PKC
信号通路图
研究领域
抑制剂选择性比较
PKC产品
| 产品目录 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| S1055 |
Enzastaurin (LY317615)Enzastaurin (LY317615)是一种有效的PKCβ选择性抑制剂，无细胞试验中IC50为6 nM，比作用于PKCα，PKCγ和PKCε选择性高6到20倍。Phase 3。 |
The protein kinase C (PKC)–specific inhibitor enzastaurin
induces apoptosis of lupus B cells and prevents lupus development
in Sle mice. C, Levels of serum IgG anti–double-stranded DNA
(anti-dsDNA) and antihistone/anti-dsDNA autoantibodies from
vehicle-treated control mice and enzastaurin-treated mice, as analyzed
by enzyme-linked immunosorbent assay. Bars in A–C show the mean
SD of 3 independent experiments. D, Representative immunofluorescent
images of IgG deposition (top) and glomeruli (bottom) in kidney
sections from Sle1.Sle3 mice treated with vehicle or enzastaurin. Original
magnification 20 (top); 40 (bottom). PAS periodic acid–Schiff.
|
|
| S2791 |
SotrastaurinSotrastaurin是一种有效的，选择性的泛PKC抑制剂，最有效作用于PKCθ，无细胞试验中Ki为0.22 nM；对PKCζ没有活性。Phase 2。 |
Lysates from H3122 and MGH006 cells treated with 1 µM PMA in the presence or absence of 0.3 µM sotrastaurin (SOT) were fractionated. Immunoblotting was performed with the indicated antibodies.
|
|
| S1421 |
StaurosporineStaurosporine是一种有效的PKC抑制剂，在无细胞试验中作用于PKCα，PKCγ 和PKCη，IC50分别为2 nM，5 nM 和 4 nM，对PKCδ(20 nM)和PKCε(73 nM作用较弱，对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。 |
Caspase-8, 9, 3, 6, PARP, and cleaved PARP were detected in POTEG overexpressed cells and control cells with or without STS treatment.
|
|
| S2911 |
Go 6983Go 6983是一种pan-PKC抑制剂，作用于PKCα， PKCβ， PKCγ和PKCδ，IC50分别为7 nM， 7 nM， 6 nM和10 nM，对PKCζ作用稍弱，抑制PKCμ活性。 |
(a) In vitro kinase reactions were performed by using GSTp531-64 (FP267) as substrate and GST-CK1δ (FP449) as enzyme, or using (b) GST-CK1δ375–428 (FP1183) as substrate and recombinant PKCα as enzyme in combination with one of three PKC-specific inhibitors (Go-6983, enzastaurin, and bisindolylmaleimide I (BIM I), respectively). Data are presented as normalized bar graph.
|
|
| S7208 |
Bisindolylmaleimide I (GF109203X)Bisindolylmaleimide I (GF109203X)是一种有效的PKC抑制剂，作用于PKCα，PKCβI，PKCβII和PKCγ，无细胞试验中IC50分别为20 nM， 17 nM， 16nM和20 nM，作用于PKC比作用于EGFR，PDGFR和胰岛素受体选择性高3000倍以上。 |
Effect of MAPK and PKC inhibitors on Nrf2 expression in CWA-treated macrophages.
|
|
| S1292New |
Chelerythrine ChlorideChelerythrine是一种有效的，选择性的PKC拮抗剂， IC50为0.66 mM。 |
||
| S7207 |
Bisindolylmaleimide IX (Ro 31-8220 Mesylate)Bisindolylmaleimide IX (Ro 31-8220 Mesylate)是一种pan-PKC抑制剂，作用于PKC-α， PKC-βI， PKC-βII， PKC-γ和PKC-ε，IC50分别为5 nM， 24 nM， 14 nM， 27 nM和24 nM，也有效抑制MAPKAP-K1b， MSK1， GSK3β和S6K1。 |
||
| S2554 |
DaphnetinDaphnetin是天然香豆素衍生物，是一种蛋白激酶抑制剂，抑制EGFR， PKA和PKC，IC50分别为7.67 μM， 9.33 μM和25.01 μM，且具有抗炎和抗氧化剂活动性。 |
C57/BL6 mice (5 mice/group) were intraperitoneally injected with daphnetin (DFN, 5 mg/kg) or DMSO, and then challenged with LPS (37.5 mg/kg) or saline. 16 h after LPS challenge, mice were sacrificed, and then lung and serum were collected. c, Representative photomicrographs showed H&E staining of lung tissue.
|
|
| S4066 |
Dequalinium ChlorideDequalinium Chloride是一种PKC抑制剂，IC50为7-18 μM，同时也是一种选择性蜂毒明肽敏感型钾离子通道阻断剂，IC50为1.1 μM. |
||
| S2391 |
QuercetinQuercetin是蔬菜水果和白酒中的天然黄酮类化合物，是一种体外重组Sirt1蛋白的激活剂同时也是PI3K抑制剂，IC50为2.4 – 5.4 μM。 |
After starved in serum-free medium for 24h,A549 cells incubated with the indicated concentrations of Quercetin for 3h,followed by 20-minute stimolation of 100ng/ml EGF. |
|
| S3661 |
2-Methoxy-1,4-naphthoquinone2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells. |
||
| S2327 |
MyricitrinMyricitrin是一种化学化合物，可从Myrica cerifera (Bayberry属, 小乔木，原产于北美)的根皮中分离得到。 |
||
| S7663 |
Ruboxistaurin (LY333531 HCl)LY333531是特异性β-蛋白激酶C的抑制剂。能竞争性地、可逆地抑制PKCβ1和PKCβ2，IC50分别为4.7和5.9 nM。 |
||
| S7119 |
Go6976Go6976是一种强效的PKC抑制剂，其对PKC (老鼠大脑), PKCα, and PKCβ1的IC50分别为7.9nM，2.3 nM, 和 6.2 nM 。此外，也是JAK2 和 Flt3的强抑制剂。 |
Go6976 abolished the anticalcification activity of CST by promoting the ossification of cultured VSMCs. Rat VSMCs werecultured in growth medium or calcification medium in the presence or absence of 10 7mol/L of CST or in calcification medium containing10 7mol/L of CST and pretreated with different doses of Go6976 (0.01, 0.1 and 0.5lmol/L) for 30 min. On day 7 (A, n=4) and 12 (B,n=4), VSMCs were harvested for the determination of ALP activity (A) using an ALP assay kit and calcium content (B) using the OCPCmethod. The VSMCs cultured with calcification medium containing 10 7mol/L of CST were pretreated with different doses of Go6976 (0.01and 0.1lmol/L) for 30 min. On day 6, VSMCs were harvested for the detection of expression of OCN (C, D, n=6). Data from at least threeindependent experiments are presented as the mean SEM, and representative images are shown.*P<.05 and †P<.01. Ctrl, control groupin vitro;b-GP,b-glycerophosphate group;b-GP+CST,b-glycerophosphate plus 10 7mol/L cortistatin group;b-GP+CST+Go6976,b-glycerophosphate plus 10 7mol/L cortistatin plus Go6976 group.
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| S8064 |
Midostaurin (PKC412)Midostaurin (PKC412)是一种多靶点激酶抑制剂，作用于PKCα/β/γ， Syk， Flk-1， Akt， PKA， c-Kit， c-Fgr， c-Src， FLT3， PDFRβ和VEGFR1/2，IC50为80-500 nM。 |
(f and g) Induction of apoptosis in HMC-1.1 and -1.2, respectively. Columns represent the mean of three independent experiments and the bars represent the standard error. ctrl, control; mido, midostaurin.
|
|
| s9101New |
IngenolIngenol, a plant-derived diterpenoid, is a weak PKC activator and possesses important anticancer activity. |
||
| S3819 |
Decursinol angelateDecursinol angelate is a cytotoxic and protein kinase C activating agent from the root of Angelica gigas. |
| 产品目录 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| S1055 |
Enzastaurin (LY317615)Enzastaurin (LY317615)是一种有效的PKCβ选择性抑制剂，无细胞试验中IC50为6 nM，比作用于PKCα，PKCγ和PKCε选择性高6到20倍。Phase 3。 |
The protein kinase C (PKC)–specific inhibitor enzastaurin
induces apoptosis of lupus B cells and prevents lupus development
in Sle mice. C, Levels of serum IgG anti–double-stranded DNA
(anti-dsDNA) and antihistone/anti-dsDNA autoantibodies from
vehicle-treated control mice and enzastaurin-treated mice, as analyzed
by enzyme-linked immunosorbent assay. Bars in A–C show the mean
SD of 3 independent experiments. D, Representative immunofluorescent
images of IgG deposition (top) and glomeruli (bottom) in kidney
sections from Sle1.Sle3 mice treated with vehicle or enzastaurin. Original
magnification 20 (top); 40 (bottom). PAS periodic acid–Schiff.
|
|
| S2791 |
SotrastaurinSotrastaurin是一种有效的，选择性的泛PKC抑制剂，最有效作用于PKCθ，无细胞试验中Ki为0.22 nM；对PKCζ没有活性。Phase 2。 |
Lysates from H3122 and MGH006 cells treated with 1 µM PMA in the presence or absence of 0.3 µM sotrastaurin (SOT) were fractionated. Immunoblotting was performed with the indicated antibodies.
|
|
| S1421 |
StaurosporineStaurosporine是一种有效的PKC抑制剂，在无细胞试验中作用于PKCα，PKCγ 和PKCη，IC50分别为2 nM，5 nM 和 4 nM，对PKCδ(20 nM)和PKCε(73 nM作用较弱，对PKCζ (1086 nM)的活性很低。同时 对其他的激酶PKA, PKG, S6K, CaMKII, 等也显示抑制活性。Phase 3。 |
Caspase-8, 9, 3, 6, PARP, and cleaved PARP were detected in POTEG overexpressed cells and control cells with or without STS treatment.
|
|
| S2911 |
Go 6983Go 6983是一种pan-PKC抑制剂，作用于PKCα， PKCβ， PKCγ和PKCδ，IC50分别为7 nM， 7 nM， 6 nM和10 nM，对PKCζ作用稍弱，抑制PKCμ活性。 |
(a) In vitro kinase reactions were performed by using GSTp531-64 (FP267) as substrate and GST-CK1δ (FP449) as enzyme, or using (b) GST-CK1δ375–428 (FP1183) as substrate and recombinant PKCα as enzyme in combination with one of three PKC-specific inhibitors (Go-6983, enzastaurin, and bisindolylmaleimide I (BIM I), respectively). Data are presented as normalized bar graph.
|
|
| S7208 |
Bisindolylmaleimide I (GF109203X)Bisindolylmaleimide I (GF109203X)是一种有效的PKC抑制剂，作用于PKCα，PKCβI，PKCβII和PKCγ，无细胞试验中IC50分别为20 nM， 17 nM， 16nM和20 nM，作用于PKC比作用于EGFR，PDGFR和胰岛素受体选择性高3000倍以上。 |
Effect of MAPK and PKC inhibitors on Nrf2 expression in CWA-treated macrophages.
|
|
| S1292New |
Chelerythrine ChlorideChelerythrine是一种有效的，选择性的PKC拮抗剂， IC50为0.66 mM。 |
||
| S7207 |
Bisindolylmaleimide IX (Ro 31-8220 Mesylate)Bisindolylmaleimide IX (Ro 31-8220 Mesylate)是一种pan-PKC抑制剂，作用于PKC-α， PKC-βI， PKC-βII， PKC-γ和PKC-ε，IC50分别为5 nM， 24 nM， 14 nM， 27 nM和24 nM，也有效抑制MAPKAP-K1b， MSK1， GSK3β和S6K1。 |
||
| S2554 |
DaphnetinDaphnetin是天然香豆素衍生物，是一种蛋白激酶抑制剂，抑制EGFR， PKA和PKC，IC50分别为7.67 μM， 9.33 μM和25.01 μM，且具有抗炎和抗氧化剂活动性。 |
C57/BL6 mice (5 mice/group) were intraperitoneally injected with daphnetin (DFN, 5 mg/kg) or DMSO, and then challenged with LPS (37.5 mg/kg) or saline. 16 h after LPS challenge, mice were sacrificed, and then lung and serum were collected. c, Representative photomicrographs showed H&E staining of lung tissue.
|
|
| S4066 |
Dequalinium ChlorideDequalinium Chloride是一种PKC抑制剂，IC50为7-18 μM，同时也是一种选择性蜂毒明肽敏感型钾离子通道阻断剂，IC50为1.1 μM. |
||
| S2391 |
QuercetinQuercetin是蔬菜水果和白酒中的天然黄酮类化合物，是一种体外重组Sirt1蛋白的激活剂同时也是PI3K抑制剂，IC50为2.4 – 5.4 μM。 |
After starved in serum-free medium for 24h,A549 cells incubated with the indicated concentrations of Quercetin for 3h,followed by 20-minute stimolation of 100ng/ml EGF. |
|
| S3661 |
2-Methoxy-1,4-naphthoquinone2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells. |
||
| S2327 |
MyricitrinMyricitrin是一种化学化合物，可从Myrica cerifera (Bayberry属, 小乔木，原产于北美)的根皮中分离得到。 |
||
| S7663 |
Ruboxistaurin (LY333531 HCl)LY333531是特异性β-蛋白激酶C的抑制剂。能竞争性地、可逆地抑制PKCβ1和PKCβ2，IC50分别为4.7和5.9 nM。 |
||
| S7119 |
Go6976Go6976是一种强效的PKC抑制剂，其对PKC (老鼠大脑), PKCα, and PKCβ1的IC50分别为7.9nM，2.3 nM, 和 6.2 nM 。此外，也是JAK2 和 Flt3的强抑制剂。 |
Go6976 abolished the anticalcification activity of CST by promoting the ossification of cultured VSMCs. Rat VSMCs werecultured in growth medium or calcification medium in the presence or absence of 10 7mol/L of CST or in calcification medium containing10 7mol/L of CST and pretreated with different doses of Go6976 (0.01, 0.1 and 0.5lmol/L) for 30 min. On day 7 (A, n=4) and 12 (B,n=4), VSMCs were harvested for the determination of ALP activity (A) using an ALP assay kit and calcium content (B) using the OCPCmethod. The VSMCs cultured with calcification medium containing 10 7mol/L of CST were pretreated with different doses of Go6976 (0.01and 0.1lmol/L) for 30 min. On day 6, VSMCs were harvested for the detection of expression of OCN (C, D, n=6). Data from at least threeindependent experiments are presented as the mean SEM, and representative images are shown.*P<.05 and †P<.01. Ctrl, control groupin vitro;b-GP,b-glycerophosphate group;b-GP+CST,b-glycerophosphate plus 10 7mol/L cortistatin group;b-GP+CST+Go6976,b-glycerophosphate plus 10 7mol/L cortistatin plus Go6976 group.
|
|
| S8064 |
Midostaurin (PKC412)Midostaurin (PKC412)是一种多靶点激酶抑制剂，作用于PKCα/β/γ， Syk， Flk-1， Akt， PKA， c-Kit， c-Fgr， c-Src， FLT3， PDFRβ和VEGFR1/2，IC50为80-500 nM。 |
(f and g) Induction of apoptosis in HMC-1.1 and -1.2, respectively. Columns represent the mean of three independent experiments and the bars represent the standard error. ctrl, control; mido, midostaurin.
|
| 产品目录 | 产品描述 | 文献引用 | 实验数据 |
|---|---|---|---|
| s9101New |
IngenolIngenol, a plant-derived diterpenoid, is a weak PKC activator and possesses important anticancer activity. |
||
| S3819 |
Decursinol angelateDecursinol angelate is a cytotoxic and protein kinase C activating agent from the root of Angelica gigas. |
