BI-D1870

For research use only. Not for use in humans.

目录号:S2843

BI-D1870 Chemical Structure

CAS No. 501437-28-1

BI-D1870是一种ATP-竞争性S6 ribosome抑制剂,在无细胞试验中作用于RSK1/2/3/K4IC50分别为31 nM/24 nM/18 nM/15 nM,作用于RSK比作用于MST2,GSK-3β,MARK3,CK1和Aurora B选择性高10到100倍。BI-D1870 显示除了抗癌的属性包括活性氧的生成、提高内质网应激反应和诱导自噬。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 2224.03 现货
RMB 1416.04 现货
RMB 2189.74 现货
RMB 5493.86 现货
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客户使用Selleck生产的BI-D1870发表文献36篇:

产品安全说明书

S6 Kinase抑制剂选择性比较

生物活性

产品描述 BI-D1870是一种ATP-竞争性S6 ribosome抑制剂,在无细胞试验中作用于RSK1/2/3/K4IC50分别为31 nM/24 nM/18 nM/15 nM,作用于RSK比作用于MST2,GSK-3β,MARK3,CK1和Aurora B选择性高10到100倍。BI-D1870 显示除了抗癌的属性包括活性氧的生成、提高内质网应激反应和诱导自噬。
靶点
RSK4 [1]
(Cell-free assay)
RSK3 [1]
(Cell-free assay)
RSK2 [1]
(Cell-free assay)
RSK1 [1]
(Cell-free assay)
15 nM 18 nM 24 nM 31 nM
体外研究

BI-D1870抑制RSK1,RSK2,RSK3和RSK4,IC50为10-30 nM,但在100倍更高浓度时,也不显著抑制10种其他AGC激酶成员和40多个其他蛋白激酶。BI-D1870可渗透细胞的,作用于人类胚胎肾293细胞和大鼠-2细胞,可抑制RSK介导佛波酯和EGF诱导的糖原合酶激酶-3β和LKB1的磷酸化。BI-D1870作用于六种其他AGC激酶,不影响激动剂触发的底物磷酸化。而且,BI-D1870不抑制佛波酯或EGF诱导的CREB磷酸化。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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H9 M2LEO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJGg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkC2NFE6KM7:TT6= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
KARPAS-45 M3v0Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXMbnZ7UW6qaXLpeIlwdiCxZjDoeY1idiCNQWLQRXMuPDViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNVY2OjVizszNMi=> NFfoRZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
NCI-H720 M1KyV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXCdJZbUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFczOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iMD6xO|E6PCEQvF2u MlXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
CTV-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJcohq[mm2aX;uJI9nKGi3bXHuJGNVXi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTCwMlQ4QDd{IN88UU4> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
DU-4475 NWL6N4RwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mne3TY5pcWKrdHnvckBw\iCqdX3hckBFXS12NEe1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjVyNEG3JO69VS5? NVvwZ3o6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
A172 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjZTY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTCwMlUyPzB3IN88UU4> MmTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
EW-18 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDVSYtpUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC54MkKxNUDPxE1w MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
KU812 Mm\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PIWmlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvPjJ4MkOg{txONg>? M4XTfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
KP-N-YS MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fBWWlvcGmkaYTpc44hd2ZiaIXtZY4hU1BvTj3ZV{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC54OEKyN{DPxE1w NF3ER4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
SBC-1 NHXvN4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV:wR5c2UW6qaXLpeIlwdiCxZjDoeY1idiCVQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC54OUWyOEDPxE1w NEPWSWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
ECC10 NU\mfoFNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\CZVFtUW6qaXLpeIlwdiCxZjDoeY1idiCHQ1OxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC55MEC5OEDPxE1w NXfYT|RtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
GAMG MkPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHQUmV[UW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjd2N{G3JO69VS5? NFfIbW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
NCI-H209 NYrOcFAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkC5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjd4OEKg{txONg>? MoLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
MC-IXC M1;Scmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTuR3J4UW6qaXLpeIlwdiCxZjDoeY1idiCPQz3JXGMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwN{m1PFkh|ryPLh?= NVXYZmxCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
HSC-3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfyS|hLUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC56MUe2PUDPxE1w MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
IST-SL1 NGXNbohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXMTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjh2NUe0JO69VS5? Mn:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
LU-139 MnLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXDZpdKdmirYnn0bY9vKG:oIHj1cYFvKEyXLUGzPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC56NEm1PEDPxE1w NWTFPGFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
ML-2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFq1OHJKdmirYnn0bY9vKG:oIHj1cYFvKE2OLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvQDV3N{Wg{txONg>? MmTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
CAL-62 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3T1ZWlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLU[yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjh6MUWyJO69VS5? MlX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
HL-60 M2XJU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nF[WlvcGmkaYTpc44hd2ZiaIXtZY4hUExvNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvQDh|NkKg{txONg>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
KY821 MoPzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrsWoY3UW6qaXLpeIlwdiCxZjDoeY1idiCNWUiyNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC56OEixNkDPxE1w Ml60QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
ES7 M1flZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzjNJVGUW6qaXLpeIlwdiCxZjDoeY1idiCHU{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvQTF4MEeg{txONg>? M1TmPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
MV-4-11 NFy2SGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXR[WtKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwOUe4PVch|ryPLh?= NFexbYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
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EoL-1-cell MoW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG[3RZFKdmirYnn0bY9vKG:oIHj1cYFvKEWxTD2xMYNmdGxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEGuNFE5PzhizszNMi=> MonvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
TGW MlKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jVTWlvcGmkaYTpc44hd2ZiaIXtZY4hXEeZIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTCxMlA2OTJ2IN88UU4> M2HqNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
LAMA-84 NGfPO49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjETpR3UW6qaXLpeIlwdiCxZjDoeY1idiCOQV3BMVg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSBzLkGyO|k1KM7:TT6= MknsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
KOSC-2 M2n0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjzcIV7UW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDFwMUOzPFUh|ryPLh?= NF\oS3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
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CHP-134 NEDneIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJcohq[mm2aX;uJI9nKGi3bXHuJGNJWC1zM{SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFMvPDh5NUig{txONg>? NWLHZZc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
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NCI-H226 M330Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXJT3JCUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIzPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iM{[2MlY5OyEQvF2u NXfPXHdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd|MUC3M{c,W0GQR1XSQE9iRg>?
UACC-893 NGLkdIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPoeZA1UW6qaXLpeIlwdiCxZjDoeY1idiCXQVPDMVg6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iM{[4Mlk1QCEQvF2u Mnj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
OAW-28 MkPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\VZmhlUW6qaXLpeIlwdiCxZjDoeY1idiCRQWetNlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDN6Mz60PVMh|ryPLh?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+
NMC-G1 Ml\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3RTY5pcWKrdHnvckBw\iCqdX3hckBPVUNvR{GgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFM6PS57NEeg{txONg>? NF:0PVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
NCI-H1563 NX\T[4c2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUW2N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOzl6LkC5OkDPxE1w MkjWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
SW900 M3nTNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTByIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTC0NFEvOzJ4IN88UU4> M3zQUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
SW948 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rZW2lvcGmkaYTpc44hd2ZiaIXtZY4hW1d7NEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQyOS5yOUSg{txONg>? MoqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
CAPAN-1 M{LjOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJGNCWEGQLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQyOS5{OUKg{txONg>? M4rxSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
UACC-257 MmrXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;IZ3pKdmirYnn0bY9vKG:oIHj1cYFvKFWDQ1OtNlU4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSB2M{OuNVU2KM7:TT6= NG[zd2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
ZR-75-30 NUXheHozT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jpN2lvcGmkaYTpc44hd2ZiaIXtZY4hYlJvN{WtN|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDR2OT65N|Qh|ryPLh?= MnTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc{OTB5Lze+V2FPT0WUPD;hQi=>
NCI-H1993 M1zwPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDDT2ZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iNEiwMlI3PCEQvF2u NF;Gb|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
LB996-RCC NFHMfJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{j6bmlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ7OU[tVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSB2OUWuNlQyKM7:TT6= M3:4U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4OzFyNz:nQnNCVkeHUkyvZV4>
L-428 M3PxWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJcohq[mm2aX;uJI9nKGi3bXHuJGwuPDJ6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTC1OVQvPzhizszNMi=> NGHBfHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2PzNzMEevK|5USU6JRWK8M4E,
C3A M4ewTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkK2TY5pcWKrdHnvckBw\iCqdX3hckBEO0FiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEW2N{44QCEQvF2u MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1O|MyODdxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3 / p-ERK / ERK / p-CREB / CREB / p-S6 / p-LKB1 / LKB1 ; 

PubMed: 17040210     


(A) HEK-293 cells that had been deprived of serum for 16 h, were incubated in the absence or presence of 10 μM BI-D1870 for the indicated times, then stimulated with 400 ng/ml PMA (TPA) for 20 min. The cells were lysed and the lysates were immunoblotted with the indicated antibodies as described in the Materials and methods section. (B) As in (A), except that cells were incubated for 30 min with the indicated concentrations of BI-D1870 prior to stimulation with PMA. Similar results were obtained in two separate experiments.

p-YB1 / YB1 / p-Bad / Bad / p-RSK / RSK ; 

PubMed: 28415756     


Western Blot analysis of RSK activity (PS102-YB-1, PS112-Bad) of double resistant SKMel28 RR after treatment with increasing concentrations of BI-D1870 for 24 h. GAPDH was detected as a loading control.

IRF4 / MYC / MCL-1 / PARP ; 

PubMed: 25395420     


(B) H929 cells were incubated with BI-D1870 (BI, 3 μM) at indicated time points, followed by immunoblotting analysis. (C) JJN3 cells were incubated with BI-D1870 at indicated doses for 6 hours, followed by immunoblotting analysis.

p-GSK3 / p-ERK / ERK / p-CREB / CREB / p-S6 / p-LKB1 / LKB1; 

PubMed: 17040210     


(A) HEK-293 cells that had been deprived of serum for 16 h, were incubated in the absence or presence of 10 μM BI-D1870 for the indicated times, then stimulated with 400 ng/ml PMA (TPA) for 20 min. The cells were lysed and the lysates were immunoblotted with the indicated antibodies as described in the Materials and methods section. (B) As in (A), except that cells were incubated for 30 min with the indicated concentrations of BI-D1870 prior to stimulation with PMA. Similar results were obtained in two separate experiments.

p-YB1 / YB1 / p-Bad / Bad / p-RSK / RSK ; 

PubMed: 28415756     


Western Blot analysis of RSK activity (P(S102)-YB-1, P(S112)-Bad) of double resistant SKMel28 RR after treatment with increasing concentrations of BI-D1870 for 24 h. GAPDH was detected as a loading control.

IRF4 / MYC / MCL-1 / PARP ; 

PubMed: 25395420     


(B) H929 cells were incubated with BI-D1870 (BI, 3 μM) at indicated time points, followed by immunoblotting analysis. (C) JJN3 cells were incubated with BI-D1870 at indicated doses for 6 hours, followed by immunoblotting analysis.

17040210 28415756 25395420
Immunofluorescence
PDCD4 / F-actin ; 

PubMed: 25002506     


A375 cells treated as in E were imaged using immunofluorescence microscopy. Cells were stained with anti-PDCD4 antibodies to visualize endogenous PDCD4, phalloidin to visualize F-actin, and DAPI to visualize nuclei.

PDCD4 / F-actin ; 

PubMed: 25002506     


A375 cells treated as in (E) were imaged using immunofluorescence microscopy. Cells were stained with anti-PDCD4 antibodies to visualize endogenous PDCD4, phalloidin to visualize F-actin, and DAPI to visualize nuclei.

25002506
Growth inhibition assay
Cell viability; 

PubMed: 28415756     


(D–F) Cell viability (MUH assay) of double resistant melanoma cells after a 72 h-treatment with increasing concentrations of vemurafenib (D), trametinib (E) or vemurafenib and trametinib (F), as well as of BI-D1870 and the combination of MAPK inhibitors and BI-D1870 (n = 6; mean ± SD). Signals were normalized to the DMSO-treated controls.

Cell viability; 

PubMed: 28415756     


(D–F) Cell viability (MUH assay) of double resistant melanoma cells after a 72 h-treatment with increasing concentrations of vemurafenib (D), trametinib (E) or vemurafenib and trametinib (F), as well as of BI-D1870 and the combination of MAPK inhibitors and BI-D1870 (n = 6; mean ± SD). Signals were normalized to the DMSO-treated controls.

28415756

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 78 mg/mL (199.27 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 391.42
化学式

C19H23F2N5O2

CAS号 501437-28-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    BI-D1870 (catalog No.S2843) is found as a racemic mixture?

  • 回答:

    Yes, BI-D1870 (catalog No.S2843) is found as a racemic mixture.

S6 Kinase Signaling Pathway Map

相关S6 Kinase产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID