Melatonin (NSC 113928)

For research use only. Not for use in humans.

目录号:S1204 别名: NSC 56423, N-Acetyl-5-methoxytryptamine 中文名称:褪黑素

Melatonin (NSC 113928) Chemical Structure

CAS No. 73-31-4

Melatonin (NSC 113928, NSC 56423, N-Acetyl-5-methoxytryptamine)是一种 MT receptor 激动剂激素, 用作膳食补充品。Melatonin 是一种选择性的 ATF-6 的抑制剂并可下调 COX-2。Melatonin 可增强线粒体自噬并调节凋亡和自噬的稳态。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 732.95 现货
RMB 804.46 现货
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客户使用Selleck生产的Melatonin (NSC 113928)发表文献19篇:

产品安全说明书

MT Receptor抑制剂选择性比较

生物活性

产品描述 Melatonin (NSC 113928, NSC 56423, N-Acetyl-5-methoxytryptamine)是一种 MT receptor 激动剂激素, 用作膳食补充品。Melatonin 是一种选择性的 ATF-6 的抑制剂并可下调 COX-2。Melatonin 可增强线粒体自噬并调节凋亡和自噬的稳态。
靶点
melatonin receptor [1]
()
体外研究

Melatonin与高毒性羟基自由基相互作用,数率常数等于其他高效羟基自由基清除剂。Melatonin被报到能够中和过氧化氢,单线态氧,过氧亚硝基阴离子,一氧化氮和次氯酸。[1] Melatonin被认为能够清除高毒性羟基自由基,过氧亚硝酸盐阴离子,可能也作用于氢过氧自由基。据报道,Melatonin清除超氧阴离子自由基,并且能够淬灭单线态氧。Melatonin刺激超氧化物歧化酶的mRNA水平,并活化谷胱甘肽过氧化物酶,谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶(都为抗氧化酶),从而增加其抗氧化能力。[2] 在体外,Melatonin在无细胞体系中被证实能够直接清除H2O2,单线态氧(1O2)和一氧化氮(NO*),几乎或完全没有清除超氧阴离子自由基(O2*-)的能力。Melatonin也能够直接作用于过氧亚硝基阴离子(ONOO-)和/或过氧亚硝酸(ONOOH),或该分子的活化形式,ONOOH*,使它们失去毒性。Melatonin作为一种直接自由基清除剂,具有清除活性氧和活性氮自由基毒性的能力。[3] Melatonin抑制大部分检测细胞中的cAMP积累,但至今为止,对其它信使吲哚基的作用仅在一种类型的细胞或组织中经常观察到。Melatonin也能够调节转录因子,即cAMP响应元件结合蛋白的磷酸化和c-Fos的表达。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO MWnGeY5kfGmxbjDhd5NigQ>? NXXpNFN4UW6qaXLpeIlwdiCxZjD0bIUhOi2dMUK1TX0uKGmxZH;t[YxifG:waX6gZolv\GmwZzD0c{BO\WyjdH;ubY4hemWlZYD0c5IhfHmyZTCxRUBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDLbV0xNjByMEC4{txO NYmzfGM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUSzOVg6OCd-OUSzOVg6ODxxYU6=
CHO NUfMflJDTnWwY4Tpc44h[XO|YYm= M{j1OGlvcGmkaYTpc44hd2ZidHjlJFIuYzF{NVndMUBqd2SxbXXsZZRwdmmwIHLpcoRqdmdidH:gUYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVKg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xODBzMt88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86PDN3OEmwK|46PDN3OEmwQE9iRg>?
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NIH 3T3 NEDncJZHfW6ldHnvckBie3OjeR?= M2facmJqdmSrbnegZYZncW6rdImgeI94[XKmczDy[YNwdWKrbnHueEBpfW2jbjDt[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQjDlfJBz\XO|ZXSgbY4hVkmKIEPUN{Bk\WyuczD1d4lv\yB{LWuxNlFKZWmxZH;t[YxifG:waX6gdoFlcW:uaXfhcoQh[mmwZHnu[{Bie3OjeTygT4k:OC5yMECzN:69VQ>? MmLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB5M{e3N|goRjFyN{O3O|M5RC:jPh?=
NIH 3T3 M2TtUmZ2dmO2aX;uJIF{e2G7 NGHPUm9DcW6maX7nJIFn\mmwaYT5JJRwf2G{ZIOgdoVkd22kaX7hcpQhcHWvYX6gcYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGg[ZhxemW|c3XkJIlvKE6LSDCzWFMh[2WubIOgeZNqdmdiMj3bNVIyUV2rb3TvcYVt[XSxbnnuJJJi\GmxbHnnZY5lKGKrbnTpcoch[XO|YYmsJGtqRTBwMECwOlbPxE1? NWLDUIRwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3N|c4OzhpPkGwO|M4PzN6PD;hQi=>
HEK293 MVXGeY5kfGmxbjDhd5NigQ>? MkXYRolv\GmwZzDh[oZqdmm2eTDmc5IhcHWvYX6gcYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGsJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgLFIuYzF{NVndbY9ld22nbHH0c45qdiCrczD1d4VlKGG|IILh[IlwdGmpYX7kLUwhU2l;MD6wNFAz|ryP MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTB4M{[wNkc,OTFyNkO2NFI9N2F-
HEK293 NYe3O|U1TnWwY4Tpc44h[XO|YYm= NX3zSI1ESmmwZHnu[{Bi\m[rbnn0fUBnd3JibXXsZZRwdmmwIILlZ4VxfG:{IIT5dIUhOUJuIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiKEKtX|EzPUmfaX;kc41mdGG2b37pckBqeyC3c3XkJIF{KHKjZHnvcIlo[W6mKTygT4k:OC5yMEC1N:69VQ>? M324c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzME[zOlAzLz5zMUC2N|YxOjxxYU6=
NIH 3T3 Mk\3SpVv[3Srb36gZZN{[Xl? NYDRWmw2SmmwZHnu[{Bi\m[rbnn0fUB1d3ejcnTzJI1mdGG2b37pckBz\WOncITvdkB1gXCnIEHCJJN1[WKueTDlfJBz\XO|ZXSgbY4hVkmKM2SzJJJifCCoaXLyc4Jt[XO2IHPlcIx{KHW|aX7nJFIuYzF{NVndbY9ld22nbHH0c45qdixiS3m9NE4xODB{OEG4{txO NYjPOGRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1NlAyQThpPkGxOVIxOTl6PD;hQi=>
NIH 3T3 NEXmdYRHfW6ldHnvckBie3OjeR?= NF\CXpBDcW6maX7nJIFn\mmwaYT5JJRwf2G{ZIOgcYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGgd5Ri[my7IHX4dJJme3OnZDDpckBPUUh|VEOgdoF1KG[rYoLvZoxie3RiY3XscJMhfXOrbnegNk1cOTJ3SW3pc4RwdWWuYYTvcolvKChzMECgdG0qKGG|IILh[IlwdGmpYX7kMEBMcT1yLkCwNFI5QDUQvF2= M1jOOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNUKwNVk5Lz5zMUWyNFE6QDxxYU6=
HEK293 Mn22SpVv[3Srb36gZZN{[Xl? M4DvcWlvcGmkaYTpc44hd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIJqdmSrbnegeI8hcHWvYX6gUYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMECxNu69VQ>? M2P3WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[wOFk4Lz5zMUm2NFQ6PzxxYU6=
HEK293 M2e5e2Z2dmO2aX;uJIF{e2G7 NX\pb4FiUW6qaXLpeIlwdiCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h[mmwZHnu[{B1dyCqdX3hckBO\WyjdH;ubY4hemWlZYD0c5IhfHmyZTCxRkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkCwNFMy|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTl4MES5O{c,OTF7NkC0PVc9N2F-
HEK293 NH\4cHNHfW6ldHnvckBie3OjeR?= MmP0Rolv\GmwZzDh[oZqdmm2eTDvckBpfW2jbjDt[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQjDzeIFjdHlidILhcpNn\WO2ZXSgbY4hcHWvYX6g[Y1jenmxbnnjJItq\G6neTCoTGVMKDJ7MzmgZ4VtdHNidYPpcochOi2dMUK1TX1qd2SxbXXsZZRwdmmwIHHzJJJi\GmxbHnnZY5lNixiS3m9NE4xODB|Md88US=> NIrsbWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkC2NVg5OSd-MUKwOlE5QDF:L3G+
CHO NXrZWZNCTnWwY4Tpc44h[XO|YYm= NFjTS3RKdnS{aX7zbYMh[WO2aY\peJkh[XRiaIXtZY4hVWWuYYTvcolvKHKnY3XweI9zKHS7cHWgNWIh\X[jbIXheIVlKG:wIGuzOXNeT1SSLXfhcY1iNVNuIHLpcoRqdmdiaX6gR4hqdmW|ZTDoZY1{fGW{IH;2ZZJq[W5iKFPIU{kh[2WubIOsJGVEPTB;MD6wNFA1P87:TR?= M1:xVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{ME[xPFgyLz5zMkC2NVg5OTxxYU6=
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CHO MX\GeY5kfGmxbjDhd5NigQ>? NETyZndC[mmuaYT5JJRwKGmwaHnibZQhOi2dMUK1TX1qd2SxbXXsZZRwdmmwIIPw[YNq\mmlIHLpcoRqdmdidH:gbJVu[W5ibXXsZZRwdmmwIILlZ4VxfG:{IIT5dIUhOUFiKF3UNUkh\XiycnXzd4VlKGmwIFPIU{Bk\Wyucz6sJGtqRTBwMECwNFgzO87:TR?= NIjHbGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkKxN|A3Oid-MUKyNVMxPjJ:L3G+
CHO MV;GeY5kfGmxbjDhd5NigQ>? NULJeYlkSmmwZHnu[{Bi\m[rbnn0fUBi\2GrboP0JIh2dWGwIF3lcIF1d26rbjDy[YNmeHSxcjD0fZBmKDGDIDjNWFEqKGmwIFPIU{Bk\WyuczygT4k:OC5yMECwPFI{|ryP NFv6PZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkKxN|A3Oyd-MUKyNVMxPjN:L3G+
HEK293 NWHaUo9VTnWwY4Tpc44h[XO|YYm= MoftRolv\GmwZzDh[oZqdmm2eTDmc5IhcHWvYX6gUYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGgd5Ri[my7IITyZY5{\mWldHXkJIlvKGi3bXHuJIVu[nK7b37pZ{BscWSwZYmgZ4VtdHNiKFjFT{AzQTNrIIXzbY5oKDJvW{GyOWlecW:mb33lcIF1d26rbjDhd{Bz[WSrb3zp[4Fv\CxiS3m9NE4xODBzNN88US=> M4G0VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NkS2NFIzLz5zMk[0OlAzOjxxYU6=
HEK293 NWD3eXNKTnWwY4Tpc44h[XO|YYm= MXfCbY5lcW6pIHHm[olvcXS7IH\vdkBpfW2jbjDN[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQjDzeIFjdHlidILhcpNn\WO2ZXSgbY4hcHWvYX6g[Y1jenmxbnnjJItq\G6neTDj[YxteyBqSFXLJFI6OylidYPpcochOi2dMUK1TX1qd2SxbXXsZZRwdmmwIHHzJJJi\GmxbHnnZY5lNCCNaU2wMlAxODRzzszN MofaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4NE[wNlIoRjF{NkS2NFIzRC:jPh?=
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NIH3T3 NH;vdWhHfW6ldHnvckBie3OjeR?= NHHrNJVKdmirYnn0bY9vKG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBjcW6maX7nJJRwKG2nbXLyZY5mKHC{ZYDhdoF1cW:wczDv[kBPUUh|VEOgZ4VtdHNic4ThZox6KGW6cILld5NqdmdiaIXtZY4hVWWuYYTvcolvKHKnY3XweI9zKHS7cHWgNWEtKEurPUCuNFAxO87:TR?= M1fveFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3M{iwNlE5Lz5zNUO4NFIyQDxxYU6=
NIH3T3 NXjBUHI4TnWwY4Tpc44h[XO|YYm= MUTJcohq[mm2aX;uJI9nKDJvW{GyOWlecW:mb33lcIF1d26rbjDibY5lcW6pIITvJI1mdWK{YX7lJJBz\XCjcnH0bY9veyCxZjDOTWg{XDNiY3XscJMhe3SjYnz5JIV5eHKnc4PpcochcHWvYX6gUYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVKsJGtqRTBwMECwO:69VQ>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN6MEKxPEc,OTV|OECyNVg9N2F-
NIH3T3 MVvGeY5kfGmxbjDhd5NigQ>? M3;DZ2JqdmSrbnegZYZncW6rdImgZYdicW6|dDDN[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQTDzeIFjdHliZYjwdoV{e2WmIHnuJG5KUDOWMzDj[YxteyC3c3nu[{AzNVtzMkXJYYlw\G:vZXzheI9vcW5uIFvpQVAvODByM988US=> NYjRNnRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVM{QDRpPkG1O|E{Ozh2PD;hQi=>
NIH3T3 NV\GO4o2TnWwY4Tpc44h[XO|YYm= M13rTGJqdmSrbnegZYZncW6rdImgZYdicW6|dDDN[YxifG:waX6gdoVk\XC2b4KgeJlx\SBzQjDzeIFjdHliZYjwdoV{e2WmIHnuJG5KUDOWMzDj[YxteyC3c3nu[{AzNVtzMkXJYYlw\G:vZXzheI9vcW5uIFvpQVAvODByN988US=> NFXsNYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexN|M5PCd-MUW3NVM{QDR:L3G+
NIH3T3 MUnGeY5kfGmxbjDhd5NigQ>? MYTJcohq[mm2aX;uJI9nKDJvW{GyOWlecW:mb33lcIF1d26rbjDibY5lcW6pIITvJIh2dWGwIH3lcIF1d26rbjDy[YNmeHSxcjD0fZBmKDGEIDjNWFIqKGW6cILld5Nm\CCrbjDOTWg{XDNicnH0JIZq[nKxYnzhd5Qh[2WubIOgMEBMcT1yLkCwNFI5OTkQvF2= M2L3VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUSzOFc5Lz5zNUm0N|Q4QDxxYU6=
NIH3T3 Mo\mSpVv[3Srb36gZZN{[Xl? M4TKSmlvcGmkaYTpc44hd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIJqdmSrbnegeI8hcHWvYX6gcYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVGgLG1VOSliZYjwdoV{e2WmIHnuJG5KUDOWMzDyZZQh\mmkcn;icIF{fCClZXzsd{whU2l;MD6wNFAzQDh2zszN NWC0RodsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OFM1PzhpPkG1PVQ{PDd6PD;hQi=>
NIH3T3 M3TSZ2Z2dmO2aX;uJIF{e2G7 MVjEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hemWlb33ibY5idnRiTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCQSVizWFMh[2WubIOsJGtqRTBwMECwN|XPxE1? M4\NblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{W5NFk1Lz5zNke1PVA6PDxxYU6=
NIH3T3 Ml3rSpVv[3Srb36gZZN{[Xl? M2HaVmRqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDNWFEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIF7JTFNVOyClZXzsd{whU2l;MD6wNFA{Qc7:TR?= NXL6bHBvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3OVkxQTRpPkG2O|U6ODl2PD;hQi=>
NIH3T3 M1W1XWZ2dmO2aX;uJIF{e2G7 MYSxJI5O MmK4RYN1cX[rdImgZZQhVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBPUUh|VEOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCob4Lzb49tcW5vc4TpcZVt[XSnZDDjRW1RKHO7boTo[ZNqeyCjdDCxJI5O NXvudY5nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3OVkxQTRpPkG2O|U6ODl2PD;hQi=>
NIH3T3 MYXGeY5kfGmxbjDhd5NigQ>? MkfpSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hVkmKM2SzJINmdGy|LDDLbV0xNjByMEK1O:69VQ>? MkXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNEm4OlkoRjF5MUS5PFY6RC:jPh?=
NIH3T3 MYjGeY5kfGmxbjDhd5NigQ>? M1:4S2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE6LSEPUN{Bk\WyuczygT4k:OC5yMECzNlM3|ryP MkjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNEm4OlkoRjF5MUS5PFY6RC:jPh?=
NIH 3T3 M3\aTWZ2dmO2aX;uJIF{e2G7 NG\SbVRFcXOybHHj[Y1mdnRib3[gNnsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDNWFIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIF7JTEA{XDNiY3XscJMtKEurPUCuNFAxOzV2ON88US=> NUnkRVcxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOFY5PTlpPkG3N|Q3QDV7PD;hQi=>
NIH 3T3 NV\sXFBvTnWwY4Tpc44h[XO|YYm= NFX6SGRFcXOybHHj[Y1mdnRib3[gNnsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDNWFEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIF7JTEA{XDNiY3XscJMtKEurPUCuNFAxOzh7zszN MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2Nki1PUc,OTd|NE[4OVk9N2F-
NIH3T3 M2rMOWZ2dmO2aX;uJIF{e2G7 MVLEbZNxdGGlZX3lcpQhd2ZiMmuxNlVKZWmxZH;t[YxifG:waX6g[pJwdSCqdX3hckBz\WOxbXLpcoFvfCCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE6LSEPUN{Bk\WyuczygT4k:OC5yMECyOlPPxE1? NILkXIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S1PVcyOSd-MUe0OVk4OTF:L3G+
NIH3T3 NEPjOGZHfW6ldHnvckBie3OjeR?= MWrEbZNxdGGlZX3lcpQhd2ZiMmuxNlVKZWmxZH;t[YxifG:waX6g[pJwdSCqdX3hckBz\WOxbXLpcoFvfCCPVEKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE6LSEPUN{Bk\WyuczygT4k:OC5yMECzN|g5|ryP NXHUbIl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OVk4OTFpPkG3OFU6PzFzPD;hQi=>
NIH3T3 NHq5NIZHfW6ldHnvckBie3OjeR?= M2joSmRqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiClbH;u[YQhVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBz[XRiTlnIN3Q{KGOnbHzzMEBMcT1yLkCwNFLPxE1? MmntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OEG5NFQoRjF5NEixPVA1RC:jPh?=
NIH3T3 NF3BOVFHfW6ldHnvckBie3OjeR?= MkO1SIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKGOub37l[EBOXDJicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJJJifCCQSVizWFMh[2WubIOsJGtqRTBwMECwN:69VQ>? NUPp[IFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0PFE6ODRpPkG3OFgyQTB2PD;hQi=>
NIH3T3 MoXlSpVv[3Srb36gZZN{[Xl? NFrzSJBFcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iY3zvcoVlKE2WMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hemG2IF7JTFNVOyClZXzsd{whU2l;MD6wNFAyPDF|zszN NFjsR2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC1NlMyPCd-MUiwOVI{OTR:L3G+
NIH3T3 M2LYe2Z2dmO2aX;uJIF{e2G7 M37TSGRqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiClbH;u[YQhVVR{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBz[XRiTlnIN3Q{KGOnbHzzMEBMcT1yLkCwNFI{QToQvF2= NVH4UmpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwOVI{OTRpPkG4NFUzOzF2PD;hQi=>
HEK293 NFzuZ3BHfW6ldHnvckBie3OjeR?= MWnEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVA:OC5yMECy{txO MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN5MkG4NUc,OTh|N{KxPFE9N2F-
HEK293 Mke2SpVv[3Srb36gZZN{[Xl? NXTWOGhoTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBKSzVyPUCuNFAxPTQQvF2= NXewbotyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizO|IyQDFpPkG4N|czOThzPD;hQi=>
CHO NV:3[3NyTnWwY4Tpc44h[XO|YYm= MUXEbZNxdGGlZX3lcpQhd2ZiW{GyOWlecW:mb33lcIF1d26rbjDmdo9uKE2WMz;RVlIhdWWuYYTvcolvKGKrbnTpcoche2m2ZTDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCLQ{WwQVAvODZ2NUhOwG0> M4f3VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NECwN|M2Lz5zOESwNFM{PTxxYU6=
CHO M3q4XWZ2dmO2aX;uJIF{e2G7 MWfEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hdWWuYYTvcolvKE2WMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlAxODV{Nd88US=> MoP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NUe5PFAoRjF6NkW3PVgxRC:jPh?=
CHO M37vWmZ2dmO2aX;uJIF{e2G7 MmDESIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKG2nbHH0c45qdiCPVEKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xODB5NEJOwG0> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ3N{m4NEc,OTh4NUe5PFA9N2F-
HEK293 MYHGeY5kfGmxbjDhd5NigQ>? MUjEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODByMUVOwG0> NXPsd2drRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi3O|g6PDNpPkG4O|c5QTR|PD;hQi=>
CHO NX33eodnTnWwY4Tpc44h[XO|YYm= MXXEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hVVR{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUCuNFAxPDIQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd5OEm0N{c,OTh5N{i5OFM9N2F-
CHO MVXGeY5kfGmxbjDhd5NigQ>? MYrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHL5JHs{PVOfR2TQ[4FudWGVIHLpcoRqdmdiYYPzZZktKEWFNUC9NE4xODB2Od88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd5OEm0N{c,OTh5N{i5OFM9N2F-
CHO NFfpR2NHfW6ldHnvckBie3OjeR?= NHfOUJVFcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODByNEW3Ne69VQ>? MnnGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzOUOxOlAoRjF7MUmzNVYxRC:jPh?=
CHO NVTaS4V{TnWwY4Tpc44h[XO|YYm= MonOSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlAxODl3Nd88US=> NGPnN2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG5N|E3OCd-MUmxPVMyPjB:L3G+
CHO M3e4UmZ2dmO2aX;uJIF{e2G7 NEnqSGpC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJG1VOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KHO2aX31cIF1cW:wIH;mJHs{PVOfR2TQ[4FudWGVIHLpcoRqdmduIFXDOVA:OC5yMEC0Pe69VQ>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN{OUOyN{c,OTl|MkmzNlM9N2F-
CHO NVHHUWFHTnWwY4Tpc44h[XO|YYm= MWrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHzd4V{e2WmIHHzJJN1cW23bHH0bY9vKG:oIGuzOXNeT1SSZ3HtcYFUKGKrbnTpcoctKEWFNUC9NE4xODJ{NN88US=> NHuwbVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOyPVMzOyd-MUmzNlk{OjN:L3G+
CHO NEjw[GFHfW6ldHnvckBie3OjeR?= NWC2O|hJTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHL5JIdidW2jIHPveY51cW6pLDDLbV0xNjByMES1O|HPxE1? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR5M{i0PEc,OTl2N{O4OFg9N2F-
CHO MXPGeY5kfGmxbjDhd5NigQ>? NU\oWJBvTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHL5JIdidW2jIHPveY51cW6pLDDLbV0xNjByMEm1Oe69VQ>? NIfsXVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS3N|g1QCd-MUm0O|M5PDh:L3G+
CHO MUDGeY5kfGmxbjDhd5NigQ>? NHmyZ2s3OCCvaX7z NHmwUZZFcXOybHHj[Y1mdnRib3[gX|NJZW2nbHH0c45qdiCocn;tJIh2dWGwIH3lcIF1d26rbjDNWFEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDh[pRmeiB4MDDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurPUCuNFAxOjl4zszN M{OyfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMkK3PFc5Lz5{MEKyO|g4QDxxYU6=
CHO MWrGeY5kfGmxbjDhd5NigQ>? NEm2R3o3OCCvaX7z NGfH[21FcXOybHHj[Y1mdnRib3[gX|NJZW2nbHH0c45qdiCocn;tJIh2dWGwIH3lcIF1d26rbjDNWFIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDh[pRmeiB4MDDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurPUCuNFAxPDJ7zszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODJ{N{i3PEc,OjB{Mke4O|g9N2F-
CHO MYjGeY5kfGmxbjDhd5NigQ>? MlOwRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDNWFIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDifUBcOzWVXVfUVIdidW2jIHLpcoRqdmdiYYPzZZktKEWFNUC9NE4xODB2Od88US=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR2NE[xNEc,OjB2NES2NVA9N2F-
CHO MWXGeY5kfGmxbjDhd5NigQ>? NVjSXJFYSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBOXDFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{BjgSCdM{XTYWdVWGejbX3hJIJqdmSrbnegZZN{[XluIFXDOVA:OC5yMEKy{txO M1z3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNES0OlExLz5{MES0OFYyODxxYU6=
NIH3T3 MVHGeY5kfGmxbjDhd5NigQ>? M2PxUFkxKG2rboO= MlvGSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZWmxZH;t[YxifG:waX6g[pJwdSCqdX3hckBkdG:wZXSgUXQyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDyZZQhVkmKM2SzJINmdGy|IHHmeIVzKDlyIH3pcpMtKEurPUCuNFAxOzNzMd88US=> M{HiXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNke0N|c{Lz5{ME[3OFM4OzxxYU6=
NIH3T3 MV;GeY5kfGmxbjDhd5NigQ>? NHz3b4E6OCCvaX7z NXzMSnh{TGm|cHzhZ4Vu\W62IH;mJHsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiClbH;u[YQhVVR{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBz[XRiTlnIN3Q{KGOnbHzzJIFnfGW{IEmwJI1qdnNuIFvpQVAvODByNkG2Ou69VQ>? NU[xUXp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2O|Q{PzNpPkKwOlc1Ozd|PD;hQi=>
CHO-Galpha16 MVjGeY5kfGmxbjDhd5NigQ>? MkL3NlAhdWmwcx?= MY\CbY5lcW6pIHHm[olvcXS7IITvJJJifCCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3HZYxxcGFzNjDj[YxteyCjc4Pld5Nm\CCjczDDZVIsKG2xYnnsbZpifGmxbjDh[pRmeiB{MDDtbY5{KGK7IF\MTXBTKGG|c3H5MEBGSzVyPUCuNFAxPTcQvF2= M{K0NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkO3OlQ1Lz5{MUKzO|Y1PDxxYU6=
CHO-Galpha16 MnjiSpVv[3Srb36gZZN{[Xl? MUfEbZNxdGGlZX3lcpQhd2ZiW{PIYY1mdGG2b37pckBnem:vIILheEBOXDJicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVy2JYXzwbIEyPiClZXzsd{whU2l;MD6wNFA2QM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ|N{[0OEc,OjF{M{e2OFQ9N2F-
CHO-Galpha16 NGL4W21HfW6ldHnvckBie3OjeR?= MX\EbZNxdGGlZX3lcpQhd2ZiW{PIYY1mdGG2b37pckBnem:vIILheEBOXDFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVy2JYXzwbIEyPiClZXzsd{whU2l;MD6wNFA6Qc7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ|N{[0OEc,OjF{M{e2OFQ9N2F-
CHO-Galpha16 M4jqeWZ2dmO2aX;uJIF{e2G7 M{PJcVIxKG2rboO= MkL3Rolv\GmwZzDh[oZqdmm2eTD0c{Bz[XRiTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tS4FteGijMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgR4EzMyCvb3LpcIl7[XSrb36gZYZ1\XJiMkCgcYlveyCkeTDGUGlRWiCjc4PhfUwhTUN3ME2wMlAxOTIQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ|N{[0OEc,OjF{M{e2OFQ9N2F-
CHO NHHDdlBHfW6ldHnvckBie3OjeR?= NITOSI5FcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODByMkNOwG0> M4mwdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{myPFU5Lz5{MUO5Nlg2QDxxYU6=
CHO MVrGeY5kfGmxbjDhd5NigQ>? Ml3KSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlAxODN3zszN NF;FWYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO5Nlg2QCd-MkGzPVI5PTh:L3G+
HEK293 NX;vV3NuTnWwY4Tpc44h[XO|YYm= NXnvbm0zTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndMYlw\G:vZXzheI9vcW5iZoLvcUBpfW2jbjDNWFEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNFAzPc7:TR?= M12zPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEKwPFYyLz5{MUSyNFg3OTxxYU6=
HEK293 MnfxSpVv[3Srb36gZZN{[Xl? NUXsPIw6TGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndMYlw\G:vZXzheI9vcW5iZoLvcUBpfW2jbjDNWFIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNFA{PM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR{MEi2NUc,OjF2MkC4OlE9N2F-
CHO M3;nXmZ2dmO2aX;uJIF{e2G7 NGfTV4tC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJG1VOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KFt|NWPdS3RR\2GvbXGgZolv\GmwZzDhd5NigSxiRVO1NF0xNjByMESy{txO NUT5Wo1ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0NlA5PjFpPkKxOFIxQDZzPD;hQi=>
CHO MXnGeY5kfGmxbjDhd5NigQ>? MWXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHL5JHs{PVOfR2TQ[4FudWFiYnnu[Ilv\yCjc4PhfUwhTUN3ME2wMlAxOTYQvF2= NUC5elF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0NlA5PjFpPkKxOFIxQDZzPD;hQi=>
CHO NHX3dm5HfW6ldHnvckBie3OjeR?= M1KzXlMxKG2rboO= MXPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiZn;yd4twdGmwLXnu[JVk\WRiY1HNVEBi[2O3bYXsZZRqd25iYX\0[ZIhOzBibXnud{BjgSCHbm\pd4lwdiCPdXz0bYxi[mWuIGDsZZRmKFKnYXTldpMtKEWFNUC9NE4xODByMkW3{txO MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR5M{[yOUc,OjF2N{O2NlU9N2F-
CHO MYPGeY5kfGmxbjDhd5NigQ>? MVrEbZNxdGGlZX3lcpQhd2ZiW{GyOWleNTJvaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgc44hS0iRIHPlcIx{KG2rY4Lvd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3m9NE4xODB{Md88US=> NVm1doNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0O|M3OjVpPkKxOFc{PjJ3PD;hQi=>
CHO M2D2VmZ2dmO2aX;uJIF{e2G7 NH7QTXhFcXOybHHj[Y1mdnRib3[gX|EzPUmfLUKtbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhd25iQ1jPJINmdGy|IHL5JI1q[3Kxc3PpcpRqdGyjdHnvckBkd3WwdHnu[{whU2l;MD6wNFAzPM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR5M{[yOUc,OjF2N{O2NlU9N2F-
CHO NVrsSYg{TnWwY4Tpc44h[XO|YYm= MmnuN|AhdWmwcx?= Moq5RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDNWFIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIH\vdpNsd2yrbj3pcoR2[2WmIHPBUXAh[WOldX31cIF1cW:wIHHmeIVzKDNyIH3pcpMh[nliRX7WbZNqd25iTYXseIlt[WKnbDDQcIF1\SCUZXHk[ZJ{NCCHQ{WwQVAvODByOEWxNe69VQ>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR5M{[yOUc,OjF2N{O2NlU9N2F-
CHO NFjEOGVHfW6ldHnvckBie3OjeR?= NXPoUXlGOzBibXnudy=> M1u1SGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUXQyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iZn;yd4twdGmwLYP0bY12dGG2ZXSgZ2FOWCC{ZXzlZZNmKGGodHXyJFMxKG2rboOgZpkhdXWudHnsZYJmdCCybHH0[UBz\WGmZYKsJGVEPTB;MD6wNFAxOjcQvF2= NX3Rc|ZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1OlgzQTFpPkKxOVY5OjlzPD;hQi=>
CHO MnvmSpVv[3Srb36gZZN{[Xl? NHK2XIpFcXOybHHj[Y1mdnRib3[gNk1cOTJ3SW3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODByMkJOwG0> NG\WeYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW2PFI6OSd-MkG1OlgzQTF:L3G+
CHO M1HTfWZ2dmO2aX;uJIF{e2G7 NV31OZY4TGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3UNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDLbV0xNjByMEK0{txO NYTU[lBlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1OlgzQTFpPkKxOVY5OjlzPD;hQi=>
CHO M3XwXGZ2dmO2aX;uJIF{e2G7 M4DvTFMxKG2rboO= MXPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKG[xcoPrc4xqdi2|dHnteYxifGWmIHPBUXAhemWuZXHz[UBi\nSncjCzNEBucW6|IHL5JI12dHSrbHHi[YwheGyjdHWgdoVi\GW{LDDFR|UxRTBwMECwPFLPxE1? NW\WfoE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1OlgzQTFpPkKxOVY5OjlzPD;hQi=>
HEK293 MmXjSpVv[3Srb36gZZN{[Xl? MWLEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hVVRzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBpfW2jbjDISWszQTNiY3XscJMh[nlicnHkbY9tcWejbnSgZolv\GmwZzDhd5NigSxiS3m9NE4xODB{Nd88US=> MmTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5NkSxPFUoRjJzN{[0NVg2RC:jPh?=
HEK293 M{HOVGZ2dmO2aX;uJIF{e2G7 M4L6emRqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiCPVEKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKGi3bXHuJGhGUzJ7MzDj[YxteyCkeTDyZYRqd2yrZ3Hu[EBjcW6maX7nJIF{e2G7LDDLbV0xNjByMEO0{txO M{PvfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{[0NVg2Lz5{MUe2OFE5PTxxYU6=
NIH3T3 NH7veWdHfW6ldHnvckBie3OjeR?= MUm5NEBucW6| M3T6S2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKHKjdDDOTWg{XDNiY3XscJMh[W[2ZYKgPVAhdWmwczygT4k:OC5yMECyOlPPxE1? MlWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNEe1OVYoRjJ{MES3OVU3RC:jPh?=
NIH3T3 NGTTSlRHfW6ldHnvckBie3OjeR?= MoDYPVAhdWmwcx?= MofaSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hemG2IF7JTFNVOyClZXzsd{Bi\nSncjC5NEBucW6|LDDLbV0xNjByMEOzPFjPxE1? M4jSblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MES3OVU3Lz5{MkC0O|U2PjxxYU6=
CHO NYDr[mtMTnWwY4Tpc44h[XO|YYm= MnrSOlAhdWmwcx?= M3rFdWRqe3CuYXPlcYVvfCCxZjDbN2hedWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYX\0[ZIhPjBibXnud{BjgSCvaXPyc4JmfGFic3PpcpRqdGyjdHnvckBu\XSqb3SsJGtqRTBwMECwNlk3|ryP MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OEiwPEc,OjN{Mki4NFg9N2F-
CHO M33MXmZ2dmO2aX;uJIF{e2G7 MmTGOlAhdWmwcx?= MoXUSIl{eGyjY3Xt[Y51KG:oIGuzTH1u\WyjdH;ubY4h\nKxbTDoeY1idiCPVEKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyCjZoTldkA3OCCvaX7zJIJ6KG2rY4LvZoV1[SC|Y3nueIltdGG2aX;uJI1mfGixZDygT4k:OC5yMEC0NlnPxE1? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OEiwPEc,OjN{Mki4NFg9N2F-
HEK293 Ml;rSpVv[3Srb36gZZN{[Xl? M4SwXlIhcHK| NEH6bHJFcXOybHHj[Y1mdnRib3[gX|EzPUmfMj3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5icnXjc41jcW6jboSgUXQyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNkBpenNiYomg[4FudWFiY3;1cpRqdmduIFvpQVAvODByMt88US=> MlizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NkW4PFUoRjJ|Mk[1PFg2RC:jPh?=
HEK293 NIXlVGdHfW6ldHnvckBie3OjeR?= MX:yJIhzew>? Mmr1SIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTJvaX;kc41mdGG2b37pckBnem:vIHj1cYFvKHKnY3;tZolv[W62IF3UNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEKgbJJ{KGK7IHfhcY1iKGOxdX70bY5oNCCNaU2wMlAxODYQvF2= NFL2eZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K2OVg5PSd-MkOyOlU5QDV:L3G+
CHOK1 NVPHN4o1TnWwY4Tpc44h[XO|YYm= NFnKUlc{KGi{cx?= MVzEbZNxdGGlZX3lcpQhd2ZiW{GyOWleOi2rb3TvcYVt[XSxbnnuJIZzd21iaIXtZY4hemWlb33ibY5idnRibXXsZZRwdmmwIILlZ4VxfG:{IEGg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|IHHmeIVzKDNiaILzMEBMcT1yLkCwNFEy|ryP NFrXSJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwN|A5Oid-MkO0NFMxQDJ:L3G+
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CHO MUXGeY5kfGmxbjDhd5NigQ>? M1HsWlEhcHJ? MoCzSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPTGfLXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiCPVEGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyCrbnP1ZoF1\WRiZn;yJFEhcHJiYomg[4FudWFiY3;1cpRqdmdibXX0bI9lNCCNaU2wMlAxODR4zszN M2r3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzN{K3OFczLz5|MUeyO|Q4OjxxYU6=
NIH3T3 MlvhSpVv[3Srb36gZZN{[Xl? M1fCe2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjVzXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCQSVizWFMh[2WubIOgcYVu[nKjbnXzJIJ6KHKjZHnvcIlo[W6mIHLpcoRqdmdiYYPzZZktKEurPUCuNFAxPjcQvF2= NV7X[JhiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3Nlc1PzJpPkOxO|I4PDd{PD;hQi=>
COS7 MXHGeY5kfGmxbjDhd5NigQ>? NH3hOHgyNjViaILz M1LpfmRqe3CuYXPlcYVvfCCxZjCyMXsyOjVzXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHnuZ5Vj[XSnZDDmc5IhOS53IHjyd{BjgSCpYX3tZUBkd3WwdHnu[{Bu\XSqb3SsJGtqRTBwMECwPFjPxE1? NIXXc4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUeyO|Q4Oid-M{G3Nlc1PzJ:L3G+
CHO Mk\HSpVv[3Srb36gZZN{[Xl? M{fpT|EhcHJ? M1LxS2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjVzXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiaX7jeYJifGWmIH\vdkAyKGi{IHL5JIdidW2jIHPveY51cW6pIH3leIhw\CxiS3m9NE4xODB7Nd88US=> NWPNR3JWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3Nlc1PzJpPkOxO|I4PDd{PD;hQi=>
CHO NGjBb|dHfW6ldHnvckBie3OjeR?= MnfiRolv\GmwZzDh[oZqdmm2eTDh[4FqdnO2IHj1cYFvKE2nbHH0c45qdiC{ZXPldJRweiC2eYDlJFFCKGK7IHTpd5Bt[WOnbXXueEBw\iCdMUK1TX1qd2SxbXXsZZRwdmmwIIP0ZYJtgSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBMcT1yLkCwNFE{|ryP NVKwVlNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDVxJ{7DbGVOSkx:L3G+
CHO NWjiXWxvTnWwY4Tpc44h[XO|YYm= NVjC[ppOTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndbY9ld22nbHH0c45qdiCocn;tJIh2dWGwIF3lcIF1d26rbjDy[YNmeHSxcjD0fZBmKDGDIHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUCuNFAxOs7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OU8oRkOqRV3CUFww[T5?
CHO Mon0SpVv[3Srb36gZZN{[Xl? NX3IWnhCSmmwZHnu[{Bi\m[rbnn0fUBi\2GrboP0JIh2dWGwIF3lcIF1d26rbjDy[YNmeHSxcjD0fZBmKDGEIHL5JIRqe3CuYXPlcYVvfCCxZjDbNVI2UV2rb3TvcYVt[XSxbnnuJJN1[WKueTDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlAxODJ|zszN NETP[YI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PS9pPlPoSW1DVDxxYU6=
CHO MWjGeY5kfGmxbjDhd5NigQ>? M{jqVmlvcGmkaYTpc44hd2ZiMj3bNVI2UV2rb3TvcYVt[XSxbnnuJIJqdmSrbnegeI8hcHWvYX6gUYVt[XSxbnnuJJJm[2WydH;yJJR6eGViMVKg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xODB{Od88US=> MnjWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFUwLz6FaFXNRmw9N2F-
CHO MYXGeY5kfGmxbjDhd5NigQ>? NXPJPXNTTGm|cHzhZ4Vu\W62IH;mJFIuYzF{NVndMYlw\G:vZXzheI9vcW5iZoLvcUBpfW2jbjDNWFEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczygT4k:OC5yMEC0OVcy|ryP MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OU8oRkOqRV3CUFww[T5?
CHO M4XnbGZ2dmO2aX;uJIF{e2G7 M3;qc2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYomgS4FudWFiY3;1cpRqdmduIFvpQVAvODByNEW3Ne69VQ>? M2TabFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ2Nyd-Q3jFUWJNRC:jPh?=
CHO M4rFTWZ2dmO2aX;uJIF{e2G7 NYXCSXlSOSCqch?= MoXxSIl{eGyjY3Xt[Y51KG:oIEKtX|EzPUmfLXnv[I9u\WyjdH;ubY4h\nKxbTDoeY1idiCvZXzheI9vcW5icnXj[ZB1d3JiTWSxJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYX\0[ZIhOSCqcjDifUBo[W2vYTDjc5VvfGmwZzDhcoFtgXOrczygT4k:OC5yMEC0OVcy|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OU8oRkOqRV3CUFww[T5?
CHO MWrGeY5kfGmxbjDhd5NigQ>? M1;5b2Rqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5iTWSyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODByOUW1{txO NXfFTXU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDVxJ{7DbGVOSkx:L3G+
CHO NWPLenVtTnWwY4Tpc44h[XO|YYm= MUTEbZNxdGGlZX3lcpQhd2ZiMj3bNVI2UV1vaX;kc41mdGG2b37pckBnem:vIHj1cYFvKE2WMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGK7IFfhcY1iKGOxdX70bY5oNCCNaU2wMlAxODl3Nd88US=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OU8oRkOqRV3CUFww[T5?
CHO M2G0dGZ2dmO2aX;uJIF{e2G7 MXGxJIhz M3v6dWRqe3CuYXPlcYVvfCCxZjCyMXsyOjWLXT3pc4RwdWWuYYTvcolvKG[{b32gbJVu[W5ibXXsZZRwdmmwIILlZ4VxfG:{IF3UNkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHmeIVzKDFiaIKgZpkh\2GvbXGgZ492dnSrbnegZY5idHm|aYOsJGtqRTBwMECwPVU2|ryP NYrrS3NIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDVxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MT1 / MT2 / p-CREB / CREB / BDNF; 

PubMed: 29570621     


Western blot analysis of MT1, MT2, p-CREB, CREB, and BDNF in two cell lines. HT-22 and H19-7 cells were treated with 1 µM melatonin for 2 h;

p-Raf / p-ERK / p-p90RSK / PKAα / p-AKT / p-JNK / p-CaMKII ; 

PubMed: 29570621     


Melatonin regulated the expression of kinases associated with long-term memory processing. HT-22 cells were treated with 1 µM melatonin for 2 h. The expressions of MT1, p-CREB, CREB, p-Raf, Raf, p-ERK, p-p90RSK, BDNF, PKAα, p-AKT, p-JNK, and p-CaMKII were confirmed by Western blot analysis

29570621

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 47 mg/mL (202.34 mM)
Water Insoluble
Ethanol ''47 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 232.28
化学式

C13H16N2O2

CAS号 73-31-4
储存条件 粉状
溶于溶剂
别名 NSC 56423, N-Acetyl-5-methoxytryptamine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04882280 Not yet recruiting Behavioral: Light Sleep Palo Alto Veterans Institute for Research|VA Palo Alto Health Care System July 2022 Not Applicable
NCT04521972 Not yet recruiting Device: Room light/light bulb Pregnancy Related|Labor; Poor|Uterine Contractions Weak Michigan State University|McLaren Health Care October 1 2021 Not Applicable

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

MT Receptor Signaling Pathway Map

相关MT Receptor产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID