Staurosporine (AM-2282)

For research use only. Not for use in humans.

目录号:S1421 别名: Antibiotic AM-2282, STS 中文名称:星形孢菌素

Staurosporine (AM-2282) Chemical Structure

CAS No. 62996-74-1

Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。

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RMB 2186.89 现货
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RMB 12203.1 现货
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客户使用Selleck生产的Staurosporine (AM-2282)发表文献96篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。
靶点
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外研究

Staurosporine 也强抑制HeLa S3细胞,IC50为4 nM。[1]Staurosporine 也抑制多种其他蛋白激酶,包括PKA, PKG, 磷酸激酶, S6 激酶, 肌球蛋白轻链激酶(MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, 和Syk , IC50分别为 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, 和 16 nM。[3] Staurosporine (1 μM) 诱导 PC12细胞90%以上凋亡,这种作用可被过量表达的Bcl-2抑制,或者zVAD-fmk, cycloheximide (10 μM) 及actinomycin D (5 μM)处理也可抑制。相应地, Staurosporine处理,诱导细胞内游离钙水平 [Ca2+]i快速且持久的提升,线粒体活性氧(ROS)的积累,及 随后的线粒体功能障碍。[4]通过caspase-8激活和Bid 分裂,功能性caspase-3的表达可增强Staurosporine诱导MCF7细胞死亡。[5] 1 μM Staurosporine处理,只部分抑制IL-3刺激的Bcl2 磷酸化,而完全阻断PKC调节的 Bcl2磷酸化。[6] Staurosporine 诱导人类包皮成纤维细胞AG-1518凋亡,根据溶酶体组织蛋白酶D调节的 细胞色素 c释放和 caspase 激活。[7] 除了激活传统线粒体凋亡通路, Staurosporine触发一种新型内在凋亡通路, 依赖Apaf-1存在时对caspase-9的激活。[8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 MmrQSpVv[3Srb36gZZN{[Xl? M4HZO2NwdmOnboTyZZRqd25icnXxeYlz\WRiZn;yJIdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJINwdG:wIHPhdoNqdm:vYTDj[YxtKGyrbnWgTGNVOTF4LDDJR|UxRTZibl2u NXr4dHZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1N|c{PDVpPkG1OVM4OzR3PD;hQi=>
human PBMC NGDCeo5HfW6ldHnvckBie3OjeR?= NUHNe3ExOjRiaB?= NH7mc4lUfXCycnXzd4lwdiCxZjDJUFIheHKxZIXjeIlwdiCrbjDoeY1idiCSQl3DJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF0yPiCwTT6= MlP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3OEWwOFYoRjF6NUi1NFQ3RC:jPh?=
endothelial cells NGm0RXpHfW6ldHnvckBie3OjeR?= NEjtN|NG\m[nY4TpeoUh\G:|ZTDh[4FqdnO2IIDsZZNucW6xZ3XuJIFkfGm4YYTvdkBi[3Srdnn0fUB{fGmvdXzheIVlKGK7IIDoc5Jjd2xiZYP0[ZIhcW5iZX7kc5Rp\WyrYXygZ4VtdHNuIFXEOVAhRSB5LkWg{txONg>? NITr[W49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96N{C5NFk2Lz56N{C5NFk2RC:jPh?=
Sf9 insect cells MnHSSpVv[3Srb36gZZN{[Xl? M{DWNWlvcGmkaYTpc44hd2ZicILveIVqdiCtaX7hd4UhSyhzOkHtbZh1fXKnIH;mJHBMSyCjbIDoZUAnKGKndHGpJIV5eHKnc4Pl[EBqdiCVZkmgbY5{\WO2IHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDd|NkC1K|46QDd|NkC1QE9iRg>?
LoVo cells NXTHfWlrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NFrXdYFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIHPvcI9vKGOjbnPldkBk\WyuIHzpcoUhMEyxVn:gZ4VtdHNrIIXzbY5oKE2WVDDhd5NigSxiSVO1NEA:KDBwMECxJO69VS5? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7MUWwOUc,OTF3OUG1NFU9N2F-
umbilical vein endothelial cells NWfpO4JsSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NX\BW29ESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjD1cYJqdGmlYXygeoVqdiCnbnTveIhmdGmjbDDj[YxteyBqSGXWSWN{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByNDFOwG0v NGTh[GE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
ST-486 cells MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHL1dotqfCCueX3wbI9u[SClZXzsJIxqdmViKGPUMVQ5PiClZXzsd{khfXOrbnegUXRVKGG|c3H5MEBKSzVyIE2gNE4xODdizszNMi=> NWXFdGo6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
DLD-1 cells Mli0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlvtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhFVERvMTDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEmg{txONg>? NIj4W5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
HEK293 cells MX3GeY5kfGmxbjDhd5NigQ>? MlXxTY5pcWKrdHnvckBw\iCSS1OtZoV1[SCLSTDt[YRq[XSnZDDJUE05KHKnbHXhd4Uh\nKxbTDISWszQTNiY3XscJMh[nliRVzJV2EtKEmFNUCgQUAxNjB5NzFOwG0v NXjsSHEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK5OFE{OzFpPkGyPVQyOzNzPD;hQi=>
P19 cells M3rON2Z2dmO2aX;uJIF{e2G7 NYLLWmNNUW6qaXLpeIlwdiCxZjDQcIF1\WyndD3k[ZJqfmWmIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFIh|ryPLh?= M4DFVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells MVzGeY5kfGmxbjDhd5NigQ>? MUDJcohq[mm2aX;uJI9nKFCuYYTlcIV1NWSncnn2[YQh\3Kxd4ToJIZi[3SxcjDy[YNmeHSxcjDpckBROTliY3XscJMtKEmFNUCgQUAxNjByMjFOwG0v NGCwTlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells Ml\OSpVv[3Srb36gZZN{[Xl? MkXDTY5pcWKrdHnvckBw\iCScn;0[YlvKEurbnHz[UBCKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNFA1KM7:TT6= Mn3NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells M2jS[mZ2dmO2aX;uJIF{e2G7 NHHDc4xKdmirYnn0bY9vKG:oIFPhcINqfW1xY3HscY9lfWyrbj3k[ZBmdmSnboSgdJJwfGWrbjDrbY5ie2VidInw[UBKUSCrbjDQNVkh[2WubIOsJGlEPTBiPTCwMlAxPiEQvF2u NWPjO4lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells NFS1cZBHfW6ldHnvckBie3OjeR?= NH\uUXRKdmirYnn0bY9vKG:oIFP5Z4xqdi2mZYDlcoRmdnRia3nuZZNmKDFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xODhizszNMi=> M4PrXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells NYfxeopRTnWwY4Tpc44h[XO|YYm= NGfWV4pKdmirYnn0bY9vKG:oIG\hd4N2dGG{IHXu[I91cGWuaXHsJIdzd3e2aDDmZYN1d3JicnXj[ZB1d3JiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xOTRizszNMi=> NIjob4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
HEK293 cells MnrUSpVv[3Srb36gZZN{[Xl? M3vscWlvcGmkaYTpc44hd2ZiSVytPEBz\WynYYPlJIJ6KEiHS{K5N{Bk\WyuczDlfJBz\XO|aX7nJHBMSy2kZYThNkwhUUN3MDC9JFAvODd5IN88UU4> MoeyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NG\sOpBHfW6ldHnvckBie3OjeR?= NH7yXWVKdmirYnn0bY9vKG:oIFnud5VtcW5icnXj[ZB1d3Jia3nuZZNmKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNkDPxE1w NIfkW2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells NYe3codQTnWwY4Tpc44h[XO|YYm= MX;Jcohq[mm2aX;uJI9nKEOjc3XpckBscW6jc3WgNUBqdiCSMUmgZ4VtdHNuIFnDOVAhRSBzLkSg{txONg>? MmrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
FL5.12-Akt1 cells M3r0OWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnP0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDGUFUvOTJvQXv0NUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ7IN88UU4> NF;0[Yk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwN|YzPid-MU[0NFM3OjZ:L3G+
MiaPaCa-2 cells MmrpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NY\vS2RjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBOcWGSYVPhMVIh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5|NzFOwG0v MkLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEO2NlYoRjF4NECzOlI3RC:jPh?=
FL5.12-Akt1 cells NU\4fId1TnWwY4Tpc44h[XO|YYm= MW\BZ5Rqfmm2eTDh[4FqdnO2IFfTT|MhcW5iRly1MlEzNUGtdEGgZ4VtdHNuIFXDOVAhRSByLkS2JO69VS5? M{PWVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NECzOlI3Lz5zNkSwN|YzPjxxYU6=
MiaPaCa-2 cells MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2nEZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTXnhVIFE[S1{IHPlcIx{NCCLQ{WwJF0hOC5|NzFOwG0v MnrNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MUO3PFAoRjF4NEGzO|gxRC:jPh?=
FL5.12-Akt1 cells M3HKXmZ2dmO2aX;uJIF{e2G7 NUfPOVJqSWO2aY\peJkh[WejaX7zeEBIW0t|IIDoc5NxcG:{eXzheIlwdiCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= NYTycYV4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NVM4QDBpPkG2OFE{PzhyPD;hQi=>
Sf21 cells MnPlSpVv[3Srb36gZZN{[Xl? MYDJcohq[mm2aX;uJI9nKEqDS{Og[ZhxemW|c3XkJIlvKFOoMkGgZ4VtdHNuIFnDOVAhRSByLkCwOkDPxE1w MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB6OEC1PUc,OTdyOEiwOVk9N2F-
U937 cells NXTLR2tDS3m2b4TvfIlkcXS7IHHzd4F6 NG[5[YZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{NCCLQ{WwJF0hOiEQvF2u M4LTfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEi4NFY4Lz5zN{C4PFA3PzxxYU6=
Sf9 cells M4jYbGZ2dmO2aX;uJIF{e2G7 NUfaV2xEOSCvaX6= NVfNS2xqUW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAyKG2rbjDifUBGVEmVQTDpckBxemW|ZX7j[UBw\iBzIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlAxPSEQvF2u NEHsdIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells Mo[0SpVv[3Srb36gZZN{[Xl? NYnqTYdpOjBibXnudy=> NYP0XmY3UW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAzOCCvaX7zJIJ6KEWOSWPBJIlvKHC{ZYPlcoNmKG:oIEGgeY1wdC:OIFHUVEwhUUN3MDC9JFAvODF3IN88UU4> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
Sf9 cells M4PHOmZ2dmO2aX;uJIF{e2G7 MnHlNUBucW5? NGnadIdKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFEhdWmwIHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlA{PiEQvF2u NGHLOWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells NWLsWW5tTnWwY4Tpc44h[XO|YYm= NEiyRVMzOCCvaX7z MYDJcohq[mm2aX;uJI9nKGi3bXHuJGZ6diCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIFnfGW{IEKwJI1qdnNiYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOTBidX3vcE9NKEGWUDygTWM2OCB;IECuNFg{KM7:TT6= M4W4NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{G1PFU{Lz5zN{OxOVg2OzxxYU6=
Sf9 cells MUfGeY5kfGmxbjDhd5NigQ>? NFm4elQyKG2rbh?= NFzCbYVKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFEhdWmwIHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyMDD1cY9tN0xiQWTQMEBKSzVyIE2gNE4zPyEQvF2u NXS4UYhIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVU5PTNpPkG3N|E2QDV|PD;hQi=>
Sf9 cells NH\KV3dHfW6ldHnvckBie3OjeR?= MoryNlAhdWmwcx?= MmjKTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCyNEBucW6|IHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyMDD1cY9tN0xiQWTQMEBKSzVyIE2gNE42OSEQvF2u MoTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|MUW4OVMoRjF5M{G1PFU{RC:jPh?=
A549 cells MmP6R5l1d3SxeHnjbZR6KGG|c3H5 NHX1ZZc4OiCq MnLMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDt[ZRpd2RuIFfJOVAhRSByLkCwNlQh|ryPLh?= NXLmTm1IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0PFQ4PzVpPkG4OFg1Pzd3PD;hQi=>
MDA-MB-231 cells MX7DfZRwfG:6aXPpeJkh[XO|YYm= MoqwO|IhcA>? MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKG2ndHjv[EwhT0l3MDC9JFAvODB5MTFOwG0v NYjjRW5PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0PFQ4PzVpPkG4OFg1Pzd3PD;hQi=>
HT29 cells NGTFN3FEgXSxdH;4bYNqfHliYYPzZZk> NIjwTIg4OiCq NFSyTmdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXDJ7IHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJI1mfGixZDygS2k2OCB;IECuNFExQSEQvF2u NEjzXGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES4OFc4PSd-MUi0PFQ4PzV:L3G+
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MCF7 cells Ml\xSpVv[3Srb36gZZN{[Xl? NGjJZmVKdmirYnn0bY9vKG:oIGDLRUBqdiCqdX3hckBOS0Z5IHPlcIx{KGK7IHHydoF6NWKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFIh|ryPLh?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ3NkO2PUc,OTh4NU[zOlk9N2F-
Sf9 cells M3vPcWZ2dmO2aX;uJIF{e2G7 NWrHOGN6UW6qaXLpeIlwdiCxZjDoeY1idiCVeXug[ZhxemW|c3XkJIlvKFOoOTDj[YxteyxiSVO1NEA:KDBwMECzJO69VS5? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{M{e4OEc,OTh6MkO3PFQ9N2F-
Sf9 cells MmPmSpVv[3Srb36gZZN{[Xl? NHWzUXpKdmirYnn0bY9vKG:oIHj1cYFvKFqDUEewJIV5eHKnc4Pl[EBqdiCVZkmgZ4VtdHNuIFnDOVAhRSByLkC1N{DPxE1w MoDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6MkO3PFQoRjF6OEKzO|g1RC:jPh?=
insect cells NUPhO|VGTnWwY4Tpc44h[XO|YYm= NGDqV2xKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IGDpcVEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BjgSCKVGLGMEBKSzVyIE2gNE4xOSEQvF2u MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF5OUC3Okc,OTlzN{mwO|Y9N2F-
HT-29 cells NEnrdWJCeG:ydH;zbZMh[XO|YYm= MkTpNkBp MWXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEiWLUK5JINmdGy|IHHzd4V{e2WmIHHzJINp[W6pZTDpckBucXSxY3jvcoRzcWGuIITyZY5{dWWvYoLhcoUheG:2ZX70bYFtKGGodHXyJFIhcHK|IHL5JGpEOSC|dHHpcolv\yCkYYPl[EBndHWxcnXzZ4Vv[2ViYYPzZZktKEWGNUCgQUAxNjByMk[g{txONg>? MmXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MkKyNFYoRjF7NEKyNlA3RC:jPh?=
insect Sf21 cells NGjtSpBHfW6ldHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKEqDS{Og[ZhxemW|c3XkJIlvKGmwc3XjeEBU\jJzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[mmxdHnufYxifGWmIIP1ZpN1emG2ZTDwbI9{eGixconsZZRqd25uIFnDOVAhRSByLkCwOkDPxE1w NFzU[pY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSyO|IxOyd-MUm0NlczODN:L3G+
Jurkat cells Mo\KRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHqwTGRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDJUFIue3SrbYXsZZRm\CCqdX3hckBLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkC3NUDPxE1w NXHSeo54RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlczODNpPkG5OFI4OjB|PD;hQi=>
Jurkat cells M3faUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFjTfZBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDoeY1idiC{ZYP0bY5oKEq3cnvheEBk\WyuczygTWM2OCB;IECuN|EyKM7:TT6= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR{N{KwN{c,OTl2MkeyNFM9N2F-
HUE cells MUjGeY5kfGmxbjDhd5NigQ>? NFHCWGs6OCCvaX7z NV3FfpB1UW6qaXLpeIlwdiCxZjDWSWdHWjJiaX6gTHVGKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gWmVITi2rbnT1Z4VlKGG3dH;wbI9{eGixconsZZRqd25idILlZZRm\CCob4KgPVAhdWmwczDi[YZwemViVlXHSkBkcGGubHXu[4Uh[nliRVzJV2EtKEmFNUCgQUAxNjB5IN88UU4> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF5MEG2N{c,OjBzN{CxOlM9N2F-
Sf9 cells M1rSdGZ2dmO2aX;uJIF{e2G7 M1XaRlYxKG2rboO= NWXGeZZlUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDHV3Qu\nW|ZXSgUmlMKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZYZ1\XJiNkCgcYlveyCkeTDyZYRqd22ndILpZ{Bxem:2ZXnuJItqdmG|ZTDhd5NigSxiSVO1NEA:KDJwMzFOwG0v Mkn6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3OEC1OVIoRjJyNUiwOVUzRC:jPh?=
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Sf21 cells MnmxSpVv[3Srb36gZZN{[Xl? NELSbmc{OCC3TR?= MYXJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEmNSz3hcJBp[SCneIDy[ZN{\WRiaX6gbY5{\WO2IGPmNlEh[2WubIOgeZNqdmdicHjvd5Bpdy2XbHnnbJQuUWujcIDhRk1idHCqYTDhd{B{fWK|dILheIUh[XRiM{CgeW0tKEmFNUCgQUAxNjB|OTFOwG0v NVHob4M{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|Q4QTZpPkKxN|M1Pzl4PD;hQi=>
Sf21 cells MV7GeY5kfGmxbjDhd5NigQ>? Mon5N|AhfU1? NGrOZVJKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFnLT{1j\XSjIHX4dJJme3OnZDDpckBqdnOnY4SgV4YzOSClZXzsd{B2e2mwZzDwbI9{eGixLWXsbYdpfC2La3HwdIFDNWGucHjhJIF{KHO3YoP0doF1\SCjdDCzNEB2VSxiSVO1NEA:KDBwNEmg{txONg>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|NEe5Okc,OjF|M{S3PVY9N2F-
HeLa cells MVfDfZRwfG:6aXPpeJkh[XO|YYm= NH7NWpU1QCCq NIDtXm9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjByMECwOEDPxE1w MlfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|OEixPVEoRjJzM{i4NVkyRC:jPh?=
MCF7 cells NYXNeWxIS3m2b4TvfIlkcXS7IHHzd4F6 Mm\ZOFghcA>? M1\2b2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODVizszNMi=> NWLZVZlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPFgyQTFpPkKxN|g5OTlzPD;hQi=>
HT-29 cells NV;VU3B2TnWwY4Tpc44h[XO|YYm= NFrJS5czKGh? MYXF[oZm[3Rib36gcYl1d2Oqb37kdolidCCvZX3idoFv\SCyb4TlcpRq[WxiaX6gbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiMjDodpMhfXOrbnegTmMuOSC|dHHpcolv\yCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlAxOiEQvF2u MkWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2MkizO|UoRjJzNEK4N|c2RC:jPh?=
HeLa cells NHnaU3dEgXSxdH;4bYNqfHliYYPzZZk> NIfucWM1QCCq NVTOdWl3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMECwNFQh|ryPLh?= MnHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2OEi2OVUoRjJzNEi4OlU2RC:jPh?=
MCF7 cells MYfDfZRwfG:6aXPpeJkh[XO|YYm= NFnKN241QCCq Ml;xR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xPSEQvF2u MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR6OE[1OUc,OjF2OEi2OVU9N2F-
HT-29 cells MWrGeY5kfGmxbjDhd5NigQ>? NXjiU25HUW6qaXLpeIlwdiCxZjDtbZRw[2ixbnTybYFtKG2nbXLyZY5mKHCxdHXueIlidCCrbjDoeY1idiCKVD2yPUBk\WyuczD1d4lv\yCMQ{Gg[JlmKHO2YXnubY5oKGK7IH\seY9z\XOlZX7j[UBxdGG2ZTDy[YFl\XJiYYPzZZktKEmFNUCgQUAxNjByMkWg{txONg>? Ml7TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyPVMoRjJzNUGzNlk{RC:jPh?=
SF268 cells MY\DfZRwfG:6aXPpeJkh[XO|YYm= MYm0PEBp MlTLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvODR2IN88UU4> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K5OEc,OjF3MUOyPVQ9N2F-
CHOK1 cells M1npemN6fG:2b4jpZ4l1gSCjc4PhfS=> MUS0PEBp NHPoOWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEUE:NMTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwJF0hOC5zMzFOwG0v MnfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyPVQoRjJzNUGzNlk1RC:jPh?=
H460 cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= MXO0PEBp M4LzbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGg1PjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFMvPiEQvF2u NEDMeoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I6PCd-MkG1NVMzQTR:L3G+
HT-29 cells NX61UFdwS3m2b4TvfIlkcXS7IHHzd4F6 NX7rTHlGPDhiaB?= NInF[2ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwJF0hOy54IN88UU4> MkPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyPVQoRjJzNUGzNlk1RC:jPh?=
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HT-29 cells M3\OXWFxd3C2b4Ppd{Bie3OjeR?= M1TWVlIhcA>? MULJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEiWLUK5JINmdGy|IHHzd4V{e2WmIILl[JVkfGmxbjDv[kBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIIDveIVvfGmjbDDh[pRmeiB{IHjyd{BjgSC3c3nu[{BLSzFic4ThbY5qdmdiYomg[ox2d3Knc3PlcoNmKGOnbHytZoF{\WRiYYPzZZktKEWGNUCgQUAxNjByMk[g{txONg>? NIfEW|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm3N|ExOSd-MkG5O|MyODF:L3G+
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HepG2 cells Mnq5R5l1d3SxeHnjbZR6KGG|c3H5 MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{whTUN3MDC9JFIh|ryPLh?= NHvHTWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOxOlMyPyd-MkWzNVY{OTd:L3G+
Raji cells NXzRb5VTS3m2b4TvfIlkcXS7IHHzd4F6 MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzMEBGSzVyIE2gNkDPxE1w MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNzNkOxO{c,OjV|MU[zNVc9N2F-
BJ cells MofiR5l1d3SxeHnjbZR6KGG|c3H5 NUHJSWZHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkpiY3XscJMtKEWFNUCgQUAzKM7:TT6= MkDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MU[zNVcoRjJ3M{G2N|E4RC:jPh?=
HEK293 cells NEL4NodEgXSxdH;4bYNqfHliYYPzZZk> NE\zO3JEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOsJGVEPTBiPTCyJO69VS5? M1ryT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
A549 cells MkTWR5l1d3SxeHnjbZR6KGG|c3H5 M1;LUlQ5KGh? M3niV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODJizszNMi=> NF\YN4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
HeLa cells NV;xU4k1S3m2b4TvfIlkcXS7IHHzd4F6 NVfyNFJSPDhiaB?= M4PIS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODJ3IN88UU4> NEW3XJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
PC3 cells M3j2e2N6fG:2b4jpZ4l1gSCjc4PhfS=> M2jBZlQ5KGh? NFj6UFdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODNzIN88UU4> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NUmzOEc,OjV6MkW5N|Q9N2F-
MGC-803 cells NVfDeGw4TnWwY4Tpc44h[XO|YYm= Mn6wNlQhcA>? NFTZZYtKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gUWdENThyMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhXFKDUD3QR3IuTUyLU1GgZZN{[XluIFnDOVAhRSB6Lkm3JO69VS5? MmnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7MEC2OVYoRjJ4OUCwOlU3RC:jPh?=
Sf9 insect cells NVvnfHUxTnWwY4Tpc44h[XO|YYm= NWPDVZlpOTBibXnudy=> MnLjTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIFfTWEB1[WepZXSgdoVkd22kaX7hcpQh\nWubD3s[Y5ofGhiaIXtZY4hS0iNMTDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiU3[5JIlve2WldDDj[YxteyCjZoTldkAyOCCvaX7zJIJ6KGy3bXnu[ZNk\W62IFHEVE1IdG9iYYPzZZktKEmFNUCgQUAxNjByMUKg{txONg>? MoPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEmyNVEoRjJ5MEi5NlEyRC:jPh?=
Sf21 cells M{W0WWZ2dmO2aX;uJIF{e2G7 NITqOWQ3OCCvaX7z M1[ydGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSlHLNkBmgHC{ZYPz[YQhcW5iU3[yNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iVXzp[4h1NUODR1HHRWlGXESNRWnZWHZMTCCyaH;zdIhwenmuYYTpc44heHKnLXnuZ5Vj[XSnZDDi[YZwemVic4Xid5Rz[XSnIHHk[Il1cW:wIHHu[EBu\WG|dYLl[EBi\nSncjC2NEBucW6|IHL5JGxCVkOHIHTleIVkfGmxbjDt[ZRpd2RuIFnDOVAhRSByLkCwNkDPxE1w M4KxWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUO3N|U6Lz5{N{GzO|M2QTxxYU6=
Neuro2a cells M2\LR2N6fG:2b4jpZ4l1gSCjc4PhfS=> NFvVPGI4OiCq NV7GTm85S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVmW3cn:yZUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wNVE{KM7:TT6= NInSZ449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG1NlQzPyd-MkixOVI1Ojd:L3G+
MDA-MB-231 cells MV\GeY5kfGmxbjDhd5NigQ>? NH:yRmUyPiCq MYrJcohq[mm2aX;uJI9nKHKndHnuc4Jt[XO2b33hJJBzd3SnaX6gdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KEixZXPod5QhOzN|NEKgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6wOlYh|ryPLh?= NUTvPJJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0N|E{PDJpPkK4OFMyOzR{PD;hQi=>
Sf9 insect cells MYXGeY5kfGmxbjDhd5NigQ>? MlTDNlAhdWmwcx?= MoPBTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCQLYTldo1qdmGubImgS3NVNXSjZ3fl[EBGT0[UIFy4OVhTN1R5OUDNM2M4QTeVIITybZBt\SCvdYThcpQh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlvKFOoOTDpcpNm[3RiY3XscJMheHKnaX7jeYJifGWmIH\vdkAzOCCvaX7zJIZwdGyxd3XkJIJ6KGGmZHn0bY9vKG:oIGuzN3BeNUGWUDDt[YF{fXKnZDDh[pRmeiB{IHjyd{BjgSCoaXz0[ZIu[mmwZHnu[{Bu\XSqb3SsJGlEPTBiPTCwMlAxODJ7IN88UU4> M3vLVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEiyNVUyLz5{OES4NlE2OTxxYU6=
HEK293 cells NVexUpZ[S3m2b4TvfIlkcXS7IHHzd4F6 NVHWeJVJPzJiaB?= NUTvOlBmS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIhT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDDR|UxKD1iMD6wNFM2PCEQvF2u NYfWd2ZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2NlQ4ODFpPkK4OlI1PzBzPD;hQi=>
BT549 cells NHTwNY1EgXSxdH;4bYNqfHliYYPzZZk> NF20VmYzPCCq NG\BZ5JEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFU1QSClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA5KM7:TT6= NH;wWYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEewOVQ{Oid-Mki3NFU1OzJ:L3G+
MDA-MB-468 cells NGnlPYNEgXSxdH;4bYNqfHliYYPzZZk> NV72SXpnOjRiaB?= MkG2R5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTR4ODDj[YxteyCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjF3IN88UU4> NYPiV5h3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
MDA-MB-231 cells M4[3VmN6fG:2b4jpZ4l1gSCjc4PhfS=> NH3OWYYzPCCq MlHTR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ2IN88UU4> NWr2SVNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
Vero cells NYHoXHBtS3m2b4TvfIlkcXS7IHHzd4F6 NVnnXHM{OiCmYYnz MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjCyJIRigXNiYomgZ4VtdC22aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIGTDOVAhRSByLkCwNlQh|ryPLh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd4M{[0OUc,Ojh5NkO2OFU9N2F-
L929 cells NG\ZXZNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M173cFUh\GG7cx?= Mlu5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCOOUK5JINmdGy|IHHmeIVzKDViZHH5d{BjgSCZU2StNUBie3OjeTygTWM2OCB;IECuNkDPxE1w NGDwemY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
KB-3-1 cells NXPSZpZDSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXu1JIRigXN? MkDiRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNQj2zMVEh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= MkG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NVUoRjJ6OUW2PVE2RC:jPh?=
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FS4-LTM cells MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MojCNlQhcA>? MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE[VND3MWG0h[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
Vero cells NGjjbnFEgXSxdH;4bYNqfHliYYPzZZk> NETL[oIzKGSjeYO= Ml71R5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB{IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNFAzPCEQvF2u MmrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7OUOxNFYoRjJ6OUmzNVA3RC:jPh?=
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NIH/3T3 cells NYDPW3Y1S3m2b4TvfIlkcXS7IHHzd4F6 M1HOXFk3KGh? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDOTWgwO1R|IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEWFNUCgQUAxNjByODFOwG0v Mn;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
518A2 cells M4f1emN6fG:2b4jpZ4l1gSCjc4PhfS=> NYXW[5E6QTZiaB?= MU\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBGSzVyIE2gNE4xOyEQvF2u NXPWb5lKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxPVc4OzBpPkK5NVk4PzNyPD;hQi=>
A549 cells MkHKR5l1d3SxeHnjbZR6KGG|c3H5 M1nsfFk3KGh? NGXPdllEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEWFNUCgQUAxNjB2IN88UU4> M1rRZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
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HT-29 cells M{DtT2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{H1eVk3KGh? NFnu[GdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5zNTFOwG0v MofUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
PC3 cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= NFPiT3hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhUUN3MDC9JFAvODF5IN88UU4> NFHpVGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUO4PVEzOid-MkmzPFkyOjJ:L3G+
HCT116 cells MmDwR5l1d3SxeHnjbZR6KGG|c3H5 M3zrWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOC5yNUWg{txONg>? NFLaTHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUO4PVEzOid-MkmzPFkyOjJ:L3G+
insect cells MYDGeY5kfGmxbjDhd5NigQ>? MVKzNEBucW6| MojiTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCSRFfGVoJmfGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyC3c3nu[{BWdGmpaIStVI9tgSCJQWTbSWF[MDF8MUqxLX1vKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODByN{[g{txONg>? M3OzfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
Sf9 insect cells MmfOSpVv[3Srb36gZZN{[Xl? NYPEO4tbPjBibXnudy=> MmjQTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCNRGKg[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LVPBS2FISUmHVFTLSXl[XF[NRDDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDt[ZRpd2RuIFnDOVAhRSByLkCwNlEh|ryPLh?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
insect cells NYLx[plsTnWwY4Tpc44h[XO|YYm= MWCxNEBucW6| NGTMOG9KdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIGPyZ{BmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGXsbYdpfC2Sb3z5JGdCXFuHQWmoNVoyQjFrXX6gZZMhe3Wkc4TyZZRmKGGodHXyJFExKG2rboOgZpkhVEGQQ1WgcYV1cG:mLDDJR|UxKD1iMD6wNFUh|ryPLh?= NYH6U|VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
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insect cells NGCxU2pHfW6ldHnvckBie3OjeR?= NUHub3k2OTVibXnudy=> MknoTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFRFuxM2N6[2yrbjDCJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiVXzp[4h1NUOIRlvOTXZVWFKWUGDQV3FIUy2jbXnk[UBieyC|dXLzeJJifGViYX\0[ZIhOTVibXnud{BjgSCOQV7DSUBu\XSqb3SsJGlEPTBiPTCwMlAzPSEQvF2u MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
Sf9 insect cells MXHGeY5kfGmxbjDhd5NigQ>? NEnmUG4yPSCvaX7z NV\SVoxiUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDGcJQyKGW6cILld5Nm\CCrbjDT[lkhcW6|ZXP0JINmdGy|IIXzbY5oKFWuaXfoeE1VUyCyZYD0bYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiBzNTDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODN3IN88UU4> M4HKW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
Sf21 insect cells NVjGeGw6TnWwY4Tpc44h[XO|YYm= NUXYU2ZsOTVibXnudy=> MmTiTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCDdYLvdoEuSSCneIDy[ZN{\WRiaX6gV4YzOSCrboPlZ5Qh[2WubIOgeZNqdmdiVXzp[4h1NVKUUmPMUGUh[XNic4Xid5Rz[XSnIHHmeIVzKDF3IH3pcpMh[nliTFHOR2UhdWW2aH;kMEBKSzVyIE2gNE4xOzlizszNMi=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
insect cells MVvGeY5kfGmxbjDhd5NigQ>? NXPK[3FpPjBibXnudy=> NXfIXY1nUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDBT3QyKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhfXOrbnegR3JGSnSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkhVEGQQ1WgcYV1cG:mLDDJR|UxKD1iMD6wOVYh|ryPLh?= NF6ycIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg{Pid-Mkm1OFk5OzZ:L3G+
Sf9 insect cells NWjQN|ZmTnWwY4Tpc44h[XO|YYm= M{nDU|YxKG2rboO= MV\Jcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE[DSzDlfJBz\XO|ZXSgbY4hW2Z7IHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvVFugdIVxfGmmZTDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDt[ZRpd2RuIFnDOVAhRSByLkC2NkDPxE1w MnHkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells MlHRSpVv[3Srb36gZZN{[Xl? MV[2NEBucW6| NFmzVJJKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFHCUEBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGXsbYdpfC2WSzDw[ZB1cWSnIHHzJJN2[nO2cnH0[UBi\nSncjC2NEBucW6|IHL5JGxCVkOHIH3leIhw\CxiSVO1NEA:KDBwMkmg{txONg>? M4D4OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
insect cells NWDXfIpiTnWwY4Tpc44h[XO|YYm= Ml7tOlAhdWmwcx?= M4jkcWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iYz3NSXQh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvQ1HHRWdCUUWWRFvFXXlVXkuGIHHzJJN2[nO2cnH0[UBi\nSncjC2NEBucW6|IHL5JGxCVkOHIH3leIhw\CxiSVO1NEA:KDBwM{Gg{txONg>? NXfXbZRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
PC3 cells NE\0d|VEgXSxdH;4bYNqfHliYYPzZZk> M1zMNlczKGh? NYHWeYtRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjB|OTFOwG0v M2PUbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUW4NVE6Lz5{OUW1PFEyQTxxYU6=
Sf9 cells NYPi[5lpTnWwY4Tpc44h[XO|YYm= MWq0OUBucW6| NXHJW4RRUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDMZRmem2rbnHsJGhqey22YXfn[YQwVi22ZYLtbY5idCCJU2SteIFo\2WmIFXHSnIhMDZ4ODD0c{AyOjFyIILld4llfWW|KTDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiU3[5JINmdGy|IIXzbY5oKFCxbImtLGdtfSxiVInyLUBieyC|dXLzeJJifGVibXXhd5Vz\WRiYX\0[ZIhPDVibXnueZRmeyCkeTDLbY5ie2VvR3zvJIx2dWmwZYPj[Y51KGG|c3H5MEBKSzVyIE2gNE4xPDdizszNMi=> NFLDNHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwNlcyQSd-Mkm5NFI4OTl:L3G+
Sf9 cells NWjBcGRmTnWwY4Tpc44h[XO|YYm= NG\SRm81PSCvaX7z M1;le2lvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTj30[ZJucW6jbDDHV3QufGGpZ3XkJGhGWjJiKE[3PUB1dyBzMkW1JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgV4Y6KGOnbHzzJJV{cW6pIGDvcJkuMEeudTygWJlzMSCjczDzeYJ{fHKjdHWgcYVie3W{ZXSgZYZ1\XJiNEWgcYlvfXSnczDifUBMcW6jc3WtS4xwKGy3bXnu[ZNk\W62IHHzd4F6NCCLQ{WwJF0hOC5yNkC4JO69VS5? NFj3WnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwNlcyQSd-Mkm5NFI4OTl:L3G+
HT-29 cells MnPaSpVv[3Srb36gZZN{[Xl? NGnhTVc{KGh? M4LsbWlvcGmkaYTpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhOyCqcoOgZpkhUkNvMTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCyJO69VS5? NIL4cFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC1O|E2PSd-M{CwOVcyPTV:L3G+
Sf9 cells MlfzSpVv[3Srb36gZZN{[Xl? NIrrNldKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygSmxCTy22YXfn[YQhcHWvYX6g[pVtdC2uZX7neIghS0SNMUKgLFEhfG9iMUS5NEBz\XOrZIXld{kwVi22ZYLtbY5idCCKaYOteIFo\2WmIFP5Z2shMDFidH:gOVgxKHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDBWHAu\GWyZX7k[Y51KFWOaXfoeE01TS2EUEGgLHRpejN5L2TodlQ3MSC|dXLzeJJifGVicHXweIll\SCyaH;zdIhwenmuYYTpc44heHJuIFnDOVAhRSByLkC1N{DPxE1w NXHaWINVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOlc{PThpPkOwNFY4OzV6PD;hQi=>
Sf9 cells NGH2NIJHfW6ldHnvckBie3OjeR?= MXLJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTJiKEGgeI8hOTR7MDDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3MzFOwG0v M{PQflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyME[3N|U5Lz5|MEC2O|M2QDxxYU6=
Sf9 cells M2XIcGZ2dmO2aX;uJIF{e2G7 M1\xXWlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMzCoNUB1dyBzNUGyJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEW3JO69VS5? MmnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNkezOVgoRjNyME[3N|U5RC:jPh?=
Sf9 cells Mne3SpVv[3Srb36gZZN{[Xl? MmWyTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIF\MRWcufGGpZ3XkJIh2dWGwIH\1cIwudGWwZ4ToJGNFUzF|IDixJJRwKDF3MUKgdoV{cWS3ZYOpM24ufGW{bXnuZYwhUGm|LYTh[4dm\CCFeXPLJEgyKHSxIEW4NEBz\XOrZIXld{kh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iQWTQMYRmeGWwZHXueEBWVGmpaIStOGUuSlBzIDjUbJI{Py:WaIK0Okkhe3Wkc4TyZZRmKHCncITp[IUheGixc4Doc5J6dGG2aX;uJJBzNCCLQ{WwJF0hOC5yNUeg{txONg>? NVHXOXNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOlc{PThpPkOwNFY4OzV6PD;hQi=>
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Huh7.5 cells M{XuemN6fG:2b4jpZ4l1gSCjc4PhfS=> M3XoNFQ5KGh? NUPO[IF[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqNz61JINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlYh|ryPLh?= Mk\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzME[yPVEoRjNyMUC2NlkyRC:jPh?=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

15121855 24174874 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

22684244 25215174 15121855 19840952 15140398 19880522
体内研究 在局部贫血前,使用Staurosporine(0.1-10 ng)预处理局部贫血沙鼠和大鼠模型,可防止神经元损伤,这种作用存在剂量依赖性,说明CAl锥体细胞死亡涉及PKC。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
细胞实验:[4]
- 合并
  • Cell lines: PC12
  • Concentrations: 溶于DMSO,终浓度为1 μM
  • Incubation Time: ~32 小时
  • Method: 使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合,然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞,测定凋亡细胞百分数。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 短暂局部贫血的雄性Mongolian沙鼠或雄性 Wistar大鼠
  • Dosages: ~10 ng
  • Administration: 定向处理到海马双边CA1子域
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 466.53
化学式

C28H26N4O3

CAS号 62996-74-1
储存条件 粉状
溶于溶剂
别名 Antibiotic AM-2282, STS

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

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操作手册

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID