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Geldanamycin

别名: NSC 122750 中文名称：格尔德霉素

Geldanamycin是一种天然的HSP90抑制剂，Kd为1.2 μM，特异性干扰糖皮质激素受体(GR)/HSP联合。Geldanamycin 通过减少宿主的炎症反应来减轻病毒感染引起的ALI(急性肺损伤)/ARDS(急性呼吸窘迫综合征)。

Geldanamycin Chemical Structure

Geldanamycin Chemical Structure

CAS: 30562-34-6

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 3131.63 现货
5mg RMB 1407.44 现货
50mg RMB 5493.81 现货
1g RMB 32678.1 现货
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批次： 纯度： 99.84%
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Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
rat neuronal cells Cytotoxicity assay 10 uM 24 hrs Cytotoxicity against rat neuronal cells at 10 uM after 24 hrs 17276679
PP30 Function assay 100 uM 3 hrs Inhibition of human HSP90alpha expressed in yeast PP30 cells co-expressing HSF-lacZ reporter assessed as induction of heat shock response at 100 uM after 3 hrs 21129982
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Her2 degradation at 500 nM after 24 hrs by Western blot method 21861487
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Raf degradation at 500 nM after 24 hrs by Western blot method 21861487
MCF7 Function assay 500 nM 24 hrs Inhibition of Hsp90 in human MCF7 cells assessed as induction of Akt degradation at 500 nM after 24 hrs by Western blot method 21861487
SKBR3 Function assay 0.5 uM 18 hrs Inhibition of HSP90 in human SKBR3 cells assessed as aggregation of intracellular HER2 at 0.5 uM after 18 hrs by DAPI staining-based immunofluorescence microscopic analysis 23859777
CL1-5 Function assay 2 uM 16 hrs Inhibition of HSP90 in human CL1-5 cells assessed as reduction of total Akt at 2 uM after 16 hrs by Western blotting analysis 24428777
MDA-MB 231 Function assay 0.5 uM 24 hrs Inhibition Hsp90 in human MDA-MB 231 cells assessed as increase in HSP70 protein level at 0.5 uM incubated for 24 hrs by Western blot method 25277067
PC12 Function assay 10 uM 3 to 16 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of intracellular D-aspartate content at 10 uM measured at 3 to 16 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis relative to vehicle-t 26642769
PC12 Function assay 10 uM 6 to 16 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of total D-aspartate content at 10 uM measured at 6 to 16 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis relative to vehicle-treated c 26642769
PC12 Function assay up to 10 uM 24 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of D-aspartate level in cell culture medium up to 10 uM after 24 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis 26642769
A549 Function assay 5 to 10 uM 24 hrs Inhibition of HSP90alpha in human A549 cells assessed as upregulation of HSP90alpha expression at 5 to 10 uM after 24 hrs by Western blot analysis 29486954
A549 Function assay 5 to 10 uM 24 hrs Inhibition of HSP90beta in human A549 cells assessed as upregulation of HSP90beta expression at 5 to 10 uM after 24 hrs by Western blot analysis 29486954
Jurkat Function assay 1 uM Inhibition of to HSP90 in FADD deficient human Jurkat cells assessed as RIP protein degradation at 1 uM by Western blot 18408713
SK-BR-3 Function assay 50 mg/kg Inhibition of oncogene product p185 erbB-2 from human breast cancer cells(SK-BR-3 cells) at 50 mg/kg dose, IC50=0.07μM 7562911
SH-SY5Y Neuroprotection assay 10 nM Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in retinoic acid-differentiated human SH-SY5Y cells assessed as lactate dehydrogenase release at 10 nM 19138859
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Akt degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Raf degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Hsp90 degradation at 0.5 uM by Western blot analysis 21273068
MCF7 Function assay 0.5 uM Inhibition of Hsp90 in human MCF7 cells assessed as Her2 degradation at 0.5 uM by Western blot analysis 21273068
MDA-MB 231 Function assay 0.5 and 5 uM Inhibition of HSP90 in human MDA-MB 231 cells assessed increase in HSP70 protein levels at 0.5 and 5 uM by Western blot method 25105924
KPL4 Function assay 40 hrs Inhibition of Hsp90 in human KPL4 cells assessed as depletion of Raf-1 level after 40 hrs, MEC=0.33μM 18243703
KPL4 Function assay 40 hrs Inhibition of Hsp90 in human KPL4 cells assessed as depletion of ErbB2 level after 40 hrs, MEC=0.11μM 18243703
P19 Cytotoxicity assay 18 hrs Cytotoxicity against mouse P19 cells after 18 hrs, IC50=0.1μM 17442565
SKBR3 Function assay 24 hrs Inhibition of Hsp90-mediated HER2 degradation in human SKBR3 cells after 24 hrs by Western blot 18816111
SKBR3 Function assay 24 hrs Inhibition of Hsp90-mediated Raf degradation in human SKBR3 cells after 24 hrs by Western blot 18816111
HuH7 Antiviral assay 3 days Antiviral activity against Hepatitis C virus genotype 1b Con1 infected in human HuH7 cells assessed as GAPDH RNA or 18S rRNA level after 3 days by qRT-PCR analysis, EC50=0.00025μM 18936191
HuH7 Antiviral assay 3 days Antiviral activity against Hepatitis C virus genotype 1b Con1 infected in human HuH7 cells assessed as GAPDH RNA or 18S rRNA level after 3 days selected with 40 nM HCV-796 and 800 nM boceprevir by qRT-PCR analysis, EC50=0.0012μM 18936191
SKBR3 Cytotoxicity assay 72 hrs Cytotoxicity against human SKBR3 cells after 72 hrs by celltiter-glo assay, IC50=0.041μM 19405528
A431 Antiproliferative assay 72 hrs Antiproliferative activity against human A431 cells after 72 hrs, IC50=0.2μM 20655237
HCT116 Antiproliferative assay 4 days Antiproliferative activity against human HCT116 cells measured on day 4 by Cell titer-glo assay, EC50=0.03μM 21420297
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells at 72 hrs by MTS assay, IC50=0.053μM 22162786
SKBR3 Antiproliferative assay 72 hrs Antiproliferative activity against estrogen receptor deficient human SKBR3 cells after 72 hrs, IC50=0.0085μM 23648180
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells expressing estrogen receptor after 72 hrs, IC50=0.0098μM 23648180
A549 Cytotoxicity assay 2 days Cytotoxicity against human A549 cells after 2 days by AlamarBlue assay, IC50=0.15μM 23947794
L6 Cytotoxicity assay 72 hrs Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay, IC50=6μM 24580531
Sf9 Function assay 16 hrs Displacement of GM-BODIPY from human full length HSP90 alpha expressed in baculovirus-infected Sf9 cells after 16 hrs by fluorescence polarization assay, IC50=0.074μM 24751441
MDA-kb2 Function assay 18 hrs Inhibition of HSP90 in human MDA-kb2 cells assessed as reduction in glucocorticoid receptor-dependent luciferase expression after 18 hrs by firefly luciferase reporter gene assay, IC50<0.01μM 24984936
MCF7 Antiproliferative assay 72 hrs Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay, IC50=0.04μM 25075762
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=0.05μM 25105924
LNCAP Cytotoxicity assay 72 hrs Cytotoxicity against human LNCAP cells after 72 hrs by MTT assay, IC50=0.43μM 25105924
HUVEC Cytotoxicity assay 72 hrs Cytotoxicity against HUVEC cells after 72 hrs by MTT assay, IC50=0.019μM 25277067
A431 Cytotoxicity assay 72 hrs Cytotoxicity against human A431 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
BGC823 Cytotoxicity assay 72 hrs Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50=0.04μM 25277067
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=0.05μM 25277067
A549 Cytotoxicity assay 72 hrs Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=0.097μM 25277067
HL7702 Cytotoxicity assay 72 hrs Cytotoxicity against human HL7702 cells after 72 hrs by MTT assay, IC50=0.141μM 25277067
SW480 Cytotoxicity assay 72 hrs Cytotoxicity against human SW480 cells after 72 hrs by MTT assay, IC50=0.31μM 25277067
HeLa Cytotoxicity assay 72 hrs Cytotoxicity against human HeLa cells after 72 hrs by MTT assay, IC50=0.798μM 25277067
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay, IC50=0.0427μM 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as pAkt degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Her2 degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Cdk6 degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as Raf degradation at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Change in Cdc37 expression in human MCF7 cells at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Change in p23 expression in human MCF7 cells at 5 times IC50 after 24 hrs by Western blot analysis 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP90 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP70 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
MCF7 Function assay 24 hrs Inhibition of HSP90 in human MCF7 cells assessed as induction of heat shock response-mediated HSP27 production at 5 times IC50 after 24 hrs by Western blot analysis relative to vehicle-treated control 25756299
PC12 Function assay 24 hrs Inhibition of D-aspartate biosynthesis in rat PC12 cells assessed as reduction of intracellular D-aspartate level up to 10 uM after 24 hrs by O-phthalaldehyde/N-acetyl-L-cysteine derivatization technique-based HPLC analysis 26642769
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in EGFR levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in Her2 levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
A549 Function assay 12 to 24 hrs Inhibition of Hsp90 in human A549 cells assessed as decrease in C-Raf levels at five times IC50 value after 12 to 24 hrs by Western blot analysis 26745854
BA/F3 Function assay 48 hrs Inhibition of imatinib-resistant BCR-ABL T315I mutant (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=1μM 26844689
BA/F3 Function assay 48 hrs Inhibition of imatinib-resistant BCR-ABL E255K mutant (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=1μM 26844689
BA/F3 Function assay 48 hrs Inhibition of BCR-ABL (unknown origin) expressed in mouse BA/F3 cells assessed as decrease in cell viability after 48 hrs by trypan blue exclusion assay, IC50=5μM 26844689
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTS/PMS assay, Activity=0.06μM 27003516
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay, IC50=0.03μM 27266997
HeLa Antiproliferative assay 72 hrs Antiproliferative activity against human HeLa cells after 72 hrs by SRB assay, IC50=0.06μM 27266997
SNU638 Anticancer assay 72 hrs Anticancer activity against human SNU638 cells measured after 72 hrs by SRB assay, IC50=0.03μM 27283788
Caki1 Anticancer assay 72 hrs Anticancer activity against human Caki1 cells measured after 72 hrs by SRB assay, IC50=0.056μM 27283788
A549 Anticancer assay 72 hrs Anticancer activity against human A549 cells measured after 72 hrs by SRB assay, IC50=0.109μM 27283788
MDA-MB-231 Anticancer assay 72 hrs Anticancer activity against human MDA-MB-231 cells measured after 72 hrs by SRB assay, IC50=0.11μM 27283788
HCT116 Anticancer assay 72 hrs Anticancer activity against human HCT116 cells measured after 72 hrs by SRB assay, IC50=0.15μM 27283788
SKBR3 Antiproliferative assay 1 to 3 days Antiproliferative activity against Her2-overexpressing human SKBR3 cells after 1 to 3 days by MTS assay, GI50=0.43μM 27783977
NCI-H1975 Antiproliferative assay 1 to 3 days Antiproliferative activity against gefitinib-resistant human NCI-H1975 cells after 1 to 3 days by MTS assay, GI50=0.56μM 27783977
RPMI8226 Growth inhibition assay 72 hrs Growth inhibition of human RPMI8226 cells after 72 hrs by MTS/PMS assay, GI50=0.003μM 29057043
MDA-MB-231 Antiproliferative assay 72 hrs Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay, IC50=0.06μM 29172085
A549 Antiproliferative assay 72 hrs Antiproliferative activity against human A549 cells after 72 hrs by SRB assay, IC50=0.1μM 29172085
NCI-H1975 Antiproliferative assay 72 hrs Antiproliferative activity against human NCI-H1975 cells after 72 hrs by Cell Titer 96 Aqueous One Solution cell proliferation assay, GI50=0.56μM 29202402
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of Raf1 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of CDK4 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of Her2 protein degradation incubated for 24 hrs by Western blot analysis 31591016
SKBR3 Function assay 24 hrs Inhibition of HSP90 at N-terminus in human SKBR3 cells assessed as induction of AKT protein degradation incubated for 24 hrs by Western blot analysis 31591016
Jurkat Function assay Inhibition of TNF-alpha-induced NF-kappaB activation expressed in FADD deficient human Jurkat cells by luciferase reporter gene assay 18408713
HEK293T Function assay Inhibition of TNF-alpha-induced NF-kappaB activation expressed in HEK293T cells by luciferase reporter gene assay 18408713
A549 Growth inhibition assay Growth inhibition of human A549 cells, GI50=0.064μM 18243703
KPL4 Growth inhibition assay Growth inhibition of human KPL4 cells, GI50=0.059μM 18243703
DLD1 Growth inhibition assay Growth inhibition of human DLD1 cells, GI50=0.037μM 18243703
HCT116 Growth inhibition assay Growth inhibition of human HCT116 cells, GI50=0.021μM 18243703
MCF7 Growth inhibition assay Growth inhibition of human MCF7 cells after days by SRB assay, GI50=35.6μM 17869098
LS174T Cytotoxicity assay Cytotoxicity against human LS174T cells by MTS assay, IC50=0.45μM 17034135
HEK293 Function assay Inhibition of HSP90 expressed in HEK293 cells assessed as effect on Akt1:p27 interaction complexes by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
SKBR-3 Function assay Effective concentration for Her-2 degradation in SKBR-3 cells, Concentration=5μM 15713410
MCF-7 Function assay Inhibition of steady-state expression of Raf-1 in MCF-7 breast cancer cells, IC50=0.2μM 10340605
MCF-7 Function assay Inhibition of steady-state expression of ER in MCF-7 breast cancer cells, IC50=0.06μM 10340605
MCF-7 Function assay Inhibition of steady-state expression of HER2 in MCF-7 breast cancer cells, IC50=0.05μM 10340605
SK-BR-3 Function assay In vitro inhibition of p185 erbB-2 oncoprotein in human breast cancer SK-BR-3 cells, IC50=0.07μM 7562912
HT-29 cell Growth inhibition assay Inhibitory concentration against human colorectal carcinoma HT-29 cell lines, IC50=24.5 nM 15658879
K562 cell Growth inhibition assay Inhibitory concentration against human leukemia K562 cell lines, IC50=22.1 nM 15658879
MCF-7 cell Growth inhibition assay Inhibitory concentration against human breast cancer MCF-7 cell lines, IC50=6.5 nM 15658879
SW620 cell Growth inhibition assay Inhibitory concentration against human colorectal carcinoma SW620 cell lines, IC50=6.2 nM 15658879
A2780 cells Proliferation assay Compound was evaluated for antiproliferative activity against human ovarian carcinoma cell line A2780, IC50=3.4 μM 11514145
SH-SY5Y Neuroprotection assay Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.04269μM 19138859
MCF7 Cytotoxicity assay Cytotoxicity against human MCF7 cells by SRB assay, IC50=9.6μM 19560353
SK-BR-3 Antiproliferative assay Antiproliferative activity against human SK-BR-3 cells, IC50=0.0158μM 19896848
MCF7 Antiproliferative assay Antiproliferative activity against human MCF7 cells, IC50=0.0161μM 19896848
HCT116 Antiproliferative assay Antiproliferative activity against human HCT116 cells by luminescence assay, EC50=0.03μM 21605975
U87 Antiproliferative assay Antiproliferative activity against human U87 cells by luminescence assay, EC50=0.089μM 21605975
HCT116 Antiproliferative assay Antiproliferative activity against human HCT116 cells, IC50=0.03μM 21715165
NCI-H1975 Antiproliferative assay Antiproliferative activity against human NCI-H1975 cells, IC50=0.036μM 21715165
U87MG Antiproliferative assay Antiproliferative activity against human U87MG cells, IC50=0.089μM 21715165
HepG2 Cytotoxicity assay Cytotoxicity against human HepG2 cells, IC50=0.37μM 22849774
HepG2 Cytotoxicity assay Cytotoxicity against human HepG2 cells by MTT assay, IC50=0.3μM 23656556
SKBR3 Function assay Inhibition of Hsp90 in human SKBR3 cells, IC50=0.07μM 26844689
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生物活性

产品描述 Geldanamycin是一种天然的HSP90抑制剂，Kd为1.2 μM，特异性干扰糖皮质激素受体(GR)/HSP联合。Geldanamycin 通过减少宿主的炎症反应来减轻病毒感染引起的ALI(急性肺损伤)/ARDS(急性呼吸窘迫综合征)。
靶点
p185 [4]
(SKBr3 cells)		 HSP90 (N-terminal domain) [1]
(Cell-free assay)		 HSP90 [1]
(Cell-free assay)
70 nM 0.78 μM(Kd) 1.2 μM(Kd)
体外研究(In Vitro)
体外研究活性 Geldanamycin结合于Hsp90s (残基1-220) N-末端结构域的ATP结合位点。Geldanamycin剂量依赖性抑制Hsp90的ATPase活性。[1] 在A2780人卵巢细胞系中，Geldanamycin引起剂量依赖性G2期阻滞，并可逆抑制其进入S期。该抑制作用伴随p53的增加，最终证明是p53依赖性的。[2] Geldanamycin引起p185受体蛋白-酪氨酸激酶的泛素化和蛋白酶体降解，IC50为70 nM。[3, 4] Geldanamycin是典型的抗肿瘤试剂，对一组60个人肿瘤细胞系的平均GI50为0.18 μM。[5]
激酶实验 核苷结合的等温滴定量热法(ITC)
滴定实验使用MSC系统进行。在每个实验中，16等份15 μL geldanamycin (300 μM在1% DMSO中)在25 °C下注射到1.3 mL蛋白质(31 μM在20 mMTris-HCl中, pH 7.5, 1 mMEDTA)，得到的数据减去稀释热后进行拟合。将geldanamycin加入缓冲液和缓冲液加入蛋白质的稀释热在单独实验中测定。在核苷酸结合位点没有观察到DMSO的结合。滴定数据使用非线性最小二乘法曲线拟合算法拟合，该算法有三个不固定变量：化学计量，结合常数(Kb) 1/Kd)，和相互作用焓的变化(ΔH°)。Geldanamycin结合到完整酵母Hsp90和Hsp90 N-末端结构域，估计的解离常数分别为1.22 μM和0.78 μM。结合到C-末端片段时，没有观察到有意义的热量。
细胞实验 细胞系 A2780人卵巢癌细胞系
浓度 0.001-10 μM
孵育时间 3小时
方法 指数生长的细胞用Geldanamycin处理，不同时期的DNA合成通过溴脱氧尿苷(BrdUrd)的整合和流式细胞分析术评估。在此期间，处理的和未处理的细胞，总细胞数没有显著的差别。BrdUrd (10 μM)在37 °C下4小时的培育期间进行整合，将细胞采集并固定在70%乙醇中。DNA通过2 N HC1变性后，细胞用抗- BrdUrd小鼠单克隆抗体培育，随后用异硫氰酸荧光素(FITC)-连接的山羊抗-小鼠IgG培育。细胞在室温下用碘化丙啶着色30分钟，使用Coulter EPICS Profile分析器通过流式细胞术分析。
体内研究(In Vivo)
体内研究活性 在FRE/erbB-2 小鼠体内，Geldanamycin (50 mg//kg)对pl85-相关的磷酸酪氨酸水平表现出30%的抑制。[6]
动物实验 Animal Models 负荷FRE/erbB-2肿瘤的nu/nu小鼠
Dosages 50 mg/kg
Administration 通过腹腔注射给药
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00003969 Completed
Unspecified Adult Solid Tumor Protocol Specific
Cancer Research UK|National Cancer Institute (NCI)
 August 1998 Phase 1

化学信息&溶解度

分子量 560.64 分子式

C29H40N2O9
CAS号 30562-34-6 SDF Download Geldanamycin SDF
Smiles CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC
储存条件(自收到货起)

体外溶解度
批次：

DMSO : 33 mg/mL ( (58.86 mM)； DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

 动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

mg/kg g μL 只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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