NU7441 (KU-57788)

For research use only. Not for use in humans.

目录号：S2638

CAS No. 503468-95-9

NU7441 (KU-57788)是一种高度有效的，选择性DNA-PK抑制剂，在无细胞试验中IC50为14 nM，也会抑制 mTOR 和 PI3K，对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率，而增强Cas9介导DNA剪接后发生的同源重组修复率。

规格价格库存购买数量

10mM (1mL in DMSO) RMB 922.34 现货

5mg RMB 565.91 现货

50mg RMB 3040.41 现货

200mg RMB 7928.18 现货

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客户使用Selleck生产的NU7441 (KU-57788)发表文献147篇：

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Cell Res, 2018, 28(7):756-770

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Nat Commun, 2018, 9(1):5351

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Cell Rep, 2018, 24(4):987-1000

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Int J Cancer, 2018,

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Cell Death Dis, 2018, 9(9):931

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FASEB J, 2018, 32(4):1818-1829

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Mol Cancer Ther, 2018, 17(12):2551-2563

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DNA Repair (Amst), 2018, 68:68-74

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Mol Biol Cell, 2018, 29(15):1786-1797

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Int J Mol Sci, 2018,

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Sci Rep, 2018, 8(1):9685

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Sci Rep, 2018, 8(1):14421

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Biochem Biophys Rep, 2018, 16:115-121

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Oncotarget, 2018, 9(9):8560-8572

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JCI Insight, 2018, 3(3)98096

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Nucleic Acids Res, 2017, 45(1):244-254

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Cancer Immunol Res, 2017, 5(9):790-803

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Cancer Res, 2017, 77(17):4567-4578

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Cancer Res, 2017, 77(18):4745-4754

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J Cell Biol, 2017, 216(3):623-639

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EMBO Rep, 2017, 18(8):1412-1428

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Nat Cell Biol, 2016, 18(11):1196-1207

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blood, 2016, 10.1182/blood-2016-02-700328

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Blood, 2016, 128(4):574-83

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Nat Commun, 2016, 7:12880

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PLoS One, 2016, 11(12):e0167454

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产品安全说明书

DNA-PK抑制剂选择性比较

生物活性

产品描述 NU7441 (KU-57788)是一种高度有效的，选择性DNA-PK抑制剂，在无细胞试验中IC50为14 nM，也会抑制 mTOR 和 PI3K，对应的IC50值分别为1.7 μM和5 μM。它可降低NHEJ的频率，而增强Cas9介导DNA剪接后发生的同源重组修复率。

靶点

DNA-PK ^[1]
(Cell-free assay) mTOR ^[1]
(cell-free assay) PI3K ^[1]
(cell-free assay)

14 nM 1.7 μM 5 μM

体外研究
NU7441抑制DNA依赖性蛋白激酶 (DNA-PK) 比抑制其他PI3K相关激酶 (PIKK)家族成员选择性高，IC50 为14 nM。^[1] 电离辐射诱导或Etoposide诱导 DNA 损伤后，1 μM NU7441提高γH2AX病灶的持久性。NU7441 浓度为 0.5 μM 或 1 μM时，作用于SW620 和 LoVo细胞，显著提高电离辐射，Etoposide, 和 Doxorubicin 诱导的G2-M累积增多。^[2] NU7441浓度为1 μM时，引起 Doxorubicin和电离辐射诱导的DNA双链断裂的持久性，也稍微降低同源重组活性 DNA-PK充足的 M059-Fus-1和 DNA-PK缺陷的 M059 J人类肿瘤细胞。^[3] NU7441浓度为 10 μM 时，作用于缺乏和表达polη的细胞，抑制UV诱导 RPA p34过度磷酸化。^[4] NU7441 浓度为1 μM时，作用于慢性淋巴细胞性白血病细胞。提高Fludarabine诱导的γH2AX病灶水平，也相应地降低Fludarabine诱导的细胞死亡。^[5] NU7441浓度为 1 μM时，作用于慢性淋巴细胞性白血病细胞，抑制Mitoxantrone诱导的DNA-PKcs自磷酸化和修复。^[6]它可降低NHEJ的频率，而增强Cas9介导DNA剪接后发生的同源重组修复率 ^[7]。

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID

NCI-H1770 NHu4bo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXNTWM2OD1yLkCyNVQ{KM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

A172 MknkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLMTodZUUN3ME2wMlE2OzF4IN88US=> MmHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

NCI-H526 M2f4U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrxcWlKSzVyPUCuNlg1ODdizszN M1roRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

HDLM-2 NVf1WlA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnVTWM2OD1yLkOyN|Q2KM7:TR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

HuO-3N1 Ml6wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEf2SmpKSzVyPUCuOFY3OzZizszN NH;mXFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

NCI-SNU-1 M1XhRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHX3U3ZKSzVyPUCuOlA6QTRizszN NYrydZhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

SK-N-AS M2rxbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFmwWm5KSzVyPUCuOlIzOTZizszN M3npZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

LoVo NI[2NINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorQTWM2OD1yLki2O|Q6KM7:TR?= NFjsNW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

LAMA-84 MmfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfPVnlKSzVyPUCuPVg5OzNizszN NY\POItDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

CTB-1 NGjMbm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTFwMUG1OFYh|ryP MmfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

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NCI-H28 NFjIb21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYH6UXoyUUN3ME24MlE3QTZ{IN88US=> Ml;XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

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CHP-134 MorVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoOzTWM2OD16LkK0NVg2KM7:TR?= NH3sTXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

HT-1080 MoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfvZ|dKSzVyPUiuNlUxQTNizszN NXPNSo1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

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CAKI-1 NH7CcoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjyc|ZKSzVyPUmuNVY3OSEQvF2= NHz1bW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

HCC1937 NF\Ne3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\Kb4p7UUN3ME25MlY6QDZ3IN88US=> MoHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

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DU-4475 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\DbmlEPTB;OT65Olk{QSEQvF2= NFPmbXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

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NCI-H1437 MkXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF:3RmxKSzVyPUGwMlU4OjVizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

VM-CUB-1 MkDtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTFyLk[xOlQh|ryP MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

A2058 NFjWcZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTFyLk[0NFQh|ryP NGPTVWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

BPH-1 Moj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\GcWlEPTB;MUCuO|E1PSEQvF2= NELTdmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

NB5 NV\QVIs1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTFyLke1OVkh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

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CAL-72 Ml20S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHGxRnRKSzVyPUGwMlgxQTRizszN M{nlWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

SK-MEL-28 M2PGUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnS1TWM2OD1zMD64NlAzKM7:TR?= M{LORVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

C32 M4C2SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTFyLkizOFch|ryP M4Gw[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

NCI-H1155 M1uzVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTFyLkizO|Yh|ryP NWLoT5FiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

BB49-HNC MkD4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfOV|NKSzVyPUGwMlg2QDZizszN MkjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

GMS-10 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\STWM2OD1zMD64PVcyKM7:TR?= M{e0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

K5 MnPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTFyLkmwO|Eh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

OS-RC-2 NXmzOJpGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NES0VIdKSzVyPUGxMlA2PTlizszN NYLKZlVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

HTC-C3 NInrb2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnkTZdKSzVyPUGxMlA4OTRizszN MkDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

LB996-RCC MkmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\QTWM2OD1zMT6wO|I{KM7:TR?= M{TGSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

NCI-H1563 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTFzLkGwNVEh|ryP MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

CAL-54 NE[3TGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlv0TWM2OD1zMT60PFEyKM7:TR?= NWPqcnB[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

WM-115 M1O3Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPPVGhKSzVyPUGxMlY5OjRizszN MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

COLO-684 NX3iN4hkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTFzLkeyOlch|ryP M4e2W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

HOP-62 NGK0b21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF2wdG9KSzVyPUGxMlc2ODhizszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

NCI-H650 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml76TWM2OD1zMT64PVYyKM7:TR?= NYTUSXRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

TE-11 M4q2fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIr2S|NKSzVyPUGxMlk{OTVizszN Mlm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

COLO-679 M{foc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGOxR2FKSzVyPUGxMlk1PiEQvF2= Ml7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

FTC-133 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfDTWpEUUN3ME2xNk4xODd4IN88US=> NWq0dGh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

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NCI-H596 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrTTWM2OD1zOT62N|kyKM7:TR?= M{nI[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

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D-336MG MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLTTWM2OD1zOT65N|IzKM7:TR?= Mn7oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

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HEC-1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1faRmlEPTB;MkCuOFk4QCEQvF2= Mn7XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

EW-13 NUHrWo9qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrmRVhKSzVyPUKwMlU1PDNizszN NX3BNGlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

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RCC10RGB MlLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDiVWRrUUN3ME2yNE43PzF7IN88US=> NGK1U3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

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HCC2157 NIj0fWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHoe|l3UUN3ME2yNU4{PiEQvF2= M2XtdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

EW-22 MkD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDYTWM2OD1{MT6zO|Yh|ryP MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

A253 NVjWfoR3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljmTWM2OD1{MT60OFgyKM7:TR?= NHe5fnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

BE-13 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHwTWM2OD1{MT62NVE{KM7:TR?= M4n6O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

KINGS-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PGSmlEPTB;MkGuOlMyPiEQvF2= M4TSdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

ETK-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjpNVdKSzVyPUKxMlgyPTJizszN M{TQUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

NCI-H2452 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4[ze2lEPTB;MkGuPFY1PyEQvF2= M3P4eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

HPAF-II Mk\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTJzLki5NlMh|ryP NHi1PG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

D-542MG NIHnSnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLmTWM2OD1{MT65NFY6KM7:TR?= M{j0NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

NCI-H630 MnvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkiyTWM2OD1{Mj6wNlk3KM7:TR?= NVHJR2VtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

TGBC11TKB MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DVRmlEPTB;MkKuNFQ6KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

BCPAP NInOcFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHudFFKSzVyPUKyMlYyOSEQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

HLE NVXOd2tIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HtTGlEPTB;MkKuOlk4KM7:TR?= M1n0eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

NCI-H727 NF3SSnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7tTWM2OD1{Mj63N|Uh|ryP M2GxWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

RD MlKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;3XZNKSzVyPUKyMlc2OThizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

NCI-H522 MojiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTJ{Lki0N|Eh|ryP NYDzRmtmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

CGTH-W-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTJ|LkC2OFEh|ryP Mnv4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

SK-LU-1 NYqyV3pwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTJ|LkS2PFMh|ryP NVHDfmJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

BB65-RCC M3S5SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGm1OGJKSzVyPUKzMlc5PjNizszN NX7vPYM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?

HSC-3 M17uOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTJ|Lki1Nlch|ryP M1L0ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

SK-MES-1 NGLtT4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPzbJVYUUN3ME2yOE4xOTZzIN88US=> M{i0OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

DB M4\qWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTJ2LkGwNlgh|ryP MoHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

HO-1-N-1 M{nkeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTJ2LkOwPVMh|ryP M1K0U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>

MKN1 NYfI[IJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkO5TWM2OD1{ND62PEDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

MN-60 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXn2d3hDUUN3ME2yOE45PTF7IN88US=> NH3Wdnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

8505C NV\XWnBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPpTWM2OD1{NT6xPFQ2KM7:TR?= MofHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

COLO-320-HSR M1zpRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTJ3LkKyNFgh|ryP MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

BB30-HNC M1zOXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojBTWM2OD1{NT6yOFg{KM7:TR?= Ml3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

KM12 NUnp[JRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3KzOWlEPTB;MkWuOVgxPyEQvF2= NIXEUGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

NB13 MoPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHWXmxKSzVyPUK1MlU5QThizszN NF7rVpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,

HCC2998 NYTl[4pnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLZZ|VKSzVyPUK2MlAzQDNizszN MkG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

U-118-MG NEDV[pFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTJ4LkC4NFgh|ryP MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+

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SK-LMS-1 NHjvN3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLDSmJ5UUN3ME2yO{4xPzB2IN88US=> MorGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>

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NB-EBc1 NIq5UlZyUFSVIHHzd4F6 MUnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= M{\QVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=

LAN-5 MUfxTHRUKGG|c3H5 M2KxN5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= MkPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

Rh18 NGK0d5JyUFSVIHHzd4F6 NFfESWFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= Mn\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

INA-6 NIiwTlJHfW6ldHnvckBie3OjeR?= MnyxNVAhfU1? NI\qd4E1KGi{cx?= NVfac|NOUW6qaXLpeIlwdiCxZjDo[YF1KHOqb3PrJJJme3CxboPlJIlvKGi3bXHuJGlPSS14IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBJW0ZvMTDt[YRq[XSnZDD1dJJm\3WuYYTpc44hd2ZidHjlJGhUWDd{IIDyc5RmcW5iZYjwdoV{e2mxbjDheEAyOCC3TTDwdoVqdmO3YnH0[YQh\m:{IESgbJJ{KG[xbHzve4VlKGK7IFjTVFkxKGmwaHnibZRweiCjbnSgTHNTKGmwZIXj[ZIhVl[SLVHVXVkzOiCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA1KGi{ M1rmWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEWzPVMyLz5{OES1N|k{OTxxYU6=

INA-6 NXq0OWlkTnWwY4Tpc44h[XO|YYm= MX2xNEB2VQ>? M3jsdVQhcHK| MnjZTY5pcWKrdHnvckBw\iCqZXH0JJNpd2OtIILld5BwdnOnIHnuJIh2dWGwIFnORU03KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDIV2YuOSCvZXTpZZRm\CC3cILl[5Vt[XSrb36gc4YhfGinIFjTVFczKG2UTlGg[ZhxemW|c3nvckBifCBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JGhUWDlyIHnubIljcXSxcjDhcoQhUFOUIHnu[JVk\XJiTm\QMWFWYTl{MjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB2IHjyd{Bj MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-

INA-6 MnL6SpVv[3Srb36gZZN{[Xl? MXOxNEB2VQ>? MlLXOEBpenN? NXTl[VhQTG:5boLl[5Vt[XSrb36gc4YhVl[SLVHVXVkzOi2rbnT1Z4VlKEiVRj2xJJBzd3SnaX6g[ZhxemW|c3nvckBqdiCqdX3hckBKVkFvNjDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KE6YUD3BWXk6OjJiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-

INA-6 NVXSd3FbTnWwY4Tpc44h[XO|YYm= MkjINVAhfU1? NVfrN2t3PCCqcoO= M{D3bGlvcGmkaYTpc44hd2ZiTm\QMWFWYTl{Mj3pcoR2[2WmIFjTSk0yKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBKVkFvNjDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KE6YUD3BWXk6OjJiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? M{eyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEWzPVMyLz5{OES1N|k{OTxxYU6=

INA-6 NEPqeYxHfW6ldHnvckBie3OjeR?= NIj5fVcyOCC3TR?= MYe0JIhzew>? NVHLe2NNTG:5boLl[5Vt[XSrb36gc4YhVl[SLVHVXVkzOi2rbnT1Z4VlKEiVRj2xJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDJUmEuPiClZXzsd{BifCBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JG5XWC2DVWm5NlIh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgVnQuWEOUIH3leIhw\A>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods Test Index PMID

Western blot
p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51;

PubMed: 29344644      Oncol Rep

SUNE1 cells were irradiated with 5 Gy in the presence or absence of NU7441 (2 µM). The expression of cell cycle checkpoint proteins and double strand break repair proteins was assayed by western blot analysis at 6 or 12 h post-IR.

p-mTOR(Ser2448) / mTOR / p-S6K(Thr389);

PubMed: 25714019      Oncotarget

Phosphorylation of mTOR and p70S6K were detected by Western blot analysis 24 hours after treatment.

29344644 25714019

体内研究 NU7441按 10 mg/kg剂量腹腔注射处理携带SW620移植瘤的小鼠，按无毒剂量处理至少4小时，然后提高 Etoposide诱导的肿瘤生长延迟2倍。 ^[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验：
^[2]

- 合并

Cell lines: SW620, LoVo, V3-YAC和 V3细胞

Concentrations: 0.5 μM或 1 μM

Incubation Time: 17 小时

Method:
通过克隆实验测定NU7441，Etoposide, Doxorubicin, 和电离辐射作用于SW620, LoVo, V3,和 V3-YAC细胞存活的效果。在有或无 NU7441(0.5 或 1.0 μM)存在时，使用Etoposide 或Doxorubicin处理6孔板或6-cm盘中指数生长的细胞16小时。放射增敏研究中， NU7441加到细胞中，1小时后，使用辐射处理。使用γ-射线 (3.1 Gy/min ¹³⁷Cesium)处理V3和V3-YAC 细胞。使用可用设备，使用X-射线 (2.9 Gy/min at 230 kV, 10 mA) 处理SW620 和 LoVo 细胞。辐射处理后，在有或无NU7441存在时，细胞再温育16小时。胰蛋白酶消化，收集细胞，计数，然后按每盘 100 到10⁵ 的密度接种在10-cm 直径的Petri盘中，盘中含无药物培养基，用于形成集落。使用结晶紫对集落进行染色，10 到 14天后，使用自动集落计数器计数。

(Only for Reference)

动物实验：
^[2]

- 合并

Animal Models: 携带 SW620移植瘤的雌性裸鼠

Dosages: 10 mg/kg

Administration: 腹腔注射
(Only for Reference)

参考文献

[1] Leahy JJ, et al. Bioorg Med Chem Lett, 2004, 14(24), 6083-6087.

[2] Zhao Y, et al. Cancer Res, 2006 , 66(10), 5354-5362.

[3] Tavecchio M, et al. Cancer Chemother Pharmacol, 2012, 69(1), 155-164.

[4] Cruet-Hennequart S, et al. DNA Repair (Amst), 2006, 5(4), 491-504.

[5] Willmore E, et al. Clin Cancer Res, 2008, 14(12), 3984-3992.

[6] Elliott SL, et al. Br J Haematol, 2011, 152(1), 61-71.

[7] Robert F, et al. Genome Med. 2015, 7:93.

- 合并

溶解度 (25°C)

体外 DMSO 4 mg/mL warmed (9.67 mM)

Water Insoluble

Ethanol Insoluble

体内 从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)：
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
2mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download NU7441 (KU-57788) SDF

分子量 413.49

化学式
C₂₅H₁₉NO₃S

CAS号 503468-95-9

储存条件 3年-20°C 粉状
2年-80°C 溶于溶剂

别名 N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O %DMSO+ %

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

体内配方配制方法：取 μL DMSO母液，加入 μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
           2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量

=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2

×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释

初始浓度:

稀释倍数:

计算结果

C1=C0/X C1: LOG(C1):

C2=C1/X C2: LOG(C2):

C3=C2/X C3: LOG(C3):

C4=C3/X C4: LOG(C4):

C5=C4/X C5: LOG(C5):

C6=C5/X C6: LOG(C6):

C7=C6/X C7: LOG(C7):

C8=C7/X C8: LOG(C8):

分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量：

总分子量：g/mol

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量浓度体积分子量

= × ×

计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

* 必填项

* 姓名：

* 邮箱：

  电话：

* 留言内容：

常见问题及建议解决方法

问题 1：
Could you please suggest formulations of the drug for in vivo injection?

回答：
We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

问题 2：
Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

回答：
Slight color variation due to different processing is normal and usually doesn't interfere with the function.

细胞实验： ^[2]	- 合并 Cell lines: SW620, LoVo, V3-YAC和 V3细胞 Concentrations: 0.5 μM或 1 μM Incubation Time: 17 小时 Method: 通过克隆实验测定NU7441，Etoposide, Doxorubicin, 和电离辐射作用于SW620, LoVo, V3,和 V3-YAC细胞存活的效果。在有或无 NU7441(0.5 或 1.0 μM)存在时，使用Etoposide 或Doxorubicin处理6孔板或6-cm盘中指数生长的细胞16小时。放射增敏研究中， NU7441加到细胞中，1小时后，使用辐射处理。使用γ-射线 (3.1 Gy/min ¹³⁷Cesium)处理V3和V3-YAC 细胞。使用可用设备，使用X-射线 (2.9 Gy/min at 230 kV, 10 mA) 处理SW620 和 LoVo 细胞。辐射处理后，在有或无NU7441存在时，细胞再温育16小时。胰蛋白酶消化，收集细胞，计数，然后按每盘 100 到10⁵ 的密度接种在10-cm 直径的Petri盘中，盘中含无药物培养基，用于形成集落。使用结晶紫对集落进行染色，10 到 14天后，使用自动集落计数器计数。 (Only for Reference)
动物实验： ^[2]	- 合并 Animal Models: 携带 SW620移植瘤的雌性裸鼠 Dosages: 10 mg/kg Administration: 腹腔注射 (Only for Reference)

体外	DMSO	4 mg/mL warmed (9.67 mM)
Water	Insoluble
Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 5% DMSO+30% PEG 300+5% Tween 80+ddH2O	2mg/mL

分子量	413.49
化学式	C₂₅H₁₉NO₃S
CAS号	503468-95-9
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

DNA-PK Signaling Pathway Map

DNA-PK Inhibitors with Unique Features

非特异性的DNA-PK抑制剂

PI-103 : DNA-PK, IC50=23 nM; p110α/β/δ/γ, IC50=2 nM/3 nM/3 nM/15 nM; mTOR, IC50=30 nM.

活性最高的DNA-PK抑制剂

PIK-75 : DNA-PK, IC50=2 nM.

最新的DNA-PK抑制剂

KU-0060648 : Dual inhibitor of DNA-PK and PI3Kα, PI3Kβ, PI3Kδ with IC50 of 8.6 nM and 4 nM, 0.5 nM, 0.1 nM respectively, less inhibition of PI3Kγ with IC50 of 0.59 μM.

经典的DNA-PK抑制剂

NU7026 : Potent DNA-PK inhibitor with IC50 of 0.23 μM, 60-fold selective for DNA-PK than PI3K and inactive against both ATM and ATR.

相关DNA-PK产品

CHIR-99021 (CT99021) ^New

CHIR-99021 (CT99021)是一种GSK-3α和GSK-3β抑制剂， IC50分别为10 nM and 6.7 nM。CHIR99201对CDKs没有交叉反应性，对GSK-3β的选择性是对CDKs选择性的350倍。CHIR-99021 可作为Wnt/β-catenin的激活剂并诱导自噬。

Dorsomorphin (Compound C) ^New

Dorsomorphin (Compound C, BML-275)是一种有效的，可逆选择性 AMPK 抑制剂，无细胞试验中 K_i 为 109 nM，对几个结构相关的激酶，包括ZAPK，SYK，PKCθ，PKA，和 JAK3没有显著的抑制作用。也会抑制 I型BMP receptors ALK2，ALK3和ALK6 的活性。Dorsomorphin被应用于促进特定细胞分化和诱导癌细胞自噬。

MHY1485 ^New

MHY1485是一种有效的，细胞渗透性 mTOR 激动剂，也能有效抑制 autophagy。

NU7026

NU7026 (LY293646)是一种有效的DNA-PK抑制剂，在无细胞试验中IC50为0.23 μM，作用于DNA-PK比作用于PI3K选择性高60倍，对ATM和ATR没有抑制活性。NU7026 可加强G2/M周期阻滞和凋亡。

KU-0060648

KU-0060648是DNA-PK和PI3Kα/β/δ双重抑制剂，IC50分别为8.6 nM和4 nM/0.5 nM/0.1 nM，对PI3Kγ抑制作用稍弱，IC50为0.59 μM。

Rapamycin (Sirolimus)

Rapamycin (Sirolimus, AY 22989, NSC-2260804)是一种特定的 mTOR 抑制剂，在 HEK293细胞中，IC50 为 ~0.1 nM。

MK-2206 2HCl

MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂，在无细胞试验中IC50分别为8 nM/12 nM/65 nM；对250种其他蛋白激酶没有抑制活性。MK-2206 2HCl 在肿瘤细胞中可诱导自噬和凋亡。Phase 2。

Features:MK-2206是第一个进入临床研究阶段的Akt小分子变构抑制剂。

CHIR-99021 (CT99021) HCl

CHIR-99021 (CT99021) HCl是CHIR-99021的盐酸盐, 是GSK-3α/β抑制剂，IC50为10 nM/6.7 nM。其对GSK-3的选择性比对Cdc2和ERK2高500倍以上。CHIR-99021 是一种有效的药理性的 Wnt/beta-catenin 信号通路的激活剂。CHIR-99021 可显著地拯救光诱导的 autophagy，并可增强 GR，RORα和自噬相关蛋白等表达。

LY294002

LY294002 (SF 1101, NSC 697286) 是首个合成的已知抑制PI3Kα/δ/β的小分子，在无细胞测定中IC50分别为 0.5 μM/0.57 μM/0.97 μM；在溶液中比在Wortmannin中稳定，也能够阻断自噬体的形成。它不仅能够结合class I PI3Ks和其他PI3K相关的激酶，还能够结合一些与PI3K家族无关的新型靶点。LY294002 可以抑制CK2，对应的IC50值为98 nM。LY294002 是一种非特异的 DNA-PKcs 抑制剂，并可激活自噬和凋亡。

Features:LY294002是选择性的, 可渗透细胞的特定PI3K抑制剂，可作用于酶的ATP结合位点，且抑制自体吞噬的螯合作用。

3-Methyladenine (3-MA)

3-Methyladenine (3-MA, NSC 66389) 是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，在 HeLa细胞中IC50分别为25 μM和60 μM；永久性抑制I型PI3K，但对III型PI3K的抑制是短暂的，也会阻断自噬体的形成。3-Methyladenine (3-MA)可成功应用于抑制线粒体自噬。现配现用（加热助溶）。

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NU7441 (KU-57788)

客户使用Selleck生产的NU7441 (KU-57788)发表文献147篇：

产品安全说明书

DNA-PK抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download NU7441 (KU-57788) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

技术支持

常见问题及建议解决方法

DNA-PK Signaling Pathway Map

DNA-PK Inhibitors with Unique Features

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