells NEP1NmJHfW6ldHnvckBie3OjeR?= NYH5UnpVPSCmYYnz NH7CTFlOd2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpcocheGGlbHn0ZZhmdCC{ZYPpd5RidmOnIH3lZZN2emWmIHHzJINmdGxic4Xyeol3[WxiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5NigSxiRVO1NF0xNjB|MjFOwG0v M{jieFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC MkH0VJJwdGmoZYLheIlwdiCjc4PhfS=> NFq1bHNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDCUWMtKEmFNUC9NVIhdk1? NIjlbVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[3PVMxQSd-MUW2O|k{ODl:L3G+
mouse T cells M4PMTGZ2dmO2aX;uJIF{e2G7 M2DzOVQh\GG7cx?= NWfUXnlqUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5ibX;1d4UhXCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJR4dyC5YYmgcYl5\WRibIntdIhw[3m2ZTDy[YFkfGmxbjDh[pRmeiB2IHThfZMh[nliTWTUJIF{e2G7LDDpZ|UxRTFzIH7N MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6NEm4O{c,OjJ7OES5PFc9N2F-
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MDA-435/LCC6 and MDA-435/LCC6-MDRI NYPiWldoTnWwY4Tpc44h[XO|YYm= NIDQbnZEd26lZX70doF1cW:wIIToZZQhemWmdXPld{BlcW[oZYLlcoNmKGmwIILleoVze2GuIH;mJHs{UF2YQlygZYNkfW23bHH0bY9vKGKndIfl[Y4hVUSDLUSzOU9NS0N4IHHu[EBOTEFvNEO1M2xESzZvTVTSTUBk\WyuczDifUA2OCVuIFXDOVAhRSByLk[g{txONg>? M1[2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
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Jurkat NVG1NHhHTnWwY4Tpc44h[XO|YYm= M2ToZWlvcGmkaYTpc44hd2ZicILv[JVkfGmxbjDv[kBj\XSjLXfhcIFkfG:|aXThd4UhUUxvMjDSS2EhcW5iSoXyb4F1KGOnbHzzMEBKSzVyIE2gNE4xODhizszNMi=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTh6MUm4OEc,OTF6OEG5PFQ9N2F-
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High Five cells NI\ZUZdHfW6ldHnvckBie3OjeR?= NWLzToVVcW6qaXLpeIlwdiCxZjDUZZVzd2OqZX7v[IVwgHmlaH;sZZRmKHWydHHr[UhEe0FiM{D1UUwhOzBiJTDv[kBkd262cn;sLUBqdiCvZX3idoFv\SC4ZYPpZ4xmeyCycnXwZZJm\CCocn;tJGhq\2hiRnn2[UBk\WyuczDpcoZm[3SnZDD3bZRpKHSqZTDBRmNDOTFiYnHjeYxwfmm{dYOsJGtqKD1iOT61JO69VS5? MknsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MESyN|koRjF{NEC0NlM6RC:jPh?=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3; 

PubMed: 12644839     


Western blot analysis of phospho-AKT1, AKT1, PTEN, phospho-BAD, BAD and procaspase-3 expression in HepG2 cells treated with cyclosporin A (CA, 10 μm) and/or Taxol (Tx, 0.1 μm).

Cyp-D / AIF / β-actin; 

PubMed: 29328412     


Western blot analysis of protein expression levels of Cyp-D and AIF in the spinal cord. *P<0.05, **P<0.01, ***P<0.001. SCI, spinal cord injury; CsA, cyclosporin A; Cyp-D, cyclophilin-D; AIF, apoptosis-inducing factor.

IL-8 / MCP-1; 

PubMed: 9602633     


Representative northern blots revealing the effects of dexamethasone (DEX) and cyclosporin A (CSA) on rIL-1β induced (20 ng/ml) IL-8 and MCP-1 mRNA expression in human orbital fibroblasts at 24 hours. Equivalent total cellular RNA loading per lane is demonstrated by the electrophoretic profile (below) of 18s and 28s rRNA in all lanes, except the high dose CSA lane in which cell death occurred from cyclosporin toxicity.

Actin / Smac; 

PubMed: 12427831     


Regulation of Aβ25–35-induced Smac release from mitochondria in CECs. CECs were treated with 25 μm Aβ25–35 in the presence of cyclosporin A (B) at indicated concentrations, and cytosolic fractions were analyzed by immunoblotting with anti-Smac antibody. Cyclosporin A inhibited Smac release.

β-actin / FIPV-N; 

PubMed: 22546085     


Cyclosporin A suppresses FIPV replication in fcwf-4 cells. (B) Western blots of cells incubated with various concentrations of CsA. Lanes show total cellular proteins resolved by electrophoresis. Effects of CsA were assessed using monoclonal anti-FIPV-N and anti-β-actin primary antibodies.

12644839 29328412 9602633 12427831 22546085
Growth inhibition assay
NPC death; 

PubMed: 20181586     


Cyclosporin A (CsA) enhanced NPC survival. A, Significantly fewer cyclosporin A-treated NPCs die over 3 d compared with control NPCs (p < 0.05).

20181586
IHC
Th22 / Th17 / Th1 / M1 / Th2; 

PubMed: 30916387     


(a) Percentage of pro‐ and anti‐inflammatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. Interleukin (IL)‐22‐, CD4/IL‐17A‐, CD4/interferon (IFN)‐γ‐, CD16/tumor necrosis factor (TNF)‐α‐ and CD4/IL‐4‐expressing cells in graft biopsies. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD4+ T cell (in brown). Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Tregs / Bregs / pDCs / M2; 

PubMed: 30916387     


(b) Percentage of regulatory cells in graft biopsies from kidney transplant recipients (KTR) under belatacept or cyclosporin A treatment. CD25/forkhead box protein 3 (FoxP3)‐, CD20/IL‐10‐, CD123/L‐kynurenine‐ and CD163/IL‐10‐expressing cells in graft biopsies. Arrows depict double immunoreactive cells (in burgundy). Arrowhead shows a CD25+ T cell (in pink). Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line), and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

Ki 67 / Fas-FasL; 

PubMed: 30916387     


(c) Percentage of senescent, apoptotic and proliferating cells in graft biopsies from KTR under belatacept or cyclosporin A treatment. Ki‐67‐, p16INK4α‐ and FAS/FASL‐expressing cells in graft biopsies. Photomicrographs on the left shows the expression of Ki67. Photomicrographs on the right depict the apoptotic cells. Belatacept: n = 23 biopsies and cyclosporin A: n = 10 biopsies. Results are expressed as median (black line), mean (red line) and 5th/95th percentiles. Statistical analysis: one‐way analysis of variance; Dunn’s method.

IFN-γ / TNF-α; 

PubMed: 10931158     


Effect of cyclosporin A (CsA) on expression of IFN-γ and tumour necrosis factor-alpha (TNF-α). (a) Renal expression of IFN-γ in a rat with chronic serum sickness (CSS). (b) IFN-γ in a rat treated with CsA. (c) TNF-α in rat with CSS. (d) TNF-α expression in a CsA-treated rat.

WT-PCLS / FXRKO-PCLS; 

PubMed: 26482353     


Histological analysis of liver slices treated with cyclosporin A (CsA). WT-PCLS (a-d) were cultured for 24 and 48 h in the presence of DMSO (a and c) or 40 μM CsA (b and d). FXRKO-PCLS (e-h) were cultured for 24 and 48 h in the presence of DMSO (e and g) or 40 μM CsA (f and h).

30916387 10931158 26482353
Immunofluorescence
Human gingival fibroblast cells; 

PubMed: 27857765     


Human gingival fibroblast cells were incubated with calcium green 1 AM for 1 h and cyclosporin A (25 μM) and angiotensin II (100 μM) were added and nuclei stained with 4, 6diamidino-2-phenylindole (blue), imaging was done using an immunofluorescence microscopy under x10 (a). Time-dependent calcium intensity was measured (b). CsA: Cyclosporin A; Ang II: Angiotensin II; DAPI: 4, 6-diamidino-2-phenylindole.

Human gingival fibroblast cells; 

PubMed: 27857765     


Live staining was done by incubating the human gingival fibroblast cells with calcium green 1 AM for 1 h following which cyclosporin A and angiotensin II was added and images were taken using confocal microscope under × 20 objectives (a). Histogram represents the mean fluorescence intensity (b). CsA: Cyclosporin A; Ang II: Angiotensin II; HGF: Human gingival fibroblasts.

Calcein; 

PubMed: 23593196     


E. XR9576, verapamil, and cyclosporin A enhanced calcein fluorescence in KB-V1 cells. KB-V1 cells treated with the indicated compounds and 1 µM calcein AM were imaged 1 hour later. The phase-contrast and fluorescent images were merged and displayed (size bar = 100 µm).

Rhod-Dex / Calcein; 

PubMed: 20584761     


Lack of protection by cyclosporin A against acetaminophen-induced lysosomal breakdown and calcein quenching. Mouse hepatocytes were loaded with rhodamine-dextran and calcein, as described in Figure 6 and treated with acetaminophen (AAP) in the presence of fructose plus glycine and cyclosporin A (CsA, 1μM).

cadherin-9 / vimentin; 

PubMed: 17668045     


To induce a transition of renal epithelial cells into a spindle-shaped mesenchymal cell type, the epithelial TK 163 cells were cultured for 48 hours in different concentrations (0, 4.2 and 42 µM) of cyclosporin-A (CsA). EMT could be observed by phase contrast microscopy. Immunofluorescence staining revealed that without CsA no signals for cadherin-9 and only weak signal for vimentin could be observed. Induction of EMT by CsA led to a strong up-regulation of both vimentin and cadherin-9 expression.

27857765 23593196 20584761 17668045
体内研究 Cyclosporin A是一种免疫抑制剂,小鼠,大鼠,和荷兰猪经非肠道给药或者口服给药都有效[6],临床上用于器官移植中预防免疫排斥。[7]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[3]
- 合并

磷酸酶试验:

纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶,100 nM 钙调蛋白,以及5 μM 32P-标记磷酸化多肽,60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8),100 mM NaCI,6 mM MgCl2,0.5 mM 二硫苏糖醇, 每毫升0.1毫克牛血清白蛋白,以及0.1 mM CaCl2或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物, 5 μM 32P-标记的磷酸肽,和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸,一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca2+-不依赖性磷酸酶,而更高浓度时也会部分抑制Ca2+-依赖性的活性。30°C下培育15分钟,加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐,用闪烁计数测定数量。
细胞实验:[3]
- 合并
  • Cell lines: Jurkat细胞(无性繁殖J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1小时
  • Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(106)悬浮于微量离心管1ml完全培养基中;1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中,细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次,在50μl低渗缓冲液中裂解,缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯,50 微克胰蛋白酶抑制剂,5微克亮抑酶肽,和5 微克抑肽酶。裂解液用液氮冷冻,再在30°C下融化,循环3次,随后在 4°C下以12,000×g离心10分钟。
    (Only for Reference)
动物实验:[6]
- 合并
  • Animal Models: 大鼠
  • Dosages: 45毫克/千克
  • Administration: 口服
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL warmed (83.15 mM)
Water Insoluble
Ethanol ''''100 mg/mL warmed
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 1202.61
化学式

C62H111N11O12

CAS号 59865-13-3
储存条件 粉状
溶于溶剂
别名 Cyclosporine A, Cyclosporine, Ciclosporin, CsA

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04989686 Not yet recruiting Other: Microsampling Immunosuppression Children''s Hospital of Philadelphia October 1 2021 --
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • 回答:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID