LY2584702

For research use only. Not for use in humans.

目录号:S7698

LY2584702 Chemical Structure

CAS No. 1082949-67-4

LY2584702是一种选择性的,ATP竞争性的p70S6K抑制剂,IC50为4 nM。Phase 1。

规格 价格 库存 购买数量  
RMB 1218.71 现货
RMB 3647.46 现货
RMB 8001.67 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的LY2584702发表文献10篇:

客户使用该产品的5个实验数据:

  • Murine DCs were pretreated with PBS or kinase inhibitors (10 μmol/l) wortmanni LY2584702 2 h prior to nicotine (10−7 mol/l) 12~15 h stimulation. MR expression was determined via western blot analyses (C, F). β-actin was used as an internal control.

    Oncotarget, 2016, 7(25):38451-38466. LY2584702 purchased from Selleck.

    (A) Flow cytometry for TNF-α and IL-12 p40 intracellular expression and ELISA assays for IL-12p70 and IL-23 in supernatants of untreated or treated cells after 24 h. Results were expressed as pictograms per milliliter (± SD) from four independent experiments, each performed in triplicate. *p < 0.05 versus IFN-γ, **p < 0.01 versus IFN-γ.

    J Immunol, 2016, 197(9):3545-3553. LY2584702 purchased from Selleck.

  • Peripheral blood monocytes were mock or HCMV infected, or treated with GMCSF or M-CSF for 30 min. Levels of p-S6K (T389), and S6K were detected by immunoblotting from whole cell lysates. Membranes were then reprobed for β-actin as a loading control. Data are representative of 3-6 independent blood donors.

    Antiviral Res, 2018, 158:13-24. LY2584702 purchased from Selleck.

    Pre-warmed human neutrophil suspensions (37°C, 5 million cells/ml in HBSS containing 1.6 mM CaCl2) were incubated with PF-4708671, LY2584702 or vehicle (DMSO) for 5 minutes, then stimulated with 100 nM thapsigargin for 10 minutes. B) Samples were processed and analyzed for S6 and phospho-S6 content as described in methods. Data are from one experiment, representative of three.

    PLoS One, 2017, 12(1):e0169804. LY2584702 purchased from Selleck.

  • Western blot analysis of RPS6KB1, p-RPS6KB1, rpS6 and p-rpS6 in adenocarcinoma cell line A549 and squamous cell carcinoma cell line SK-MES-1 with various LY2584702 concentrations. DMSO without any LY2584702 were expressed as a concentration of 0. β-actin was used as a loading control.

    PLoS One, 2017, 12(8):e0182891. LY2584702 purchased from Selleck.

产品安全说明书

S6 Kinase抑制剂选择性比较

生物活性

产品描述 LY2584702是一种选择性的,ATP竞争性的p70S6K抑制剂,IC50为4 nM。Phase 1。
靶点
p70S6K [1]
4 nM
体外研究

在HCT116结肠癌细胞,LY2584702抑制S6核糖体蛋白质(pS6)的磷酸化,IC50 为0.1-0.24 μM。[1] LY258470与EGRF抑制剂erlotinib或mTOR抑制剂everolimus结合时,具有显著的协同效应。[2]

Assay
Methods Test Index PMID
Western blot
p-rpS6 / rpS6 / p-RPS6KB1 / RPS6KB1 ; 

PubMed: 28792981     


Western blot analysis of RPS6KB1, p-RPS6KB1, rpS6 and p-rpS6 in adenocarcinoma cell line A549 and squamous cell carcinoma cell line SK-MES-1 with various LY2584702 concentrations. DMSO without any LY2584702 were expressed as a concentration of 0. β-actin was used as a loading control.

28792981
体内研究 LY2584702 (12.5 mg/kg BID),在U87MG 成胶质细胞瘤和HCT116结肠癌异种移植物模型中表现出显著的抗肿瘤作用。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:

[1]

- 合并
  • Animal Models: U87MG 恶性胶质瘤和 HCT116 结肠癌异种移植物模型
  • Dosages: 12.5 mg/kg BID
  • Administration: --
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 1 mg/mL warmed (2.24 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 445.42
化学式

C21H19F4N7

CAS号 1082949-67-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01241461 Completed Drug: LY2584702 Cancer Eli Lilly and Company November 2010 Phase 1
NCT01115803 Terminated Drug: LY2584702|Drug: Erlotinib|Drug: Everolimus Metastases Neoplasm|Carcinoma Non-small Cell Lung|Renal Cell Carcinoma|Neuroendocrine Tumors Eli Lilly and Company March 2010 Phase 1
NCT01394003 Terminated Drug: LY2584702 Advanced Cancer Eli Lilly and Company November 2008 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    what is the difference between S7698 and S7704? Ie., what does the tosylate group do?

  • 回答:

    S7698 is the free base of the compound and S7704 is its tosylate salt. Chemically, salt form is more stable and has a better solubility than free base, however, it also has a higher molecular weight. Biologically, these two forms have the same activities.

S6 Kinase Signaling Pathway Map

相关S6 Kinase产品

Tags: 购买LY2584702 | LY2584702供应商 | 采购LY2584702 | LY2584702价格 | LY2584702生产 | 订购LY2584702 | LY2584702代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID