Staurosporine

For research use only. Not for use in humans.

目录号:S1421 别名: CGP 41251 中文名称:星形孢菌素

Staurosporine Chemical Structure

CAS No. 62996-74-1

Staurosporine (CGP 41251) 是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。

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RMB 12203.1 现货
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客户使用Selleck生产的Staurosporine发表文献88篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Staurosporine (CGP 41251) 是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。
靶点
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外研究

Staurosporine 也强抑制HeLa S3细胞,IC50为4 nM。[1]Staurosporine 也抑制多种其他蛋白激酶,包括PKA, PKG, 磷酸激酶, S6 激酶, 肌球蛋白轻链激酶(MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, 和Syk , IC50分别为 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, 和 16 nM。[3] Staurosporine (1 μM) 诱导 PC12细胞90%以上凋亡,这种作用可被过量表达的Bcl-2抑制,或者zVAD-fmk, cycloheximide (10 μM) 及actinomycin D (5 μM)处理也可抑制。相应地, Staurosporine处理,诱导细胞内游离钙水平 [Ca2+]i快速且持久的提升,线粒体活性氧(ROS)的积累,及 随后的线粒体功能障碍。[4]通过caspase-8激活和Bid 分裂,功能性caspase-3的表达可增强Staurosporine诱导MCF7细胞死亡。[5] 1 μM Staurosporine处理,只部分抑制IL-3刺激的Bcl2 磷酸化,而完全阻断PKC调节的 Bcl2磷酸化。[6] Staurosporine 诱导人类包皮成纤维细胞AG-1518凋亡,根据溶酶体组织蛋白酶D调节的 细胞色素 c释放和 caspase 激活。[7] 除了激活传统线粒体凋亡通路, Staurosporine触发一种新型内在凋亡通路, 依赖Apaf-1存在时对caspase-9的激活。[8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 NFnwWpBHfW6ldHnvckBie3OjeR?= MoryR49v[2WwdILheIlwdiC{ZYH1bZJm\CCob4Kg[5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gZ49td25iY3HyZ4lvd22jIHPlcIwhdGmwZTDIR3QyOTZuIFnDOVA:PiCwTT6= Ml;BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3M{ezOFUoRjF3NUO3N|Q2RC:jPh?=
human PBMC MmmwSpVv[3Srb36gZZN{[Xl? NVu1fYl5OjRiaB?= NWr5bXZ2W3WycILld5Nqd25ib3[gTWwzKHC{b3T1Z5Rqd25iaX6gbJVu[W5iUFLNR{Bi\nSncjCyOEBpenNiYomgSWxKW0FuIFnDOVA:OTZibl2u NHe4Wnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4OVA1Pid-MUi1PFUxPDZ:L3G+
endothelial cells MX\GeY5kfGmxbjDhd5NigQ>? NVPvRpV4TW[oZXP0bZZmKGSxc3WgZYdicW6|dDDwcIF{dWmwb3flckBi[3SrdnH0c5Ih[WO2aY\peJkhe3SrbYXsZZRm\CCkeTDwbI9z[m:uIHXzeIVzKGmwIHXu[I91cGWuaXHsJINmdGy|LDDFSFUxKD1iNz61JO69VS5? NF3x[mU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96N{C5NFk2Lz56N{C5NFk2RC:jPh?=
Sf9 insect cells MljtSpVv[3Srb36gZZN{[Xl? M1vwXWlvcGmkaYTpc44hd2ZicILveIVqdiCtaX7hd4UhSyhzOkHtbZh1fXKnIH;mJHBMSyCjbIDoZUAnKGKndHGpJIV5eHKnc4Pl[EBqdiCVZkmgbY5{\WO2IHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v NGC1bYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97OEezOlA2Lz57OEezOlA2RC:jPh?=
LoVo cells MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1vSRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY3;sc44h[2GwY3XyJINmdGxibHnu[UApVG:YbzDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEGg{txONg>? Mn\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3OUG1NFUoRjFzNUmxOVA2RC:jPh?=
umbilical vein endothelial cells NUHRTll2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4n0XGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5idX3ibYxq[2GuII\lbY4h\W6mb4To[Yxq[WxiY3XscJMhMEiXVlXDd{khfXOrbnegUXRVKGG|c3H5MEBKSzVyIE2gNE4xODRizszNMi=> NEftOGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
ST-486 cells NHT2Z3JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlXnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDieZJscXRibIntdIhwdWFiY3XscEBtcW6nIDjTWE01QDZiY3XscJMqKHW|aX7nJG1VXCCjc4PhfUwhUUN3MDC9JFAvODB5IN88UU4> NVGxWXE{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
DLD-1 cells NYTpT5RESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlP3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhFVERvMTDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEmg{txONg>? NWDwc|ZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
HEK293 cells MYXGeY5kfGmxbjDhd5NigQ>? MVjJcohq[mm2aX;uJI9nKFCNQz3i[ZRiKEmLIH3l[IlifGWmIFnMMVghemWuZXHz[UBnem:vIFjFT|I6OyClZXzsd{BjgSCHTFnTRUwhUUN3MDC9JFAvODd5IN88UU4> MkjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NEGzN|EoRjF{OUSxN|MyRC:jPh?=
P19 cells MX7GeY5kfGmxbjDhd5NigQ>? MYPJcohq[mm2aX;uJI9nKFCuYYTlcIV1NWSncnn2[YQh\3Kxd4ToJIZi[3SxcjDy[YNmeHSxcjDpckBROTliY3XscJMtKEmFNUCgQUAxNjByMjFOwG0v NGHQTJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells MWDGeY5kfGmxbjDhd5NigQ>? M{T4OGlvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells MlPjSpVv[3Srb36gZZN{[Xl? NFzNTo9KdmirYnn0bY9vKG:oIGDyc5RmcW5iS3nuZZNmKEFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xODRizszNMi=> M3WySFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells NEe3VllHfW6ldHnvckBie3OjeR?= M1vFcmlvcGmkaYTpc44hd2ZiQ3HsZ4l2dS:lYXztc4R2dGmwLXTldIVv\GWwdDDwdo91\WmwIHvpcoF{\SC2eYDlJGlKKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNFA3KM7:TT6= NIW3dnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells MkSxSpVv[3Srb36gZZN{[Xl? M{\aXmlvcGmkaYTpc44hd2ZiQ4njcIlvNWSncHXu[IVvfCCtaX7hd4UhOSCrbjDQNVkh[2WubIOsJGlEPTBiPTCwMlAxQCEQvF2u NWO2eo1jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells NU\LfGwzTnWwY4Tpc44h[XO|YYm= NELucJJKdmirYnn0bY9vKG:oIG\hd4N2dGG{IHXu[I91cGWuaXHsJIdzd3e2aDDmZYN1d3JicnXj[ZB1d3JiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xOTRizszNMi=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
HEK293 cells MoTDSpVv[3Srb36gZZN{[Xl? MkC4TY5pcWKrdHnvckBw\iCLTD24JJJmdGWjc3WgZpkhUEWNMkmzJINmdGy|IHX4dJJme3OrbnegVGtENWKndHGyMEBKSzVyIE2gNE4xPzdizszNMi=> M3\HRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells NVr1[IFQTnWwY4Tpc44h[XO|YYm= NGrjOXRKdmirYnn0bY9vKG:oIFnud5VtcW5icnXj[ZB1d3Jia3nuZZNmKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNkDPxE1w M1PLTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells NEnsRYpHfW6ldHnvckBie3OjeR?= MXzJcohq[mm2aX;uJI9nKEOjc3XpckBscW6jc3WgNUBqdiCSMUmgZ4VtdHNuIFnDOVAhRSBzLkSg{txONg>? M4SwWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
FL5.12-Akt1 cells MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF\MOU4yOi2Da4SxJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOjlizszNMi=> MoXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEO2NlYoRjF4NECzOlI3RC:jPh?=
MiaPaCa-2 cells NXu2Wo1[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3pZXBiS2FvMjDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlM4KM7:TT6= MnzvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEO2NlYoRjF4NECzOlI3RC:jPh?=
FL5.12-Akt1 cells NVf1UIZwTnWwY4Tpc44h[XO|YYm= M{nQZWFkfGm4aYT5JIFo[Wmwc4SgS3NMOyCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
MiaPaCa-2 cells NXGy[VlxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2rYWDhR4EuOiClZXzsd{whUUN3MDC9JFAvOzdizszNMi=> NH[4Tmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSxN|c5OCd-MU[0NVM4QDB:L3G+
FL5.12-Akt1 cells MlnQSpVv[3Srb36gZZN{[Xl? NYfrZ3VPSWO2aY\peJkh[WejaX7zeEBIW0t|IIDoc5NxcG:{eXzheIlwdiCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= MoTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MUO3PFAoRjF4NEGzO|gxRC:jPh?=
Sf21 cells NUHIXHVvTnWwY4Tpc44h[XO|YYm= NFn4N2FKdmirYnn0bY9vKG:oIFrBT|Mh\XiycnXzd4VlKGmwIGPmNlEh[2WubIOsJGlEPTBiPTCwMlAxPiEQvF2u M1XJXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEi4NFU6Lz5zN{C4PFA2QTxxYU6=
U937 cells NIDRdWREgXSxdH;4bYNqfHliYYPzZZk> MnjUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{whUUN3MDC9JFIh|ryPLh?= M4TGRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEi4NFY4Lz5zN{C4PFA3PzxxYU6=
Sf9 cells M1HJT2Z2dmO2aX;uJIF{e2G7 M3\ndFEhdWmw MXvJcohq[mm2aX;uJI9nKGi3bXHuJGZ6diCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIFnfGW{IEGgcYlvKGK7IFXMTXNCKGmwIIDy[ZNmdmOnIH;mJFEhfW2xbD;MJGFVWCxiSVO1NEA:KDBwMEC1JO69VS5? NH\CPGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells NGHLfFNHfW6ldHnvckBie3OjeR?= MWiyNEBucW6| NU[wUWhjUW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAzOCCvaX7zJIJ6KEWOSWPBJIlvKHC{ZYPlcoNmKG:oIEGgeY1wdC:OIFHUVEwhUUN3MDC9JFAvODF3IN88UU4> NYC3WmMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVU5PTNpPkG3N|E2QDV|PD;hQi=>
Sf9 cells NGrleVJHfW6ldHnvckBie3OjeR?= NV3yR|NwOSCvaX6= NHnEN4pKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFEhdWmwIHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlA{PiEQvF2u MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
Sf9 cells NUfBZmVSTnWwY4Tpc44h[XO|YYm= MkfyNlAhdWmwcx?= MkDnTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCyNEBucW6|IHL5JGVNUVODIHnuJJBz\XOnbnPlJI9nKDFyIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlA5OyEQvF2u NIrKelY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells MYHGeY5kfGmxbjDhd5NigQ>? NHHzOnYyKG2rbh?= NX;zXWtCUW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAyKG2rbjDifUBGVEmVQTDpckBxemW|ZX7j[UBw\iBzMECgeY1wdC:OIFHUVEwhUUN3MDC9JFAvOjdizszNMi=> NFnW[Fc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells NH\MXJFHfW6ldHnvckBie3OjeR?= MUeyNEBucW6| NHjJUWdKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFIxKG2rboOgZpkhTUyLU1GgbY4heHKnc3XuZ4Uhd2ZiMUCwJJVud2xxTDDBWHAtKEmFNUCgQUAxNjVzIN88UU4> NW\jdXlYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVU5PTNpPkG3N|E2QDV|PD;hQi=>
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MCF7 cells M3jKbGZ2dmO2aX;uJIF{e2G7 NYnaPZQ{UW6qaXLpeIlwdiCxZjDQT2EhcW5iaIXtZY4hVUOINzDj[YxteyCkeTDhdpJigS2kYYPl[EBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzKM7:TT6= MlXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NU[zOlkoRjF6NkW2N|Y6RC:jPh?=
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Sf9 cells MnnHSpVv[3Srb36gZZN{[Xl? NYXld4FFUW6qaXLpeIlwdiCxZjDoeY1idiCcQWC3NEBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|LDDJR|UxKD1iMD6wOVMh|ryPLh?= NWT4eVc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NlM4QDRpPkG4PFI{Pzh2PD;hQi=>
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insect Sf21 cells NGTDZXdHfW6ldHnvckBie3OjeR?= NFHLTIlKdmirYnn0bY9vKG:oIFrBT|Mh\XiycnXzd4VlKGmwIHnud4VkfCCVZkKxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYnnveIlvgWyjdHXkJJN2[nO2cnH0[UBxcG:|cHjvdplt[XSrb36sJGlEPTBiPTCwMlAxPiEQvF2u M3zTclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
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Jurkat cells MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mnj2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDKRWs{KGW6cILld5NqdmdiaIXtZY4hemW|dHnu[{BLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkOxNUDPxE1w NWP1NXNURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlczODNpPkG5OFI4OjB|PD;hQi=>
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HepG2 cells MofISpVv[3Srb36gZZN{[Xl? NX;MVpo4PDhiaB?= M2DQTGlvcGmkaYTpc44hd2ZidHXsc41memG|ZTDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgWHJCWC2SQ2KtSWxKW0FuIFnDOVAhRSB6LkOg{txONg>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ5OUi2OEc,OjN{N{m4OlQ9N2F-
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HepG2 cells NYHjO3pxS3m2b4TvfIlkcXS7IHHzd4F6 MkXGR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMtKEWFNUCgQUAzKM7:TT6= MkTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MU[zNVcoRjJ3M{G2N|E4RC:jPh?=
Raji cells M17uXGN6fG:2b4jpZ4l1gSCjc4PhfS=> M4LUSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJicmliY3XscJMtKEWFNUCgQUAzKM7:TT6= M1nx[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
BJ cells NX;3RlNMS3m2b4TvfIlkcXS7IHHzd4F6 NHP6cHFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDUiClZXzsd{whTUN3MDC9JFIh|ryPLh?= MmLwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MU[zNVcoRjJ3M{G2N|E4RC:jPh?=
HEK293 cells NWrIW5p7S3m2b4TvfIlkcXS7IHHzd4F6 NVH2WotHS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBGSzVyIE2gNkDPxE1w NYS3eWR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
A549 cells NVXYSphbS3m2b4TvfIlkcXS7IHHzd4F6 NXTIO4llPDhiaB?= M33lTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODJizszNMi=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NUmzOEc,OjV6MkW5N|Q9N2F-
HeLa cells NHfuVHVEgXSxdH;4bYNqfHliYYPzZZk> NXvxWIxnPDhiaB?= NILUdWJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{NTFOwG0v NY\2TZoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlU6OzRpPkK1PFI2QTN2PD;hQi=>
PC3 cells Mm[zR5l1d3SxeHnjbZR6KGG|c3H5 NVPzSZZ5PDhiaB?= Mk[1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGM{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA{OSEQvF2u MoGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
MGC-803 cells MnP4SpVv[3Srb36gZZN{[Xl? MUGyOEBp M3\CSWlvcGmkaYTpc44hd2ZidHXsc41memG|ZTDpckBpfW2jbjDNS2MuQDB|IHPlcIx{KGGodHXyJFI1KGi{czDifUBVWkGSLWDDVk1GVEmVQTDhd5NigSxiSVO1NEA:KDhwOUeg{txONg>? M3:5[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUCwOlU3Lz5{NkmwNFY2PjxxYU6=
Sf9 insect cells MmrjSpVv[3Srb36gZZN{[Xl? NH;uWGQyOCCvaX7z NXO2cIhSUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXCC2YXfn[YQhemWlb33ibY5idnRiZoXscE1t\W6pdHigbJVu[W5iQ1jLNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgV4Y6KGmwc3XjeEBk\WyuczDh[pRmeiBzMDDtbY5{KGK7IHz1cYlv\XOlZX70JGFFWC2JbH:gZZN{[XluIFnDOVAhRSByLkCwNVIh|ryPLh?= MknzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEmyNVEoRjJ5MEi5NlEyRC:jPh?=
Sf21 cells MnvmSpVv[3Srb36gZZN{[Xl? MYW2NEBucW6| M{i1emlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSlHLNkBmgHC{ZYPz[YQhcW5iU3[yNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iVXzp[4h1NUODR1HHRWlGXESNRWnZWHZMTCCyaH;zdIhwenmuYYTpc44heHKnLXnuZ5Vj[XSnZDDi[YZwemVic4Xid5Rz[XSnIHHk[Il1cW:wIHHu[EBu\WG|dYLl[EBi\nSncjC2NEBucW6|IHL5JGxCVkOHIHTleIVkfGmxbjDt[ZRpd2RuIFnDOVAhRSByLkCwNkDPxE1w NVH1[YRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexN|c{PTlpPkK3NVM4OzV7PD;hQi=>
Neuro2a cells M1XVU2N6fG:2b4jpZ4l1gSCjc4PhfS=> NFftTo84OiCq NUnLdo1XS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVmW3cn:yZUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wNVE{KM7:TT6= M4XROlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUWyOFI4Lz5{OEG1NlQzPzxxYU6=
MDA-MB-231 cells NYjjZ2tuTnWwY4Tpc44h[XO|YYm= Mli0NVYhcA>? MoLQTY5pcWKrdHnvckBw\iC{ZYTpco9jdGG|dH;tZUBxem:2ZXnuJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDF4IHjyd{BjgSCKb3XjbJN1KDN|M{SyJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFY3KM7:TT6= Mof0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{GzOFIoRjJ6NEOxN|QzRC:jPh?=
Sf9 insect cells NX21WnNlTnWwY4Tpc44h[XO|YYm= M4joZ|IxKG2rboO= NWTIcZgzUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDOMZRmem2rbnHscJkhT1OWLYTh[4dm\CCHR1\SJGw5PTiUL2S3PVBON0N5OUfTJJRzcXCuZTDteZRidnRiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwIGPmPUBqdnOnY4SgZ4VtdHNicILlbY5kfWKjdHXkJIZweiB{MDDtbY5{KG[xbHzve4VlKGK7IHHk[Il1cW:wIH;mJHs{O1CfLVHUVEBu\WG|dYLl[EBi\nSncjCyJIhzeyCkeTDmbYx1\XJvYnnu[Ilv\yCvZYToc4QtKEmFNUCgQUAxNjByMEK5JO69VS5? MnvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2OEKxOVEoRjJ6NEiyNVUyRC:jPh?=
HEK293 cells MVnDfZRwfG:6aXPpeJkh[XO|YYm= MYC3NkBp MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncjDHcI8hdHWvaX7ld4NmdnRiYYPzZZktKEOFNUCgQUAxNjByM{W0JO69VS5? NFfoUYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[yOFcxOSd-Mki2NlQ4ODF:L3G+
BT549 cells M1Hxc2N6fG:2b4jpZ4l1gSCjc4PhfS=> NHS5d28zPCCq MmTER5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wPEDPxE1w MkW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5MEW0N|IoRjJ6N{C1OFMzRC:jPh?=
MDA-MB-468 cells NHW2bFJEgXSxdH;4bYNqfHliYYPzZZk> NXfHTWxiOjRiaB?= Mn3JR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTR4ODDj[YxteyCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjF3IN88UU4> NXPzPHNNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
MDA-MB-231 cells NHvze2lEgXSxdH;4bYNqfHliYYPzZZk> NXTW[XFlOjRiaB?= M3K4OmN6fG:2b4jpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NDFOwG0v NFHKbGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEewOVQ{Oid-Mki3NFU1OzJ:L3G+
Vero cells MULDfZRwfG:6aXPpeJkh[XO|YYm= MXqyJIRigXN? MljkR5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgNkBl[Xm|IHL5JINmdGxvdHn0[ZIu\2yxIHz1cYlv\XOlZX70JIF{e2G7LDDUR|UxKD1iMD6wNFI1KM7:TT6= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd4M{[0OUc,Ojh5NkO2OFU9N2F-
L929 cells MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MVi1JIRigXN? MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEx7MkmgZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxKD1iMD6yJO69VS5? NEX4WZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
KB-3-1 cells M4\WV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1\tSVUh\GG7cx?= MorJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNQj2zMVEh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
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HepG2 cells NUHDVmptS3m2b4TvfIlkcXS7IHHzd4F6 Mn7DNVAxKHWP MUezJIRigXN? NVfkd4s4S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDNiZHH5d{BjgSCFZXzsWIl1\XJvR3zvJIx2dWmwZYPj[Y51KGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iMD6wNVgh|ryPLh?= NUL2UVZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5PVMyODZpPkK4PVk{OTB4PD;hQi=>
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A549 cells M3nHc2N6fG:2b4jpZ4l1gSCjc4PhfS=> NYDreoF[QTZiaB?= NV7EfHVlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5yNDFOwG0v M1rI[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
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HT-29 cells NWHNN5NZS3m2b4TvfIlkcXS7IHHzd4F6 NInQS2Q6PiCq NF;ZclVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5zNTFOwG0v MmX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
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HCT116 cells NEDVNWNEgXSxdH;4bYNqfHliYYPzZZk> M{TaNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOC5yNUWg{txONg>? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNkc,Ojl|OEmxNlI9N2F-
insect cells NFva[ZFHfW6ldHnvckBie3OjeR?= MonGN|AhdWmwcx?= MoXaTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCSRFfGVoJmfGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyC3c3nu[{BWdGmpaIStVI9tgSCJQWTbSWF[MDF8MUqxLX1vKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODByN{[g{txONg>? Mm\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
Sf9 insect cells NI\jc29HfW6ldHnvckBie3OjeR?= Mn;IOlAhdWmwcx?= NHP5NXNKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFvEVkBmgHC{ZYPz[YQhcW5iU3[5JIlve2WldDDj[YxteyC3c3nu[{BWdGmpaIStR2FISUeDSVXUSGtGYVmWVlvEJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KEyDTlPFJI1mfGixZDygTWM2OCB;IECuNFAzOSEQvF2u M1u3bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
insect cells NXvPSG1JTnWwY4Tpc44h[XO|YYm= NVnsWXQ2OTBibXnudy=> MVfJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKFO{YzDlfJBz\XO|ZXSgbY4hcW6|ZXP0JINmdGy|IIXzbY5oKFWuaXfoeE1Rd2y7IFfBWHtGSVlqMUqxPlEqZW5iYYOgd5Vje3S{YYTlJIFnfGW{IEGwJI1qdnNiYomgUGFPS0VibXX0bI9lNCCLQ{WwJF0hOC5yMEWg{txONg>? NXfjVmRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
insect cells Ml;nSpVv[3Srb36gZZN{[Xl? MlLsNVUhdWmwcx?= M4DidmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iQ1TLNU9EgWOuaX6gRkBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGXsbYdpfC2FRl\LUmlXXFCUVGDQVHNST0tvYX3p[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDF3IH3pcpMh[nliTFHOR2UhdWW2aH;kMEBKSzVyIE2gNE4xOjVizszNMi=> Mkf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells MVXGeY5kfGmxbjDhd5NigQ>? NILjRWUyPSCvaX7z MlfnTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFRFuxM2N6[2yrbjDCJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiVXzp[4h1NUOIRlvOTXZVWFKWUGDQV3FIUy2jbXnk[UBieyC|dXLzeJJifGViYX\0[ZIhOTVibXnud{BjgSCOQV7DSUBu\XSqb3SsJGlEPTBiPTCwMlAzPSEQvF2u MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
Sf9 insect cells NViyWVBtTnWwY4Tpc44h[XO|YYm= NGToZ4syPSCvaX7z MXXJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE[udEGg[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LWTLJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFE2KG2rboOgZpkhVEGQQ1WgcYV1cG:mLDDJR|UxKD1iMD6wN|Uh|ryPLh?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
Sf21 insect cells NEjDNYdHfW6ldHnvckBie3OjeR?= NIfjb2oyPSCvaX7z MkHvTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCDdYLvdoEuSSCneIDy[ZN{\WRiaX6gV4YzOSCrboPlZ5Qh[2WubIOgeZNqdmdiVXzp[4h1NVKUUmPMUGUh[XNic4Xid5Rz[XSnIHHmeIVzKDF3IH3pcpMh[nliTFHOR2UhdWW2aH;kMEBKSzVyIE2gNE4xOzlizszNMi=> MlXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells NIC3XmVHfW6ldHnvckBie3OjeR?= Mn\KOlAhdWmwcx?= MXrJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEGNVEGg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczD1d4lv\yCFUlXCeIll\SCjczDzeYJ{fHKjdHWgZYZ1\XJiNkCgcYlveyCkeTDMRW5ETSCvZYToc4QtKEmFNUCgQUAxNjB3NjFOwG0v Mo\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
Sf9 insect cells MXfGeY5kfGmxbjDhd5NigQ>? NYTaXpVMPjBibXnudy=> Mo\4TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCIQVug[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LWTLJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkhVEGQQ1WgcYV1cG:mLDDJR|UxKD1iMD6wOlIh|ryPLh?= MnPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells MWHGeY5kfGmxbjDhd5NigQ>? MoPGOlAhdWmwcx?= MXHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEGETDDlfJBz\XO|ZXSgbY4hcW6|ZXP0JINmdGy|IIXzbY5oKFWuaXfoeE1VUyCyZYD0bYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiB4MDDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvOjlizszNMi=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
insect cells M2f3fGZ2dmO2aX;uJIF{e2G7 M4[zdVYxKG2rboO= NYXaWXdMUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDjMW1GXCCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{KHW|aX7nJHVtcWeqdD3DRWdCT0GLRWTET2V[YVSYS1SgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkhVEGQQ1WgcYV1cG:mLDDJR|UxKD1iMD6zNUDPxE1w NYHkOlRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
PC3 cells MYrDfZRwfG:6aXPpeJkh[XO|YYm= M3nrflczKGh? NGTDV2VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvODN7IN88UU4> NYX4U4JuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OVgyOTlpPkK5OVU5OTF7PD;hQi=>
Sf9 cells NELC[IJHfW6ldHnvckBie3OjeR?= MmfvOFUhdWmwcx?= MVfJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKENvdHXycYlv[WxiSHnzMZRi\2enZD;OMZRmem2rbnHsJGdUXC22YXfn[YQhTUeIUjCoOlY5KHSxIEGyNVAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBU\jliY3XscJMhfXOrbnegVI9tgS1qR3z1MEBVgXJrIHHzJJN2[nO2cnH0[UBu\WG|dYLl[EBi\nSncjC0OUBucW63dHXzJIJ6KEurbnHz[U1IdG9ibIXtbY5me2OnboSgZZN{[XluIFnDOVAhRSByLkC0O{DPxE1w NFnuUIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwNlcyQSd-Mkm5NFI4OTl:L3G+
Sf9 cells MniySpVv[3Srb36gZZN{[Xl? NXrQOphMPDVibXnudy=> MXrJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiR2PUMZRi\2enZDDISXIzKCh4N{mgeI8hOTJ3NTDy[ZNq\HWnczmg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIGPmPUBk\WyuczD1d4lv\yCSb3z5MUhIdHVuIGT5dkkh[XNic4Xid5Rz[XSnIH3lZZN2emWmIHHmeIVzKDR3IH3pcpV1\XNiYomgT4lv[XOnLVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhUUN3MDC9JFAvODZyODFOwG0v MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyMkexPUc,Ojl7MEK3NVk9N2F-
HT-29 cells NFvq[mxHfW6ldHnvckBie3OjeR?= MnvxN{Bp NUfQXlBqUW6qaXLpeIlwdiCxZjDtbZRw[2ixbnTybYFtKG2nbXLyZY5mKHCxdHXueIlidCCrbjDoeY1idiCKVD2yPUBk\WyuczDh[pRmeiB|IHjyd{BjgSCMQz2xJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFIh|ryPLh?= MknkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNUexOVUoRjNyMEW3NVU2RC:jPh?=
Sf9 cells NFrWc49HfW6ldHnvckBie3OjeR?= M4rD[2lvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMjCoNUB1dyBzNEmwJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEWzJO69VS5? M3fuV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyME[3N|U5Lz5|MEC2O|M2QDxxYU6=
Sf9 cells M{DEWmZ2dmO2aX;uJIF{e2G7 M3X3[GlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMjCoNUB1dyBzNEmwJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEWzJO69VS5? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB4N{O1PEc,OzByNkezOVg9N2F-
Sf9 cells MkfESpVv[3Srb36gZZN{[Xl? NXn3TJdzUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGZNSUdvdHHn[4VlKGi3bXHuJIZ2dGxvbHXu[5RpKEOGS{GzJEgyKHSxIEG1NVIhemW|aXT1[ZMqN05vdHXycYlv[WxiSHnzMZRi\2enZDDDfYNMKChzIITvJFU5OCC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gRXRRNWSncHXu[IVvfCCXTHnnbJQuPEVvQmCxJEhVcHJ|Nz;UbJI1Pilic4Xid5Rz[XSnIIDldJRq\GVicHjvd5Bpd3K7bHH0bY9vKHC{LDDJR|UxKD1iMD6wOVch|ryPLh?= NV7hc4xVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOlc{PThpPkOwNFY4OzV6PD;hQi=>
Sf9 cells NFLVdJlHfW6ldHnvckBie3OjeR?= MUPJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTNiKEGgeI8hOTVzMjDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3NzFOwG0v NEHMfpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC2O|M2QCd-M{CwOlc{PTh:L3G+
HCT116 cells NF;U[3REgXSxdH;4bYNqfHliYYPzZZk> NX3RboVbPDhiaB?= M1Hpe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wN{DPxE1w MkjPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzME[yPVEoRjNyMUC2NlkyRC:jPh?=
Huh7.5 cells NX71eGJiS3m2b4TvfIlkcXS7IHHzd4F6 M3:2XFQ5KGh? NUXDemtXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWqNz61JINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlYh|ryPLh?= NGHOdpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEGwOlI6OSd-M{CxNFYzQTF:L3G+
K562 cells NHfMO3lEgXSxdH;4bYNqfHliYYPzZZk> Mn;EOFghcA>? NFfFWXZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkeg{txONg>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODFyNkK5NUc,OzBzME[yPVE9N2F-
LO2 cells MlL4R5l1d3SxeHnjbZR6KGG|c3H5 MkTJOFghcA>? MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMU|Ih[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFkvPSEQvF2u Ml71QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzME[yPVEoRjNyMUC2NlkyRC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

24174874 15121855 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

25215174 22684244 19880522 15140398 19840952 15121855
体内研究 在局部贫血前,使用Staurosporine(0.1-10 ng)预处理局部贫血沙鼠和大鼠模型,可防止神经元损伤,这种作用存在剂量依赖性,说明CAl锥体细胞死亡涉及PKC。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
细胞实验:[4]
- 合并
  • Cell lines: PC12
  • Concentrations: 溶于DMSO,终浓度为1 μM
  • Incubation Time: ~32 小时
  • Method: 使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合,然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞,测定凋亡细胞百分数。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 短暂局部贫血的雄性Mongolian沙鼠或雄性 Wistar大鼠
  • Dosages: ~10 ng
  • Administration: 定向处理到海马双边CA1子域
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 466.53
化学式

C28H26N4O3

CAS号 62996-74-1
储存条件 粉状
溶于溶剂
别名 CGP 41251

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID