MG132

目录号:S2619 别名: (S,R,S)-(-)-MG-132, Z-Leu-D-Leu-Leu-al

仅限科研使用

MG132 ((S,R,S)-(-)-MG-132, Z-Leu-D-Leu-Leu-al)是一种有效的蛋白酶体(ChTL, TL和PGPH)抑制剂。MG132对calpain也有抑制作用(IC50=1.2 μM)。

MG132 Chemical Structure

CAS: 1211877-36-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 819.69 现货
RMB 647.01 现货
RMB 1395.18 现货
RMB 3841.93 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

Selleck的MG-132蛋白酶体抑制效果显著高于Sigma同类产品,正是因为采用了效果最好的(S,R,S)-(-)-2构型

p21蛋白可以被泛素化降解,MG-132通过抑制蛋白酶体活性导致了p21的累积,因此,MG-132抑制效果越强,p21蛋白累积越多。(检测蛋白:p21; 细胞种类:HeLa细胞系; 处理条件:终浓度5μM,处理时间6h)

客户使用Selleck生产的MG132发表文献1658篇:

质量管理及产品安全说明书

Proteasome抑制剂选择性比较

相关Proteasome产品

生物活性

产品描述 MG132 ((S,R,S)-(-)-MG-132, Z-Leu-D-Leu-Leu-al)是一种有效的蛋白酶体(ChTL, TL和PGPH)抑制剂。MG132对calpain也有抑制作用(IC50=1.2 μM)。
靶点
Proteasome [9]
(Cell-free assay)
100 nM
体外研究

MG-132抑制20S 蛋白酶体ZLLL-MCA降解活性,IC50为100 nM,比ZLLal(IC50为110 μM)有效1000多倍。MG-132也会抑制钙蛋白酶,IC50 为1.2 μM。MG-132在20 nM的最佳浓度下诱导PC12细胞中神经突增生,表现出高于ZLLal 500倍的效能。[1] MG-132 (10 μM) 通过抑制蛋白酶体介导的IκBα降解,有效抑制A549细胞中TNF-α诱导的NF-κB活化,白介素-8 (IL-8)基因转录,和IL-8蛋白质释放。[2] MG-132处理通过抑制26S蛋白酶体,有效诱导p53依赖性KIM-2细胞凋亡。[3]不同于BzLLLCOCHO或PS-341,MG-132处理对26S蛋白酶体的糜蛋白样(CT-L)和肽谷氨酰基肽水解(PGPH)活性抑制较弱,而相对于BzLLLCOCHO,MG-132能够更有效诱导多发性骨髓瘤细胞(U266 和OPM-2)的细胞凋亡。[4] MG-132 (1 μM)通过激活AP-1家族成员c-Fos和 c-Jun,使TRAIL抗性前列腺癌细胞敏感,这反过来抑制抗凋亡分子c-FLIP(L)。[5] MG-132显著增强肌醇六磷酸(IP6) 的作用以降低PC3 和DU145雄激素非依赖性前列腺癌(AIPCa)细胞系的细胞代谢活性。[6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LP-1 NIrQWJVCeG:ydH;zbZMhSXO|YYm= NI\BVXA{ODBibl2= NEL0XlUzPCCq NXHJW5ZqTE2VTx?= NUPaTpcxUW6mdXPld{BieG:ydH;zbZMh[nliaX7jdoVie2mwZzDjcIVifmWmIGDBVnAhdGW4ZXygZY5lKHKnZIXjbY5oKE2lbD2xJJBzd3SnaX6gcIV3\Wx? M3L0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
PC3 MmXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXDNlAh|ryP NHS0bXY1QCCq MoK4SG1UVw>? M{XsTmlEPTB;MD62JO69VQ>? NWDs[IQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFY2OzdpPkG2Olg3PTN5PD;hQi=>
PC12 M2PMOmZ2dmO2aX;uJGF{e2G7 MnrJNVAxKM7:TdMg M3r2SFI1KGh? MmPvSG1UVw>? MXzJcohq[mm2czC2MW9JTEFvIHHu[EBJOk9{LXnu[JVk\WRiY4n0c5RwgGmlaYT5 M2fGOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW4OFU1Lz5zN{G1PFQ2PDxxYU6=
LPS-stimulated RAW264.7 cells MVXGeY5kfGmxbjDBd5NigQ>? NXrwWlRPTE2VTx?= M4T2ZmlvcGmkaYTzJG5HNWujcIDhRkBFVkFiYnnu[Ilv\w>? M2[ybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEC3Nlc4Lz5zN{SwO|I4PzxxYU6=
OCI-Ly3 Mki3SpVv[3Srb36gRZN{[Xl? M{nXVFExKM7:TdMg MUW0JIg> MlfXSG1UVw>? NYGyTZdFUW6mdXPld{BpfW2jbjDJb4FxeGGEYXzwbIEhe3SjYnnsbZpifGmxbjD3bZRpKEWFNUCgc4YhOSEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB{NEGxN{c,OThyMkSxNVM9N2F-
A2780 cDDP MVvBdI9xfG:|aYOgRZN{[Xl? MVqyOEBp M1GybmROW09? NX\vdItWUW6mdXPld{BieG:ydH;zbZMh[nliaX7obYJqfGmwZzDQWGVPKGSnZ4Lh[IF1cW:w NVXabnJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PFQxOThpPkG3Olg1ODF6PD;hQi=>
COS-7 MYjDfZRwfG:6aXOgRZN{[Xl? NEfTcmoyOCEQvF2= MX3EUXNQ NV\EUnl5UUN3MEyxNEDPxE1? NEX2eI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4PFA6Pyd-MUiwPFgxQTd:L3G+
HuH-7 MmPqR5l1d3SxeHnjJGF{e2G7 M{TaTVExKM7:TR?= Mki4SG1UVw>? NETpV25KSzVyPEGwJO69VQ>? Mk[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyOEiwPVcoRjF6MEi4NFk4RC:jPh?=
Hepa-1c1c7 NFzmZlhHfW6ldHnvckBCe3OjeR?= MlrJNlUh|ryP MV:2JIg> MYjEUXNQ MWfJcoNz\WG|ZYOgUpJnOiCycn;0[YlvKGyndnXs NXf6fWNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzPVI2PDRpPkKwN|kzPTR2PD;hQi=>
IPC227F M333cGN6fG:2b4jpZ{BCe3OjeR?= M2Hvb|Eh|ryP NUnEXnZMPDhiaB?= NIm5N29FVVOR M3jYUmlEPTB;MD6wO|ch|ryP NIHT[nc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmyOFUzQCd-MkG5NlQ2Ojh:L3G+
Calu6 NGL5OZNHfW6ldHnvckBCe3OjeR?= NHf5T28yOCEQvF2= MkjBNVghcA>? MVnEUXNQ M1LDeHNq\26rZnnjZY51dHliYXPjeY12dGG2ZYOg[pJifGG6aX6gdJJm[3W{c3;y NWTRZ3E3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NFY1QDZpPkKzOVA3PDh4PD;hQi=>
IFN-gamma-induced RAW264.7 MnXBSpVv[3Srb36gRZN{[Xl? NYPOZnYzTE2VTx?= MXvJcohq[mm2czDubZRzcWNib4jp[IUheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE4xPzdizszN Ml\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{N{eyO|coRjJ{Mke3Nlc4RC:jPh?=
HEK-293 NXr2dWY1U2mwYYPlJGF{e2G7 M17MS|Ih[W6mIEKwJO69VQ>? NGPjc2szKGh? M4i3SGROW09? NXz0TI5tUW6qaXLpeJMhS2iWLVygZYN1cX[rdImge4l1cCCLQ{WwJI9nKDlibl2= NX\1UnpbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFA4QTBpPkKzOVQxPzlyPD;hQi=>
MCF10A NWfDO3NvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\ndIhSOSEQvF2= NFj5W2Y4OiCq NHf4UmNFVVOR MVjJR|UxRTBwMkmg{txO M2fHbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUWzNlA3Lz5{NEG1N|IxPjxxYU6=
MCF-7 NWn1eY9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVKxJO69VQ>? M1PqNFczKGh? Ml64SG1UVw>? NF;hbpFKSzVyPUCuNVMh|ryP NIXTWJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1N|IxPid-MkSxOVMzODZ:L3G+
HeLa MVnLbY5ie2ViQYPzZZk> NH6xcocyOCEQvF2= NV7Fc4Q3OSCq MnXlSG1UVw>? Ml61TY5lfWOnczDQRXJRKGOuZXH2ZYdmKGK7IHnubIljcXSrbnegdJJwe2Wjc3;t[UBi[3Srdnn0fS=> NUn2Oo81RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE5OzNpPkK0N|IyQDN|PD;hQi=>
MDA-MB-231 NEOxSVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDaNUDPxE1? MmnCO|IhcA>? MXTEUXNQ NH7ZenZKSzVyPUCuNVgh|ryP M2rtdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUWzNlA3Lz5{NEG1N|IxPjxxYU6=
293T NUnLRXVbS3m2b4TvfIlkKEG|c3H5 MlPQNVAh|ryP MoTJO|IhcA>? NWPYTYROTE2VTx?= MYLJR|UxRDJizszN Ml;mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4MkWwPFgoRjJ2NkK1NFg5RC:jPh?=
293T MnLKSpVv[3Srb36gRZN{[Xl? NInYTpkyOCEQvF2= NXLBXVJHOjRiaB?= M{\0RmROW09? MV7Nc4RmemG2ZXz5JIlv\HWlZYOgbIVifC2|aH;jby=> M1TpWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK1NFg5Lz5{NE[yOVA5QDxxYU6=
NCI-H929 NYSz[XNnS3m2b4TvfIlkKEG|c3H5 MUexJO69VQ>? NX;mT2xCPzJiaB?= MXPEUXNQ M3TnemlEPTB;MD6xO{DPxE1? NVf4b5lTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlUxQDhpPkK0OlI2ODh6PD;hQi=>
A-549 MWPDfZRwfG:6aXOgRZN{[Xl? NXzzRZh6PDBizszN NH7WSpM1QCCq MVLEUXNQ NE\xS3dKSzVyPEGwJO69VQ>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7N{S5Okc,OjR4OUe0PVY9N2F-
MCF-7 NHvDZmxEgXSxdH;4bYMhSXO|YYm= NXfRRpRWPDBizszN MXS0PEBp NWfHNIJUTE2VTx?= MoGyTWM2OD15LkOg{txO MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7N{S5Okc,OjR4OUe0PVY9N2F-
SW-480 NIfEdJhEgXSxdH;4bYMhSXO|YYm= MYi0NEDPxE1? MnThOFghcA>? NVHJXWlYTE2VTx?= NFXBSGxKSzVyPUSg{txO NELvTZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
HL-60 NGfVZWtEgXSxdH;4bYMhSXO|YYm= NXLpW4p5PDBizszN M1HiXFQ5KGh? NVXofnV[TE2VTx?= M2rrR2lEPTB:MUCg{txO M1TEZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm3OFk3Lz5{NE[5O|Q6PjxxYU6=
SMMC-7721 MkHjR5l1d3SxeHnjJGF{e2G7 MlrROFAh|ryP MUm0PEBp Mmr5SG1UVw>? NIrLW5ZKSzVyPUeuNUDPxE1? NIjvN3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
LPS-stimulated RAW264.7 cells MoX1SpVv[3Srb36gRZN{[Xl? M3HPNlI2KM7:TR?= NGrhfG5FVVOR MV7Jcohq[mm2czDubZRzcWNib4jp[IUheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE4yKM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7N{S5Okc,OjR4OUe0PVY9N2F-
lymphoblastoid NUHMfmdJTnWwY4Tpc44h[XO|YYm= MVHJR|UxKD1iMD6wNFIh|ryP M1jOT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUm2PVg4Lz5zN{m5Olk5PzxxYU6=
LCL M1m4UmZ2dmO2aX;uJIF{e2G7 NUHVNVNqOTJiaB?= M4ruS2lEPTBiPTCwMlAxOiEQvF2= M1rKdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNki3OlA6Lz5{ME[4O|YxQTxxYU6=
HL-60 MXXGeY5kfGmxbjDhd5NigQ>? M3v6OGlEPTBiPTCwMlA3QCEQvF2= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6ME[yN{c,OTV5OEC2NlM9N2F-
IPC227F NXTSSpY3TnWwY4Tpc44h[XO|YYm= MXGxJIg> MYnJR|UxKD1iMD6wO|ch|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ{MEm4PEc,OTZ{MkC5PFg9N2F-
RAW264.7 M4jUbmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZZN{[Xl? NF3YSmcyQCCq MlvnTWM2OCB;IECuNFgh|ryP NUPsWWtzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NlA3PzdpPkKyOlIxPjd5PD;hQi=>
RAW264.7 NFnNUGZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIF{e2G7 MYOxPEBp NV;BVYJQUUN3MDC9JFAvOSEQvF2= M1;ic|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG5PFcyLz5{M{ixPVg4OTxxYU6=
RAW264.7 M1fXZ2Z2dmO2aX;uJIF{e2G7 NUGzTXBUUUN3MDC9JFAvOSEQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV7N{i5OEc,OjR3OUe4PVQ9N2F-
RAW264.7 MkD2SpVv[3Srb36gZZN{[Xl? MlTPNVghcA>? NWTOSFliUUN3MDC9JFAvOSEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzOUiwPUc,OjR{MUm4NFk9N2F-
RAW264.7 NFXlU5BHfW6ldHnvckBie3OjeR?= M{THcVE5KGh? MVrJR|UxKD1iMD6xOUDPxE1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{N{[2PEc,OjN|Mke2Olg9N2F-
RAW264.7 NF;aR5dHfW6ldHnvckBie3OjeR?= MkDoNVghcA>? NVLvZlVoUUN3MDC9JFAvOTVizszN MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3N{CxPUc,OjZ5NUewNVk9N2F-
RAW264.7 NFjodmZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIF{e2G7 NXSyVGF{OThiaB?= NYPoRXhuUUN3MDC9JFAvOThizszN NVe2fXIxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkeyNFMzQTFpPkK3NlA{OjlzPD;hQi=>
RAW264.7 MYXBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHHzd4F6 MWqxJIg> NEXRXZFKSzVyIE2gNE4zKM7:TR?= NEmxR2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUOzPFI3OCd-MkmzN|gzPjB:L3G+
MDA-MB-231 MojER5l1d3SxeHnjbZR6KGG|c3H5 NEHqVWxKSzVyIE2gNE4zPSEQvF2= MkDnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3OUiwNVgoRjF6NUm4NFE5RC:jPh?=
MCF7 MWPDfZRwfG:6aXPpeJkh[XO|YYm= MUHJR|UxKD1iMD6zJO69VQ>? NHH2UoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW5PFAyQCd-MUi1PVgxOTh:L3G+
RAW264.7 NXi4dG14TnWwY4Tpc44h[XO|YYm= NHXjVIUyQCCq NYDFe4lEUUN3MDC9JFAvOzVizszN NHLm[VE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC0OFg1Pyd-MkGwOFQ5PDd:L3G+
RAW264.7 NEDPV|ZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIF{e2G7 M4Pyd2lEPTBiPTCwMlQ{QSEQvF2= M4PGfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNU[xNFYxLz5{MUW2NVA3ODxxYU6=
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NCI-H2196 MnzUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfBfItKSzVyPU[xMlI{OSEQvF2= MmPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
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RPMI-8402 M{TjXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTV6Lkm1NlYh|ryP NGjxN409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PDl{NT:nQnNCVkeHUkyvZV4>
NCI-H1395 NYfRNpQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7pOlZsUUN3ME21PE45OTd{IN88US=> MmrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
KP-N-RT-BM-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\hZokzUUN3ME21Ok43PjN6IN88US=> MmXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
SW962 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTV2LkizOVch|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2OFkzPS9pPmPBUmdGWjxxYU6=
TC-YIK NGfvXVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;FTmNQUUN3ME21NE4xOzZ|IN88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2OFkzPS9pPmPBUmdGWjxxYU6=
A3-KAW MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\6d2lEPTB;NEmuPFA3OSEQvF2= NVr6ZpdvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjR7MkWvK|5USU6JRWK8M4E,
HT M3:5d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPBTWM2OD12NT63NFA4KM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2OFkzPS9pPmPBUmdGWjxxYU6=
SW954 MofTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoGxTWM2OD12Nz60NVI5KM7:TR?= NEjQfG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PDl{NT:nQnNCVkeHUkyvZV4>
CW-2 MnvwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3DTWM2OD12ND64N|QzKM7:TR?= M2PpbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
EoL-1- MnX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPYS5hqUUN3ME20OE4yPTZ6IN88US=> M4K3b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
MPP-89 NEnpbYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTlfFJlUUN3ME20Nk42QDd3IN88US=> M3PuNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
MV-4-11 NE\6WnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFTYVW1KSzVyPUSyMlkxPjdizszN M4f0WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
SBC-1 NFTqPZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTR{LkG4NFYh|ryP M1POPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
MLMA MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjETWM2OD12MT63OVk2KM7:TR?= MmfQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
DEL Mnf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUW3S3lTUUN3ME20NU41PDB3IN88US=> NITseWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PDl{NT:nQnNCVkeHUkyvZV4>
LB996-RCC NVO1S3N7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TJSGlEPTB;NECuPFcyPiEQvF2= M37XWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
NCI-H64 NVr2S5dtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkK4TWM2OD12MD6wPVQ5KM7:TR?= MnvQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
MHH-PREB-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTN7LkezPFgh|ryP NI\N[GM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PDl{NT:nQnNCVkeHUkyvZV4>
NCI-SNU-16 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\NSZhkUUN3ME2zPE42PjV|IN88US=> NUPBU2g6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjR7MkWvK|5USU6JRWK8M4E,
LC-2-ad MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7HTWM2OD1|OT60N|M4KM7:TR?= M1LWSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
RCC10RGB NVixVIxVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnf0TWM2OD1|OD6yNFg{KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2OFkzPS9pPmPBUmdGWjxxYU6=
NCI-H1299 MnjnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTN6LkG2NFMh|ryP Mk\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlQ6OjVxJ{7TRW5ITVJ:L3G+
K-562 NYHCfIdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DZOGlEPTB;M{euNFg3PCEQvF2= NIKwV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PDl{NT:nQnNCVkeHUkyvZV4>
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CW-2 M3HRWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTR6Mz62NFkh|ryP M4jq[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY1QTJ3Lze+V2FPT0WUPD;hQi=>
COLO-800 MnfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLPR5RKSzVyPUWyOU41OTNizszN NYfMbYFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjR7MkWvK|5USU6JRWK8M4E,
EW-12 MlXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3XfJp7UUN3ME21OVcvQDR5IN88US=> NYLIZnBPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjR7MkWvK|5USU6JRWK8M4E,
EW-18 M{XET2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTZ5Mj62OlUh|ryP MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2OFkzPS9pPmPBUmdGWjxxYU6=
Assay
Methods Test Index PMID
Western Blot HO-1 Nrf2 CgA ASCL1 Cleaved-PARP Cleaved-caspase3 Ac-histone H3 Bcr-Abl 23604711 19765735 26722260
Immunofluorescence AGS3 / Vimentin C2GnT-M / NMIIA Bak 20065032 22525330 20557369
Growth inhibition assay cell proliferation Cell viability Apoptosis assay 19765735 26137056 20557369
ELISA IL-1beta IL-8 22606244 11160671
体内研究

MG-132给药有效恢复mdx小鼠骨骼肌纤维中肌萎缩蛋白,β-营养不良聚糖蛋白,α-营养不良聚糖蛋白,和α-肌聚糖蛋白的表达水平和血浆膜定位,减少肌肉膜损伤,并改善肌肉萎缩症的组织病理学信号。[7] MG-132治疗通过下调肌肉特异性泛素连接酶atrogin-1/MAFbx 和MuRF-1 mRNA,显著减少小鼠体内固定化诱导的骨骼肌萎缩。[8]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

  • MG-132对20S蛋白酶体抑制活性的测定:

    20S蛋白酶体抑制试验的反应混合物包含0.1 M Tris-乙酸,pH 7.0,20S 蛋白酶体,MG-132,和25 μM底物,溶解于二甲基亚砜,终体积为1 mL。在37 °C下培养15分钟后,加入pH 9.0的0.1 mL 10% SDS 和0.9 mL 0.1M Tris 乙酸盐停止反应。测量反应产物的荧光。为测定对20S蛋白酶体的IC50,试验混合物中包含不同浓度的MG-132。

细胞实验:

[3]

  • Cell lines: KIM-2,HC11,和 ES
  • Concentrations: 溶解于DMSO,终浓度为~25 μM
  • Incubation Time: 24 和 48小时
  • Method:

    细胞在不同浓度的MG-132下暴露24,和48小时。上清液和单层细胞通过离心采集,并固定在含70%乙醇的PBS中,用吖啶橙着色。等体积细胞和吖啶橙(5 mg/mL的PBS溶液)在显微镜载玻片上固定,并通过荧光显微镜检测。对于膜联蛋白V分析,细胞通过离心采集,并用膜联蛋白V和碘化丙啶着色。对于细胞分析,细胞在PBS中于室温下再水化10分钟,随后用碘化丙啶(5 mg/mL)着色。所有样品使用Coulter Epics XL流式细胞分析仪分析。

动物实验:

[7]

  • Animal Models: 雄性 mdx (C57BL/10ScSn DMD mdx) 小鼠
  • Dosages: ~10 μg/kg/day
  • Administration: 注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O

2.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 475.62
化学式

C26H41N3O5

CAS号 1211877-36-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

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常见问题及建议解决方法

问题 1:
I want to know whether it contains EDTA or other chelating agent in this product(S2619)?

回答:
Our S2619 MG-132 doesn't contain EDTA or other chelating agent.

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