Staurosporine (AM-2282)

For research use only. Not for use in humans.

目录号:S1421 别名: Antibiotic AM-2282, STS 中文名称:星形孢菌素

Staurosporine (AM-2282) Chemical Structure

CAS No. 62996-74-1

Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。

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RMB 2186.89 现货
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RMB 12203.1 现货
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客户使用Selleck生产的Staurosporine (AM-2282)发表文献105篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Staurosporine (AM-2282, Antibiotic AM-2282, STS)是一种有效的PKC抑制剂,在无细胞试验中作用于PKCα,PKCγ 和PKCη,IC50分别为2 nM,5 nM 和 4 nM,对PKCδ(20 nM)和PKCε(73 nM作用较弱,对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。
靶点
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外研究

Staurosporine 也强抑制HeLa S3细胞,IC50为4 nM。[1]Staurosporine 也抑制多种其他蛋白激酶,包括PKA, PKG, 磷酸激酶, S6 激酶, 肌球蛋白轻链激酶(MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, 和Syk , IC50分别为 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, 和 16 nM。[3] Staurosporine (1 μM) 诱导 PC12细胞90%以上凋亡,这种作用可被过量表达的Bcl-2抑制,或者zVAD-fmk, cycloheximide (10 μM) 及actinomycin D (5 μM)处理也可抑制。相应地, Staurosporine处理,诱导细胞内游离钙水平 [Ca2+]i快速且持久的提升,线粒体活性氧(ROS)的积累,及 随后的线粒体功能障碍。[4]通过caspase-8激活和Bid 分裂,功能性caspase-3的表达可增强Staurosporine诱导MCF7细胞死亡。[5] 1 μM Staurosporine处理,只部分抑制IL-3刺激的Bcl2 磷酸化,而完全阻断PKC调节的 Bcl2磷酸化。[6] Staurosporine 诱导人类包皮成纤维细胞AG-1518凋亡,根据溶酶体组织蛋白酶D调节的 细胞色素 c释放和 caspase 激活。[7] 除了激活传统线粒体凋亡通路, Staurosporine触发一种新型内在凋亡通路, 依赖Apaf-1存在时对caspase-9的激活。[8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 M2\5WGZ2dmO2aX;uJIF{e2G7 NGTHUm9Ed26lZX70doF1cW:wIILldZVqemWmIH\vdkBoem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBkd2yxbjDjZZJkcW6xbXGgZ4VtdCCuaX7lJGhEXDFzNjygTWM2OD14IH7NMi=> Mn7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3M{ezOFUoRjF3NUO3N|Q2RC:jPh?=
human PBMC NUfIelhsTnWwY4Tpc44h[XO|YYm= M1LZeVI1KGh? M3;s[HN2eHC{ZYPzbY9vKG:oIFnMNkBxem:mdXP0bY9vKGmwIHj1cYFvKFCETVOgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVE3KG6PLh?= M4SzeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUi1NFQ3Lz5zOEW4OVA1PjxxYU6=
endothelial cells NHq5THhHfW6ldHnvckBie3OjeR?= NFHQUIxG\m[nY4TpeoUh\G:|ZTDh[4FqdnO2IIDsZZNucW6xZ3XuJIFkfGm4YYTvdkBi[3Srdnn0fUB{fGmvdXzheIVlKGK7IIDoc5Jjd2xiZYP0[ZIhcW5iZX7kc5Rp\WyrYXygZ4VtdHNuIFXEOVAhRSB5LkWg{txONg>? NGPaSVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96N{C5NFk2Lz56N{C5NFk2RC:jPh?=
Sf9 insect cells MUTGeY5kfGmxbjDhd5NigQ>? NG\rTndKdmirYnn0bY9vKG:oIIDyc5RmcW5ia3nuZZNmKENqMUqxcYl5fHW{ZTDv[kBRU0NiYXzwbIEhLiCkZYThLUBmgHC{ZYPz[YQhcW5iU3[5JIlve2WldDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>? M2fVNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{O2NFUoRjl6N{O2NFU9N2F-
LoVo cells MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mkj0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhNd1[xIHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7MUWwOUc,OTF3OUG1NFU9N2F-
umbilical vein endothelial cells Ml\0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NX7tXYNRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjD1cYJqdGmlYXygeoVqdiCnbnTveIhmdGmjbDDj[YxteyBqSGXWSWN{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByNDFOwG0v NWrIb5hmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
ST-486 cells NX3BUFFLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4\Ne2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgZpVzc2m2IHz5cZBpd22jIHPlcIwhdGmwZTCoV3QuPDh4IHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByNzFOwG0v NVzoTHF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
DLD-1 cells NXX2T3VISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGOxbH;uJINidmOncjDj[YxtKGyrbnWgLGRNTC1zIHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByOTFOwG0v Mk\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3OUG1NFUoRjFzNUmxOVA2RC:jPh?=
HEK293 cells M4rBN2Z2dmO2aX;uJIF{e2G7 NFLkO|lKdmirYnn0bY9vKG:oIGDLR{1j\XSjIFnJJI1m\GmjdHXkJGlNNThicnXs[YF{\SCocn;tJGhGUzJ7MzDj[YxteyCkeTDFUGlUSSxiSVO1NEA:KDBwMEe3JO69VS5? MljPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NEGzN|EoRjF{OUSxN|MyRC:jPh?=
P19 cells NVzlTFJsTnWwY4Tpc44h[XO|YYm= M1zoPGlvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? M2fuUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells M4T3fmZ2dmO2aX;uJIF{e2G7 NXnCXGZYUW6qaXLpeIlwdiCxZjDQcIF1\WyndD3k[ZJqfmWmIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFIh|ryPLh?= MkDoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NGWzXYdHfW6ldHnvckBie3OjeR?= NEHtTFZKdmirYnn0bY9vKG:oIGDyc5RmcW5iS3nuZZNmKEFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xODRizszNMi=> M{jiS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells MkfrSpVv[3Srb36gZZN{[Xl? MkWwTY5pcWKrdHnvckBw\iCFYXzjbZVuN2OjbH3v[JVtcW5vZHXw[Y5l\W62IIDyc5RmcW5ia3nuZZNmKHS7cHWgTWkhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFYh|ryPLh?= M3TrPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells MlK5SpVv[3Srb36gZZN{[Xl? M{DYTGlvcGmkaYTpc44hd2ZiQ4njcIlvNWSncHXu[IVvfCCtaX7hd4UhOSCrbjDQNVkh[2WubIOsJGlEPTBiPTCwMlAxQCEQvF2u MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells M1LW[2Z2dmO2aX;uJIF{e2G7 MXXJcohq[mm2aX;uJI9nKF[jc3P1cIFzKGWwZH;0bIVtcWGuIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNVQh|ryPLh?= M1jMdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
HEK293 cells NUXEbYw5TnWwY4Tpc44h[XO|YYm= MUTJcohq[mm2aX;uJI9nKEmOLUigdoVt\WG|ZTDifUBJTUt{OUOgZ4VtdHNiZYjwdoV{e2mwZzDQT2Mu[mW2YUKsJGlEPTBiPTCwMlA4PyEQvF2u MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells M3HuRmZ2dmO2aX;uJIF{e2G7 NGTjbmpKdmirYnn0bY9vKG:oIFnud5VtcW5icnXj[ZB1d3Jia3nuZZNmKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNkDPxE1w NGj3d489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells MWHGeY5kfGmxbjDhd5NigQ>? NYDMdXZkUW6qaXLpeIlwdiCxZjDDZZNmcW5ia3nuZZNmKDFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNU41KM7:TT6= NVPuRo53RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
FL5.12-Akt1 cells M4ftWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NEfDPGdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGZNPS5zMj3Bb5QyKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkmg{txONg>? NX;ZWZQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFM3OjZpPkG2OFA{PjJ4PD;hQi=>
MiaPaCa-2 cells MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MkfERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNbYFR[UOjLUKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4{PyEQvF2u NUXQfmNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFM3OjZpPkG2OFA{PjJ4PD;hQi=>
FL5.12-Akt1 cells NUPm[2ZCTnWwY4Tpc44h[XO|YYm= M17UT2FkfGm4aYT5JIFo[Wmwc4SgS3NMOyCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= NXXnU2FCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFM3OjZpPkG2OFA{PjJ4PD;hQi=>
MiaPaCa-2 cells M3fzRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MorkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPaXHQZWNiNTJiY3XscJMtKEmFNUCgQUAxNjN5IN88UU4> NUS3SmxMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NVM4QDBpPkG2OFE{PzhyPD;hQi=>
FL5.12-Akt1 cells MXXGeY5kfGmxbjDhd5NigQ>? NYLnVIJZSWO2aY\peJkh[WejaX7zeEBIW0t|IIDoc5NxcG:{eXzheIlwdiCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= NFTuTlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSxN|c5OCd-MU[0NVM4QDB:L3G+
Sf21 cells M1TmUGZ2dmO2aX;uJIF{e2G7 NYPnNJpsUW6qaXLpeIlwdiCxZjDKRWs{KGW6cILld5Nm\CCrbjDT[lIyKGOnbHzzMEBKSzVyIE2gNE4xODZizszNMi=> MnLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyOEiwOVkoRjF5MEi4NFU6RC:jPh?=
U937 cells NXzN[VVrS3m2b4TvfIlkcXS7IHHzd4F6 NFfBZmFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{NCCLQ{WwJF0hOiEQvF2u NFToe5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{C4PFA3Pyd-MUewPFgxPjd:L3G+
Sf9 cells MWDGeY5kfGmxbjDhd5NigQ>? MYOxJI1qdg>? NVe4UHZrUW6qaXLpeIlwdiCxZjDoeY1idiCIeX6g[ZhxemW|c3XkJIlvKFOoOTDj[YxteyCjZoTldkAyKG2rbjDifUBGVEmVQTDpckBxemW|ZX7j[UBw\iBzIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlAxPSEQvF2u NHrheo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells M{\5Z2Z2dmO2aX;uJIF{e2G7 NUX4Znk4OjBibXnudy=> NHrFXJlKdmirYnn0bY9vKG:oIHj1cYFvKE[7bjDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{KGGodHXyJFIxKG2rboOgZpkhTUyLU1GgbY4heHKnc3XuZ4Uhd2ZiMTD1cY9tN0xiQWTQMEBKSzVyIE2gNE4xOTVizszNMi=> NFLiPXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{OxOVg2Oyd-MUezNVU5PTN:L3G+
Sf9 cells NHz0UJRHfW6ldHnvckBie3OjeR?= NULuPWhiOSCvaX6= M{XQTmlvcGmkaYTpc44hd2ZiaIXtZY4hTnmwIHX4dJJme3OnZDDpckBU\jliY3XscJMh[W[2ZYKgNUBucW5iYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOTBidX3vcE9NKEGWUDygTWM2OCB;IECuNFM3KM7:TT6= Mn64QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|MUW4OVMoRjF5M{G1PFU{RC:jPh?=
Sf9 cells NIrsdHZHfW6ldHnvckBie3OjeR?= NVu0V3JROjBibXnudy=> M2jucmlvcGmkaYTpc44hd2ZiaIXtZY4hTnmwIHX4dJJme3OnZDDpckBU\jliY3XscJMh[W[2ZYKgNlAhdWmwczDifUBGVEmVQTDpckBxemW|ZX7j[UBw\iBzMDD1cY9tN0xiQWTQMEBKSzVyIE2gNE4xQDNizszNMi=> M1vBflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{G1PFU{Lz5zN{OxOVg2OzxxYU6=
Sf9 cells MYDGeY5kfGmxbjDhd5NigQ>? M2fnU|EhdWmw MkLYTY5pcWKrdHnvckBw\iCqdX3hckBHgW5iZYjwdoV{e2WmIHnuJHNnQSClZXzsd{Bi\nSncjCxJI1qdiCkeTDFUGlUSSCrbjDwdoV{\W6lZTDv[kAyODBidX3vcE9NKEGWUDygTWM2OCB;IECuNlch|ryPLh?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
Sf9 cells NH74Z5lHfW6ldHnvckBie3OjeR?= MUOyNEBucW6| MXLJcohq[mm2aX;uJI9nKGi3bXHuJGZ6diCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIFnfGW{IEKwJI1qdnNiYomgSWxKW0FiaX6gdJJme2WwY3Wgc4YhOTByIIXtc4wwVCCDVGCsJGlEPTBiPTCwMlUyKM7:TT6= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzNzNUi1N{c,OTd|MUW4OVM9N2F-
A549 cells NVq2Uol7S3m2b4TvfIlkcXS7IHHzd4F6 NYi4PIQ6PzJiaB?= NXjaNnNLS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBu\XSqb3SsJGdKPTBiPTCwMlAxOjRizszNMi=> M3nZe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEi0O|c2Lz5zOES4OFc4PTxxYU6=
MDA-MB-231 cells Ml3yR5l1d3SxeHnjbZR6KGG|c3H5 M4LNVVczKGh? NFfBWolEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIH3leIhw\CxiR1m1NEA:KDBwMEC3NUDPxE1w M1PoWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEi0O|c2Lz5zOES4OFc4PTxxYU6=
HT29 cells MVXDfZRwfG:6aXPpeJkh[XO|YYm= MluzO|IhcA>? MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWFI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIH3leIhw\CxiR1m1NEA:KDBwMEGwPUDPxE1w NFfZVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES4OFc4PSd-MUi0PFQ4PzV:L3G+
MCF7 cells MoHQSpVv[3Srb36gZZN{[Xl? M2TH[GlvcGmkaYTpc44hd2ZiUFvBJIlvKGi3bXHuJG1ETjdiY3XscJMh[nliYYLyZZku[mG|ZXSg[ox2d3Knc3PlcoNmKGG|c3H5MEBMcSB;IECuNFA2KM7:TT6= M1vhN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkW2N|Y6Lz5zOE[1OlM3QTxxYU6=
MCF7 cells NV\BOWFPTnWwY4Tpc44h[XO|YYm= MoPGTY5pcWKrdHnvckBw\iCSS1GgbY4hcHWvYX6gUWNHPyClZXzsd{BjgSCjcoLhfU1j[XOnZDDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCyJO69VS5? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ3NkO2PUc,OTh4NU[zOlk9N2F-
Sf9 cells NVP6UIxOTnWwY4Tpc44h[XO|YYm= M2PoNmlvcGmkaYTpc44hd2ZiaIXtZY4hW3mtIHX4dJJme3OnZDDpckBU\jliY3XscJMtKEmFNUCgQUAxNjByMzFOwG0v NGLRUXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
Sf9 cells NI\sR|hHfW6ldHnvckBie3OjeR?= MnGyTY5pcWKrdHnvckBw\iCqdX3hckBbSVB5MDDlfJBz\XO|ZXSgbY4hW2Z7IHPlcIx{NCCLQ{WwJF0hOC5yNUOg{txONg>? NFfaXWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
insect cells NWrLenY6TnWwY4Tpc44h[XO|YYm= NHHOZZlKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IGDpcVEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BjgSCKVGLGMEBKSzVyIE2gNE4xOSEQvF2u NVeyc5ZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxO|kxPzZpPkG5NVc6ODd4PD;hQi=>
HT-29 cells MYnBdI9xfG:|aYOgZZN{[Xl? M{S0cFIhcA>? NF\RXnBKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFjUMVI6KGOnbHzzJIF{e2W|c3XkJIF{KGOqYX7n[UBqdiCvaYTvZ4hwdmS{aXHsJJRz[W6|bXXtZpJidmVicH;0[Y51cWGuIHHmeIVzKDJiaILzJIJ6KEqFMTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgZZN{[XluIFXEOVAhRSByLkCwNlYh|ryPLh?= NUHmN5hDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlIzODZpPkG5OFIzOjB4PD;hQi=>
insect Sf21 cells MWTGeY5kfGmxbjDhd5NigQ>? MkPqTY5pcWKrdHnvckBw\iCMQVuzJIV5eHKnc4Pl[EBqdiCrboPlZ5QhW2Z{MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGKrb4Tpcplt[XSnZDDzeYJ{fHKjdHWgdIhwe3Cqb4L5cIF1cW:wLDDJR|UxKD1iMD6wNFYh|ryPLh?= Ml3VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MkeyNFMoRjF7NEK3NlA{RC:jPh?=
Jurkat cells MkPQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NH;kU2tCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDJUFIue3SrbYXsZZRm\CCqdX3hckBLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkC3NUDPxE1w M4P6S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
Jurkat cells M13sNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NIK0fnJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDoeY1idiC{ZYP0bY5oKEq3cnvheEBk\WyuczygTWM2OCB;IECuN|EyKM7:TT6= NX\4NpA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlczODNpPkG5OFI4OjB|PD;hQi=>
HUE cells NVTFTFViTnWwY4Tpc44h[XO|YYm= NUjKTVhlQTBibXnudy=> MUjJcohq[mm2aX;uJI9nKF[HR1\SNkBqdiCKVVWgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCYRVfGMYlv\HWlZXSgZZV1d3Cqb4PwbI9zgWyjdHnvckB1emWjdHXkJIZweiB7MDDtbY5{KGKnZn;y[UBXTUeIIHPoZYxt\W6pZTDifUBGVEmVQTygTWM2OCB;IECuNFch|ryPLh?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF5MEG2N{c,OjBzN{CxOlM9N2F-
Sf9 cells Mn;DSpVv[3Srb36gZZN{[Xl? NF7TeoE3OCCvaX7z NFLnTGdKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFfTWE1nfXOnZDDOTWsh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDh[pRmeiB4MDDtbY5{KGK7IILh[IlwdWW2cnnjJJBzd3SnaX6gb4lv[XOnIHHzd4F6NCCLQ{WwJF0hOi5|IN88UU4> NYLENHBiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1PFA2PTJpPkKwOVgxPTV{PD;hQi=>
Sf21 cells MXfGeY5kfGmxbjDhd5NigQ>? NIjHPFE{OCC3TR?= NF;sSXFKdmirYnn0bY9vKG:oIHj1cYFvKEOKS{Kgb4lv[XOnIHX4dJJme3OnZDDpckBqdnOnY4SgV4YzOSClZXzsd{B2e2mwZzDwbI9{eGixLVPSSWJ1cWSnIHHzJJN2[nO2cnH0[UBifCB|MDD1UUwhUUN3MDC9JFAvODJ5IN88UU4> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|NEe5Okc,OjF|M{S3PVY9N2F-
Sf21 cells Mn\RSpVv[3Srb36gZZN{[Xl? MX2zNEB2VQ>? NH;YZWhKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFnLT{1idHCqYTDlfJBz\XO|ZXSgbY4hcW6|ZXP0JHNnOjFiY3XscJMhfXOrbnegdIhwe3Cqbz3VcIlocHRvSXvhdJBiSi2jbIDoZUBieyC|dXLzeJJifGViYYSgN|AhfU1uIFnDOVAhRSByLkCzPUDPxE1w M4PFcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O0O|k3Lz5{MUOzOFc6PjxxYU6=
Sf21 cells NVfFZlFPTnWwY4Tpc44h[XO|YYm= M1i5blMxKHWP NWWyTWRUUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDJT2su[mW2YTDlfJBz\XO|ZXSgbY4hcW6|ZXP0JHNnOjFiY3XscJMhfXOrbnegdIhwe3Cqbz3VcIlocHRvSXvhdJBiSi2jbIDoZUBieyC|dXLzeJJifGViYYSgN|AhfU1uIFnDOVAhRSByLkS5JO69VS5? M2jlPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O0O|k3Lz5{MUOzOFc6PjxxYU6=
HeLa cells MVXDfZRwfG:6aXPpeJkh[XO|YYm= NGTDWno1QCCq NFG4XWNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjByMECwOEDPxE1w MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN6OEG5NUc,OjF|OEixPVE9N2F-
MCF7 cells NGP1NJBEgXSxdH;4bYNqfHliYYPzZZk> NGTuemQ1QCCq NHr4eHBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB3IN88UU4> Ml\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|OEixPVEoRjJzM{i4NVkyRC:jPh?=
HT-29 cells MVfGeY5kfGmxbjDhd5NigQ>? NGLrNo4zKGh? MmfaSYZn\WO2IH;uJI1qfG:laH;u[JJq[WxibXXtZpJidmVicH;0[Y51cWGuIHnuJIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEKgbJJ{KHW|aX7nJGpENTFic4ThbY5qdmdiYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xODJizszNMi=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR{OEO3OUc,OjF2MkizO|U9N2F-
HeLa cells MmO2R5l1d3SxeHnjbZR6KGG|c3H5 MUi0PEBp MlLYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xODByMESg{txONg>? M{n3fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEi4OlU2Lz5{MUS4PFY2PTxxYU6=
MCF7 cells NFPqWFREgXSxdH;4bYNqfHliYYPzZZk> M4DBTFQ5KGh? M3TZe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODVizszNMi=> MmLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2OEi2OVUoRjJzNEi4OlU2RC:jPh?=
HT-29 cells NInXTJpHfW6ldHnvckBie3OjeR?= Mnz0TY5pcWKrdHnvckBw\iCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJJBwfGWwdHnhcEBqdiCqdX3hckBJXC1{OTDj[YxteyC3c3nu[{BLSzFiZInlJJN1[WmwaX7nJIJ6KG[udX;y[ZNk\W6lZTDwcIF1\SC{ZXHk[ZIh[XO|YYmsJGlEPTBiPTCwMlAxOjVizszNMi=> M4XsflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlk{Lz5{MUWxN|I6OzxxYU6=
SF268 cells MVvDfZRwfG:6aXPpeJkh[XO|YYm= M4nFUFQ5KGh? NXLRSVFLS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0Z{NkigZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NEA:KDBwMES0JO69VS5? M1jC[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlk1Lz5{MUWxN|I6PDxxYU6=
CHOK1 cells NVT5coE2S3m2b4TvfIlkcXS7IHHzd4F6 NH3zcnY1QCCq NGD3SoFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEUE:NMTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwJF0hOC5zMzFOwG0v M4DMbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlk1Lz5{MUWxN|I6PDxxYU6=
H460 cells MYrDfZRwfG:6aXPpeJkh[XO|YYm= NV7hVFNpPDhiaB?= NVvR[|NHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDR4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwJF0hOy54IN88UU4> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K5OEc,OjF3MUOyPVQ9N2F-
HT-29 cells MnLYR5l1d3SxeHnjbZR6KGG|c3H5 NH7SUpg1QCCq MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyIE2gN{43KM7:TT6= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K5OEc,OjF3MUOyPVQ9N2F-
MCF7 cells NVvaR|FkS3m2b4TvfIlkcXS7IHHzd4F6 MlPXOFghcA>? MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGdKPTBiPTCxNUDPxE1w NXz1Opc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzQTRpPkKxOVE{Ojl2PD;hQi=>
SW1116 cells NGPIdIZHfW6ldHnvckBie3OjeR?= MljLNlQhcA>? M1LhbGlvcGmkaYTpc44hd2ZidHXsc41memG|ZTDpckBpfW2jbjDTW|EyOTZiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHRTSVBvUFPSMWVNUVODIHHzd4F6NCCLQ{WwJF0hQC5|MjFOwG0v MlHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkK1NlMoRjJzOU[yOVI{RC:jPh?=
HT-29 cells M2HyfmFxd3C2b4Ppd{Bie3OjeR?= MYCyJIg> M1\he2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSGStNlkh[2WubIOgZZN{\XO|ZXSgdoVlfWO2aX;uJI9nKG2rdH;jbI9v\HKrYXygcYVu[nKjbnWgdI91\W62aXHsJIFnfGW{IEKgbJJ{KGK7IIXzbY5oKEqFMTDzeIFqdmmwZzDifUBndHWxcnXzZ4Vv[2ViY3XscE1j[XOnZDDhd5NigSxiRVS1NEA:KDBwMECyOkDPxE1w MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl5M{GwNUc,OjF7N{OxNFE9N2F-
LoVo cells NYP2cGUzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mn7COFghfG9iN{KgbC=> NEPXVlZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzvWo8h[2WubIOgZYZ1\XJiNEigeI8hPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMECxJO69VS5? M3TUOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUiyPVI6Lz5{MkG4NlkzQTxxYU6=
ST486 cells Ml7aRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MorEOFghfG9iN{KgbC=> MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOWNEi2JINmdGy|IHHmeIVzKDR6IITvJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjByNzFOwG0v MlTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzOEK5NlkoRjJ{MUiyPVI6RC:jPh?=
DLD1 cells MmHuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHK5V|g1QCC2bzC3NkBp MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESOREGgZ4VtdHNiYX\0[ZIhPDhidH:gO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODB7IN88UU4> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF6MkmyPUc,OjJzOEK5Nlk9N2F-
B16F10 cells MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MnTPOFghcA>? MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKEJzNl[xNEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj64NkDPxE1w MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVyM{O2OEc,OjJ3MEOzOlQ9N2F-
MCF7 cells M2foN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVO0PEBp MmPlRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB|LkC3JO69VS5? Mn\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3MEOzOlQoRjJ{NUCzN|Y1RC:jPh?=
A431 cells NHTTeXJEgXSxdH;4bYNqfHliYYPzZZk> M{DRWlI1KGh? MlrmR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{Bi\nSncjCyOEBpenNidYPpcochSW6wZYjpckBX\U[LVFOvdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB5IN88UU4> NEfte2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
MCF7 cells M2XhfmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2fvV|I1KGh? NU\2R4tDS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkAzPCCqcoOgeZNqdmdiQX7u[ZhqdiCYZV\JWGMweHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkG4JO69VS5? NETQe5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
BJ cells MofqR5l1d3SxeHnjbZR6KGG|c3H5 NVXm[ZF5PzJiaB?= NWW4bFlsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkpiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNidGOnaX6gRW0h[XO|YYmsJGlEPTBiPTCwMlAxOiEQvF2u NV3hZYpwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
CEM cells M4DIeWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2W1VVczKGh? Mn;5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VOKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDDZYxk\WmwIFHNJIF{e2G7LDDJR|UxKD1iMD6wNlMh|ryPLh?= NHzvb2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyNVA5OSd-MkK5NlExQDF:L3G+
MCF7 cells NUf3c3g2S3m2b4TvfIlkcXS7IHHzd4F6 MWq3NkBp MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDDZYxk\WmwIFHNJIF{e2G7LDDJR|UxKD1iMD6wOlQh|ryPLh?= NHHVd|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyNVA5OSd-MkK5NlExQDF:L3G+
HeLa cells NGTrZ4ZEgXSxdH;4bYNqfHliYYPzZZk> NGPFdHc4OiCq MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDDZYxk\WmwIFHNJIF{e2G7LDDJR|UxKD1iMD6xO|Uh|ryPLh?= NVH6e2VNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
HepG2 cells MVnGeY5kfGmxbjDhd5NigQ>? NF\Se4kzPCCq NWDuenI4UW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBVWkGSLWDDVk1GVEmVQTDhd5NigSxiSVO1NEA:KDRwMUSg{txONg>? NW\mUIVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVgxQTZpPkKzNFE5ODl4PD;hQi=>
SW1116 cells MmXpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MknhOFghcA>? MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZMUGxOkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJI1mfGixZDygTWM2OCB;IESuPVUh|ryPLh?= NH33TXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
HepG2 cells NVfuR2xSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYLNZ|VJPDhiaB?= NXTJVnFpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKG2ndHjv[EwhUUN3MDC9JFYvPzNizszNMi=> M{SzclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG4NFk3Lz5{M{CxPFA6PjxxYU6=
BGC823 cells M4KwS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MmLaOFghcA>? MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKJQ{iyN{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJI1mfGixZDygTWM2OCB;IE[uPFMh|ryPLh?= NHHGOlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
HeLa cells M4nYOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NGjacVA1QCCq NI\ZSZpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBu\XSqb3SsJGlEPTBiPTC5MlEzKM7:TT6= MmPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HCT116 cells NYPCdFFsTnWwY4Tpc44h[XO|YYm= NFf5b25KdmirYnn0bY9vKG:oIGTOTWsudWWmaXH0[YQhXEOIND;i[ZRiNWOjc3XpckB1emGwc3PybZB1cW:wIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BjgSCkZYThMYxi[3SjbXHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yM{mg{txONg>? NVK1dZQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|IxPjBpPkKzNlMzODZyPD;hQi=>
HepG2 cells MlXvSpVv[3Srb36gZZN{[Xl? MVm0PEBp NF\KTVhKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHRTSVBvUFPSMWVNUVODLDDJR|UxKD1iOD6zJO69VS5? NXXC[Jg6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|k5PjRpPkKzNlc6QDZ2PD;hQi=>
SW1116 cells M3HrdGZ2dmO2aX;uJIF{e2G7 MYmyOEBp NGDDWodKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gV3cyOTF4IHPlcIx{KGGodHXyJFI1KGi{czDifUBVWkGSLWDDVk1GVEmVQTDhd5NigSxiSVO1NEA:KDRwMUig{txONg>? NYHuZo5JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFY4PjFpPkK0Nlg3PzZzPD;hQi=>
KE-97 cells NIfpTnBEgXSxdH;4bYNqfHliYYPzZZk> NEPCVWU4OiCq NWPj[ohES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0VvOUegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRz\S2JbH:gcJVucW6nc3PlcpQh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkGzJO69VS5? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{OEK4N{c,OjR|MkiyPFM9N2F-
Jurkat cells M1q0dmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFjISVk4OiCq M2CxOmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGp2emujdDDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMUSg{txONg>? NX;YZ3BSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
HuH7 cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MmLIO|IhcA>? M2PpS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjJ|IN88UU4> M1e1fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K4Nlg{Lz5{NEOyPFI5OzxxYU6=
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MCF7 cells MkjCR5l1d3SxeHnjbZR6KGG|c3H5 NXHyNlY6PzJiaB?= M4rQUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjV{IN88UU4> MnTtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkiyPFMoRjJ2M{K4Nlg{RC:jPh?=
NIH/3T3 cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= NWrwXXpzQTZiaB?= MmTnR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5{IN88UU4> M{P0flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[xOVIyLz5{NEO2NVUzOTxxYU6=
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HT-29 cells MlzuR5l1d3SxeHnjbZR6KGG|c3H5 M4LLTFk3KGh? MnnYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NIDqWpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
8505C cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= NYfk[|VQQTZiaB?= MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC4OVA2SyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4MUWyNUc,OjR|NkG1NlE9N2F-
518A2 cells NX3GOpN4S3m2b4TvfIlkcXS7IHHzd4F6 MYm5OkBp MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4MUWyNUc,OjR|NkG1NlE9N2F-
MCF7 cells MXHDfZRwfG:6aXPpeJkh[XO|YYm= NUOwe4J4QTZiaB?= M1fQ[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvPCEQvF2u NI\ofm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
A549 cells NIP4bG1EgXSxdH;4bYNqfHliYYPzZZk> NEC1SYg6PiCq MknDR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE43KM7:TT6= MmPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkG1NlEoRjJ2M{[xOVIyRC:jPh?=
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HEK293 cells MojkR5l1d3SxeHnjbZR6KGG|c3H5 M1zuUVczKGh? NEHwOlBEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSnclfsc{Bie3OjeTygTWM2OCB;IECuNFU3KM7:TT6= NYWyWJF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlMzPjJpPkK0O|Y{OjZ{PD;hQi=>
HeLa cells MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYOyOEB1dyB2ODDo NFPGU4FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiMkSgeI8hPDhiaILzJIJ6KEOFSz24JIF{e2G7LDDJR|UxKD1iMj63NkDPxE1w MontQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5OUK4NVEoRjJ2N{myPFEyRC:jPh?=
A549 cells M4XVNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWqyOEB1dyB2ODDo MoLvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDJ2IITvJFQ5KGi{czDifUBES0tvODDhd5NigSxiSVO1NEA:KDNwMEWg{txONg>? M1rqcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{myPFEyLz5{NEe5NlgyOTxxYU6=
HepG2 cells NES3cnBEgXSxdH;4bYNqfHliYYPzZZk> MoT4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMtKEWFNUCgQUAzKM7:TT6= NW\obFZ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
Raji cells M2j5dWN6fG:2b4jpZ4l1gSCjc4PhfS=> Mof0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{whTUN3MDC9JFIh|ryPLh?= NHTsRm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOxOlMyPyd-MkWzNVY{OTd:L3G+
BJ cells Mnn4R5l1d3SxeHnjbZR6KGG|c3H5 MlHOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmoh[2WubIOsJGVEPTBiPTCyJO69VS5? M3vCVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
HEK293 cells MWnDfZRwfG:6aXPpeJkh[XO|YYm= MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNuIFXDOVAhRSB{IN88UU4> NYnmemdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
A549 cells Ml7IR5l1d3SxeHnjbZR6KGG|c3H5 MoPMOFghcA>? MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlAzKM7:TT6= MkHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
HeLa cells M37SWWN6fG:2b4jpZ4l1gSCjc4PhfS=> NETrV|k1QCCq NVjMTXdqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMkWg{txONg>? M3fndlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK1PVM1Lz5{NUiyOVk{PDxxYU6=
PC3 cells MlSzR5l1d3SxeHnjbZR6KGG|c3H5 NV6yXXlzPDhiaB?= MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFMyKM7:TT6= MmHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
MGC-803 cells MVPGeY5kfGmxbjDhd5NigQ>? NHr5dHMzPCCq NHfj[5lKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gUWdENThyMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhXFKDUD3QR3IuTUyLU1GgZZN{[XluIFnDOVAhRSB6Lkm3JO69VS5? NVzXNXNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5NFA3PTZpPkK2PVAxPjV4PD;hQi=>
Sf9 insect cells NY\BOo1qTnWwY4Tpc44h[XO|YYm= NFu1T3EyOCCvaX7z MXXJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiR2PUJJRi\2enZDDy[YNwdWKrbnHueEBnfWyuLXzlcod1cCCqdX3hckBEUEtzIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCVZkmgbY5{\WO2IHPlcIx{KGGodHXyJFExKG2rboOgZpkhdHWvaX7ld4NmdnRiQVTQMWdtdyCjc4PhfUwhUUN3MDC9JFAvODBzMjFOwG0v MoLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEmyNVEoRjJ5MEi5NlEyRC:jPh?=
Sf21 cells MVnGeY5kfGmxbjDhd5NigQ>? MlzhOlAhdWmwcx?= NGnJN5ZKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFrBT|Ih\XiycnXzd4VlKGmwIGPmNlEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIGXsbYdpfC2FQVfBS2FKTVSGS1XZXXRXU0RicHjvd5Bpd3K7bHH0bY9vKHC{ZT3pcoN2[mG2ZXSgZoVnd3KnIIP1ZpN1emG2ZTDh[IRqfGmxbjDhcoQhdWWjc4Xy[YQh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDk[ZRm[3Srb36gcYV1cG:mLDDJR|UxKD1iMD6wNFIh|ryPLh?= NFn4OFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GzO|M2QSd-MkexN|c{PTl:L3G+
Neuro2a cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= NYXJ[GxxPzJiaB?= Mli1R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUoV2em9{YTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMUGzJO69VS5? MnzqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzNUK0NlcoRjJ6MUWyOFI4RC:jPh?=
MDA-MB-231 cells M1vQWmZ2dmO2aX;uJIF{e2G7 M3;3c|E3KGh? NUDVWXNnUW6qaXLpeIlwdiCxZjDy[ZRqdm:kbHHzeI9u[SCycn;0[YlvKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDIc4VkcHO2IEOzN|QzKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODZ4IN88UU4> MkOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{GzOFIoRjJ6NEOxN|QzRC:jPh?=
Sf9 insect cells NFrufZRHfW6ldHnvckBie3OjeR?= NEPwcJAzOCCvaX7z MoDvTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCQLYTldo1qdmGubImgS3NVNXSjZ3fl[EBGT0[UIFy4OVhTN1R5OUDNM2M4QTeVIITybZBt\SCvdYThcpQh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlvKFOoOTDpcpNm[3RiY3XscJMheHKnaX7jeYJifGWmIH\vdkAzOCCvaX7zJIZwdGyxd3XkJIJ6KGGmZHn0bY9vKG:oIGuzN3BeNUGWUDDt[YF{fXKnZDDh[pRmeiB{IHjyd{BjgSCoaXz0[ZIu[mmwZHnu[{Bu\XSqb3SsJGlEPTBiPTCwMlAxODJ7IN88UU4> MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2OEKxOVEoRjJ6NEiyNVUyRC:jPh?=
HEK293 cells NFe4fJZEgXSxdH;4bYNqfHliYYPzZZk> NF;PXmY4OiCq MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncjDHcI8hdHWvaX7ld4NmdnRiYYPzZZktKEOFNUCgQUAxNjByM{W0JO69VS5? M1PMWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkK0O|AyLz5{OE[yOFcxOTxxYU6=
BT549 cells NFH5fpREgXSxdH;4bYNqfHliYYPzZZk> NV3DWG5uOjRiaB?= NHLITpFEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFU1QSClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA5KM7:TT6= MnrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5MEW0N|IoRjJ6N{C1OFMzRC:jPh?=
MDA-MB-468 cells NH3ld2dEgXSxdH;4bYNqfHliYYPzZZk> NWG3SVVxOjRiaB?= MmnCR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTR4ODDj[YxteyCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjF3IN88UU4> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDdyNUSzNkc,Ojh5MEW0N|I9N2F-
MDA-MB-231 cells MVTDfZRwfG:6aXPpeJkh[XO|YYm= M4OwXVI1KGh? NUPO[XV2S3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkK0JO69VS5? NF\WdlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEewOVQ{Oid-Mki3NFU1OzJ:L3G+
Vero cells NEHH[VlEgXSxdH;4bYNqfHliYYPzZZk> M{K4UVIh\GG7cx?= MmDvR5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgNkBl[Xm|IHL5JINmdGxvdHn0[ZIu\2yxIHz1cYlv\XOlZX70JIF{e2G7LDDUR|UxKD1iMD6wNFI1KM7:TT6= NVSxdIpyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3OlM3PDVpPkK4O|Y{PjR3PD;hQi=>
L929 cells NW\qO24ySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MkX5OUBl[Xm| NYHPeWxVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDMPVI6KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDXV3QuOSCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NYDXUZZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OTVpPkK4PVU3QTF3PD;hQi=>
KB-3-1 cells MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlrpOUBl[Xm| M1TKd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iS1KtN{0yKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDXV3QuOSCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u M1XPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVE2Lz5{OEm1OlkyPTxxYU6=
MCF7 cells MlXNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYm1JIRigXN? NGnRTFhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNU45KM7:TT6= NYfUNolPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OTVpPkK4PVU3QTF3PD;hQi=>
FS4-LTM cells NUXQc243SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NUW2dG1ZOjRiaB?= M2q2dWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRmO0MWxVVSClZXzsd{Bi\nSncjCyOEBpenNiYomgW3NVNTFiYYPzZZktKEmFNUCgQUAzNjhizszNMi=> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
Vero cells MoTtR5l1d3SxeHnjbZR6KGG|c3H5 NYrFTm5wOiCmYYnz MlH1R5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB{IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNFAzPCEQvF2u M2D4XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUmzNVA3Lz5{OEm5N|ExPjxxYU6=
HepG2 cells MVHDfZRwfG:6aXPpeJkh[XO|YYm= MY[xNFAhfU1? M3zZVFMh\GG7cx?= M4PJNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA{KGSjeYOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMEG4JO69VS5? MoPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7OUOxNFYoRjJ6OUmzNVA3RC:jPh?=
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518A2 cells NH3R[5JEgXSxdH;4bYNqfHliYYPzZZk> M2rrZlk3KGh? MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBGSzVyIE2gNE4xOyEQvF2u MkTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
A549 cells NHXrOYREgXSxdH;4bYNqfHliYYPzZZk> NIrHeWU6PiCq M3LFV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhTUN3MDC9JFAvODRizszNMi=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF7N{ezNEc,OjlzOUe3N|A9N2F-
MCF7 cells NUTIfY9VS3m2b4TvfIlkcXS7IHHzd4F6 NEjP[4U6PiCq NHn6NGJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEWFNUCgQUAxNjFizszNMi=> M4f0bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
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HT-29 cells M{fiOWN6fG:2b4jpZ4l1gSCjc4PhfS=> MnPDPVYhcA>? MlfxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhTUN3MDC9JFAvOTVizszNMi=> NVPmfGtoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxPVc4OzBpPkK5NVk4PzNyPD;hQi=>
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HCT116 cells NYXHRVF3S3m2b4TvfIlkcXS7IHHzd4F6 M4TieGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOC5yNUWg{txONg>? M3i5WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIzLz5{OUO4PVEzOjxxYU6=
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Sf9 cells M1zYe2Z2dmO2aX;uJIF{e2G7 Ml\NOFUhdWmwcx?= NIXqcFBKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTVJ{IDi2O|khfG9iMUK1OUBz\XOrZIXld{kh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJHNnQSClZXzsd{B2e2mwZzDQc4x6NSiJbIWsJHR6eiliYYOgd5Vje3S{YYTlJI1m[XO3cnXkJIFnfGW{IES1JI1qdnW2ZYOgZpkhU2mwYYPlMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiSVO1NEA:KDBwME[wPEDPxE1w NWj5bW5yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5NFI4OTlpPkK5PVAzPzF7PD;hQi=>
HT-29 cells MYTGeY5kfGmxbjDhd5NigQ>? M2Ox[|MhcA>? NFfzWWhKdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGmwIHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDNiaILzJIJ6KEqFLUGgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6wNkDPxE1w NWD4cXlORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOVcyPTVpPkOwNFU4OTV3PD;hQi=>
Sf9 cells NYC0Z3dGTnWwY4Tpc44h[XO|YYm= M3nOZmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMjCoNUB1dyBzNEmwJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEWzJO69VS5? M1mxVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyME[3N|U5Lz5|MEC2O|M2QDxxYU6=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

15121855 24174874 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

22684244 25215174 15121855 19840952 15140398 19880522
体内研究 在局部贫血前,使用Staurosporine(0.1-10 ng)预处理局部贫血沙鼠和大鼠模型,可防止神经元损伤,这种作用存在剂量依赖性,说明CAl锥体细胞死亡涉及PKC。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
细胞实验:[4]
- 合并
  • Cell lines: PC12
  • Concentrations: 溶于DMSO,终浓度为1 μM
  • Incubation Time: ~32 小时
  • Method: 使用Staurosporine处理细胞32小时。细胞在4%多聚甲醛中混合,然后使用DNA结合染料Hoechst 33342染色。在荧光照明下观察细胞,测定凋亡细胞百分数。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 短暂局部贫血的雄性Mongolian沙鼠或雄性 Wistar大鼠
  • Dosages: ~10 ng
  • Administration: 定向处理到海马双边CA1子域
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 466.53
化学式

C28H26N4O3

CAS号 62996-74-1
储存条件 粉状
溶于溶剂
别名 Antibiotic AM-2282, STS

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID