Cyclosporin A (NSC 290193)

目录号:S2286 别名: Cyclosporine A, Cyclosporine, Ciclosporin, CsA 中文名称:环孢菌素A

仅限科研使用

Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。

Cyclosporin A (NSC 290193) Chemical Structure

CAS: 59865-13-3

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1073.15 现货
RMB 786.07 现货
RMB 1957.41 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

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客户使用Selleck生产的Cyclosporin A (NSC 290193)发表文献66篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Cyclosporin A (NSC 290193, Cyclosporine A, Cyclosporine, Ciclosporin, CsA)是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。
靶点
calcineurin [1]
(Cell-free assay)
7 nM
体外研究

在T细胞中,Cyclosporin A与亲环蛋白(免疫亲和素)结合[1],形成亲环蛋白-Cyclosporin A复合体,其结合并抑制钙调磷酸酶。[2]Cyclosporin A 抑制钙调磷酸酶,IC50 是7 nM[3],随后阻断NF-AT核转运。 [4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体,IC50 是39 nM。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells M4LIcWN6fG:2b4jpZ:Kh[XO|YYm= M3HwRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHKjdDDIOFJGKGOnbHzzJIF{e2W|c3XkJIF{KGmwdILhZ4VtdHWuYYKgRXRRKGyndnXsMEBVSzVyPUSyJO69VQ>? M4W1fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUCyNFU5Lz5zOUWwNlA2QDxxYU6=
mouse L929 cells MYDDfZRwfG:6aXRCpIF{e2G7 NHnKT3o{KGSjeYO= NFTMPIZEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNQTJ7IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFNiYYPzZZktKEmFNUC9N|Ah|ryP M1HWT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human Huh-Mono cells Mn\UR5l1d3SxeHnjxsBie3OjeR?= MoXkN{Bl[Xm| NGDBVGdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvTX;uc{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;MkSg{txO M4TBdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
MDCK cells NWXKPGdMS3m2b4TvfIlkyqCjc4PhfS=> MkfoR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSFSzDj[YxteyxiVFO1NF06NjlizszN NHPlT5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
human LCC-6 cells MYjDfZRwfG:6aXRCpIF{e2G7 NUnEU3ZjPzJiaB?= MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMR2MuPiClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:QC5|IN88US=> MnzRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MkC0NFIoRjJ{M{KwOFAzRC:jPh?=
human HeLa cells M2\5PGN6fG:2b4jpZ:Kh[XO|YYm= NIG0e3VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{NCCWQ{WwQVgvOyEQvF2= M{fzXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells M3rF[mZ2dmO2aX;uJIF{e2G7 MUGyOEBp MYTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDX[ZN1KE6rbHWgeolzfXNiaX7m[YN1\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhXmW{bzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckB3cXKjbDDSUmEhe3mwdHjld4l{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:QCEQvF2= MoCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGyPFYoRjF7NEWxNlg3RC:jPh?=
human Huh-9-13 cells NVXaNoxVS3m2b4TvfIlkyqCjc4PhfS=> NEWyWII{KGSjeYO= NHX3ZWpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvOT2xN{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;NzFOwG0> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUe2Okc,OTh4MkW3OlY9N2F-
human HuH6 cells NU\UWpQ4S3m2b4TvfIlkyqCjc4PhfS=> M{XhUVMh\GG7cx?= MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg3KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGNEPTB;NzFOwG0> M1T4b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human Huh5-2 cells NX3nS5JKS3m2b4TvfIlkyqCjc4PhfS=> Mli3N{Bl[Xm| NH;zRY9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWh3LUKgZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KE2WVDDhd5NigSBuIFPDOVA:PiEQvF2= M{PDelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1O|Y3Lz5zOE[yOVc3PjxxYU6=
human HuH7 cells NYfWSldXS3m2b4TvfIlkyqCjc4PhfS=> M4DhR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDdiY3XscJMh[XO|ZYPz[YQh[XNicnXs[YF{\SCxZjDsZYN1[XSnIHTlbJllem:pZX7hd4UtKEmFNUC9OU45OSEQvF2= MlLRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7NEOzPVAoRjJyOUSzN|kxRC:jPh?=
human 2008 cells NYn2XYhTTnWwY4Tpc44h[XO|YYm= M{TsdGlvcGmkaYTpc44hd2ZiaIXtZY4hVVKSMTDpckBpfW2jbjCyNFA5KGOnbHzzMEBKSzVyPUSuO|gh|ryP M2DVOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{C3PFg1Lz5zOEewO|g5PDxxYU6=
human HeLa cells Mn3vSpVv[3Srb36gZZN{[Xl? NI\JdpJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJWFZvMUigbY5n\WO2ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEeg[IF6e+,:jDDUR|UxRTRwNjFOwG0> NGnRbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
leukemia CEM cells Ml\FSpVv[3Srb36gZZN{[Xl? M4\KWmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDNSHIyKFBvR3z5Z49xem:2ZXnuJGFDSyCWcnHud5BwenSncjD1d4lv\yCuZYXr[Y1q[SCFRV2gZ4VtdHNuIFnDOVA:Oy52IN88US=> NG[yVII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkO2NVM5Pyd-MUKzOlE{QDd:L3G+
human Caco2 cells NYr6fFZ4TnWwY4Tpc44h[XO|YYm= MYnubIljcXSrb36gc4YhWC2pcDDpckBpfW2jbjDDZYNwOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[Ilod3irbjDl[oZtfXhuIFnDOVA:Oi57IN88US=> NH;5fGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O4NlQ2QCd-MkOzPFI1PTh:L3G+
human MDA435/LCC6MDR cells NHi1e2lEgXSxdH;4bYPDqGG|c3H5 M3;sO|czcA>? NYnjVI1ZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiZYjwdoV{e2mwZzDNSHIh[W[2ZYKgO|IhcHK|78{MJGlEPTB;Mj64JO69VQ>? NUPjdlNKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNlA1ODJpPkKyN|IxPDB{PD;hQi=>
guinea pig ventricular myocytes NUDDO4ZkTnWwY4Tpc44h[XO|YYm= M4TEPWlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1? NEHIWYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2NVAxOCd-MkK3OlExODB:L3G+
KB/MDR cell NGO3elZHfW6ldHnvckBie3OjeR?= M2rLVVczKGh? NULIW2dZUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEuEL13EVkBk\WyuIHzpcoUh[W[2ZYKgO|IhcHJib3[g[JJ2\yCneIDvd5Vz\SxiSVO1NF0yNjVizszN NX7NSmlyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFE6QTFpPkG1OFgyQTlzPD;hQi=>
A2780/ADR cells NGHpZnVHfW6ldHnvckBie3OjeR?= NWTDW256UW6qaXLpeIlwdiCxZjDQMYdtgWOxcILveIVqdiCneIDy[ZN{\WRiaX6gRVI4QDBxQVTSJINmdGy|IHL5JINidGOnaX6gRW0h[XO|YYpvwKwhUUN3ME2xMlQyOjV2IN88US=> NVvk[olHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells MXLGeY5kfGmxbjDhd5NigQ>? NHjTPWsyKM7:TR?= NFfaN|g4OiCq NYDuR4RjWG:2ZX70bYF1cW:wIH;mJIRwgG:{dXLpZ4lvNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIwWjdiY3XscJMh[XO|ZYPz[YQh[XNiZH;4c5J2[mmlaX6gTWM2OCCjdDCxJJVOKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE44KM7:TR?= MmfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{SwOFEoRjJ3NkO0NFQyRC:jPh?=
LLC-PK1 epithelial cells NXzoPVVUTnWwY4Tpc44h[XO|YYm= MoPFTY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYjDlfJBz\XO|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG|c3H589yNKEmFNUC9NE44KM7:TR?= NX7LUZo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
mouse L5178 cells MUPGeY5kfGmxbjDhd5NigQ>? M{SwRVIxKG2rboO= NF7adYZKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1KxMY1m\GmjdHXkJJJpd2SjbXnu[UAyOjNiZX\mcJV5KGmwIH3veZNmKEx3MUe4JINmdGy|IHX4dJJme3OrbnegbJVu[W5iTVTSNUBi\nSncjCyNEBucW6|IHL5JGZCS1NiYX7hcJl{cXNuIFnDOVA:OC54NTFOwG0> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2MUe0OUc,OjF|NEG3OFU9N2F-
KB cell M17o[GZ2dmO2aX;uJIF{e2G7 MmLYTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDKGOnbHygcIlv\SCjZoTldkA4OiCqIH;mJIRzfWdiZYjwc5N2emVuIFnDOVA:OC54IN88US=> NYTXVmN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFE6QTFpPkG1OFgyQTlzPD;hQi=>
mouse mammary carcinoma cell line EMT6/AR 1.0 NHTobHhHfW6ldHnvckBie3OjeR?= MX;S[ZZmenOjbDDl[oZm[3Rib36geIhmKGGlY4XteYxifGmxbjDv[kBcO0ifLTDkZZVvd3K3YnnjbY4hcW5ibX;1d4UhdWGvbXHyfUBk[XKlaX7vcYEh[2WubDDsbY5mKEWPVE[vRXIhOS5yLDDJR|UxRTBwNESg{txO NG[0cpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEC5PFY4OSd-MUCwPVg3PzF:L3G+
CK1 cells NYjQ[IVnTnWwY4Tpc44h[XO|YYm= MXrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIVHYuOzNiaX7m[YN1\WRiaX6gR2syKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiNzDkZZl{NCCWQ{WwQVAvOyEQvF2= NIrmUoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKxNlExOCd-MUiyNVIyODB:L3G+
HEK293 cells NWPwb5BGTnWwY4Tpc44h[XO|YYm= M1v2[GlvcGmkaYTpc44hd2ZiT1HUVFFDOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNicILveIVqdi2vZXTpZZRm\CCyaYTheoF{fGG2aX6geZB1[Wun78{MJGtqRTBwMjFOwG0> NYrCTZZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFc6QDZpPkKyOVg4QTh4PD;hQi=>
human MDA435/LCC6MDR cells NUX0OYhzTnWwY4Tpc44h[XO|YYm= NXHkZ2ZrPSCmYYnz M1\2cm1w\HWuYYTpc44hd2ZiUD3ndEApfW6tbn;3ckBwemmpaX6pJJRz[W6|ZnXjeIVlKGmwIHj1cYFvKE2GQUSzOU9NS0N4TVTSJINmdGy|IHHzd4V{e2WmIHHzJJJmfmW{c3nu[{Bx[WOuaYThfIVtKHKnc3nzeIFv[2VibXXhd5Vz\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA2KGSjeYOgZpkhVVSVIHHzd4F6NCCHQ{WwQVAvODN{IN88UU4> NWPqVIRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFUyQTVpPkK1PVg2OTl3PD;hQi=>
human PBMC Mn:zVJJwdGmoZYLheIlwdiCjc4PhfS=> M2\vPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFLNR{whUUN3ME2xNkBvVQ>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ5OUOwPUc,OTV4N{mzNFk9N2F-
mouse T cells NF7rcoVHfW6ldHnvckBie3OjeR?= MnvwOEBl[Xm| MkDhTY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hdW:3c3WgWEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIT3c{B4[XlibXn4[YQhdHmvcHjvZ5l1\SC{ZXHjeIlwdiCjZoTldkA1KGSjeYOgZpkhVVSWIHHzd4F6NCCrY{WwQVEyKG6P Mnu4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OES5PFcoRjJ{OUi0PVg4RC:jPh?=
human splenocytes MkC5SpVv[3Srb36gZZN{[Xl? MVy0JIRigXN? MkOyTY1ufW6xc4XwdJJme3OrdnWgZYN1cX[rdImgbY4hcHWvYX6gd5Bt\W6xY4n0[ZMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifdHj5cYllcW6nIHnuZ49zeG:{YYTpc44h[W[2ZYKgOEBl[Xm|IHL5JIFtdG:pZX7pZ{BucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJIF{e2G7LDDJR|UxRTFyIH7N NU\HTlRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlc5OzFpPkG5PFI4QDNzPD;hQi=>
human T-cell MmL0SpVv[3Srb36gZZN{[Xl? M3;uVmlvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUMYNmdGxicILv[JVkfGmxbjDv[kBtgW2yaH;rbY5mKEmOLUKsJGlEPTB;OD6xJI5O M3fTdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd3M{ezN|EoRjd3M{ezN|E9N2F-
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MDA-435/LCC6 and MDA-435/LCC6-MDRI NH72[npHfW6ldHnvckBie3OjeR?= MX7Dc45k\W62cnH0bY9vKHSqYYSgdoVlfWOnczD0bIUh\GmoZnXy[Y5k\SCrbjDy[ZZmenOjbDDv[kBFV1hiYXPjeY12dGG2aX;uJIJmfHenZX6gUWRCNTR|NT;MR2M3KGGwZDDNSGEuPDN3L1zDR|YuVUSUSTDj[YxteyCkeTC1NEUvNCCHQ{WwJF0hOC53IN88UU4> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NEG0N{c,OTF5OESxOFM9N2F-
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MDA-435/LCC6 and MDA-435/LCC6-MDRI NInQeWlHfW6ldHnvckBie3OjeR?= MYPDc45k\W62cnH0bY9vKHSqYYSgdoVlfWOnczDkbYZn\XKnbnPlJIlvKHKndnXyd4FtKG:oIGuzTH1XSkxiYXPjeY12dGG2aX;uJIJmfHenZX6gUWRCNTR|NT;MR2M3KGGwZDDNSGEuPDN3L1zDR|YuVUSUSTDj[YxteyCkeTC1NEUtKEWFNUCgQUAxNjZizszNMi=> NEmyR|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUe4OFE1Oyd-MUG3PFQyPDN:L3G+
MDA435/LCC6 MDR1 NFvKUmpHfW6ldHnvckBie3OjeR?= NXfVWJc1emW4ZYLzZYwhd2ZiVnnuZoxie3SrbnWgZYNkfW23bHH0bY9vKCiYaX7icIF{fGmwZUqgNE4xODVidV2pJIlvKE2GQUSzOU9NS0N4IF3EVlEh[2WubIOsJGVEPTBiPTCwMlYh|ryPLh?= NWT3fGkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3PFQyPDNpPkGxO|g1OTR|PD;hQi=>
MDA-435/LCC6 (Pgp-negative) M2nqW2Z2dmO2aX;uJIF{e2G7 MniwSYZn\WO2IH;uJJJmfmW{c3HsJI9nKESRWDDhZ4N2dXWuYYTpc44hcW5iTVTBMVQ{PS:OQ1O2JEhR\3BvbnXnZZRqfmVrIHPlcIx{NixiRVO1NEA:KDBwNzFOwG0v MoraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5OESxOFMoRjFzN{i0NVQ{RC:jPh?=
MDA-435/LCC6 MVnHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MUHHdo94fGhiaX7obYJqfG:{eTDhZ5Rqfmm2eTDvckBOTEFvNEO1M2xESzZiKGDndE1xd3OrdHn2[UkhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsd{4tKEmFNUCgQUAyKM7:TT6= M{DBclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
MDA-435/LCC6-MDRI M4LncGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 M{KyWWdzd3e2aDDpcohq[mm2b4L5JIFkfGm4aYT5JI9vKE2GQT20N|UwVEOFNj3NSHJKKCiSZ4CtcoVo[XSrdnWpJIh2dWGwIHLy[YF{fCClYX7j[ZIh[2WubIOuMEBKSzVyIE2gNU4yKM7:TT6= M3\hU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{i0NVQ{Lz5zMUe4OFE1OzxxYU6=
BTI-TN5B1-4 NI\CXphHfW6ldHnvckBie3OjeR?= MVvBWHAhcHmmcn;sfZNqeyCrbjDt[Y1jemGwZTDmdoFkfGmxbjDmdo9uKEirZ3igSol3\SBqQmTJMXRPPUJzLUSpJINmdGy|LDDLcUA:KDBwMUeg{txONg>? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd6NU[4OEc,OTF5OEW2PFQ9N2F-
Jurkat MUfGeY5kfGmxbjDhd5NigQ>? MYfJcohq[mm2aX;uJI9nKHC{b3T1Z5Rqd25ib3[gTWwuOiC|ZXPy[ZRqd25iaX6gTpVzc2G2IHPlcIx{NCCLQ{WwJF0hOC5yMEeg{txONg>? NXXF[5NKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG4PFE6QDRpPkGxPFgyQTh2PD;hQi=>
Jurkat M3HNTmZ2dmO2aX;uJIF{e2G7 NEO4R5RKdmirYnn0bY9vKG:oIIDyc4R2[3Srb36gc4Yh[mW2YT3nZYxi[3Sxc3nkZZNmKEmOLUKgVmdCKGmwIFr1dotifCClZXzsd{whUUN3MDC9JFAvODB6IN88UU4> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTh6MUm4OEc,OTF6OEG5PFQ9N2F-
LLC-PK1 M{\NbGZ2dmO2aX;uJIF{e2G7 NHnIPYFqdmO{ZXHz[UBqdiCYaX7icIF{fGmwZTDpcpRz[WOnbHz1cIFzKGGlY4XteYxifGmxbjDpckBOTFJzLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUAyNjNizszNMi=> Mm\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7NkGxNVMoRjFzOU[xNVE{RC:jPh?=
LLC-PK1 M4rjZ2Z2dmO2aX;uJIF{e2G7 MVXpcoNz\WG|ZTDpckBE[WylZXnuMWFOKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKGmwIF3EVlEu\XiycnXzd4lv\yCOTFOtVGsyKGOnbHzzMEBMcSB;IESuOlYh|ryPLh?= M{\nV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[xNVE{Lz5zMUm2NVEyOzxxYU6=
MDCK NYnqO3poTnWwY4Tpc44h[XO|YYm= MmDubY5pcWKrdHnvckBw\iCGaXfvfIlvKHS{YX7z[ZBqfGinbHnhcEB1emGwc4DvdpQhMGKjc3HsJJRwKGGyaXPhcEkhcW5iTVTSNU1mgHC{ZYPzbY5oKE2GQ1ugZ4VtdHNuIFvpJF0hOi5zODFOwG0v NHjFVng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGzOFk1PSd-MUKxN|Q6PDV:L3G+
MDCK M4CwT2Z2dmO2aX;uJIF{e2G7 M2i4d4lvcGmkaYTpc44hd2ZiVnnuZoxie3SrbnWgeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDCoZoF{[WxidH:gZZBq[2GuKTDpckBOWlB{LXX4dJJme3OrbnegUWREUyClZXzsd{whU2liPTC4MlEyKM7:TT6= M3jpbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUO0PVQ3Lz5zMkGzOFk1PjxxYU6=
LLC-PK1 MWHGeY5kfGmxbjDhd5NigQ>? M4P5[Ylv[3KnYYPlJIlvKEOjbHPlbY4uSU1iaX70doFk\WyudXzhdkBi[2O3bYXsZZRqd25iaX6gcYRzOWJvZYjwdoV{e2mwZzDMUGMuWEtzIHPlcIx{NCCNaTC9JFMvPSEQvF2u NYLXWYhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN|UzPjdpPkGyNlM2OjZ5PD;hQi=>
LLC-PK1 NIrVSmdHfW6ldHnvckBie3OjeR?= NF;VXWJqdmO{ZXHz[UBqdiCFYXzj[YlvNUGPIHnueJJi[2WubIXsZZIh[WOldX31cIF1cW:wIHnuJI1lejGjLXX4dJJme3OrbnegUGxENVCNMTDj[YxteyxiS3mgQUA{NjZ3IN88UU4> NUnScGhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyN|UzPjdpPkGyNlM2OjZ5PD;hQi=>
HL-60 MVfGeY5kfGmxbjDhd5NigQ>? MkfSTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIH\vdo16dHCncITp[IUhemWlZYD0c5IhME[SUjmgc4YhcHWvYX6gcIV2c2WvaXGgTGwuPjBiY3XscJMtKEmFMkCgQUA{NjZizszNMi=> M3TBO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN|g5Lz5zMkO2NVM5QDxxYU6=
High Five cells MoLzSpVv[3Srb36gZZN{[Xl? Mk\NbY5pcWKrdHnvckBw\iCWYYXyc4Np\W6xZHXvfJlkcG:uYYTlJJVxfGGtZTjDd2EhOzC3TTygN|AhLSCxZjDjc451em:uKTDpckBu\W2kcnHu[UB3\XOrY3zld{BxemWyYYLl[EBnem:vIFjp[4ghTmm4ZTDj[YxteyCrbn\lZ5Rm\CC5aYToJJRp\SCDQlPCNVEh[mGldXzveolzfXNuIFvpJF0hQS53IN88UU4> NXnKdXE3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0NFQzOzlpPkGyOFA1OjN7PD;hQi=>
MDCKII M3zxZ2Z2dmO2aX;uJIF{e2G7 M{fBR4lvcGmkaYTpc44hd2ZiQ3XybZZie3SjdHnuJJVxfGGtZTDpckBQSVSSMj3lfJBz\XO|aX7nJG1FS0uLSTDj[YxteyxiS3mgQUAxNjJ|ODFOwG0v NYL4eGdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK1N|g5OTNpPkGyOVM5QDF|PD;hQi=>
Caco-2 NXK3WVdoTnWwY4Tpc44h[XO|YYm= NVTCeYJ2fHKjboPldIl1cGWuaXHsJJRz[W6|cH;yeEBw\iCmaXfvfIlvKCikYYPhcEB1dyCjcHnjZYwqKGmwIFPhZ48uOiClZXzsd{whU2liPTCwMlM2KM7:TT6= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZ|NkG1N{c,OTJ4M{[xOVM9N2F-
SK-E2 NGHiWmdHfW6ldHnvckBie3OjeR?= M3fBb4lv[3KnYYPlJIlvKGKxZHnwfUBqdnS{YXPlcIx2dGG{IHHjZ5VufWyjdHnvckApSm:maYD5PkAxNjJidV2pJIlvKFONLVWyJINmdGy|IDjlfJBz\XO|aX7nJGJUTVBrLDDJR|UxKD1iNz61JO69VS5? M3fnUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O|U6Lz5zMkezPVc2QTxxYU6=
SK-E2 MkK3SpVv[3Srb36gZZN{[Xl? NEDldFBqdmO{ZXHz[UBqdiCmaXj5[JJw\my3b4Lld4NmcW5iaX70doFk\WyudXzhdkBi[2O3bYXsZZRqd25iKHTpbJllem:obIXvdoV{[2WrbkqgNUB2VSliaX6gV2suTTJiY3XscJMhMGW6cILld5NqdmdiQmPFVEktKEmFNUCgQUA4NjhizszNMi=> NX7Kc2VuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3N|k4PTlpPkGyO|M6PzV7PD;hQi=>
HEK293 MUDGeY5kfGmxbjDhd5NigQ>? NWD0cGZ[cW6qaXLpeIlwdiCxZjDQbIFtdG:rZHnuJJVxfGGtZTCoVIhidGyxaXTpclohOSC3TTmgbY4hV0GWUD3DMYV5eHKnc4PpcochUEWNMkmzJINmdGy|LDDLbUA:KDBwMjFOwG0v NIDsRWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWzNFkxPyd-MUS1N|A6ODd:L3G+
T-cells MWPGeY5kfGmxbjDhd5NigQ>? MX;Jcohq[mm2aX;uJI9nKGGubH;n[Y5q[yClZXzsd{1{fGmvdXzheIVlKHC{b3zp[oVz[XSrb36gbY4hdW:3c3WgWEBk\WyuczDifUBucXinZDDsfY1xcG:leYTlJJJm[WO2aX;uJI1mfGixZDygTWM2OCB;IECuNFMzKM7:TT6= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV7M{G4Nkc,OTR3OUOxPFI9N2F-
T-cells MUDGeY5kfGmxbjDhd5NigQ>? M{DU[GlvcGmkaYTpc44hd2ZiYXzsc4dmdmmlIHPlcIx{NXO2aX31cIF1\WRicILvcIln\XKjdHnvckBqdiCqdX3hckBVKGOnbHzzJIJ6KG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44hdWW2aH;kMEBKSzVyIE2gNE4xPzRizszNMi=> NGPl[Hc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEW5N|E5Oid-MUS1PVMyQDJ:L3G+
PMBC MYPGeY5kfGmxbjDhd5NigQ>? NXfu[5VbPzJiaILz MmLkSYZn\WO2IH;uJJBzd2yrZnXyZZRqd25ib3[gbJVu[W5icHXybZBp\XKjbDDicI9w\CCvb37vcpVkdGWjcjDj[YxteyBqUF3CR{kh[XRiN{KgbJIhfXOrbnegUXRVKGG|c3H5MEBKSzVyIE2gPE46PyEQvF2u M2\HfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MkCzNVY{Lz5zNUKwN|E3OzxxYU6=
mouse spleen cells MU\DfZRwfG:6aXPpeJkh[XO|YYm= NUfnbo9pS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJJNxdGWnbjDj[YxteyxiQ1O1NEA:KDFwM{Wg{txONg>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB6MUe2NUc,OTdyOEG3OlE9N2F-
MDCK2 NFnhOGJHfW6ldHnvckBie3OjeR?= MYnJcohq[mm2aX;uJI9nKGi3bXHuJG1FWjFvZHXw[Y5l\W62IHHjZ5VufWyjdHnvckBw\iClYXzj[YlvNUGPIHX4dJJme3OnZDDpckBOTEONMjDj[YxteyxiSVO1NEA:KDlwM{Sg{txONg>? M2PvSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[0N|I4Lz5zN{[2OFMzPzxxYU6=
HEK293 NGnUNmdHfW6ldHnvckBie3OjeR?= NUXoV3J{UW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVEhVDZ3Uj;UUVYhVDN|OVOvWG04KEZ5MkigcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJFUxLSCrbnjpZol1cW:wIH;mJINzd3O|IHzpcotqdmdiYomgbY1ufW6xYnzveEwhSWO2aY\peJkhRSByLkWg{txONg>? NUDxbIRHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ|PD;hQi=>
HEK293 MnLpSpVv[3Srb36gZZN{[Xl? MWfJcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPNjDMN|M6Sy:WTUegSlczQENibYX0ZY51KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIEWwKUBqdmirYnn0bY9vKG:oIHPyc5N{KGyrbnvpcoch[nliaX3teY5w[myxdDygRYN1cX[rdImgQUAxNjZizszNMi=> NYn0Ro9jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OFg2PjNpPkG3PFQ5PTZ|PD;hQi=>
HEK293 NEj0bY9HfW6ldHnvckBie3OjeR?= NYDaTXB3UW6qaXLpeIlwdiCxZjDjdo9{ey2uaX7rbY5oKG:oIHj1cYFvKFCpcDDUUVNVOTl7Uj;UUVYhVDN|OVOvWG04KEZ5MkjDJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{A2OCViaX7obYJqfGmxbjDv[kBkem:|czDsbY5scW6pIHL5JIludXWwb3Lsc5QtKEGldHn2bZR6KD1iMT6xJO69VS5? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh2OEW2N{c,OTd6NEi1OlM9N2F-
HEK293 Ml7XSpVv[3Srb36gZZN{[Xl? MVHJcohq[mm2aX;uJI9nKGO{b4PzMYxqdmurbnegc4YhcHWvYX6gVIdxKFSPNjDGN|Q{Wi:WTU[gUFM{QUNxVF23JGY4OjiFIH31eIFvfCCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHTyeYchdGW4ZXygZ4F2e2mwZzC1NEUhcW6qaXLpeIlwdiCxZjDjdo9{eyCuaX7rbY5oKGK7IHntcZVvd2Kub4SsJGFkfGm4aYT5JF0hOTFizszNMi=> NGLFRWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i0PFU3Oyd-MUe4OFg2PjN:L3G+
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Assay
Methods Test Index PMID
Western blot Phospho-AKT1 / AKT1 / PTEN / Phospho-BAD / BAD / Procaspase-3 Cyp-D / AIF / β-actin IL-8 / MCP-1 Actin / Smac β-actin / FIPV-N 12644839 29328412 9602633 12427831 22546085
Growth inhibition assay NPC death 20181586
IHC Th22 / Th17 / Th1 / M1 / Th2 Tregs / Bregs / pDCs / M2 Ki 67 / Fas-FasL IFN-γ / TNF-α WT-PCLS / FXRKO-PCLS 30916387 10931158 26482353
Immunofluorescence Human gingival fibroblast cells Human gingival fibroblast cells Calcein Rhod-Dex / Calcein cadherin-9 / vimentin 27857765 23593196 20584761 17668045
体内研究 Cyclosporin A是一种免疫抑制剂,小鼠,大鼠,和荷兰猪经非肠道给药或者口服给药都有效[6],临床上用于器官移植中预防免疫排斥。[7]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[3]
  • 磷酸酶试验:

    纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶,100 nM 钙调蛋白,以及5 μM 32P-标记磷酸化多肽,60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8),100 mM NaCI,6 mM MgCl2,0.5 mM 二硫苏糖醇, 每毫升0.1毫克牛血清白蛋白,以及0.1 mM CaCl2或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物, 5 μM 32P-标记的磷酸肽,和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸,一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca2+-不依赖性磷酸酶,而更高浓度时也会部分抑制Ca2+-依赖性的活性。30°C下培育15分钟,加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐,用闪烁计数测定数量。

细胞实验:[3]
  • Cell lines: Jurkat细胞(无性繁殖J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1小时
  • Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(106)悬浮于微量离心管1ml完全培养基中;1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中,细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次,在50μl低渗缓冲液中裂解,缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯,50 微克胰蛋白酶抑制剂,5微克亮抑酶肽,和5 微克抑肽酶。裂解液用液氮冷冻,再在30°C下融化,循环3次,随后在 4°C下以12,000×g离心10分钟。
动物实验:[6]
  • Animal Models: 大鼠
  • Dosages: 45毫克/千克
  • Administration: 口服

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O

5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 1202.61
化学式

C62H111N11O12

CAS号 59865-13-3
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04989686 Withdrawn Other: Microsampling Immunosuppression Children''s Hospital of Philadelphia March 2022 --
NCT05056779 Withdrawn Drug: Nemolizumab|Drug: CD14152 placebo Moderate-to-severe Atopic Dermatitis Galderma R&D January 2022 Phase 3
NCT05202145 Completed Drug: ALXN2050|Drug: Cyclosporine|Drug: Tacrolimus|Drug: MMF Healthy Alexion Pharmaceuticals January 11 2022 Phase 1
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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常见问题及建议解决方法

问题 1:
What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

回答:
Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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