Dexamethasone (MK-125)

For research use only. Not for use in humans.

目录号:S1322 别名: NSC 34521, Hexadecadrol, Prednisolone F 中文名称:地塞米松

Dexamethasone (MK-125) Chemical Structure

CAS No. 50-02-2

Dexamethasone (MK-125, NSC 34521, Hexadecadrol, Prednisolone F)是一种有效的半合成的糖皮质激素类甾体药物,也是一种interleukin receptor调节剂,具有抗炎和免疫抑制作用。Dexamethasone 可诱导自噬和线粒体自噬。Dexamethasone 被用于COVID-19临床病人测试中,并发现对危重症患者有一定效果。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1061.25 现货
RMB 785.41 现货
RMB 2211.3 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Dexamethasone (MK-125)发表文献52篇:

产品安全说明书

IL Receptor抑制剂选择性比较

生物活性

产品描述 Dexamethasone (MK-125, NSC 34521, Hexadecadrol, Prednisolone F)是一种有效的半合成的糖皮质激素类甾体药物,也是一种interleukin receptor调节剂,具有抗炎和免疫抑制作用。Dexamethasone 可诱导自噬和线粒体自噬。Dexamethasone 被用于COVID-19临床病人测试中,并发现对危重症患者有一定效果。
靶点
interleukin receptor [1]
(Cell-free assay)
体外研究

在永生化鼠脑内皮细胞株(GPNT)中,Dexamethasone导致了单层细胞对蔗糖,荧光素和20 KDa葡聚糖通透性的降低。在培养的大鼠脑血管内皮细胞的细胞质中,Dexamethasone导致丝状肌动蛋白和相关的细胞骨架蛋白皮层蛋白被高度集中在很少的F-肌动蛋白应力纤维可见的细胞和细胞接触的区域,这和Dexamethasone诱导的分化障碍表型一致。Dexamethasone强烈刺激的Id-1蛋白的表达,它是血清诱导的螺旋-环-螺旋转录抑制子,参与细胞分化的水平,并且这种作用被证明是在CON8乳腺上皮肿瘤细胞的细胞周与ZO-1的重组相关联。Dexamethasone可防止细胞因子诱导MMP-9表达的增强,并在未处理GPNT单层细胞中抑制ZO-1表达。 [1] 在肺泡上皮细胞中,Dexamethasone通过下调伽玛GCS重亚单位的转录消耗基础和肿瘤坏死因子-α刺激的谷胱甘肽水平下调,通过AP-1(c-Jun)途径。在肺泡上皮细胞(A549)中,Dexamethasone降低基础和刺激的GSH水平(TNF-α处理的),而不对GSSG有任何变化。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human monocytes MmnySpVv[3Srb36gZZN{[Xl? MVy1JO69VQ>? M1TYblIxKGh? M4n4bWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hcHWvYX6gcY9vd2O7dHXzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhS0:[MjDlfJBz\XO|aX;uJIF1KDVidV2gZYZ1\XJiMkCgbJJ{KHW|aX7nJJJi[mKrdDDwc4x6[2yxbnHsJIFvfGmkb3T5JGloTyCkeTDTSHMuWEGJRTDhcoFtgXOrcx?= Mni4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NEO5N|UoRjJzOESzPVM2RC:jPh?=
rat polymorphonuclear leukocytes M2rLbmZ2dmO2aX;uJIF{e2G7 MUOyNEBucW6| M3T5cGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hemG2IIDvcJlud3KyaH;ueYNt\WG{IHzleYtw[3m2ZYOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMue3SrbYXsZZRm\CCwaYTybYMhd3irZHWgdJJw\HWldHnvckBi\nSncjCyNEBucW6|IHL5JGdzcWW|czDt[ZRpd2RuIFnDOVA:Oi5|IH7N NHPOSXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWwOlYzOCd-MkK1NFY3OjB:L3G+
mouse thymocytes MULGeY5kfGmxbjDhd5NigQ>? NHnWcWcyKM7:TR?= NV3RVWFIOjRiaB?= MkfSTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDtc5V{\SC2aIntc4N6fGW|IHHzd4V{e2WmIHHzJIlvfGW{boXjcIVwe2:vYXygSG5CKG[{YXft[Y51[XSrb36gZZQhOSC3TTDh[pRmeiB{NDDodpMh[nliYXfhdo9{\SCpZXyg[Yxm[3S{b4Doc5Jme2m| M17RdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{m0O|Q5Lz5{NEe5OFc1QDxxYU6=
T-cell NEHPPZpHfW6ldHnvckBie3OjeR?= M4O2fmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IGSgZ4VtdCCycn;sbYZmemG2aX;uJJV{cW6pIHPvcoNidmG4YXzpckBCKHO2aX31cIF1\WRibXnj[UB{eGynZX6gZ4VtdHNuIFnDOVAhRSByLkCwOUDPxE1w M4\2PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMkK5O|Y4Lz5zMUKyPVc3PzxxYU6=
PBMC Mn7DSpVv[3Srb36gZZN{[Xl? NILNcINKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDUUmYu[WyyaHGgdJJw\HWldHnvckBnem:vIHj1cYFvKHCncnnwbIVz[WxiYnzvc4QhdW:wb371Z4xm[XJiY3XscJMhe3SrbYXsZZRm\CCkeTDMVHMtKEmFNUCgQUAxNjBzIN88UU4> MofOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{Mkm3OlcoRjFzMkK5O|Y4RC:jPh?=
PBMC M2PU[WZ2dmO2aX;uJIF{e2G7 MYjJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCpcn;3eIghd2ZiTGDTJJN1cW23bHH0[YQhcHWvYX6gdIVzcXCqZYLhcEBjdG:xZDDtc45wdnWlbHXhdkBk\WyuczygTWM2OCB;IECuNFQh|ryPLh?= MmLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{Mkm3OlcoRjFzMkK5O|Y4RC:jPh?=
RAW264.7 NU\HNHdtSW62aXnu[oxidW2jdH;yfUBie3OjeR?= NHvrcHUzPCCqcoO= M1i4SmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiZX\m[YN1KG:wIGTOSoFteGijIH\vdo1ifGmxbjDwdoV1emWjdHXkJFEhcHJiYnXmc5JmKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{NCCLQ{WwJF0hOC55IN88UU4> M1fhRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOUC4PVg1Lz5zMUmwPFk5PDxxYU6=
N9 MUHGeY5kfGmxbjDhd5NigQ>? M3HsOmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBVVkZvYXzwbIEhemWuZXHz[UBqdiCQOTDj[YxteyCrbjD0bIUhe3WyZYLuZZRidnRuIFnDOVAhRSByLkC3OEDPxE1w MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjN4MUO5OUc,OTJ|NkGzPVU9N2F-
RAW 264.7 MX3GeY5kfGmxbjDhd5NigQ>? NGfMVWpKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhXE6ILXHsdIhiKHKnbHXhd4UhcW5iUlHXJFI3PC55IHPlcIx{KGmwIITo[UB{fXCncn7heIFvfCxiSVO1NEA:KDBwNEKg{txONg>? NIXqR5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkO2NVM6PSd-MUKzOlE{QTV:L3G+
A549 M2\YWWZ2dmO2aX;uJIF{e2G7 NHXqT|IyOCC3TR?= MYLBcpRi\2:waYP0JIFkfGm4aYT5JIZweiCJbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCPTWTWJJRz[W6|YXP0bZZifGmxbjDhd5NigSCrbjDoeY1idiCDNUS5JIx2dmdiZYDpeIhmdGmjbDDj[YxteyCjdDCxNEB2VSxiSVO1NEA:KDFyIN88UU4> NFuyXo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm5PVk5QSd-MUW5PVk6QDl:L3G+
A549 NG\xZVRHfW6ldHnvckBie3OjeR?= M4fJe2FkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiZX\m[YN1KG:wIFfSSUBxem:vb4TldkBz\XOyb37z[UBjgSCudXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMEGg{txONg>? M2\Ue|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkSzNFYxLz5zNk[0N|A3ODxxYU6=
A549 NGr0flVHfW6ldHnvckBie3OjeR?= MknzRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWiCjc4Pld5Nm\CCjczDNUXRXNW2nZHnheIVlKHS{YX7zZYN1cX[jdHnvckBw\iC{ZX7pcIxiKGy3Y3nm[ZJie2ViZ3Xu[UBqdiCqdX3hckBCPTR7IHPlcIx{KHKnbHH0bZZmKHSxIFTlfIFu\XSqYYPvcoUtKEWFNUCgQUAxNjByNUCxNkDPxE1w Mk\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ6MkG3PFEoRjF4OEKxO|gyRC:jPh?=
A549 M3XiWmZ2dmO2aX;uJIF{e2G7 MXrB[49vcXO2IHHjeIl3cXS7IHH0JGdTKGG|c3Xzd4VlKGG|IF7GMYtieHCjQj3t[YRq[XSnZDD0doFve3KncILld5Nqd25ib3[gd4VkemW2ZXSgdIxi[2WwdHHsJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGenbnWgbY4hcHWvYX6gRVU1QSClZXzsd{whUUN3MDC9JFAvODBzMUe1JO69VS5? NXviSXRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4QDFpPkG2PFIyPzhzPD;hQi=>
HFF MoTjSpVv[3Srb36gZZN{[Xl? MV3JcoR2[3Srb36gc4Yh[XKxbXH0ZZNmKGGldHn2bZR6KGmwIFjGSkBk\WyuczDhd5Nme3OnZDDhd{Bxem:mdXP0bY9vKG:oIHLleIEh\XO2cnHkbY9tKGK7IHHyc41ifGG|ZTDhd5NigSxiRVO1NEA:KDBwMECxPUDPxE1w MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF6MUG3Nkc,OTdzOEGxO|I9N2F-
HeLa NYmzd4k4TnWwY4Tpc44h[XO|YYm= NILjOmJC[3SrdnH0bY9vKG:oIF3NWHYhcW5iSHXMZUBk\WyuczDt[YF{fXKnZDDifUBtfWOrZnXyZZNmKGGldHn2bZR6NCCHQ{WwJF0hOC5yMUeg{txONg>? NWDBXm1PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexPFEyPzJpPkG3NVgyOTd{PD;hQi=>
HFF NHW5XZJHfW6ldHnvckBie3OjeR?= MWXJcohq[mm2aX;uJI9nKEmOMT3zeIlufWyjdHXkJGlNPiCycn;keYN1cW:wIHnuJGhHTiClZXzsd{whUUN3MDC9JFAvODByNUGg{txONg>? NYS5fmtHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexPFEyPzJpPkG3NVgyOTd{PD;hQi=>
RAW 264.7 MVjGeY5kfGmxbjDhd5NigQ>? MVeyOEBpenN? M3i2NmlvcGmkaYTpc44hd2ZiaX70[ZJn\XKxbjDnZY1u[S2|dHnteYxifGWmIF7PJJBzd2S3Y4Tpc44hcW5iUlHXJFI3PC55IHPlcIx{KGGodHXyJFI1KGi{czygTWM2OCB;IECuNFIh|ryPLh?= M1fCV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{[3NVI1Lz5zN{O2O|EzPDxxYU6=
RAW264.7 NWPs[Wp[TnWwY4Tpc44h[XO|YYm= NXnYVGdtOiCqcoO= NWD6bWtNUW6qaXLpeIlwdiCxZjDDU3gzKGW6cILld5Nqd25iaX6gUHBUNXO2aX31cIF1\WRibX;1d4UhWkGZMk[0Mlch[2WubIOgZYZ1\XJiMjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyN{K3O{c,OTd2MEeyO|c9N2F-
RAW264.7 MkHqSpVv[3Srb36gZZN{[Xl? NXroPFRsOiCqcoO= MnXoTY5pcWKrdHnvckBw\iCvUFfFV|Eh\XiycnXzd4lwdiCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDh[pRmeiB{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= Mk\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MEeyO|coRjF5NEC3Nlc4RC:jPh?=
A549 M3nqXGZ2dmO2aX;uJIF{e2G7 MoW2RYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDBOVQ6KGOnbHzzJIJ6KE2PVG[geJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEWzO{DPxE1w Mm\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MU[zPVUoRjF5NkG2N|k2RC:jPh?=
A549 NXXQOHJUTnWwY4Tpc44h[XO|YYm= NHfRVGlC\2:waYP0JIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGE2PDliY3XscJMh[nliTl[tb4FxeGGEIITyZY5{emWycnXzd4lwdiCjc4PhfUwhUUN3MDC9JFAvODBzIN88UU4> M4T4UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkG2N|k2Lz5zN{[xOlM6PTxxYU6=
A549 MYPGeY5kfGmxbjDhd5NigQ>? Mmm3RY51[WexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCqdX3hckBCPTR7IHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghVU2WVjDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCmZYjhcYV1cGG|b37lMYlv\HWlZXSgZYN1cX[jdHnvckwhUUN3MDC9JFEh|ryPLh?= NXP2UnpjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NVY{QTVpPkG3OlE3Ozl3PD;hQi=>
HFF NHSzcY1CdnSraX7mcIFudWG2b4L5JIF{e2G7 NEX4OFFCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gTWwyKHO2aX31cIF1\WRiSVy2JJBzd2S3Y4Tpc44hcW5iSF\GJINmdGy|IHL5JJRz[W6|cnXwdoV{e2mxbjDhd5NigSxiRVO1NEA:KDBwMECwOUDPxE1w NIfERno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NlUyQSd-MUe2PVI2OTl:L3G+
HFF MUHBcpRqcW6obHHtcYF1d3K7IHHzd4F6 NV3PNoY3SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4Yh[XKxbXH0ZZNmKGmwIFjGSkBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCwNkDPxE1w M3HOdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkmyOVE6Lz5zN{[5NlUyQTxxYU6=
CV1 M3XxWWZ2dmO2aX;uJIF{e2G7 Mk\KRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHVkBmgHC{ZYPz[YQhcW5iQ2[xJINmdGy|IHL5JGdTTSCjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMECyJO69VS5? NWL5dmdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NFU{PjJpPkG3O|A2OzZ{PD;hQi=>
NHDFneo M4fDOWZ2dmO2aX;uJIF{e2G7 MVHBZ5Rqfmm2eTDheEBpfW2jbjDHVkBmgHC{ZYPz[YQhcW5iTljESo5mdyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGlNNTZicILv[JVkfGmxbjygTWM2OCB;IECuNFAyPCEQvF2u MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdyNUO2Nkc,OTd5MEWzOlI9N2F-
RPMI8226 Mm\zRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NUXxWIszPCCmYYnz NYfXeVk6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDSVG1KQDJ{NjDj[YxteyCjZoTldkA1KGSjeYOsJGlEPTBiPTCwMlAxPjVizszNMi=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdyNUO2Nkc,OTd5MEWzOlI9N2F-
J774A.1 NHT6W|JHfW6ldHnvckBie3OjeR?= M1vWb2lvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5H[WyyaHGgdJJw\HWldHnvckBqdiCvb4Xz[UBLPzd2QT6xJINmdGy|LDDJR|UxKD1iMD6wOFgh|ryPLh?= M4XLbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEmyPVQyLz5zN{i5Nlk1OTxxYU6=
CV1 MmGySpVv[3Srb36gZZN{[Xl? MX3BZ5Rqfmm2eTDheEBpfW2jbjDncJVkd2OxcoTpZ49q\CC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR3YyKGOnbHzzJIF{e2W|c3XkJIF{KHKncILld5Nqd25ib3[gWG5HKGGwZDDJUE0yNWKndHGtbY5lfWOnZDDFMZNmdGWldHnuJIdmdmViZYjwdoV{e2mxbjDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXEOVAhRSByLkCwNFEh|ryPLh?= MnO0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyM{K2NVAoRjF6MEOyOlExRC:jPh?=
A549 NYT6R2ZsTnWwY4Tpc44h[XO|YYm= NYL1OGVJSWexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCqdX3hckBCPTR7IHPlcIx{KGK7IF3NWHYhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNFUxOTJizszNMi=> MmLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyM{i5O|AoRjF6MEO4PVcxRC:jPh?=
A549 NYTJSIhtTnWwY4Tpc44h[XO|YYm= MmDWRYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDBOVQ6KGOnbHzzJIJ6KE6ILXvhdJBiSiC2cnHud5JmeHKnc4Ppc44h[XO|YYmsJGlEPTBiPTCwMlAxOTF5NTFOwG0v NF;SNGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECzPFk4OCd-MUiwN|g6PzB:L3G+
COS7 MknDSpVv[3Srb36gZZN{[Xl? MYexJJVO NXzpOWVkSWexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBSPTdyQTDteZRidnRiaX6gR29UPyClZXzsd{BifCBzIIXNJIJ6KE6ILXvhdJBiSi2udXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= NVvlZ5puRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwN|g6PzBpPkG4NFM5QTdyPD;hQi=>
HeLa MWHDfZRwfG:6aXPpeJkh[XO|YYm= M4jtO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBFVkFicnXwcIlk[XSrb36gZpkhcW2jZ3nu[{BidmGueYPpdy=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB4NkC1OUc,OThyNk[wOVU9N2F-
HeLa MljySpVv[3Srb36gZZN{[Xl? MkTnTY5pcWKrdHnvckBw\iCvaYTvd4l{KGmwIHj1cYFvKEinTHGgZ4VtdHNiYomgbY1i\2mwZzDhcoFtgXOrcx?= NFGxSIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC2OlA2PSd-MUiwOlYxPTV:L3G+
R1 Ml3XSpVv[3Srb36gZZN{[Xl? NXzMSZR5OTBidV2= NUG5[plzOjRiaILz MlnWTY5pcWKrdHnvckBw\iCpbIXjc4NwenSrY3;p[EBz\WOncITvdk1odHWlb3PvdpRq[2:rZDDjc41xdGW6IITyZY5{\mWldHXkJIlvKHKjdDDSNUBk\WyuczDhd5Nme3OnZDDhd{Bld3ewLYLl[5Vt[XSrb36gc4YhXFCDLYLld5BwdnOrdnWg[YxmdWWwdDDwdo9qdm[uYX3tZZRwenliZ3Xu[UBmgHC{ZYPzbY9vKGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBj\XSjLXfhcIFkfG:|aXThd4Uh[XO|YYmsJGlEPTBiPTCwMlAxPSEQvF2u MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDRzMkO5O{c,OTh2MUKzPVc9N2F-
THP1 MkG4RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NW\PfmM2SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiSVy2JIJ6KEWOSWPBMEBKSzVyIE2gNE4xODdizszNMi=> MoHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW1OlAoRjF6NkK1OVYxRC:jPh?=
THP1 MX7BcpRqcW6obHHtcYF1d3K7IHHzd4F6 MVnBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIGTIVFEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBVVk[jbIDoZUBjgSCHTFnTRUwhUUN3MDC9JFAvODVizszNMi=> NIHp[nc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVU3OCd-MUi2NlU2PjB:L3G+
RAW264.7 MWTGeY5kfGmxbjDhd5NigQ>? M1y5eVExKHWP MoHsTY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBEV1h{IHX4dJJme3Orb36gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYSgNVAhfU1iYomgW4V{fGW{bjDicI91 Mn:4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5OUC2OFkoRjF6N{mwOlQ6RC:jPh?=
RAW264.7 MWjGeY5kfGmxbjDhd5NigQ>? NYTY[XVPUW6qaXLpeIlwdiCxZjDMVHMue3SrbYXsZZRm\CCvaXPyc5NwdWGuIIDyc5N1[WeuYX7kbY4hTSC|eX70bIF{\SBzIHX4dJJme3Orb36gZZQhOTBidV2gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYomgW4V{fGW{bjDicI91 MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd7ME[0PUc,OTh5OUC2OFk9N2F-
A549 NF;QPW5HfW6ldHnvckBie3OjeR?= NGLVPJpVemGwc4LldJJme3Orb36gZYN1cX[rdImgZZQhT1JiaX6gTWwuOS2kZYThMZN1cW23bHH0[YQhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJG5HNWujcIDhRk1l\XCnbnTlcpQhTS2|ZXzlZ5RqdiC2cnHud4NzcXC2aX;uJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAyOSEQvF2u M{O3flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{KxN|QyLz5zOUOyNVM1OTxxYU6=
A549 NXrabXhYTnWwY4Tpc44h[XO|YYm= MkjtWJJidnO{ZYDy[ZN{cW:wIHHjeIl3cXS7IHH0JGdTKGmwIGDNRU1{fGmvdXzheIVlKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCWDFicnXzdI9ve2ViZXzlcYVvfC2rbnT1Z4VlKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBi[3Srdnn0fUwhTUN3MDC9JFAvODB{NTFOwG0v M4XvT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{KxN|QyLz5zOUOyNVM1OTxxYU6=
NP1 MWPGeY5kfGmxbjDhd5NigQ>? NHjhNnJC\2:waYP0JIFkfGm4aYT5JIF1KEeUIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iaIXtZY4hVlBzIHPlcIx{KGG|c3Xzd4VlKGG|IHfseYNw[2:{dHnjc4llKHKnc4DvcpNmKGWuZX3lcpQhfHKjboPhZ5RqfmG2aX;uJIJ6KEeDTESgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMESyJO69VS5? Mn;xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|MkGzOFEoRjF7M{KxN|QyRC:jPh?=
TH2 NXzic2hjTnWwY4Tpc44h[XO|YYm= MlHqOFghcHK| MlTpTY5pcWKrdHnvckBw\iCycn:tbY5ndGGvbXH0c5J6KGO7dH;rbY5mKEmONTDwdo9lfWO2aX;uJIlvKG:4YXzieY1qdi2|dHnteYxifGWmIFLBUGIw[yCvb4Xz[UBVUDJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJNidmS5aXPoMWVNUVODLDDJR|UxKD1iMD6wNFUh|ryPLh?= NGXIO4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOyN|Q5Oyd-MUmzNlM1QDN:L3G+
TH2 NHPzOGhHfW6ldHnvckBie3OjeR?= MYW0PEBpenN? NVzIO5pRUW6qaXLpeIlwdiCxZjDwdo8ucW6obHHtcYF1d3K7IHP5eI9scW6nIFnMNVMheHKxZIXjeIlwdiCrbjDveoFt[nWvaX6td5RqdXWuYYTl[EBDSUyEL3OgcY92e2ViVFiyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSC|YX7ke4lkcC2HTFnTRUwhUUN3MDC9JFAvODB3IN88UU4> MmDNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|MkO0PFMoRjF7M{KzOFg{RC:jPh?=
MDA-kb2 MXvGeY5kfGmxbjDhd5NigQ>? NUDmSWxsSWexbnnzeEBi[3Srdnn0fUBifCCjbnTyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCPRFGtb4IzKGOnbHzzJIF{e2W|c3XkJIF{KHO2aX31cIF1cW:wIH;mJIx2[2moZYLhd4Uh[WO2aY\peJkh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzF3MDC9JFAvOTl2IN88UU4> M2jQc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUmyNlQ2Lz5zOUW5NlI1PTxxYU6=
MDA-kb2 NI\tSmxHfW6ldHnvckBie3OjeR?= M4nmZWFod26rc4SgZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gUWRCNWukMjDj[YxteyCjc4Pld5Nm\CCjczDzeIlufWyjdHnvckBw\iCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVOxOVAhRSByLkG5OEDPxE1w MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV7MkK0OUc,OTl3OUKyOFU9N2F-
A549 NGLES5RHfW6ldHnvckBie3OjeR?= NFyxZohC\2:waYP0JIFkfGm4aYT5JIF1KEeUIHnuJIh2dWGwIFG1OFkh[2WubIOgZpkhVkZva3HwdIFDKHS{YX7zdoVxemW|c3nvckBie3OjeTygTWM2OCB;IECuNFAyKM7:TT6= NF7yTpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW5NlI1Pyd-MUm1PVIzPDd:L3G+
COS7 NYrTcmhkTnWwY4Tpc44h[XO|YYm= MXHB[49vcXO2IHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStUGJFKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODB5MjFOwG0v M{L3blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OE[zNFg{Lz5zOUi2N|A5OzxxYU6=
A549 MXjGeY5kfGmxbjDhd5NigQ>? MVG2JIhzew>? NF7r[lVVemGwc4LldJJme3Orb36gZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBKVC1zLXLleIEucW6mdXPl[EBPTi2tYYDwZWIh\GWyZX7k[Y51KEVvc3Xs[YN1cW:wIIDyc41wfGW{IHHjeIl3[XSrb36gZYZ1\XJiNjDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJGVNSU1iYYPzZZktKEWFNUCgQUAxNjByMUGg{txONg>? NYjYSldGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwOFczQDBpPkKwNFQ4OjhyPD;hQi=>
A549 NYrjeWdWTnWwY4Tpc44h[XO|YYm= NHzVe5U3KGi{cx?= NYPDdIhEXHKjboPy[ZBz\XO|aX;uJIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhWE2DLXnu[JVk\WRiQWCxJIFkfGm4aYT5JIFnfGW{IE[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODB{NTFOwG0v MknIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNEeyPFAoRjJyMES3NlgxRC:jPh?=
HeLa M1jtTGZ2dmO2aX;uJIF{e2G7 MlPkWJJidnOjY4TpeoF1cW:wIH;mJGdCVC12IITh[4dm\CCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhVlBzIHHjeIl3[XSrb36gZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNFQ2KM7:TT6= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB2N{K4NEc,OjByNEeyPFA9N2F-
NIH-3T3 Mlu4SpVv[3Srb36gZZN{[Xl? NY\PbIY6OC5yMTDt[{9s\w>? MWexJIhz NIm0WXZRdGG|bXGgZ49v[2WwdILheIlwdiCrbjDtc5V{\SCkZXHybY5oKG2xdYPlJG5KUC1|VEOgZ4VtdHNiaX70[Ydz[XSnZDD3bZRpKE2PVG[gdoVxd3K2ZYKg[4Vv\SCjdDCwMlAyKG2pL3vnMEBxdyCjZoTldkAyKGi{LDDDdEA:KDBwMES0JO69VS5? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR4OUi2PEc,OjB2Nkm4Olg9N2F-
NIH-3T3 NVLESFVXTnWwY4Tpc44h[XO|YYm= MnfSNE4yKG2pL3vn M3\DOVEhcHJ? MkHnVIxie22jIHPvcoNmdnS{YYTpc44hcW5ibX;1d4Uh[mWjcnnu[{Bud3W|ZTDOTWguO1R|IHPlcIx{KGmwdHXndoF1\WRid3n0bEBOVVSYIILldI9zfGW{IHflcoUh[XRiMD6xJI1oN2upLDDwc{Bi\nSncjCxJIhzNCCFcDC9JFAvOjN4IN88UU4> NVfQXGdyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0Olk5PjhpPkKwOFY6QDZ6PD;hQi=>
NIH-3T3 NEf5bmlHfW6ldHnvckBie3OjeR?= NIHWXHoyKG2pL3vn Ml\1NUBpeg>? MnvXVIxie22jIHPvcoNmdnS{YYTpc44hcW5ibX;1d4Uh[mWjcnnu[{Bud3W|ZTDOTWguO1R|IHPlcIx{KGmwdHXndoF1\WRid3n0bEBOVVSYIILldI9zfGW{IHflcoUh[XRiMTDt[{9s\yxicH:gZYZ1\XJiMTDodkwhS3BiPTCxMlgh|ryPLh?= NHPnO2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES2PVg3QCd-MkC0Olk5Pjh:L3G+
NIH-3T3 M3\DVWZ2dmO2aX;uJIF{e2G7 MlzINVAhdWdxa3e= NV\CZph1OSCqch?= M2rtXXBt[XOvYTDjc45k\W62cnH0bY9vKGmwIH3veZNmKGKnYYLpcochdW:3c3WgUmlJNTOWMzDj[YxteyCrboTl[5JifGWmIIfpeIghVU2WVjDy[ZBwenSncjDn[Y5mKGG2IEGwJI1oN2upLDDwc{Bi\nSncjCxJIhzNCCFcDC9JFE4NjdizszNMi=> M3P3dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNE[5PFY5Lz5{MES2PVg3QDxxYU6=
NIH-3T3 Mn\iSpVv[3Srb36gZZN{[Xl? NFfhfooxNjBzIH3nM4to NVvlenVoOSCqch?= MX\GdoFkfGmxbjD1coJwfW6mIHnuJI1wfXOnIIDsZZNu[SCkZXHybY5oKG2xdYPlJG5KUC1|VEOgZ4VtdHNiaX70[Ydz[XSnZDD3bZRpKE2PVG[gdoVxd3K2ZYKg[4Vv\SCjdDCwMlAyKG2pL3vnMEBxdyCjZoTldkAyKGi{LDDGeUA:KDBwMECyNFMh|ryPLh?= NIXzOnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES2PVg3QCd-MkC0Olk5Pjh:L3G+
NIH-3T3 MXXGeY5kfGmxbjDhd5NigQ>? M1nwN|AvOSCvZz;r[y=> MXOxJIhz NV;ae4xPTnKjY4Tpc44hfW6kb4Xu[EBqdiCvb4Xz[UBxdGG|bXGgZoViemmwZzDtc5V{\SCQSVitN3Q{KGOnbHzzJIlvfGWpcnH0[YQhf2m2aDDNUXRXKHKncH;yeIVzKGenbnWgZZQhOC5zIH3nM4toNCCybzDh[pRmeiBzIHjyMEBHfSB;IECuNFEyPiEQvF2u M4S3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNE[5PFY5Lz5{MES2PVg3QDxxYU6=
NIH-3T3 MnjrSpVv[3Srb36gZZN{[Xl? NIXKN2MyKG2pL3vn NFrNZlYyKGi{ MWrGdoFkfGmxbjD1coJwfW6mIHnuJI1wfXOnIIDsZZNu[SCkZXHybY5oKG2xdYPlJG5KUC1|VEOgZ4VtdHNiaX70[Ydz[XSnZDD3bZRpKE2PVG[gdoVxd3K2ZYKg[4Vv\SCjdDCxJI1oN2upLDDwc{Bi\nSncjCxJIhzNCCIdTC9JFAvODh5IN88UU4> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR4OUi2PEc,OjB2Nkm4Olg9N2F-
NIH-3T3 M3fKN2Z2dmO2aX;uJIF{e2G7 MkjINVAhdWdxa3e= MmXSNUBpeg>? MUXGdoFkfGmxbjD1coJwfW6mIHnuJI1wfXOnIIDsZZNu[SCkZXHybY5oKG2xdYPlJG5KUC1|VEOgZ4VtdHNiaX70[Ydz[XSnZDD3bZRpKE2PVG[gdoVxd3K2ZYKg[4Vv\SCjdDCxNEBu\y:tZzygdI8h[W[2ZYKgNUBpeixiRoWgQUA2NjF{MzFOwG0v NUTUWZMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0Olk5PjhpPkKwOFY6QDZ6PD;hQi=>
HeLa Mn\WSpVv[3Srb36gZZN{[Xl? M1fzSXRz[W6|YXP0bZZifGmxbjDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDNUXRXKHC{b33veIVzKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODB5OUSzJO69VS5? M2jn[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyN{K3O|Q{Lz5{MEeyO|c1OzxxYU6=
HeLa M4PmfGZ2dmO2aX;uJIF{e2G7 NXLmeYt3XHKjboPy[ZBz\XO|aX;uJIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yMY1m\GmjdHXkJIFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHRRSS2rbnT1Z4VlKGOxbHzh[4Vv[XOnIIDyc41wfGW{IHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMECxOVg2KM7:TT6= M1zMXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyN{K3O|Q{Lz5{MEeyO|c1OzxxYU6=
THP1 NEX3WGFHfW6ldHnvckBie3OjeR?= MYDUdoFve3KncILld5Nqd25iYXP0bZZqfHliYYSg[4x2[2:lb4L0bYNwcWRicnXj[ZB1d3JvbXXkbYF1\WRiYX70bYlv\myjbX3heI9zgSCjY4Tpeol1gSCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgTWwuQCCycn;keYN1cW:wLDDJR|UxKD1iMD6wNFI2OTJizszNMi=> NIXOcXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEeyO|c1Oyd-MkC3Nlc4PDN:L3G+
Sf9 NXixT4JpTnWwY4Tpc44h[XO|YYm= MonpRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjDy[YNwdWKrbnHueEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiU3[5JINmdGy|LDDJR|UxKD1iMD6wNVI2QSEQvF2u NH;kPZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEeyO|c1Oyd-MkC3Nlc4PDN:L3G+
insect cells MmjOSpVv[3Srb36gZZN{[Xl? MX3CbY5lcW6pIHHm[olvcXS7IITvJIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdidHX0doFu\XSqeXzybI9l[W2rbnWgcIFj\WynZDDE[ZhidWW2aHHzc45mKGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25ibXnjdo9xdGG2ZTDhd5NigSxiSVO1NEA:KDBwMECzOEDPxE1w NFz5cYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEezOVAxOSd-MkC3N|UxODF:L3G+
insect cells NH;tZY9HfW6ldHnvckBie3OjeR?= MULCbY5lcW6pIHHm[olvcXS7IITvJI1qdmW{YXzvZ49zfGmlb3nkJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDpcpNm[3RiY3XscJMhfXOrbnegeIV1emGvZYTofYxzcG:mYX3pcoUhdGGkZXzl[EBF\XijbXX0bIF{d26nIHL5JIZtfW:{ZYPj[Y5k\SCyb3zhdol7[XSrb36gcYlkem:ybHH0[UBie3OjeTygTWM2OCB;IECuNFM{KM7:TT6= NXHITW9yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC3N|UxODFpPkKwO|M2ODBzPD;hQi=>
U2OS MnfISpVv[3Srb36gZZN{[Xl? MVOxPEBpenN? MUnB[49vcXO2IHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{LVzCSEBqdiCqdX3hckBWOk:VIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghT2GuND3ERmQh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[geJJidnOjY4TpeoF1cW:wIHHjeIl3cXS7IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCxOU45PDh7IN88UU4> NHvGWHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEixNlY5OSd-MkC4NVI3QDF:L3G+
U2OS NXTSeHk{TnWwY4Tpc44h[XO|YYm= MX:xPEBpenN? NYCxR2FpSW62YXfvcol{fCCjY4Tpeol1gSCjdDDncJVkd2OxcoTpZ49q\CC{ZXPldJRwei2OQlSgbY4hcHWvYX6gWVJQWyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJGdidDRvRFLEJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geJJidnOjY4TpeoF1cW:wIHHjeIl3cXS7IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlAxODVyOEKg{txONg>? NXTBdIxQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4NVI3QDFpPkKwPFEzPjhzPD;hQi=>
HepG2 NUH6OGc{TnWwY4Tpc44h[XO|YYm= NIDqZlgzPCCqcoO= MlnXRYN1cX[jdHnvckBw\iC{YYSgVHhTKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJIx2dWmwZYPj[Y51KGGwYXz5d4l{NCCHQ{WwJF0hOi54IN88UU4> M4K5OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOU[2NFQ{Lz5{MEm2OlA1OzxxYU6=
A549 M3HGRWZ2dmO2aX;uJIF{e2G7 NHLyT4hVemGwc4LldJJme3Orb36gZYN1cX[rdImgc4Yh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGlNOWKndHGtd5RqdXWuYYTl[EBPTmujcIDhRk1l\XCnbnTlcpQhTS2|ZXzlZ5RqdiC2cnHud4NzcXC2aX;uJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAyOSEQvF2u MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB5M{G5NEc,OjFyN{OxPVA9N2F-
A549 M3\D[2Z2dmO2aX;uJIF{e2G7 NIrvTJRVemGwc4LldJJme3Orb36gZYN1cX[rdImgc4Yh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gRVU1QSClZXzsd{BmgHC{ZYPzbY5oKEGSLUGgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDQUWEucW6mdXPl[EBCWC1zIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECyOUDPxE1w NXfrV5N[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwO|MyQTBpPkKxNFc{OTlyPD;hQi=>
NP-1 Hela MY\GeY5kfGmxbjDhd5NigQ>? NUPHbIRIXHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JI9nKGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gbJVu[W5iTmCtNUBJ\WyjIHPlcIx{KGOxLXX4dJJme3OrbnegS2FNPCCGTlGgZolv\GmwZzDkc41icW5iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMESyJO69VS5? Mkn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyN{OxPVAoRjJzMEezNVkxRC:jPh?=
13D3/Huh7 NHftOWxHfW6ldHnvckBie3OjeR?= MUiyOEBpenN? NV24N3NLXHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJFE{TDNxSIXoO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZidInyc5NqdmViYX3pco91emGwc3\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOsJGVEPTBiPTCwMlAxPSEQvF2u NEnscpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3N|E6OCd-MkGwO|MyQTB:L3G+
A549 M1H6bWZ2dmO2aX;uJIF{e2G7 NUfXbnVTSWexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBqdiCqdX3hckBCPTR7IHPlcIx{KGK7IF7GMYtieHCjQjD0doFve3KncILld5Nqd25iYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v NWTxWZZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVc{ODlpPkKxNlU4OzB7PD;hQi=>
A549 Mlf4SpVv[3Srb36gZZN{[Xl? MnLVRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWiCrbjDoeY1idiCDNUS5JINmdGy|IITyZY5{\mWldHXkJJdqfGhibIXjbYZmemG|ZTDn[Y5mKGyrbnvl[EB1dyCPTWTWJJBzd22xdHXyJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2VidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6NCCLQ{WwJF0hOC5yMEO5PFEh|ryPLh?= MlfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{NUezNFkoRjJzMkW3N|A6RC:jPh?=
RAW264.7 M3OyS2FvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MV[xPEBpenN? M4TmNWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiC2cnXheIVlKDJiaILzJIJm\m:{ZTDMVHMh[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFE5KGi{czDifUBoemmnc4OgdoVi[3Srb36sJGlEPTBiPTCwMlg3KM7:TT6= NHfqVm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PFczPyd-MkGyPFg4Ojd:L3G+
HMC1 MkTKSpVv[3Srb36gZZN{[Xl? NVvXN3o4OSCqch?= MYTJcY12dm:vb3T1cIF1d3K7IHHjeIl3cXS7IHnuJIh2dWGwIFjNR|Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDwbI9z[m:uIEGyMY16emm|dHH0[UAyOy2jY3X0ZZRmN0F{M{G4O{1{fGmvdXzheIVlKEmOMT3i[ZRiKHOnY4LleIlwdiC2cnXheIVlKDFiaIKgdJJqd3JidH:gN|AhdWmwczDwbI9z[m:uIEGyMY16emm|dHH0[UAyOy2jY3X0ZZRmN0F{M{G4O{BkcGGubHXu[4Uh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>? M{TBU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{CyPVY4Lz5{MUOwNlk3PzxxYU6=
RAW264.7 M1K2[2FvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MorTNVAhfU1? MkO4NlQhcHK| NYTEZpVkSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDubZRzcXSnIHzleoVtKGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBIemmnc4OgcYV1cG:mLDDBZ5Rqfmm2eTC9JFEvPTRizszNMi=> NUj5eYpRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOVM2PDNpPkKxN|U{PTR|PD;hQi=>
RAW264.7 MoHlRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NWTNS|B6OThiaILz M2eyeGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmYu[WyyaHGgd4VkemW2aX;uJIFnfGW{IEG4JIhzeyxiSVO1NEA:KDBwMEGg{txONg>? M3zyNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{WzOVQ{Lz5{MUO1N|U1OzxxYU6=
RAW264.7 NUj2XI5ESW62aXnu[oxidW2jdH;yfUBie3OjeR?= NVP5SmRLOjRiaILz NWi3WVU1SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5MhdWW2aH;kMEBKSzVyIE2gNE45KM7:TT6= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN3M{W0N{c,OjF|NUO1OFM9N2F-
RAW264.7 NYPVXJBrSW62aXnu[oxidW2jdH;yfUBie3OjeR?= M2T3flI1KGi{cx?= NHzrRmRCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiSV\OMYdidW2jIHnu[JVk\WRiTl:gdJJw\HWldHnvckBi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYN1cW:wLDDJR|UxKD1iMD6wNkDPxE1w NHjSWm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO2NVM{QCd-MkGzOlE{Ozh:L3G+
RAW264.7 MYfGeY5kfGmxbjDhd5NigQ>? M2jKTVIxKG2rboO= NF;6Wm9KdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KHC{ZT3pcoN2[mG2ZXSg[o9zKDJyIH3pcpMh[mWob4LlJGxRWyClaHHscIVv\2VibXXhd5Vz\WRiMUigbJJ{KHCxc4SgUHBUKGOqYXzs[Y5o\SCkeTDHdolme3NibXX0bI9l NYCwWHlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3O|UyPTJpPkKxO|c2OTV{PD;hQi=>
RAW264.7 Mmn2SpVv[3Srb36gZZN{[Xl? NFeyfZU{OCCvaX7z M33LeWlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5H[WyyaHGgdJJw\HWldHnvckBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDwdoUucW6ldXLheIVlKG[xcjCzNEBucW6|IHLl[o9z\SCOUGOgZ4hidGynbnflJI1m[XO3cnXkJFYhcHK|IIDvd5QhVFCVIHPoZYxt\W6pZTDifUBGVEmVQR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5NUG1Nkc,OjF5N{WxOVI9N2F-
HeLa MWPGeY5kfGmxbjDhd5NigQ>? Ml6wRYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDI[WxiKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigcJVkcW[ncnHz[UBo\W6nIHzpcotm\CC2bzDNUXRXKHC{b33veIVzKGG|c3Xzd4VlKGG|IHz1Z4ln\XKjc3WgeJJidnOjY4TpeoF1cW:wIHHjeIl3cXS7LDDFR|UxKD1iMD6wNVch|ryPLh?= M3jZXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOU[zPVg3Lz5{MUm2N|k5PjxxYU6=
insect cells M3;tWWZ2dmO2aX;uJIF{e2G7 NFT4RmJFcXOybHHj[Y1mdnRib3[geIV1emGvZYTofYxzcG:mYX3pcoUudGGkZXzl[EBF\XijbXX0bIF{d26nIH\yc40hcHWvYX6gdoVkd22kaX7hcpQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG|c3H5MEBKSzVyIE2gNE4xODNizszNMi=> NXfLfoM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OlM6QDZpPkKxPVY{QTh4PD;hQi=>
insect cells NVezRXF3TnWwY4Tpc44h[XO|YYm= MWnEbZNxdGGlZX3lcpQhd2ZidHX0doFu\XSqeXzybI9l[W2rbnWtcIFj\WynZDDE[ZhidWW2aHHzc45mKG[{b32gbJVu[W5icnXjc41jcW6jboSgcYlv\XKjbH;jc5J1cWOxaXSgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG|c3H5MEBKSzVyIE2gNE4xOzNizszNMi=> NFXFNWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm2N|k5Pid-MkG5OlM6QDZ:L3G+
RAW264.7 MWrBcpRqcW6obHHtcYF1d3K7IHHzd4F6 MlXUNkBpenN? MVXBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLYP0bY12dGG2ZXSgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25icILleJJm[XSnZDDmc5IhOiCqcoOgZoVnd3KnIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVghcHK|IHL5JGdzcWW|czDt[ZRpd2RuIFnDOVAhRSByLkS3JO69VS5? M2LvTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{eyPVU3Lz5{MkO3Nlk2PjxxYU6=
RAW264.7 MmT4RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? MmfpNkBpenN? M4jjR2FvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCycnX0doVifGWmIH\vdkAzKGi{czDi[YZwemViTGDTJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjCxPEBpenNiYomgS5Jq\XO|IH3leIhw\CxiSVO1NEA:KDBwOUig{txONg>? NGf2UXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWzO|M3Oid-MkK1N|c{PjJ:L3G+
A549 M4rGVWFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NUPqWXk5OSC3TR?= M2L5O|MxKG2rboO= NGfId3NCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBKVC1zYnX0ZU1qdmS3Y3XkJI1RT0WVMTDlfJBz\XO|aX;uJIF1KDFidV2geJJm[XSnZDCzNEBucW6|IIDybY9zKHSxIFnMMVFj\XSjIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB2ODDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzJJJmdGG2aY\lJJRwKGOxboTyc4w> NVjXTJFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3PVU6ODBpPkKyO|k2QTByPD;hQi=>
H13 MXPGeY5kfGmxbjDhd5NigQ>? M4W1O|MhcHK| NEjxTIxKdmirYnn0bY9vKG:oIHfseYNw[2:{dHnjc4llKHKnY3XweI9zKGmwIHj1cYFvKEhzMzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGeudXPvZ49zfGmlb3nkMYlv\HWlZXSgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEOgbJJ{KGK7IGuzTH0ufGi7bXnkbY5mKGmwY3;ydI9z[XSrb36gZZN{[XluIFXDOVAhRSByLkCwPVch|ryPLh?= NFG4dlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5PVQ5PSd-MkOxPVk1QDV:L3G+
293 MSR NF[0NWpHfW6ldHnvckBie3OjeR?= NF3z[Vg3OCCvaX7z NHW0RYJFcXOybHHj[Y1mdnRib3[gX|NJZS2mZYjhcYV1cGG|b37lJIh2dWGwIHfseYNw[2:{dHnjc4llKHKnY3XweI9zKGGucHjhJIV5eHKnc4Pl[EBqdiB{OUOgUXNTKGOnbHzzJIFnfGW{IE[wJI1qdnNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiRVO1NEA:KDBwMEGyOUDPxE1w NGPVdGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5PVQ5PSd-MkOxPVk1QDV:L3G+
IOBA-NHC MmrNSpVv[3Srb36gZZN{[Xl? NGWwN3E1OCC3TR?= NUPZRWpOOjRiaILz NVjtdpJVUW6qaXLpeIlwdiCxZjDOSk1s[XCyYVKgdFY2NW2nZHnheIVlKEmOLU[gdoVt\WG|ZTDpckBJW1ZzIFvPV{Bqdm[nY4Tl[EBpfW2jbjDJU2JCNU6KQzDj[YxteyCjdDC0NEB2VSCjZoTldkAzPCCqcoOgZpkhTUyLU1G= MorRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{MUm4OVUoRjJ|MkG5PFU2RC:jPh?=
IOBA-NHC M4PHZmZ2dmO2aX;uJIF{e2G7 MXW0NEB2VQ>? M3LMOlI1KGi{cx?= M{PPPGlvcGmkaYTpc44hd2ZiTl[tb4FxeGGEIIC2OU1u\WSrYYTl[EBVVk[jbIDoZUBz\WynYYPlJIlvKEiVVkGgT29UKGmwZnXjeIVlKGi3bXHuJGlQSkFvTljDJINmdGy|IHH0JFQxKHWPIHHmeIVzKDJ2IHjyd{BjgSCHTFnTRS=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJzOUi1OUc,OjN{MUm4OVU9N2F-
IOBA-NHC MmnDSpVv[3Srb36gZZN{[Xl? NVPEd4Z5PDBidV2= MWOyOEBpenN? NHrjfphKdmirYnn0bY9vKG:oIF7GMYtieHCjQjDwOlUudWWmaXH0[YQhUUxvODDy[Yxm[XOnIHnuJGhUXjFiS1;TJIlv\mWldHXkJIh2dWGwIFnPRmEuVkiFIHPlcIx{KGG2IESwJJVOKGGodHXyJFI1KGi{czDifUBGVEmVQR?= NV35TnFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNVk5PTVpPkKzNlE6QDV3PD;hQi=>
HCLE M{L6R2Z2dmO2aX;uJIF{e2G7 NXXoNIZXPDBidV2= MU[yOEBpenN? NHjLRWVKdmirYnn0bY9vKG:oIF7GMYtieHCjQjDwOlUudWWmaXH0[YQhUUxvODDy[Yxm[XOnIHnuJGhUXjFiS1;TJIlv\mWldHXkJIh2dWGwIFjDUGUh[2WubIOgZZQhPDBidV2gZYZ1\XJiMkSgbJJ{KGK7IFXMTXNC MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJzOUi1OUc,OjN{MUm4OVU9N2F-
HCT116 NUXBN2VITnWwY4Tpc44h[XO|YYm= NFrYcVAzOCC2bzC0NEB2VQ>? MU[yOEBpenN? NY[1bVZ6SWexbnnzeEBi[3Srdnn0fUBifCCpbIXjc4NwenSrY3;p[EBz\WOncITvdkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBJS1RzMU[gZ4VtdHNiY3:t[ZhxemW|c3nu[{BIWkViYYSgNlAhfG9iNECgeW0h[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? NGHvemE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K3OlQ1QSd-MkOyO|Y1PDl:L3G+
RAW264.7 NEXVTmJCdnSraX7mcIFudWG2b4L5JIF{e2G7 MonkNkBpenN? M{jDOWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGmwY4XiZZRm\CCob4KgNkBpenNicILpc5IhfG9iTGDTMYNp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgS5Jq\XO|IH3leIhw\CxiSVO1NEA:KDFwNEOg{txONg>? MneyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{O5OlUoRjJ|M{ezPVY2RC:jPh?=
HeLaS3 M{XIcGZ2dmO2aX;uJIF{e2G7 NG\LN2ozKGi{cx?= MoTOSIl{eGyjY3Xt[Y51KG:oIGuzTH1F\XijbXX0bIF{d26nIH\yc40h\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGGodHXyJFIhcHK|LDDJR|UxKD1iMD6wNFQyKM7:TT6= NWq0bFJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFMxQDJpPkKzOFA{ODh{PD;hQi=>
HeLaS3 MoPWSpVv[3Srb36gZZN{[Xl? NHfqS|EzKGi{cx?= MWfEbZNxdGGlZX3lcpQhd2ZiW{PIYWRmgGGvZYToZZNwdmViZoLvcUBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDI[WxiWzNiY3XscJMh[W[2ZYKgNkBpenNuIFvpJF0hOC5yMEKyJO69VS5? MnfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEOwPFIoRjJ|NECzNFgzRC:jPh?=
A549 MVzGeY5kfGmxbjDhd5NigQ>? MlT2WJJidnO{ZYDy[ZN{cW:wIHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDJUFFj\XSjLYP0bY12dGG2ZXSgUmZs[XCyYVKt[IVx\W6mZX70JGUue2WuZXP0bY4hfHKjboPjdolxfGmxbjDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwNVEh|ryPLh?= NH\nfHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mxOlU6PCd-MkO5NVY2QTR:L3G+
A549 NH7RUnlHfW6ldHnvckBie3OjeR?= MWTUdoFve3KncILld5Nqd25iYXP0bZZqfHliYYSg[4x2[2:lb4L0bYNwcWRicnXj[ZB1d3JiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDNRU1{fGmvdXzheIVlKEGSMTDy[ZNxd26|ZTDlcIVu\W62IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECyOUDPxE1w MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlzNkW5OEc,OjN7MU[1PVQ9N2F-
Hela NUT5d215TnWwY4Tpc44h[XO|YYm= MW\UdoFve2GldHn2ZZRqd25iYXP0bZZqfHliYYSg[4x2[2:lb4L0bYNwcWRicnXj[ZB1d3JibHnnZY5lKGKrbnTpcoch\G:vYXnuJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKGi3bXHuJGhmdGFiY3XscJMh[29vZYjwdoV{e2mwZzDHRWw1KESQQTDibY5lcW6pIHTvcYFqdiCjc4Pld5Nm\CCjczDOVE0yNXO2aX31cIF1\WRiR2LFJIFkfGm4YYTpc44h[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODR3IN88UU4> MmnaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MU[1PVQoRjJ|OUG2OVk1RC:jPh?=
A549 M{HhZmZ2dmO2aX;uJIF{e2G7 NGXhTXlC\2:waYP0JIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBKVC1zYnX0ZU1qdmS3Y3XkJG5HNWujcIDhRkBl\XCnbnTlcpQhTS2|ZXzlZ5RqdiCjY4TpeoF1cW:wIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECxNUDPxE1w NYHDZZZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OVMxPzBpPkKzPVU{ODdyPD;hQi=>
A549 MlfRSpVv[3Srb36gZZN{[Xl? M{TyWWFod26rc4SgZYN1cX[rdImgZZQh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHBOSS2rbnT1Z4VlKEGSLUGgZYN1cX[jdHnvckBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlAxOjVizszNMi=> NHXUfYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m1N|A4OCd-MkO5OVMxPzB:L3G+
HeLa NF;vWWZHfW6ldHnvckBie3OjeR?= NVXJW5FKXHKjboPhZ5RqfmG2aX;uJI9nKGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIxq\2GwZDDibY5lcW6pIHTvcYFqdiBqdX7rco94diCxcnnnbY4qKHS{YX7z[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh[WO2aY\heIlwdiCxZjDOVE0yKGK7IFfBUFQhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNFQ2KM7:TT6= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl3M{C3NEc,OjN7NUOwO|A9N2F-
A549 MoXBSpVv[3Srb36gZZN{[Xl? Mnm5WJJidnO{ZYDy[ZN{cW:wIH;mJIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDQUWEucW6mdXPl[EBCWC1zIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECyOUDPxE1w MknNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUG2OFQoRjJ2MEGxOlQ1RC:jPh?=
HeLa M4DndmZ2dmO2aX;uJIF{e2G7 NHuxNWdVemGwc3HjeIl3[XSrb36gc4YhT1JvTFLEJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLXX4dJJme3OrbnegS2FNPC2GQlSgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNFQzKM7:TT6= M{jaZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEGxOlQ1Lz5{NECxNVY1PDxxYU6=
HEK293 M1T3dGZ2dmO2aX;uJIF{e2G7 NHq5WnlFcXOybHHj[Y1mdnRib3[gX|NJZS2jbHTvd5Rmem:wZTDmdo9uKGi3bXHuJI1qdmW{YXzvZ49zfGmlb3nkJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqKD1iMD6wNFA{PiEQvF2u Mn3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NE[3NlgoRjJ2NES2O|I5RC:jPh?=
HEK293 MnTlSpVv[3Srb36gZZN{[Xl? M1XUN2Rqe3CuYXPlcYVvfCCxZjDbN2heNWSneHHt[ZRp[XOxbnWg[pJwdSCqdX3hckBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDLbUA:KDBwMECwOlkh|ryPLh?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2NkeyPEc,OjR2NE[3Nlg9N2F-
HEK293 NYe4WVVWTnWwY4Tpc44h[XO|YYm= NInke2VFcXOybHHj[Y1mdnRib3[gX|NJZS2vZYTofYx1emmnbn;sc45mKG[{b32gbJVu[W5icILv[4V{fGW{b37lJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqKD1iMD61OlEh|ryPLh?= NHLFW3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES0OlczQCd-MkS0OFY4Ojh:L3G+
HEK293 MUHGeY5kfGmxbjDhd5NigQ>? MVjEbZNxdGGlZX3lcpQhd2ZiW{PIYU1u\XSqeXz0dolmdm:ub37lJIZzd21iaIXtZY4h[W6mcn;n[Y4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2liPTCxMlQyKM7:TT6= M2\IeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NES2O|I5Lz5{NES0OlczQDxxYU6=
HeLa NWTLVIoyTnWwY4Tpc44h[XO|YYm= NEPCZWtVemGwc3HjeIl3[XSrb36gc4Yh\2y3Y3;jc5J1cWOxaXSgdoVk\XC2b4KgbY4hcHWvYX6gTIVN[SClZXzsd{Bp[XKkb4LpcochVU2WVjDwdo9ud3SncjDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCxO{DPxE1w M4ricVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkW2OVY2Lz5{NE[1OlU3PTxxYU6=
A549 MoWzSpVv[3Srb36gZZN{[Xl? MVzB[49vcXO2IHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhTS2|ZXzlZ5RqdiC2cnHud5JmeHKnc4Ppc44h[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODFzIN88UU4> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl6MEC1N{c,OjR7OECwOVM9N2F-
A549 NVHLTo5{TnWwY4Tpc44h[XO|YYm= MmjCRYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KEGSLUGgeJJidnO{ZYDy[ZN{cW:wIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMECyOUDPxE1w Mm\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OECwOVMoRjJ2OUiwNFU{RC:jPh?=
HeLa MWTGeY5kfGmxbjDhd5NigQ>? MoHBRYdwdmm|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KE6SLUGgeJJidnOjY4TpeoF1cW:wIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEC0OUDPxE1w M3vlWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUiwNFU{Lz5{NEm4NFA2OzxxYU6=
B16F10 MWrBcpRqfHWvb4KgZZN{[Xl? NVHuZlVUOjBibXevb4c> NIjkXHozPCCmYYnz NX\qbJZKSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgRlE3TjFyIHPlcIx{KHinbn;ndoFnfGWmIHnuJGM2P0KOL{[gcY92e2ViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckB1fW2xcjDndo94fGhiYYSgNlAhdWdxa3esJIlxKG2nYYP1doVlKGGodHXyJFI1KGSjeYOgc4YhfHWvb4KgbY5w[3WuYYTpc44> NYXRT2xJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVIxPzFpPkK0PVkzODdzPD;hQi=>
B16F10 M2\GbmFvfGm2dX3vdkBie3OjeR?= M2jpOlIxKG2pL3vn NHLjZoszPCCmYYnz MV;BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEMU\GNVAh[2WubIOgfIVvd2e{YX\0[YQhcW5iQ{W3RmwwPiCvb4Xz[UBie3Onc4Pl[EBieyCjcH;weI91cWNicnXnbY9veyCjdDCyNEBu\y:tZzygbZAhdWWjc4Xy[YQh[W[2ZYKgNlQh\GG7czDv[kB1fW2xcjDpco9kfWyjdHnvckBjgSCWVV7FUEBie3OjeR?= MmjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUKwO|EoRjJ2OUmyNFcyRC:jPh?=
B16F10 M{LwWGFxd3C2b4Ppd{Bie3OjeR?= MUOxNEB2VQ>? MUm0PEBpenN? NU\HRWRIUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBud3W|ZTDCNVZHOTBiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gdoF1cW9ib3[gRmFZN0KlbEKg[ZhxemW|c3nvckBifCBzMDD1UUBi\nSncjC0PEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7MkC3NUc,OjR7OUKwO|E9N2F-
B16F10 M{fCTGFvfGm2dX3vdkBie3OjeR?= NUXYSohoOjBibXevb4c> NXzS[JhGOjRiZHH5dy=> MmXyRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQkG2SlExKGOnbHzzJJhmdm:pcnHmeIVlKGmwIFO1O2JNNzZibX;1d4Uh[XO|ZYPz[YQh[XNiYYDvdJRwfGmlIILl[4lwdnNiYYSgNlAhdWdxa3esJIlxKG2nYYP1doVlKGGodHXyJFI1KGSjeYOgc4YhfHWvb4KgbY5w[3WuYYTpc44h[nliVlWtZ4FlcGW{aX6= M1PnUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUmyNFcyLz5{NEm5NlA4OTxxYU6=
CUTLL1 M4jsN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGrTfVI1QCCqcoO= MXHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDDWXRNVDFiY3XscJMhf2m2aDDobYdpKEeUIHX4dJJme3Orb36gcIV3\Wy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigQ>? NV\pfo1GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOVAyPjBpPkK1NFUxOTZyPD;hQi=>
HEK293 M1PvVWZ2dmO2aX;uJIF{e2G7 M1rHS2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2NTKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xODF|IN88UU4> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNU[4PEc,OjV|MEW2PFg9N2F-
RAW264.7 MmPTSpVv[3Srb36gZZN{[Xl? MUmyOEBpenN? NF;admtKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGGodHXyJFI1KGi{czDifUBIemmnc4OgZZN{[XlicnXsZZRqfmVidH:geoVpcWOuZT30doVifGWmIHPvcpRzd2xuIFnDOVAhRSByLkig{txONg>? NVHhfmYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVk5QDJpPkK1OFk6QDh{PD;hQi=>
HFF MkG2SpVv[3Srb36gZZN{[Xl? MkXRRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWiCrbjDISmYh[2WubIOgZZN{\XO|ZXSgZZMhe3WycILld5Nqd25ib3[gTWwuOS2rbnT1Z4VlKEmOLU[gdJJw\HWldHnvckwhUUN3MDC9JFAvODBzIN88UU4> NWWyW2ZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NVY4QTFpPkK1OVE3PzlzPD;hQi=>
RAW264.7 MnPzRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NYLqNWl7OSCvTR?= NH;FTnc{OCCvaX7z MUnBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKG:oIFzQV{1qdmS3Y3XkJHRPTiCvUl7BJIV5eHKnc4Ppc44h[2WubIOgZZQhOSCvTTD0doVifGWmIEOwJI1qdnNiYnXmc5JmKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiMzDodpMh[nlicWLUMXBEWiCjbnHsfZNqew>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ4N{m2NEc,OjV4Nke5OlA9N2F-
SW1353 M3rnOGZ2dmO2aX;uJIF{e2G7 MYCyOEBpenN? MYnUdoFve3KncILld5Nqd25iYXP0bZZqfHliYYSg[4x2[2:lb4L0bYNwcWRicnXj[ZB1d3JiaX6gbJVu[W5iU2exN|U{KGOnbHzzJIF{e2W|c3XkJIF{KHKncILld5Nqd25ib3[gTWwuOS2rbnT1Z4VlKE2PUD2xN{Bxem:mdXP0bY9vKGGodHXyJFI1KGi{czDifUBGVEmVQTygTWM2OCB;IECuNFAyQCEQvF2u NYD1N4ZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFYyODBpPkK1O|A3OTByPD;hQi=>
RAW264.7 Ml;kSpVv[3Srb36gZZN{[Xl? MXO2JIhzew>? NIi0OYZKdmirYnn0bY9vKG:oIGTOSoFteGijIIDyc4R2[3Srb36gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiaX7jeYJifGWmIH\vdkA3KGi{czDifUB{[W6md3njbEBqdW23bn;hd5NigSxiSVO1NEA:KDBwMESg{txONg>? NFjrd4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOFY3Oid-MkW4NlQ3PjJ:L3G+
J774A.1 NFvEeI5HfW6ldHnvckBie3OjeR?= NEDQOpU3KGi{cx?= MXnJcohq[mm2aX;uJI9nKFSQRnHsdIhiKHC{b3T1Z5Rqd25iaX6gcY92e2ViSke3OGEvOSClZXzsd{BqdmO3YnH0[YQh\m:{IE[gbJJ{KGK7IIPhcoR4cWOqIHntcZVvd2G|c3H5MEBKSzVyIE2gNE41KM7:TT6= NEjsW4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOFY3Oid-MkW4NlQ3PjJ:L3G+
RAW264.7 MlzjSpVv[3Srb36gZZN{[Xl? MmHINVIhcHK| M3nISGlvcGmkaYTpc44hd2ZiSVytNYJmfGFicILv[JVkfGmxbjDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCrbnP1ZoF1\WRiZn;yJFEzKGi{czDifUB{[W6md3njbEBqdW23bn;hd5NigSxiSVO1NEA:KDRwNzFOwG0v M1jMTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK0OlYzLz5{NUiyOFY3OjxxYU6=
J774A.1 MlLKSpVv[3Srb36gZZN{[Xl? M{n6fFEzKGi{cx?= Mn;CTY5pcWKrdHnvckBw\iCLTD2xZoV1[SCycn;keYN1cW:wIHnuJI1wfXOnIFq3O|RCNjFiY3XscJMhcW6ldXLheIVlKG[xcjCxNkBpenNiYomgd4Fv\HerY3igbY1ufW6xYYPzZZktKEmFNUCgQUAzOiEQvF2u NWnGNnNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlQ3PjJpPkK1PFI1PjZ{PD;hQi=>
RAW264.7 Ml;5RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? M{\FcFI1KGi{cx?= M1v1[GFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCjZoTldkAzPCCqcoOgZpkhT3KrZYPzJIF{e2G7LDDJR|UxKD1iMD62N{DPxE1w M4fuOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEi3N|g1Lz5{NkC4O|M5PDxxYU6=
BV2 M3LLfmFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NEHV[ZozPCCqcoO= NUfze2w5SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCFNUfCUFYwUiCvb4Xz[UBDXjJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCwaYTybYMhd3irZHWgdJJw\HWldHnvckBi\nSncjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwJF0hOC5yMkWg{txONg>? NX\GW4RQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xNVA1PDNpPkK2NVExPDR|PD;hQi=>
RAW264.7 MY\GeY5kfGmxbjDhd5NigQ>? NWfEN3pkOjRiaILz NHK0Z|JKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGGodHXyJFI1KGi{czDifUBIemmnc4OgZZN{[XluIFnDOVAhRSByLkig{txONg>? NHnSTW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOwOVQxPid-Mk[zNFU1ODZ:L3G+
J774 NUPMe483SW62aXnu[oxidW2jdH;yfUBie3OjeR?= NVmwPHlkOTBidV2= NXfnfllVOjRiaILz NX;6NFc4SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFq3O|Qh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBqVk:VIHX4dJJme3Orb36gZZQhOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NXv5PXJKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5NVU6QThpPkK2PVE2QTl6PD;hQi=>
IM9 MUnGeY5kfGmxbjDhd5NigQ>? NYX2SodjTGm|cHzhZ4Vu\W62IH;mJHs{UF2mZYjhcYV1cGG|b37lJIZzd21iZ3z1Z49kd3K2aXPvbYQhemWlZYD0c5IhcW5iaIXtZY4hUU17IHPlcIx{NCCLQ{WwJF0hOC5yMEO4JO69VS5? NX;MTHI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PFg5ODFpPkK2PVg5QDBzPD;hQi=>
C57BL/6 NXXHZVBnSW62aXnu[oxidW2jdH;yfUBie3OjeR?= MWKxNEBu\y:tZx?= M33Zb|EhcHJ? M4LIXmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hSzV5QlyvOkBud3W|ZTDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIr5cY9{[W5vaX7keYNm\CCvb37vcpVkdGWjcjDj[YxteyCvaXfyZZRqd25iaX6gZZJ1cWO3bHHyJINifmm2eTDheEAyOCCvZz;r[{whcXBiYXTtbY5qe3SncnXkJFEhcHJicILpc5IhfG9ieontc5NidiClaHHscIVv\2VibXXhd5Vz\WRiYYSgOkB1dyB{NDDodpM> MoDaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Mke0OVkoRjJ5MkK3OFU6RC:jPh?=
C57BL/6 MlLSRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NWnlXYZDOTBibXevb4c> NYHFNmRMOSCqch?= M2T6UmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hSzV5QlyvOkBud3W|ZTDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIIr5cY9{[W5vaX7keYNm\CCrbn\pcJRz[XSrb36gc4YhcW6obHHtcYF1d3K7IHPlcIx{KGmwdH:gd5lvd3[rYXygeIl{e3WnIHH0JFExKG2pL3vnMEBqeCCjZH3pcol{fGW{ZXSgNUBpeiCycnnvdkB1dyC8eX3vd4FvKGOqYXzs[Y5o\SCvZXHzeZJm\CCjdDC2JJRwKDJ2IHjyd{BjgSCqZX3heI95gWyrbjDhcoQh\W:|aX6gd5RicQ>? M3\pOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MkK3OFU6Lz5{N{KyO|Q2QTxxYU6=
J774A.1 Mlf2RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? MUSxJJVO MXGxJIhz NIq3UXlCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJGo4PzSDLkGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC8eX3vd4FvKGGwZDDJSm5o[W2vYT3pcoR2[2WmIFnMOkBxem:mdXP0bY9vKGmwIHPlcIwu\nKnZTDzeZBmem6jdHHueEBifCBzIIXNJJBz\WmwY4XiZZRm\CCob4KgNUBpeiCob3zsc5dm\CCkeTD6fY1we2GwIHHu[EBKTk6pYX3tZUBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgd4Fv\HerY3igSWxKW0F? M4\RTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MkK3OFU6Lz5{N{KyO|Q2QTxxYU6=
J774A.1 M4[wSmFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NHzYdocyKHWP NYPX[VE6OSCqch?= M33OXGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgTlc4PEFwMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHq7bX;zZY4h[W6mIFnGUodidW2jLXnu[JVk\WRiQ1;YNkBmgHC{ZYPzbY9vKGG2IEGgeW0heHKnaX7jeYJifGWmIH\vdkAyKGi{IH\vcIxwf2WmIHL5JJp6dW:|YX6gZY5lKEmITnfhcY1iKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MlrlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{Mke0OVkoRjJ5MkK3OFU6RC:jPh?=
J774A.1 M2Hx[2FvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NVfm[opbOSC3TR?= NWPafpNEOSCqch?= NWXnfpV3SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBLPzd2QT6xJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2Zieontc5NidiCjbnSgTWZP\2GvbXGtbY5lfWOnZDDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCjdDCxJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhOSCqcjDmc4xtd3enZDDifUB7gW2xc3HuJIFv\CCLRl7nZY1u[SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkh[2:ub4LpcYV1emmlIFfybYV{eyC2ZYP0 MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ{N{S1PUc,Ojd{Mke0OVk9N2F-
RAW264.7 NXnRbIN4SW62aXnu[oxidW2jdH;yfUBie3OjeR?= MVeyOEBpenN? MUDBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHnuJJBz\XOnbnPlJI9nKEySUzDifUBIemmnc4OgZZN{[XluIFnDOVAhRSByLk[zJO69VS5? NWnHXmw3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke4NlU2PDVpPkK3PFI2PTR3PD;hQi=>
IM9 M{T6NmZ2dmO2aX;uJIF{e2G7 NVjBWJZ2TGm|cHzhZ4Vu\W62IH;mJHs{UF2mZYjhcYV1cGG|b37lJIZzd21iR2KgdoVk\XC2b4KgbY4hcHWvYX6gTW06KGOnbHzzMEBKSzVyIE2gNE4xODN6IN88UU4> M3\3W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEe2NlUxLz5{N{i3OlI2ODxxYU6=
HeLa NIjQSnZHfW6ldHnvckBie3OjeR?= M2r3dXRz[W6|cnXwdoV{e2mxbjDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcnnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDUVGEucW6mdXPl[EBkd2yuYXflcoF{\SCycn;tc5RmeiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjByMUW4OUDPxE1w MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB2M{e5Okc,OjhyNEO3PVY9N2F-
THP1 M3r6bWZ2dmO2aX;uJIF{e2G7 NUG0NmVFXHKjboPy[ZBz\XO|aX;uJIFkfGm4aYT5JIF1KGeudXPvZ49zfGmlb3nkJJJm[2WydH;yJIlvKGi3bXHuJHRJWDFiY3XscJMh[XO|ZYPz[YQh[XNicnXj[ZB1d3JvbXXkbYF1\WRiYX70bU1qdm[uYX3tZZRwenliYXP0bZZqfHliYomgcYVie3W{aX7nJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgTWwuQCCycn;keYN1cW:wLDDJR|UxKD1iMD6wNFI2OTJizszNMi=> M{\iXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MESzO|k3Lz5{OEC0N|c6PjxxYU6=
HeLa MYPGeY5kfGmxbjDhd5NigQ>? MXfUdoFve2GldHn2ZZRqd25iYXP0bZZqfHliYYSg[4x2[2:lb4L0bYNwcWRicnXj[ZB1d3JiaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiTV3UWkBxem:vb4TldkBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlAxPzl2MzFOwG0v MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB2M{e5Okc,OjhyNEO3PVY9N2F-
Sf9 MmCxSpVv[3Srb36gZZN{[Xl? NVzJ[Ig5SmmwZHnu[{Bi\m[rbnn0fUB1dyC{ZXPvcYJqdmGwdDDoeY1idiCpbIXjc4NwenSrY3;p[EBz\WOncITvdkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgd4Y6KGOnbHzzMEBKSzVyIE2gNE4xOTJ3OTFOwG0v MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB2M{e5Okc,OjhyNEO3PVY9N2F-
RAW264.7 NUTUeVh4SW62aXnu[oxidW2jdH;yfUBie3OjeR?= M{X3VlMxKG2rboO= M3rzUWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44heHKndILlZZRm\CCob4KgN|AhdWmwczDmc4xtd3enZDDifUBNWFNic4TpcZVt[XSrb36g[o9zKDJ2IHjyd{BjgSCJcnnld5Mh[XO|YYmsJGlEPTBiPTC3JO69VS5? M{\YWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEm5O|M{Lz5{OES5PVc{OzxxYU6=
RAW264.7 Mn\nSpVv[3Srb36gZZN{[Xl? M2LpZ|I1KGi{cx?= M{O5SGlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bi\nSncjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwJF0hOi53IN88UU4> MlT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3OUi2N|MoRjJ6NUm4OlM{RC:jPh?=
Mino NFrBWVFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MV6xNFAhfU1? NYTtTXA5PDhiaILz MoTNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPaX7vJINmdGy|IHH0JFExOCC3TTDwdoVqdmO3YnH0[YQh\m:{IES4JIhzeyCob3zsc5dm\CCkeTD0bJlucWSrbnWgZYRlcXSrb36gcYVie3W{ZTDh[pRmeiBzNjDodpMh[nliVHj5cYllcW6nIHnuZ49zeG:{YYTpc44h[XO|YYm= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh3OEWxNUc,Ojh6NUi1NVE9N2F-
RAW264.7 Ml\JRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NX3MVVduSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHL5JIdzcWW|czDhd5NigSxiSVO1NEA:KDBwODFOwG0v MkTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{N{KxNlYoRjJ7MkeyNVI3RC:jPh?=
RAW264.7 MYXGeY5kfGmxbjDhd5NigQ>? NW[xSVNFOTBidV2= NHz0NYEyKGi{ NWXCR|c6SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gUHBUNXO2aX31cIF1\WRibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDOSk1s[XCyYVKgd4lodmGuaX7nJIJ6KG2nYYP1dolv\yC{ZXT1Z5Rqd25iaX6gR29ZOiCvUl7BJIxmfmWuIHH0JFExKHWPIIDy[ZRz\WG2ZXSge4l1cCClb33wc5Vv\CCob4KgNUBpeiCob3zsc5dm\CCkeTDMVHMhe3SrbYXsZZRqd25iZn;yJFYhcHK|IHL5JHJVNVCFUjDhcoFtgXOr NXrJNVRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzPFkyOjFpPkK5N|g6OTJzPD;hQi=>
RAW264.7 NWG5N5JwTnWwY4Tpc44h[XO|YYm= NHzGPJYyOCC3TR?= MVuxJIhz MXzB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKE6ILXvhdJBiSiC|aXfuZYxqdmdiYomgcYVie3W{aX7nJJJm\HWldHnvckBqdiCrTl;TJI1TVkFibHX2[Ywh[XRiMUCgeW0heHKndILlZZRm\CC5aYToJINwdXCxdX7kJIZweiBzIHjyJIZwdGyxd3XkJIJ6KEySUzDzeIlufWyjdHnvckBnd3JiNjDodpMh[nliUmStVGNTKGGwYXz5d4k> M{D3clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIyLz5{OUO4PVEzOTxxYU6=
RAW264.7 MYrGeY5kfGmxbjDhd5NigQ>? M33oSlExKHWP M3f4RVEhcHJ? NGPI[HdC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIF7GMYtieHCjQjDzbYdv[WyrbnegZpkhdWWjc4XybY5oKHKnZIXjeIlwdiCrbjDUUmZidHCqYTDtVm5CKGyndnXsJIF1KDFyIIXNJJBz\XS{ZXH0[YQhf2m2aDDjc41xd3WwZDDmc5IhOSCqcjDmc4xtd3enZDDifUBNWFNic4TpcZVt[XSrb36g[o9zKDZiaILzJIJ6KFKWLWDDVkBidmF? MkO0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|OEmxNlEoRjJ7M{i5NVIyRC:jPh?=
RAW264.7 NILZNW5HfW6ldHnvckBie3OjeR?= NX20RVhlOTBidV2= MUixJIhz MmTMRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCQRj3rZZBx[UJic3nncoFtcW6pIHL5JI1m[XO3cnnu[{Bz\WS3Y4Tpc44hcW5iSVytNYJmfGFibWLORUBt\X[nbDDheEAyOCC3TTDwdoV1emWjdHXkJJdqfGhiY3;tdI92dmRiZn;yJFEhcHJiZn;scI94\WRiYomgUHBUKHO2aX31cIF1cW:wIH\vdkA3KGi{czDifUBTXC2SQ2KgZY5i MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNUc,Ojl|OEmxNlE9N2F-
RAW264.7 NXTlbXZJTnWwY4Tpc44h[XO|YYm= NHW4e|kyOCC3TR?= MV6xJIhz NHTQOnBC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIF7GMYtieHCjQjDzbYdv[WyrbnegZpkhdWWjc4XybY5oKHKnZIXjeIlwdiCrbjDJUE03KG2UTlGgcIV3\WxiYYSgNVAhfU1icILleJJm[XSnZDD3bZRpKGOxbYDveY5lKG[xcjCxJIhzKG[xbHzve4VlKGK7IFzQV{B{fGmvdXzheIlwdiCob4KgOkBpenNiYomgVnQuWEOUIHHuZYx6e2l? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNUc,Ojl|OEmxNlE9N2F-
RAW264.7 MXLGeY5kfGmxbjDhd5NigQ>? MUCxNEB2VQ>? MX[xJIhz M2LMfGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFzQV{1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTl[tb4FxeGGEIIPp[45idGmwZzDifUBu\WG|dYLpcochemWmdXP0bY9vKGmwIF7PJJBzd2S3Y4Tpc44h[XRiMUCgeW0heHKndILlZZRm\CC5aYToJINwdXCxdX7kJIZweiBzIHjyJIZwdGyxd3XkJIJ6KEySUzDzeIlufWyjdHnvckBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgS5Jq\Q>? NHLXPWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUO4PVEzOSd-MkmzPFkyOjF:L3G+
RAW264.7 NFz5UmRHfW6ldHnvckBie3OjeR?= Ml\jNVAhfU1? MmLlNUBpeg>? M3fsbWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFzQV{1{fGmvdXzheIVlKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTl[tb4FxeGGEIIPp[45idGmwZzDifUBu\WG|dYLpcochemWmdXP0bY9vKGmwIFnMMVFj\XSjIIDyc4R2[3Srb36gZZQhOTBidV2gdJJmfHKnYYTl[EB4cXSqIHPvcZBwfW6mIH\vdkAyKGi{IH\vcIxwf2WmIHL5JGxRWyC|dHnteYxifGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[g>? NXrnRW5mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzPFkyOjFpPkK5N|g6OTJzPD;hQi=>
RAW264.7 M3n0V2Z2dmO2aX;uJIF{e2G7 M{KyPFExKHWP MWixJIhz MVXB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKE6ILXvhdJBiSiC|aXfuZYxqdmdiYomgcYVie3W{aX7nJJJm\HWldHnvckBqdiCLTD22JJBzd2S3Y4Tpc44h[XRiMUCgeW0heHKndILlZZRm\CC5aYToJINwdXCxdX7kJIZweiBzIHjyJIZwdGyxd3XkJIJ6KEySUzDzeIlufWyjdHnvckBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomgSWw> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNUc,Ojl|OEmxNlE9N2F-
RAW264.7 NVT3fI06TnWwY4Tpc44h[XO|YYm= MlzpNVAhfU1? MX6xJIhz MV3B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKE6ILXvhdJBiSiC|aXfuZYxqdmdiYomgcYVie3W{aX7nJJJm\HWldHnvckBqdiCWTl\hcJBp[SCycn;keYN1cW:wIHH0JFExKHWPIIDy[ZRz\WG2ZXSge4l1cCClb33wc5Vv\CCob4KgNUBpeiCob3zsc5dm\CCkeTDMVHMhe3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOyCqcoOgZpk> MnzyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|OEmxNlEoRjJ7M{i5NVIyRC:jPh?=
RAW264.7 NEHDcGlHfW6ldHnvckBie3OjeR?= M{PWb|ExKHWP M4CwcFEhcHJ? MoLiRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCQRj3rZZBx[UJic3nncoFtcW6pIHL5JI1m[XO3cnnu[{Bz\WS3Y4Tpc44hcW5iaV7PV{Bxem:2ZXnuJIxmfmWuIHH0JFExKHWPIIDy[ZRz\WG2ZXSge4l1cCClb33wc5Vv\CCob4KgNUBpeiCob3zsc5dm\CCkeTDMVHMhe3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6 NHLCcnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUO4PVEzOSd-MkmzPFkyOjF:L3G+
SK-N-MC MYnxTHRUKGG|c3H5 NVHtfZlmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BV2 NYjzWHQ4SW62aXnu[oxidW2jdH;yfUBie3OjeR?= NFnsZ2VCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJGJXOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxRWy2rbnT1Z4VlKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uMEBKSzVyIE2gPU42KM7:TT6= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0NEc,Ojl2OEK5OFA9N2F-
RAW264.7 MoX3SpVv[3Srb36gZZN{[Xl? MUiyOEBpenN? M4[ye2lvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigSxiSVO1NEA:KDFyLkeg{txONg>? M3PkNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUG4N|I3Lz5{OUWxPFMzPjxxYU6=
HeLa NXrFSZRyTnWwY4Tpc44h[XO|YYm= MV[wMlQhfU1? NY\rcpA5OjRiaILz MVHUdoFve3KncILld5Nqd25ib3[gS2NTKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgd5VxeHKnc4Ppc44hd2ZiRFXYMYlv\HWlZXSgS2lNYiCvUl7BJIV5eHKnc4Ppc44h[XRiMD60JJVOKGGodHXyJFI1KGi{czDifUBV[XGvYX6tZoF{\WRicnXhcE11cW2nIGDDVkBidmGueYPpdy=> NVLN[3JWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2O|A3QTdpPkK5OlcxPjl5PD;hQi=>
RAW264.7 NIHtR29CdnSraX7mcIFudWG2b4L5JIF{e2G7 MXzBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiTl:gdJJw\HWldHnvckwhUUN3MDC9JFgvOyEQvF2u NWSxT2k4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3OFE{PzJpPkK5O|QyOzd{PD;hQi=>
HEK 293T NX;Mc251TnWwY4Tpc44h[XO|YYm= NYLiU4VVSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0JGRPSS2kaX7kbY5oKGSxbXHpckBnfXOnZDDHVkApfW6tbn;3ckBwemmpaX6pJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gWWFUNWKuYTDISWshOjl|VDDj[YxteyCjc4Pld5Nm\CCjczDi[ZRiNWyjY4ThcYF{\SC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCkeTDGVmVVNWKjc3XkJGdmdmWETFH6[ZIh[XO|YYmsJGlEPTBiPTCwMlAxOjN3IN88UU4> NVrSeoZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxOFQ3QTdpPkOwNVQ1Pjl5PD;hQi=>
RAW264.7 MUjBcpRqcW6obHHtcYF1d3K7IHHzd4F6 MWiyNEB2VQ>? MUi2JIhzew>? MnvpRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhUUxvNjDwdo9lfWO2aX;uJIF1KDJyIIXNJJBz\XS{ZXH0[YQh\m:{IE[gbJJ{KG[xbHzve4VlKGK7IFzQV{B{fGmvdXzheIlwdiCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkhTUyLU1G= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNUK1OUc,OzB{MUWyOVU9N2F-
RAW264.7 NI\NTW9CdnSraX7mcIFudWG2b4L5JIF{e2G7 NXzSWVJTOjBidV2= NHvqdJo3KGi{cx?= MVrBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBKVC1zYnX0ZUBxem:mdXP0bY9vKGG2IEKwJJVOKHC{ZYTy[YF1\WRiZn;yJFYhcHK|IH\vcIxwf2WmIHL5JGxRWyC|dHnteYxifGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliRVzJV2E> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNUK1OUc,OzB{MUWyOVU9N2F-
RAW264.7 M3T6R2FvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MUWyNEB2VQ>? M3PJTlYhcHK| M3nob2FvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxRWy2rbnT1Z4VlKG6rdILpeIUheHKxZIXjeIlwdiCjdDCyNEB2VSCycnX0doVifGWmIH\vdkA3KGi{czDmc4xtd3enZDDifUBNWFNic4TpcZVt[XSrb36gcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNC NVXi[JQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNVUzPTVpPkOwNlE2OjV3PD;hQi=>
RAW264.7 NYLW[FZYSW62aXnu[oxidW2jdH;yfUBie3OjeR?= NYG2O|FuOjBidV2= MU[2JIhzew>? MkDnRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhXE6IYXzwbIEheHKxZIXjeIlwdiCjdDCyNEB2VSCycnX0doVifGWmIH\vdkA3KGi{czDmc4xtd3enZDDifUBNWFNic4TpcZVt[XSrb36gcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNC M2nRd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkG1NlU2Lz5|MEKxOVI2PTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Bcl-2 / Bcl-xl / Mcl-1 / Survivin / IAP1 ; 

PubMed: 28696369     


Bcl-xl, Bcl-2, Survivin, Mcl-1, and IAP1 protein levels were determined via Western blot analysis.

Cyclin D1 / Cyclin E / COX-2 / MMP-9 ; 

PubMed: 28696369     


Cyclin D1, Cyclin E, COX-2, and MMP-9 protein levels were determined via Western blot analysis.

Bax / p21; 

PubMed: 28696369     


The protein levels of Bax and p21 were determined via Western blot analysis.

p-JNK / JNK / p-p38 / p38 / p-ERK / ERK ; 

PubMed: 28696369     


The cells were treated with 100 μM of DHAP for the time periods stated; whole-cell extracts were then prepared and analyzed via Western blot analysis for p-p38 (Thr180/Tyr182), p-JNK (Thr183/Tyr185), and p-ERK1/2 (Thr202/Tyr204) by Western blot analysis. To confirm equal protein loading, the immunoblot was stripped and reprobed for JNK, p38, and ERK1/2. The results shown are representative of the three independent experiments

28696369
体内研究 在肺泡上皮细胞中,Dexamethasone肌肉注射给药到怀孕母羊,导致了以下结果(1)血压不变; (2)如先前在胎儿报道,感光度为内皮素-1(ET)的增加; (3)乙酰胆碱诱导的舒张是增加的; (4)L-NAME对ET可抑制血管舒张反应被废除; (5)存在于内皮依赖性血管舒张功能没有变化;(6)相比于盐水治疗的对照组,eNOS的RNA和蛋白水平没有变化。 [3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 79 mg/mL (201.29 mM)
Water Insoluble
Ethanol '''6 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+45% PEG 300+H2O
13mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 392.46
化学式

C22H29FO5

CAS号 50-02-2
储存条件 粉状
溶于溶剂
别名 NSC 34521, Hexadecadrol, Prednisolone F

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04911361 Not yet recruiting Drug: Dexamethasone Dry Eye Research Insight LLC July 26 2021 Phase 4

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I'm loonkig for Dexamethasone to use in cell culture, would it be better to use the Dexamethasone S1322, the sodiume phosphate S4028 or the acetate S3124?

  • 回答:

    S1322 is the free base form of Dexamethasone, S4028 is the sodium phosphate form and S3124 is the acetate salt. All of them have similar biological activity in tissue culture, however, their suitabilities vary in different solvents. As free base, S1322 has high solubility in DMSO (79mg/ml), poor solubility in water (<1mg/ml), and moderate solubility in ethanol (6mg/ml). As sodium phosphate salt, S4028 has high solubility in water (103mg/ml) but is insoluble in DMSO and ethanol (<1mg/ml). The solubility of S3124 is 87mg/ml in DMSO, <1mg/ml in water, and 20mg/ml in ethanol. Selection can be made based on the customer's experimental setup.

相关IL Receptor产品

Tags: 购买Dexamethasone (MK-125) | Dexamethasone (MK-125) ic50 | Dexamethasone (MK-125)价格 | Dexamethasone (MK-125) DMSO溶解度 | 采购Dexamethasone (MK-125) | Dexamethasone (MK-125)生产 | Dexamethasone (MK-125) 小鼠 | Dexamethasone (MK-125)化学结构 | Dexamethasone (MK-125)分子量 | Dexamethasone (MK-125) molecular weight | Dexamethasone (MK-125)说明书 | Dexamethasone (MK-125)供应商 | Dexamethasone (MK-125)体内 | Dexamethasone (MK-125)细胞系 | Dexamethasone (MK-125)浓度 | Dexamethasone (MK-125) nmr | Dexamethasone (MK-125)核磁 | Dexamethasone (MK-125)半数抑制浓度 | Dexamethasone (MK-125)体外
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID