Home Cytoskeletal Signaling Antineoplastic and Immunosuppressive Antibiotics inhibitor - Autophagy modulator - Microtubule Associated inhibitor - Apoptosis related activator Vincristine sulfate 仅限科研使用

Vincristine sulfate

别名: Leurocristine sulfate, NSC-67574 sulfate, 22-Oxovincaleukoblastine sulfate

Vincristine sulfate通过与微管蛋白结合而抑制微管聚合，IC50为32 μM。Vincristine sulfate可诱导凋亡。

Vincristine sulfate Chemical Structure

Vincristine sulfate Chemical Structure

CAS: 2068-78-2

规格 价格 库存 购买数量
10mM (1mL in DMSO) 734.41 现货
10mg 582.05 现货
50mg 2229.59 现货
200mg 7118.45 现货
1g 24324.3 现货
更大包装 有超大折扣

400-668-6834

info@selleck.cn
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Vincristine sulfate相关产品

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息(PMID)
H1299/ICAM-3 Cell Viability Assay 0-20 nM 96 h inhibits cell viability dose dependently 24177012
NCI-H1299/pcDNA3 Cell Viability Assay 0-20 nM 96 h inhibits cell viability dose dependently 24177012
Ramos Apoptosis Assay 2 μM 48 h induces 34.6%±1.92% apoptosis 24256491
CCD18Co Growth Inhibition Assay 0-1000 nM 48 h inhibits cell growth dose dependently 24403453
DLD-1 Growth Inhibition Assay 0-1000 nM 48 h inhibits cell growth dose dependently 24403453
CCD18Co Function Assay 100 nM 48 h promotes RUNX3 demethylation  24403453
DLD-1 Function Assay 100 nM 48 h promotes RUNX3 demethylation  24403453
U-251MG Growth Inhibition Assay 0.1 μM 24/72 h inhibits cell growth time dependently 24530235
A-172  Growth Inhibition Assay 0.1 μM 24/72 h inhibits cell growth time dependently 24530235
SUDHL6  Function Assay 1 μM 72 h induces significant apoptotic morphology changes cotrested with BCL11A siRNA 24961604
SUDHL6  Growth Inhibition Assay 1 μM 48/72 h inhibits cell viability significantly 24961604
P12 Growth Inhibition Assay 1-3 nM 48 h decreases viable cell numbers dose dependently 25156146
CEM Growth Inhibition Assay 1-3 nM 48 h decreases viable cell numbers dose dependently 25156146
Jurkat Growth Inhibition Assay 1-3 nM 48 h decreases viable cell numbers dose dependently 25156146
SK-MEL-28  Function Assay 30 nM 6 h induces G2–M cell-cycle arrest 25313010
Raji Function Assay 0.5 μM 72 h abolishes autophagy  25446377
HepG2-HBV1.1 Growth Inhibition Assay 0.1 μM 12-72 h induces cell cycle arrest at S-phase 25663769
HepG2-HBV1.1 Growth Inhibition Assay 0.1 μM 12-72 h induces cell cycle arrest at S-phase 25663769
HepG2-HBV1.1 Growth Inhibition Assay 0.1 μM 1-5 d inhibits the proliferation time dependently 25663769
HepG2-HBV1.1 Growth Inhibition Assay 0.1 μM 1-5 d inhibits the proliferation time dependently 25663769
HepG2-HBV1.1 Function Assay 0.1 μM 48 h promotes cell excretion of hepatitis B virus nucleocapsids instead of hepatitis B virus Dane particles 25663769
HepG2-HBV1.1 Function Assay 0.1 μM 24 h promotes hepatitis B virus protein expression 25663769
HepG2.2.15 Function Assay 0.1 μM 24 h promotes hepatitis B virus protein expression 25663769
HepG2-HBV1.1 Apoptosis Assay 0-0.5 μM 24 h induces apoptosis dose dependently 25663769
HepG2.2.15 Cell Viability Assay 0-0.5 μM 24 h decreases cell viability dose dependently 25663769
NCI-H1299/pcDNA3 Function Assay 1/5/10/20 nM 96 h induces cleavage of caspases-3, 8, 9, and PARP 24177012
H1299/ICAM-3 Function Assay 1/5/10/20 nM 96 h induces cleavage of caspases-3, 8, 9, and PARP 24177012
K562 Apoptosis Assay 0.3 μM 24 h induces apoptosis significantly 24135937
K562/ADR Apoptosis Assay 3 μM 24 h induces apoptosis 24135937
K562 Cell Viability Assay 3.75–60 nM 72 h  inhibits cell viability dose dependently 23877223
lucena Cell Viability Assay 3.75–60 nM 72 h  no effect 23877223
FEPS Cell Viability Assay 3.75–60 nM 72 h  no effect 23877223
Jurkat Apoptosis Assay 5 μg/ml  24 h induces apoptosis significantly 23810409
Jurkat Growth Inhibition Assay 5 μg/ml  24 h arrest Jurkat cells in G2/M phase 23810409
SGC-7901 Growth Inhibition Assay 0-10 μg/ml 24/48/72 h inhibits cell growth in dose and time dependent manner 23743572
SGC-7901/VCR Growth Inhibition Assay 0-10 μg/ml 24/48/72 h inhibits cell growth in dose and time dependent manner 23743572
JFCR39  Growth Inhibition Assay 1 µM 24 h markedly increases the number of G2/M phase cells 23598276
A549 Function Assay 100 nM 16 h leads to a loss of microtubules 23598276
SH-SY5Y  Growth Inhibition Assay 0.001-10 μM 24 h IC50=0.113±0.012 μM 23129065
SH-SY5Y  Growth Inhibition Assay 0.001-10 μM 48 h IC50=0.078±0.009 μM 23129065
SH-SY5Y  Growth Inhibition Assay 0.001-10 μM 72 h IC50=0.051±0.008 μM 23129065
SH-SY5Y Apoptosis Assay 0.1 μM 0-24 h induces apoptosis of SH-SY5Y cells following cell cycle arrest at the G2/M phase 23129065
SH-SY5Y Apoptosis Assay 0.1 μM 0-24 h induces mitotic arrest  23129065
A549/EGFP  Growth Inhibition Assay 0.01-1000 μM IC50=86.7 ± 29.1 μM 23634282
A549/Slug Growth Inhibition Assay 0.01-1000 μM IC50=9.7 ± 3.1 μM 23634282
HepG2 Growth Inhibition Assay 24 h IC50=52.5 μM 24341688
TCC Growth Inhibition Assay 48 h IC50=50 nM 24716944
TCC Growth Inhibition Assay 24 h IC50=70 nM 24716944
LNCAP Cytotoxicity assay 72 hrs Cytotoxicity against human LNCAP cells after 72 hrs by alamar blue assay, IC50 = 0.0029 μM. 20732809
LNCAP Cytotoxicity assay 72 hrs Cytotoxicity against human LNCAP cells after 72 hrs by alamar blue assay, IC50 = 0.0061 μM. 23215348
PC3 Cytotoxicity assay 72 hrs Cytotoxicity against human PC3 cells after 72 hrs by alamar blue assay, IC50 = 0.011 μM. 20732809
PC3 Cytotoxicity assay 72 hrs Cytotoxicity against human PC3 cells after 72 hrs by alamar blue assay, IC50 = 0.015 μM. 23215348
PC3 Cytotoxicity assay 72 hrs Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 72 hrs by alamar blue assay, IC50 = 0.0153 μM. 23489291
NFF Cytotoxicity assay 72 hrs Cytotoxicity against human NFF cells assessed as inhibition of cell viability after 72 hrs by alamar blue assay, IC50 = 0.0169 μM. 23489291
NFF Cytotoxicity assay 72 hrs Cytotoxicity against human NFF cells after 72 hrs by alamar blue assay, IC50 = 0.017 μM. 23215348
A549 Cytotoxicity assay 72 hrs Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay, IC50 = 0.02 μM. 23215348
KB Cytotoxicity assay 72 hrs Cytotoxicity against human KB cells after 72 hrs by SRB assay, IC50 = 0.02 μM. 26522953
PC3 Antiproliferative assay 48 hrs Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay, IC50 = 0.0276 μM. 28038323
HEK Cytotoxicity assay 72 hrs Cytotoxicity against human HEK cells after 72 hrs by alamar blue assay, IC50 = 0.031 μM. 23215348
HeLa Cytotoxicity assay 72 hrs Cytotoxicity against human HeLa cells assessed as inhibition of cell viability after 72 hrs by alamar blue assay, IC50 = 0.0358 μM. 23489291
HeLa Cytotoxicity assay 72 hrs Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay, IC50 = 0.036 μM. 23215348
BT549 Antiproliferative assay 48 hrs Antiproliferative activity against human BT549 cells after 48 hrs by MTT assay, IC50 = 0.05472 μM. 28038323
DU145 Antiproliferative assay 48 hrs Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay, IC50 = 0.0723 μM. 28038323
Leukemia cells Antitumor assay 48 hrs Antitumor activity against human Leukemia cells after 48 hrs by SRB assay, GI50 = 0.1 μM. 19006285
Leukemia cells Antitumor assay 48 hrs Antitumor activity against human Leukemia cells after 48 hrs by SRB assay, GI = 0.1 μM. 19006285
Colon cancer cells Antitumor assay 48 hrs Antitumor activity against human Colon cancer cells after 48 hrs by SRB assay, GI50 = 0.1 μM. 19006285
Colon cancer cells Antitumor assay 48 hrs Antitumor activity against human Colon cancer cells after 48 hrs by SRB assay, GI = 0.1 μM. 19006285
Leukemia cells Growth inhibition assay 48 hrs Growth inhibition of human leukemia cells after 48 hrs, GI50 = 0.1 μM. 20684599
Colon cancer cells Growth inhibition assay 48 hrs Growth inhibition of human colon cancer cells after 48 hrs, GI50 = 0.1 μM. 20684599
Leukemia cells Cytotoxicity assay 48 hrs Cytotoxicity against human leukemia cells after 48 hrs, GI50 = 0.1 μM. 24747749
Colon cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human colon cancer cells after 48 hrs, GI50 = 0.1 μM. 24747749
Leukemia cells Cytotoxicity assay 48 hrs Cytotoxicity against human leukemia cells after 48 hrs, GI50 = 0.1 μM. 22283430
Colon cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human COLON cells after 48 hrs, GI50 = 0.1 μM. 22283430
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 0.1166 μM. 28038323
CNS Antitumor assay 48 hrs Antitumor activity against human CNS cancer cells after 48 hrs by SRB assay, GI50 = 0.12589 μM. 19006285
prostate cancer cells Antitumor assay 48 hrs Antitumor activity against human prostate cancer cells after 48 hrs by SRB assay, GI50 = 0.12589 μM. 19006285
CNS Antitumor assay 48 hrs Antitumor activity against human CNS cancer cells after 48 hrs by SRB assay, GI = 0.13 μM. 19006285
prostate cancer cells Antitumor assay 48 hrs Antitumor activity against human prostate cancer cells after 48 hrs by SRB assay, GI = 0.13 μM. 19006285
prostate cancer cells Growth inhibition assay 48 hrs Growth inhibition of human prostate cancer cells after 48 hrs, GI50 = 0.13 μM. 20684599
CNS Growth inhibition assay 48 hrs Growth inhibition of human CNS cancer cells after 48 hrs, GI50 = 0.13 μM. 20684599
CNS Cytotoxicity assay 48 hrs Cytotoxicity against human CNS cancer cells after 48 hrs, GI50 = 0.13 μM. 24747749
prostate cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human prostate cancer cells after 48 hrs, GI50 = 0.13 μM. 24747749
prostate cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human prostate cancer cells after 48 hrs, GI50 = 0.13 μM. 22283430
CNS Cytotoxicity assay 48 hrs Cytotoxicity against human CNS cancer cells after 48 hrs, GI50 = 0.13 μM. 22283430
Melanoma cells Antitumor assay 48 hrs Antitumor activity against human Melanoma cells after 48 hrs by SRB assay, GI50 = 0.15849 μM. 19006285
Melanoma cells Antitumor assay 48 hrs Antitumor activity against human Melanoma cells after 48 hrs by SRB assay, GI = 0.16 μM. 19006285
Melanoma cells Cytotoxicity assay 48 hrs Cytotoxicity against human melanoma cells after 48 hrs, GI50 = 0.16 μM. 24747749
Melanoma cells Cytotoxicity assay 48 hrs Cytotoxicity against human melanoma cells after 48 hrs, GI50 = 0.16 μM. 22283430
A549 Antiproliferative assay 48 hrs Antiproliferative activity against human A549 cells after 48 hrs by MTT assay, IC50 = 0.1715 μM. 28038323
NSCLC Antitumor assay 48 hrs Antitumor activity against human NSCLC cells after 48 hrs by SRB assay, GI = 0.25 μM. 19006285
NSCLC Growth inhibition assay 48 hrs Growth inhibition of human NSCLC cells after 48 hrs, GI50 = 0.25 μM. 20684599
NSCLC Cytotoxicity assay 48 hrs Cytotoxicity against human NSCLC cells after 48 hrs, GI50 = 0.25 μM. 22283430
NSCLC Cytotoxicity assay 48 hrs Cytotoxicity against human NSCLC cells after 48 hrs, GI50 = 0.25 μM. 24747749
NSCLC Antitumor assay 48 hrs Antitumor activity against human NSCLC cells after 48 hrs by SRB assay, GI50 = 0.25119 μM. 19006285
ovarian cancer cells Antitumor assay 48 hrs Antitumor activity against human ovarian cancer cells after 48 hrs by SRB assay, GI50 = 0.31623 μM. 19006285
renal cancer cells Antitumor assay 48 hrs Antitumor activity against human renal cancer cells after 48 hrs by SRB assay, GI50 = 0.31623 μM. 19006285
breast cancer cells Antitumor assay 48 hrs Antitumor activity against human Breast cancer cells after 48 hrs by SRB assay, GI50 = 0.31623 μM. 19006285
ovarian cancer cells Antitumor assay 48 hrs Antitumor activity against human ovarian cancer cells after 48 hrs by SRB assay, GI = 0.32 μM. 19006285
renal cancer cells Antitumor assay 48 hrs Antitumor activity against human renal cancer cells after 48 hrs by SRB assay, GI = 0.32 μM. 19006285
breast cancer cells Antitumor assay 48 hrs Antitumor activity against human Breast cancer cells after 48 hrs by SRB assay, GI = 0.32 μM. 19006285
renal cancer cells Growth inhibition assay 48 hrs Growth inhibition of human renal cancer cells after 48 hrs, GI50 = 0.32 μM. 20684599
breast cancer cells Growth inhibition assay 48 hrs Growth inhibition of human breast cancer cells after 48 hrs, GI50 = 0.32 μM. 20684599
ovarian cancer cells Growth inhibition assay 48 hrs Growth inhibition of human Ovarian cancer cells after 48 hrs, GI50 = 0.32 μM. 20684599
breast cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human breast cancer cells after 48 hrs, GI50 = 0.32 μM. 24747749
renal cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human renal cancer cells after 48 hrs, GI50 = 0.32 μM. 24747749
ovarian cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human ovarian cancer cells after 48 hrs, GI50 = 0.32 μM. 24747749
renal cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human renal cancer cells after 48 hrs, GI50 = 0.32 μM. 22283430
ovarian cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human ovarian cancer cells after 48 hrs, GI50 = 0.32 μM. 22283430
breast cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human breast cancer cells after 48 hrs, GI50 = 0.32 μM. 22283430
Lu1 Cytotoxicity assay 72 hrs Cytotoxicity against human Lu1 cells after 72 hrs by SRB assay, IC50 = 0.48 μM. 26522953
MCF7 Cytotoxicity assay 72 hrs Cytotoxicity against human MCF7 cells after 72 hrs by SRB assay, IC50 = 0.51 μM. 26522953
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by SRB assay, IC50 = 0.69 μM. 26522953
COLON Growth inhibition assay 48 hrs Growth inhibition of human colon cancer cells after 48 hrs, TGI = 3.98 μM. 20684599
COLON Cytotoxicity assay 48 hrs Cytotoxicity against human colon cancer cells after 48 hrs, TGI = 3.98 μM. 24747749
COLON Cytotoxicity assay 48 hrs Cytotoxicity against human COLON cells after 48 hrs, TGI = 3.98 μM. 22283430
COLON Antitumor assay 48 hrs Antitumor activity against human Colon cancer cells after 48 hrs by SRB assay, TGI = 3.98107 μM. 19006285
CNS Antitumor assay 48 hrs Antitumor activity against human CNS cancer cells after 48 hrs by SRB assay, TGI = 6.30957 μM. 19006285
prostate cancer cells Antitumor assay 48 hrs Antitumor activity against human prostate cancer cells after 48 hrs by SRB assay, TGI = 6.30957 μM. 19006285
prostate cancer cells Growth inhibition assay 48 hrs Growth inhibition of human prostate cancer cells after 48 hrs, TGI = 6.31 μM. 20684599
CNS Growth inhibition assay 48 hrs Growth inhibition of human CNS cancer cells after 48 hrs, TGI = 6.31 μM. 20684599
CNS Cytotoxicity assay 48 hrs Cytotoxicity against human CNS cancer cells after 48 hrs, TGI = 6.31 μM. 24747749
prostate cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human prostate cancer cells after 48 hrs, TGI = 6.31 μM. 24747749
prostate cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human prostate cancer cells after 48 hrs, TGI = 6.31 μM. 22283430
CNS Cytotoxicity assay 48 hrs Cytotoxicity against human CNS cancer cells after 48 hrs, TGI = 6.31 μM. 22283430
Melanoma cells Growth inhibition assay 48 hrs Growth inhibition of human melanoma cells after 48 hrs, TGI = 7.94 μM. 20684599
breast cancer cells Growth inhibition assay 48 hrs Growth inhibition of human breast cancer cells after 48 hrs, TGI = 7.94 μM. 20684599
breast cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human breast cancer cells after 48 hrs, TGI = 7.94 μM. 24747749
Melanoma cells Cytotoxicity assay 48 hrs Cytotoxicity against human melanoma cells after 48 hrs, TGI = 7.94 μM. 24747749
Melanoma cells Cytotoxicity assay 48 hrs Cytotoxicity against human melanoma cells after 48 hrs, TGI = 7.94 μM. 22283430
breast cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human breast cancer cells after 48 hrs, TGI = 7.94 μM. 22283430
Melanoma cells Antitumor assay 48 hrs Antitumor activity against human Melanoma cells after 48 hrs by SRB assay, TGI = 7.94328 μM. 19006285
breast cancer cells Antitumor assay 48 hrs Antitumor activity against human Breast cancer cells after 48 hrs by SRB assay, TGI = 7.94328 μM. 19006285
Leukemia cells Antitumor assay 48 hrs Antitumor activity against human Leukemia cells after 48 hrs by SRB assay, TGI = 15.8489 μM. 19006285
NSCLC Antitumor assay 48 hrs Antitumor activity against human NSCLC cells after 48 hrs by SRB assay, TGI = 15.8489 μM. 19006285
NSCLC Growth inhibition assay 48 hrs Growth inhibition of human NSCLC cells after 48 hrs, TGI = 15.85 μM. 20684599
Leukemia cells Growth inhibition assay 48 hrs Growth inhibition of human leukemia cells after 48 hrs, TGI = 15.85 μM. 20684599
NSCLC Cytotoxicity assay 48 hrs Cytotoxicity against human NSCLC cells after 48 hrs, TGI = 15.85 μM. 22283430
Leukemia cells Cytotoxicity assay 48 hrs Cytotoxicity against human leukemia cells after 48 hrs, TGI = 15.85 μM. 24747749
NSCLC Cytotoxicity assay 48 hrs Cytotoxicity against human NSCLC cells after 48 hrs, TGI = 15.85 μM. 24747749
Leukemia cells Cytotoxicity assay 48 hrs Cytotoxicity against human leukemia cells after 48 hrs, TGI = 15.85 μM. 22283430
renal cancer cells Growth inhibition assay 48 hrs Growth inhibition of human renal cancer cells after 48 hrs, TGI = 19.95 μM. 20684599
ovarian cancer cells Growth inhibition assay 48 hrs Growth inhibition of human Ovarian cancer cells after 48 hrs, TGI = 19.95 μM. 20684599
renal cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human renal cancer cells after 48 hrs, TGI = 19.95 μM. 24747749
ovarian cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human ovarian cancer cells after 48 hrs, TGI = 19.95 μM. 24747749
renal cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human renal cancer cells after 48 hrs, TGI = 19.95 μM. 22283430
ovarian cancer cells Cytotoxicity assay 48 hrs Cytotoxicity against human ovarian cancer cells after 48 hrs, TGI = 19.95 μM. 22283430
ovarian cancer cells Antitumor assay 48 hrs Antitumor activity against human ovarian cancer cells after 48 hrs by SRB assay, TGI = 19.9526 μM. 19006285
renal cancer cells Antitumor assay 48 hrs Antitumor activity against human renal cancer cells after 48 hrs by SRB assay, TGI = 19.9526 μM. 19006285
PC3 Cytotoxicity assay 48 hrs Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay, IC50 = 28.11 μM. 29174816
HEK293/MRP1 Growth Inhibition Assay IC50=0.055±0.0120 μM 24284783
HEK293/pcDNA3.1 Growth Inhibition Assay IC50=0.004±0.0003 μM 24284783
MCF-7 Growth Inhibition Assay IC50=0.0159±0.0062 μM 24704556
MCF-7/ADR Growth Inhibition Assay IC50=4.4826±0.2070 μM 24704556
HepG2 Growth Inhibition Assay IC50=0.0115±0.0017 μM 24704556
HepG2/ADM Growth Inhibition Assay IC50=2.7863±0.2371 μM 24704556
HEK293/ABCC1 Growth Inhibition Assay IC50=19.29 ± 2.08 nM 24726739
HEK293/pcDNA3.1 Growth Inhibition Assay IC50=1.45 ± 0.20 nM 24726739
SW620/AD300 Growth Inhibition Assay IC50=909.60 ± 8.91 nM 24726739
SW620 Growth Inhibition Assay IC50=7.80 ± 0.08 nM 24726739
MCF-7-R Cytotoxicity Assay IC50=5.539 ± 0.144 nM 24858827
A549-R Cytotoxicity Assay IC50=12.178 ± 0.333 nM 24858827
MCF-7-R Growth Inhibition Assay IC50=5.539 ± 0.144 nM 24858827
MCF-7-WT Growth Inhibition Assay IC50=17.752 ± 0.218 nM 24858827
A549-R Growth Inhibition Assay IC50=12.178 ± 0.333 nM 24858827
A549-WT Growth Inhibition Assay IC50=19.437 ± 0.594 nM 24858827
SHSY5Y Growth Inhibition Assay IC50=8.2±0.6 nM 24921920
LAN1 Growth Inhibition Assay IC50=2.3±0.2 nM 24921920
SKNAS Growth Inhibition Assay IC50=1.5±0.2 nM 24921920
IGNR91 Growth Inhibition Assay IC50=24.3±1.7 nM 24921920
SKNBe2C Growth Inhibition Assay IC50=32.8±4.0 nM 24921920
HCT-116 Growth Inhibition Assay GI50=5 nM 24927857
KBv200 (ABCB1) Growth Inhibition Assay IC50=0.3462 ± 0.0066 μM 25058526
KB Growth Inhibition Assay IC50=0.0049 ± 0.0001 μM 25058526
H157 Growth Inhibition Assay IC50=1.03 ± 0.04 miu2M 25257911
W1 Growth Inhibition Assay IC50=0.0032 μM 24140176
W1VR Growth Inhibition Assay IC50=0.056 μM 24140176
K562 Growth Inhibition Assay IC50=0.032 ± 0.001 μM 24135937
K562/ADR Growth Inhibition Assay IC50=3.261 ± 0.412 μM 24135937
A549 Growth Inhibition Assay IC50=0.10 ± 0.03 μM 23971075
A2780 Growth Inhibition Assay IC50=3.5 mM 23829203
ACHN Growth Inhibition Assay IC50<0.1 mM 23829203
U-937 Growth Inhibition Assay IC50<34 nM 23829203
Hep-2 Growth Inhibition Assay IC50=0.04±0.01 μM 23780424
Hep-2/v Growth Inhibition Assay IC50=1.8±0.20 μM 23780424
SGC-7901 Apoptosis Assay induces apoptosis significantly 23743572
SGC-7901/VCR Apoptosis Assay induces apoptosis significantly 23743572
KB-3-1 Growth Inhibition Assay IC50=1.66 ± 0.162 μM 23673445
KB-C2 Growth Inhibition Assay IC50=202.56 ± 42.481 μM 23673445
KB-3-1 Growth Inhibition Assay IC50=1.26 ± 0.027 μM 23673445
KB-V1 Growth Inhibition Assay IC50=208.85 ± 20.4 μM 23673445
HEK293/pcDNA3.1 Growth Inhibition Assay IC50=24.1 ± 0.204 μM 23673445
HEK293/ABCB1 Growth Inhibition Assay IC50=3512.8 ± 378.391 μM 23673445
SGC7901 Growth Inhibition Assay IC50=1.26 ± 0.11 μg/ml 23564482
SGC7901/LV-NC Growth Inhibition Assay IC50=1.77 ± 0.16 μg/ml 23564482
SGC7901/LV-SGO1 Growth Inhibition Assay IC50=4.36 ± 0.37 μg/ml 23564482
SGC7901/VCR Growth Inhibition Assay IC53=20.53 ± 1.96 μg/ml 23564482
SGC7901/VCR-NC Growth Inhibition Assay IC50=19.86 ± 2.01 μg/ml 23564482
SGC7901/VCR-si-SGO1 Growth Inhibition Assay IC50=6.18 ± 1.03 μg/ml 23564482
SGC7901/ADR Growth Inhibition Assay IC50=7.85 ± 0.64 μg/ml 23564482
SGC7901/ADR-NC Growth Inhibition Assay IC50=8.93 ± 0.68 μg/ml 23564482
SGC7901/ADR-si-SGO1 Growth Inhibition Assay IC50=3.46 ± 0.29 μg/ml 23564482
Colon cancer cells Cytotoxicity assay Cytotoxicity against human colon cancer cells, GI50 = 0.1 μM. 18598018
Leukemia cells Cytotoxicity assay Cytotoxicity against human leukemia cells, GI50 = 0.1 μM. 18598018
Leukemia cells Antitumor assay Antitumor activity against leukemia cells, GI50 = 0.1 μM. 17559205
Colon cancer cells Antitumor assay Antitumor activity against colon tumor cells, GI50 = 0.1 μM. 17559205
central nervous system cancer cells Cytotoxicity assay Cytotoxicity against human central nervous system cancer cells, GI50 = 0.12589 μM. 18598018
prostate cancer cells Cytotoxicity assay Cytotoxicity against human prostate cancer cells, GI50 = 0.12589 μM. 18598018
CNS Antitumor assay Antitumor activity against CNS tumor cells, GI50 = 0.12589 μM. 17559205
prostate cancer cells Antitumor assay Antitumor activity against prostate tumor cells, GI50 = 0.12589 μM. 17559205
Melanoma cells Cytotoxicity assay Cytotoxicity against human melanoma cells, GI50 = 0.15849 μM. 18598018
Melanoma cells Antitumor assay Antitumor activity against melanoma cells, GI50 = 0.15849 μM. 17559205
Melanoma cells Growth inhibition assay Growth inhibition of human melanoma cells after 48 hrs, GI50 = 0.16 μM. 20684599
NSCLC Antitumor assay Antitumor activity against NSCLC cells, GI50 = 0.25119 μM. 17559205
Non-small cell lung carcinoma cells Cytotoxicity assay Cytotoxicity against human Non-small cell lung carcinoma cells, GI50 = 0.25119 μM. 18598018
ovarian cancer cells Cytotoxicity assay Cytotoxicity against human ovarian cancer cells, GI50 = 0.31623 μM. 18598018
renal cancer cells Cytotoxicity assay Cytotoxicity against human renal cancer cells, GI50 = 0.31623 μM. 18598018
breast cancer cells Cytotoxicity assay Cytotoxicity against human breast cancer cells, GI50 = 0.31623 μM. 18598018
renal cancer cells Antitumor assay Antitumor activity against renal tumor cells, GI50 = 0.31623 μM. 17559205
ovarian cancer cells Antitumor assay Antitumor activity against ovarian tumor cells, GI50 = 0.31623 μM. 17559205
breast cancer cells Antitumor assay Antitumor activity against breast tumor cells, GI50 = 0.31623 μM. 17559205
COLON Cytotoxicity assay Cytotoxicity against human colon cancer cells, TGI = 3.98107 μM. 18598018
COLON Antitumor assay Antitumor activity against colon tumor cells, TGI = 3.98107 μM. 17559205
central nervous system cancer cells Cytotoxicity assay Cytotoxicity against human central nervous system cancer cells, TGI = 6.30957 μM. 18598018
prostate cancer cells Cytotoxicity assay Cytotoxicity against human prostate cancer cells, TGI = 6.30957 μM. 18598018
CNS Antitumor assay Antitumor activity against CNS tumor cells, TGI = 6.30957 μM. 17559205
prostate cancer cells Antitumor assay Antitumor activity against prostate tumor cells, TGI = 6.30957 μM. 17559205
Melanoma cells Cytotoxicity assay Cytotoxicity against human melanoma cells, TGI = 7.94328 μM. 18598018
breast cancer cells Cytotoxicity assay Cytotoxicity against human breast cancer cells, TGI = 7.94328 μM. 18598018
Melanoma cells Antitumor assay Antitumor activity against melanoma cells, TGI = 7.94328 μM. 17559205
breast tumor cells Antitumor assay Antitumor activity against breast tumor cells, TGI = 7.94328 μM. 17559205
NSCLC Antitumor assay Antitumor activity against NSCLC cells, TGI = 15.8489 μM. 17559205
Non-small cell lung carcinoma cells Cytotoxicity assay Cytotoxicity against human Non-small cell lung carcinoma cells, TGI = 15.8489 μM. 18598018
Leukemia cells Cytotoxicity assay Cytotoxicity against human leukemia cells, TGI = 15.8489 μM. 18598018
Leukemia cells Antitumor assay Antitumor activity against leukemia cells, TGI = 15.8489 μM. 17559205
ovarian cancer cells Cytotoxicity assay Cytotoxicity against human ovarian cancer cells, TGI = 19.9526 μM. 18598018
renal cancer cells Cytotoxicity assay Cytotoxicity against human renal cancer cells, TGI = 19.9526 μM. 18598018
renal tumor cells Antitumor assay Antitumor activity against renal tumor cells, TGI = 19.9526 μM. 17559205
ovarian tumor cells Antitumor assay Antitumor activity against ovarian tumor cells, TGI = 19.9526 μM. 17559205
neural precursor cells Function assay Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay 17417631
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生物活性

产品描述 Vincristine sulfate通过与微管蛋白结合而抑制微管聚合，IC50为32 μM。Vincristine sulfate可诱导凋亡。
靶点
Microtubules
(Cell-free assay)
32 μM
体外研究(In Vitro)
体外研究活性 Vincristine抑制微管蛋白二聚体聚合亚单位,抑制它们的聚合作用，Ki为85 nM。在低浓度时，Vincristine抑制纺锤体，使染色体分离失败，进而导致中期停滞，并抑制有丝分裂。在较高浓度时，Vincristine可能干扰且诱导全部微管解聚。 Vincristine 诱导肿瘤细胞凋亡，且抑制SH-SY5Y细胞增殖，IC50为0.1 µM。Vincristine诱导有丝分裂停滞，促进caspase-3和-9及cyclin B的表达，但降低cyclin D的表达。 Vincristine通过干扰微管功能而诱导神经毒性形成，这会导致轴突运输堵塞，从而导致轴突变性。
细胞实验 细胞系 B16 黑色素瘤细胞
浓度 10 nM
孵育时间 3 天
方法 细胞按5 × 104 cells/mL的浓度接种在含2 mL培养基的35-mm 实验板上，在37°C下 含5% CO2 和95% 空气 的环境下生长24小时。然后使用有或无10 nM药物的新鲜培养基更换原来的培养基，继续增殖3天。使用胰蛋白酶和EDTA将细胞分离后，每天使用库氏计数器完成细胞计数
实验图片 检测方法 检测指标 实验图片 PMID
Western blot MPM2 / Cyclin B1 / Cdc25c / p-Cdc2 / Cdc2 / Aurora B / p-H3 / p-PLK1 26156322
Growth inhibition assay Cell viability 26156322
体内研究(In Vivo)
体内研究活性 Vincristine 按3 mg/kg剂量单独腹腔注射给药携带双侧皮下移植瘤RH12或RH18的小鼠，平均生长延迟120天和52天以上，且再植指数分别为0.06％和5％。 Vincristine 处理小鼠，通过宿主细胞介导的血管效应及直接的微管蛋白介导的的细胞毒性作用而对皮下结肠38肿瘤起作用。Vincristine (5 mg/kg) 降低肿瘤血流量，降低近75%。
动物实验 Animal Models 携带人类横纹肌肉瘤移植瘤Rh12的鼠
Dosages 3 mg/kg
Administration 腹腔注射
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05844670 Recruiting
Pediatric Cancer
Moi University|Princess Maxima Center for Pediatric Oncology|Amsterdam UMC location VUmc
 April 20 2023 Phase 4
NCT04448834 Withdrawn
B-cell Acute Lymphoblastic Leukemia
Dorothy Sipkins MD PhD|Amgen|Acrotech Biopharma Inc.|Duke University
 January 2022 Phase 2
  • [1]https://pubmed.ncbi.nlm.nih.gov/3986806/
  • [2]https://pubmed.ncbi.nlm.nih.gov/10226730/
  • [3]https://pubmed.ncbi.nlm.nih.gov/23129065/
  • [4]https://pubmed.ncbi.nlm.nih.gov/558047/
  • [5]https://pubmed.ncbi.nlm.nih.gov/3190743/
  • [6]https://pubmed.ncbi.nlm.nih.gov/1827725/

化学信息&溶解度

分子量 923.04 分子式

C46H58N4O14S
CAS号 2068-78-2 SDF Download Vincristine sulfate SDF
Smiles CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C=O)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
储存条件(自收到货起)

体外溶解度
批次：

DMSO : 100 mg/mL ( (108.33 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : 100 mg/mL (108.33 mM)

Ethanol : Insoluble

摩尔浓度计算器

体内溶解配方
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

 动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

mg/kg g μL 只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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常见问题及建议解决方法

问题 1:
I want to use it for mouse in vivo, could you please give us your advice about administration method?

回答：
According to the reference cited on our website, we test its solubility in 4% DMSO/saline, and it is a suspension at 5 mg/mL. This compound can also be dissolved in the vehicle 4% DMSO/30% PEG 300/10% Tween 80/saline at 5 mg/ml as a clear solution for injection.

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