Cyclosporin A

For research use only. Not for use in humans.

目录号:S2286 别名: Cyclosporine A 中文名称:环孢菌素A

Cyclosporin A  Chemical Structure

CAS No. 59865-13-3

Cyclosporin A (Cyclosporine A) 是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1073.15 现货
RMB 786.07 现货
RMB 1957.41 现货
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客户使用Selleck生产的Cyclosporin A 发表文献40篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Cyclosporin A (Cyclosporine A) 是一种免疫抑制剂,与亲环素结合,随后抑制calcineurin,无细胞试验中IC50为7 nM,广泛用于预防器官移植反应中的排斥反应。
靶点
calcineurin [1]
(Cell-free assay)
7 nM
体外研究

在T细胞中,Cyclosporin A与亲环蛋白(免疫亲和素)结合[1],形成亲环蛋白-Cyclosporin A复合体,其结合并抑制钙调磷酸酶。[2]Cyclosporin A 抑制钙调磷酸酶,IC50 是7 nM[3],随后阻断NF-AT核转运。 [4]Cyclosporin A通过抑制MTP(线粒体通透性转换孔)的开放而影响线粒体,IC50 是39 nM。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
rat H42E cells M{DCWWN6fG:2b4jpZ:Kh[XO|YYm= MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiSESySUBk\WyuczDhd5Nme3OnZDDhd{BqdnS{YXPlcIx2dGG{IFHUVEBt\X[nbDygWGM2OD12MjFOwG0> NH\IcHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWwNlA2QCd-MUm1NFIxPTh:L3G+
mouse L929 cells MYPDfZRwfG:6aXRCpIF{e2G7 NV33SJhPOyCmYYnz MoS5R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUFkzQSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRUKGG|c3H5MEBKSzVyPUOwJO69VQ>? M4XobFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human Huh-Mono cells NEDhXGREgXSxdH;4bYPDqGG|c3H5 M2rwWFMh\GG7cx?= MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeYguVW:wbzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F697zOIFPDOVA:OjRizszN NWPJR49YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU4PjZpPkG4OlI2PzZ4PD;hQi=>
MDCK cells M1LKd2N6fG:2b4jpZ:Kh[XO|YYm= NEnKUHJEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFPLJINmdGy|LDDUR|UxRTlwOTFOwG0> MmTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
human LCC-6 cells M{C2TGN6fG:2b4jpZ:Kh[XO|YYm= NH\CZXk4OiCq MmG4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUGNENTZiY3XscJMh[W[2ZYKgO|IhcHK|LDDJR|UxRThwMzFOwG0> Ml;yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|MkC0NFIoRjJ{M{KwOFAzRC:jPh?=
human HeLa cells M4PMfGN6fG:2b4jpZ:Kh[XO|YYm= M33UTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMtKFSFNUC9PE4{KM7:TR?= M4fKRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkGyNVAxLz5zOEKxNlExODxxYU6=
african green monkey Vero cells M1rTSWZ2dmO2aX;uJIF{e2G7 NIj0cmEzPCCq MVPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDX[ZN1KE6rbHWgeolzfXNiaX7m[YN1\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhXmW{bzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckB3cXKjbDDSUmEhe3mwdHjld4l{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:QCEQvF2= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUK4Okc,OTl2NUGyPFY9N2F-
human Huh-9-13 cells MmfvR5l1d3SxeHnjxsBie3OjeR?= NH:2WWc{KGSjeYO= NHPiXYFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfWhvOT2xN{Bk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G778{MJGNEPTB;NzFOwG0> NHTKOoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human HuH6 cells M4HBNWN6fG:2b4jpZ:Kh[XO|YYm= MoD0N{Bl[Xm| NGnk[HJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh4IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEOFNUC9O{DPxE1? NHfQO|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human Huh5-2 cells NXvTdFRFS3m2b4TvfIlkyqCjc4PhfS=> NGCxPXY{KGSjeYO= M1fZfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2cDVvMjDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6KCxiQ1O1NF03KM7:TR?= NIHYSVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVc3Pid-MUi2NlU4PjZ:L3G+
human HuH7 cells NWPzWoM{S3m2b4TvfIlkyqCjc4PhfS=> MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KHKnbHXhd4Uhd2ZibHHjeIF1\SCmZXj5[JJw\2WwYYPlMEBKSzVyPUWuPFEh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl2M{O5NEc,OjB7NEOzPVA9N2F-
human 2008 cells NGe0UXRHfW6ldHnvckBie3OjeR?= MXTJcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD12Lke4JO69VQ>? NFHZbVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEewO|g5PCd-MUi3NFc5QDR:L3G+
human HeLa cells M{HBVGZ2dmO2aX;uJIF{e2G7 NVPHPGc1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUFCYLUG4JIlv\mWldHXkJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXKjbDDy[ZBtcWOjdHnvckBi\nSncjC3JIRigXQxvJygWGM2OD12Lk[g{txO MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{MUKxNFAoRjF6MkGyNVAxRC:jPh?=
leukemia CEM cells NEDPd4FHfW6ldHnvckBie3OjeR?= NHrKc2VKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUHWvYX6gUWRTOSCSLVfsfYNweHKxdHXpckBCSkNiVILhcpNxd3K2ZYKgeZNqdmdibHX1b4VucWFiQ1XNJINmdGy|LDDJR|UxRTNwNDFOwG0> M2WxPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{M{[xN|g4Lz5zMkO2NVM5PzxxYU6=
human Caco2 cells M1jDcWZ2dmO2aX;uJIF{e2G7 NWLIT|J4dmirYnn0bY9vKG:oIGCt[5AhcW5iaIXtZY4hS2Glb{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGSrZ3;4bY4h\W[obIX4MEBKSzVyPUKuPUDPxE1? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN6MkS1PEc,OjN|OEK0OVg9N2F-
human MDA435/LCC6MDR cells NHvxNYlEgXSxdH;4bYPDqGG|c3H5 NWmxWJMzPzKq MlnMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCPDN3L1zDR|ZOTFJiY3XscJMh\XiycnXzd4lv\yCPRGKgZYZ1\XJiN{KgbJJ{97zOIFnDOVA:Oi56IN88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MESwNkc,OjJ|MkC0NFI9N2F-
guinea pig ventricular myocytes Mln3SpVv[3Srb36gZZN{[Xl? M4fVPGlvcGmkaYTpc44hd2ZiTD30fZBmKGOjbHPpeY0h[2ijbn7lcEBu\WG|dYLl[EB2e2mwZzD3bI9t\S2lZXzsJJBifGOqIHPsZY1xKGmwIHf1bY5m[SCyaXegeoVvfHKrY4XsZZIhdXmxY4n0[ZMtKEmFNUC9NkDPxE1? NEjNWJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2NVAxOCd-MkK3OlExODB:L3G+
KB/MDR cell MVLGeY5kfGmxbjDhd5NigQ>? MV63NkBp MVjJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgT2IwVUSUIHPlcIwhdGmwZTDh[pRmeiB5MjDodkBw\iCmcoXnJIV5eG:|dYLlMEBKSzVyPUGuOUDPxE1? M3SxR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEixPVkyLz5zNUS4NVk6OTxxYU6=
A2780/ADR cells Mn;BSpVv[3Srb36gZZN{[Xl? Mn7ETY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckBmgHC{ZYPz[YQhcW5iQUK3PFAwSUSUIHPlcIx{KGK7IHPhcINmcW5iQV2gZZN{[XoxvJygTWM2OD1zLkSxNlU1KM7:TR?= NWX3SWhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQTRpPkG3PFkxODl2PD;hQi=>
human K562/R7 cells NV7K[5RlTnWwY4Tpc44h[XO|YYm= M3;sSVEh|ryP NVK1b3NJPzJiaB?= MonjVI91\W62aXH0bY9vKG:oIHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGGpYXnud5Qh\G:6b4L1ZolkcW5vcnXzbZN1[W62IHj1cYFvKEt3NkKvVlch[2WubIOgZZN{\XO|ZXSgZZMh\G:6b4L1ZolkcW5iSVO1NEBifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD63JO69VQ>? NF;XWVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[zOFA1OSd-MkW2N|QxPDF:L3G+
LLC-PK1 epithelial cells MWnGeY5kfGmxbjDhd5NigQ>? MomwTY5pcWKrdHnvckBw\iCSLXfsfYNweHKxdHXpckwhdW:3c3WgUE1u\HJzYjDlfJBz\XO|ZXSgbY4hVEyFLWDLNUBmeGm2aHXsbYFtKGOnbHzzJJV{cW6pIHPhcINmcW5vQV2gdI9t[XKrc3H0bY9vKGG|c3H589yNKEmFNUC9NE44KM7:TR?= NWXmfJBGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2PVk{QDlpPkGyOlk6Ozh7PD;hQi=>
mouse L5178 cells NGTJblVHfW6ldHnvckBie3OjeR?= NHW0eI0zOCCvaX7z NE\jUoxKdmirYnn0bY9vKG:oIHj1cYFvKEGEQ1KxMY1m\GmjdHXkJJJpd2SjbXnu[UAyOjNiZX\mcJV5KGmwIH3veZNmKEx3MUe4JINmdGy|IHX4dJJme3OrbnegbJVu[W5iTVTSNUBi\nSncjCyNEBucW6|IHL5JGZCS1NiYX7hcJl{cXNuIFnDOVA:OC54NTFOwG0> NVTSfm9zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFE4PDVpPkKxN|QyPzR3PD;hQi=>
KB cell Ml\tSpVv[3Srb36gZZN{[Xl? Mnm0TY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGtDKGOnbHygcIlv\SCjZoTldkA4OiCqIH;mJIRzfWdiZYjwc5N2emVuIFnDOVA:OC54IN88US=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MUm5NUc,OTV2OEG5PVE9N2F-
mouse mammary carcinoma cell line EMT6/AR 1.0 M4q2fGZ2dmO2aX;uJIF{e2G7 NH2xZmNT\X[ncoPhcEBm\m[nY4Sgc44hfGinIHHjZ5VufWyjdHnvckBw\iCdM1jdMUBl[XWwb4L1ZolkcW5iaX6gcY92e2VibXHtcYFzgSClYYLjbY5wdWFiY3XscEBtcW6nIFXNWFYwSVJiMT6wMEBKSzVyPUCuOFQh|ryP NXvnVXBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwPVg3PzFpPkGwNFk5PjdzPD;hQi=>
CK1 cells MluwSpVv[3Srb36gZZN{[Xl? Ml\3RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTHBXNTN|IHnu[oVkfGWmIHnuJGNMOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEeg[IF6eyxiVFO1NF0xNjNizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJzMkGwNEc,OTh{MUKxNFA9N2F-
HEK293 cells M3vjXGZ2dmO2aX;uJIF{e2G7 MXfJcohq[mm2aX;uJI9nKE:DVGCxRlEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KHC{b4TlbY4udWWmaXH0[YQheGm2YY\hd5RifGmwIIXweIFs\e,:jDDLbV0xNjJizszN NV;FfXNWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFc6QDZpPkKyOVg4QTh4PD;hQi=>
human MDA435/LCC6MDR cells M1XON2Z2dmO2aX;uJIF{e2G7 NFXqUoo2KGSjeYO= NG\XW2FOd2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpcocheGGlbHn0ZZhmdCC{ZYPpd5RidmOnIH3lZZN2emWmIHHzJINmdGxic4Xyeol3[WxiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5NigSxiRVO1NF0xNjB|MjFOwG0v M1vnNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUi1NVk2Lz5{NUm4OVE6PTxxYU6=
human PBMC NIXQXoZRem:uaX\ldoF1cW:wIHHzd4F6 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCETVOsJGlEPTB;MUKgcm0> NHi0fGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[3PVMxQSd-MUW2O|k{ODl:L3G+
mouse T cells M3zCR2Z2dmO2aX;uJIF{e2G7 NG\hRWE1KGSjeYO= NWrkZmtlUW2vdX7vd5VxeHKnc4PpeoUh[WO2aY\peJkhcW5ibX;1d4UhXCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJR4dyC5YYmgcYl5\WRibIntdIhw[3m2ZTDy[YFkfGmxbjDh[pRmeiB2IHThfZMh[nliTWTUJIF{e2G7LDDpZ|UxRTFzIH7N MnPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OES5PFcoRjJ{OUi0PVg4RC:jPh?=
human splenocytes MkPhSpVv[3Srb36gZZN{[Xl? MmjUOEBl[Xm| NEDtVlVKdW23bn;zeZBxemW|c3n2[UBi[3Srdnn0fUBqdiCqdX3hckB{eGynbn;jfZRmeyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFt|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCjZoTldkA1KGSjeYOgZpkh[Wyub3flcolkKG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44h[XO|YYmsJGlEPTB;MUCgcm0> Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mke4N|EoRjF7OEK3PFMyRC:jPh?=
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human Jurkat cells NVf0cYdQTnWwY4Tpc44h[XO|YYm= MUKxJIg> MnyxRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDKeZJs[XRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidnSrLVPEN{9ETDJ6IHHueIljd2S7LXnu[JVk\WRiSVytNkBxem:mdXP0bY9vKHS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJIFvfGlvQ1SzM2NFOjhiYX70bYJw\HliY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJIJ6KGmvbYXuc5N2eHC{ZYPzbY9vKGG|c3H5MEBGSzVyPUWgcm0> M4LiO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOzO|k2Lz5zOUmzN|c6PTxxYU6=
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HL-60 Mn7NSpVv[3Srb36gZZN{[Xl? MkjITY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIH\vdo16dHCncITp[IUhemWlZYD0c5IhME[SUjmgc4YhcHWvYX6gcIV2c2WvaXGgTGwuPjBiY3XscJMtKEmFMkCgQUA{NjZizszNMi=> MkLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ|NkGzPFgoRjF{M{[xN|g5RC:jPh?=
High Five cells M2HUVWZ2dmO2aX;uJIF{e2G7 NIfr[ldqdmirYnn0bY9vKG:oIGTheZJw[2inbn;k[Y95gWOqb3zheIUhfXC2YXvlLGN{SSB|MIXNMEA{OCBnIH;mJINwdnS{b3ypJIlvKG2nbXLyZY5mKH[nc3njcIV{KHC{ZYDhdoVlKG[{b32gTIlocCCIaY\lJINmdGy|IHnu[oVkfGWmIIfpeIghfGinIFHCR2IyOSCkYXP1cI93cXK3czygT4khRSB7LkWg{txONg>? Ml7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MESyN|koRjF{NEC0NlM6RC:jPh?=
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HEK293 M3zHTGZ2dmO2aX;uJIF{e2G7 NFywbnBqdmirYnn0bY9vKG:oIGDoZYxtd2mmaX6geZB1[WunIDjQbIFtdG:rZHnuPkAyKHWPKTDpckBQSVSSLVOt[ZhxemW|c3nu[{BJTUt{OUOgZ4VtdHNuIFvpJF0hOC5{IN88UU4> MlrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3M{C5NFcoRjF2NUOwPVA4RC:jPh?=
T-cells MmPqSpVv[3Srb36gZZN{[Xl? M1mwVGlvcGmkaYTpc44hd2ZiYXzsc4dmdmmlIHPlcIx{NXO2aX31cIF1\WRicILvcIln\XKjdHnvckBqdiCvb4Xz[UBVKGOnbHzzJIJ6KG2reHXkJIx6dXCqb3P5eIUhemWjY4Tpc44hdWW2aH;kMEBKSzVyIE2gNE4xOzJizszNMi=> NVT5bZExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1PVMyQDJpPkG0OVk{OTh{PD;hQi=>
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mouse spleen cells MmnOR5l1d3SxeHnjbZR6KGG|c3H5 NYXBRpFSS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJJNxdGWnbjDj[YxteyxiQ1O1NEA:KDFwM{Wg{txONg>? NYTRbVJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewPFE4PjFpPkG3NFgyPzZzPD;hQi=>
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... Click to View More Cell Line Experimental Data

体内研究 Cyclosporin A是一种免疫抑制剂,小鼠,大鼠,和荷兰猪经非肠道给药或者口服给药都有效[6],临床上用于器官移植中预防免疫排斥。[7]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[3]
- 合并

磷酸酶试验:

纯化的牛脑钙调磷酸酶和钙调蛋白是购买的。含纯化酶的反应混合物包含100 nM 钙调磷酸酶,100 nM 钙调蛋白,以及5 μM 32P-标记磷酸化多肽,60 μl (总体积) 试验缓冲液包含20 mM Tris (pH 8),100 mM NaCI,6 mM MgCl2,0.5 mM 二硫苏糖醇, 每毫升0.1毫克牛血清白蛋白,以及0.1 mM CaCl2或5 mM EGTA。含细胞裂解物的反应混合物包含20 μl of未稀释裂解物, 5 μM 32P-标记的磷酸肽,和40 μl 试验缓冲液。反应混合物包含50 μM 多肽412或413和/或 500 nM冈田酸,一种磷酸酶1和2A的特异性抑制剂; 500 nM 冈田酸足以抑制Ca2+-不依赖性磷酸酶,而更高浓度时也会部分抑制Ca2+-依赖性的活性。30°C下培育15分钟,加入0.5 ml包含5% 三氯乙酸的100 mM 磷酸钾缓冲液(pH 7.0)终止反应。Dowex阳离子交换色谱分离无机磷酸盐,用闪烁计数测定数量。
细胞实验:[3]
- 合并
  • Cell lines: Jurkat细胞(无性繁殖J77)
  • Concentrations: ~100 nM
  • Incubation Time: 1小时
  • Method: 免疫抑制剂以细胞治疗浓度的1000倍溶于乙醇。细胞(106)悬浮于微量离心管1ml完全培养基中;1 μl乙醇或者Cyclosporin A乙醇溶液加入反应液中,细胞在37°C下培育1小时。细胞在冰浴下用1ml PBS洗两次,在50μl低渗缓冲液中裂解,缓冲液每毫升包含50 mM Tris (pH 7.5); 0.1 mM EGTA; 1 mM EDTA; 0.5 mM二硫苏糖醇; 以及50 微克苯甲基磺酰氯,50 微克胰蛋白酶抑制剂,5微克亮抑酶肽,和5 微克抑肽酶。裂解液用液氮冷冻,再在30°C下融化,循环3次,随后在 4°C下以12,000×g离心10分钟。
    (Only for Reference)
动物实验:[6]
- 合并
  • Animal Models: 大鼠
  • Dosages: 45毫克/千克
  • Administration: 口服
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL warmed (83.15 mM)
Ethanol 100 mg/mL warmed (83.15 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 1202.61
化学式

C62H111N11O12

CAS号 59865-13-3
储存条件 粉状
溶于溶剂
别名 Cyclosporine A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04023760 Completed Drug: Apixaban Pharmacokinetics|Kidney Transplant|Lung Transplant University of Saskatchewan|Saskatchewan Health Research Foundation|Lung Association of Saskatchewan June 26 2019 Phase 4
NCT03865888 Completed Drug: Cyclosporins|Drug: Tacrolimus Dry Eye|Sjogren Syndrome Pavly Moawd|Cairo University October 30 2018 Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    What is the difference between S2286 (cyclosporin A) and S1514 (cyclosporin)?

  • 回答:

    Cyclosporine is a mixture of Cyclosporine A, derivatives of Cyclosporine A, salts of Cyclosporine A. Cyclosporine A is an especially useful Cyclosporine component.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID