AZD7762

For research use only. Not for use in humans.

目录号:S1532

AZD7762 Chemical Structure

CAS No. 860352-01-8

AZD7762是一种有效的,选择性Chk1抑制剂,无细胞试验中IC50为5 nM,作用于Chk2也同样有效,对CAM, Yes, Fyn, Lyn, Hck和Lck作用效果稍弱。Phase 1。

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RMB 576.88 现货
RMB 974.33 现货
RMB 3040.33 现货
RMB 7938.28 现货
RMB 13243.23 现货
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客户使用Selleck生产的AZD7762发表文献80篇:

产品安全说明书

Chk抑制剂选择性比较

生物活性

产品描述 AZD7762是一种有效的,选择性Chk1抑制剂,无细胞试验中IC50为5 nM,作用于Chk2也同样有效,对CAM, Yes, Fyn, Lyn, Hck和Lck作用效果稍弱。Phase 1。
靶点
Chk1 [1]
(Cell-free assay)
Chk2 [1]
(Cell-free assay)
5 nM <10 nM
体外研究

AZD7762是Chk1选择性抑制剂, 通过与Chk1的ATP结合位点可逆结合而抑制cdc25C肽段的Chk1磷酸化,IC50 为5 nM,Ki为 3.6 nM。AZD7762诱导细胞周期停滞,EC50为0.620 μM, 且通过阻断cdc25A依赖chk1的降解 和Cyclin A的激活,显著废除Camptothecin诱导的G2期停滞,EC50为10 nM。300 nM AZD7762增强Gemcitabine作用于SW620的抗癌活性,也增强 Topotecan作用于MDA-MB-231 的抗癌活性,通过降低 GI50 值,分别从24.1 nM和2.25 μM 降到1.08 nM 和0.15 μM。[1] AZD7762 作用于多种携带p53野生型,p53突变型,MDM2增添,或p14 缺失的神经母细胞瘤细胞系,IC50为82.6 nM 到505.9 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KASUMI-1 M{jqfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnOSlhKSzVyPUCuNFAxOjN2IN88US=> MnOzV2FPT0WU
NKM-1 MoDOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHBb3oyUUN3ME2wMlAxODRzNTFOwG0> MUfTRW5ITVJ?
MV-4-11 NW\JZ4ZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTBwMECwOFI2KM7:TR?= M2i0Z3NCVkeHUh?=
NCI-H1703 M370fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPXWGZHUUN3ME2wMlAxPjR|IN88US=> M1LYXXNCVkeHUh?=
A204 NYrQV5I2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELrfphKSzVyPUCuNFA5PTZizszN Ml7sV2FPT0WU
CGTH-W-1 NX6xN4o1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HMWWlEPTB;MD6wNVQzKM7:TR?= MniyV2FPT0WU
MLMA NIrJc5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkK0TWM2OD1yLkCyPFQ6KM7:TR?= NELaOJhUSU6JRWK=
GOTO NHLoelZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnDTWM2OD1yLkCzNVIh|ryP MnrGV2FPT0WU
KE-37 NYO3[FJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nFVmlEPTB;MD6wN|E1OiEQvF2= NV3TZYlKW0GQR1XS
ES4 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMEOyOVEh|ryP M3fYPXNCVkeHUh?=
ATN-1 M1zYUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTBwMEO0NVch|ryP MVzTRW5ITVJ?
MHH-PREB-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTBwMEO0OFIh|ryP MoTzV2FPT0WU
SW756 MkT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTBwMEO1O|kh|ryP NVu2NYozW0GQR1XS
CTV-1 NGPqfW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MluxTWM2OD1yLkCzPFk3KM7:TR?= MUfTRW5ITVJ?
HCC1806 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXqTWM2OD1yLkC0NFIzKM7:TR?= NEe0XWZUSU6JRWK=
NEC8 NVLqVGx4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1v5bmlEPTB;MD6wOFIxOyEQvF2= M3frVXNCVkeHUh?=
P12-ICHIKAWA MmTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDhfVhKSzVyPUCuNFQ{OTZizszN M3LDfXNCVkeHUh?=
DEL Ml22S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTQTWM2OD1yLkC0OVkzKM7:TR?= NUTm[oFnW0GQR1XS
KM12 NF:wSWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jzNGlEPTB;MD6wOVE2QCEQvF2= NWT2ZXRbW0GQR1XS
LB1047-RCC NFfSN4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETjbHNKSzVyPUCuNFYxOjVizszN M1[4RXNCVkeHUh?=
LB2241-RCC MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHoT3JKSzVyPUCuNFYyODJizszN M4DqNXNCVkeHUh?=
NOMO-1 NUD3NWt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTBwME[xNVUh|ryP MoDZV2FPT0WU
NB14 M37rSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrtTWM2OD1yLkC2NVM3KM7:TR?= NYDTfIV4W0GQR1XS
ONS-76 NIfQSHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LBcGlEPTB;MD6wOlE6PiEQvF2= M373RXNCVkeHUh?=
HD-MY-Z NFqySlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTBwME[yOVUh|ryP MXPTRW5ITVJ?
G-402 Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;XWmtKSzVyPUCuNFY1PiEQvF2= MnzLV2FPT0WU
ES1 Mn3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\uXI43UUN3ME2wMlA4OTR4IN88US=> M1rMTnNCVkeHUh?=
L-363 NIXYbXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrQTpBKSzVyPUCuNFc{QSEQvF2= Mn;CV2FPT0WU
A4-Fuk NXS4e2ptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfVTWM2OD1yLkC3OFg3KM7:TR?= M{mxU3NCVkeHUh?=
OC-314 NELmSHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjZdHI6UUN3ME2wMlA4QDB5IN88US=> MlvlV2FPT0WU
SK-LU-1 NGjkPY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\jR3RKSzVyPUCuNFg1OjFizszN NGjTeoxUSU6JRWK=
MDA-MB-157 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;tSmlEPTB;MD6wPFY2QSEQvF2= NIrafIdUSU6JRWK=
HuP-T3 M1;LWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPjW25zUUN3ME2wMlA5PzFizszN NYf6N3RPW0GQR1XS
A3-KAW NH7NR4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfYTldKSzVyPUCuNFg5ODZizszN NGXqUnFUSU6JRWK=
MOLT-16 NU\ZOoxxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjhOZFKUUN3ME2wMlA5QDB5IN88US=> M{f5PHNCVkeHUh?=
COLO-679 Ml76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwMEmwPVEh|ryP NX;3epFqW0GQR1XS
EM-2 M3XxeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Pne2lEPTB;MD6wPVkxOyEQvF2= MmTiV2FPT0WU
ES8 Mny5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{OycGlEPTB;MD6wPVkyQCEQvF2= MXPTRW5ITVJ?
SK-MEL-2 NFTkNJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTBwMUCwN|Mh|ryP MoPyV2FPT0WU
ES3 NFHXNmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInvWmNKSzVyPUCuNVAzKM7:TR?= NW\XUYRMW0GQR1XS
HSC-4 NIPR[lNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTBwMUC1OVQh|ryP NFL0SJVUSU6JRWK=
697 MlrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzVcHdKSzVyPUCuNVExPjNizszN NXfaV4E4W0GQR1XS
TGBC11TKB MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4n5WWlEPTB;MD6xNVA6OyEQvF2= MXPTRW5ITVJ?
SK-PN-DW NYLENYpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\4XWlEPTB;MD6xNVg3KM7:TR?= M1rOOXNCVkeHUh?=
H9 NH7NNZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMUKxOlUh|ryP MW\TRW5ITVJ?
NCI-H2122 NITwZ4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHsd4FoUUN3ME2wMlEzOjVizszN M3jEdHNCVkeHUh?=
ML-2 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlroTWM2OD1yLkGyOFg1KM7:TR?= M2m2PXNCVkeHUh?=
MC-IXC MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIi4V29KSzVyPUCuNVI5PDNizszN NGm4OI5USU6JRWK=
KYSE-140 M3ixW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILxPJlKSzVyPUCuNVMxPDFizszN NHvuNpBUSU6JRWK=
DOHH-2 NFfqXoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13CfGlEPTB;MD6xN|A3PCEQvF2= NHy4OpNUSU6JRWK=
MEL-JUSO NFXBRWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwMUOxNlgh|ryP Ml3sV2FPT0WU
P30-OHK NYTISYs{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwMUOxOkDPxE1? M3\VfnNCVkeHUh?=
CTB-1 NXzSVHFbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDFTWM2OD1yLkGzN|I2KM7:TR?= MoPIV2FPT0WU
ACHN NYOwZ4RYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTBwMUOzOlkh|ryP MmPnV2FPT0WU
ES5 NWDQV|l5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnETWM2OD1yLkGzOFc3KM7:TR?= MXzTRW5ITVJ?
SF539 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTZfXdYUUN3ME2wMlE{PjhizszN NWLVbYlQW0GQR1XS
D-283MED NVm5blRiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEn1SZdKSzVyPUCuNVM3QDdizszN NUPENY1iW0GQR1XS
OVCAR-5 MlK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFq0XVNKSzVyPUCuNVM5QTRizszN MVXTRW5ITVJ?
RPMI-8866 MoLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLVZYNKSzVyPUCuNVM6OjVizszN MYHTRW5ITVJ?
PANC-03-27 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTBwMUSyPFgh|ryP NETVSY9USU6JRWK=
M14 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVWwcFRVUUN3ME2wMlE1PDZ|IN88US=> MWHTRW5ITVJ?
D-566MG MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPGTWM2OD1yLkG0Olkh|ryP MnzKV2FPT0WU
Ramos-2G6-4C10 NYTjWFkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYX4[VR6UUN3ME2wMlE1QDR{IN88US=> Mo\iV2FPT0WU
NOS-1 NGPvU4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfVTWM2OD1yLkG0PVY4KM7:TR?= NXfrTW5nW0GQR1XS
YH-13 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4juc2lEPTB;MD6xOVY4PCEQvF2= NXvSWHJmW0GQR1XS
NB17 M1L6NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDWNFFKSzVyPUCuNVU4QDZizszN MnPoV2FPT0WU
U-2-OS M1SwOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTBwMUW4OFch|ryP NXL0cIZtW0GQR1XS
ES7 NVvYTHZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3YPG1KSzVyPUCuNVYxOTFizszN MlX3V2FPT0WU
LoVo NVj5ZWdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fGe2lEPTB;MD6xOlE3PiEQvF2= MnXlV2FPT0WU
QIMR-WIL Mn7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXW1R2dqUUN3ME2wMlE3OzFzIN88US=> NYe5TJdLW0GQR1XS
ESS-1 M3LTOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPsW2lmUUN3ME2wMlE3PDF|IN88US=> MU\TRW5ITVJ?
LAMA-84 NUfnfFFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwMU[1NFMh|ryP NH\DTJZUSU6JRWK=
TGBC24TKB NVzQO4x7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfOdY9uUUN3ME2wMlE3PTN3IN88US=> M3TYOXNCVkeHUh?=
KU812 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPWTWM2OD1yLkG2PFMh|ryP NYDsXVQxW0GQR1XS
KARPAS-45 MoD6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDoTWM2OD1yLkG2PFQ4KM7:TR?= MoDqV2FPT0WU
ACN MlvIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwMUexO|Eh|ryP NHPlSGNUSU6JRWK=
RPMI-8226 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwMUe0OlQh|ryP NFj6TFRUSU6JRWK=
GR-ST NETEZYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwMUe4Nlch|ryP MWLTRW5ITVJ?
SK-UT-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjPO3JKSzVyPUCuNVg2QTlizszN NX7XNJVPW0GQR1XS
SK-N-AS M3LXOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYn4V5RpUUN3ME2wMlE5QDF3IN88US=> MlLlV2FPT0WU
EW-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETzcoRKSzVyPUCuNVg6ODVizszN NUf0NmN[W0GQR1XS
5637 M3;kUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnhU5BCUUN3ME2wMlE6Ojd6IN88US=> MWfTRW5ITVJ?
GI-1 Mn\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjjTm5uUUN3ME2wMlE6PTZzIN88US=> NHTjVFNUSU6JRWK=
NCI-H1395 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfTcGlLUUN3ME2wMlE6PzZzIN88US=> M3r4dXNCVkeHUh?=
CHP-212 NVrCVIFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwMUm3PFIh|ryP MoTRV2FPT0WU
A427 M1;mVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorhTWM2OD1yLkG5PFMzKM7:TR?= M1;Z[nNCVkeHUh?=
BE-13 M{\IT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvkSVBKSzVyPUCuNlAyOTdizszN MUTTRW5ITVJ?
RS4-11 NXT3SW1[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIf5eZVKSzVyPUCuNlA{OThizszN NFjXPFdUSU6JRWK=
TI-73 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwMkC4O|Mh|ryP NFvMdVdUSU6JRWK=
HAL-01 Ml3ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HUZWlEPTB;MD6yNFk4KM7:TR?= Ml;jV2FPT0WU
KY821 M13TbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELOO2hKSzVyPUCuNlE1OjlizszN MVLTRW5ITVJ?
H4 NWTpPWRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\hTWM2OD1yLkKyNFYzKM7:TR?= NXvQT2kzW0GQR1XS
EPLC-272H MkXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\3TWM2OD1yLkKyNFY5KM7:TR?= M3rCPHNCVkeHUh?=
COR-L105 NYfLXZlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jLTWlEPTB;MD6yNlI{PSEQvF2= M2n4dnNCVkeHUh?=
769-P MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1y1ZmlEPTB;MD6yNlI6QCEQvF2= MXfTRW5ITVJ?
HEL NH\vfnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1m0TmlEPTB;MD6yNlQ2PCEQvF2= MWTTRW5ITVJ?
VA-ES-BJ NGO5fnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjhTWM2OD1yLkKyOVU4KM7:TR?= MWXTRW5ITVJ?
IST-MEL1 M{P1Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmX5TWM2OD1yLkKyOVch|ryP Mlv3V2FPT0WU
KYSE-270 NVrwUGNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnUd5R5UUN3ME2wMlIzPjN|IN88US=> NX;hVpM4W0GQR1XS
RH-1 M{DFO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4H0NGlEPTB;MD6yNlY3PiEQvF2= MoDjV2FPT0WU
JVM-3 NFP1Rm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTBwMkOwN|Yh|ryP M2LLdnNCVkeHUh?=
RPMI-7951 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTBwMkOwOVYh|ryP MmPRV2FPT0WU
JAR NVnYWINXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE[1[4NKSzVyPUCuNlMzOzhizszN MXPTRW5ITVJ?
MOLT-4 MoL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonlTWM2OD1yLkKzPFUh|ryP NWThNm1PW0GQR1XS
ALL-PO Ml71S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmH1TWM2OD1yLkK0NlQ{KM7:TR?= MnK2V2FPT0WU
SNU-387 MlLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnqzTWM2OD1yLkK0N|M6KM7:TR?= MmfGV2FPT0WU
IGROV-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn[1TWM2OD1yLkK0OFAyKM7:TR?= M1zIdHNCVkeHUh?=
MPP-89 NITaRmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULyV245UUN3ME2wMlI1PDVzIN88US=> NVHEfI5DW0GQR1XS
T98G MoHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkC3TWM2OD1yLkK0OVY5KM7:TR?= M1vUOXNCVkeHUh?=
NCI-H510A M{\wcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTBwMkS2NlYh|ryP Ml3wV2FPT0WU
KOSC-2 M3z0UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMkS2O|Eh|ryP M4C2dnNCVkeHUh?=
NCI-H23 NEL4NG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW[xWmFUUUN3ME2wMlI1PzZzIN88US=> Mo\SV2FPT0WU
MC116 NWLrcI9TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1SzPWlEPTB;MD6yOVY2PCEQvF2= MUnTRW5ITVJ?
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SW1710 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTBwNkSwNlMh|ryP NIfUfmVUSU6JRWK=
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LU-134-A MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwNkW2OUDPxE1? M4nE[XNCVkeHUh?=
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23132-87 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;CeXlKSzVyPUCuOlg{ODZizszN MVPTRW5ITVJ?
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A172 M4j3[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXe5ZnBVUUN3ME2wMlY6OjF5IN88US=> NVXwd5lWW0GQR1XS
NCI-H727 Mn3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofYTWM2OD1yLk[5PFEzKM7:TR?= MnztV2FPT0WU
NB69 Mn:4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEm0eXpKSzVyPUCuO|A2PDlizszN NGDNOFhUSU6JRWK=
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HuCCT1 M3vXVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUO1ZolmUUN3ME2wMlcxPzZ3IN88US=> NUXTWFJZW0GQR1XS
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SW1417 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHyXFRSUUN3ME2wMlc{PTl|IN88US=> NXf4SZB[W0GQR1XS
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CAL-54 NFvjVItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwN{SwN{DPxE1? MWTTRW5ITVJ?
Saos-2 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3aPYdZUUN3ME2wMlc1PTh2IN88US=> M1;kT3NCVkeHUh?=
NCI-H446 M3TGVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\ZSWlEPTB;MD63OFgxPiEQvF2= M{nyOXNCVkeHUh?=
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PA-1 M4LxbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPyXlZPUUN3ME2wMlc1QTZ6IN88US=> NYK2Upl2W0GQR1XS
HCC38 M3ftUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrz[3FnUUN3ME2wMlc1QTh2IN88US=> MYDTRW5ITVJ?
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HDLM-2 M1fCSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTBwOEGzOFkh|ryP NW\CUHh{W0GQR1XS
HEC-1 M{PtTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2X0ZmlEPTB;MD64NVM4OSEQvF2= MVfTRW5ITVJ?
AU565 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwOEK0OEDPxE1? Mn3DV2FPT0WU
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LN-405 M3f3SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTBwOEe3N|Eh|ryP M1rWV3NCVkeHUh?=
HOS NEXIdnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUH1clF4UUN3ME2wMlg4QTl3IN88US=> MXrTRW5ITVJ?
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NCI-H292 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDxSlY2UUN3ME2wMlg6Pzd5IN88US=> M4DJSnNCVkeHUh?=
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TE-10 M3m1WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHGTWM2OD1yLkmwPVg1KM7:TR?= M{DId3NCVkeHUh?=
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NCI-H1048 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\hZWlEPTB;MD65NVg2OiEQvF2= MYfTRW5ITVJ?
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NCI-H1792 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfGNoFjUUN3ME2wMlk1OjN5IN88US=> NHTQVI9USU6JRWK=
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KYSE-410 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTFwMEO1NkDPxE1? NGKzcYZUSU6JRWK=
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NCI-H1651 MmrPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXRTWM2OD1zLkC5N|UzKM7:TR?= NXj2fWxoW0GQR1XS
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TE-8 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTDfVJpUUN3ME2xMlEzOjlzIN88US=> NH7KWY1USU6JRWK=
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SNB75 Mn3PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTFwMUm4NVkh|ryP NH\Wb5FUSU6JRWK=
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CAS-1 MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fV[GlEPTB;MT6yNVQyQCEQvF2= NUnadWlnW0GQR1XS
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NCI-H1693 NV7nWI1CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLMb5U6UUN3ME2xMlIzPzV6IN88US=> NX3IcIlqW0GQR1XS
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NCI-H2347 M4\RO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTvTINkUUN3ME2xMlI{PTJ3IN88US=> NV\qd5BYW0GQR1XS
MFM-223 Mm\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jTcGlEPTB;MT6yOFM1OyEQvF2= NXzocmd4W0GQR1XS
OCI-AML2 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnu5TWM2OD1zLkK2OlY3KM7:TR?= MYrTRW5ITVJ?
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SK-MEL-30 NV;jUJdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzmRVhyUUN3ME20MlQyQTF|IN88US=> MnThV2FPT0WU
KURAMOCHI MnG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHweGRKSzVyPUSuOFU2OjhizszN MVHTRW5ITVJ?
BEN NFHnc4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTRwNUKzOlIh|ryP MmLQV2FPT0WU
NCI-H226 M33UXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHpVZZFUUN3ME20MlU1QDdzIN88US=> M4HqbHNCVkeHUh?=
CAL-27 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjoUFVxUUN3ME20MlcyPjl2IN88US=> NFXMOohUSU6JRWK=
G-401 Mn\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfOTWM2OD12LkizPFQyKM7:TR?= NF3RSHpUSU6JRWK=
CAL-120 NYL2SXpvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnvUGlLUUN3ME20Mlg1QTlzIN88US=> M33z[XNCVkeHUh?=
DMS-114 Mk\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTVwMUS1NFYh|ryP MkLXV2FPT0WU
KYSE-70 NUHyd2RET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\hTWM2OD13LkG0PFU6KM7:TR?= NVqzVIdGW0GQR1XS
NCI-H1648 NF[5cFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfBTWM2OD13LkK5O|gzKM7:TR?= NFrxSIhUSU6JRWK=
MDA-MB-175-VII Mo\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPCTWM2OD13LkS0NFE1KM7:TR?= NH6yO|JUSU6JRWK=
NCI-H2029 NHfq[HdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnCbIRKSzVyPUWuOFgxOjJizszN NFv1R3VUSU6JRWK=
LS-1034 NH\DcG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnXOmxKSzVyPUWuOlY5QDlizszN MUfTRW5ITVJ?
LB831-BLC M1\rNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\OOGlEPTB;NT63NFM1PSEQvF2= M4jHWXNCVkeHUh?=
MDA-MB-415 NGP6VXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\JTWM2OD13LkewPFEh|ryP M4TTcnNCVkeHUh?=
TE-6 NX\1R|d2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\KTWM2OD13Lke0NVgzKM7:TR?= NY[xdGViW0GQR1XS
SK-MEL-24 NEnTTFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2H0b2lEPTB;NT63OFU3PCEQvF2= NHPKToFUSU6JRWK=
HUTU-80 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrBOo16UUN3ME21Mlc2OzN|IN88US=> MofDV2FPT0WU
OCUB-M NUfTe5BUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDPc5JzUUN3ME21Mlc4PTZ|IN88US=> NVvKZnR3W0GQR1XS
IGR-1 M1LCeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnUb5VKSzVyPUWuPFY5QTRizszN MmXDV2FPT0WU
NCI-H747 NVrofFZWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LX[2lEPTB;Nj6yOFU5PSEQvF2= MmTMV2FPT0WU
COLO-680N M1HoeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTZwM{m0NFMh|ryP M4TWO3NCVkeHUh?=
NB12 Ml36S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTZwN{m3NlIh|ryP M17OdnNCVkeHUh?=
SNU-449 M2nobGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPrcWFIUUN3ME22Mlk6PTlzIN88US=> Ml2yV2FPT0WU
D-542MG MmnYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTdwMUKyPVkh|ryP NHTubWRUSU6JRWK=
COLO-792 Mlv2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PwSmlEPTB;Nz64O|EzPyEQvF2= NGXSS5BUSU6JRWK=
NCI-H810 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoO5TWM2OD16LkCyPVg1KM7:TR?= M{LH[nNCVkeHUh?=
T47D NFLYSJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEO2PIdKSzVyPUiuNVYxOjRizszN NHLpVm9USU6JRWK=
Calu-3 NILBOo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRThwMki4PFYh|ryP NGr0S3RUSU6JRWK=
COLO-741 NVvoeHBqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVqxWoNJUUN3ME24Mlk{ODl|IN88US=> MlvFV2FPT0WU
LU-135 NV;ZXVFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTlwMEO0OVIh|ryP NWXGdnpiW0GQR1XS
COLO-678 M3jKUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfJ[Y8{UUN3ME25MlI5QDR2IN88US=> NGXvbWhUSU6JRWK=
NCI-H2126 M3jNemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTlwM{O5NVkh|ryP NFj0PWJUSU6JRWK=
SK-MEL-28 NVfKXZNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnJTWM2OD17LkWzOVk3KM7:TR?= MmLXV2FPT0WU
MFE-280 NIS2WYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrPXZlKSzVyPUGwMlAzOjlizszN M3O4VnNCVkeHUh?=
DBTRG-05MG NX\sOnBxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETzR25KSzVyPUGwMlQ{PjlizszN M1TscHNCVkeHUh?=
RCC10RGB NYXjSIE6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DWUmlEPTB;MUCuOVI2PSEQvF2= MmPCV2FPT0WU
NTERA-S-cl-D1 NE[4XG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1[xdWlEPTB;MUCuOlg2OiEQvF2= NWPBVYVqW0GQR1XS
C3A NI[zSpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfW[XFKSzVyPUGyMlMyPzJizszN NHfmUZpUSU6JRWK=
MHH-ES-1 NFS3WnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTF{LkWyPFQh|ryP MV\TRW5ITVJ?
LU-139 NGnyd|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUf1PVJ3UUN3ME2xNk43OzN|IN88US=> MX\TRW5ITVJ?
KNS-42 NFTIWmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTyOWoyUUN3ME2xNk45PzdzIN88US=> Ml;EV2FPT0WU
MCF7 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2e3d2lEPTB;MUOuNlM3PSEQvF2= MoLLV2FPT0WU
SW872 MojMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHGO5FKSzVyPUG0MlU1PjFizszN NXXDZnBDW0GQR1XS
HT-1376 M4S4TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHj[FRiUUN3ME2xOE45PTh{IN88US=> NEXjRo9USU6JRWK=
NCI-SNU-5 NXi0WGJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTF3LkG3OFEh|ryP MWLTRW5ITVJ?
NCI-H2405 NET4NHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\0bYM{UUN3ME2xOU41PDd2IN88US=> MX3TRW5ITVJ?
KYSE-150 NX7NUlRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;Ye|NKSzVyPUG1Mlc3OTFizszN NELMNpVUSU6JRWK=
SW837 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXS5WZhIUUN3ME2xOk4xOTJ2IN88US=> MnTFV2FPT0WU
LU-65 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTF7LkeyOkDPxE1? NUnGbJpYW0GQR1XS
JEG-3 MnjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTJyLkO1O|ch|ryP NIKyVYRUSU6JRWK=
SK-MEL-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUP0fmxRUUN3ME2yNE44QDN4IN88US=> MkS5V2FPT0WU
SK-HEP-1 NUDD[IRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTJzLkW1OFEh|ryP MmHpV2FPT0WU
NCI-H1770 NVXGUYxrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mof6TWM2OD1{Mz60NFg3KM7:TR?= NVTReYh3W0GQR1XS
MG-63 NEGyfGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmj3TWM2OD1{Mz63Olc{KM7:TR?= M1v3fXNCVkeHUh?=
ECC10 NYDSeGhTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DwOWlEPTB;MkWuOVcyKM7:TR?= MYnTRW5ITVJ?
SW1463 M2rrTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXETWM2OD1{Nz64N|E3KM7:TR?= Ml\PV2FPT0WU
JVM-2 M3i5Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnGwTWM2OD1{OD6zNlQ5KM7:TR?= M3;Xc3NCVkeHUh?=
HCT-15 NGnIcXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vBU2lEPTB;MkiuOFE6OiEQvF2= M{PVfnNCVkeHUh?=
CAL-72 MkDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDWTWM2OD1|MT60PVEzKM7:TR?= Mlq0V2FPT0WU
HCC2218 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1G2WmlEPTB;M{KuPFg2QCEQvF2= MVLTRW5ITVJ?
BT-474 NV3CeFg4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkW1TWM2OD1|Mz6wPFQ{KM7:TR?= MWXTRW5ITVJ?
NCI-H1092 MnLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\I[nQ2UUN3ME2zN{42OTR3IN88US=> MXnTRW5ITVJ?
Mo-T M3nzdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rrWGlEPTB;M{euN|U5PSEQvF2= NGnLT5dUSU6JRWK=
DU-145 MoX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\EcJFKSzVyPUSwMlYzQTVizszN NVLSepVMW0GQR1XS
NCI-H1666 NXfMS4tVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvFbmVKSzVyPUSyMlQ1QDhizszN NYT1WI1SW0GQR1XS
IST-MES1 NUOzZoxkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTR4LkSxPVYh|ryP MXzTRW5ITVJ?
SW1116 MorDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4Tub2lEPTB;NEiuNVc2PiEQvF2= NX3sUJUyW0GQR1XS
MZ2-MEL MmrVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jHRmlEPTB;NEiuOVAzPiEQvF2= NEjMSo1USU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pChk1 / Chk1 / Wee1 / p21 / p53 ; 

PubMed: 29963769     


Immunoblotting analysis of CAL39 and SW954 cells showing increase in CHK1 phosphorylation after exposure to AZD7762 for 24 hours. β‐actin is used as loading control. Representative data from n = 3 experiments.

29963769
Growth inhibition assay
Cell viability; 

PubMed: 23715154     


Lkb1-wt (634, 855, and 857) and Lkb1-null (t2, t4, and t5) cell lines were plated into 96-well plates at 2000 cells/well in 150 ml medium containing the indicated concentrations of AZD7762 or CHIR124 for 3 days. Viable cells were then counted with Dojindo’s Cell Counting Kit-8 assay. The data represent mean ± SD for 3 repeats. GI50 was calculated with GraphPad.

23715154
体内研究 AZD7762 按25 mg/kg剂量单独作用于携带H460-DNp53移植瘤的小鼠和携带SW620移植瘤的小鼠,显示很弱的抗癌活性,但是和Gemcitabine(60 mg/kg)联用处理这两种携带移植瘤的小鼠,则具有强抗癌活性。AZD7762和Gemcitabine(10 mg/kg) 联用作用于携带H460-DNp53移植瘤大鼠,抑制肿瘤体积,这种作用存在剂量依赖性。AZD7762 (25 mg/kg)和Irinotecan (25或50 mg/kg) 联用作用于携带SW620移植瘤的小鼠,引起全部肿瘤衰退。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

Chk1激酶实验:

使用杆状病毒载体,融合在昆虫细胞中,重组人类Chk1表达作为谷胱甘肽S转移酶,通过胱甘肽亲和层析纯化。合成的肽底物 (N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR)用于Chk1 合成。实验中,肽和ATP(完全和40 nCi [33P]ATP) 的终浓度分别为0.8和1 μM。不同浓度AZD7762, 含肽和chk1激酶和ATP的buffer相继加到384孔板中。然后温育2小时,加入含EDTA和邻近闪烁分析(SPA)圆球微粒的buffer终止反应, 然后使用TopCount计数器读数。进行数据分析,测定IC50值。
细胞实验:[1]
- 合并
  • Cell lines: HT29,SW620和MDA-MB-231细胞
  • Concentrations: 溶于DMSO, 终浓度为12.5 μM 左右
  • Incubation Time: 20或48小时
  • Method: 检测点废除实验中, 用Camptothecin(拓扑异构酶I 抑制剂;0.07 μg/mL)处理HT29细胞2小时,诱导产生G2期检测点。然后用AZD7762(12.5 μM 到6 nM)12点滴定法和Nocodazole处理细胞20分钟。细胞和3.7%甲醛混合1小时,用含0.05% Triton X的PBS渗透处理, 然后与anti-phH3抗体温育1小时,然后用Hoechst染料染色1小时。使用ArrayScan测定有丝分裂指数,作为进行有丝分裂细胞的百分数。增强实验中,用Gemcitabine 9点滴定(0.01到 100 nM)和固定剂量AZD7762(300 nM)处理SW620或MDA-MB-231细胞24小时。24小时后, 移除培养基,单独加入AZD7762,再处理24小时。细胞在无AZD7762培养基中再温育72小时,通过MTT测定细胞增殖。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 皮下注射H460-DNp53细胞或SW620细胞的雄性NCr小鼠, 或皮下注射H460-DNp53细胞的雄性rnu大鼠
  • Dosages: ~25 mg/kg
  • Administration: 静脉注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 50 mg/mL (137.96 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 362.42
化学式

C17H19FN4O2S

CAS号 860352-01-8
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    If I want to use the inhibitor for i.p. injection, can I use DMSO to dissolve it?

  • 回答:

    Since DMSO has low toxicity with i.p. injection, hydroxyproplyl-β-cyclodextrin is consider a safer co-solvent in compound formulation. In a previous literature report, 11.3% hydroxyproplyl-β-cyclodextrin was used to formulate this compound for i.v. injection.

Chk Signaling Pathway Map

Chk Inhibitors with Unique Features

相关Chk产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID