Staurosporine (AM-2282)

For research use only. Not for use in humans.

目录号:S1421 别名: Antibiotic AM-2282, STS 中文名称:星形孢菌素

Staurosporine (AM-2282) Chemical Structure

CAS No. 62996-74-1

Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。

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RMB 2186.89 现货
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RMB 12203.1 现货
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客户使用Selleck生产的Staurosporine (AM-2282)发表文献99篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。
靶点
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外研究

Staurosporine 也强抑制HeLa S3细胞,IC50为4 nM。[1]Staurosporine 也抑制多种其他蛋白激酶,包括PKA, PKG, 磷酸激酶, S6 激酶, 肌球蛋白轻链激酶(MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, 和Syk , IC50分别为 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, 和 16 nM。[3] Staurosporine (1 μM) 诱导 PC12细胞90%以上凋亡,这种作用可被过量表达的Bcl-2抑制,或者zVAD-fmk, cycloheximide (10 μM) 及actinomycin D (5 μM)处理也可抑制。相应地, Staurosporine处理,诱导细胞内游离钙水平 [Ca2+]i快速且持久的提升,线粒体活性氧(ROS)的积累,及 随后的线粒体功能障碍。[4]通过caspase-8激活和Bid 分裂,功能性caspase-3的表达可增强Staurosporine诱导MCF7细胞死亡。[5] 1 μM Staurosporine处理,只部分抑制IL-3刺激的Bcl2 磷酸化,而完全阻断PKC调节的 Bcl2磷酸化。[6] Staurosporine 诱导人类包皮成纤维细胞AG-1518凋亡,根据溶酶体组织蛋白酶D调节的 细胞色素 c释放和 caspase 激活。[7] 除了激活传统线粒体凋亡通路, Staurosporine触发一种新型内在凋亡通路, 依赖Apaf-1存在时对caspase-9的激活。[8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 NXvKVGJbTnWwY4Tpc44h[XO|YYm= MoHpR49v[2WwdILheIlwdiC{ZYH1bZJm\CCob4Kg[5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gZ49td25iY3HyZ4lvd22jIHPlcIwhdGmwZTDIR3QyOTZuIFnDOVA:PiCwTT6= NHH2blk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUWzO|M1PSd-MUW1N|c{PDV:L3G+
human PBMC NG\Gd3NHfW6ldHnvckBie3OjeR?= MmDCNlQhcA>? M37vWnN2eHC{ZYPzbY9vKG:oIFnMNkBxem:mdXP0bY9vKGmwIHj1cYFvKFCETVOgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVE3KG6PLh?= NHLBOok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4OVA1Pid-MUi1PFUxPDZ:L3G+
endothelial cells MnzlSpVv[3Srb36gZZN{[Xl? NVvZ[XNNTW[oZXP0bZZmKGSxc3WgZYdicW6|dDDwcIF{dWmwb3flckBi[3SrdnH0c5Ih[WO2aY\peJkhe3SrbYXsZZRm\CCkeTDwbI9z[m:uIHXzeIVzKGmwIHXu[I91cGWuaXHsJINmdGy|LDDFSFUxKD1iNz61JO69VS5? NE[0ZYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96N{C5NFk2Lz56N{C5NFk2RC:jPh?=
Sf9 insect cells MYDGeY5kfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKHC{b4TlbY4hc2mwYYPlJGMpOTpzbXn4eJVz\SCxZjDQT2Mh[WyyaHGgKkBj\XSjKTDlfJBz\XO|ZXSgbY4hW2Z7IHnud4VkfCClZXzsd{whUUN3MDC9JFAvODJizszNMi=> M4DLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{O2NFUoRjl6N{O2NFU9N2F-
LoVo cells NH:5bGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGOxbH;uJINidmOncjDj[YxtKGyrbnWgLGxwXm9iY3XscJMqKHW|aX7nJG1VXCCjc4PhfUwhUUN3MDC9JFAvODBzIN88UU4> NWHpWWdPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
umbilical vein endothelial cells M3PYUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NH7pSHpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIIXtZoltcWOjbDD2[YlvKGWwZH;0bIVtcWGuIHPlcIx{KCiKVW\FR5MqKHW|aX7nJG1VXCCjc4PhfUwhUUN3MDC9JFAvODB2IN88UU4> NV\Ee|kyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
ST-486 cells M13CcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHL1dotqfCCueX3wbI9u[SClZXzsJIxqdmViKGPUMVQ5PiClZXzsd{khfXOrbnegUXRVKGG|c3H5MEBKSzVyIE2gNE4xODdizszNMi=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7MUWwOUc,OTF3OUG1NFU9N2F-
DLD-1 cells MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NVf0VIdvSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubDDsbY5mKCiGTFStNUBk\WyuczmgeZNqdmdiTWTUJIF{e2G7LDDJR|UxKD1iMD6wNFkh|ryPLh?= M4\tdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNUmxOVA2Lz5zMUW5NVUxPTxxYU6=
HEK293 cells MlvXSpVv[3Srb36gZZN{[Xl? MYjJcohq[mm2aX;uJI9nKFCNQz3i[ZRiKEmLIH3l[IlifGWmIFnMMVghemWuZXHz[UBnem:vIFjFT|I6OyClZXzsd{BjgSCHTFnTRUwhUUN3MDC9JFAvODd5IN88UU4> NFT4c4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkm0NVM{OSd-MUK5OFE{OzF:L3G+
P19 cells NYTjdpJoTnWwY4Tpc44h[XO|YYm= NXr4cWF6UW6qaXLpeIlwdiCxZjDQcIF1\WyndD3k[ZJqfmWmIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFIh|ryPLh?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells NUfsWW9wTnWwY4Tpc44h[XO|YYm= NXX1dY1rUW6qaXLpeIlwdiCxZjDQcIF1\WyndD3k[ZJqfmWmIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFIh|ryPLh?= NYLy[o1ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells Mn\NSpVv[3Srb36gZZN{[Xl? NIS0cHRKdmirYnn0bY9vKG:oIGDyc5RmcW5iS3nuZZNmKEFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xODRizszNMi=> M3nlZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells NWD2NXJpTnWwY4Tpc44h[XO|YYm= NFr2bnVKdmirYnn0bY9vKG:oIFPhcINqfW1xY3HscY9lfWyrbj3k[ZBmdmSnboSgdJJwfGWrbjDrbY5ie2VidInw[UBKUSCrbjDQNVkh[2WubIOsJGlEPTBiPTCwMlAxPiEQvF2u M{C5OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells MUfGeY5kfGmxbjDhd5NigQ>? NVPUOoF5UW6qaXLpeIlwdiCxZjDDfYNtcW5vZHXw[Y5l\W62IHvpcoF{\SBzIHnuJHAyQSClZXzsd{whUUN3MDC9JFAvODB6IN88UU4> NUnLeVA6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells MUXGeY5kfGmxbjDhd5NigQ>? M3faU2lvcGmkaYTpc44hd2ZiVnHzZ5Vt[XJiZX7kc5Rp\WyrYXyg[5Jwf3SqIH\hZ5RweiC{ZXPldJRweiCrbjDQNVkh[2WubIOsJGlEPTBiPTCwMlAyPCEQvF2u MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
HEK293 cells MoXiSpVv[3Srb36gZZN{[Xl? M4ew[mlvcGmkaYTpc44hd2ZiSVytPEBz\WynYYPlJIJ6KEiHS{K5N{Bk\WyuczDlfJBz\XO|aX7nJHBMSy2kZYThNkwhUUN3MDC9JFAvODd5IN88UU4> NUmye2F5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells NYC0[ZpzTnWwY4Tpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKEmwc4XsbY4hemWlZYD0c5Ihc2mwYYPlJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMjFOwG0v MkSyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells MXnGeY5kfGmxbjDhd5NigQ>? NUTkT2xRUW6qaXLpeIlwdiCxZjDDZZNmcW5ia3nuZZNmKDFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNU41KM7:TT6= NHjvNWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
FL5.12-Akt1 cells M1W2O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NIXUOldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGZNPS5zMj3Bb5QyKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkmg{txONg>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
MiaPaCa-2 cells NGT0WJpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M{PKOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUYliWGGFYT2yJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOzdizszNMi=> MlezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEO2NlYoRjF4NECzOlI3RC:jPh?=
FL5.12-Akt1 cells MnLOSpVv[3Srb36gZZN{[Xl? MljDRYN1cX[rdImgZYdicW6|dDDHV2s{KGmwIF\MOU4yOi2Da4SxJINmdGy|LDDFR|UxKD1iMD60OkDPxE1w NYTpRoRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFM3OjZpPkG2OFA{PjJ4PD;hQi=>
MiaPaCa-2 cells NFLYfIJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NIDhTFNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3pZXBiS2FvMjDj[YxteyxiSVO1NEA:KDBwM{eg{txONg>? NWfVc|d4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NVM4QDBpPkG2OFE{PzhyPD;hQi=>
FL5.12-Akt1 cells NYrrcY51TnWwY4Tpc44h[XO|YYm= MVrBZ5Rqfmm2eTDh[4FqdnO2IFfTT|MheGixc4Doc5J6dGG2aX;uJIlvKE[ONT6xNk1Cc3RzIHPlcIx{NCCHQ{WwJF0hOC52NjFOwG0v MlvkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MUO3PFAoRjF4NEGzO|gxRC:jPh?=
Sf21 cells M1TteWZ2dmO2aX;uJIF{e2G7 Ml\ZTY5pcWKrdHnvckBw\iCMQVuzJIV5eHKnc4Pl[EBqdiCVZkKxJINmdGy|LDDJR|UxKD1iMD6wNFYh|ryPLh?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB6OEC1PUc,OTdyOEiwOVk9N2F-
U937 cells M4n6UmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkDZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{whUUN3MDC9JFIh|ryPLh?= NIexdoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{C4PFA3Pyd-MUewPFgxPjd:L3G+
Sf9 cells NVrVVI8xTnWwY4Tpc44h[XO|YYm= NUOyfVRlOSCvaX6= NIXFd|dKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFEhdWmwIHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFidX3vcE9NKEGWUDygTWM2OCB;IECuNFA2KM7:TT6= M{HlfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{G1PFU{Lz5zN{OxOVg2OzxxYU6=
Sf9 cells MXHGeY5kfGmxbjDhd5NigQ>? NVzGNoZnOjBibXnudy=> MlnyTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCyNEBucW6|IHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFidX3vcE9NKEGWUDygTWM2OCB;IECuNFE2KM7:TT6= NWLXd4VCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVU5PTNpPkG3N|E2QDV|PD;hQi=>
Sf9 cells MWHGeY5kfGmxbjDhd5NigQ>? NUL0cVFyOSCvaX6= MlHnTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCxJI1qdiCkeTDFUGlUSSCrbjDwdoV{\W6lZTDv[kAyOCC3bX;sM2whSVSSLDDJR|UxKD1iMD6wN|Yh|ryPLh?= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
Sf9 cells MX;GeY5kfGmxbjDhd5NigQ>? MlnoNlAhdWmwcx?= NYG2XZl5UW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAzOCCvaX7zJIJ6KEWOSWPBJIlvKHC{ZYPlcoNmKG:oIEGwJJVud2xxTDDBWHAtKEmFNUCgQUAxNjB6MzFOwG0v NFj1fWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
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Sf9 cells M2\qWWZ2dmO2aX;uJIF{e2G7 M1[yV|IxKG2rboO= MkPPTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCyNEBucW6|IHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyMDD1cY9tN0xiQWTQMEBKSzVyIE2gNE42OSEQvF2u MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
A549 cells M37QNGN6fG:2b4jpZ4l1gSCjc4PhfS=> MVy3NkBp MnnjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDt[ZRpd2RuIFfJOVAhRSByLkCwNlQh|ryPLh?= NH:4N2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES4OFc4PSd-MUi0PFQ4PzV:L3G+
MDA-MB-231 cells NGDHdVJEgXSxdH;4bYNqfHliYYPzZZk> NV7YUXQ4PzJiaB?= MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKG2ndHjv[EwhT0l3MDC9JFAvODB5MTFOwG0v M2nF[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEi0O|c2Lz5zOES4OFc4PTxxYU6=
HT29 cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= M160TFczKGh? NUXzNpVWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFR{OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBu\XSqb3SsJGdKPTBiPTCwMlAyODlizszNMi=> M1juNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEi0O|c2Lz5zOES4OFc4PTxxYU6=
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Sf9 cells M{LUbWZ2dmO2aX;uJIF{e2G7 M2qyRmlvcGmkaYTpc44hd2ZiaIXtZY4hYkGSN{Cg[ZhxemW|c3XkJIlvKFOoOTDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5? NGP0eG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
insect cells NV\ZVnRuTnWwY4Tpc44h[XO|YYm= NVSy[3RNUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDQbY0yKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMh[nliSGTSSkwhUUN3MDC9JFAvODFizszNMi=> NGH3V4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG3PVA4Pid-MUmxO|kxPzZ:L3G+
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insect Sf21 cells M{PFbWZ2dmO2aX;uJIF{e2G7 NXPJc3R1UW6qaXLpeIlwdiCxZjDKRWs{KGW6cILld5Nm\CCrbjDpcpNm[3RiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHLpc5RqdnmuYYTl[EB{fWK|dILheIUheGixc4Doc5J6dGG2aX;uMEBKSzVyIE2gNE4xODZizszNMi=> MkDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MkeyNFMoRjF7NEK3NlA{RC:jPh?=
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B16F10 cells M4DRPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MmHTOFghcA>? MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEJzNl[xNEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj64NkDPxE1w NG[2eWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWwN|M3PCd-MkK1NFM{PjR:L3G+
MCF7 cells NF7I[ZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYC0PEBp NYLCc25XSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzMlA4KM7:TT6= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVyM{O2OEc,OjJ3MEOzOlQ9N2F-
A431 cells MVLDfZRwfG:6aXPpeJkh[XO|YYm= M4jUOVI1KGh? MmXPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{Bi\nSncjCyOEBpenNidYPpcochSW6wZYjpckBX\U[LVFOvdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB5IN88UU4> NEfBZWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
MCF7 cells NFvmUJNEgXSxdH;4bYNqfHliYYPzZZk> NFHlToEzPCCq MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEK0JIhzeyC3c3nu[{BCdm6neHnuJHZmTkmWQz;wdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pIHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOThizszNMi=> NY\ve21ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFExPTFpPkKyOVQyODVzPD;hQi=>
BJ cells NVuzNoZ{S3m2b4TvfIlkcXS7IHHzd4F6 M17tdlczKGh? M3fEZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJLKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDDZYxk\WmwIFHNJIF{e2G7LDDJR|UxKD1iMD6wNFIh|ryPLh?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{MUC4NUc,OjJ7MkGwPFE9N2F-
CEM cells M{XxT2N6fG:2b4jpZ4l1gSCjc4PhfS=> MonvO|IhcA>? M3LKS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{Bi\nSncjC3NkBpenNiYomgR4Ft[2WrbjDBUUBie3OjeTygTWM2OCB;IECuNFI{KM7:TT6= M2XG[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUKxNFgyLz5{MkmyNVA5OTxxYU6=
MCF7 cells NXrQeZp1S3m2b4TvfIlkcXS7IHHzd4F6 NV3EbGUzPzJiaB?= NVPt[GE2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFAvODZ2IN88UU4> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{MUC4NUc,OjJ7MkGwPFE9N2F-
HeLa cells NUDTSG9LS3m2b4TvfIlkcXS7IHHzd4F6 M{HxcVczKGh? M3vMRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNidGOnaX6gRW0h[XO|YYmsJGlEPTBiPTCwMlE4PSEQvF2u NYLPcoFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
HepG2 cells NIDt[WhHfW6ldHnvckBie3OjeR?= MX2yOEBp MkLETY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDUVmFRNVCFUj3FUGlUSSCjc4PhfUwhUUN3MDC9JFQvOTRizszNMi=> NHXQV2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
SW1116 cells MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NVn6OJNPPDhiaB?= MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZMUGxOkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJI1mfGixZDygTWM2OCB;IESuPVUh|ryPLh?= NV60clVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVgxQTZpPkKzNFE5ODl4PD;hQi=>
HepG2 cells M2jHUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVe0PEBp MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgcYV1cG:mLDDJR|UxKD1iNj63N{DPxE1w MnLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
BGC823 cells MoWwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mn7mOFghcA>? NWTvUYRnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCvZYToc4QtKEmFNUCgQUA3Njh|IN88UU4> MonDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HeLa cells NYW4WIFHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIrwPHo1QCCq NXe3dnNlSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQhdWW2aH;kMEBKSzVyIE2gPU4yOiEQvF2u Mk[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
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HepG2 cells M17nS2Z2dmO2aX;uJIF{e2G7 MmPOOFghcA>? MULJcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iSHXwS|Ih[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGTSRXAuWEOULVXMTXNCNCCLQ{WwJF0hQC5|IN88UU4> MlvZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{N{m4OlQoRjJ|Mke5PFY1RC:jPh?=
SW1116 cells MkfHSpVv[3Srb36gZZN{[Xl? NF\FWmEzPCCq Mm\1TY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIGPXNVEyPiClZXzsd{Bi\nSncjCyOEBpenNiYomgWHJCWC2SQ2KtSWxKW0FiYYPzZZktKEmFNUCgQUA1NjF6IN88UU4> M3XhUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mki2O|YyLz5{NEK4Olc3OTxxYU6=
KE-97 cells NWLjSlQ6S3m2b4TvfIlkcXS7IHHzd4F6 NX;qV3I{PzJiaB?= MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLSU06PyClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdILlMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOTNizszNMi=> M3vz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K4Nlg{Lz5{NEOyPFI5OzxxYU6=
Jurkat cells M2P4XmN6fG:2b4jpZ4l1gSCjc4PhfS=> M3zBSlczKGh? NGfiR2tEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBLfXKtYYSgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRz\S2JbH:gcJVucW6nc3PlcpQh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkG0JO69VS5? NX;RVGU2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
HuH7 cells MVPDfZRwfG:6aXPpeJkh[XO|YYm= NUXDdmZ[PzJiaB?= M1jiV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjJ|IN88UU4> NW\1fHN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
BGC823 cells MVXDfZRwfG:6aXPpeJkh[XO|YYm= MVK3NkBp MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjN6IN88UU4> MnLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkiyPFMoRjJ2M{K4Nlg{RC:jPh?=
MCF7 cells M2DGR2N6fG:2b4jpZ4l1gSCjc4PhfS=> NW\oe|N6PzJiaB?= NYXIT4JtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwNUKg{txONg>? NUfidWhZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
NIH/3T3 cells MUnDfZRwfG:6aXPpeJkh[XO|YYm= M{f4Zlk3KGh? MmSwR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5{IN88UU4> NYnEcJFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
A2780 cells MlXLR5l1d3SxeHnjbZR6KGG|c3H5 MX65OkBp MkSyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NWXGcI9IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
HT-29 cells NITXeoNEgXSxdH;4bYNqfHliYYPzZZk> NH;vSno6PiCq M3nUemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjJizszNMi=> MmX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkG1NlEoRjJ2M{[xOVIyRC:jPh?=
8505C cells NILxXodEgXSxdH;4bYNqfHliYYPzZZk> M4XGO|k3KGh? NWDiNXZ2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hQDVyNVOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v NIn4fIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
518A2 cells NWPpXW1QS3m2b4TvfIlkcXS7IHHzd4F6 NYLGfGVNQTZiaB?= MmrLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gOVE5STJiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NWLjUpJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
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HEK293 cells Mo\aR5l1d3SxeHnjbZR6KGG|c3H5 MV63NkBp NE\pV4VEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSnclfsc{Bie3OjeTygTWM2OCB;IECuNFU3KM7:TT6= Mnm5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5NkOyOlIoRjJ2N{[zNlYzRC:jPh?=
HeLa cells MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXLGT295OjRidH:gOFghcA>? MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhOjRidH:gOFghcHK|IHL5JGNEUy16IHHzd4F6NCCLQ{WwJF0hOi55MjFOwG0v NWHJdVFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PVI5OTFpPkK0O|kzQDFzPD;hQi=>
A549 cells NXfxbld3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1TNSFI1KHSxIES4JIg> MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhOjRidH:gOFghcHK|IHL5JGNEUy16IHHzd4F6NCCLQ{WwJF0hOy5yNTFOwG0v MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd7MkixNUc,OjR5OUK4NVE9N2F-
HepG2 cells MlXUR5l1d3SxeHnjbZR6KGG|c3H5 MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{whTUN3MDC9JFIh|ryPLh?= M{TOTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
Raji cells NVrTT2RoS3m2b4TvfIlkcXS7IHHzd4F6 M3\IXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJicmliY3XscJMtKEWFNUCgQUAzKM7:TT6= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNzNkOxO{c,OjV|MU[zNVc9N2F-
BJ cells MnTVR5l1d3SxeHnjbZR6KGG|c3H5 NHnIW3NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDUiClZXzsd{whTUN3MDC9JFIh|ryPLh?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNzNkOxO{c,OjV|MU[zNVc9N2F-
HEK293 cells M2TKb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NVW5RmdPS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBGSzVyIE2gNkDPxE1w NWLKWWY1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
A549 cells NGPoUJNEgXSxdH;4bYNqfHliYYPzZZk> M1[4dlQ5KGh? NF;v[5BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{IN88UU4> MmHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
HeLa cells MYXDfZRwfG:6aXPpeJkh[XO|YYm= NHvZcpU1QCCq MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlAzPSEQvF2u MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NUmzOEc,OjV6MkW5N|Q9N2F-
PC3 cells Ml7ZR5l1d3SxeHnjbZR6KGG|c3H5 NITqb4k1QCCq NXHEN5VES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB|MTFOwG0v NGj4e249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
MGC-803 cells M4\wZ2Z2dmO2aX;uJIF{e2G7 NV3YOphEOjRiaB?= NUHqdZFOUW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJG1ISy16MEOgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFSUQWCtVGNTNUWOSWPBJIF{e2G7LDDJR|UxKD1iOD65O{DPxE1w M1POc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUCwOlU3Lz5{NkmwNFY2PjxxYU6=
Sf9 insect cells MmnZSpVv[3Srb36gZZN{[Xl? NGX3cWIyOCCvaX7z NFjHUnpKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygS3NVKHSjZ3fl[EBz\WOxbXLpcoFvfCCodXzsMYxmdme2aDDoeY1idiCFSFuxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDT[lkhcW6|ZXP0JINmdGy|IHHmeIVzKDFyIH3pcpMh[nlibIXtbY5me2OnboSgRWRRNUeubzDhd5NigSxiSVO1NEA:KDBwMECxNkDPxE1w M3LyWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEi5NlEyLz5{N{C4PVIyOTxxYU6=
Sf21 cells MVHGeY5kfGmxbjDhd5NigQ>? MUm2NEBucW6| MV7Jcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEqDS{Kg[ZhxemW|c3XkJIlvKFOoMkGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFWuaXfoeE1ESUeDR1HJSXRFU0W\WWTWT2QheGixc4Doc5J6dGG2aX;uJJBz\S2rbnP1ZoF1\WRiYnXmc5JmKHO3YoP0doF1\SCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiNkCgcYlveyCkeTDMRW5ETSCmZYTlZ5Rqd25ibXX0bI9lNCCLQ{WwJF0hOC5yMEKg{txONg>? MkTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzM{ezOVkoRjJ5MUO3N|U6RC:jPh?=
Neuro2a cells NGW1OVREgXSxdH;4bYNqfHliYYPzZZk> NYPZc2s4PzJiaB?= MkGxR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUoV2em9{YTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMUGzJO69VS5? NEjISGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG1NlQzPyd-MkixOVI1Ojd:L3G+
MDA-MB-231 cells MnzxSpVv[3Srb36gZZN{[Xl? NIj1WVUyPiCq NYfac2dwUW6qaXLpeIlwdiCxZjDy[ZRqdm:kbHHzeI9u[SCycn;0[YlvKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDIc4VkcHO2IEOzN|QzKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODZ4IN88UU4> M1XOWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEOxN|QzLz5{OESzNVM1OjxxYU6=
Sf9 insect cells MVrGeY5kfGmxbjDhd5NigQ>? MUWyNEBucW6| NUP0ZVFNUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDOMZRmem2rbnHscJkhT1OWLYTh[4dm\CCHR1\SJGw5PTiUL2S3PVBON0N5OUfTJJRzcXCuZTDteZRidnRiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwIGPmPUBqdnOnY4SgZ4VtdHNicILlbY5kfWKjdHXkJIZweiB{MDDtbY5{KG[xbHzve4VlKGK7IHHk[Il1cW:wIH;mJHs{O1CfLVHUVEBu\WG|dYLl[EBi\nSncjCyJIhzeyCkeTDmbYx1\XJvYnnu[Ilv\yCvZYToc4QtKEmFNUCgQUAxNjByMEK5JO69VS5? NGHVSoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES4NlE2OSd-Mki0PFIyPTF:L3G+
HEK293 cells MXXDfZRwfG:6aXPpeJkh[XO|YYm= M1jLOlczKGh? MkPvR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKgS4xwKGy3bXnu[ZNk\W62IHHzd4F6NCCFQ{WwJF0hOC5yMEO1OEDPxE1w NH76Vlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[yOFcxOSd-Mki2NlQ4ODF:L3G+
BT549 cells MkTuR5l1d3SxeHnjbZR6KGG|c3H5 MWeyOEBp Mmr0R5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wPEDPxE1w NYnRUZV1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
MDA-MB-468 cells NYHwXYdbS3m2b4TvfIlkcXS7IHHzd4F6 MYeyOEBp NFTl[lJEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMUWg{txONg>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDdyNUSzNkc,Ojh5MEW0N|I9N2F-
MDA-MB-231 cells NX3WcmdZS3m2b4TvfIlkcXS7IHHzd4F6 MXyyOEBp MX;DfZRwfG:6aXOgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOjRizszNMi=> M1fMblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{C1OFMzLz5{OEewOVQ{OjxxYU6=
Vero cells MXXDfZRwfG:6aXPpeJkh[XO|YYm= MUOyJIRigXN? MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjCyJIRigXNiYomgZ4VtdC22aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIGTDOVAhRSByLkCwNlQh|ryPLh?= NVe4RpU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3OlM3PDVpPkK4O|Y{PjR3PD;hQi=>
L929 cells NGXzPHNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVi1JIRigXN? MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEx7MkmgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxKD1iMD6yJO69VS5? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
KB-3-1 cells NEXoSFNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHPxS|Y2KGSjeYO= Ml7uRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNQj2zMVEh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= NFi1Snc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
MCF7 cells Mn;0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M{jKOVUh\GG7cx?= M3\VSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB3IHThfZMh[nliV2PUMVEh[XO|YYmsJGlEPTBiPTCxMlgh|ryPLh?= NHnGcZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
FS4-LTM cells MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MXOyOEBp NGLxRWhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF\TOE1NXE1iY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwJF0hOi56IN88UU4> M3HvfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVE2Lz5{OEm1OlkyPTxxYU6=
Vero cells NXqwXIdCS3m2b4TvfIlkcXS7IHHzd4F6 MYCyJIRigXN? MnHyR5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB{IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNFAzPCEQvF2u NH62NlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm5N|ExPid-Mki5PVMyODZ:L3G+
HepG2 cells MWrDfZRwfG:6aXPpeJkh[XO|YYm= NHrrcWcyODBidV2= M2TmcFMh\GG7cx?= M3Xme2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA{KGSjeYOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMEG4JO69VS5? NEDhZYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm5N|ExPid-Mki5PVMyODZ:L3G+
NIH/3T3 cells NYHBRYF3S3m2b4TvfIlkcXS7IHHzd4F6 NYOyb4VPQTZiaB?= MU\DfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDOTWgwO1R|IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEWFNUCgQUAxNjByODFOwG0v NYSweppzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxPVc4OzBpPkK5NVk4PzNyPD;hQi=>
518A2 cells NGG3UFhEgXSxdH;4bYNqfHliYYPzZZk> NFe2Ulg6PiCq MlXVR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gOVE5STJiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhTUN3MDC9JFAvODNizszNMi=> M1W0NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
A549 cells MXjDfZRwfG:6aXPpeJkh[XO|YYm= MUS5OkBp MnznR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBGSzVyIE2gNE4xPCEQvF2u M4DoWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
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A2780 cells M1TpdGN6fG:2b4jpZ4l1gSCjc4PhfS=> NWnoSpR7QTZiaB?= NV\DfFRCS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiRVO1NEA:KDBwMUKg{txONg>? Mm\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
HT-29 cells M1nUOmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFX0NlA6PiCq MknSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhTUN3MDC9JFAvOTVizszNMi=> MnLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
PC3 cells MWPDfZRwfG:6aXPpeJkh[XO|YYm= M1LIfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBKSzVyIE2gNE4xOTdizszNMi=> NYHkbmxKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzPFkyOjJpPkK5N|g6OTJ{PD;hQi=>
HCT116 cells NXWxV|ROS3m2b4TvfIlkcXS7IHHzd4F6 M1;ZfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOC5yNUWg{txONg>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNkc,Ojl|OEmxNlI9N2F-
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insect cells MnHISpVv[3Srb36gZZN{[Xl? NHvoOHEyPSCvaX7z NH3ubYVKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFPET|EwS3mlbHnuJGIh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvQ1\GT25KXlSSUmTQVHBUWUeNLXHtbYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiBzNTDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODJ3IN88UU4> M1GyR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
Sf9 insect cells Mmn3SpVv[3Srb36gZZN{[Xl? MnXZNVUhdWmwcx?= NInUfmpKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIF\seFEh\XiycnXzd4VlKGmwIGPmPUBqdnOnY4SgZ4VtdHNidYPpcochXWyrZ3j0MXRMKHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDF3IH3pcpMh[nliTFHOR2UhdWW2aH;kMEBKSzVyIE2gNE4xOzVizszNMi=> MnjqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
Sf21 insect cells NIHScoNHfW6ldHnvckBie3OjeR?= NWfDd2F3OTVibXnudy=> MWrJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEG3cn;yZU1CKGW6cILld5Nm\CCrbjDT[lIyKGmwc3XjeEBk\WyuczD1d4lv\yCXbHnnbJQuWlKUU1zMSUBieyC|dXLzeJJifGViYX\0[ZIhOTVibXnud{BjgSCOQV7DSUBu\XSqb3SsJGlEPTBiPTCwMlA{QSEQvF2u MoTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells M{npW2Z2dmO2aX;uJIF{e2G7 NXnNfnRDPjBibXnudy=> MnX0TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCDS2SxJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiQ2LFRpRq\GViYYOgd5Vje3S{YYTlJIFnfGW{IE[wJI1qdnNiYomgUGFPS0VibXX0bI9lNCCLQ{WwJF0hOC5yNU[g{txONg>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
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insect cells NX3ReVBpTnWwY4Tpc44h[XO|YYm= MoL2OlAhdWmwcx?= NXLYOHBSUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDBRmwh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvVFugdIVxfGmmZTDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDt[ZRpd2RuIFnDOVAhRSByLkK5JO69VS5? MmXZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells M4T6SGZ2dmO2aX;uJIF{e2G7 NEPoVI83OCCvaX7z M2fyZmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iYz3NSXQh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvQ1HHRWdCUUWWRFvFXXlVXkuGIHHzJJN2[nO2cnH0[UBi\nSncjC2NEBucW6|IHL5JGxCVkOHIH3leIhw\CxiSVO1NEA:KDBwM{Gg{txONg>? MojiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
PC3 cells NXjsS4x3S3m2b4TvfIlkcXS7IHHzd4F6 M3fRdVczKGh? M1:2RmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE4xOzlizszNMi=> NX7YOplbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OVgyOTlpPkK5OVU5OTF7PD;hQi=>
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Sf9 cells M3njbGZ2dmO2aX;uJIF{e2G7 MnjNOFUhdWmwcx?= M{DPXmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTj30[ZJucW6jbDDHV3QufGGpZ3XkJGhGWjJiKE[3PUB1dyBzMkW1JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgV4Y6KGOnbHzzJJV{cW6pIGDvcJkuMEeudTygWJlzMSCjczDzeYJ{fHKjdHWgcYVie3W{ZXSgZYZ1\XJiNEWgcYlvfXSnczDifUBMcW6jc3WtS4xwKGy3bXnu[ZNk\W62IHHzd4F6NCCLQ{WwJF0hOC5yNkC4JO69VS5? M4jBOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUCyO|E6Lz5{OUmwNlcyQTxxYU6=
HT-29 cells NHnDcllHfW6ldHnvckBie3OjeR?= MYGzJIg> MVzJcohq[mm2aX;uJI9nKG2rdH;jbI9v\HKrYXygcYVu[nKjbnWgdI91\W62aXHsJIlvKGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFMhcHK|IHL5JGpENTFic4ThbY5qdmdiYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMjFOwG0v NIfXNFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC1O|E2PSd-M{CwOVcyPTV:L3G+
Sf9 cells NXSwbJp{TnWwY4Tpc44h[XO|YYm= MWXJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTJiKEGgeI8hOTR7MDDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3MzFOwG0v NXzUb45qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOlc{PThpPkOwNFY4OzV6PD;hQi=>
Sf9 cells Mn7hSpVv[3Srb36gZZN{[Xl? MVvJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTJiKEGgeI8hOTR7MDDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3MzFOwG0v NXXQeJU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOlc{PThpPkOwNFY4OzV6PD;hQi=>
Sf9 cells NHKxfmJHfW6ldHnvckBie3OjeR?= MkTQTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIF\MRWcufGGpZ3XkJIh2dWGwIH\1cIwudGWwZ4ToJGNFUzF|IDixJJRwKDF3MUKgdoV{cWS3ZYOpM24ufGW{bXnuZYwhUGm|LYTh[4dm\CCFeXPLJEgyKHSxIEW4NEBz\XOrZIXld{kh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iQWTQMYRmeGWwZHXueEBWVGmpaIStOGUuSlBzIDjUbJI{Py:WaIK0Okkhe3Wkc4TyZZRmKHCncITp[IUheGixc4Doc5J6dGG2aX;uJJBzNCCLQ{WwJF0hOC5yNUeg{txONg>? NHzTUIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC2O|M2QCd-M{CwOlc{PTh:L3G+
Sf9 cells M2TNbmZ2dmO2aX;uJIF{e2G7 M2m5RWlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMzCoNUB1dyBzNUGyJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEW3JO69VS5? M2W2O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyME[3N|U5Lz5|MEC2O|M2QDxxYU6=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

15121855 24174874 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

22684244 25215174 15121855 19840952 15140398 19880522
体内研究 在局部贫血前,使用Staurosporine(0.1-10 ng)预处理局部贫血沙鼠和大鼠模型,可防止神经元损伤,这种作用存在剂量依赖性,说明CAl锥体细胞死亡涉及PKC。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
细胞实验:[4]
- 合并
  • Cell lines: PC12
  • Concentrations: 溶于DMSO,终浓度为1 μM
  • Incubation Time: ~32 小时
  • Method: 使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合,然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞,测定凋亡细胞百分数。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 短暂局部贫血的雄性Mongolian沙鼠或雄性 Wistar大鼠
  • Dosages: ~10 ng
  • Administration: 定向处理到海马双边CA1子域
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 466.53
化学式

C28H26N4O3

CAS号 62996-74-1
储存条件 粉状
溶于溶剂
别名 Antibiotic AM-2282, STS

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID