Decitabine (NSC 127716)

For research use only. Not for use in humans.

目录号:S1200 别名: Deoxycytidine, Dacogen, 5-aza-2'-deoxycytidine, 5-AZA-dC, 5-aza-CdR 中文名称:地西他滨

Decitabine (NSC 127716) Chemical Structure

CAS No. 2353-33-5

Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR)是DNA甲基转移酶抑制剂,整合进入DNA、导致DNA低甲基化,并使DNA复制停滞在intra-S-phase。Decitabine被用于治疗骨髓增生异常综合征(MDS)。Decitabine可在多种癌细胞系中诱导细胞周期阻滞和凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1269.11 现货
RMB 732.42 现货
RMB 1419.77 现货
RMB 2621.82 现货
RMB 4679.47 现货
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客户使用Selleck生产的Decitabine (NSC 127716)发表文献107篇:

产品安全说明书

DNA Methyltransferase抑制剂选择性比较

生物活性

产品描述 Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR)是DNA甲基转移酶抑制剂,整合进入DNA、导致DNA低甲基化,并使DNA复制停滞在intra-S-phase。Decitabine被用于治疗骨髓增生异常综合征(MDS)。Decitabine可在多种癌细胞系中诱导细胞周期阻滞和凋亡。
特性 Decitabine 是DNA甲基化的有效抑制剂。
靶点
DNA methylation [1]
(HL-60, KG1a cells)
体外研究

Decitabine 有效抑制DNA 合成,这种作用存在剂量依赖性,作用于HL-60和 KG1a 白血病细胞时,IC50分别为 100 ng/mL 和1 ng/mL。Decitabine 抑制细胞生长,这种作用存在剂量和时间依赖性,处理HL-60和KG1a白血病细胞72小时和96小时,IC50分别约为100 ng/mL 和 10 ng/mL。[1] 最新研究显示Decitabine作用于间变性大细胞淋巴瘤(ALCL),具有抗增殖和促凋亡活性,且抑制KARPAS-299细胞中[3H]胸甘的摄取,EC50 为0.49 μM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Eca109 MlnySpVv[3Srb36gRZN{[Xl? MUGwMlXDqM7:TR?= M1niNFI1KGh? NHPk[5J4[XSnch?= Ml;mcY9lfWyjdHXzJJRp\SCpZX7lJIV5eHKnc4Ppc44hd2ZiTVHHSU1CKG2nbXLldpM> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{M{C4Nkc,OjVzMkOwPFI9N2F-
Eca109 NEPxdG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHJVXoxNjVxMj61M|Uh|ryP NH\vVJYzPC92OD:3NkBp NFPWbI14[XSnch?= NIf1fpBqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy NF7rPIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN|A5Oid-MkWxNlMxQDJ:L3G+
Eca109 NW\nXZRMTnWwY4Tpc44hSXO|YYm= NWPWOlZ3OC53wrFOwG0> MVm2M|EzNzJ2IHi= MkKwe4F1\XJ? NInaVWRqdmirYnn0d{Bk\WyuIH3p[5JifGmxbtMg MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{M{C4Nkc,OjVzMkOwPFI9N2F-
Eca109 MXfGeY5kfGmxbjDBd5NigQ>? M3zwVFAvPcLizszN MoXiNlQhcA>? M17RcJdifGW{ NVe1c3JPcW6qaXLpeJMh[2WubDDpcpZie2mxbh?= MmnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTwNE42yqEQvF2= Mo\kNlQhcA>? MmDne4F1\XJ? M{TTeIlv\HWlZYOgS|IwVSCjcoLld5QhcW5idHjlJINmdGxiY4njcIU> MlvNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109 NWr2clN[TnWwY4Tpc44hSXO|YYm= MXSwMlUwOSEQvF2= M2\ORVI1KGh? NIfZb2p4[XSnch?= MXHk[YNz\WG|ZYOg[ZhxemW|c3nvckBw\iCQRj5OvmIzKGGwZDDNUXAz NYPjdFF1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlMxQDJpPkK1NVI{ODh{PD;hQi=>
SW1116  MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnGwNE42NzFxMj:1JO69VQ>? M4TSeVQ5KGh? MkPJSG1UVw>? NWTENYd{\W6qYX7j[ZMhfGinIFfl[ol1cW6rYjDpcoR2[2WmIHPlcIwhcW6qaXLpeIlwdg>? NXPBOW5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O|QzQDZpPkK0PFc1Ojh4PD;hQi=>
LOVO NVjKSIV[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWmwMlUwOS9{L{Wg{txO NH7NXGI1QCCq MVvEUXNQ Mont[Y5p[W6lZYOgeIhmKEenZnn0bY5q[iCrbnT1Z4VlKGOnbHygbY5pcWKrdHnvci=> NF24NpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O|QzQDZ:L3G+
SW1116  MXXGeY5kfGmxbjDBd5NigQ>? MXexNEDPxE1? M4XXWVQ5KGh? NEL2ZpRFVVOR NF76bGpqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ? NED1bpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O|QzQDZ:L3G+
LOVO NFrmXmNHfW6ldHnvckBCe3OjeR?= MUOxNEDPxE1? MnnKOFghcA>? M4HRS2ROW09? NGPXTXRqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ? NUCzU|NSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O|QzQDZpPkK0PFc1Ojh4PD;hQi=>
SW1116  M1TXWmFxd3C2b4Ppd{BCe3OjeR?= MlG2NVAh|ryP MXS0PEBp M33QbmROW09? NYjVU5Jn\W6qYX7j[ZMhT2WoaYTpcoljNWmwZIXj[YQh[XCxcITvd4l{ M{jmN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
LOVO M1PPNGFxd3C2b4Ppd{BCe3OjeR?= NHXxOpoyOCEQvF2= MVS0PEBp MoLVSG1UVw>? M3;odoVvcGGwY3XzJGdm\mm2aX7pZk1qdmS3Y3XkJIFxd3C2b4Ppdy=> M33SdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
RPMI-8226 NHGzZoRCeG:ydH;zbZMhSXO|YYm= NGXnN|AyNzJizszN MVW0PE84Oi97NjDo MXTEUXNQ NI\LZY5qdmS3Y3XzJINmdGxiYYDvdJRwe2m| MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
OPM-2  MUfBdI9xfG:|aYOgRZN{[Xl? NX\0e|I4OS9{IN88US=> MWq3Nk86Pi9zMkCgbC=> M{DPRWROW09? NFXqNYZqdmS3Y3XzJINmdGxiYYDvdJRwe2m| MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
JJN3  NWX1e29nSXCxcITvd4l{KEG|c3H5 NXHLWWU2OC53L{Gg{txO MWeyOE81QCCq M{\Sd2ROW09? NHGzWW9qdmS3Y3XzJINmdGxiYYDvdJRwe2m| NYDOVXNpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N|MyODhpPkK0PFM{OTB6PD;hQi=>
NCI-H929  MXrBdI9xfG:|aYOgRZN{[Xl? M4TmOlEwOiEQvF2= MXG3Nk86Pi9zMkCgbC=> NWLGfJBJTE2VTx?= MXTpcoR2[2W|IHPlcIwh[XCxcITvd4l{ NUnxSnZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N|MyODhpPkK0PFM{OTB6PD;hQi=>
RPMI-8226 NIm0R4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF2xcmwyNzJizszN NWH4NY8{OjRxNEivO|IhcA>? NFuySJdFVVOR M1jpeIFn\mWldIOgZ4VtdCCleXPs[UBxem:pcnXzd4lwdiCwZXfheIl3\Wy7 NXjpOGhKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N|MyODhpPkK0PFM{OTB6PD;hQi=>
OPM-2  MoDFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jHeVEwOiEQvF2= NUW3bmp4OjRxNEivO|IhcA>? MUHEUXNQ NVTYdVFK[W[oZXP0d{Bk\WyuIHP5Z4xmKHC{b3fy[ZN{cW:wIH7l[4F1cX[nbIm= MmPWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6M{OxNFgoRjJ2OEOzNVA5RC:jPh?=
JJN3  NX3l[pU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHO5UW8xNjVxMTFOwG0> M{jxcFI1NzR6L{eyJIg> MXfEUXNQ NV:4R40{[W[oZXP0d{Bk\WyuIHP5Z4xmKHC{b3fy[ZN{cW:wIH7l[4F1cX[nbIm= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
NCI-H929  MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV2xM|Ih|ryP MofQNlQwPDhxN{KgbC=> NIWz[mJFVVOR MorFZYZn\WO2czDj[YxtKGO7Y3zlJJBzd2e{ZYPzbY9vKG6nZ3H0bZZmdHl? NG\UVYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN|ExQCd-MkS4N|MyODh:L3G+
HeLa MmPWT4lv[XOnIFHzd4F6 Mlz6T4k:OTByMPMAl|UxODBizszNJIZweiCqRV7UNS=> M2\ObVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa NF3K[nhMcW6jc3WgRZN{[Xl? MYjLbV02NjZiwsGgNE42KM7:TTDmc5IhcEWQVEK= M3;OeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa MUTLbY5ie2ViQYPzZZk> Mn3hT4k:OjFwNjFCtUA{NjBizszNJIZweiCqQ17UNS=> M2PjR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa NV74fFNEU2mwYYPlJGF{e2G7 MoLIT4k:OTRwNDFCtUA1NjZizszNJIZweiCqQ17UNy=> NF3CZnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe4NFA6QCd-MkS3PFAxQTh:L3G+
NB4 NFPWeY1HfW6ldHnvckBCe3OjeR?= NX7OPI1FOi53L{WvO{42NzFyIN88US=> MoK5NlQhcA>? M1XVUWROW09? MmXibY5kemWjc3XhJJRp\SCneIDy[ZN{cW:wIH;mJJBz\WO3coPvdkBucVJvMUK1ZS=> MmnJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OES4O|AoRjJ2NEi0PFcxRC:jPh?=
CD4+ CD25− T  MofmSpVv[3Srb36gRZN{[Xl? NH;rNmwyNzVizszN MV7y[YR2[2WVIHfsc4JidCCGTlGgcYV1cHmuYYTpc44> NVn1W5VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|Y{PjBpPkK0OFc3OzZyPD;hQi=>
BV-173 MXTBdI9xfG:|aYOgRZN{[Xl? M{XuTVAvOjVxMD61M|AvPzVxMTFOwG0> M1jU[lQ5Nzd{L{m2JIg> MmOzxsBRSlN? NFPsNI1qdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> MkmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkO2NVMoRjJ2NEKzOlE{RC:jPh?=
ML-1 M4fSe2Fxd3C2b4Ppd{BCe3OjeR?= M3riZlAvOjVxMD61M|AvPzVxMTFOwG0> NX;oTW9OPDhxN{KvPVYhcA>? NHz2UWPDqFCEUx?= NHf3bXNqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HL-60 MoTnRZBweHSxc3nzJGF{e2G7 NH:4TJcxNjJ3L{CuOU8xNjd3L{Gg{txO M2C1O|Q5Nzd{L{m2JIg> MkLYxsBRSlN? MULpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHLveIgh\G:|ZT2gZY5lKHSrbXWtJIRmeGWwZHXueEBu[W6wZYK= NW\CbW5YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlM3OTNpPkK0OFI{PjF|PD;hQi=>
KG-1a NFLsV3lCeG:ydH;zbZMhSXO|YYm= MX[wMlI2NzBwNT:wMlc2NzFizszN NH;iWXc1QC95Mj:5OkBp NYHL[Xd6yqCSQmO= M3vveIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=> NHzaNlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyN|YyOyd-MkS0NlM3OTN:L3G+
BV-173 M3KzVGZ2dmO2aX;uJGF{e2G7 M{jsWVI2OC93MEDuUS=> MUK0PEBp MWlCpHBDWw>? MWnpcoR2[2W|IHTlcIF6\WRiYX7kJJN2e3SjaX7l[EBTV1NiaX7jdoVie2V? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
CEM NGC4U5VHfW6ldHnvckBCe3OjeR?= Mom2NlUxNzVyMH7N NIjuXYQ1QCCq MXdCpHBDWw>? MWnpcoR2[2W|IHTlcIF6\WRiYX7kJJN2e3SjaX7l[EBTV1NiaX7jdoVie2V? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HL-60 NWP1SFdrTnWwY4Tpc44hSXO|YYm= M1ezWFI2OC93MEDuUS=> MVi0PEBp NWm1TVRMyqCSQmO= MoHlbY5lfWOnczDk[YxigWWmIHHu[EB{fXO2YXnu[YQhWk:VIHnuZ5Jm[XOn NUDiT2FtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlM3OTNpPkK0OFI{PjF|PD;hQi=>
ML-1 M4LyNmZ2dmO2aX;uJGF{e2G7 NHm0[3ozPTBxNUCwcm0> NVfqO|VGPDhiaB?= NHToV|LDqFCEUx?= M2rjdIlv\HWlZYOg[IVt[XmnZDDhcoQhe3W|dHHpcoVlKFKRUzDpcoNz\WG|ZR?= M3vMUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN|YyOzxxYU6=
DLD-1 MUPGeY5kfGmxbjDBd5NigQ>? M1[4UVI2OC93MEDuUS=> NGHlWXE1QCCq M1zUbeKhWEKV MljM[I8hdm:2IHnu[JVk\XNiZHXsZZlm\CCjbnSgd5V{fGGrbnXkJHJQWyCrbnPy[YF{\Q>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HCT-116 NEXodppHfW6ldHnvckBCe3OjeR?= NGnTdWkzPTBxNUCwcm0> NEnpOoo1QCCq MnLGxsBRSlN? M3;JfYRwKG6xdDDpcoR2[2W|IHTlcIF6\WRiYX7kJJN2e3SjaX7l[EBTV1NiaX7jdoVie2V? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
U937-A/E-9/14/18  M3zhb2Fxd3C2b4Ppd{BCe3OjeR?= NXfk[4Z{OC5yMT:wMlEwOS9zMDFOwG0> NYDwPXE4PDhiaB?= M13MWolv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M1TtN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{CwOFU3Lz5{NEOwNFQ2PjxxYU6=
HT29 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\lO|IhcA>? NG\T[nhKSzVyPUG0NFDDuTF5OTFOwG0> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5MkC2NUc,OjRzN{KwOlE9N2F-
SW48 NXH1fWFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvGSZV[PzJiaB?= NF:0e3VKSzVyPUG1MlLDuTZwMjFOwG0> M1fL[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUeyNFYyLz5{NEG3NlA3OTxxYU6=
HCT116 M{ixXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\aO|IhcA>? NH63WFNKSzVyPUGuO:KyOC52IN88US=> NULtbVVDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|IxPjFpPkK0NVczODZzPD;hQi=>
HepG2 NFfidpZHfW6ldHnvckBCe3OjeR?= Ml3CNE42NzFizszN MmnINlQhcA>? MXjEUXNQ NF\2S4F2eC2{ZXf1cIF1\WRidHjlJJJmdGG2aY\lJG9EXE5{IH3SUmEh[W6mIIDyc5RmcW5iZYjwdoV{e2mxbh?= M4j6VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS2PFc1Lz5{NEG0Olg4PDxxYU6=
LS174T NIj2N5dHfW6ldHnvckBCe3OjeR?= M1T0dlAvPS9zIN88US=> MmLTNlQhcA>? NEDhPIdFVVOR MXfs[YFlKHSxIHHuJIlv[3KnYYPlJI9nKE:FVF6yJIxmfmWucx?= M4\GZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS2PFc1Lz5{NEG0Olg4PDxxYU6=
HepG2 MWPBdI9xfG:|aYOgRZN{[Xl? MYKxM|ExNzFyMDFOwG0> MmP2O{Bl NEnlPFdFVVOR MWXpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M4O2dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS2PFc1Lz5{NEG0Olg4PDxxYU6=
LS174T MV3BdI9xfG:|aYOgRZN{[Xl? NH\2[VIyNzFyL{GwNEDPxE1? MX:3JIQ> MVnEUXNQ NG\0XYVqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NFTWSoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0Olg4PCd-MkSxOFY5PzR:L3G+
QBC-939 M3e0bGFxd3C2b4Ppd{BCe3OjeR?= NYnmSoFlOS9zMD:xNFAh|ryP M1flRVch\A>? MlrGSG1UVw>? MVXpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2Nki3OEc,OjRzNE[4O|Q9N2F-
U251 M3r3N2Fxd3C2b4Ppd{BCe3OjeR?= NELBUZYyNzFyL{GwNEDPxE1? Mlq1O{Bl MljGSG1UVw>? MYPpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NILH[mk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0Olg4PCd-MkSxOFY5PzR:L3G+
HL-60 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHybZUyKM7:TR?= NFjLZnk1QCCq NVu5SYxncW6lcnXhd4V{KEd{LYDoZZNmKGOnbHyg[pJi[3Srb36= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByMEOyOEc,OjRyMECzNlQ9N2F-
MDA‑MB‑453 NVTubllnTnWwY4Tpc44hSXO|YYm= M3u2ZlAvOi9zIN88US=> M2j4flczKGh? MV3jZZV{\XNicnWt[ZhxemW|c3nvckBw\iClbHH1[IlvyqBzwrC= NX\uRYxnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFQzOjhpPkKzPFQ1OjJ6PD;hQi=>
HCC1569 NF[0c4pHfW6ldHnvckBCe3OjeR?= MoLaNE4zNzFizszN M4rTXVczKGh? MlTWZ4F2e2W|IILlMYV5eHKnc4Ppc44hd2ZiY3zheYRqdsLiMdMg NEG0R289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OFIzQCd-MkO4OFQzOjh:L3G+
BT‑474 MnLxSpVv[3Srb36gRZN{[Xl? M{jzPVAvOi9zIN88US=> M2XNRVczKGh? MXrjZZV{\XNicnWt[ZhxemW|c3nvckBw\iClbHH1[IlvyqBzwrC= MkTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NESyNlgoRjJ|OES0NlI5RC:jPh?=
AGS NVPYO4c5SXCxcITvd4l{KEG|c3H5 MlfCOU8yOC9{MD:1NEDPxE1? NGfLT5g1QMLiaNMg MXjEUXNQ Mnq5bY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5xsA> NV7Qc|k3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PFI4QDRpPkKzOVgzPzh2PD;hQi=>
A549 NI\Qe5NCeG:ydH;zbZMhSXO|YYm= Ml:xOU8yOC9{MD:1NEDPxE1? MWm0POKhcMLi MmTFSG1UVw>? MWnpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdIpCpC=> NGCweY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W4Nlc5PCd-MkO1PFI4QDR:L3G+
AGS  MlzUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4m1NVUwOTBxMkCvOVAh|ryP MnrHOFjDqGkEoB?= NUHiRXg2TE2VTx?= MYTpcoR2[2W|IFeyM20heGijc3WgZZJz\XO2wrC= Mm\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEK3PFQoRjJ|NUiyO|g1RC:jPh?=
Kasumi-1 M13HPGFxd3C2b4Ppd{BCe3OjeR?= MmG5NE42KM7:TR?= NVfSZlE{PDkEoHlCpC=> M{To[oRm[3KnYYPld{B1cGViY3XscEB3cWGkaXzpeJkh[29vdILlZZRm\CC5aYToJHRnNU6SLYPj NYD6eo5SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM{PDhpPkKzOFk{OzR6PD;hQi=>
OCI-AML3 NXjB[2RJSXCxcITvd4l{KEG|c3H5 NEnwSZAzNjVizszN NUe1[Gh3PDkEoHlCpC=> M2nLd4Rm[3KnYYPld{B1cGViY3XscEB3cWGkaXzpeJkh[29vdILlZZRm\CC5aYToJHRnNU6SLYPj MnvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUOzOFgoRjJ|NEmzN|Q5RC:jPh?=
MV4-11 Mn7DRZBweHSxc3nzJGF{e2G7 MUCyMlUh|ryP MoHPOFjDqGkEoB?= NWDLSW52\GWlcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClbz30doVifGWmIIfpeIghXGZvTmCtd4M> NUOwOVFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM{PDhpPkKzOFk{OzR6PD;hQi=>
NK  MXfDfZRwfG:6aYT5JGF{e2G7 NHWyeYcxNjB{LUKwJO69VQ>? Mmj2OUBl NI\MZVJl\WO{ZXHz[ZMhfGinIHP5eI9tgXSrYzDhZ5Rqfmm2eTDv[kBPUyClZXzsd{BifCCrboTldo1m\GmjdHWgZ49v[2WwdILheIlwdnNicnXzeYx1cW6pIHnuJIEhXS2|aHHw[YQh\G:|ZfMAl5Jme3CxboPlJIN2en[n M1fCb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{K4NFg5Lz5{M{OyPFA5QDxxYU6=
NK  M{X5OGFxd3C2b4Ppd{BCe3OjeR?= MWmwMlAzNTJyIN88US=> MnPROUBl NGXC[m5l\WO{ZXHz[UBPUyClZXzsJJBzd2yrZnXyZZRqd25iYX7kJJZq[WKrbHn0fUBieyC2aHWgZ49v[2WwdILheIlwdiCrbnPy[YF{\WR? NWPmbJBURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNlgxQDhpPkKzN|I5ODh6PD;hQi=>
NK  Mnv0SpVv[3Srb36gRZN{[Xl? NWf0eXo5OC5yMT2yNEDPxE1? NU\SWlhQPSCm MYrjZZV{\XNiaInwc41mfGi7bHH0bY9vKG:oIF7LJINmdGy|IHnuJIEh\G:|ZfMAl5Jme3CxboPl Ml\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MkiwPFgoRjJ|M{K4NFg5RC:jPh?=
MOLT4/DNR Mlz3SpVv[3Srb36gRZN{[Xl? NGrVWWc2KM7:TR?= MVO0JIQ> M1;zRZJm\HWlZYOgRWJESjFibWLORUBmgHC{ZYPzbY9v NIXXe4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NFU4OCd-MkOwOlA2PzB:L3G+
Jurkat/DOX M2LpVGZ2dmO2aX;uJGF{e2G7 MkfROUDPxE1? MVW0JIQ> M1\Nb5Jm\HWlZYOgRWJESjFibWLORUBmgHC{ZYPzbY9v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MEW3NEc,OjNyNkC1O|A9N2F-
MOLT4/DNR NHv3RYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVq1JO69VQ>? NVLuWG5iPCCm MYjy[YR2[2W|IITo[UBKSzVywrD2ZYx2\SCob4Kg[IF2dm:{dXLpZ4lvKHOnboPpeIl3cXS7 Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkC1O|AoRjJ|ME[wOVcxRC:jPh?=
Jurkat/DOX NXfDOo5iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M36xflUh|ryP M{jKNlQh\A>? NYfoe442emWmdXPld{B1cGViSVO1NOKhfmGudXWg[o9zKGSjdX7vdpVjcWOrbjDz[Y5{cXSrdnn0fS=> NXPUbVNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
ccRCC  NETD[YxCeG:ydH;zbZMhSXO|YYm= M1fw[VAvODFvMUFOwG0> MnvuO|IhcA>? M2C5UWROW09? M37MbIhieyCvaX7pcYFtKGWoZnXjeEBwdiClZXzsJJBzd2yrZnXyZZRqd25? NVjp[415RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
TNBC  MV3BdI9xfG:|aYOgRZN{[Xl? MVqwMlAyNTFyzszN NUPvcGhVPzJiaB?= Mo\mSG1UVw>? Moq5bIF{KG2rbnntZYwh\W[oZXP0JI9vKGOnbHygdJJwdGmoZYLheIlwdg>? M4TZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK2OFY4Lz5{MkiyOlQ3PzxxYU6=
A498 NVSwOmVESXCxcITvd4l{KEG|c3H5 Mn3LNE4xOS1zMN88US=> MmHQO|IhcA>? M4\BOGROW09? MkLWbY5lfWOnczDzfY5memerc4TpZ{Bz\XOyb37z[ZPDqHerdHigdo9ucWSncIPpci=> NUPMbVlYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
KIJ265T M2i1SWFxd3C2b4Ppd{BCe3OjeR?= NXrzbWU5OC5yMT2xNO69VQ>? NWW1UpJ2PzJiaB?= NFnWUFZFVVOR M2DlPYlv\HWlZYOgd5lv\XKpaYP0bYMhemW|cH;ud4V{yqC5aYToJJJwdWmmZYDzbY4> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
MDA-231 NEnVcG1CeG:ydH;zbZMhSXO|YYm= MV6wMlAyNTFyzszN Mn7WO|IhcA>? Mn\ESG1UVw>? Mk[wbY5lfWOnczDzfY5memerc4TpZ{Bz\XOyb37z[ZPDqHerdHigdo9ucWSncIPpci=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
BT-20 MY\BdI9xfG:|aYOgRZN{[Xl? MYqwMlAyNTFyzszN NET5[Iw4OiCq NIqwcYpFVVOR MWTpcoR2[2W|IIP5coVz\2m|dHnjJJJme3CxboPld:Khf2m2aDDyc41q\GWyc3nu MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
U937 M1v4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoDNOU0zOCEQvF2= NYjtV5NbOjRxNEivO|IhcA>? MVnpcoR2[2W|IHGg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY4h[SClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy M1jNVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{[3NFIyLz5{Mke2O|AzOTxxYU6=
HL60 NUXPdXhyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUi1MVIxKM7:TR?= NIfhWGgzPC92OD:3NkBp MVjpcoR2[2W|IHGg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY4h[SClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NHqxdXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2O|AzOSd-MkK3OlcxOjF:L3G+
U937 NGKzdZJCeG:ydH;zbZMhSXO|YYm= NGTWNnIyPSEQvF2= M3jHSlI1NzR6L{eyJIg> MnT0bY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NVzsT25uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OlcxOjFpPkKyO|Y4ODJzPD;hQi=>
HL60 NFz5W3JCeG:ydH;zbZMhSXO|YYm= MVOxOUDPxE1? NU\T[JpHOjRxNEivO|IhcA>? MX\pcoR2[2W|IHPlcIwh[XCxcITvd4l{ MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd4N{CyNUc,OjJ5NkewNlE9N2F-
LS411N  M4fvT2Fxd3C2b4Ppd{BCe3OjeR?= NHWxS2MxNjVizszN M1zKbFczKGh? NWnPVXo5cW6lcnXhd4V{KE[jczDtVm5CKGyndnXs NW[3TJVmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlE3QTVpPkKyOFYyPjl3PD;hQi=>
MDA-MB-231 NEm2T5VCeG:ydH;zbZMhSXO|YYm= MlLXNVAh|ryP MV:0PEBp MUfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NFewXJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi4O|Y6Pyd-MkG4PFc3QTd:L3G+
MCF-7  M3POTWFxd3C2b4Ppd{BCe3OjeR?= NHvrcpgyOCEQvF2= MnTsOFghcA>? M17GdZJm\HWlZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NV:5bm9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4PFc3QTdpPkKxPFg4Pjl5PD;hQi=>
A375 NH7HS5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUWwMlUh|ryP NILNcm8yNzVxODDk MmTwbY5pcWKrdIOgdJJwdGmoZYLheIlwdiCjbnSgbY5lfWOnczDkbYZn\XKnboTpZZRqd25ib3[gcYVt[W6xbXGgZ4VtdHN? NFW3bHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe5OlYzOid-MkG3PVY3OjJ:L3G+
SKMEL1 M3zZbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljuNE42KM7:TR?= NGfMbXgyNzVxODDk MX\pcohq[mm2czDwdo9tcW[ncnH0bY9vKGGwZDDpcoR2[2W|IHTp[oZmemWwdHnheIlwdiCxZjDt[Yxidm:vYTDj[Yxtew>? NFHHRnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe5OlYzOid-MkG3PVY3OjJ:L3G+
SKMEL3 MlPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2T3dVAvPSEQvF2= NYrTcHVQOS93L{ig[C=> NIm5Z3BqdmirYnn0d{Bxem:uaX\ldoF1cW:wIHHu[EBqdmS3Y3XzJIRq\m[ncnXueIlifGmxbjDv[kBu\Wyjbn;tZUBk\Wyucx?= NITxTpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe5OlYzOid-MkG3PVY3OjJ:L3G+
SKMEL28 Mm\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGO2dY8xNjVizszN M1\nV|EwPS96IHS= NYLrOW9PcW6qaXLpeJMheHKxbHnm[ZJifGmxbjDhcoQhcW6mdXPld{BlcW[oZYLlcpRq[XSrb36gc4YhdWWuYX7vcYEh[2WubIO= M33MNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{m2OlIzLz5{MUe5OlYzOjxxYU6=
MeWo MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\uRnUxNjVizszN MVixM|UwQCCm M{HGTIlvcGmkaYTzJJBzd2yrZnXyZZRqd25iYX7kJIlv\HWlZYOg[Iln\mW{ZX70bYF1cW:wIH;mJI1mdGGwb33hJINmdGy| NEW4VZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe5OlYzOid-MkG3PVY3OjJ:L3G+
B16 M1rDZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHsR2ExNjVizszN NH\zcHAyNzVxODDk MoHwbY5pcWKrdIOgdJJwdGmoZYLheIlwdiCjbnSgbY5lfWOnczDkbYZn\XKnboTpZZRqd25ib3[gcYVt[W6xbXGgZ4VtdHN? MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5OU[2NlIoRjJzN{m2OlIzRC:jPh?=
Ly 1 MmLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PFe|I1KGh? NGTIO2hKSzVyPUeuN{DPxE1? M4\JUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
Ly 7 NFTXemhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3tb5AzPCCq MoDrTWM2OD1zMD63JO69VQ>? NUjlRYZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3O|IxPDlpPkKxO|czODR7PD;hQi=>
Su-DHL6 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Ljb|I1KGh? NYjhdnN2UUN3MP-8olIxKM7:TR?= M1HZTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
Ly 10 NXXHT|RWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHSO2wzPCCq M1PVWGlEPTExvK6yNEDPxE1? M3jqZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
RIVA NGG1bnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV25T4lXOjRiaB?= Mlf3TWM2OO,:nkKwJO69VQ>? NFq5PJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O|IxPDl:L3G+
Su-DHL2 NIHQd4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHJNlQhcA>? M17WTWlEPTExvK6yNEDPxE1? NFr3SFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O|IxPDl:L3G+
Ly 1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4ewNlQ5KGh? M3[wOmlEPTB;MD6zOEDPxE1? M1T4UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
Ly 7 MmnkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFq0cHE1QCCq Mm\3TWM2OD1yLkCyOUDPxE1? NF7VeXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O|IxPDl:L3G+
Su-DHL6 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPodFBqPDhiaB?= NVrSOlIxUUN3MP-8olIxKM7:TR?= NF7mdIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O|IxPDl:L3G+
Ly 10 M3OzSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVu0PEBp MVHJR|UxRTFwODFOwG0> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
RIVA MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTKXJl[PDhiaB?= M{nybGlEPTExvK6yNEDPxE1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
Su-DHL2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfmeYMyPDhiaB?= NXryd4hTUUN3ME2xO{41KM7:TR?= MlO3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5N{KwOFkoRjJzN{eyNFQ6RC:jPh?=
Ly 1 M4\LUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnzUVQ4OiCq NYPKOldXUUN3ME2wMlAyKM7:TR?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
Ly 7 M3TQNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLj[4pqPzJiaB?= MXfJR|UxRTBwMEG4JO69VQ>? M3;VWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
Su-DHL6 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\SdHRQPzJiaB?= NWm2RolJUUN3ME2xMlYh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
Ly 10 M2m4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEPNUlY4OiCq MkXVTWM2OD1zLkKg{txO MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
RIVA Mlf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWe3NkBp NIHjN2hKSzVy78{eNlAh|ryP NHG0d3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O|IxPDl:L3G+
Su-DHL2 NVq0OFA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTrWo5YPzJiaB?= NVn4ZZN2UUN3ME2xNU4zKM7:TR?= NVX0[YNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3O|IxPDlpPkKxO|czODR7PD;hQi=>
U373-MAGI NFS4fYhCdnSrdnnyZYwh[XO|YYm= MWewMlI2KHSxIEigeW0> MVuyJJRwKDd{IHjydy=> NETrfGtCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBXW1ZvRzDwd4V2\G:2eYDl[EBJUVZvMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHU2NWejZzDs[ZZmdCCjdDCwMlI2KHSxIEigeW0h[W[2ZYKgNkB1dyB5MjDodpMh[nlicWDDVkBu\XSqb3S= M3;oVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI MWTBcpRqfmm{YXygZZN{[Xl? MWOwMlI2KHSxIEigeW0> Mmr6NkB1dyB5MjDodpM> NUjieZZwSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXlOYLVegdJNmfWSxdInw[YQhUEmYLUGgUmw1NTNiaX7m[YN1\WRiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBo[WdibHX2[Ywh[XRiMD6yOUB1dyB6IIXNJIFnfGW{IEKgeI8hPzJiaILzJIJ6KHGSQ2KgcYV1cG:m MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Survivin / Bcl-2 / p53 / c-Myc / DNMT1; 

PubMed: 26384351     


HL60/ADR and kasumi-1 cells were lysed after treated with 0, 1, 2, 4μM decitabine for 72h, and antibodies against β-actin was used as loading control of whole cell proteins in western blotting. The expression of DNMT1, c-Myc, P53, Bcl-2 and Survivin were determined in AML cells.

p-AKT / AKT / p-GSK3β / GSK3β / p-Myc / Myc / p-P70 / P70 / p-4EBP-1 / 4EBP-1 / PTEN; 

PubMed: 25762617     


Effects of decitabine on the expression of survival pathway proteins at the indicated concentrations.

phospho-p38 / p38 / phospho-NFκB / NFκB; 

PubMed: 26617834     


Decitabine suppressed the phosphorylation of p38 and NF-κB.

p-JAK1 / JAK1 / p-JAK2 / JAK2 / p-STAT3 / STAT3 ; 

PubMed: 26692933     


DAC inhibits the activity of JAK/STAT3 signaling pathway in HL-60 cells. A. HL-60 cells were exposed to 0.2-1.0 μM DAC for 48 h. Cell lysates were prepared and subjected to western blot assay for p-STAT3 (Tyr705 and Ser727) and total STAT3 protein. DAC treatment led to the dephosphorylation of STAT3 both at Tyr705 and Ser727, with moderate effect on total STAT3 protein. B. DAC also down-regulated the expression of p-JAK1 and p-JAK2 as well as total JAK1 and JAK2 in HL-60 cells. The protein expression of STAT3 and JAKs was normalized to that of β-actin and results are representative from three independent experiments.

E-cadherin / N-cadherin / Snail / MMP-2 / MMP-9 / Bcl-2 / Bax; 

PubMed: 28152502     


The protein level of EMT and invasion related proteins E-cadherin, N-cadherin, Snail, MMP-2, MMP-9 and the protein level of the apoptotic-related proteins, BCL-2 and Bax were detected by western blot. β-actin served as a internal control.

26384351 25762617 26617834 26692933 28152502
Immunofluorescence
DNMT1; 

PubMed: 21303982     


Ren-01 cells (low passage number RCC cells) at <40% confluence were treated with decitabine 0.5 μM. DNMT1 was quantified 48 hours later by immuno-fluorescence (green dots). DAPI was used to stain nuclei (blue stain).

E-cadherin / MMP-9; 

PubMed: 28152502     


Immunofluorescence microscopy to identify invasion-promoting molecule MMP-9 and EMT-protective molecule E-cadherin in YES-2 and TE13 cells treated with DAC. 

21303982 28152502
Growth inhibition assay
Cell viability; 

PubMed: 26384351     


Cells were treated with Decitabine for 72 h. Viability was measured by MTT assay in AML cells and patient samples Different AML cells.

26384351
体内研究 Decitabine 按2.5 mg/kg 剂量作用于ALK+ KARPAS-299小鼠移植瘤模型,促进凋亡,且降低肿瘤细胞增殖,也导致肿瘤抑制基因p16INK4A的去甲基化。 [2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

DNA合成检测 :

通过测定放射性胸甘摄入到DNA的情况而测定DNA合成比率。HL-60 和KG1a 细胞悬浮在6孔(直径为35 mm )盘中,孔中为含10%胎牛血清的2 mL RPMI 培养基中,然后与不同浓度相应药物温育48小时 (药物同时加入)。48小时时, 每孔加入0.5 μCi [3H] 胸甘(6.7 Ci/mmol),再温育24小时。细胞置于GF/C玻璃纤维过滤器(直径为2.4 cm)中,使用冷 0.9% NaCl, 5% 冷三氯乙酸和乙醇清洗。烘干含DNA的过滤器,置于EcoLite闪烁液(ICN)中,使用 Beckman LS 6000IC 闪烁计数器测量放射性。通过剂量-反应曲线计算IC50值。
细胞实验:[1]
- 合并
  • Cell lines: HL-60 和 KG1a
  • Concentrations: 0 到100 ng/mL
  • Incubation Time: 96 小时
  • Method: 生长抑制实验中,指数生长期细胞接种在5 ml培养基中。向培养基中同时加入不同浓度Decitabine。在指定时间,使用模型 ZM Coulter 计数器对细胞进行计数。根据药物处理的白血病细胞的生长曲线,测定IC50值。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: KARPAS-299 人类细胞皮下接种到小鼠的左右两侧。
  • Dosages: ≤2.5 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 45 mg/mL (197.18 mM)
Water 10 mg/mL (43.81 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
water
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 228.21
化学式

C8H12N4O4

CAS号 2353-33-5
储存条件 粉状
溶于溶剂
别名 Deoxycytidine, Dacogen, 5-aza-2'-deoxycytidine, 5-AZA-dC, 5-aza-CdR

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04985656 Not yet recruiting Drug: Pevonedistat|Drug: Decitabine|Drug: Cedazuridine Myelodysplastic Syndromes (MDS) Takeda October 1 2021 Phase 2
NCT04854889 Recruiting Drug: Decitabine Refractory Aplastic Anemia Institute of Hematology & Blood Diseases Hospital April 22 2021 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is S1200 a racemic mixture or a monomer?

  • 回答:

    S1200 is R form

DNA Methyltransferase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID