Necrostatin-1

目录号:S8037 别名: Nec-1

仅限科研使用

Necrostatin-1 (Nec-1)是一种特异性RIP1 (RIPK1)抑制剂,抑制TNF-α诱导的细胞坏死,在293T细胞中EC50为490 nM。Necrostatin-1也可抑制 IDO、细胞自噬和凋亡。

Necrostatin-1 Chemical Structure

CAS: 4311-88-0

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1054.09 现货
RMB 814.14 现货
RMB 2224.79 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

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客户使用Selleck生产的Necrostatin-1发表文献142篇:

产品安全说明书

RIP kinase抑制剂选择性比较

生物活性

产品描述 Necrostatin-1 (Nec-1)是一种特异性RIP1 (RIPK1)抑制剂,抑制TNF-α诱导的细胞坏死,在293T细胞中EC50为490 nM。Necrostatin-1也可抑制 IDO、细胞自噬和凋亡。
特性 Necrostatin-1是抑制细胞坏死的有力工具。
靶点
RIP1 [1]
(293T cells)
490 nM(EC50)
体外研究

Necrostatin-1 (1-100 μM) 抑制过表达和内源性的RIP1发生自磷酸化。RIP1是初级细胞靶点,负责Necrostatin-1的抗细胞坏死活性。[1] Necrostatin-1有效抑制多种类型细胞触发的坏死性细胞死亡。Necrostatin-1作为细胞坏死的小分子抑制剂, 作用于jurkat细胞,抑制RIP激酶的诱导细胞坏死,抑制TNF-α诱导的细胞坏死,EC50为490 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-22 NV\kS3ZvS3m2b4TvfIlkcXS7IFHzd4F6 MWGxNEDPxE1? MmXaNVIhcA>? M2K3UZBzd3SnY4TzJIFo[Wmwc4SgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDYEoH3tc4wwVCCpbIX0ZY1ifGYEoB?= NFLMUWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{e2NFg3QSd-MUe3OlA5Pjl:L3G+
Jurkat  MmfPSpVv[3Srb36gRZN{[Xl? NHrtb3ozODEEoN88cS=> M1radVMxKG2rbh?= MnPadoVlfWOnc9MgUoFm\2yncnnhJIZwf2yncnmtbY5lfWOnZDDy[YFkfGm4ZTDvfJlo\W5ic4DlZ4lmeyBqUl;TLUBo\W6ncnH0bY9v NEXuNlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWzOVAzOCd-MkG1N|UxOjB:L3G+
Jurkat  Mk\yR5l1d3SxeHnjbZR6KEG|c3H5 NWDMO4JwPTBxIEGwNE8zODEEoN88cS=> NUTqVVQzOS9|IHi= MXjy[YR2[2W|wrDOZYVodGW{aXGg[o94dGW{aT3pcoR2[2WmIHP5eI91d3irY3n0fS=> M1z1XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUO1NFIxLz5{MUWzOVAzODxxYU6=
SW13 NH\wSJlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MXexNFAh|ryP NVXYe2FnOjRiaB?= NF\YclRFVVOR MV7pcoNz\WG|ZYOgZ4VtdHWuYYKgd5Vzfmm4YXy= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF|NkixPEc,OjJzM{[4NVg9N2F-
8505c NWT6TolJS2WubDDWbYFjcWyrdImgRZN{[Xl? NIfncHcyODBizszN NY\OeZY4OjRiaB?= M{HjTmROW09? NVuzZYZrcW6lcnXhd4V{KGOnbHz1cIFzKHO3co\peoFt NE\GcYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzOlgyQCd-MkKxN|Y5OTh:L3G+
TPC-1 NFn6cYNE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NGLTeooyODBizszN MYSyOEBp MYjEUXNQ NHLaUGJqdmO{ZXHz[ZMh[2WubIXsZZIhe3W{dnn2ZYw> NH3ic3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzOlgyQCd-MkKxN|Y5OTh:L3G+
L929sA MnPLRZBweHSxc3nzJGF{e2G7 Mnv0NVAh|ryP NWDzWmFFOSCq NHizbGZi[nKxZ3H0[ZMhfGinIHnueIVz[WO2aX;uJI9nKGOjc4Dhd4UuQCC5aYToJGZCTER? MlrkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NkK3OlcoRjJ{M{[yO|Y4RC:jPh?=
L929sA MVfBdI9xfG:|aYOgRZN{[Xl? MUixNEDPxE1? NH74Z2wyKGh? MlzadoV{[3WnczDj[YxteyCneIDy[ZN{cW6pIGLJVGsy|pSLRDDmdo9uKFSQRj3pcoR2[2WmIHHwc5B1d3Orcx?= M3LjblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{[yO|Y4Lz5{MkO2Nlc3PzxxYU6=
L929sA NH\rR4JCeG:ydH;zbZMhSXO|YYm= NHLzSIgyOCEQvF2= MmXONUBp MX;pcohq[mm2czD0bIUh[XCxcITveIlkKHKnc4DvcpNmKHSxIGTOSi=> MkDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NkK3OlcoRjJ{M{[yO|Y4RC:jPh?=
K562/Adr  NH6wV|VHfW6ldHnvckBCe3OjeR?= NV3Z[pdPPjBizszN MYGxNkBp NEThb29qdmO{ZXHz[ZMhfGinIHHjeIl3cXS7IH;mJINie3Cjc3XzMEBk[XOyYYPlJFgh[W6mIEm= NWDZS44yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
K562 M{DwOmZ2dmO2aX;uJGF{e2G7 NYjiWWoxPjBizszN MlKyNVIhcA>? M37iT4lv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= NInnUm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
HL60/Adr NFzzZo5HfW6ldHnvckBCe3OjeR?= NWHITYlnPjBizszN MWWxNkBp M3z6PYlv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|N{[4PUc,OjJ6M{e2PFk9N2F-
HL60 M2TMOGZ2dmO2aX;uJGF{e2G7 M{e1bFYxKM7:TR?= MonINVIhcA>? MYTpcoNz\WG|ZYOgeIhmKGGldHn2bZR6KG:oIHPhd5Bie2W|LDDjZZNx[XOnIEigZY5lKDl? NYjzWVF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
K562/Adr  MnyySpVv[3Srb36gRZN{[Xl? NV3kboZJPjBizszN MXWxNkBp M13TTIF2\22nboTzJJRp\SClYYPwZZNmNTNiYXP0bZZqfHl? MnjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
K562 NYPsbYdETnWwY4Tpc44hSXO|YYm= MkL2OlAh|ryP MUOxNkBp M4ribIF2\22nboTzJJRp\SClYYPwZZNmNTNiYXP0bZZqfHl? M{GzXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60/Adr NEPNdXBHfW6ldHnvckBCe3OjeR?= NHrmboc3OCEQvF2= MUSxNkBp MUXheYdu\W62czD0bIUh[2G|cHHz[U0{KGGldHn2bZR6 M3nwelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60 NVzEWXRqTnWwY4Tpc44hSXO|YYm= NFq2SXM3OCEQvF2= M2DJTVEzKGh? NGHEPHVifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 MojEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
K562/Adr  MVvBdI9xfG:|aYOgRZN{[Xl? M3;BOFYxKM7:TR?= MnXkNVIhcA>? NWHT[3Z3\W6qYX7j[ZMhe2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZM> NI\HOXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
K562 MoPjRZBweHSxc3nzJGF{e2G7 MmixOlAh|ryP Mn\PNVIhcA>? Mmrj[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|N{[4PUc,OjJ6M{e2PFk9N2F-
HL60/Adr MWfBdI9xfG:|aYOgRZN{[Xl? NGqwXI03OCEQvF2= M{LOc|EzKGh? NYXiN21E\W6qYX7j[ZMhe2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZM> MkO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
HL60 M1HuO2Fxd3C2b4Ppd{BCe3OjeR?= M2XOZlYxKM7:TR?= NHrWcXUyOiCq Mkfl[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= NX;1VFhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
SH-EP NGTFeXZCeG:ydH;zbZMhSXO|YYm= MW[xNQKBkc7:TdMg MmTPO|LjiImqwrC= NHezNldqdmirYnn0d{BKSVBiaX7obYJqfG:{LTDhcoQhVGW6YYT1cZVu[WJvaX7keYNm\CCjcH;weI9{cXN? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh7MEOyNkc,OjJ6OUCzNlI9N2F-
NIH3T3  MmDLSpVv[3Srb36gRZN{[Xl? MnjRNVAwPTBizszN NXjQ[pJKOS9|IHi= M1zyUoFu\Wyrb4LheIV{KFSQRt8xMYRzcX[nbjDjc41xdGW6IH\vdo1ifGmxbh?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4MU[3O{c,OjN{NkG2O|c9N2F-
HT-22  MUnGeY5kfGmxbjDBd5NigQ>? MoCyNlXDqM7:TR?= NX\OSYVNOOLCk{OwxsBucW5? NXTmT2RJTE2VTx?= M1yzWYlvcGmkaYTzJGVTUyCDY4TpeoF1cW:wIHnu[JVk\WRiYomg[4x2fGGvYYTl NYTYXVJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFc4PTJpPkKzN|A4PzV{PD;hQi=>
HT-22  MXTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NXm1TYR6OTEEoN88US=> NHr4TlUyOsLiaB?= NUHpbZdvTE2VTx?= MV\wdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIHPlcIwh\GWjdHi= NVr4[5F7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFc4PTJpPkKzN|A4PzV{PD;hQi=>
KMS-12-BM M4nPd2N6fG:2b4jpZ4l1gSCDc4PhfS=> M{nXWFkxKML3TR?= MV6xJIg> M1z1OIJtd2OtczDCRXkhOTFvN{C4NkBqdmS3Y3XkJJJieGmmIHPlcIwhe3enbHzpcoc> NE\y[VU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyO|E2PCd-MkO1NlcyPTR:L3G+
MM.1S  MnToR5l1d3SxeHnjbZR6KEG|c3H5 NXTBc3VwQTBiwsXN MV[xJIg> MkHYZoxw[2u|IFLBXUAyOS15MEiyJIlv\HWlZXSgdoFxcWRiY3XscEB{f2WubHnu[y=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV{N{G1OEc,OjN3MkexOVQ9N2F-
ΔN-Karpas 299  MX;DfZRwfG:6aXPpeJkhSXO|YYm= MVOyNOKh|ryP MkK2NVYhcA>? MX7pcohq[mm2czDDSFMxNWmwZIXj[YQh[2WubDDk[YF1cA>? MmDLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEW5N|goRjJ|NUS1PVM5RC:jPh?=
MEFs NEXDfoJHfW6ldHnvckBCe3OjeR?= MmfiNlDDqM7:TR?= M2fRNVEwOi92IHi= M2L3[5N2eHC{ZYPz[ZMhXE6IzsGtbY5lfWOnZDDSTXBMOSCyaH;zdIhwenmuYYTpc44> Mo\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5Mke1PFEoRjJ|N{K3OVgyRC:jPh?=
MEFs NHfGb2VEgXSxdH;4bYNqfHliQYPzZZk> M1\r[lIwPi9{MDFOwG0> NUXXOHhJOThiaB?= NHzrZXBqdmirYnn0d{BVVkcQsT3pcoR2[2WmIHPlcIwh\GWjdHigbY4hWmWuQTDLU{BOTU[| NFLBO|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{eyO|U5OSd-MkO3Nlc2QDF:L3G+
RMS13 MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnX5OFDjiIoQvHevcYzDqA>? Ml\QNlQhcA>? M4XScJJme2O3ZYOgS3gyPS1yN{CtbY5lfWOnZDDsc5N{KG:oIHPlcIwhfmmjYnnsbZR6 M{nqfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{S0Nlk3Lz5{M{e0OFI6PjxxYU6=
TE671 M2PuTWNmdGxiVnnhZoltcXS7IFHzd4F6 NWfZ[IhCPDEkgJpOwIcwdW{EoB?= MYOyOEBp NV30SVlzemW|Y4Xld{BIYDF3LUC3NE1qdmS3Y3XkJIxwe3Nib3[gZ4VtdCC4aXHibYxqfHl? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2NEK5Okc,OjN5NESyPVY9N2F-
U87 NGnpdnRHfW6ldHnvckBCe3OjeR?= NFu0d2wyKG2vb3yvUC=> MWixMlUuOyCq NGTwd49{fXCycnXzd4V{KHSqZTDlfJBz\XO|aX;uJI9nKFKLUD2xJINifXOnZDDifUB{cGmtb37pci=> NHG3PVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0NFQ1OSd-MkO4OFA1PDF:L3G+
C6 NVrCcpplTnWwY4Tpc44hSXO|YYm= NIT6OoIyKG2vb3yvUC=> Mm\DNU42NTNiaB?= MnTDd5VxeHKnc4Pld{B1cGViZYjwdoV{e2mxbjDv[kBTUVBvMTDjZZV{\WRiYomgd4hqc2:waX6= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2MES0NUc,OjN6NEC0OFE9N2F-
U87 MWrDfZRwfG:6aXPpeJkhSXO|YYm= NFjOO3kyKG2vb3yvUC=> NHqz[Hc{KGh? M1LHRYJtd2OtczDzbIlsd26rbjDpcoR2[2WmIH7lZ5Jwe2m| Ml;LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEC0OFEoRjJ|OESwOFQyRC:jPh?=
C6 Ml\kR5l1d3SxeHnjbZR6KEG|c3H5 NEjhe2IyKG2vb3yvUC=> NYX0V3puOyCq M1yye4Jtd2OtczDzbIlsd26rbjDpcoR2[2WmIH7lZ5Jwe2m| MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2MES0NUc,OjN6NEC0OFE9N2F-
U87 M3nSdmNmdGxiVnnhZoltcXS7IFHzd4F6 MojrNUBudW:uL1y= M1XHN|MhcA>? NF\kXlFifHSnboXheIV{KFOqaXvvcolvKGmwZIXj[YQh\2yrb33hJINmdGxiZHXheIg> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2MES0NUc,OjN6NEC0OFE9N2F-
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Jurkat M1vSVmNmdGxiZHXheIgh[XO|YYm= M1f3NVQ5KGi{cx?= M{HjeWlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGSnZnnjbYVvfCCrbjDGRWRFKGGwZDDlfJBz\XO|aX7nJHJKWCCNNEXNJI12fGGwdDDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliRlHDV{BqdiCycnXz[Y5k\SCxZjDBVFE2OTBuIIrWRWQu\m2t NHnZe289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
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Jurkat NIrXR5BE\WyuIHTlZZRpKGG|c3H5 NXnUcGFHPDhiaILz MV7Jcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDGT2JROTJvYnHz[YQh\GmvZYLpfoF1cW:wIHTvcYFqdiCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhTkGFUzDpckBxemW|ZX7j[UBw\iCDUEG1NVA> M{D3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
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Jurkat NXrzZmtrS2WubDDk[YF1cCCjc4PhfS=> NHvuV481QCCqcoO= NEfBRWtKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxteyCmZX\pZ4lmdnRiaX6gSmFFTCCjbnSg[ZhxemW|c3nu[{BTUVBiS{S1UUBufXSjboSgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF\BR3MhcW5icILld4Vv[2Vib3[gRXAyPTFy MlXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat NWCzNWtbS2WubDDk[YF1cCCjc4PhfS=> MmfaOFghcHK| MU\Jcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDSTXAhc2mwYYPlJIRwdWGrbjDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliRlHDV{BqdiCycnXz[Y5k\SCxZjDBVFE2OTB? NGDPS5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Sf9 MoTJSpVv[3Srb36gZZN{[Xl? MoCyTY5pcWKrdHnvckBw\iCqdX3hckBTUVBzIFu0OW0hdXW2YX70JIF2fG:yaH;zdIhwenmuYYTpc44h\XiycnXzd4VlKGmwIGPmPUBk\Wyucx?= M3foSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEC4O|E{Lz5zOESwPFcyOzxxYU6=
Jurkat M2K4cGN6fG:ycn;0[YN1cX[nIHHzd4F6 NUjwOWk2OzBidV2= MUexJIhz MofZR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCIYYPMMYlv\HWlZXSgcoVkem:ydH;zbZMhcW5iaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIF1KDNyIIXNJIlv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCIYYPMJJN1cW23bHH0bY9vKG2nYYP1doVlKGGodHXyJFIxKGi{czDifUBCdGGvYYKgZox2\SCjc4PhfS=> NFnqc|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM2Pyd-Mkm1OFE{PTd:L3G+
Jurkat NF3DZ4ZEgXSxcILveIVkfGm4ZTDhd5NigQ>? MoTvN|AhfU1? NIXlRnQyKGi{ M4jhZWN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiQ1jYMYlv\HWlZXSgcoVkem:ydH;zbZMhcW5iaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIF1KDNyIIXNJIlv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCFSGigd5RqdXWuYYTpc44h[nliQXzhcYFzKGKudXWgZZN{[Xl? NVzQblBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PTdpPkK5OVQyOzV5PD;hQi=>
Jurkat NIK2cWJEgXSxcILveIVkfGm4ZTDhd5NigQ>? NUPBS4FqOzBidV2= NUezN3puOSCqch?= NETjSW1EgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IGqtWmFFNWmwZIXj[YQhdmWlcn;weI9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGmwY4XiZZRm\CCob4KgNUBpeiCob3zsc5dm\CCkeTDaMXZCTCC|dHnteYxifGmxbjDifUBCdGGvYYKgZox2\SCjc4PhfS=> M1fxblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|U4Lz5{OUW0NVM2PzxxYU6=
Jurkat NXywdHhES3m2b4Dyc5Rm[3SrdnWgZZN{[Xl? NGXBfpY{OCC3TR?= NG[5dVEyKGi{ NYDkW2d4S3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDGZZNNNWmwZIXj[YQhdmWlcn;weI9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGmwY4XiZZRm\CCob4KgNUBpeiCob3zsc5dm\CCkeTDGZZNNKHO2aX31cIF1cW:wIH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgSCyaHHz[UBkd262cnHzeEBucWO{b4Pjc5B6 MnvLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzOVcoRjJ7NUSxN|U4RC:jPh?=
Jurkat NGDGPIFEgXSxcILveIVkfGm4ZTDhd5NigQ>? NH\YZmY{OCC3TR?= M1TQOVEhcHJ? MoOzR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCFSGitbY5lfWOnZDDu[YNzd3C2b4Ppd{BqdiCqdX3hckBLfXKtYYSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZZQhOzBidV2gbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KEOKWDDzeIlufWyjdHnvckBjgSCyaHHz[UBkd262cnHzeEBucWO{b4Pjc5B6 M{\ufVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|U4Lz5{OUW0NVM2PzxxYU6=
Jurkat MXHDfZRweHKxdHXjeIl3\SCjc4PhfS=> NHT2e5A{OCC3TR?= M1PaVlEhcHJ? MV\DfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KFpvVlHEMYlv\HWlZXSgcoVkem:ydH;zbZMhcW5iaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIF1KDNyIIXNJIlv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCcLW\BSEB{fGmvdXzheIlwdiCkeTDwbIF{\SClb370doF{fCCvaXPyc5Nkd3C7 NFfCUHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM2Pyd-Mkm1OFE{PTd:L3G+
Assay
Methods Test Index PMID
Immunofluorescence RIP1 / RIP3 30462730

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

  • RIP1 激酶检测:

    RIP1 的磷酸化需要其激酶活性。FLAG标记的野生型(WT)或RIP1(K45M) 突变体失活激酶的表达结构转染到293T细胞中,在有[γ-32P]ATP存在时,RIP1激酶实验在30°C下进行30分钟。样品进行SDS-PAGE,通过放射自显影可观察到RIP1带。对放射性带的相对强度进行量化,并显示比率。在激酶反应的同时,珠样本使用anti-RIP1抗体进行Western Blot分析,确保与激酶反应中等量的蛋白。

细胞实验:

[2]

  • Cell lines: Jurkat, BALB/c 3T3, SV40-转化的MEF, L929
  • Concentrations: 0.01-100 μM
  • Incubation Time: --
  • Method:

    细胞接种在96孔板中(白色板进行发光检测;黑色板进行荧光检测;空白板进行MTT实验)贴壁细胞按每孔5000-10000个细胞的密度接种,悬浮细胞按每孔20,000-50,000个细胞的密度接种,孔中含100 μl合适的无酚红培养基。温育后,使用如下方法之一测定细胞存活率。ATP 实验中,使用购买的发光试剂盒,并使用Wallac Victor II酶标仪分析发光值。Sytox实验中,细胞与 1 μM Sytox Green试剂在37°C下温育30分钟,然后进行荧光读数。随后,增加5 μl 20% Triton X-100溶液到每孔中,产生最大溶解,细胞37°C下温育1小时,然后进行二次读数。Triton处理前和后,计算值的比率。MTT实验,使用CellTiter 96 AQueous 非放射性细胞增殖检测试剂盒。PI排除实验中, 加入2 μg/ml PI 到培养基中,立即使用FACSCalibur分析样品。PI-膜联蛋白V 实验中,使用ApoAlert Annexin V-EGFP 凋亡试剂盒。进行DioC6染色, 细胞与40 nM DiOC6 在37°C下温育30分钟, 洗涤一次,使用FACSCalibur分析。ROS分析中, 细胞与5 μM Dihydroethidium在37°C下温育30分钟, 洗涤一次,使用FACSCalibur分析。

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+45% PEG 300+ddH2O

10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 259.33
化学式

C13H13N3OS

CAS号 4311-88-0
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

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