Vincristine (NSC-67574) sulfate

For research use only. Not for use in humans.

目录号:S1241 别名: Leurocristine, Oncovin, 22-Oxovincaleukoblastine 中文名称:硫酸长春新碱

Vincristine (NSC-67574) sulfate Chemical Structure

CAS No. 2068-78-2

Vincristine sulfate (NSC-67574, Leurocristine, Oncovin, 22-Oxovincaleukoblastine)通过与微管蛋白结合而抑制微管聚合,IC50为32 μM。Vincristine sulfate可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 734.41 现货
RMB 582.05 现货
RMB 2229.59 现货
RMB 7118.45 现货
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客户使用Selleck生产的Vincristine (NSC-67574) sulfate发表文献97篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Vincristine sulfate (NSC-67574, Leurocristine, Oncovin, 22-Oxovincaleukoblastine)通过与微管蛋白结合而抑制微管聚合,IC50为32 μM。Vincristine sulfate可诱导凋亡。
靶点
Microtubules [1]
(Cell-free assay)
32 μM
体外研究

Vincristine抑制微管蛋白二聚体聚合亚单位,抑制它们的聚合作用,Ki为85 nM。[1]在低浓度时,Vincristine抑制纺锤体,使染色体分离失败,进而导致中期停滞,并抑制有丝分裂。在较高浓度时,Vincristine可能干扰且诱导全部微管解聚。[2] Vincristine 诱导肿瘤细胞凋亡,且抑制SH-SY5Y细胞增殖,IC50为0.1 µM。Vincristine诱导有丝分裂停滞,促进caspase-3和-9及cyclin B的表达,但降低cyclin D的表达。[3] Vincristine通过干扰微管功能而诱导神经毒性形成,这会导致轴突运输堵塞,从而导致轴突变性。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2.2.15 NH3uWXlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MlvsNE0xNjVizszN M3XKRlI1KGh? NXHQeG17\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIRwe2ViZHXw[Y5l\W62bIm= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ4M{e2PUc,OjV4NkO3Olk9N2F-
HepG2-HBV1.1 NEfnTYxCeG:ydH;zbZMhSXO|YYm= MmfjNE0xNjVizszN MUWyOEBp M{XY[Ylv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= NUXPeYdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2OlM4PjlpPkK1OlY{PzZ7PD;hQi=>
HepG2.2.15 NWHNPIdZTnWwY4Tpc44hSXO|YYm= M3nydFAvOSEQvF2= MnvSNlQhcA>? NHfxZYxxem:vb4Tld{Bp\XCjdHn0bZMhSiC4aYL1d{Bxem:2ZXnuJIV5eHKnc4Ppc44> NVTae|lTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2OlM4PjlpPkK1OlY{PzZ7PD;hQi=>
HepG2-HBV1.1 NV3aWHRVTnWwY4Tpc44hSXO|YYm= NYHFb4Y3OC5zIN88US=> M4LSdFI1KGh? Mnj6dJJwdW:2ZYOgbIVx[XSrdHnzJGIhfmm{dYOgdJJwfGWrbjDlfJBz\XO|aX;u M3rZTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nk[zO|Y6Lz5{NU[2N|c3QTxxYU6=
HepG2-HBV1.1 NYTtRVdkTnWwY4Tpc44hSXO|YYm= M2DGOlAvOSEQvF2= MUK0PEBp MorKdJJwdW:2ZYOgZ4VtdCCneHPy[ZRqd25ib3[gbIVx[XSrdHnzJGIhfmm{dYOgcpVkdGWxY3Hwd4lleyCrboP0[YFlKG:oIHjldIF1cXSrczDCJJZqenW|IFThcoUheGG{dHnjcIV{ NFewSVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[2N|c3QSd-MkW2OlM4Pjl:L3G+
HepG2-HBV1.1 NVTVOoZnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTOS|cxNjFizszN M4iwblEuPSCm MmnwbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36geIlu\SCmZYDlcoRmdnSueR?= NFPCPVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[2N|c3QSd-MkW2OlM4Pjl:L3G+
HepG2-HBV1.1 NHLyUXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjpTlFXOC5zIN88US=> MVixMVUh\A>? NVLhbHpEcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hfGmvZTDk[ZBmdmSnboTsfS=> M1n4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nk[zO|Y6Lz5{NU[2N|c3QTxxYU6=
HepG2-HBV1.1 MnqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjtcZY2OC5zIN88US=> MUWxNk04OiCq NUHvcVlwcW6mdXPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBifCCVLYDoZZNm MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ4M{e2PUc,OjV4NkO3Olk9N2F-
HepG2-HBV1.1 NHPJdY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHaPY9mOC5zIN88US=> M1jUUlEzNTd{IHi= M2HH[olv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgZZQhWy2yaHHz[S=> MmDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4NkO3OlkoRjJ3Nk[zO|Y6RC:jPh?=
Raji NHPPdXdHfW6ldHnvckBCe3OjeR?= MV2wMlXDqM7:TR?= MlvzO|IhcA>? NXrtWoxn[WKxbHnzbIV{KGG3dH;wbIFogcLi M3\q[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NES2N|c4Lz5{NUS0OlM4PzxxYU6=
SK-MEL-28  NUn0UXpDTnWwY4Tpc44hSXO|YYm= NIPqfZE{OCCwTR?= NUjISWVoPiCq M4XxeIlv\HWlZYOgS|LjiJOPIHPlcIwu[3mlbHWgZZJz\XO2 NY\4VlZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVMxOTBpPkK1N|E{ODFyPD;hQi=>
H157 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILFTJRKSzVyPUGuNFMhyrFiMD6wOEBucXV{TR?= MlrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NUe5NVEoRjJ3MkW3PVEyRC:jPh?=
Jurkat MofNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVW2PGNROS1|IH7N M3HpNVQ5yqCq MUXEUXNQ NETVNFFl\WO{ZXHz[ZMhfmmjYnzlJINmdGxiboXtZoVzeyCmb4PlJIRmeGWwZHXueIx6 NWi1UHpiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOVYyPDZpPkK1NVU3OTR4PD;hQi=>
CEM Mkn0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkKzNU0{KG6P Mli0OFjDqGh? MWfEUXNQ MmPK[IVkemWjc3XzJJZq[WKuZTDj[YxtKG63bXLldpMh\G:|ZTDk[ZBmdmSnboTsfS=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF3NkG0Okc,OjVzNU[xOFY9N2F-
P12 NU\5N5FKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHxNU0{KG6P M2jJSVQ5yqCq MkW0SG1UVw>? MUDk[YNz\WG|ZYOgeoli[mynIHPlcIwhdnWvYnXyd{Bld3OnIHTldIVv\GWwdHz5 M17qdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUW2NVQ3Lz5{NUG1OlE1PjxxYU6=
KB Mn36S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vSZ2lEPTB;MD6wNFQ6KMLzIECuNFAxOSEQvF2= Mnj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNUi1NlYoRjJ3MEW4OVI3RC:jPh?=
KBv200 (ABCB1) NGTOV2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjHTWM2OD1yLkO0OlIhyrFiMD6wNFY3KM7:TR?= MnXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNUi1NlYoRjJ3MEW4OVI3RC:jPh?=
SUDHL6  MkfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDudI4y6oDLzszN MXe0PE84OiCq M{PMbIlvcGmkaYTzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> MmHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkG2NFQoRjJ2OU[xOlA1RC:jPh?=
SUDHL6  M3LOOWZ2dmO2aX;uJGF{e2G7 M3[wTVHjiIoQvF2= NUe4SpcxPzJiaB?= MV;pcoR2[2W|IIPp[45q\mmlYX70JIFxd3C2b4TpZ{Bud3KyaH;sc4d6KGOqYX7n[ZMh[2:2cnXzeIVlKHerdHigRmNNOTGDIIPpVm5C NUHBSXdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlE3ODRpPkK0PVYyPjB2PD;hQi=>
HCT-116 NGH3RVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13MSmdKPTB;NTDuUS=> NH3MeGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmyO|g2Pyd-MkS5Nlc5PTd:L3G+
SKNBe2C NVHW[nh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTN{LklCtVQvOCCwTR?= NIrESmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmyNVkzOCd-MkS5NlE6OjB:L3G+
IGNR91 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTJ2LkRCtVEvPyCwTR?= Ml7wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MkG5NlAoRjJ2OUKxPVIxRC:jPh?=
SKNAS MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPheHM2UUN3ME2xMlXDuTBwMjDuUS=> M1TPelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUKxPVIxLz5{NEmyNVkzODxxYU6=
LAN1 NHzJN2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTJT3pGUUN3ME2yMlPDuTBwMjDuUS=> NUXRPI1yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NlE6OjBpPkK0PVIyQTJyPD;hQi=>
SHSY5Y NFXzSHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXVSZhHUUN3ME24MlLDuTBwNjDuUS=> NYn0O25ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NlE6OjBpPkK0PVIyQTJyPD;hQi=>
A549-WT M{LFbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{WwT2lEPTB;MUmuOFM4KMLzIECuOVk1KCCwTR?= NXThbXE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVg5OjdpPkK0PFU5QDJ5PD;hQi=>
A549-R NGLyVotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTBTZJXUUN3ME2xNk4yPzhiwsGgNE4{OzNiIH7N NXy1S5VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVg5OjdpPkK0PFU5QDJ5PD;hQi=>
MCF-7-WT Mn2wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrKTWM2OD1zNz63OVIhyrFiMD6yNVghKG6P NWTwOVhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVg5OjdpPkK0PFU5QDJ5PD;hQi=>
MCF-7-R MoXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHj[W5EUUN3ME21MlU{QSEEsTCwMlE1PCBibl2= Ml\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NUi4NlcoRjJ2OEW4PFI4RC:jPh?=
A549-R MYHDfZRwfG:6aXPpeJkhSXO|YYm= NFvQe|lKSzVyPUGyMlE4QCEEsTCwMlM{OyCwTR?= Ml33QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NUi4NlcoRjJ2OEW4PFI4RC:jPh?=
MCF-7-R MoGyR5l1d3SxeHnjbZR6KEG|c3H5 NHTyXJlKSzVyPUWuOVM6KMLzIECuNVQ1KG6P M3eyZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW4PFI4Lz5{NEi1PFgzPzxxYU6=
SW620 NX3uXJF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmiwTWM2OD15LkiwJOKyKDBwMEigcm0> Mnf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Mk[3N|koRjJ2N{K2O|M6RC:jPh?=
SW620/AD300 NIPuT45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTlyOT62NEDDuSB6LkmxJI5O NUjzfZhZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NlY4OzlpPkK0O|I3PzN7PD;hQi=>
HEK293/pcDNA3.1 M17rfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTFwNEWgxtEhOC5{MDDuUS=> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{NkezPUc,OjR5Mk[3N|k9N2F-
HEK293/ABCC1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTF7LkK5JOKyKDJwMEigcm0> NUDDO|ljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NlY4OzlpPkK0O|I3PzN7PD;hQi=>
TCC M{[5[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXGNXB3OjRiaB?= MoO2TWM2OD15MDDuUS=> MmqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MU[5OFQoRjJ2N{G2PVQ1RC:jPh?=
TCC Mk\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXq0PEBp M3LL[WlEPTB;NUCgcm0> NUi0So1KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVY6PDRpPkK0O|E3QTR2PD;hQi=>
HepG2/ADM NXrwc5FVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGK0Z4VKSzVyPUKuO|g3O8LzMD6yN|cyKM7:TR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdyNEW1Okc,OjR5MES1OVY9N2F-
HepG2 NUmyd5JHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTBwMEGxOeKyOC5yMEG3JO69VQ>? NGDQXXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEewOFU2Pid-MkS3NFQ2PTZ:L3G+
MCF-7/ADR MkTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojjTWM2OD12LkS4NlbDuTBwMkC3NEDPxE1? M37Mc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{C0OVU3Lz5{NEewOFU2PjxxYU6=
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A549/EGFP  MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LHc|AvODFvMUCwNEDPxE1? NIKwSmZKSzVyPUi2MlchyrFiMkmuNUDPxE1? NEnYPWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[zOFI5Oid-MkO2N|QzQDJ:L3G+
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Melanoma cells Mn\TRY51cXS3bX;yJIF{e2G7 NXT5XHdYSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdWWuYX7vcYEh[2WubIOsJGdKPTBiPTCwMlE2QDR7IN88UU4> MnXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3NUmyNFUoRjF5NUW5NlA2RC:jPh?=
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Melanoma cells MmTNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVPC[HhPT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hdWWuYX7vcYEh[2WubIOgZYZ1\XJiNEigbJJ{NCCJSUWwJF0hOC5zNjFOwG0v MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ6NEW5PUc,OjB4OES1PVk9N2F-
Melanoma cells MnXnR5l1d3SxeHnjbZR6KGG|c3H5 MoHsOFghcHK| M3PmUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJI1mdGGwb33hJINmdGy|IHHmeIVzKDR6IHjyd{whT0l3MDC9JFAvOTZizszNMi=> NUjvUolZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OFc4PDlpPkK0O|Q4PzR7PD;hQi=>
Melanoma cells M3P5VGN6fG:2b4jpZ4l1gSCjc4PhfS=> MkTwOFghcHK| NVn1eWRlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdWWuYX7vcYEh[2WubIOgZYZ1\XJiNEigbJJ{NCCJSUWwJF0hOC5zNjFOwG0v MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6M{SzNEc,OjJ{OEO0N|A9N2F-
A549 NYnJNHB7SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYDK[4syPDhiaILz M4fycmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6xO|E2KM7:TT6= M3\FWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEO4N|I{Lz5{OECzPFMzOzxxYU6=
NSCLC NYewfoMzSW62aYT1cY9zKGG|c3H5 MYS0PEBpenN? NG\sZnZCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOV2NNSyClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUSB;IECuNlUh|ryPLh?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTByNkK4OUc,OTlyME[yPFU9N2F-
NSCLC M{Hh[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV7qV2NtPDhiaILz M{PaR2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG5US0yFIHPlcIx{KGGodHXyJFQ5KGi{czygS2k2OCB;IECuNlUh|ryPLh?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ6NEW5PUc,OjB4OES1PVk9N2F-
NSCLC M1\aVWN6fG:2b4jpZ4l1gSCjc4PhfS=> M1rGTlQ5KGi{cx?= MkjKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUnNEVENiY3XscJMh[W[2ZYKgOFghcHK|LDDHTVUxKD1iMD6yOUDPxE1w M1\IfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizOFMxLz5{MkK4N|Q{ODxxYU6=
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NSCLC M4LyXGFvfGm2dX3vdkBie3OjeR?= MoG2RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgUnNEVENiY3XscJMtKEeLNUCgQUAxNjJ3MUG5JO69VS5? MkjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3NUmyNFUoRjF5NUW5NlA2RC:jPh?=
NSCLC NVS4c45ESW62aYT1cY9zKGG|c3H5 M1[xWFQ5KGi{cx?= NHvycXVCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOV2NNSyClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyIE2gNE4zPTFzOTFOwG0v MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTByNkK4OUc,OTlyME[yPFU9N2F-
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renal cancer cells NGL2OplCdnSrdIXtc5Ih[XO|YYm= NEDaTlM1QCCqcoO= NIn3eFhCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDy[Y5idCClYX7j[ZIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OCB;IECuN|E3OjNizszNMi=> NXjn[VlLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNFYzQDVpPkG5NFA3Ojh3PD;hQi=>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MPM2 / Cyclin B1 / Cdc25c / p-Cdc2 / Cdc2 / Aurora B / p-H3 / p-PLK1; 

PubMed: 26156322     


MOLT-4 Cells were treated with vincristine (0.3, 1, and 3 nM) alone or co-treated with SAHA (500 nM) for 24 h. Then, total cell lysates were obtained to evaluate the G2/M phase regulatory protein expression and the tubulin acetylation. Data are expressed as mean values ± SD of at least three separate determinations. S SAHA, V vincristine

26156322
Growth inhibition assay
Cell viability ; 

PubMed: 26156322     


MOLT-4 cells were treated with various concentrations of vincristine for 24 and 48 h, respectively.  Cell viability was measured by MTT assay.

26156322
体内研究 Vincristine 按3 mg/kg剂量单独腹腔注射给药携带双侧皮下移植瘤RH12或RH18的小鼠,平均生长延迟120天和52天以上,且再植指数分别为0.06%和5%。[5] Vincristine 处理小鼠,通过宿主细胞介导的血管效应及直接的微管蛋白介导的的细胞毒性作用而对皮下结肠38肿瘤起作用。Vincristine (5 mg/kg) 降低肿瘤血流量,降低近75%。[6]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
- 合并
  • Cell lines: B16 黑色素瘤细胞
  • Concentrations: 10 nM
  • Incubation Time: 3 天
  • Method: 细胞按5 × 104 cells/mL的浓度接种在含2 mL培养基的35-mm 实验板上,在37°C下 含5% CO2 和95% 空气 的环境下生长24小时。然后使用有或无10 nM药物的新鲜培养基更换原来的培养基,继续增殖3天。使用胰蛋白酶和EDTA将细胞分离后,每天使用库氏计数器完成细胞计数
    (Only for Reference)
动物实验:[5]
- 合并
  • Animal Models: 携带人类横纹肌肉瘤移植瘤Rh12的鼠
  • Dosages: 3 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (108.33 mM)
Water 60 mg/mL (65.0 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
Saline
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 923.04
化学式

C46H58N4O14S

CAS号 2068-78-2
储存条件 粉状
溶于溶剂
别名 Leurocristine, Oncovin, 22-Oxovincaleukoblastine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04448834 Suspended Drug: Blinatumomab B-cell Acute Lymphoblastic Leukemia Dorothy Sipkins MD PhD|Amgen|Acrotech Biopharma LLC|Duke University May 24 2021 Phase 2
NCT03981510 Recruiting Diagnostic Test: 3D-Transit Constipation|Childhood Acute Lymphoblastic Leukemia|Neurofibromatosis 1|Chronic Constipation With Overflow University of Aarhus June 13 2019 Not Applicable

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I want to use the product #S1421 for mouse in vivo, could you please give us your advice about administration method?

  • 回答:

    According to the reference cited on our website, we test the solubility of S1421 Staurosporine in 4% DMSO/saline, and it is a suspension at 5 mg/mL. The compound can also be dissolved in the vehicle 4% DMSO/30% PEG 300/10% Tween 80/saline at 5 mg/ml as a clear solution for injection.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID