NU7441 (KU-57788)

For research use only. Not for use in humans.

目录号:S2638

NU7441 (KU-57788) Chemical Structure

CAS No. 503468-95-9

NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制 mTORPI3K,对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 922.34 现货
RMB 565.91 现货
RMB 3040.41 现货
RMB 7928.18 现货
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客户使用Selleck生产的NU7441 (KU-57788)发表文献196篇:

产品安全说明书

DNA-PK抑制剂选择性比较

生物活性

产品描述 NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制 mTORPI3K,对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。
靶点
DNA-PK [1]
(Cell-free assay)
mTOR [1]
(cell-free assay)
PI3K [1]
(cell-free assay)
14 nM 1.7 μM 5 μM
体外研究

NU7441抑制DNA依赖性蛋白激酶 (DNA-PK) 比抑制其他PI3K相关激酶 (PIKK)家族成员选择性高,IC50 为14 nM。[1] 电离辐射诱导或Etoposide诱导 DNA 损伤后,1 μM NU7441提高γH2AX病灶的持久性。NU7441 浓度为 0.5 μM 或 1 μM时,作用于SW620 和 LoVo细胞,显著提高电离辐射,Etoposide, 和 Doxorubicin 诱导的G2-M累积增多。[2] NU7441浓度为1 μM时,引起 Doxorubicin和电离辐射诱导的DNA双链断裂的持久性,也稍微降低同源重组活性 DNA-PK充足的 M059-Fus-1和 DNA-PK缺陷的 M059 J人类肿瘤细胞。[3] NU7441浓度为 10 μM 时,作用于缺乏和表达polη的细胞,抑制UV诱导 RPA p34过度磷酸化。[4] NU7441 浓度为1 μM时,作用于慢性淋巴细胞性白血病细胞。提高Fludarabine诱导的γH2AX病灶水平,也相应地降低Fludarabine诱导的细胞死亡。[5] NU7441浓度为 1 μM时,作用于慢性淋巴细胞性白血病细胞,抑制Mitoxantrone诱导的DNA-PKcs自磷酸化和修复。[6]它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率 [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1770 M3LJO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnVdJdPUUN3ME2wMlAzOTR|IN88US=> M2i0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
A172 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEWxfXZKSzVyPUCuNVU{OTZizszN NV7BeXFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NCI-H526 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnizTWM2OD1yLkK4OFA4KM7:TR?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
HDLM-2 M4nYOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTBwM{KzOFUh|ryP NUjT[Y1zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
HuO-3N1 M2HibWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf6TWM2OD1yLkS2OlM3KM7:TR?= MnW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NCI-SNU-1 NX61cWplT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DMVmlEPTB;MD62NFk6PCEQvF2= MorNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
SK-N-AS MkLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPpTWM2OD1yLk[yNlE3KM7:TR?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
LoVo MnPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoCzTWM2OD1yLki2O|Q6KM7:TR?= M1e3flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
LAMA-84 M3TrdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwOUi4N|Mh|ryP NU\lTYlCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
CTB-1 NV3mUWE1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnvOWw4UUN3ME2xMlEyPTR4IN88US=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
KP-N-YS Mn:yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTFwMUSyN|Eh|ryP MnXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
HH NEnndGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDJWFFOUUN3ME2xMlE5ODl|IN88US=> Mmn3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
CHL-1 NWWw[GJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGOycJRKSzVyPUGuNlE{PjhizszN MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
NB7 NHLt[nhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PXNmlEPTB;MT6yOFc1PSEQvF2= NYfkdllmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
HL-60 MmLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGGx[otKSzVyPUGuN|gyQSEQvF2= NHLTSWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
639-V M2fTS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTFwNESxNlgh|ryP NHjlTHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NCI-H1793 M1zHUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fxPGlEPTB;MT60OVg1KM7:TR?= NVm3ZW9nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NEC8 NIXVe4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTFwNUWzOUDPxE1? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
PA-1 NHrQdmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDXTWM2OD1zLkW4Nlk1KM7:TR?= M17TXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
D-423MG NULtSI17T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn20TWM2OD1zLk[5N|YyKM7:TR?= M{jmO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
NCI-H292 M333[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PZeGlEPTB;MT63OFAyOyEQvF2= NWnWcFZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
SK-MEL-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHOcXBuUUN3ME2xMlc6PDd7IN88US=> M17HU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
LB2241-RCC NXrZbIk4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTJwMUSxOFYh|ryP NIHKRo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
SK-MEL-3 NX;PRlJJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlz0TWM2OD1{LkG2OFkh|ryP M2fLVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
MHH-PREB-1 NVrMfoE1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3ixfGlEPTB;Mj6xOlYxQSEQvF2= NYnYdFduRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
BFTC-905 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;YVo1KSzVyPUKuNVY3QDdizszN MkTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
Ramos-2G6-4C10 M1;MXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ftS2lEPTB;Mj6xPVkxQSEQvF2= MlnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
G-361 MlTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLETWM2OD1{LkKxOlgh|ryP NXTZWphSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
MOLT-4 Mme0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrHVHNKSzVyPUKuNlE5QDhizszN NHPX[nQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
DEL NFnVN2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLBTWM2OD1{LkO1OVY1KM7:TR?= MoTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
MV-4-11 M3jGemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXm4PY1SUUN3ME2yMlM4OzlizszN NWXYW4RXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
SK-NEP-1 NXLQW4tNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTJwM{i3PFMh|ryP NELiSFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
RS4-11 M{HySGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXUTWM2OD1{LkW2NFI{KM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
HuH-7 Ml;US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTkU3k6UUN3ME2yMlU3PzB{IN88US=> NXWwOlVmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
A101D NYDaXJQxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTJwNkGzN|gh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
KG-1 Ml;lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLBTWM2OD1{Lk[3PVI1KM7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
KALS-1 M13OUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jiV2lEPTB;Mj62PVY{OyEQvF2= NILHVnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
VMRC-RCZ NVTJflJrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zSN2lEPTB;Mj63O|MzQCEQvF2= NYjucJYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
SK-UT-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTPTWM2OD1|LkG1N|Eh|ryP MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
SW962 MoTKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTNwMkCzOVIh|ryP MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51; 

PubMed: 29344644     


SUNE1 cells were irradiated with 5 Gy in the presence or absence of NU7441 (2 µM). The expression of cell cycle checkpoint proteins and double strand break repair proteins was assayed by western blot analysis at 6 or 12 h post-IR.

p-mTOR(Ser2448) / mTOR / p-S6K(Thr389); 

PubMed: 25714019     


Phosphorylation of mTOR and p70S6K were detected by Western blot analysis 24 hours after treatment.

29344644 25714019
体内研究 NU7441按 10 mg/kg剂量腹腔注射处理携带SW620移植瘤的小鼠,按无毒剂量处理至少4小时,然后提高 Etoposide诱导的肿瘤生长延迟2倍。 [2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

- 合并
  • Cell lines: SW620, LoVo, V3-YAC和 V3细胞
  • Concentrations: 0.5 μM或 1 μM
  • Incubation Time: 17 小时
  • Method:

    通过克隆实验测定NU7441,Etoposide, Doxorubicin, 和电离辐射作用于SW620, LoVo, V3,和 V3-YAC细胞存活的效果。在有或无 NU7441(0.5 或 1.0 μM)存在时,使用Etoposide 或Doxorubicin处理6孔板或6-cm盘中指数生长的细胞16小时。放射增敏研究中, NU7441加到细胞中,1小时后,使用辐射处理。使用γ-射线 (3.1 Gy/min 137Cesium)处理V3和V3-YAC 细胞。使用可用设备,使用X-射线 (2.9 Gy/min at 230 kV, 10 mA) 处理SW620 和 LoVo 细胞。辐射处理后,在有或无NU7441存在时,细胞再温育16小时。胰蛋白酶消化,收集细胞,计数,然后按每盘 100 到105 的密度接种在10-cm 直径的Petri盘中, 盘中含无药物培养基,用于形成集落。使用结晶紫对集落进行染色,10 到 14天后,使用自动集落计数器计数。


    (Only for Reference)
动物实验:

[2]

- 合并
  • Animal Models: 携带 SW620移植瘤的雌性裸鼠
  • Dosages: 10 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL warmed (9.67 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
0.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 413.49
化学式

C25H19NO3S

CAS号 503468-95-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Could you please suggest formulations of the drug for in vivo injection?

  • 回答:

    We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

  • 问题 2:

    Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

  • 回答:

    Slight color variation due to different processing is normal and usually doesn't interfere with the function.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID