Adalimumab (anti-TNF-alpha)

目录号:A2010 别名: LU200134, D2E7 中文名称:阿达木单抗

For research use only.
Not for use in humans.

Adalimumab (anti-TNF-alpha)是靶向TNF-alpha的人源重组单克隆抗体,抑制TNF-alpha。MW: 144.19 KD。

规格 价格 库存 购买数量  
RMB 5790.72 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck该产品发表文献4篇:

质量管理及产品安全说明书

TNF-alpha抑制剂选择性比较

生物活性

产品描述 Adalimumab (anti-TNF-alpha)是靶向TNF-alpha的人源重组单克隆抗体,抑制TNF-alpha。MW: 144.19 KD。
靶点
TNF-α [1]
()
体外研究

Adalimumab (anti-TNF-alpha)阻止TNF与p55和p75细胞表面TNF受体相互作用,因而中和这些细胞因子活性。Adalimumab减少MMP-1和MMP-3的浓度以及其他软骨和滑膜增生的标记,并减少炎症急性期反应物的浓度(CRP和ESR)和血清细胞因子(IL-1β mRNA、IL-1受体拮抗剂、IL-6)[1]

体内研究 Adalimumab (anti-TNF-alpha)安全性和耐受性良好。在健康的成年人体内,通过皮下注射40 mg adalimumasb,其平均生物利用度的绝对值为64%。在滑液中的浓度是其在血浆中的31-96%。最大血浆浓度在给药后131(±56)小时出现,平均半衰期为2周(10-20天)[1]。Adalimumab的处理可减缓支气管肺泡灌洗液(BAL)中OVA-诱导的血浆中IgE、TH2和TH1来源的炎症因子(如IL-4和IFN-γ)增加、抑制BAL和肺中炎症细胞的招募、抑制BAL中性白细胞的增多。它还能够改善杯状细胞化生和支气管纤维化[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: Adalimumab induces apoptosis of THP-1 cells
    Cells: THP-1 cells
    Concentrations: 10μg/mL
    Incubation Time: 6, 12, 18, 24, 30 hours
    Method: THP-1 cells (0.5×106) were incubated with anti-FcRI mAb and anti-FcRII mAb, and then cultured with adalimumab, infliximab, huIgG or vehicle (PBS) alone as indicated. LPS (1μg/mL) was added in all culture conditions. Apoptosis was analyzed at different time points by staining with annexin V-FITC and propidium iodide, Apo2.7-PE-conjugated antibody, or 7AAD.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/16374254

    Objective: Effect of TNF-α inhibition with adalimumab on endothelial adhesion molecules
    Cells: HUVECs (Human Umbilical Vein Endothelial cells), THP-1 cells
    Concentrations: 1 μg/ml
    Incubation Time: 6 hours
    Method: HUVECs were incubated with conditioned media from oxLDL-stimulated THP-1 cells (oxLDL CM) with or without adalimumab , and mRNA expression of the adhesion molecules VCAM-1, ICAM-1 and E-selectin in HUVECs was determined by real time PCR.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27467817

    Adalimumab can apply to mice (eg: CD1 mice, DBA/1 mice), and various in vitro assays (Only for Reference)

动物实验:
- 合并
  • Objective: Adalimumab ameliorates OVA-induced airway inflammation in mice
    Animal Models: CD1 mice
    Formulation: PBS
    Dosages: 1mg/100 g b.wt
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/27262379

    Objective: The distribution of certolizumab pegol, adalimumab, and infliximab in the inflamed paws of mice with collagen-induced arthritis
    Animal Models: DBA/1 mice
    Formulation: PBS
    Dosages: 2 mg/kg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19567252

    Adalimumab can apply to mice (eg: CD1 mice, DBA/1 mice), and various in vitro assays (Only for Reference)

抗体信息

CAS No. 331731-18-1
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技术支持

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

TNF-alpha Signaling Pathway Map

相关TNF-alpha产品

Tags: 购买Adalimumab (anti-TNF-alpha) | Adalimumab (anti-TNF-alpha)供应商 | 采购Adalimumab (anti-TNF-alpha) | Adalimumab (anti-TNF-alpha)价格 | Adalimumab (anti-TNF-alpha)生产 | 订购Adalimumab (anti-TNF-alpha) | Adalimumab (anti-TNF-alpha)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID