NU7441 (KU-57788)

目录号:S2638

仅限科研使用

NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制 mTORPI3K,对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。

NU7441 (KU-57788) Chemical Structure

CAS: 503468-95-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 922.34 现货
RMB 565.91 现货
RMB 3040.41 现货
RMB 7928.18 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的NU7441 (KU-57788)发表文献204篇:

产品安全说明书

DNA-PK抑制剂选择性比较

相关DNA-PK产品

生物活性

产品描述 NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制 mTORPI3K,对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。
靶点
DNA-PK [1]
(Cell-free assay)
mTOR [1]
(cell-free assay)
PI3K [1]
(cell-free assay)
14 nM 1.7 μM 5 μM
体外研究

NU7441抑制DNA依赖性蛋白激酶 (DNA-PK) 比抑制其他PI3K相关激酶 (PIKK)家族成员选择性高,IC50 为14 nM。[1] 电离辐射诱导或Etoposide诱导 DNA 损伤后,1 μM NU7441提高γH2AX病灶的持久性。NU7441 浓度为 0.5 μM 或 1 μM时,作用于SW620 和 LoVo细胞,显著提高电离辐射,Etoposide, 和 Doxorubicin 诱导的G2-M累积增多。[2] NU7441浓度为1 μM时,引起 Doxorubicin和电离辐射诱导的DNA双链断裂的持久性,也稍微降低同源重组活性 DNA-PK充足的 M059-Fus-1和 DNA-PK缺陷的 M059 J人类肿瘤细胞。[3] NU7441浓度为 10 μM 时,作用于缺乏和表达polη的细胞,抑制UV诱导 RPA p34过度磷酸化。[4] NU7441 浓度为1 μM时,作用于慢性淋巴细胞性白血病细胞。提高Fludarabine诱导的γH2AX病灶水平,也相应地降低Fludarabine诱导的细胞死亡。[5] NU7441浓度为 1 μM时,作用于慢性淋巴细胞性白血病细胞,抑制Mitoxantrone诱导的DNA-PKcs自磷酸化和修复。[6]它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率 [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1770 NV3kelZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTBwMEKxOFMh|ryP NFLBXHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
A172 NFz1RWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwMUWzNVYh|ryP NFPacW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NCI-H526 NHjvTmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjpRXhpUUN3ME2wMlI5PDB5IN88US=> M1XMXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
HDLM-2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwM{KzOFUh|ryP MnvzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
HuO-3N1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fDemlEPTB;MD60OlY{PiEQvF2= MoTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NCI-SNU-1 NGPNe3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfrTWM2OD1yLk[wPVk1KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-N-AS NIPNRmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LldGlEPTB;MD62NlIyPiEQvF2= MojJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
LoVo NUXXRm04T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XqT2lEPTB;MD64Olc1QSEQvF2= NUXwPWJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
LAMA-84 NWK0eIhnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnsZZhmUUN3ME2wMlk5QDN|IN88US=> NHTUTFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
CTB-1 NVjScnNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmL4TWM2OD1zLkGxOVQ3KM7:TR?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
KP-N-YS MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HqfGlEPTB;MT6xOFI{OSEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
HH MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfrN|FKSzVyPUGuNVgxQTNizszN Ml\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
CHL-1 NILtbWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzzS2lKUUN3ME2xMlIyOzZ6IN88US=> MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NB7 NIfNOI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;TTWM2OD1zLkK0O|Q2KM7:TR?= NHjOfGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
HL-60 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTFwM{ixPUDPxE1? NXXaZ5JnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
639-V NWTUeHQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnoTWM2OD1zLkS0NVI5KM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
NCI-H1793 NVTibm45T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\qUJpKSzVyPUGuOFU5PCEQvF2= Mn;WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NEC8 M1fDPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{frS2lEPTB;MT61OVM2KM7:TR?= NF\0RlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
PA-1 NXW5cpk3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1[zRmlEPTB;MT61PFI6PCEQvF2= Ml3tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
D-423MG M1PpTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXScGtZUUN3ME2xMlY6OzZzIN88US=> NY\OUnh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NCI-H292 NWfrS4NGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfXT|lZUUN3ME2xMlc1ODF|IN88US=> M3jHWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
SK-MEL-1 M2ixfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jDUmlEPTB;MT63PVQ4QSEQvF2= NUHEO|NKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
LB2241-RCC NXLrbXBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHFS2dKSzVyPUKuNVQyPDZizszN MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-MEL-3 M4qxeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUG4WHdWUUN3ME2yMlE3PDlizszN MkWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
MHH-PREB-1 NFfrN25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLDb3BLUUN3ME2yMlE3PjB7IN88US=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
BFTC-905 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTCZVhKSzVyPUKuNVY3QDdizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
Ramos-2G6-4C10 NFHMSGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;2N2lEPTB;Mj6xPVkxQSEQvF2= NXTze2RzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
G-361 NUDxWYJCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTJwMkG2PEDPxE1? MoLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
MOLT-4 NHzLeGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjsdHRMUUN3ME2yMlIyQDh6IN88US=> M4Kyb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
DEL M4[5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\XOFRjUUN3ME2yMlM2PTZ2IN88US=> M4Pqb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
MV-4-11 M{\xPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vsUWlEPTB;Mj6zO|M6KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-NEP-1 M{\DR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LBeGlEPTB;Mj6zPFc5OyEQvF2= NEjs[mE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
RS4-11 NXXx[pBRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37zeWlEPTB;Mj61OlAzOyEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
HuH-7 M2Lne2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLzTWM2OD1{LkW2O|AzKM7:TR?= NY\Ud5pTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
A101D MoTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTJwNkGzN|gh|ryP MkS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
KG-1 NHf4WZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fBbGlEPTB;Mj62O|kzPCEQvF2= M4PsSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
KALS-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGKz[oNKSzVyPUKuOlk3OzNizszN Mn;PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
VMRC-RCZ M3jtSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTJwN{ezNlgh|ryP M{\IZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
SK-UT-1 M4DPNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fnO2lEPTB;Mz6xOVMyKM7:TR?= NXywUHU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
SW962 NFu2OoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrRdmdKSzVyPUOuNlA{PTJizszN Mo\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
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NB-EBc1 NH;3c3RyUFSVIHHzd4F6 NE\ne4ZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> MofOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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INA-6 MmfVSpVv[3Srb36gZZN{[Xl? M4rxfVExKHWP MmPqOEBpenN? NGTPdWJFd3ewcnXneYxifGmxbjDv[kBPXlBvQWXZPVIzNWmwZIXj[YQhUFOILUGgdJJwfGWrbjDlfJBz\XO|aX;uJIlvKGi3bXHuJGlPSS14IHPlcIx{KGG2IEGwJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhPCCqcoOg[o9tdG:5ZXSgZpkhVl[SLVHVXVkzOiCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA1KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l MljwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUO5N|EoRjJ6NEWzPVMyRC:jPh?=
INA-6 NYHTZYVmTnWwY4Tpc44h[XO|YYm= MYixNEB2VQ>? NYraSmxSPCCqcoO= NYrTSWl6UW6qaXLpeIlwdiCxZjDOWnAuSVW\OUKyMYlv\HWlZXSgTHNHNTFicHjvd5Bpd3K7bHH0bY9vKGmwIHj1cYFvKEmQQT22JINmdGy|IHH0JFExKHWPIIDy[Ylv[3WkYYTl[EBnd3JiNDDodpMh\m:ubH;3[YQh[nliTm\QMWFWYTl{MjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m Ml7lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUO5N|EoRjJ6NEWzPVMyRC:jPh?=
INA-6 Ml\0SpVv[3Srb36gZZN{[Xl? NIOyTJQyOCC3TR?= M4PzclQhcHK| MYHEc5dvemWpdXzheIlwdiCxZjDOWnAuSVW\OUKyMYlv\HWlZXSgTHNHNTFibWLORUBmgHC{ZYPzbY9vKGmwIHj1cYFvKEmQQT22JINmdGy|IHH0JFExKHWPIIDy[Ylv[3WkYYTl[EBnd3JiNDDodpMh\m:ubH;3[YQh[nliTm\QMWFWYTl{MjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB2IHjyd{BjgSCUVD3QR3IhdWW2aH;k MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-
Assay
Methods Test Index PMID
Western blot p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51 p-mTOR(Ser2448) / mTOR / p-S6K(Thr389) 29344644 25714019
体内研究 NU7441按 10 mg/kg剂量腹腔注射处理携带SW620移植瘤的小鼠,按无毒剂量处理至少4小时,然后提高 Etoposide诱导的肿瘤生长延迟2倍。 [2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

  • Cell lines: SW620, LoVo, V3-YAC和 V3细胞
  • Concentrations: 0.5 μM或 1 μM
  • Incubation Time: 17 小时
  • Method:

    通过克隆实验测定NU7441,Etoposide, Doxorubicin, 和电离辐射作用于SW620, LoVo, V3,和 V3-YAC细胞存活的效果。在有或无 NU7441(0.5 或 1.0 μM)存在时,使用Etoposide 或Doxorubicin处理6孔板或6-cm盘中指数生长的细胞16小时。放射增敏研究中, NU7441加到细胞中,1小时后,使用辐射处理。使用γ-射线 (3.1 Gy/min 137Cesium)处理V3和V3-YAC 细胞。使用可用设备,使用X-射线 (2.9 Gy/min at 230 kV, 10 mA) 处理SW620 和 LoVo 细胞。辐射处理后,在有或无NU7441存在时,细胞再温育16小时。胰蛋白酶消化,收集细胞,计数,然后按每盘 100 到105 的密度接种在10-cm 直径的Petri盘中, 盘中含无药物培养基,用于形成集落。使用结晶紫对集落进行染色,10 到 14天后,使用自动集落计数器计数。

动物实验:

[2]

  • Animal Models: 携带 SW620移植瘤的雌性裸鼠
  • Dosages: 10 mg/kg
  • Administration: 腹腔注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O

0.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 413.49
化学式

C25H19NO3S

CAS号 503468-95-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

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常见问题及建议解决方法

问题 1:
Could you please suggest formulations of the drug for in vivo injection?

回答:
We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

问题 2:
Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

回答:
Slight color variation due to different processing is normal and usually doesn't interfere with the function.

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