NU7441 (KU-57788)

目录号:S2638

NU7441 (KU-57788) Chemical Structure

Molecular Weight(MW): 413.49

NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制PI3K,IC50为5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。

规格 价格 库存 购买数量  
RMB 565.91 现货
RMB 974.57 现货
RMB 3040.41 现货
RMB 7928.18 现货
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产品安全说明书

DNA-PK抑制剂选择性比较

生物活性

产品描述 NU7441 (KU-57788)是一种高度有效的,选择性DNA-PK抑制剂,在无细胞试验中IC50为14 nM,也会抑制PI3K,IC50为5 μM。它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率。
靶点
DNA-PK [1]
(Cell-free assay)
14 nM
体外研究

NU7441抑制DNA依赖性蛋白激酶 (DNA-PK) 比抑制其他PI3K相关激酶 (PIKK)家族成员选择性高,IC50 为14 nM。[1] 电离辐射诱导或Etoposide诱导 DNA 损伤后,1 μM NU7441提高γH2AX病灶的持久性。NU7441 浓度为 0.5 μM 或 1 μM时,作用于SW620 和 LoVo细胞,显著提高电离辐射,Etoposide, 和 Doxorubicin 诱导的G2-M累积增多。[2] NU7441浓度为1 μM时,引起 Doxorubicin和电离辐射诱导的DNA双链断裂的持久性,也稍微降低同源重组活性 DNA-PK充足的 M059-Fus-1和 DNA-PK缺陷的 M059 J人类肿瘤细胞。[3] NU7441浓度为 10 μM 时,作用于缺乏和表达polη的细胞,抑制UV诱导 RPA p34过度磷酸化。[4] NU7441 浓度为1 μM时,作用于慢性淋巴细胞性白血病细胞。提高Fludarabine诱导的γH2AX病灶水平,也相应地降低Fludarabine诱导的细胞死亡。[5] NU7441浓度为 1 μM时,作用于慢性淋巴细胞性白血病细胞,抑制Mitoxantrone诱导的DNA-PKcs自磷酸化和修复。[6]它可降低NHEJ的频率,而增强Cas9介导DNA剪接后发生的同源重组修复率 [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1770 Mom4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTBwMEKxOFMh|ryP MluxV2FPT0WU
A172 MnLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LtbWlEPTB;MD6xOVMyPiEQvF2= M3LJWnNCVkeHUh?=
NCI-H526 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moj6TWM2OD1yLkK4OFA4KM7:TR?= M2joUnNCVkeHUh?=
HDLM-2 NYjyc28{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\hVohtUUN3ME2wMlMzOzR3IN88US=> NV\ZVWdvW0GQR1XS
HuO-3N1 MnvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU[5W3NtUUN3ME2wMlQ3PjN4IN88US=> M3XK[3NCVkeHUh?=
NCI-SNU-1 MoO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFK2OlRKSzVyPUCuOlA6QTRizszN MUjTRW5ITVJ?
SK-N-AS MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7JZ5VsUUN3ME2wMlYzOjF4IN88US=> M1v2fnNCVkeHUh?=
LoVo MoHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HDdWlEPTB;MD64Olc1QSEQvF2= NXW2cJFzW0GQR1XS
LAMA-84 NYKyRllUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7rVI9KSzVyPUCuPVg5OzNizszN NUfuT|N6W0GQR1XS
CTB-1 NIqySnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnS5TWM2OD1zLkGxOVQ3KM7:TR?= NGLHO|JUSU6JRWK=
KP-N-YS MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTFwMUSyN|Eh|ryP NEPQdoJUSU6JRWK=
HH MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jsfGlEPTB;MT6xPFA6OyEQvF2= MoSxV2FPT0WU
CHL-1 NXvHZVAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjlS5VKSzVyPUGuNlE{PjhizszN NYX0Xmg{W0GQR1XS
NB7 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ntSGlEPTB;MT6yOFc1PSEQvF2= M1jCdXNCVkeHUh?=
HL-60 M4G4U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTFwM{ixPUDPxE1? MoXmV2FPT0WU
639-V MoXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTIT49KSzVyPUGuOFQyOjhizszN Mk\LV2FPT0WU
NCI-H1793 NIHyTnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml[1TWM2OD1zLkS1PFQh|ryP MVPTRW5ITVJ?
NEC8 M372bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTFwNUWzOUDPxE1? M1fCbXNCVkeHUh?=
PA-1 NF3UNmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvHdmRqUUN3ME2xMlU5Ojl2IN88US=> MlW1V2FPT0WU
D-423MG M{f3d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXPfoN2UUN3ME2xMlY6OzZzIN88US=> NVvVcWxuW0GQR1XS
NCI-H292 MnT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTFwN{SwNVMh|ryP M3HCeXNCVkeHUh?=
SK-MEL-1 NHP2NWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXVTWM2OD1zLke5OFc6KM7:TR?= MU\TRW5ITVJ?
LB2241-RCC NFHyO2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;jTWM2OD1{LkG0NVQ3KM7:TR?= M17qR3NCVkeHUh?=
SK-MEL-3 M4HGcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfsS3VoUUN3ME2yMlE3PDlizszN M4fZTnNCVkeHUh?=
MHH-PREB-1 NGq5XXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1H3RmlEPTB;Mj6xOlYxQSEQvF2= NXLIOIJ2W0GQR1XS
BFTC-905 NVnnc4Z3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LY[mlEPTB;Mj6xOlY5PyEQvF2= M2rCVHNCVkeHUh?=
Ramos-2G6-4C10 NITG[HVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTJwMUm5NFkh|ryP MVXTRW5ITVJ?
G-361 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTJwMkG2PEDPxE1? MmXQV2FPT0WU
MOLT-4 MlHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DsZWlEPTB;Mj6yNVg5QCEQvF2= NUHOW2R7W0GQR1XS
DEL NE\vN49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTJwM{W1OlQh|ryP MnPLV2FPT0WU
MV-4-11 NF7lfHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPxXWxKSzVyPUKuN|c{QSEQvF2= NXfiOlBJW0GQR1XS
SK-NEP-1 NYnEW5dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDnVWhbUUN3ME2yMlM5Pzh|IN88US=> MkjHV2FPT0WU
RS4-11 M1\CU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUK3Z49FUUN3ME2yMlU3ODJ|IN88US=> M4XzWXNCVkeHUh?=
HuH-7 NEjPb3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4Ts[mlEPTB;Mj61OlcxOiEQvF2= M2fkSXNCVkeHUh?=
A101D NXLpXol6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\1eYZKSzVyPUKuOlE{OzhizszN MYDTRW5ITVJ?
KG-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIq0SXpKSzVyPUKuOlc6OjRizszN NVrZWoZFW0GQR1XS
KALS-1 NFyyeHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDVSHFKSzVyPUKuOlk3OzNizszN MX7TRW5ITVJ?
VMRC-RCZ MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PGVGlEPTB;Mj63O|MzQCEQvF2= MnnDV2FPT0WU
SK-UT-1 M1\lfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXnTWM2OD1|LkG1N|Eh|ryP M4rUTnNCVkeHUh?=
SW962 NHToXIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfMXlhKSzVyPUOuNlA{PTJizszN MoDhV2FPT0WU
DBTRG-05MG MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTNwMkiwPFUh|ryP M{\TT3NCVkeHUh?=
PANC-08-13 M{jn[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LyZmlEPTB;Mz60NVI4QCEQvF2= NHXTO4tUSU6JRWK=
QIMR-WIL MmLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTNwNEGzOFEh|ryP MknJV2FPT0WU
SK-MEL-30 NXSwVVRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{W1TWlEPTB;Mz60NlU6QCEQvF2= MmC0V2FPT0WU
697 M4TCSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfBTWM2OD1|LkS0PVk{KM7:TR?= MYjTRW5ITVJ?
NY MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTNwNEWwNlIh|ryP NHyxN25USU6JRWK=
ES7 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlq1TWM2OD1|LkS2O|I6KM7:TR?= MmHzV2FPT0WU
JVM-3 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Dye2lEPTB;Mz60PFA4PyEQvF2= MmDWV2FPT0WU
U031 MnT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnITWM2OD1|LkW5N|Y5KM7:TR?= MnW4V2FPT0WU
BHT-101 M2rCUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLVZ3NKSzVyPUOuOVk6QDVizszN NUTPOm85W0GQR1XS
8305C NVvEW5VUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnKbWZbUUN3ME2zMlYyPzN4IN88US=> NGWzcGNUSU6JRWK=
MG-63 NHyyT25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTNwNkK2O|Yh|ryP MYLTRW5ITVJ?
NOS-1 MkHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LKVGlEPTB;Mz62OVMyOiEQvF2= NXTkd5dQW0GQR1XS
YH-13 Ml3vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\iXWFtUUN3ME2zMlg4OTh7IN88US=> NEn6epRUSU6JRWK=
LXF-289 NGjZUWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PITmlEPTB;Mz65NVA1OSEQvF2= MmjHV2FPT0WU
GOTO MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLQRnhKSzVyPUOuPVE1QSEQvF2= MX3TRW5ITVJ?
EKVX NWOx[FhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTRwMkK1NlQh|ryP NY\FeJc1W0GQR1XS
CAL-51 MlXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LKTGlEPTB;ND6yOFg2PCEQvF2= M{ixPXNCVkeHUh?=
AN3-CA MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PkSWlEPTB;ND6yOVgyOyEQvF2= M{XM[HNCVkeHUh?=
IA-LM M2\yTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17pd2lEPTB;ND6yO|k{PSEQvF2= MmTIV2FPT0WU
KU-19-19 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTRwM{K5NlEh|ryP NVr6SlBMW0GQR1XS
A704 NH7VdGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHETWM2OD12LkSwPFg5KM7:TR?= NV7GSFA{W0GQR1XS
RH-18 NGm2OoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LJSGlEPTB;ND60OFk2OyEQvF2= M{L2d3NCVkeHUh?=
IST-MEL1 M4Lscmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zNbWlEPTB;ND61PFI3OyEQvF2= NV;RdJBEW0GQR1XS
LU-134-A M3nrXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nOfGlEPTB;ND61PVY5PSEQvF2= MVrTRW5ITVJ?
SF126 MmLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTRwNkC3NVUh|ryP NF7jT5ZUSU6JRWK=
EFO-27 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzk[|kzUUN3ME20MlY3PTJ3IN88US=> NVHpcZhoW0GQR1XS
SN12C M1jPe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nFR2lEPTB;ND63NVg6QCEQvF2= Mlv2V2FPT0WU
L-363 MlXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHTTWM2OD12Lke3NVE3KM7:TR?= Mn\FV2FPT0WU
MEL-HO NIWzSGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zx[mlEPTB;ND63O|k5QSEQvF2= MYDTRW5ITVJ?
TYK-nu NULVNY9GT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fkUWlEPTB;ND64NFEzQCEQvF2= NELYSnNUSU6JRWK=
MDA-MB-415 M3rmS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHlTWM2OD13LkCwN|E1KM7:TR?= NELxbm5USU6JRWK=
HCC70 MorYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXWwNZRRUUN3ME21MlA{QTN7IN88US=> MnPYV2FPT0WU
IGROV-1 NGG4b2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jCT2lEPTB;NT6yOVI6OiEQvF2= M4PSfHNCVkeHUh?=
NCI-H1355 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEmzRlFKSzVyPUWuNlk3KM7:TR?= NXL3VldGW0GQR1XS
23132-87 M3jKbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTVwM{KwNlQh|ryP Mmi5V2FPT0WU
NB69 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoW0TWM2OD13LkOyOFIh|ryP MUjTRW5ITVJ?
HSC-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\wTWM2OD13LkSxO|c{KM7:TR?= MVHTRW5ITVJ?
KYSE-410 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fqZWlEPTB;NT60O|Y{PiEQvF2= M2HYZnNCVkeHUh?=
GCIY MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFH5UYRKSzVyPUWuOVE{PzhizszN NF:3epNUSU6JRWK=
A427 NE\vXZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWn3U5VQUUN3ME21MlU{PjZzIN88US=> MXLTRW5ITVJ?
ChaGo-K-1 MkTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTKS2g4UUN3ME21MlU3PTl7IN88US=> M{LER3NCVkeHUh?=
PFSK-1 M13CNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTVwNkKxPVQh|ryP M1LkRXNCVkeHUh?=
MLMA MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nmTmlEPTB;NT64N|YxPiEQvF2= M2jGc3NCVkeHUh?=
RVH-421 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1njZmlEPTB;Nj6wO|M{QCEQvF2= NVyzWoZ{W0GQR1XS
LB1047-RCC NUTZeVNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7u[JNKSzVyPU[uNVc2OjJizszN NUTlcHJQW0GQR1XS
MHH-NB-11 NU\5Om5QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWL5WIhuUUN3ME22MlMzPzJzIN88US=> Ml\WV2FPT0WU
J-RT3-T3-5 M{HKOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonmTWM2OD14Lk[zNlkyKM7:TR?= NID4RllUSU6JRWK=
UM-UC-3 NWLIbY1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHQOGlsUUN3ME22MlY1OzN2IN88US=> NYCwUnBMW0GQR1XS
IST-SL1 NG\3U5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTZwNkmzOlkh|ryP NF\OO2hUSU6JRWK=
NB14 NXvab3BnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTOTWM2OD14Lke5O|YyKM7:TR?= NEfHelZUSU6JRWK=
NBsusSR MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTZwOEK1NFIh|ryP M1nVSnNCVkeHUh?=
D-263MG M4fZfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn:2TWM2OD14LkmxNFY5KM7:TR?= NGnvRm9USU6JRWK=
NCI-H2347 M{O5N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTZwOUOyNVMh|ryP M1HMR3NCVkeHUh?=
ES8 M3\YVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnoTWM2OD14Lkm3OVg5KM7:TR?= MY\TRW5ITVJ?
786-0 MmjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrocnYxUUN3ME23MlA6Pzh4IN88US=> NEfmUY1USU6JRWK=
EPLC-272H Mo[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTdwMU[yOFYh|ryP MlLqV2FPT0WU
CAPAN-1 Mk\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorQTWM2OD15LkK0N|U6KM7:TR?= MknDV2FPT0WU
NCI-H82 M3LXNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTdwMk[2OVkh|ryP NEP4S|ZUSU6JRWK=
G-402 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPoTWM2OD15LkOwNFUyKM7:TR?= MoXxV2FPT0WU
SW982 M4ntPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXf4XoxnUUN3ME23MlMxOjB|IN88US=> M3GyZnNCVkeHUh?=
OVCAR-4 NHnYd3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTdwM{GzNlgh|ryP NVHUS2pDW0GQR1XS
KARPAS-299 M2jOSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\zUJNyUUN3ME23MlMzPjJ{IN88US=> MVLTRW5ITVJ?
MIA-PaCa-2 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X2[GlEPTB;Nz6zOlgzOyEQvF2= NVnjZYxqW0GQR1XS
AU565 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvaVHBiUUN3ME23MlU6QDN|IN88US=> MnfxV2FPT0WU
GAMG NVLRXnpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzVeIpUUUN3ME23MlY1OTNzIN88US=> NETRTYVUSU6JRWK=
HuO9 NH\FSXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jWUGlEPTB;Nz63NVc1OyEQvF2= MVvTRW5ITVJ?
ACN NX7R[3RIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTdwN{SzN{DPxE1? M4TUW3NCVkeHUh?=
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BALL-1 M3LOTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\JZ2lEPTB;MUKuNlY3OiEQvF2= NHr4bnhUSU6JRWK=
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Becker M4TYZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrZRnFKSzVyPUGzMlA2QCEQvF2= NED4SGlUSU6JRWK=
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NCI-H2030 NHrnR4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXOUZNEUUN3ME2xN{4zODZ{IN88US=> NW\0bo53W0GQR1XS
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CP50-MEL-B MkPzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTF|Lk[yN|Ih|ryP MXzTRW5ITVJ?
NCI-H1092 NUHUOm83T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvBTWM2OD1zMz63OFc2KM7:TR?= MX7TRW5ITVJ?
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CAL-27 NX\vW|lwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD5dZFzUUN3ME2xOE4xPzJ5IN88US=> MmHOV2FPT0WU
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D-566MG MlO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXmVG46UUN3ME2xOE44PzB4IN88US=> M1\1c3NCVkeHUh?=
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ME-180 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHz[INRUUN3ME2xOU41Pzh|IN88US=> NH\GZ5hUSU6JRWK=
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NCI-H1304 Ml64S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTF3Lki5Nlgh|ryP MnziV2FPT0WU
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RH-1 M1zSVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDNTWM2OD1zNj64NlUh|ryP MWLTRW5ITVJ?
HT-1376 NG\xSlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLGcFJEUUN3ME2xOk45PDR4IN88US=> MnHRV2FPT0WU
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TK10 M2HWUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrue4xxUUN3ME2xO{4xODd7IN88US=> Ml2zV2FPT0WU
SW684 M2G4eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\DTWM2OD1zNz6wNVU1KM7:TR?= MlLJV2FPT0WU
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NCI-H596 Mn3DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjYXGlJUUN3ME2xPU43OzlzIN88US=> MlzMV2FPT0WU
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SJSA-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTJyLkW2PFgh|ryP NELjU|ZUSU6JRWK=
RCC10RGB MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTJyLk[3NVkh|ryP NGDwfWFUSU6JRWK=
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NCI-H1048 Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTJzLkKyOVgh|ryP M3HSb3NCVkeHUh?=
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HCC2157 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTJzLkO2JO69VQ>? MWfTRW5ITVJ?
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RD NEnOWmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTJ{Lke1NVgh|ryP MofsV2FPT0WU
NCI-H522 NIrwUXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULOcIp2UUN3ME2yNk45PDNzIN88US=> NX3SbGpyW0GQR1XS
CGTH-W-1 M4TZT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFizUnBKSzVyPUKzMlA3PDFizszN NEnFe|RUSU6JRWK=
SK-LU-1 M17KTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTJ|LkS2PFMh|ryP NUfZeGQxW0GQR1XS
BB65-RCC Mnu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fuRmlEPTB;MkOuO|g3OyEQvF2= NUjQbYlEW0GQR1XS
HSC-3 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PxR2lEPTB;MkOuPFUzPyEQvF2= Ml3oV2FPT0WU
SK-MES-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\JTWM2OD1{ND6wNVYyKM7:TR?= M33keHNCVkeHUh?=
DB NIfFbo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfS[IxKSzVyPUK0MlExOjhizszN M2ToOHNCVkeHUh?=
HO-1-N-1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jkcWlEPTB;MkSuN|A6OyEQvF2= MVPTRW5ITVJ?
MKN1 NYDVO3lDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2C4N2lEPTB;MkSuOlgh|ryP MoWzV2FPT0WU
MN-60 NWeyPYhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTJ2Lki1NVkh|ryP MoLQV2FPT0WU
8505C MnnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfaTWM2OD1{NT6xPFQ2KM7:TR?= NF\YUWtUSU6JRWK=
COLO-320-HSR Ml7NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPucFZKSzVyPUK1MlIzODhizszN M1PEbHNCVkeHUh?=
BB30-HNC NE\pNoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDYTWM2OD1{NT6yOFg{KM7:TR?= NIm2d45USU6JRWK=
KM12 NXq5XplkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVS1OmtWUUN3ME2yOU42QDB5IN88US=> Mnv4V2FPT0WU
NB13 M3vFR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEP4b|JKSzVyPUK1MlU5QThizszN NXvSWlRbW0GQR1XS
HCC2998 M1jPPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{Dze2lEPTB;Mk[uNFI5OyEQvF2= NULGe2RTW0GQR1XS
U-118-MG NIPKXZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYH3XmNPUUN3ME2yOk4xQDB6IN88US=> NYjvbXZbW0GQR1XS
NB10 NEXiV2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTJ4LkKzOEDPxE1? M1PaW3NCVkeHUh?=
SK-LMS-1 M{PhbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fFXGlEPTB;MkeuNFcxPCEQvF2= M362ZnNCVkeHUh?=
SW1573 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLzcm5iUUN3ME2yO{4yOSEQvF2= NYr0PZc3W0GQR1XS
LB373-MEL-D Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTXW2tKSzVyPUK3MlEzQTRizszN M2HBc3NCVkeHUh?=
DK-MG Mn\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTJ5LkG4NVch|ryP NWnJWHNxW0GQR1XS
RMG-I Mk[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTJ5LkKwNlkh|ryP MX7TRW5ITVJ?
SW1088 NIKxXmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTJ5LkWwOFIh|ryP NH\CdlVUSU6JRWK=
SK-OV-3 MlzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTJ5Lke1PVMh|ryP NUK1elVFW0GQR1XS
KP-N-YN M1LEdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvlTWM2OD1{Nz64NFQ{KM7:TR?= MWHTRW5ITVJ?
RPMI-7951 NFvH[XpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTvTWM2OD1{OD6yOFU3KM7:TR?= MoPBV2FPT0WU
CaR-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TJd2lEPTB;MkiuOVU5OiEQvF2= MVrTRW5ITVJ?
MKN7 Ml\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTJ6LkW5OlQh|ryP NVG3XFVzW0GQR1XS
CHP-212 NF;Ob4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGiy[3lKSzVyPUK4Mlc1ODFizszN M2XpWXNCVkeHUh?=
KYSE-450 MmHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXNUFhKSzVyPUK4MlgxQTFizszN M17ienNCVkeHUh?=
RXF393 M1zFOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnNSlRpUUN3ME2yPU4zQTB4IN88US=> M4Hae3NCVkeHUh?=
P30-OHK MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTJ7LkOwOFgh|ryP MWrTRW5ITVJ?
Ca9-22 NUfOcnRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnrNZdRUUN3ME2yPU41Ojl7IN88US=> M1\NRXNCVkeHUh?=
RT-112 NYezdZdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2S5d2lEPTB;MkmuOFYyOSEQvF2= NWjhOpFFW0GQR1XS
ES1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTJ7Lki1OFQh|ryP MVXTRW5ITVJ?
HCE-4 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTJ7Lki3PFYh|ryP NHO1S5ZUSU6JRWK=
AM-38 MojVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{K3[mlEPTB;M{CuNFYxPiEQvF2= NH;DWFdUSU6JRWK=
AsPC-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkH0TWM2OD1|MD64NFU{KM7:TR?= MXXTRW5ITVJ?
TE-9 Mn7TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{X6ZWlEPTB;M{CuPFc{PyEQvF2= M{f3dHNCVkeHUh?=
HuP-T4 MmLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PmUGlEPTB;M{CuPVY{QCEQvF2= NWOxelF{W0GQR1XS
LB2518-MEL Mnz2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTNzLkC3OFQh|ryP M1fuVHNCVkeHUh?=
NCI-H1703 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTNzLkC5NFUh|ryP M{LXUXNCVkeHUh?=
NCI-H2228 NWPxbFNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGSxfnhKSzVyPUOxMlQ3PTFizszN Ml;6V2FPT0WU
SCC-4 MoLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDZTo1UUUN3ME2zNU46OThzIN88US=> MWPTRW5ITVJ?
KNS-81-FD NVzNO4dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mle1TWM2OD1|Mj6wNVI5KM7:TR?= M2LDZXNCVkeHUh?=
NTERA-S-cl-D1 M3zT[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTN{LkC3N|kh|ryP NXTFNVhZW0GQR1XS
Daoy NGDoVHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI[z[YtKSzVyPUOyMlA5OzhizszN NFKzSpFUSU6JRWK=
ES4 NWHEbJdpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXUVZZuUUN3ME2zNk4{QTJ{IN88US=> M1rEenNCVkeHUh?=
MHH-ES-1 NUC1VVNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTN{LkS4O|Ih|ryP NGDCO3ZUSU6JRWK=
C-33-A NFy2TGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;ETWM2OD1|Mj63O|I5KM7:TR?= MnvsV2FPT0WU
BHY NHHKeYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3zTWM2OD1|Mj65OFk2KM7:TR?= MUjTRW5ITVJ?
Detroit562 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTN|Lk[2OVch|ryP M37yXnNCVkeHUh?=
EW-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTN|LkmzNlIh|ryP M2XVXXNCVkeHUh?=
NCI-H1395 NWTzT5puT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPEUIJrUUN3ME2zOE4xPzl7IN88US=> NV;ze5dRW0GQR1XS
KM-H2 M3W5cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTN4LkS3OFMh|ryP MoKyV2FPT0WU
NCI-H520 NVXt[IZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{S1[GlEPTB;M{[uO|U1OSEQvF2= MlGyV2FPT0WU
D-392MG NHznfm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnqTWM2OD1|Nz6zN|Q1KM7:TR?= NHLPR3hUSU6JRWK=
NOMO-1 MkHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHOVGl3UUN3ME2zO{45ODh7IN88US=> MmTUV2FPT0WU
TE-6 NUTFSmdTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4ftWWlEPTB;M{iuNVI1KM7:TR?= MV;TRW5ITVJ?
HT-3 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTvTWM2OD1|OD60OFM3KM7:TR?= NF3iNppUSU6JRWK=
RPMI-8226 NEHQ[pdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPMTWM2OD1|OD65OVE6KM7:TR?= NEO3epVUSU6JRWK=
U251 M2fMZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTN7LkCxNlQh|ryP MWTTRW5ITVJ?
GCT MnnDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGe3VWVKSzVyPUO5MlE5PTJizszN MnK0V2FPT0WU
A431 MkDtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTN7LkG4PVQh|ryP M2nOOHNCVkeHUh?=
TE-12 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTN7LkK0OFgh|ryP NWj4bIN4W0GQR1XS
HMV-II MlTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DCWWlEPTB;M{muOVA1PyEQvF2= MmftV2FPT0WU
IST-MES1 MoizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XyPGlEPTB;NECuNVUxOiEQvF2= NVrxVYh[W0GQR1XS
SW13 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfabYxKSzVyPUSwMlUyQSEQvF2= NEm5TY9USU6JRWK=
SF295 NVG0cnZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonHTWM2OD12MD64NFY3KM7:TR?= NIXWWGhUSU6JRWK=
MDA-MB-157 NVnlVHM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLienFKSzVyPUSwMlk1PjJizszN MYTTRW5ITVJ?
TE-1 NVfyTXQxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETRS|hKSzVyPUSxMlg4OTRizszN NXTRT2VSW0GQR1XS
HuCCT1 M1zGeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlm3TWM2OD12Mj61Nlg6KM7:TR?= MXPTRW5ITVJ?
KYSE-510 MorPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3:5UGlEPTB;NEOuNVc2PCEQvF2= NX3TSXlPW0GQR1XS
NCI-H358 NW\YV4dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTR2LkCzNFQh|ryP M3y2b3NCVkeHUh?=
SW900 NGP1WHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTR2LkC3OVgh|ryP MY\TRW5ITVJ?
CAS-1 NGjMUJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\TS|ZKSzVyPUS0MlA6QTFizszN MlPsV2FPT0WU
TE-8 MmjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3K5WWlEPTB;NESuOVY2QCEQvF2= M4jZNXNCVkeHUh?=
P12-ICHIKAWA MkDBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHn1PXZKSzVyPUS0MlYyOTJizszN NGPIc5VUSU6JRWK=
YKG-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDwWXNwUUN3ME20OE44QDFizszN MkPJV2FPT0WU
SNU-C2B MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHubmtJUUN3ME20OE44QTB|IN88US=> NYO5UGdFW0GQR1XS
LCLC-103H MonzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4Xi[WlEPTB;NESuPFcxOyEQvF2= M{HBWXNCVkeHUh?=
U-87-MG Ml:zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTR3LkGwNlIh|ryP MUTTRW5ITVJ?
HAL-01 NWPPOYpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\jWYZPUUN3ME20OU4yOzd7IN88US=> NX3INXNUW0GQR1XS
OVCAR-8 NG\ZeJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrFTWM2OD12NT6zOVY1KM7:TR?= M3rURnNCVkeHUh?=
KYSE-150 M4j6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTR5LkGwOVIh|ryP NX;1R4hmW0GQR1XS
OE33 NFizUHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIK1S21KSzVyPUS3MlE1OjlizszN M4rBfXNCVkeHUh?=
CAL-62 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLJUXdKSzVyPUS3MlQxPjhizszN NVzv[IF2W0GQR1XS
TCCSUP NGnkdGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELOUoFKSzVyPUS3MlYzPjRizszN MUHTRW5ITVJ?
H4 M1u4Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTR5Lk[2NlEh|ryP NWHueINVW0GQR1XS
CP66-MEL MlS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;iTWM2OD12OD6wO|A1KM7:TR?= Mlv1V2FPT0WU
SNB75 NVPBenY4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrWN4hLUUN3ME20PE4yQDd2IN88US=> NUPOb2lyW0GQR1XS
Mo-T MkDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3LTWM2OD12OD6yO|kh|ryP MnPnV2FPT0WU
BT-549 MkTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnm4TWM2OD12OD6zO|I6KM7:TR?= MWLTRW5ITVJ?
IGR-1 Mn;IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PFbGlEPTB;NEiuOlY1PSEQvF2= NIXscnNUSU6JRWK=
NCI-H1299 NFfSUGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjCV5NFUUN3ME20PE44OjR6IN88US=> M{TPNXNCVkeHUh?=
ALL-PO NH;iVXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3YcJVKSzVyPUS5MlkzPjhizszN M1fJdXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51; 

PubMed: 29344644     


SUNE1 cells were irradiated with 5 Gy in the presence or absence of NU7441 (2 µM). The expression of cell cycle checkpoint proteins and double strand break repair proteins was assayed by western blot analysis at 6 or 12 h post-IR.

p-mTOR(Ser2448) / mTOR / p-S6K(Thr389); 

PubMed: 25714019     


Phosphorylation of mTOR and p70S6K were detected by Western blot analysis 24 hours after treatment.

29344644 25714019
体内研究 NU7441按 10 mg/kg剂量腹腔注射处理携带SW620移植瘤的小鼠,按无毒剂量处理至少4小时,然后提高 Etoposide诱导的肿瘤生长延迟2倍。 [2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

+ 展开
  • Cell lines: SW620, LoVo, V3-YAC和 V3细胞
  • Concentrations: 0.5 μM或 1 μM
  • Incubation Time: 17 小时
  • Method:

    通过克隆实验测定NU7441,Etoposide, Doxorubicin, 和电离辐射作用于SW620, LoVo, V3,和 V3-YAC细胞存活的效果。在有或无 NU7441(0.5 或 1.0 μM)存在时,使用Etoposide 或Doxorubicin处理6孔板或6-cm盘中指数生长的细胞16小时。放射增敏研究中, NU7441加到细胞中,1小时后,使用辐射处理。使用γ-射线 (3.1 Gy/min 137Cesium)处理V3和V3-YAC 细胞。使用可用设备,使用X-射线 (2.9 Gy/min at 230 kV, 10 mA) 处理SW620 和 LoVo 细胞。辐射处理后,在有或无NU7441存在时,细胞再温育16小时。胰蛋白酶消化,收集细胞,计数,然后按每盘 100 到105 的密度接种在10-cm 直径的Petri盘中, 盘中含无药物培养基,用于形成集落。使用结晶紫对集落进行染色,10 到 14天后,使用自动集落计数器计数。


    (Only for Reference)
动物实验:

[2]

+ 展开
  • Animal Models: 携带 SW620移植瘤的雌性裸鼠
  • Formulation: 无菌 0.9% NaCl溶液
  • Dosages: 10 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL warmed (9.67 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
2mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 413.49
化学式

C25H19NO3S

CAS号 503468-95-9
储存条件 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

  • 问题 1:

    Could you please suggest formulations of the drug for in vivo injection?

  • 回答:

    We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

  • 问题 2:

    Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

  • 回答:

    Slight color variation due to different processing is normal and usually doesn't interfere with the function.

DNA-PK Signaling Pathway Map

DNA-PK Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID