Go6976

别名: PD406976

Go6976 (PD406976)是一种强效的PKC抑制剂,其对PKC (老鼠大脑), PKCα, and PKCβ1的IC50分别为7.9nM,2.3 nM, 和 6.2 nM 。此外,也是JAK2Flt3的强抑制剂。

Go6976 Chemical Structure

Go6976 Chemical Structure

CAS: 136194-77-9

规格 价格 库存 购买数量
5mg 1615.81 现货
25mg 4053.02 现货
100mg 10565.1 现货
1g 40868.1 现货
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相关信号通路图

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
primary microglial cells Function assay 1.3 uM Suppression of IFN-gamma-induced PKCalpha/beta2 nuclear translocation in Sprague-Dawley rat primary microglial cells at 1.3 uM by Western blotting 17395590
primary microglial cells Function assay 1.3 uM Suppression of IFN-gamma-induced IFN-gamma regulatory factor 1 nuclear translocation in Sprague-Dawley rat primary microglial cells at 1.3 uM by Western blotting 17395590
Vero Cytotoxicity assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured on day 5 post dose by MTS/PMS based microscopic analysis, CC50=6μM. 28689975
primary microglial cells Function assay Inhibition of IFN-gamma-induced IFN-gamma regulatory factor levels in Sprague-Dawley rat primary microglial cells by Western blotting 17395590
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB1643 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB-EBc1 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
U-2 OS qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for U-2 OS cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Rh41 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Saos-2 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells 29435139
SK-N-SH qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-SH cells 29435139
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生物活性

产品描述 Go6976 (PD406976)是一种强效的PKC抑制剂,其对PKC (老鼠大脑), PKCα, and PKCβ1的IC50分别为7.9nM,2.3 nM, 和 6.2 nM 。此外,也是JAK2Flt3的强抑制剂。
靶点
JAK2 [2]
(Cell-free assay)
FLT3 [2]
(Cell-free assay)
PKCα [1]
(Cell-free assay)
PKCβ1 [1]
(Cell-free assay)
PKC [1]
(Rat brain)
2.3 nM 6.2 nM 7.9 nM
体外研究(In Vitro)
体外研究活性 Go6976对Ca(2+)不依赖性PKC亚型δ,ε,和ζ的激酶活性没有作用。[1]除了WT JAK2,Go6976也会抑制突变型血液恶性肿瘤(JAK2 V617F 和 TEL-JAK2),且具有抗突变型FLT3的活性。在AML细胞中,Go6976将FLT3-ITD样品中的存活率降低到对照组的55%,FLT3-WT样品中降低为69%。[2] Go 6976有效抑制苔藓抑素1,肿瘤坏死因子α和白细胞介素6诱导的HIV-1。[3]
激酶实验 PKC 活性试验
简而言之,对于测量大鼠脑中的PKCα,PKCβ1 和 PKC,200 μl试验混合物包含50 mM HEPES (pH7.5),5 mM MgCl2,1 mM EDTA,1.25 mM EGTA,1.32 mM CaC12,1 mM 二硫苏糖醇,1 μg 磷脂酰丝氨酸,0.2 μg 二油精,40 μg 组蛋白Hi,10 μM [γ-32P]ATP (1 μCi/ml),和5-10 单位 (pmol of Pi/min) PKC。加入[γ-32P]ATP开始测定,在30℃下培养5分钟,加入2 ml 8.5% H3PO4停止测定,通过0.45-μm硝化纤维素过滤器过滤,并使用闪烁计数器进行评估。
细胞实验 细胞系 原代 AML 细胞
浓度 1 μM
孵育时间 48小时
方法 细胞以2×105每点悬浮在200 μl RPMI/10% FCS。研究下的抑制剂以近似浓度加入,细胞在37℃,5% CO2下培养48小时。MTS活性通过CellTiter试剂盒根据制造商说明进行测量。结果表示为对照组(没有抑制剂的细胞组)的百分比。
实验图片 检测方法 检测指标 实验图片 PMID
Western blot p-EGFR / EGFR / p-AKT / AKT / p-ERK / ERK / p-S6 / S6 23229345
Immunofluorescence p62 / LAMP2 29794026
体内研究(In Vivo)
体内研究活性 Go6976 (2.5 mg/kg i.p.),作为PKD抑制剂,通过抑制MAPKs活化作用以减少TNF-α产生,而有效防止LPS/D:-GalN诱导的急性肝损伤,并显著提高LPS/D-GalN攻击的小鼠的存活率。[4]
动物实验 Animal Models LPS/D-GalN-攻击的小鼠
Dosages 2.5 mg/kg
Administration i.p.

化学信息&溶解度

分子量 377.42 分子式

C24H18N4O

CAS号 136194-77-9 SDF Download Go6976 SDF
Smiles CN1C2=CC=CC=C2C3=C4C(=C5C6=CC=CC=C6N(C5=C31)CCC#N)CNC4=O
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 44.7 mg/mL ( (118.43 mM) ;DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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