Honokiol (NSC 293100)

For research use only. Not for use in humans.

目录号:S2310 中文名称:和厚朴酚

Honokiol (NSC 293100) Chemical Structure

CAS No. 35354-74-6

Honokiol (NSC 293100)是木兰的有效成分,抑制Akt磷酸化,且促进ERK1/2磷酸化。Honokiol 可造成 G0/G1 期阻滞,并可通过 ROS/ERK1/2 信号通路来诱导凋亡和自噬。Honokiol 可抑制 hepatitis C virus (HCV) 感染。Phase 3。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 899.31 现货
RMB 716.85 现货
RMB 1208.43 现货
RMB 2117.23 现货
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客户使用Selleck生产的Honokiol (NSC 293100)发表文献23篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Honokiol (NSC 293100)是木兰的有效成分,抑制Akt磷酸化,且促进ERK1/2磷酸化。Honokiol 可造成 G0/G1 期阻滞,并可通过 ROS/ERK1/2 信号通路来诱导凋亡和自噬。Honokiol 可抑制 hepatitis C virus (HCV) 感染。Phase 3。
靶点
Akt-phosphorylation [1] MEK [8]
()
体外研究

Honokiol作用于黑色素瘤,肉瘤,骨髓瘤,白血病,膀胱癌,肺癌,前列腺癌,口腔鳞状细胞癌和结肠癌细胞系,具有促细胞凋亡的作用效果。Honokiol作用于 SVR肉瘤细胞,有效诱导细胞凋亡。Honokiol处理SVR细胞,降低MAP, akt, 和c-src的磷酸化。另外,Honokiol增强TRAIL调节的凋亡,且通过TRAIL中和抗体可部分废除Honokiol毒性。Honokiol也具有直接的抗血管生成活性, 因为VEGF-VEGFR2的相互作用,Honokiol阻断磷酸化和rac激活[1] 通过激活caspase 8,再激活caspase 9 和3,Honokiol促进CLL细胞凋亡。Honokiol抑制白细胞介素-4调节的CLL细胞生存,也增强Chlorambucil, Fludarabine, 和Cladribine的细胞毒性。[3] Honokiol可以杀死复发患者的骨髓瘤细胞,但是这种剂量不会杀死外周血单个核细胞(PBMCs)。Honokiol 处理,诱导Caspase 3, 7, 8, 和 9,及 PARP裂解。 [2] Honokiol 作用于结肠癌细胞RKO,诱导凋亡。[4]Honokiol 通过调节NF-κB活化途径,增强细胞凋亡,抑制破骨细胞,并抑制入侵。[5] Honokiol可作为一种有效的抗炎试剂,因其具有抑制PI3K/Akt通路的作用,Honokiol具有多能活性。[6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human UACC-903 cells MlezR5l1d3SxeHnjbZR6KGG|c3H5 MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVRWNENTlyMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVUvOSEQvF2= NYjnc|lGOjJ3M{O5PFM>
Vero E6 cells NHrZTGNHfW6ldHnvckBie3OjeR?= M1GzeGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHNCWlNiY3;yc45ifmm{dYOgbY4hXmW{bzDFOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHL5JGVNUVODLDDFR|UxRTZwNTFOwG0> NVnpbFZHOTd4NkO1N|k>
human UACC-903 cells Mo\MR5l1d3SxeHnjbZR6KGG|c3H5 M{DSU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0NvOUCzJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:Py52NTFOwG0> Mo\pNlI2OzN7OEO=
human A549 cells MlzpR5l1d3SxeHnjbZR6KGG|c3H5 NE[5S4hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9O{44PSEQvF2= M3OwOlIzPTN|OUiz
human CEM cells NIX4Xo9EgXSxdH;4bYNqfHliYYPzZZk> M{TLcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{whUUN3ME2xNE46KM7:TR?= NYDKRYFwOTd3OEe1O|I>
HEK293 cells NXLIRWdlTnWwY4Tpc44h[XO|YYm= M3joZmFod26rc4SgZYN1cX[rdImgZZQhWliUYXzwbIEhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGGodHXyJFQ5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OTFwODFOwG0> NITIcGYzODZ7NUS3Ni=>
human A549 cell M3e2VWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmLiNlQhcA>? NIPlfXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NVIvPTFizszN M{ftNVIzPTN|OUiz
human HT-29 cells NVfHRnNkS3m2b4TvfIlkcXS7IHHzd4F6 NIi1VZc4OiCq M4O4b2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NVMvOjRizszN NF:zO|gzOjV|M{m4Ny=>
human PBM cells MUDDfZRwfG:6aXPpeJkh[XO|YYm= NEnhSpBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSk1iY3XscJMtKEmFNUC9NVYvOSEQvF2= NHrMOIYyPzV6N{W3Ni=>
Hep-G2 cells NWnQU3I6S3m2b4TvfIlkcXS7IHHzd4F6 M1jtXWN6fG:2b4jpZ4l1gSCxZjDjc41xd3WwZDDh[4FqdnO2IHj1cYFvKGyrdnXyJJR2dW:{IHPlcIwhdGmwZTCoTIVxNUd{KTD3ZZMh\GW2ZYLtbY5m\CxiSVO1NF0yPi53IN88US=> NVKyXJVTOTV3OEK0N|I>
human HepG2 cells M2XkPHBzd2yrZnXyZZRqd25iYYPzZZk> MmTUNlQhcA>? NHO2THpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MU[uOUDPxE1? NXq3TZY1OjF6NUO5PVE>
human K562 cells NWrmPYczWHKxbHnm[ZJifGmxbjDhd5NigQ>? MkLwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME2yNU4yKM7:TR?= MWWxPVU5QTZ5OB?=
Vero cells MV;DfZRwfG:6aXPpeJkh[XO|YYm= Mn3mR5l1d3SxeHnjbZR6KGGpYXnud5QhXmW{bzDj[YxteyxiSVO1NF0zOi53IN88US=> NHmyV3gyPjd{Mk[2OC=>
human A2780 cells M{SzdHBzd2yrZnXyZZRqd25iYYPzZZk> NV3Wd2RXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBkcXOybHH0bY4ue2Wwc3n0bZZmKGi3bXHuJGEzPzhyIHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OD1|MD61JO69VQ>? NETG[W0yQTV6OU[3PC=>
human SPC-A1 cells NED0b2xRem:uaX\ldoF1cW:wIHHzd4F6 Mk\kRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVUFOtRVEh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM3NjFizszN M3\zWFE6PTh7Nke4
HUVEC cells M2jrbGZ2dmO2aX;uJIF{e2G7 NIXVNXgzOCEQvF2= MmXFNlQhcA>? NU\BVWt5SW62aX3p[5JifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiXVlXDJINmdGy|IHH0JFIxKHWPIHHmeIVzKDJ2IHjyd{BjgSC5b4Xu[E1p\WGuaX7nJIF{e2G7 NVzwXIU1OjF6NUO5PVE>
HEK293 cells NIHWSIlHfW6ldHnvckBie3OjeR?= MkLDNlQuPDhiaB?= MWTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGLYVk1idHCqYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPv[ZhxemW|c3nu[{B4cXSqIIDDUXgu[mW2YT3nZYwh[W[2ZYKgNlQhfG9iNEigbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> M1TlW|I1QTV7OUi3
human SH-SY5Y cells Moj2SpVv[3Srb36gZZN{[Xl? Mn7PNVAh|ryP NWDkSmpsOzBibXnudy=> M3LDVG5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBpfW2jbjDTTE1UYTW\IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS0iSIHHu[EBVSkiSLXnu[JVk\WRiY3XscEBl\WG2aDDheEAyOCC3TTDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMheHKrb4KgeI8h[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFMhcHK|IHL5JG1VXCCjc4PhfS=> NY\JTIJvOjJzNEK1N|k>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-EGFR / p-AKT / p-STAT3 / p-ERK / p-GSK3α/β / Bax / p-pRb / p-BAD / IκBα / CDK2 / CDK4 / Cyclin D1; 

PubMed: 27458163     


Honokiol differentially modulated the level of EGFR and its downstream effectors in 1170 cells in a dose-dependent manner. Cells were treated with the different concentrations of honokiol for 72 h, and cell lysates were analyzed by Western immunoblotting as described in Material and Methods. At least three independent assays were carried out using cell lysates prepared on different days.

p-Lyn / Lyn / p-PI3K ; 

PubMed: 29892225     


Lyn, p-Lyn, EGFR, p-EGFR, PI3K, p-PI3K, AKT, p-AKT, STAT3, and p-STAT3 protein expression of PC-9 cells were detected by Western blots assay after treated with honokiol (0, 20, 40, and 60 μM) for 24 h. The non-specific Src tyrosine kinase inhibitor (TKI) PP2 is the positive control. ∗P > 0.05 and ∗∗∗P < 0.01 for comparison among groups.

27458163 29892225
Immunofluorescence
Vimentin / Occludin ; 

PubMed: 24508063     


MCF7 and MDA‐MB‐231 cells were treated with vehicle (C) or 5 μM honokiol (HNK) and subjected to immunofluorescence analysis of vimentin and occludin.

IκBα / NF-κB p65 ; 

PubMed: 27212040     


Confocal microscopy of localization and expression of IκBα (Red) and p65 (green) in ECs preincubated with DMSO or honokiol (3 μM) for 30 min, then with TNF-α (10 ng/ml) for 15 min. Bars, 10 μm. Representative images from 3 or 4 experiments are shown.

24508063 27212040
Growth inhibition assay
Cell viability; 

PubMed: 30587839     


Effect of honokiol on glioma/glioblastoma cell viability. U251 human glioma and U-87 MG human glioblastoma cells were treated with different concentrations of honokiol for 24 (A) and 48 h (B). MTT assay was used to assess cell viability. Data are presented as means ± SEM from 3 independent experiments. IC50 values for U251 and U-87 MG at 24 h were 54 μM and 62.5 μM, respectively. * p < 0.05 and ** p < 0.01 vs. vehicle control (one-way ANOVA with Tukey's post-hoc test).

30587839
体内研究

Honokiol处理裸鼠,高效抗SVR肉瘤。[1]Honokiol 作用于小鼠移植瘤,抑制RKO细胞生长。[4] Honokiol 处理小鼠移植瘤,抑制MDA-MD-231乳腺癌细胞生长。[7]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 53 mg/mL (198.99 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
2mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 266.334
化学式

C18H18O2

CAS号 35354-74-6
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID