Necrostatin-1

For research use only. Not for use in humans.

目录号:S8037 别名: Nec-1

Necrostatin-1 Chemical Structure

CAS No. 4311-88-0

Necrostatin-1 (Nec-1)是一种特异性RIP1 (RIPK1)抑制剂,抑制TNF-α诱导的细胞坏死,在293T细胞中EC50为490 nM。Necrostatin-1也可抑制 IDO、细胞自噬和凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1054.09 现货
RMB 814.14 现货
RMB 2224.79 现货
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客户使用Selleck生产的Necrostatin-1发表文献112篇:

产品安全说明书

RIP kinase抑制剂选择性比较

生物活性

产品描述 Necrostatin-1 (Nec-1)是一种特异性RIP1 (RIPK1)抑制剂,抑制TNF-α诱导的细胞坏死,在293T细胞中EC50为490 nM。Necrostatin-1也可抑制 IDO、细胞自噬和凋亡。
特性 Necrostatin-1是抑制细胞坏死的有力工具。
靶点
RIP1 [1]
(293T cells)
490 nM(EC50)
体外研究

Necrostatin-1 (1-100 μM) 抑制过表达和内源性的RIP1发生自磷酸化。RIP1是初级细胞靶点,负责Necrostatin-1的抗细胞坏死活性。[1] Necrostatin-1有效抑制多种类型细胞触发的坏死性细胞死亡。Necrostatin-1作为细胞坏死的小分子抑制剂, 作用于jurkat细胞,抑制RIP激酶的诱导细胞坏死,抑制TNF-α诱导的细胞坏死,EC50为490 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-22 Mk\TR5l1d3SxeHnjbZR6KEG|c3H5 NWjOXWVzOTBizszN NYDBe5M5OTJiaB?= NVTKbW81eHKxdHXjeJMh[WejaX7zeEBk\WyuIHTlZZRpKGmwZIXj[YQh[nliNdMgcY1wdC:OIHfseZRidWG2ZdMg MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd4MEi2PUc,OTd5NkC4Olk9N2F-
Jurkat  NUPmRolQTnWwY4Tpc44hSXO|YYm= NFe2WnEzODEEoN88cS=> NHTObWE{OCCvaX6= MlHrdoVlfWOnc9MgUoFm\2yncnnhJIZwf2yncnmtbY5lfWOnZDDy[YFkfGm4ZTDvfJlo\W5ic4DlZ4lmeyBqUl;TLUBo\W6ncnH0bY9v NEK2N2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWzOVAzOCd-MkG1N|UxOjB:L3G+
Jurkat  M3fDcGN6fG:2b4jpZ4l1gSCDc4PhfS=> NGrXcHI2OC9iMUCwM|IxOMLizszt NYS0cHFxOS9|IHi= M1XIe5Jm\HWlZYRCpG5i\WeuZYLpZUBnd3euZYLpMYlv\HWlZXSgZ5l1d3SxeHnjbZR6 NV3qSVR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1N|UxOjBpPkKxOVM2ODJyPD;hQi=>
SW13 NVT5O2tTS2WubDDWbYFjcWyrdImgRZN{[Xl? MmOxNVAxKM7:TR?= MYiyOEBp MljISG1UVw>? NXLNdFllcW6lcnXhd4V{KGOnbHz1cIFzKHO3co\peoFt MnGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzM{[4NVgoRjJ{MUO2PFE5RC:jPh?=
8505c NXHwXoNuS2WubDDWbYFjcWyrdImgRZN{[Xl? NUTjVpVROTByIN88US=> MWSyOEBp NWjld29UTE2VTx?= NIPJbpJqdmO{ZXHz[ZMh[2WubIXsZZIhe3W{dnn2ZYw> M4O1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUO2PFE5Lz5{MkGzOlgyQDxxYU6=
TPC-1 MmHnR4VtdCCYaXHibYxqfHliQYPzZZk> NGrkblgyODBizszN MkTiNlQhcA>? M3XNSWROW09? NHftRXFqdmO{ZXHz[ZMh[2WubIXsZZIhe3W{dnn2ZYw> NUHaSJhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxN|Y5OThpPkKyNVM3QDF6PD;hQi=>
L929sA NY\xfVJISXCxcITvd4l{KEG|c3H5 NUXUeHZEOTBizszN NHTj[Y4yKGh? M{G1W4Fjem:pYYTld{B1cGViaX70[ZJi[3Srb36gc4Yh[2G|cHHz[U05KHerdHigSmFFTA>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4Mke2O{c,OjJ|NkK3Olc9N2F-
L929sA NFT5cmZCeG:ydH;zbZMhSXO|YYm= MkXZNVAh|ryP NFG4[GMyKGh? NVPucohpemW|Y4Xld{Bk\WyuczDlfJBz\XO|aX7nJHJKWEtzzqTJSEBnem:vIGTOSk1qdmS3Y3XkJIFxd3C2b4Ppdy=> NYLwdpRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOlI4PjdpPkKyN|YzPzZ5PD;hQi=>
L929sA NIX6fpFCeG:ydH;zbZMhSXO|YYm= MYOxNEDPxE1? NWjWZot[OSCq NWL1W5hbcW6qaXLpeJMhfGinIHHwc5B1d3SrYzDy[ZNxd26|ZTD0c{BVVkZ? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4Mke2O{c,OjJ|NkK3Olc9N2F-
K562/Adr  NI\FVY9HfW6ldHnvckBCe3OjeR?= MnnyOlAh|ryP NEK2WYcyOiCq MVfpcoNz\WG|ZYOgeIhmKGGldHn2bZR6KG:oIHPhd5Bie2W|LDDjZZNx[XOnIEigZY5lKDl? Mm\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
K562 NEnzcGZHfW6ldHnvckBCe3OjeR?= NH\jemE3OCEQvF2= MoLCNVIhcA>? M1zLbolv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= NWPFOoF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
HL60/Adr Ml\DSpVv[3Srb36gRZN{[Xl? MoHVOlAh|ryP MoLKNVIhcA>? MXnpcoNz\WG|ZYOgeIhmKGGldHn2bZR6KG:oIHPhd5Bie2W|LDDjZZNx[XOnIEigZY5lKDl? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|N{[4PUc,OjJ6M{e2PFk9N2F-
HL60 MYfGeY5kfGmxbjDBd5NigQ>? MVK2NEDPxE1? MVSxNkBp MmPabY5kemWjc3XzJJRp\SCjY4Tpeol1gSCxZjDjZZNx[XOnczygZ4F{eGG|ZTC4JIFv\CB7 MkfiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
K562/Adr  MYTGeY5kfGmxbjDBd5NigQ>? MnfLOlAh|ryP NYe2VYdlOTJiaB?= Mk\GZZVodWWwdIOgeIhmKGOjc4Dhd4UuOyCjY4Tpeol1gQ>? NYjKPGN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
K562 NU\3dJlTTnWwY4Tpc44hSXO|YYm= NFn6VJk3OCEQvF2= NXTyWpFLOTJiaB?= MVrheYdu\W62czD0bIUh[2G|cHHz[U0{KGGldHn2bZR6 M3TGS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60/Adr MnXCSpVv[3Srb36gRZN{[Xl? MnL6OlAh|ryP MlPWNVIhcA>? NFXqUJRifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 NUWw[pVRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
HL60 NEnabYxHfW6ldHnvckBCe3OjeR?= Mmf0OlAh|ryP MVWxNkBp MoTkZZVodWWwdIOgeIhmKGOjc4Dhd4UuOyCjY4Tpeol1gQ>? M3rqelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
K562/Adr  NWDvdoNvSXCxcITvd4l{KEG|c3H5 NF62c|M3OCEQvF2= M{LjZlEzKGh? MkTM[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|N{[4PUc,OjJ6M{e2PFk9N2F-
K562 NYS2dIdPSXCxcITvd4l{KEG|c3H5 M4WzUlYxKM7:TR?= NF20O4wyOiCq NWLDTpJN\W6qYX7j[ZMhe2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZM> NFXvVGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
HL60/Adr M{LPbWFxd3C2b4Ppd{BCe3OjeR?= MljPOlAh|ryP NHvKS2IyOiCq M4H0UYVvcGGwY3XzJJNpcWuxbnnuMYlv\HWlZXSgZZBweHSxc3nz M3jUb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60 NGLvTm1CeG:ydH;zbZMhSXO|YYm= M1TSeFYxKM7:TR?= MWKxNkBp M2fKXIVvcGGwY3XzJJNpcWuxbnnuMYlv\HWlZXSgZZBweHSxc3nz M3nlSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
SH-EP NUftWoh7SXCxcITvd4l{KEG|c3H5 M2PuVlEx6oDLzszNxsA> NYrp[Xk{PzMkgJnoxsA> M3nGdolvcGmkaYTzJGlCWCCrbnjpZol1d3JvIHHu[EBN\XijdIXteY1i[i2rbnT1Z4VlKGGyb4D0c5Nqew>? M4LBT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEmwN|IzLz5{Mki5NFMzOjxxYU6=
NIH3T3  MYrGeY5kfGmxbjDBd5NigQ>? NIX5UXUyOC93MDFOwG0> NWSxOYp[OS9|IHi= NIXZU25idWWuaX;yZZRmeyCWTldOtU1lemm4ZX6gZ49ueGyneDDmc5Ju[XSrb36= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4MU[3O{c,OjN{NkG2O|c9N2F-
HT-22  NV;k[IZbTnWwY4Tpc44hSXO|YYm= MUeyOeKh|ryP NVv6Z|NNOOLCk{OwxsBucW5? M33H[mROW09? NFX0[XdqdmirYnn0d{BGWktiQXP0bZZifGmxbjDpcoR2[2WmIHL5JIdtfXSjbXH0[S=> MnexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MEe3OVIoRjJ|M{C3O|UzRC:jPh?=
HT-22  MmnyR4VtdCCYaXHibYxqfHliQYPzZZk> MVuxNOKh|ryP M2DhfVEzyqCq MYDEUXNQ MXfwdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIHPlcIwh\GWjdHi= MofjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MEe3OVIoRjJ|M{C3O|UzRC:jPh?=
KMS-12-BM Mkf4R5l1d3SxeHnjbZR6KEG|c3H5 Ml7QPVAhyrWP MYWxJIg> MYjicI9kc3NiQlHZJFEyNTdyOEKgbY5lfWOnZDDyZZBq\CClZXzsJJN4\WyuaX7n NFXScHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyO|E2PCd-MkO1NlcyPTR:L3G+
MM.1S  M2C3WmN6fG:2b4jpZ4l1gSCDc4PhfS=> NX\2PVJMQTBiwsXN MYexJIg> NWe1UYs3[myxY3vzJGJCYSBzMT23NFgzKGmwZIXj[YQhemGyaXSgZ4VtdCC|d3XscIlv\w>? MojtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkexOVQoRjJ|NUK3NVU1RC:jPh?=
ΔN-Karpas 299  NX7CXIpIS3m2b4TvfIlkcXS7IFHzd4F6 MVGyNOKh|ryP M3voWVE3KGh? MVfpcohq[mm2czDDSFMxNWmwZIXj[YQh[2WubDDk[YF1cA>? NUnreYZyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFU6OzhpPkKzOVQ2QTN6PD;hQi=>
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RMS13 MVLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M4D5[|Qx6oDLzsznM41tyqB? NIf0OpgzPCCq M4C5NpJme2O3ZYOgS3gyPS1yN{CtbY5lfWOnZDDsc5N{KG:oIHPlcIwhfmmjYnnsbZR6 NGXXdFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e0OFI6Pid-MkO3OFQzQTZ:L3G+
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Jurkat MVTD[YxtKGSnYYToJIF{e2G7 NEGzNZI1QCCqcoO= NG\TVWFKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxteyCmZX\pZ4lmdnRiaX6gSmFFTCCjbnSg[ZhxemW|c3nu[{BTUVBia3nuZZNmKGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHnuJJBz\XOnbnPlJI9nKEGSMUWxNEwhgl[DRD3mcYs> M{HKNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
Jurkat NFvYdmZE\WyuIHTlZZRpKGG|c3H5 M1v6VFQ5KGi{cx?= NET2SmVKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxteyCmZX\pZ4lmdnRiaX6gSmFFTCCjbnSg[ZhxemW|c3nu[{BTUVBiS{S1UUBufXSjboSgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF\BR3MhcW5icILld4Vv[2Vib3[gRXAyPTFyLDD6WmFFNW[vax?= NX[0dph5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat NFrIb2NE\WyuIHTlZZRpKGG|c3H5 NX\XZlE5PDhiaILz M3rJSWlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGSnZnnjbYVvfCCrbjDGRWRFKGGwZDDlfJBz\XO|aX7nJHJKWCCtaX7hd4Uh\G:vYXnuJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGmwIIDy[ZNmdmOnIH;mJGFROTVzMDygfnZCTC2obXu= NFrYdYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat M2nQW2NmdGxiZHXheIgh[XO|YYm= NV\XdHlwPDhiaILz MU\Jcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDGT2JROTJvYnHz[YQh\GmvZYLpfoF1cW:wIHTvcYFqdiCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhTkGFUzDpckBxemW|ZX7j[UBw\iCDUEG1NVA> MlT3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat MUjD[YxtKGSnYYToJIF{e2G7 NYTGcoloPDhiaILz NFy3WnJKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxteyCmZX\pZ4lmdnRiaX6gSmFFTCCjbnSg[ZhxemW|c3nu[{BTUVBia3nuZZNmKGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHnuJJBz\XOnbnPlJI9nKEGSMUWxNC=> NFO0WpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat MVzD[YxtKGSnYYToJIF{e2G7 NGjH[3k1QCCqcoO= MlT4TY5pcWKrdHnvckBw\iCmZXH0bEBz\WOncITvdkB{cWewYXzpcochdWWmaXH0[YQhdmWlcn;weI91cWNiY3XscEBl\WG2aDDpckBpfW2jbjDKeZJs[XRiY3XscJMh\GWoaXPp[Y51KGmwIF\BSGQh[W6mIHX4dJJme3OrbnegVmlRKEt2NV2gcZV1[W62IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCIQVPTJIlvKHC{ZYPlcoNmKG:oIFHQNVUyOA>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Jurkat M4jmdGNmdGxiZHXheIgh[XO|YYm= MUi0PEBpenN? NEnWUotKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxteyCmZX\pZ4lmdnRiaX6gSmFFTCCjbnSg[ZhxemW|c3nu[{BTUVBia3nuZZNmKGSxbXHpckBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCrbjDwdoV{\W6lZTDv[kBCWDF3MUC= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Sf9 NYj5NYtDTnWwY4Tpc44h[XO|YYm= MnfUTY5pcWKrdHnvckBw\iCqdX3hckBTUVBzIFu0OW0hdXW2YX70JIF2fG:yaH;zdIhwenmuYYTpc44h\XiycnXzd4VlKGmwIGPmPUBk\Wyucx?= M1T2[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEC4O|E{Lz5zOESwPFcyOzxxYU6=
Jurkat Mn7yR5l1d3C{b4TlZ5RqfmViYYPzZZk> MkjTN|AhfU1? MmHLNUBpeg>? MVnDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KE[jc1ytbY5lfWOnZDDu[YNzd3C2b4Ppd{BqdiCqdX3hckBLfXKtYYSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZZQhOzBidV2gbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KE[jc1ygd5RqdXWuYYTpc44hdWWjc4Xy[YQh[W[2ZYKgNlAhcHK|IHL5JGFt[W2jcjDicJVmKGG|c3H5 M13xW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|U4Lz5{OUW0NVM2PzxxYU6=
Jurkat MoCxR5l1d3C{b4TlZ5RqfmViYYPzZZk> MUezNEB2VQ>? MmfPNUBpeg>? NFTjZXlEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFPIXE1qdmS3Y3XkJI5m[3KxcITvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCB|MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliQ1jYJJN1cW23bHH0bY9vKGK7IFHsZY1ieiCkbIXlJIF{e2G7 MnvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzOVcoRjJ7NUSxN|U4RC:jPh?=
Jurkat Ml:1R5l1d3C{b4TlZ5RqfmViYYPzZZk> MmXFN|AhfU1? Mlj6NUBpeg>? Ml3CR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCcLW\BSE1qdmS3Y3XkJI5m[3KxcITvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCB|MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliWj3WRWQhe3SrbYXsZZRqd25iYomgRYxidWG{IHLseYUh[XO|YYm= NX7Bd3FZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PTdpPkK5OVQyOzV5PD;hQi=>
Jurkat Mke0R5l1d3C{b4TlZ5RqfmViYYPzZZk> M1nZcVMxKHWP M3PXNlEhcHJ? MXnDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KE[jc1ytbY5lfWOnZDDu[YNzd3C2b4Ppd{BqdiCqdX3hckBLfXKtYYSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZZQhOzBidV2gbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KE[jc1ygd5RqdXWuYYTpc44hdWWjc4Xy[YQh[W[2ZYKgNlAhcHK|IHL5JJBp[XOnIHPvcpRz[XO2IH3pZ5Jwe2OxcIm= Mn;wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzOVcoRjJ7NUSxN|U4RC:jPh?=
Jurkat M{i4dmN6fG:ycn;0[YN1cX[nIHHzd4F6 MXmzNEB2VQ>? NV3OdWdLOSCqch?= NG[4VWxEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFPIXE1qdmS3Y3XkJI5m[3KxcITvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCB|MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliQ1jYJJN1cW23bHH0bY9vKGK7IIDoZZNmKGOxboTyZZN1KG2rY4Lvd4NweHl? NVPRRVlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PTdpPkK5OVQyOzV5PD;hQi=>
Jurkat M3flcGN6fG:ycn;0[YN1cX[nIHHzd4F6 NXPSWWhDOzBidV2= MonkNUBpeg>? MlLmR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCcLW\BSE1qdmS3Y3XkJI5m[3KxcITvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCB|MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliWj3WRWQhe3SrbYXsZZRqd25iYomgdIhie2ViY3;ueJJie3RibXnjdo9{[2:yeR?= NV2xNXVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PTdpPkK5OVQyOzV5PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Immunofluorescence
RIP1 / RIP3 ; 

PubMed: 30462730     


Immunofluorscence data showed an increased expression of RIP1 and RIP3 in Pg-LPS group and an eminent reduction of RIP1 in the Pg-LPS+Nec-1 group.

30462730

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

RIP1 激酶检测:

RIP1 的磷酸化需要其激酶活性。FLAG标记的野生型(WT)或RIP1(K45M) 突变体失活激酶的表达结构转染到293T细胞中,在有[γ-32P]ATP存在时,RIP1激酶实验在30°C下进行30分钟。样品进行SDS-PAGE,通过放射自显影可观察到RIP1带。对放射性带的相对强度进行量化,并显示比率。在激酶反应的同时,珠样本使用anti-RIP1抗体进行Western Blot分析,确保与激酶反应中等量的蛋白。
细胞实验:

[2]

- 合并
  • Cell lines: Jurkat, BALB/c 3T3, SV40-转化的MEF, L929
  • Concentrations: 0.01-100 μM
  • Incubation Time: --
  • Method:

    细胞接种在96孔板中(白色板进行发光检测;黑色板进行荧光检测;空白板进行MTT实验)贴壁细胞按每孔5000-10000个细胞的密度接种,悬浮细胞按每孔20,000-50,000个细胞的密度接种,孔中含100 μl合适的无酚红培养基。温育后,使用如下方法之一测定细胞存活率。ATP 实验中,使用购买的发光试剂盒,并使用Wallac Victor II酶标仪分析发光值。Sytox实验中,细胞与 1 μM Sytox Green试剂在37°C下温育30分钟,然后进行荧光读数。随后,增加5 μl 20% Triton X-100溶液到每孔中,产生最大溶解,细胞37°C下温育1小时,然后进行二次读数。Triton处理前和后,计算值的比率。MTT实验,使用CellTiter 96 AQueous 非放射性细胞增殖检测试剂盒。PI排除实验中, 加入2 μg/ml PI 到培养基中,立即使用FACSCalibur分析样品。PI-膜联蛋白V 实验中,使用ApoAlert Annexin V-EGFP 凋亡试剂盒。进行DioC6染色, 细胞与40 nM DiOC6 在37°C下温育30分钟, 洗涤一次,使用FACSCalibur分析。ROS分析中, 细胞与5 μM Dihydroethidium在37°C下温育30分钟, 洗涤一次,使用FACSCalibur分析。


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 51 mg/mL warmed (196.66 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+45% PEG 300+ddH2O
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 259.33
化学式

C13H13N3OS

CAS号 4311-88-0
储存条件 粉状
溶于溶剂
别名 Nec-1

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID