Avelumab (anti-PD-L1)

目录号:A2015 别名: Bavencio, MSB0010718C 中文名称:阿维单抗

For research use only.
Not for use in humans.

Avelumab (anti-PD-L1) (Bavencio, MSB0010718C) 是一种完全人IgG1单克隆抗体,靶向 protein programmed death-ligand 1 (PD-L1)。Avelumab 具有潜在的抗体依赖性细胞介导的细胞毒性作用,可用于多种癌症的治疗。MW=143.8 kDa。

规格 价格 库存 购买数量  
RMB 5807.53 现货
RMB 17420.87 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

质量管理及产品安全说明书

PD-1/PD-L1抑制剂选择性比较

生物活性

产品描述 Avelumab (anti-PD-L1) (Bavencio, MSB0010718C) 是一种完全人IgG1单克隆抗体,靶向 protein programmed death-ligand 1 (PD-L1)。Avelumab 具有潜在的抗体依赖性细胞介导的细胞毒性作用,可用于多种癌症的治疗。MW=143.8 kDa。
体外研究

Co-incubating chordoma cells with brachyury-specific CD8+ T cells results in significant upregulation of PD-L1 on the tumor cells, mediated by the CD8+ T cells' IFN-γ production, and increases sensitivity of chordoma cells to avelumab-mediated ADCC. Residential cancer stem cell subpopulations of chordoma cells are also killed by avelumab-mediated ADCC to the same degree as non-cancer stem cell populations. As a monotherapy for chordoma, avelumab may enable endogenous NK cells, while in combination with T-cell immunotherapy, such as a vaccine, avelumab may enhance NK-cell killing of chordoma cells via ADCC.[1]

体内研究

An aggressive, bioluminescent orthotopic bladder cancer model, MB49 tumor cells transfected with luciferase (MB49luc) is used to study the antitumor effects of avelumab. MB49luc bladder tumors are highly positive for the expression of PD-L1 and avelumab administration induces significant (P<0.05) antitumor effects.[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: Antibody-dependent cellular cytotoxicity assay
    Cells: UM-Chor1 cells
    Concentrations: 2 μg/mL
    Incubation Time: 30 min
    Method: To examine the relationship between a CSC subpopulation and ADCC activity, UM-Chor1 cells are left untreated or treated with 50 ng/mL of IFN-γ for 24 h. Cells are then plated as targets at 50,000 cells/well in 6-well round-bottom culture plates and incubated with 2 μg/mL of avelumab at room temperature for 30 min. NK cells are added at 2500,000 cells/well at an E:T ratio of 50:1. After 4 h, tumor cells are harvested and stained with antibodies for flow cytometry.
    Reference: https://pubmed.ncbi.nlm.nih.gov/27172898

    Avelumab can apply to humanized mice, non-humanized mice (eg: C57BL/6 mice), peripheral blood and other related assays (Only for Reference)

动物实验:
- 合并
  • Objective: Measurement of individual tumors
    Animal Models: 16- to 18-week-old female C57BL/6 mice
    Formulation: DPBS
    Dosages: 400 μg/100 μL
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/26921031/

    Avelumab can apply to humanized mice, non-humanized mice (eg: C57BL/6 mice), peripheral blood and other related assays (Only for Reference)

抗体信息

CAS No. 1537032-82-8
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 Human
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

技术支持

如果有其他问题,请给我们留言。

  • * 必填项
Tags: 购买Avelumab (anti-PD-L1) | Avelumab (anti-PD-L1)供应商 | 采购Avelumab (anti-PD-L1) | Avelumab (anti-PD-L1)价格 | Avelumab (anti-PD-L1)生产 | 订购Avelumab (anti-PD-L1) | Avelumab (anti-PD-L1)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID